Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05758.t1 | KEH34366.1 | 64.3 | 140 | 37 | 3 | 1 | 138 | 91 | 219 | 4.10E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05758.t1 | A0A072UY95 | 64.3 | 140 | 37 | 3 | 1 | 138 | 91 | 219 | 2.9e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05758 | MS.gene66907 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene05758 | MS.gene91022 | 0.828253 | 9.96E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05758.t1 | MTR_3g465350 | 73.571 | 140 | 24 | 3 | 1 | 138 | 91 | 219 | 9.01e-64 | 194 |
| MS.gene05758.t1 | MTR_3g465380 | 45.263 | 95 | 51 | 1 | 5 | 99 | 110 | 203 | 3.59e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTAGCACAGCTGATTAA+TGG | 0.293544 | 3.1:+49157838 | MS.gene05758:CDS |
| CTTCCCTCAATTTCTTCACT+TGG | 0.325891 | 3.1:-49157792 | None:intergenic |
| GCATAGGCGGTGTCCACATT+TGG | 0.336759 | 3.1:-49158066 | None:intergenic |
| CATAGAAATAGATCGTCAAT+AGG | 0.337816 | 3.1:-49158151 | None:intergenic |
| CCAACATCAAAGAGTACTTT+TGG | 0.343632 | 3.1:-49157547 | None:intergenic |
| TTGTTCTTACCTTGATCATA+TGG | 0.344596 | 3.1:-49157919 | None:intergenic |
| CAACAGCATGGAATTGTTCA+TGG | 0.372107 | 3.1:+49158111 | MS.gene05758:CDS |
| AACTAGCACAGCTGATTAAT+GGG | 0.393662 | 3.1:+49157839 | MS.gene05758:CDS |
| GTCCACATTTGGCTTTGTAA+TGG | 0.416169 | 3.1:-49158055 | None:intergenic |
| ATAATTCGAGTCTAGATCAT+TGG | 0.449966 | 3.1:-49157887 | None:intergenic |
| ATAGAAATAGATCGTCAATA+GGG | 0.456204 | 3.1:-49158150 | None:intergenic |
| CATGAACAATTCCATGCTGT+TGG | 0.471592 | 3.1:-49158110 | None:intergenic |
| GTTTCAAATCCATATGATCA+AGG | 0.476271 | 3.1:+49157910 | MS.gene05758:CDS |
| ATGAACAATTCCATGCTGTT+GGG | 0.480845 | 3.1:-49158109 | None:intergenic |
| CCAAAAGTACTCTTTGATGT+TGG | 0.482742 | 3.1:+49157547 | MS.gene05758:CDS |
| GCATGGAATTGTTCATGGAT+TGG | 0.489116 | 3.1:+49158116 | MS.gene05758:CDS |
| AAATCCAAGTGAAGAAATTG+AGG | 0.503725 | 3.1:+49157788 | MS.gene05758:CDS |
| AATGTGGACACCGCCTATGC+GGG | 0.515675 | 3.1:+49158069 | MS.gene05758:CDS |
| CTCAGCTTGTTGATTGACAT+AGG | 0.522116 | 3.1:-49157815 | None:intergenic |
| GTTGGAGCGTGAGAAATTGA+TGG | 0.529938 | 3.1:+49157565 | MS.gene05758:CDS |
| GGAATTGTTCATGGATTGGA+TGG | 0.532597 | 3.1:+49158120 | MS.gene05758:CDS |
| AAAGAAGTACTTCACAAAGA+AGG | 0.541781 | 3.1:+49157608 | MS.gene05758:CDS |
| GTTTCGTGCTCATCCCGCAT+AGG | 0.549638 | 3.1:-49158082 | None:intergenic |
| TGAGAAATTGATGGATAAAG+TGG | 0.554684 | 3.1:+49157574 | MS.gene05758:CDS |
| AATCCAAGTGAAGAAATTGA+GGG | 0.585556 | 3.1:+49157789 | MS.gene05758:CDS |
| GAGTCTAGATCATTGGACAT+TGG | 0.604288 | 3.1:-49157880 | None:intergenic |
| GACCATTACAAAGCCAAATG+TGG | 0.607277 | 3.1:+49158053 | MS.gene05758:CDS |
| CGAAACAGAACCCAACAGCA+TGG | 0.608229 | 3.1:+49158099 | MS.gene05758:CDS |
| TTAATTATCAGTTGAAACTG+TGG | 0.609589 | 3.1:+49157759 | MS.gene05758:intron |
| TCGTGCTCATCCCGCATAGG+CGG | 0.614893 | 3.1:-49158079 | None:intergenic |
| CTATGTCAATCAACAAGCTG+AGG | 0.632240 | 3.1:+49157816 | MS.gene05758:CDS |
| AAATGTGGACACCGCCTATG+CGG | 0.637544 | 3.1:+49158068 | MS.gene05758:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACATTTTTGTTTTTGAAA+AGG | - | chr3.1:49157989-49158008 | None:intergenic | 15.0% |
| !! | AGTTTCAACTGATAATTAAT+GGG | - | chr3.1:49157758-49157777 | None:intergenic | 20.0% |
| ! | ATAGAAATAGATCGTCAATA+GGG | - | chr3.1:49158153-49158172 | None:intergenic | 25.0% |
| ! | CAGTTTCAACTGATAATTAA+TGG | - | chr3.1:49157759-49157778 | None:intergenic | 25.0% |
| ! | TTAATTATCAGTTGAAACTG+TGG | + | chr3.1:49157759-49157778 | MS.gene05758:intron | 25.0% |
| !! | CATAGCTCAATATATTGATT+AGG | + | chr3.1:49157692-49157711 | MS.gene05758:intron | 25.0% |
| AAATCCAAGTGAAGAAATTG+AGG | + | chr3.1:49157788-49157807 | MS.gene05758:CDS | 30.0% | |
| AATCCAAGTGAAGAAATTGA+GGG | + | chr3.1:49157789-49157808 | MS.gene05758:CDS | 30.0% | |
| ATAATTCGAGTCTAGATCAT+TGG | - | chr3.1:49157890-49157909 | None:intergenic | 30.0% | |
| CATAGAAATAGATCGTCAAT+AGG | - | chr3.1:49158154-49158173 | None:intergenic | 30.0% | |
| GTTTCAAATCCATATGATCA+AGG | + | chr3.1:49157910-49157929 | MS.gene05758:CDS | 30.0% | |
| TTGTTCTTACCTTGATCATA+TGG | - | chr3.1:49157922-49157941 | None:intergenic | 30.0% | |
| ! | TGAGAAATTGATGGATAAAG+TGG | + | chr3.1:49157574-49157593 | MS.gene05758:CDS | 30.0% |
| !! | AAAGAAGTACTTCACAAAGA+AGG | + | chr3.1:49157608-49157627 | MS.gene05758:CDS | 30.0% |
| AACTAGCACAGCTGATTAAT+GGG | + | chr3.1:49157839-49157858 | MS.gene05758:CDS | 35.0% | |
| ATGAACAATTCCATGCTGTT+GGG | - | chr3.1:49158112-49158131 | None:intergenic | 35.0% | |
| !! | CCAAAAGTACTCTTTGATGT+TGG | + | chr3.1:49157547-49157566 | MS.gene05758:CDS | 35.0% |
| !!! | CCAACATCAAAGAGTACTTT+TGG | - | chr3.1:49157550-49157569 | None:intergenic | 35.0% |
| CAACAGCATGGAATTGTTCA+TGG | + | chr3.1:49158111-49158130 | MS.gene05758:CDS | 40.0% | |
| CATGAACAATTCCATGCTGT+TGG | - | chr3.1:49158113-49158132 | None:intergenic | 40.0% | |
| CTATGTCAATCAACAAGCTG+AGG | + | chr3.1:49157816-49157835 | MS.gene05758:CDS | 40.0% | |
| CTTCCCTCAATTTCTTCACT+TGG | - | chr3.1:49157795-49157814 | None:intergenic | 40.0% | |
| GAACTAGCACAGCTGATTAA+TGG | + | chr3.1:49157838-49157857 | MS.gene05758:CDS | 40.0% | |
| GACCATTACAAAGCCAAATG+TGG | + | chr3.1:49158053-49158072 | MS.gene05758:CDS | 40.0% | |
| GAGTCTAGATCATTGGACAT+TGG | - | chr3.1:49157883-49157902 | None:intergenic | 40.0% | |
| GCATGGAATTGTTCATGGAT+TGG | + | chr3.1:49158116-49158135 | MS.gene05758:CDS | 40.0% | |
| !! | CTCAGCTTGTTGATTGACAT+AGG | - | chr3.1:49157818-49157837 | None:intergenic | 40.0% |
| !! | GGAATTGTTCATGGATTGGA+TGG | + | chr3.1:49158120-49158139 | MS.gene05758:CDS | 40.0% |
| !! | GTCCACATTTGGCTTTGTAA+TGG | - | chr3.1:49158058-49158077 | None:intergenic | 40.0% |
| GTTGGAGCGTGAGAAATTGA+TGG | + | chr3.1:49157565-49157584 | MS.gene05758:CDS | 45.0% | |
| !!! | ATTTATTTTTTAAATTACAT+AGG | + | chr3.1:49158031-49158050 | MS.gene05758:intron | 5.0% |
| AAATGTGGACACCGCCTATG+CGG | + | chr3.1:49158068-49158087 | MS.gene05758:CDS | 50.0% | |
| CGAAACAGAACCCAACAGCA+TGG | + | chr3.1:49158099-49158118 | MS.gene05758:CDS | 50.0% | |
| AATGTGGACACCGCCTATGC+GGG | + | chr3.1:49158069-49158088 | MS.gene05758:CDS | 55.0% | |
| GCATAGGCGGTGTCCACATT+TGG | - | chr3.1:49158069-49158088 | None:intergenic | 55.0% | |
| GTTTCGTGCTCATCCCGCAT+AGG | - | chr3.1:49158085-49158104 | None:intergenic | 55.0% | |
| TCGTGCTCATCCCGCATAGG+CGG | - | chr3.1:49158082-49158101 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 49157497 | 49158174 | 49157497 | ID=MS.gene05758 |
| chr3.1 | mRNA | 49157497 | 49158174 | 49157497 | ID=MS.gene05758.t1;Parent=MS.gene05758 |
| chr3.1 | exon | 49157497 | 49157629 | 49157497 | ID=MS.gene05758.t1.exon1;Parent=MS.gene05758.t1 |
| chr3.1 | CDS | 49157497 | 49157629 | 49157497 | ID=cds.MS.gene05758.t1;Parent=MS.gene05758.t1 |
| chr3.1 | exon | 49157770 | 49157931 | 49157770 | ID=MS.gene05758.t1.exon2;Parent=MS.gene05758.t1 |
| chr3.1 | CDS | 49157770 | 49157931 | 49157770 | ID=cds.MS.gene05758.t1;Parent=MS.gene05758.t1 |
| chr3.1 | exon | 49158053 | 49158174 | 49158053 | ID=MS.gene05758.t1.exon3;Parent=MS.gene05758.t1 |
| chr3.1 | CDS | 49158053 | 49158174 | 49158053 | ID=cds.MS.gene05758.t1;Parent=MS.gene05758.t1 |
| Gene Sequence |
| Protein sequence |
