AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057848

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057848.t1 MTR_8g096990 70.096 418 117 5 1 413 17 431 0.0 579
MS.gene057848.t1 MTR_5g080960 68.810 420 121 5 1 413 17 433 0.0 570
MS.gene057848.t1 MTR_5g080890 68.333 420 123 5 1 413 17 433 0.0 565
MS.gene057848.t1 MTR_5g044770 68.345 417 125 5 1 413 17 430 0.0 563
MS.gene057848.t1 MTR_8g038250 68.632 424 97 7 1 413 17 415 0.0 559
MS.gene057848.t1 MTR_8g038280 66.114 422 131 5 1 413 17 435 0.0 543
MS.gene057848.t1 MTR_8g038310 60.766 418 141 5 1 415 18 415 0.0 511
MS.gene057848.t1 MTR_6g090290 61.137 422 151 6 2 413 18 436 1.26e-168 480
MS.gene057848.t1 MTR_4g109760 61.440 389 117 6 31 413 37 398 7.59e-150 431
MS.gene057848.t1 MTR_3g028120 52.518 417 184 7 9 413 24 438 9.17e-145 419
MS.gene057848.t1 MTR_2g067830 56.098 410 164 12 16 413 1 406 1.34e-137 400
MS.gene057848.t1 MTR_5g038360 50.824 425 192 9 2 413 16 436 2.07e-135 395
MS.gene057848.t1 MTR_6g088760 54.082 392 164 8 1 379 17 405 7.40e-134 394
MS.gene057848.t1 MTR_2g105300 52.826 407 154 13 16 410 1 381 7.78e-124 363
MS.gene057848.t1 MTR_0024s0040 48.675 415 193 9 14 413 1 410 9.71e-121 357
MS.gene057848.t1 MTR_5g044570 45.540 426 187 10 2 413 17 411 8.08e-119 352
MS.gene057848.t1 MTR_0036s0110 40.515 427 229 11 5 413 23 442 3.14e-100 305
MS.gene057848.t1 MTR_5g008070 42.105 418 217 11 5 412 23 425 8.33e-100 304
MS.gene057848.t1 MTR_0036s0070 41.162 413 221 12 10 413 27 426 1.87e-99 303
MS.gene057848.t1 MTR_0036s0050 42.308 416 221 11 10 413 27 435 4.17e-99 302
MS.gene057848.t1 MTR_2g059460 42.005 419 194 12 7 413 25 406 7.52e-96 293
MS.gene057848.t1 MTR_2g059490 42.823 418 195 12 7 412 24 409 2.64e-94 290
MS.gene057848.t1 MTR_5g008150 40.385 416 210 10 6 413 27 412 1.77e-87 272
MS.gene057848.t1 MTR_5g008110 40.263 380 210 8 41 413 46 415 6.12e-86 268
MS.gene057848.t1 MTR_1g060750 37.594 399 216 12 24 413 2 376 2.70e-75 239
MS.gene057848.t1 MTR_3g097150 35.782 422 237 13 11 413 42 448 3.16e-72 233
MS.gene057848.t1 MTR_4g045857 42.254 284 129 7 7 285 24 277 1.06e-58 194
MS.gene057848.t1 MTR_5g008090 39.865 296 167 8 127 413 21 314 1.21e-57 191
MS.gene057848.t1 MTR_1g115210 31.555 431 262 13 1 411 69 486 5.10e-55 189
MS.gene057848.t1 MTR_7g117430 35.196 358 206 13 68 411 139 484 7.00e-54 186
MS.gene057848.t1 MTR_1g039270 30.536 429 252 15 12 411 58 469 2.71e-52 182
MS.gene057848.t1 MTR_6g088765 43.515 239 101 6 145 380 3 210 9.88e-49 166
MS.gene057848.t1 MTR_1g111350 32.487 394 238 14 30 411 90 467 1.02e-48 172
MS.gene057848.t1 MTR_4g008110 74.336 113 8 1 144 256 46 137 4.06e-46 155
MS.gene057848.t1 MTR_6g085010 32.951 349 214 11 70 411 156 491 1.27e-43 159
MS.gene057848.t1 MTR_3g046300 32.468 385 234 12 52 413 79 460 2.28e-40 149
MS.gene057848.t1 MTR_2g028190 33.515 367 220 13 61 411 93 451 9.06e-40 148
MS.gene057848.t1 MTR_4g007740 29.825 399 247 16 33 412 94 478 4.08e-39 146
MS.gene057848.t1 MTR_5g091130 29.524 420 254 12 31 412 139 554 2.04e-38 145
MS.gene057848.t1 MTR_3g053710 33.425 362 211 14 69 411 116 466 6.81e-38 143
MS.gene057848.t1 MTR_8g103880 29.793 386 241 14 40 411 86 455 6.25e-37 140
MS.gene057848.t1 MTR_7g080230 27.918 437 261 20 14 412 11 431 1.13e-31 125
MS.gene057848.t1 MTR_7g105850 30.110 455 232 24 24 413 34 467 5.49e-30 121
MS.gene057848.t1 MTR_2g055840 27.968 379 234 15 62 411 5 373 5.39e-28 114
MS.gene057848.t1 MTR_3g068000 28.238 386 220 15 68 411 71 441 2.10e-27 114
MS.gene057848.t1 MTR_3g053980 29.752 363 233 13 61 411 133 485 3.46e-26 110
MS.gene057848.t1 MTR_3g065160 26.329 395 232 16 58 413 54 428 1.62e-25 107
MS.gene057848.t1 MTR_2g070430 27.273 440 263 17 12 408 25 450 4.31e-25 107
MS.gene057848.t1 MTR_6g084710 26.963 382 243 13 60 414 181 553 1.99e-24 105
MS.gene057848.t1 MTR_0945s0010 41.600 125 69 4 290 412 1 123 3.50e-23 95.5
MS.gene057848.t1 MTR_2g103340 44.444 117 21 1 4 120 33 105 4.66e-23 96.3
MS.gene057848.t1 MTR_7g080230 28.895 353 201 18 94 412 25 361 1.50e-22 99.0
MS.gene057848.t1 MTR_2g084550 29.474 380 215 18 69 412 76 438 2.88e-22 99.0
MS.gene057848.t1 MTR_1g029810 27.177 379 219 22 69 413 56 411 3.25e-22 98.2
MS.gene057848.t1 MTR_1g029810 27.177 379 219 22 69 413 56 411 4.04e-22 98.2
MS.gene057848.t1 MTR_4g056420 28.877 374 224 19 69 412 76 437 8.00e-22 97.8
MS.gene057848.t1 MTR_1g037630 28.873 426 259 20 9 412 26 429 1.15e-21 96.7
MS.gene057848.t1 MTR_7g062360 25.570 395 241 14 56 411 45 425 1.29e-21 96.7
MS.gene057848.t1 MTR_5g077850 25.199 377 229 14 71 413 82 439 1.49e-21 96.7
MS.gene057848.t1 MTR_5g016260 25.768 423 243 20 21 415 56 435 1.80e-21 97.1
MS.gene057848.t1 MTR_5g016260 25.768 423 243 20 21 415 56 435 2.66e-21 96.7
MS.gene057848.t1 MTR_1g078240 27.851 377 226 19 69 412 68 431 2.73e-21 95.9
MS.gene057848.t1 MTR_7g021510 25.858 379 231 18 69 412 71 434 2.89e-21 95.9
MS.gene057848.t1 MTR_1g078240 27.394 376 228 19 69 412 68 430 8.05e-21 94.7
MS.gene057848.t1 MTR_3g096930 24.936 389 233 18 62 413 70 436 1.64e-20 93.6
MS.gene057848.t1 MTR_4g023650 25.063 395 240 17 55 413 47 421 2.77e-20 92.8
MS.gene057848.t1 MTR_5g061690 27.954 347 205 18 77 399 108 433 3.22e-20 93.2
MS.gene057848.t1 MTR_4g095270 25.538 372 231 17 68 415 78 427 4.15e-20 92.8
MS.gene057848.t1 MTR_4g095270 25.538 372 231 17 68 415 78 427 4.68e-20 92.8
MS.gene057848.t1 MTR_3g014290 29.775 356 217 16 71 412 81 417 5.85e-20 91.7
MS.gene057848.t1 MTR_6g084710 27.365 296 191 10 60 345 181 462 2.37e-19 90.5
MS.gene057848.t1 MTR_4g023630 25.250 400 233 20 55 413 47 421 1.46e-18 87.4
MS.gene057848.t1 MTR_1g078240 28.387 310 184 16 69 353 68 364 2.82e-18 86.3
MS.gene057848.t1 MTR_7g102750 26.146 371 238 15 69 412 80 441 3.48e-18 86.7
MS.gene057848.t1 MTR_8g039540 27.594 424 268 19 9 412 30 434 1.24e-17 84.7
MS.gene057848.t1 MTR_1g061940 26.933 375 232 18 69 412 82 445 3.67e-17 83.6
MS.gene057848.t1 MTR_5g077920 22.955 379 237 12 71 413 69 428 7.81e-17 82.4
MS.gene057848.t1 MTR_7g102750 27.362 307 192 13 69 353 80 377 1.21e-15 79.0
MS.gene057848.t1 MTR_5g061690 28.028 289 177 15 124 399 24 294 2.57e-15 77.4
MS.gene057848.t1 MTR_8g075090 26.039 361 223 19 76 411 118 459 1.52e-14 75.5
MS.gene057848.t1 MTR_5g022880 25.905 359 228 16 76 411 118 461 6.28e-14 73.9
MS.gene057848.t1 MTR_3g100500 27.841 352 209 16 69 398 96 424 5.17e-13 70.9
MS.gene057848.t1 MTR_8g075090 25.857 321 203 16 76 375 118 424 2.59e-12 68.6
MS.gene057848.t1 MTR_2g037710 26.066 211 131 9 209 411 12 205 3.71e-12 65.9
MS.gene057848.t1 MTR_3g087780 27.129 317 196 15 113 411 9 308 1.69e-11 65.9
MS.gene057848.t1 MTR_3g087780 27.129 317 196 15 113 411 45 344 3.28e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057848.t1 AT1G64830 46.472 411 209 8 10 413 24 430 8.49e-124 365
MS.gene057848.t1 AT5G33340 47.445 411 204 8 11 413 30 436 1.24e-119 355
MS.gene057848.t1 AT2G35615 44.313 422 211 8 12 412 26 444 5.91e-109 328
MS.gene057848.t1 AT1G31450 43.091 427 218 10 5 412 22 442 4.01e-99 303
MS.gene057848.t1 AT2G28010 40.144 416 191 15 10 415 28 395 9.96e-83 259
MS.gene057848.t1 AT2G28040 39.716 423 200 17 1 415 19 394 6.12e-79 249
MS.gene057848.t1 AT2G28040 40.338 414 192 17 10 415 19 385 1.73e-78 248
MS.gene057848.t1 AT2G28030 41.972 355 172 13 71 415 61 391 1.01e-73 236
MS.gene057848.t1 AT2G28220 37.081 418 207 13 10 415 28 401 5.31e-72 231
MS.gene057848.t1 AT2G28225 39.554 359 179 14 71 415 67 401 4.60e-65 214
MS.gene057848.t1 AT2G03200 35.374 441 233 16 6 413 39 460 5.22e-65 215
MS.gene057848.t1 AT1G01300 36.490 359 202 13 68 412 139 485 3.42e-56 192
MS.gene057848.t1 AT3G20015 31.066 441 250 16 4 411 51 470 6.98e-54 186
MS.gene057848.t1 AT3G61820 35.989 364 201 14 68 411 132 483 1.34e-50 177
MS.gene057848.t1 AT3G18490 32.361 377 231 13 46 411 137 500 2.74e-50 177
MS.gene057848.t1 AT1G25510 33.708 356 207 12 68 411 145 483 8.63e-48 170
MS.gene057848.t1 AT2G23945 27.578 446 259 16 10 413 27 450 8.83e-45 161
MS.gene057848.t1 AT4G30030 33.067 375 218 15 54 411 61 419 7.70e-43 155
MS.gene057848.t1 AT5G10760 31.604 424 254 16 7 411 58 464 9.27e-43 156
MS.gene057848.t1 AT5G10770 33.800 429 240 19 13 412 61 474 4.14e-42 154
MS.gene057848.t1 AT3G25700 30.353 425 252 18 23 414 39 452 1.50e-39 147
MS.gene057848.t1 AT1G79720 35.870 368 189 18 71 411 135 482 8.57e-39 145
MS.gene057848.t1 AT4G30040 29.371 429 251 19 12 406 17 427 1.70e-38 144
MS.gene057848.t1 AT2G42980 30.890 382 236 13 56 411 145 524 1.06e-37 143
MS.gene057848.t1 AT3G59080 30.971 381 237 14 56 412 155 533 5.71e-37 141
MS.gene057848.t1 AT3G12700 30.663 362 229 12 69 412 104 461 2.80e-33 130
MS.gene057848.t1 AT5G36260 30.263 380 208 20 69 412 76 434 5.85e-32 127
MS.gene057848.t1 AT3G59080 28.723 376 216 12 56 412 155 497 1.71e-28 117
MS.gene057848.t1 AT1G09750 28.684 380 226 14 60 411 86 448 1.98e-27 113
MS.gene057848.t1 AT2G39710 27.968 379 218 16 68 406 62 425 6.26e-27 112
MS.gene057848.t1 AT3G54400 27.937 383 237 15 51 412 61 425 2.67e-26 110
MS.gene057848.t1 AT1G66180 25.330 379 225 16 71 413 72 428 2.50e-25 107
MS.gene057848.t1 AT5G02190 26.542 373 221 16 80 411 82 442 3.75e-25 107
MS.gene057848.t1 AT1G65240 29.412 374 216 19 69 412 72 427 1.82e-24 105
MS.gene057848.t1 AT3G02740 26.738 374 228 17 69 412 83 440 2.01e-24 105
MS.gene057848.t1 AT3G02740 27.049 366 221 17 77 412 16 365 4.63e-24 103
MS.gene057848.t1 AT5G45120 27.670 412 211 19 71 411 83 478 5.82e-24 103
MS.gene057848.t1 AT5G43100 26.108 406 239 16 36 412 45 418 9.73e-24 103
MS.gene057848.t1 AT4G33490 24.810 395 248 15 52 413 35 413 1.75e-23 102
MS.gene057848.t1 AT5G43100 26.108 406 239 16 36 412 45 418 2.26e-23 102
MS.gene057848.t1 AT5G37540 25.668 374 230 15 71 411 80 438 1.56e-21 96.7
MS.gene057848.t1 AT4G12920 26.963 382 229 17 50 411 36 387 3.84e-21 94.7
MS.gene057848.t1 AT5G22850 28.533 375 225 18 69 412 79 441 2.30e-20 93.6
MS.gene057848.t1 AT2G17760 27.184 412 246 22 32 411 60 449 2.82e-20 93.2
MS.gene057848.t1 AT3G50050 24.523 367 238 12 68 413 90 438 3.29e-20 93.6
MS.gene057848.t1 AT5G07030 28.810 420 255 19 4 399 43 442 4.66e-20 92.4
MS.gene057848.t1 AT1G05840 26.385 379 227 16 69 412 78 439 6.78e-20 92.0
MS.gene057848.t1 AT4G33490 27.241 290 180 11 52 324 32 307 2.32e-19 89.7
MS.gene057848.t1 AT1G44130 27.068 399 239 18 49 413 21 401 2.72e-19 89.7
MS.gene057848.t1 AT1G49050 23.099 355 240 12 88 414 49 398 1.14e-18 87.8
MS.gene057848.t1 AT1G49050 23.099 355 240 12 88 414 222 571 1.21e-18 88.6
MS.gene057848.t1 AT3G52500 28.465 404 206 23 69 412 88 468 5.26e-18 86.3
MS.gene057848.t1 AT2G36670 24.661 369 245 13 69 412 98 458 1.25e-17 85.1
MS.gene057848.t1 AT2G36670 24.931 361 238 13 77 412 111 463 2.76e-17 84.3
MS.gene057848.t1 AT2G36670 24.931 361 238 13 77 412 40 392 4.28e-17 83.2
MS.gene057848.t1 AT2G36670 24.931 361 238 13 77 412 40 392 4.28e-17 83.2
MS.gene057848.t1 AT1G08210 26.064 376 230 16 69 412 82 441 4.71e-17 83.6
MS.gene057848.t1 AT1G08210 26.596 376 228 17 69 412 130 489 5.03e-17 83.6
MS.gene057848.t1 AT1G08210 26.064 376 230 16 69 412 116 475 5.19e-17 83.6
MS.gene057848.t1 AT1G77480 26.075 372 233 19 69 413 65 421 1.96e-16 81.3
MS.gene057848.t1 AT1G77480 26.075 372 233 19 69 413 65 421 2.74e-16 80.9
MS.gene057848.t1 AT3G42550 24.054 370 216 13 61 412 68 390 6.90e-16 79.7
MS.gene057848.t1 AT5G24820 23.604 394 261 13 49 415 23 403 9.23e-16 79.0
MS.gene057848.t1 AT1G08210 27.653 311 182 14 69 352 82 376 1.23e-14 76.3
MS.gene057848.t1 AT5G10080 24.619 459 283 24 1 413 14 455 3.53e-13 71.6
MS.gene057848.t1 AT3G25700 28.497 193 123 7 23 205 39 226 4.24e-13 70.5

Find 83 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGGCATCTTTGGGAATT+TGG 0.131735 4.4:+84380269 MS.gene057848:CDS
GGAGAAGCTGCTGTAGTTTC+TGG 0.204176 4.4:+84379847 MS.gene057848:CDS
CAACTCAATGAAGGCATCTT+TGG 0.206666 4.4:+84380261 MS.gene057848:CDS
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.4:+84379787 MS.gene057848:CDS
CCTAAAACTGTGATAGGTTT+TGG 0.228752 4.4:+84379664 MS.gene057848:CDS
AGCTGGGATCACAGTGGATT+TGG 0.229529 4.4:-84379305 None:intergenic
TCAAAATGTGCAGTGATTAT+AGG 0.251652 4.4:-84380156 None:intergenic
ACTCCGCCATTTAAGTTATA+TGG 0.253439 4.4:+84379361 MS.gene057848:CDS
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.4:+84379786 MS.gene057848:CDS
ACAATACCATATAACTTAAA+TGG 0.265952 4.4:-84379367 None:intergenic
TCATTTCCTAAAACTGTGAT+AGG 0.301005 4.4:+84379658 MS.gene057848:CDS
TGCAAATATACTATTAAGTA+TGG 0.330083 4.4:+84379562 MS.gene057848:CDS
AATCAATTGGGTTCATCAAT+TGG 0.341653 4.4:+84379763 MS.gene057848:CDS
GGTAACATCATGGTTGATTC+AGG 0.351027 4.4:+84379988 MS.gene057848:CDS
AGTGTCACTTATAAATCAAT+TGG 0.353398 4.4:+84379750 MS.gene057848:CDS
AACAAAGTCTACACTCAATA+TGG 0.354389 4.4:-84380366 None:intergenic
AGAATCAATTGTGGTAAAAT+CGG 0.360522 4.4:+84380053 MS.gene057848:CDS
GATGTAAAGTTGCCTTCTAT+TGG 0.362687 4.4:+84380186 MS.gene057848:CDS
AACTCAATGAAGGCATCTTT+GGG 0.371119 4.4:+84380262 MS.gene057848:CDS
AAAATTATTGACGCGGTTGA+TGG 0.384493 4.4:-84379257 None:intergenic
GCTGGGATCACAGTGGATTT+GGG 0.388278 4.4:-84379304 None:intergenic
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.4:+84379724 MS.gene057848:CDS
ACCTTTGTTCCAGTTGCCGA+TGG 0.391252 4.4:+84380210 MS.gene057848:CDS
AGCTCTGGTGTGGTTGGCCT+TGG 0.402590 4.4:+84379718 MS.gene057848:CDS
CAAACAACCCCATCGGCAAC+TGG 0.402765 4.4:-84380219 None:intergenic
ATCACAAGGAGATCTTAGTT+TGG 0.415779 4.4:+84379597 MS.gene057848:CDS
GATTTCAAAGGATTAAAGAT+AGG 0.418086 4.4:-84379457 None:intergenic
TCCACTGTGATCCCAGCTAA+TGG 0.423768 4.4:+84379310 MS.gene057848:CDS
GGAACAAAGGTGCCAATAGA+AGG 0.423964 4.4:-84380198 None:intergenic
CCTTTGTTCCAGTTGCCGAT+GGG 0.435431 4.4:+84380211 MS.gene057848:CDS
GATTAAAGATAGGAGTCGTT+TGG 0.435507 4.4:-84379447 None:intergenic
TTATATGGTATTGTTGATAC+AGG 0.443331 4.4:+84379376 MS.gene057848:CDS
CCCATCGGCAACTGGAACAA+AGG 0.450218 4.4:-84380211 None:intergenic
ATACAGGTAGTGACATGGTT+TGG 0.456250 4.4:+84379392 MS.gene057848:CDS
ACATTGACATTTGTACCATC+TGG 0.457439 4.4:+84380015 MS.gene057848:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.4:+84379625 MS.gene057848:CDS
TTTGGCTCAACAGAATTTGT+TGG 0.471907 4.4:+84380287 MS.gene057848:CDS
ATGCCTTCATTGAGTTGAGA+TGG 0.476032 4.4:-84380255 None:intergenic
ATAAGAAACTCACCATTAGC+TGG 0.477834 4.4:-84379322 None:intergenic
TGGCGGAGTACCGACTGAAT+AGG 0.486137 4.4:-84379347 None:intergenic
GTGTCACTTATAAATCAATT+GGG 0.489201 4.4:+84379751 MS.gene057848:CDS
TCGACGAGCAAACTCAGTTT+CGG 0.501668 4.4:+84379826 MS.gene057848:CDS
AGTCTACACTCAATATGGTT+TGG 0.504081 4.4:-84380361 None:intergenic
GGCAATAGCTCTGGTGTGGT+TGG 0.508970 4.4:+84379712 MS.gene057848:CDS
GATGGAACGACGCATGGCAT+TGG 0.509894 4.4:-84379239 None:intergenic
TGGATTTGGGTGTGTTAGTG+AGG 0.524238 4.4:-84379291 None:intergenic
TCGTTTGAAGGCAATAGCTC+TGG 0.527506 4.4:+84379703 MS.gene057848:CDS
TATACTATTAAGTATGGTGA+TGG 0.534596 4.4:+84379568 MS.gene057848:CDS
CTTTCTAATGCTCTAAACAA+TGG 0.535397 4.4:+84379136 MS.gene057848:CDS
GCTGCTGTAGTTTCTGGTCA+TGG 0.536172 4.4:+84379853 MS.gene057848:CDS
ATCCCATCTCAACTCAATGA+AGG 0.536264 4.4:+84380252 MS.gene057848:CDS
AAGCGCGCAAAGGATCCAAC+TGG 0.537688 4.4:+84380084 MS.gene057848:CDS
TGCCTTCATTGAGTTGAGAT+GGG 0.542809 4.4:-84380254 None:intergenic
ACATTGAAAGCATTTAGTGT+TGG 0.544651 4.4:+84379928 MS.gene057848:CDS
CTTTGTTCCAGTTGCCGATG+GGG 0.547837 4.4:+84380212 MS.gene057848:CDS
TTGTAACTTGAGGATTTCAA+AGG 0.553263 4.4:-84379469 None:intergenic
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.4:+84379788 MS.gene057848:CDS
TATGGTTTGGTACAATCAGT+AGG 0.559170 4.4:-84380348 None:intergenic
GCTCAACAGAATTTGTTGGT+TGG 0.561992 4.4:+84380291 MS.gene057848:CDS
GCGGTTGATGGAACGACGCA+TGG 0.566473 4.4:-84379245 None:intergenic
TCATAATTTAGAATCAATTG+TGG 0.576270 4.4:+84380044 MS.gene057848:CDS
GGTTAAACTGAAGCGCGCAA+AGG 0.584688 4.4:+84380074 MS.gene057848:CDS
TAAGAAACTCACCATTAGCT+GGG 0.587813 4.4:-84379321 None:intergenic
AATTGATTTATAAGTGACAC+TGG 0.588132 4.4:-84379748 None:intergenic
CTCGTCGAGTTAGACGACTC+TGG 0.588889 4.4:-84379811 None:intergenic
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.4:+84379766 MS.gene057848:CDS
GGATTTGGGTGTGTTAGTGA+GGG 0.600669 4.4:-84379290 None:intergenic
ACGTTAAACATACCACCAGT+TGG 0.605174 4.4:-84380099 None:intergenic
GTGACACTGGTCCATTTCCA+AGG 0.606926 4.4:-84379735 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.4:-84379638 None:intergenic
TTTCTTATGACCTATTCAGT+CGG 0.625475 4.4:+84379337 MS.gene057848:CDS
AGCTAAACAAACAACCCCAT+CGG 0.626429 4.4:-84380226 None:intergenic
TGTTGATACAGGTAGTGACA+TGG 0.630223 4.4:+84379387 MS.gene057848:CDS
TGGTTGTAACAATGTTCACA+AGG 0.636110 4.4:-84379427 None:intergenic
AAATTATGATAAATACCAGA+TGG 0.639824 4.4:-84380030 None:intergenic
CGCGCAAAGGATCCAACTGG+TGG 0.648060 4.4:+84380087 MS.gene057848:CDS
TGAAGGCAATAGCTCTGGTG+TGG 0.649662 4.4:+84379708 MS.gene057848:CDS
ATACCATATAACTTAAATGG+CGG 0.658785 4.4:-84379364 None:intergenic
TAGTGTTGATGGTAACATCA+TGG 0.667211 4.4:+84379978 MS.gene057848:CDS
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.4:-84379641 None:intergenic
GGTGATGGATCAATATCACA+AGG 0.695175 4.4:+84379583 MS.gene057848:CDS
GCATGGCATTGGTAACACGT+TGG 0.699855 4.4:-84379228 None:intergenic
ACCATTAGCTGGGATCACAG+TGG 0.732956 4.4:-84379311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATGATAAATACCAGA+TGG - chr4.4:84380033-84380052 None:intergenic 20.0%
!! ACAATACCATATAACTTAAA+TGG - chr4.4:84379370-84379389 None:intergenic 20.0%
!! GTTGTATAGTAAAAAATTTG+TGG - chr4.4:84379910-84379929 None:intergenic 20.0%
!! TGCAAATATACTATTAAGTA+TGG + chr4.4:84379562-84379581 MS.gene057848:CDS 20.0%
!!! AATATTGTTTTTGTAACTTG+AGG - chr4.4:84379482-84379501 None:intergenic 20.0%
!!! AGTTTTATCTTTACTACAAA+AGG - chr4.4:84379542-84379561 None:intergenic 20.0%
!!! TCATAATTTAGAATCAATTG+TGG + chr4.4:84380044-84380063 MS.gene057848:CDS 20.0%
!!! TTACATTTTTTAGACAAACA+AGG - chr4.4:84379505-84379524 None:intergenic 20.0%
! AGAATCAATTGTGGTAAAAT+CGG + chr4.4:84380053-84380072 MS.gene057848:CDS 25.0%
! ATACCATATAACTTAAATGG+CGG - chr4.4:84379367-84379386 None:intergenic 25.0%
! CTTTGTAAAAATTATTGACG+CGG - chr4.4:84379267-84379286 None:intergenic 25.0%
! GATTTCAAAGGATTAAAGAT+AGG - chr4.4:84379460-84379479 None:intergenic 25.0%
! TATACTATTAAGTATGGTGA+TGG + chr4.4:84379568-84379587 MS.gene057848:CDS 25.0%
! TTATATGGTATTGTTGATAC+AGG + chr4.4:84379376-84379395 MS.gene057848:CDS 25.0%
!! AATTGATTTATAAGTGACAC+TGG - chr4.4:84379751-84379770 None:intergenic 25.0%
!! AGAAGAGTAGAATTTTCAAA+TGG + chr4.4:84379955-84379974 MS.gene057848:CDS 25.0%
!!! AGTGTCACTTATAAATCAAT+TGG + chr4.4:84379750-84379769 MS.gene057848:CDS 25.0%
!!! GTGTCACTTATAAATCAATT+GGG + chr4.4:84379751-84379770 MS.gene057848:CDS 25.0%
AAATGAAATAGAAGAACCAG+AGG - chr4.4:84379644-84379663 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG + chr4.4:84379763-84379782 MS.gene057848:CDS 30.0%
GAAAGTTCTCTTATTGTTTG+GGG + chr4.4:84379788-84379807 MS.gene057848:CDS 30.0%
GGAAAGTTCTCTTATTGTTT+GGG + chr4.4:84379787-84379806 MS.gene057848:CDS 30.0%
TCAAAATGTGCAGTGATTAT+AGG - chr4.4:84380159-84380178 None:intergenic 30.0%
TCATTTCCTAAAACTGTGAT+AGG + chr4.4:84379658-84379677 MS.gene057848:CDS 30.0%
TGGAAAGTTCTCTTATTGTT+TGG + chr4.4:84379786-84379805 MS.gene057848:CDS 30.0%
TTGTAACTTGAGGATTTCAA+AGG - chr4.4:84379472-84379491 None:intergenic 30.0%
TTTCTTATGACCTATTCAGT+CGG + chr4.4:84379337-84379356 MS.gene057848:CDS 30.0%
! ATAATCACTGCACATTTTGA+AGG + chr4.4:84380159-84380178 MS.gene057848:CDS 30.0%
! CTTTCTAATGCTCTAAACAA+TGG + chr4.4:84379136-84379155 MS.gene057848:CDS 30.0%
!! AAAAATACAGTGTCGTTTGA+AGG + chr4.4:84379691-84379710 MS.gene057848:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG + chr4.4:84379928-84379947 MS.gene057848:CDS 30.0%
!! TTTTCAAATGGTAGTGTTGA+TGG + chr4.4:84379967-84379986 MS.gene057848:CDS 30.0%
!!! TGTGGATCTTTTTTCACTAA+AGG - chr4.4:84379892-84379911 None:intergenic 30.0%
AAAATTATTGACGCGGTTGA+TGG - chr4.4:84379260-84379279 None:intergenic 35.0%
ACATTGACATTTGTACCATC+TGG + chr4.4:84380015-84380034 MS.gene057848:CDS 35.0%
ATAAGAAACTCACCATTAGC+TGG - chr4.4:84379325-84379344 None:intergenic 35.0%
ATCACAAGGAGATCTTAGTT+TGG + chr4.4:84379597-84379616 MS.gene057848:CDS 35.0%
GATGTAAAGTTGCCTTCTAT+TGG + chr4.4:84380186-84380205 MS.gene057848:CDS 35.0%
GATTAAAGATAGGAGTCGTT+TGG - chr4.4:84379450-84379469 None:intergenic 35.0%
TAAGAAACTCACCATTAGCT+GGG - chr4.4:84379324-84379343 None:intergenic 35.0%
TGGTTGTAACAATGTTCACA+AGG - chr4.4:84379430-84379449 None:intergenic 35.0%
! ACTCCGCCATTTAAGTTATA+TGG + chr4.4:84379361-84379380 MS.gene057848:CDS 35.0%
! CCAAAACCTATCACAGTTTT+AGG - chr4.4:84379667-84379686 None:intergenic 35.0%
! CCTAAAACTGTGATAGGTTT+TGG + chr4.4:84379664-84379683 MS.gene057848:CDS 35.0%
! TTTGGCTCAACAGAATTTGT+TGG + chr4.4:84380287-84380306 MS.gene057848:CDS 35.0%
!! AACTCAATGAAGGCATCTTT+GGG + chr4.4:84380262-84380281 MS.gene057848:CDS 35.0%
!! TAGTGTTGATGGTAACATCA+TGG + chr4.4:84379978-84379997 MS.gene057848:CDS 35.0%
!! TATGGTTTGGTACAATCAGT+AGG - chr4.4:84380351-84380370 None:intergenic 35.0%
!!! CGTTGGTATTTGTTTTGTGT+AGG - chr4.4:84379214-84379233 None:intergenic 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG - chr4.4:84379210-84379229 None:intergenic 35.0%
ACGTTAAACATACCACCAGT+TGG - chr4.4:84380102-84380121 None:intergenic 40.0%
AGCTAAACAAACAACCCCAT+CGG - chr4.4:84380229-84380248 None:intergenic 40.0%
ATACAGGTAGTGACATGGTT+TGG + chr4.4:84379392-84379411 MS.gene057848:CDS 40.0%
ATCCCATCTCAACTCAATGA+AGG + chr4.4:84380252-84380271 MS.gene057848:CDS 40.0%
CAATTGGGTTCATCAATTGG+TGG + chr4.4:84379766-84379785 MS.gene057848:CDS 40.0%
CTTACATTAGATTCCACCTC+TGG + chr4.4:84379625-84379644 MS.gene057848:CDS 40.0%
TGAAATAGAAGAACCAGAGG+TGG - chr4.4:84379641-84379660 None:intergenic 40.0%
TGTTGATACAGGTAGTGACA+TGG + chr4.4:84379387-84379406 MS.gene057848:CDS 40.0%
! GGTAACATCATGGTTGATTC+AGG + chr4.4:84379988-84380007 MS.gene057848:CDS 40.0%
!! ATGCCTTCATTGAGTTGAGA+TGG - chr4.4:84380258-84380277 None:intergenic 40.0%
!! CAACTCAATGAAGGCATCTT+TGG + chr4.4:84380261-84380280 MS.gene057848:CDS 40.0%
!! GCTCAACAGAATTTGTTGGT+TGG + chr4.4:84380291-84380310 MS.gene057848:CDS 40.0%
!! GGTGATGGATCAATATCACA+AGG + chr4.4:84379583-84379602 MS.gene057848:CDS 40.0%
!! TGAAGGCATCTTTGGGAATT+TGG + chr4.4:84380269-84380288 MS.gene057848:CDS 40.0%
!! TGCCTTCATTGAGTTGAGAT+GGG - chr4.4:84380257-84380276 None:intergenic 40.0%
!!! TTTTGTGTAGGTTGGTAGAG+TGG - chr4.4:84379202-84379221 None:intergenic 40.0%
GGATTTGGGTGTGTTAGTGA+GGG - chr4.4:84379293-84379312 None:intergenic 45.0%
TCGACGAGCAAACTCAGTTT+CGG + chr4.4:84379826-84379845 MS.gene057848:CDS 45.0%
TCGTTTGAAGGCAATAGCTC+TGG + chr4.4:84379703-84379722 MS.gene057848:CDS 45.0%
TGGATTTGGGTGTGTTAGTG+AGG - chr4.4:84379294-84379313 None:intergenic 45.0%
!! GGAACAAAGGTGCCAATAGA+AGG - chr4.4:84380201-84380220 None:intergenic 45.0%
!! GTGGTGATTTTGAAGAGTCG+CGG - chr4.4:84379183-84379202 None:intergenic 45.0%
ACCATTAGCTGGGATCACAG+TGG - chr4.4:84379314-84379333 None:intergenic 50.0%
ACCTTTGTTCCAGTTGCCGA+TGG + chr4.4:84380210-84380229 MS.gene057848:CDS 50.0%
CCTTTGTTCCAGTTGCCGAT+GGG + chr4.4:84380211-84380230 MS.gene057848:CDS 50.0%
CTTTGTTCCAGTTGCCGATG+GGG + chr4.4:84380212-84380231 MS.gene057848:CDS 50.0%
GGAGAAGCTGCTGTAGTTTC+TGG + chr4.4:84379847-84379866 MS.gene057848:CDS 50.0%
GGTTAAACTGAAGCGCGCAA+AGG + chr4.4:84380074-84380093 MS.gene057848:CDS 50.0%
GTGACACTGGTCCATTTCCA+AGG - chr4.4:84379738-84379757 None:intergenic 50.0%
TCCACTGTGATCCCAGCTAA+TGG + chr4.4:84379310-84379329 MS.gene057848:CDS 50.0%
! AGCTGGGATCACAGTGGATT+TGG - chr4.4:84379308-84379327 None:intergenic 50.0%
! GCTGGGATCACAGTGGATTT+GGG - chr4.4:84379307-84379326 None:intergenic 50.0%
!! GCATGGCATTGGTAACACGT+TGG - chr4.4:84379231-84379250 None:intergenic 50.0%
!! GCTGCTGTAGTTTCTGGTCA+TGG + chr4.4:84379853-84379872 MS.gene057848:CDS 50.0%
!! GTGATTTTGAAGAGTCGCGG+TGG - chr4.4:84379180-84379199 None:intergenic 50.0%
!! TGAAGGCAATAGCTCTGGTG+TGG + chr4.4:84379708-84379727 MS.gene057848:CDS 50.0%
AAGCGCGCAAAGGATCCAAC+TGG + chr4.4:84380084-84380103 MS.gene057848:CDS 55.0%
CAAACAACCCCATCGGCAAC+TGG - chr4.4:84380222-84380241 None:intergenic 55.0%
CCCATCGGCAACTGGAACAA+AGG - chr4.4:84380214-84380233 None:intergenic 55.0%
CTCGTCGAGTTAGACGACTC+TGG - chr4.4:84379814-84379833 None:intergenic 55.0%
GATGGAACGACGCATGGCAT+TGG - chr4.4:84379242-84379261 None:intergenic 55.0%
!! GGCAATAGCTCTGGTGTGGT+TGG + chr4.4:84379712-84379731 MS.gene057848:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG + chr4.4:84379724-84379743 MS.gene057848:CDS 55.0%
!! TGGCGGAGTACCGACTGAAT+AGG - chr4.4:84379350-84379369 None:intergenic 55.0%
CGCGCAAAGGATCCAACTGG+TGG + chr4.4:84380087-84380106 MS.gene057848:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG + chr4.4:84379718-84379737 MS.gene057848:CDS 60.0%
!! GCGGTTGATGGAACGACGCA+TGG - chr4.4:84379248-84379267 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 84379127 84380374 84379127 ID=MS.gene057848
chr4.4 mRNA 84379127 84380374 84379127 ID=MS.gene057848.t1;Parent=MS.gene057848
chr4.4 exon 84379127 84380374 84379127 ID=MS.gene057848.t1.exon1;Parent=MS.gene057848.t1
chr4.4 CDS 84379127 84380374 84379127 ID=cds.MS.gene057848.t1;Parent=MS.gene057848.t1
Gene Sequence

>MS.gene057848

ATGATTTCACTTTCTAATGCTCTAAACAATGGTTTTAGTGTTGAACTCATCCACCGCGACTCTTCAAAATCACCACTCTACCAACCTACACAAAACAAATACCAACGTGTTACCAATGCCATGCGTCGTTCCATCAACCGCGTCAATAATTTTTACAAAGATTCCCTCACTAACACACCCAAATCCACTGTGATCCCAGCTAATGGTGAGTTTCTTATGACCTATTCAGTCGGTACTCCGCCATTTAAGTTATATGGTATTGTTGATACAGGTAGTGACATGGTTTGGCTTCAATCTAAGCCTTGTGAACATTGTTACAACCAAACGACTCCTATCTTTAATCCTTTGAAATCCTCAAGTTACAAAAACAATATTCCTTGTTTGTCTAAAAAATGTAAGTCTTCTAAATCTACCTTTTGTAGTAAAGATAAAACTTGCAAATATACTATTAAGTATGGTGATGGATCAATATCACAAGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACTGTGATAGGTTTTGGACATAAAAATACAGTGTCGTTTGAAGGCAATAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCAGTGTCACTTATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCAGAGTCGTCTAACTCGACGAGCAAACTCAGTTTCGGAGAAGCTGCTGTAGTTTCTGGTCATGGAGTTGTGTCAACACCTTTAGTGAAAAAAGATCCACAAATTTTTTACTATACAACATTGAAAGCATTTAGTGTTGGAAACAGAAGAGTAGAATTTTCAAATGGTAGTGTTGATGGTAACATCATGGTTGATTCAGGTACAACATTGACATTTGTACCATCTGGTATTTATCATAATTTAGAATCAATTGTGGTAAAATCGGTTAAACTGAAGCGCGCAAAGGATCCAACTGGTGGTATGTTTAACGTTTGTTATTCTGTTACATCAAATAAATATGATTTTCCTATAATCACTGCACATTTTGAAGGTGCAGATGTAAAGTTGCCTTCTATTGGCACCTTTGTTCCAGTTGCCGATGGGGTTGTTTGTTTAGCTCTTATCCCATCTCAACTCAATGAAGGCATCTTTGGGAATTTGGCTCAACAGAATTTGTTGGTTGGTTATGATCTTCAACAAAAAATCGTGTCTTTTAAGCCTACTGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene057848.t1

MISLSNALNNGFSVELIHRDSSKSPLYQPTQNKYQRVTNAMRRSINRVNNFYKDSLTNTPKSTVIPANGEFLMTYSVGTPPFKLYGIVDTGSDMVWLQSKPCEHCYNQTTPIFNPLKSSSYKNNIPCLSKKCKSSKSTFCSKDKTCKYTIKYGDGSISQGDLSLETLTLDSTSGSSISFPKTVIGFGHKNTVSFEGNSSGVVGLGNGPVSLINQLGSSIGGKFSYCLGPESSNSTSKLSFGEAAVVSGHGVVSTPLVKKDPQIFYYTTLKAFSVGNRRVEFSNGSVDGNIMVDSGTTLTFVPSGIYHNLESIVVKSVKLKRAKDPTGGMFNVCYSVTSNKYDFPIITAHFEGADVKLPSIGTFVPVADGVVCLALIPSQLNEGIFGNLAQQNLLVGYDLQQKIVSFKPTDCTKPY