Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64494.t1 | XP_003595357.1 | 96.1 | 255 | 6 | 2 | 1 | 251 | 1 | 255 | 1.90E-137 | 498.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64494.t1 | Q8GYX2 | 38.6 | 267 | 125 | 9 | 1 | 249 | 1 | 246 | 1.2e-34 | 148.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64494.t1 | G7IIQ7 | 96.1 | 255 | 6 | 2 | 1 | 251 | 1 | 255 | 1.4e-137 | 498.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene64494 | 0.8024 | 5.71E-49 | -1.69E-46 |
| MS.gene057423 | MS.gene64494 | 0.810215 | 1.29E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene64494 | 0.807688 | 4.47E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64494.t1 | MTR_2g042970 | 96.078 | 255 | 6 | 2 | 1 | 251 | 1 | 255 | 0.0 | 502 |
| MS.gene64494.t1 | MTR_4g124930 | 55.344 | 262 | 94 | 12 | 1 | 251 | 1 | 250 | 2.04e-78 | 237 |
| MS.gene64494.t1 | MTR_4g124930 | 54.286 | 245 | 90 | 12 | 1 | 238 | 1 | 230 | 2.08e-68 | 211 |
| MS.gene64494.t1 | MTR_5g013770 | 52.222 | 90 | 30 | 4 | 172 | 250 | 76 | 163 | 2.61e-15 | 71.6 |
| MS.gene64494.t1 | MTR_3g108290 | 48.101 | 79 | 40 | 1 | 171 | 248 | 342 | 420 | 5.05e-15 | 74.3 |
| MS.gene64494.t1 | MTR_8g008840 | 57.407 | 54 | 22 | 1 | 174 | 226 | 29 | 82 | 5.09e-13 | 64.3 |
| MS.gene64494.t1 | MTR_3g073960 | 59.574 | 47 | 18 | 1 | 182 | 227 | 89 | 135 | 6.07e-13 | 65.1 |
| MS.gene64494.t1 | MTR_1g103500 | 40.566 | 106 | 50 | 4 | 118 | 222 | 192 | 285 | 9.03e-13 | 67.0 |
| MS.gene64494.t1 | MTR_4g099050 | 61.818 | 55 | 17 | 2 | 183 | 236 | 73 | 124 | 5.63e-12 | 62.4 |
| MS.gene64494.t1 | MTR_1g010140 | 35.821 | 134 | 82 | 4 | 118 | 250 | 213 | 343 | 2.45e-11 | 63.2 |
| MS.gene64494.t1 | MTR_8g022950 | 59.091 | 44 | 17 | 1 | 183 | 225 | 39 | 82 | 6.87e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64494.t1 | AT5G20700 | 38.806 | 268 | 125 | 10 | 1 | 250 | 1 | 247 | 1.92e-43 | 147 |
| MS.gene64494.t1 | AT1G74940 | 36.047 | 258 | 121 | 10 | 1 | 251 | 2 | 222 | 4.77e-34 | 122 |
| MS.gene64494.t1 | AT1G19200 | 33.597 | 253 | 133 | 12 | 6 | 251 | 4 | 228 | 4.19e-21 | 89.0 |
| MS.gene64494.t1 | AT1G19200 | 34.361 | 227 | 115 | 11 | 31 | 251 | 17 | 215 | 5.03e-20 | 85.9 |
| MS.gene64494.t1 | AT1G22160 | 64.706 | 51 | 17 | 1 | 182 | 231 | 77 | 127 | 7.57e-16 | 72.8 |
| MS.gene64494.t1 | AT1G53885 | 53.571 | 56 | 25 | 1 | 171 | 225 | 30 | 85 | 1.45e-15 | 71.6 |
| MS.gene64494.t1 | AT1G53903 | 53.571 | 56 | 25 | 1 | 171 | 225 | 30 | 85 | 1.45e-15 | 71.6 |
| MS.gene64494.t1 | AT1G78020 | 59.184 | 49 | 19 | 1 | 183 | 230 | 89 | 137 | 3.86e-13 | 65.9 |
| MS.gene64494.t1 | AT5G65040 | 60.870 | 46 | 17 | 1 | 182 | 226 | 56 | 101 | 2.84e-12 | 62.4 |
| MS.gene64494.t1 | AT3G22550 | 28.794 | 257 | 121 | 11 | 8 | 225 | 32 | 265 | 3.10e-12 | 65.1 |
| MS.gene64494.t1 | AT2G44670 | 64.444 | 45 | 15 | 1 | 183 | 226 | 16 | 60 | 3.54e-12 | 61.2 |
| MS.gene64494.t1 | AT5G47060 | 46.835 | 79 | 31 | 3 | 183 | 250 | 97 | 175 | 7.64e-12 | 62.8 |
| MS.gene64494.t1 | AT5G47060 | 46.835 | 79 | 31 | 3 | 183 | 250 | 97 | 175 | 7.64e-12 | 62.8 |
| MS.gene64494.t1 | AT4G17670 | 62.500 | 48 | 17 | 1 | 183 | 229 | 76 | 123 | 1.39e-11 | 61.6 |
Find 33 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAATTCAAACGGGCTTCT+AGG | 0.297546 | 2.4:-47295164 | None:intergenic |
| GTCACATAAGTGTAATCTTC+TGG | 0.307242 | 2.4:-47295416 | None:intergenic |
| CAAAATCATCAACAACAAAA+TGG | 0.342676 | 2.4:+47295362 | MS.gene64494:CDS |
| TTTGCAAATGAGATTCTTGT+TGG | 0.361648 | 2.4:+47295389 | MS.gene64494:CDS |
| GAATTTGGTGGTGTTGGTCT+TGG | 0.362778 | 2.4:+47295224 | MS.gene64494:CDS |
| TAATCCCAGTTGAAAACATT+TGG | 0.378194 | 2.4:-47296058 | None:intergenic |
| AACATTTGGTCCTTTGTGTA+TGG | 0.411067 | 2.4:-47296044 | None:intergenic |
| TCTCCTGATCGATTCGACTT+AGG | 0.418223 | 2.4:-47295320 | None:intergenic |
| ATCTTTGAGCTCAATTCAAA+CGG | 0.420646 | 2.4:-47295173 | None:intergenic |
| TTAGGTGTACAAACATGTTT+CGG | 0.426714 | 2.4:-47295302 | None:intergenic |
| AAAATTATCATCATCATGTT+GGG | 0.449714 | 2.4:+47295053 | None:intergenic |
| AGCTATGAATTTGGTGGTGT+TGG | 0.457246 | 2.4:+47295218 | MS.gene64494:CDS |
| GAGCTAAAGTTTGTGTTGGT+GGG | 0.459975 | 2.4:-47295562 | None:intergenic |
| GTACCCGACAAAATCGTTAT+CGG | 0.467459 | 2.4:-47295270 | None:intergenic |
| TATGATGGCGGTGAAGAAAG+TGG | 0.470889 | 2.4:+47295479 | MS.gene64494:CDS |
| CGAGCTAAAGTTTGTGTTGG+TGG | 0.474487 | 2.4:-47295563 | None:intergenic |
| AAATCCAAGGTTGGATATGA+TGG | 0.475932 | 2.4:-47295593 | None:intergenic |
| TGGTGTTGGTCTTGGAATTG+TGG | 0.476764 | 2.4:+47295232 | MS.gene64494:CDS |
| ACACCTAAGTCGAATCGATC+AGG | 0.488722 | 2.4:+47295317 | MS.gene64494:CDS |
| TCTTTGAGCTCAATTCAAAC+GGG | 0.490170 | 2.4:-47295172 | None:intergenic |
| AGAGAAGTGTAGATCATCAA+TGG | 0.509195 | 2.4:+47296007 | MS.gene64494:CDS |
| TCAAAGATTCAATCACCAAA+AGG | 0.513577 | 2.4:+47295188 | MS.gene64494:CDS |
| GCAAAGACATATTCATGTAC+AGG | 0.528815 | 2.4:+47295654 | MS.gene64494:CDS |
| ATCTAATAAACCTATCACTA+AGG | 0.533202 | 2.4:+47295451 | MS.gene64494:CDS |
| TCCGATTGTGTCGAAAAGCC+CGG | 0.539258 | 2.4:-47295138 | None:intergenic |
| AGCTAAAGTTTGTGTTGGTG+GGG | 0.539629 | 2.4:-47295561 | None:intergenic |
| GCTCGAGCTAAAGTTTGTGT+TGG | 0.541739 | 2.4:-47295566 | None:intergenic |
| TCGTTCAAAGCAAATAATGA+TGG | 0.550315 | 2.4:+47295974 | MS.gene64494:CDS |
| AGCAAATAATGATGGATGAG+AGG | 0.563262 | 2.4:+47295982 | MS.gene64494:CDS |
| GAATTGAGAAAATCCAAGGT+TGG | 0.589378 | 2.4:-47295602 | None:intergenic |
| ACATGAATTGAGAAAATCCA+AGG | 0.596600 | 2.4:-47295606 | None:intergenic |
| TGAACCATCATATCCAACCT+TGG | 0.631022 | 2.4:+47295589 | MS.gene64494:CDS |
| ATCAAGTTCTCCATACACAA+AGG | 0.691311 | 2.4:+47296034 | MS.gene64494:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATTTTAATTTTAGATT+TGG | - | chr2.4:47295686-47295705 | None:intergenic | 10.0% |
| !!! | ACAATTTTAATTTTAGATTT+GGG | - | chr2.4:47295685-47295704 | None:intergenic | 10.0% |
| !! | TATTTAGATATATGACTAAT+TGG | + | chr2.4:47295899-47295918 | MS.gene64494:intron | 15.0% |
| !!! | CTAATTGGTAATTTTAATTT+TGG | + | chr2.4:47295914-47295933 | MS.gene64494:intron | 15.0% |
| ! | ATCTAATAAACCTATCACTA+AGG | + | chr2.4:47295451-47295470 | MS.gene64494:CDS | 25.0% |
| ! | CAAAATCATCAACAACAAAA+TGG | + | chr2.4:47295362-47295381 | MS.gene64494:CDS | 25.0% |
| ! | GGATTAAAAAGCTATGAATT+TGG | + | chr2.4:47295209-47295228 | MS.gene64494:CDS | 25.0% |
| ! | TCATAAAAAACCTTAGTGAT+AGG | - | chr2.4:47295464-47295483 | None:intergenic | 25.0% |
| ! | TTAAAAAGCTATGAATTTGG+TGG | + | chr2.4:47295212-47295231 | MS.gene64494:CDS | 25.0% |
| !! | TCATAGCTTTTTAATCCTTT+TGG | - | chr2.4:47295206-47295225 | None:intergenic | 25.0% |
| !!! | ATCACTAAGGTTTTTTATGA+TGG | + | chr2.4:47295464-47295483 | MS.gene64494:CDS | 25.0% |
| !!! | ATTTTAATTTTGGAGTGTAG+AGG | + | chr2.4:47295924-47295943 | MS.gene64494:intron | 25.0% |
| !!! | CTAGTAGTTCTGAAAATTTT+CGG | - | chr2.4:47295100-47295119 | None:intergenic | 25.0% |
| !!! | TGTTGTTGATGATTTTGAAT+TGG | - | chr2.4:47295359-47295378 | None:intergenic | 25.0% |
| !!! | TTTTAATTTTGGAGTGTAGA+GGG | + | chr2.4:47295925-47295944 | MS.gene64494:intron | 25.0% |
| !!! | TTTTCAGAACTACTAGTTTT+CGG | + | chr2.4:47295104-47295123 | MS.gene64494:CDS | 25.0% |
| ACATGAATTGAGAAAATCCA+AGG | - | chr2.4:47295609-47295628 | None:intergenic | 30.0% | |
| ATCTTTGAGCTCAATTCAAA+CGG | - | chr2.4:47295176-47295195 | None:intergenic | 30.0% | |
| TAATCCCAGTTGAAAACATT+TGG | - | chr2.4:47296061-47296080 | None:intergenic | 30.0% | |
| TCAAAGATTCAATCACCAAA+AGG | + | chr2.4:47295188-47295207 | MS.gene64494:CDS | 30.0% | |
| TCGTTCAAAGCAAATAATGA+TGG | + | chr2.4:47295974-47295993 | MS.gene64494:CDS | 30.0% | |
| TTAGGTGTACAAACATGTTT+CGG | - | chr2.4:47295305-47295324 | None:intergenic | 30.0% | |
| TTGTGCAAAAAGAATCTTCA+TGG | + | chr2.4:47295632-47295651 | MS.gene64494:CDS | 30.0% | |
| TTTGCAAATGAGATTCTTGT+TGG | + | chr2.4:47295389-47295408 | MS.gene64494:CDS | 30.0% | |
| ! | AAATCCGATAACGATTTTGT+CGG | + | chr2.4:47295266-47295285 | MS.gene64494:CDS | 30.0% |
| ! | AATGTTTTCAACTGGGATTA+TGG | + | chr2.4:47296061-47296080 | MS.gene64494:CDS | 30.0% |
| ! | TTTTGAATTGGAATCGTGAT+CGG | - | chr2.4:47295347-47295366 | None:intergenic | 30.0% |
| !!! | ACTAAGGTTTTTTATGATGG+CGG | + | chr2.4:47295467-47295486 | MS.gene64494:CDS | 30.0% |
| !!! | TTTAATTTTGGAGTGTAGAG+GGG | + | chr2.4:47295926-47295945 | MS.gene64494:intron | 30.0% |
| AAATCCAAGGTTGGATATGA+TGG | - | chr2.4:47295596-47295615 | None:intergenic | 35.0% | |
| AACATTTGGTCCTTTGTGTA+TGG | - | chr2.4:47296047-47296066 | None:intergenic | 35.0% | |
| ACCTCAAAAAACAACAACGT+TGG | + | chr2.4:47295524-47295543 | MS.gene64494:CDS | 35.0% | |
| ATCAAGTTCTCCATACACAA+AGG | + | chr2.4:47296034-47296053 | MS.gene64494:CDS | 35.0% | |
| GAATTGAGAAAATCCAAGGT+TGG | - | chr2.4:47295605-47295624 | None:intergenic | 35.0% | |
| GCAAAGACATATTCATGTAC+AGG | + | chr2.4:47295654-47295673 | MS.gene64494:CDS | 35.0% | |
| GTCACATAAGTGTAATCTTC+TGG | - | chr2.4:47295419-47295438 | None:intergenic | 35.0% | |
| TCTTTGAGCTCAATTCAAAC+GGG | - | chr2.4:47295175-47295194 | None:intergenic | 35.0% | |
| ! | AAAATTTTCGGATCAAGTGC+TGG | - | chr2.4:47295088-47295107 | None:intergenic | 35.0% |
| ! | AAATTTTCGGATCAAGTGCT+GGG | - | chr2.4:47295087-47295106 | None:intergenic | 35.0% |
| ! | AAGGACCAAATGTTTTCAAC+TGG | + | chr2.4:47296053-47296072 | MS.gene64494:CDS | 35.0% |
| ! | AATCCGATAACGATTTTGTC+GGG | + | chr2.4:47295267-47295286 | MS.gene64494:CDS | 35.0% |
| ! | AGAGAAGTGTAGATCATCAA+TGG | + | chr2.4:47296007-47296026 | MS.gene64494:CDS | 35.0% |
| ! | AGGACCAAATGTTTTCAACT+GGG | + | chr2.4:47296054-47296073 | MS.gene64494:CDS | 35.0% |
| !! | AGCAAATAATGATGGATGAG+AGG | + | chr2.4:47295982-47296001 | MS.gene64494:CDS | 35.0% |
| !! | TTCGTCGCTAAATTTTAGAG+AGG | - | chr2.4:47295721-47295740 | None:intergenic | 35.0% |
| !!! | ACCAACGTTGTTGTTTTTTG+AGG | - | chr2.4:47295528-47295547 | None:intergenic | 35.0% |
| GTACCCGACAAAATCGTTAT+CGG | - | chr2.4:47295273-47295292 | None:intergenic | 40.0% | |
| TGAACCATCATATCCAACCT+TGG | + | chr2.4:47295589-47295608 | MS.gene64494:CDS | 40.0% | |
| ! | AGCTAAAGTTTGTGTTGGTG+GGG | - | chr2.4:47295564-47295583 | None:intergenic | 40.0% |
| ! | GAGCTAAAGTTTGTGTTGGT+GGG | - | chr2.4:47295565-47295584 | None:intergenic | 40.0% |
| !! | AGCTATGAATTTGGTGGTGT+TGG | + | chr2.4:47295218-47295237 | MS.gene64494:CDS | 40.0% |
| ACACCTAAGTCGAATCGATC+AGG | + | chr2.4:47295317-47295336 | MS.gene64494:CDS | 45.0% | |
| CGAGCTAAAGTTTGTGTTGG+TGG | - | chr2.4:47295566-47295585 | None:intergenic | 45.0% | |
| GCTCGAGCTAAAGTTTGTGT+TGG | - | chr2.4:47295569-47295588 | None:intergenic | 45.0% | |
| ! | CTCAATTCAAACGGGCTTCT+AGG | - | chr2.4:47295167-47295186 | None:intergenic | 45.0% |
| ! | TAACGATTTTGTCGGGTACG+AGG | + | chr2.4:47295274-47295293 | MS.gene64494:CDS | 45.0% |
| !! | GAATTTGGTGGTGTTGGTCT+TGG | + | chr2.4:47295224-47295243 | MS.gene64494:CDS | 45.0% |
| !! | GTTTTCGGCGAAAGTACAAC+CGG | + | chr2.4:47295119-47295138 | MS.gene64494:CDS | 45.0% |
| !! | TATGATGGCGGTGAAGAAAG+TGG | + | chr2.4:47295479-47295498 | MS.gene64494:CDS | 45.0% |
| !! | TCTCCTGATCGATTCGACTT+AGG | - | chr2.4:47295323-47295342 | None:intergenic | 45.0% |
| !! | TGGTGTTGGTCTTGGAATTG+TGG | + | chr2.4:47295232-47295251 | MS.gene64494:CDS | 45.0% |
| !! | TTTTCGGCGAAAGTACAACC+GGG | + | chr2.4:47295120-47295139 | MS.gene64494:CDS | 45.0% |
| TCCGATTGTGTCGAAAAGCC+CGG | - | chr2.4:47295141-47295160 | None:intergenic | 50.0% | |
| ! | ACCGGGCTTTTCGACACAAT+CGG | + | chr2.4:47295137-47295156 | MS.gene64494:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 47295068 | 47296091 | 47295068 | ID=MS.gene64494 |
| chr2.4 | mRNA | 47295068 | 47296091 | 47295068 | ID=MS.gene64494.t1;Parent=MS.gene64494 |
| chr2.4 | exon | 47295068 | 47295675 | 47295068 | ID=MS.gene64494.t1.exon1;Parent=MS.gene64494.t1 |
| chr2.4 | CDS | 47295068 | 47295675 | 47295068 | ID=cds.MS.gene64494.t1;Parent=MS.gene64494.t1 |
| chr2.4 | exon | 47295944 | 47296091 | 47295944 | ID=MS.gene64494.t1.exon2;Parent=MS.gene64494.t1 |
| chr2.4 | CDS | 47295944 | 47296091 | 47295944 | ID=cds.MS.gene64494.t1;Parent=MS.gene64494.t1 |
| Gene Sequence |
| Protein sequence |
