Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057597.t1 | XP_004515231.1 | 94.6 | 74 | 4 | 0 | 1 | 74 | 970 | 1043 | 2.90E-33 | 150.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057597.t1 | G7JP36 | 97.3 | 74 | 2 | 0 | 1 | 74 | 963 | 1036 | 2.1e-33 | 150.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057597 | MS.gene057598 | 0.811989 | 5.31E-51 | -1.69E-46 |
| MS.gene057597 | MS.gene96242 | 0.801481 | 8.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGTTTAACTATTACATA+AGG | - | 33144:9763-9782 | MS.gene057597:CDS | 15.0% |
| !!! | AAAATTCAAGTTGATAGCTA+TGG | + | 33144:9746-9765 | MS.gene057597:intergenic | 25.0% |
| ACACAACGTCTAATAAATTG+CGG | - | 33144:9910-9929 | MS.gene057597:CDS | 30.0% | |
| ! | TTCAGTCCAGAACTTTTAAA+AGG | - | 33144:9864-9883 | MS.gene057597:CDS | 30.0% |
| !! | ACTACACCTTTTAAAAGTTC+TGG | + | 33144:9873-9892 | MS.gene057597:intergenic | 30.0% |
| !! | AGAACTTTTAAAAGGTGTAG+TGG | - | 33144:9872-9891 | MS.gene057597:CDS | 30.0% |
| !! | TTTAAAAGTTCTGGACTGAA+GGG | + | 33144:9864-9883 | MS.gene057597:intergenic | 30.0% |
| !!! | TTTTAAAAGTTCTGGACTGA+AGG | + | 33144:9865-9884 | MS.gene057597:intergenic | 30.0% |
| AATCAGGTCTGATGAAAGTT+TGG | - | 33144:9808-9827 | MS.gene057597:CDS | 35.0% | |
| ATCAGGTCTGATGAAAGTTT+GGG | - | 33144:9809-9828 | MS.gene057597:CDS | 35.0% | |
| ATTACATAAGGATGCTTCAG+AGG | - | 33144:9775-9794 | MS.gene057597:CDS | 35.0% | |
| GCAATTTATTAGACGTTGTG+TGG | + | 33144:9911-9930 | MS.gene057597:intergenic | 35.0% | |
| ! | TTAAAAGGTGTAGTGGAAGT+TGG | - | 33144:9879-9898 | MS.gene057597:CDS | 35.0% |
| CAACGTCTAATAAATTGCGG+CGG | - | 33144:9913-9932 | MS.gene057597:CDS | 40.0% | |
| TATTAGACGTTGTGTGGAAG+TGG | + | 33144:9905-9924 | MS.gene057597:intergenic | 40.0% | |
| AGTTTGGGAATCCGTTTCTC+AGG | - | 33144:9824-9843 | MS.gene057597:CDS | 45.0% | |
| GTTTGGGAATCCGTTTCTCA+GGG | - | 33144:9825-9844 | MS.gene057597:CDS | 45.0% | |
| CTGGACTGAAGGGATATAGC+TGG | + | 33144:9854-9873 | MS.gene057597:intergenic | 50.0% | |
| GACTGAAGGGATATAGCTGG+AGG | + | 33144:9851-9870 | MS.gene057597:intergenic | 50.0% | |
| !! | CAGAGGCATCAGAAGCAATC+AGG | - | 33144:9792-9811 | MS.gene057597:CDS | 50.0% |
| TAGCTGGAGGCCCTGAGAAA+CGG | + | 33144:9838-9857 | MS.gene057597:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33144 | gene | 9733 | 9958 | 9733 | ID=MS.gene057597 |
| 33144 | mRNA | 9733 | 9958 | 9733 | ID=MS.gene057597.t1;Parent=MS.gene057597 |
| 33144 | exon | 9733 | 9958 | 9733 | ID=MS.gene057597.t1.exon1;Parent=MS.gene057597.t1 |
| 33144 | CDS | 9733 | 9958 | 9733 | ID=cds.MS.gene057597.t1;Parent=MS.gene057597.t1 |
| Gene Sequence |
| Protein sequence |
