AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057874

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057874.t1 MTR_4g073540 98.658 447 6 0 1 447 327 773 0.0 894
MS.gene057874.t1 MTR_8g022870 55.603 464 186 7 1 447 321 781 5.59e-165 484
MS.gene057874.t1 MTR_5g016120 52.135 445 204 6 1 442 323 761 6.09e-153 452
MS.gene057874.t1 MTR_4g095360 53.258 445 196 8 2 442 319 755 7.60e-153 452
MS.gene057874.t1 MTR_2g042130 52.116 449 205 7 2 447 337 778 2.23e-151 449
MS.gene057874.t1 MTR_4g125180 51.810 442 206 5 2 442 317 752 2.53e-151 448
MS.gene057874.t1 MTR_1g102350 51.106 452 209 6 1 447 338 782 6.48e-149 442
MS.gene057874.t1 MTR_3g102810 52.561 449 199 6 2 442 313 755 2.73e-146 435
MS.gene057874.t1 MTR_0047s0120 50.337 445 212 6 1 442 319 757 6.45e-146 434
MS.gene057874.t1 MTR_8g021130 51.910 445 191 9 5 439 341 772 2.07e-142 426
MS.gene057874.t1 MTR_1g018510 47.797 454 217 7 2 442 280 726 9.20e-131 394
MS.gene057874.t1 MTR_7g075900 46.309 447 225 8 2 442 335 772 8.03e-124 378
MS.gene057874.t1 MTR_4g102400 48.307 443 210 10 2 441 326 752 2.26e-118 363
MS.gene057874.t1 MTR_7g098630 47.903 453 213 11 1 441 319 760 4.30e-117 363
MS.gene057874.t1 MTR_3g082200 42.762 449 237 9 2 444 200 634 7.96e-117 355
MS.gene057874.t1 MTR_3g082200 42.762 449 237 9 2 444 215 649 1.89e-116 355
MS.gene057874.t1 MTR_4g134320 43.946 446 227 9 2 441 337 765 8.44e-116 357
MS.gene057874.t1 MTR_3g082200 42.762 449 237 9 2 444 338 772 1.16e-115 357
MS.gene057874.t1 MTR_5g055920 43.722 446 235 8 2 441 231 666 2.16e-112 345
MS.gene057874.t1 MTR_5g011340 44.989 449 220 10 2 441 320 750 2.13e-110 342
MS.gene057874.t1 MTR_5g011320 44.420 448 223 9 2 441 316 745 4.24e-110 342
MS.gene057874.t1 MTR_4g103480 43.274 446 237 9 2 441 351 786 1.06e-109 342
MS.gene057874.t1 MTR_5g011310 44.543 449 222 10 2 441 323 753 6.16e-109 338
MS.gene057874.t1 MTR_4g103450 43.049 446 238 9 2 441 308 743 1.69e-108 337
MS.gene057874.t1 MTR_7g098070 42.247 445 236 8 2 441 333 761 2.01e-108 338
MS.gene057874.t1 MTR_5g011190 44.543 449 222 11 2 441 323 753 3.09e-106 332
MS.gene057874.t1 MTR_4g103495 41.124 445 248 8 2 441 188 623 6.99e-106 327
MS.gene057874.t1 MTR_5g011370 44.321 449 223 11 2 441 343 773 2.99e-105 330
MS.gene057874.t1 MTR_4g103490 41.485 458 240 9 2 441 336 783 6.88e-104 326
MS.gene057874.t1 MTR_5g011260 43.177 447 229 11 2 441 323 751 1.35e-103 325
MS.gene057874.t1 MTR_4g103430 41.256 446 246 8 2 441 333 768 1.62e-103 325
MS.gene057874.t1 MTR_4g103400 42.013 457 238 13 2 443 334 778 2.83e-103 325
MS.gene057874.t1 MTR_5g011270 43.653 449 224 13 2 441 323 751 6.10e-103 323
MS.gene057874.t1 MTR_5g085690 41.485 458 238 13 2 441 343 788 1.49e-102 323
MS.gene057874.t1 MTR_3g061110 40.487 452 243 11 2 443 319 754 4.91e-102 321
MS.gene057874.t1 MTR_8g072440 40.980 449 229 10 2 441 289 710 1.16e-101 318
MS.gene057874.t1 MTR_8g072440 40.980 449 229 10 2 441 331 752 3.69e-101 318
MS.gene057874.t1 MTR_5g025010 42.316 449 225 10 2 441 294 717 1.09e-100 317
MS.gene057874.t1 MTR_5g025010 42.316 449 225 10 2 441 326 749 3.52e-100 316
MS.gene057874.t1 MTR_2g018135 42.000 450 239 9 2 442 347 783 4.34e-100 316
MS.gene057874.t1 MTR_8g024380 42.094 449 230 15 2 441 331 758 5.52e-98 310
MS.gene057874.t1 MTR_2g018125 42.094 449 239 9 2 442 349 784 1.49e-97 310
MS.gene057874.t1 MTR_1g078110 42.605 453 226 14 2 445 330 757 4.53e-97 308
MS.gene057874.t1 MTR_5g011280 41.295 448 237 11 2 441 326 755 4.64e-96 305
MS.gene057874.t1 MTR_5g096750 41.964 448 231 11 2 441 338 764 4.80e-96 305
MS.gene057874.t1 MTR_4g053630 39.778 450 247 9 2 444 223 655 1.26e-94 299
MS.gene057874.t1 MTR_4g053630 40.000 445 243 9 2 439 300 727 4.70e-94 299
MS.gene057874.t1 MTR_7g081750 38.936 470 243 12 2 441 342 797 4.78e-93 298
MS.gene057874.t1 MTR_2g049200 40.222 450 241 12 2 445 332 759 1.72e-92 296
MS.gene057874.t1 MTR_8g042670 39.912 456 244 13 2 445 199 636 3.60e-92 292
MS.gene057874.t1 MTR_4g103495 41.730 393 217 7 2 389 337 722 4.07e-92 295
MS.gene057874.t1 MTR_7g118080 41.814 452 231 13 2 444 330 758 2.11e-91 293
MS.gene057874.t1 MTR_8g024390 40.268 447 243 11 2 442 330 758 2.69e-91 293
MS.gene057874.t1 MTR_8g042670 39.912 456 244 13 2 445 323 760 3.90e-91 292
MS.gene057874.t1 MTR_8g024420 41.685 451 231 15 2 442 294 722 6.28e-91 291
MS.gene057874.t1 MTR_7g081420 40.393 458 243 14 2 443 343 786 2.69e-89 288
MS.gene057874.t1 MTR_4g053635 39.238 446 242 11 2 439 246 670 4.96e-89 285
MS.gene057874.t1 MTR_8g042710 39.073 453 248 11 2 441 326 763 6.53e-89 286
MS.gene057874.t1 MTR_7g081580 39.648 454 247 14 2 441 343 783 4.09e-87 283
MS.gene057874.t1 MTR_3g104930 38.562 459 247 14 2 442 321 762 1.56e-86 280
MS.gene057874.t1 MTR_7g081500 40.086 464 235 14 2 443 342 784 6.25e-86 279
MS.gene057874.t1 MTR_8g033150 39.073 453 233 15 2 441 302 724 2.41e-83 271
MS.gene057874.t1 MTR_8g033150 39.073 453 233 15 2 441 329 751 4.29e-83 271
MS.gene057874.t1 MTR_7g481600 39.341 455 247 13 2 441 343 783 2.26e-82 270
MS.gene057874.t1 MTR_8g083220 39.823 452 244 13 1 445 348 778 5.55e-82 268
MS.gene057874.t1 MTR_1g079120 39.374 447 249 14 2 441 329 760 7.12e-82 268
MS.gene057874.t1 MTR_7g081380 37.347 490 231 15 2 443 347 808 8.02e-82 269
MS.gene057874.t1 MTR_7g034380 38.514 444 245 12 2 441 272 691 1.36e-79 260
MS.gene057874.t1 MTR_8g024370 39.825 457 233 16 2 442 327 757 1.37e-78 259
MS.gene057874.t1 MTR_6g055570 37.528 453 241 14 2 444 312 732 1.72e-78 258
MS.gene057874.t1 MTR_8g079900 37.054 448 249 13 2 441 221 643 5.07e-78 255
MS.gene057874.t1 MTR_8g005710 37.004 454 244 12 2 444 279 701 1.04e-77 255
MS.gene057874.t1 MTR_8g079900 37.054 448 249 13 2 441 319 741 1.49e-77 256
MS.gene057874.t1 MTR_1g048110 37.584 447 246 14 2 441 310 730 9.67e-77 254
MS.gene057874.t1 MTR_3g464790 38.326 454 243 13 2 442 303 732 1.19e-76 253
MS.gene057874.t1 MTR_7g104200 38.987 454 237 12 1 441 315 741 6.55e-76 251
MS.gene057874.t1 MTR_7g075890 37.611 452 245 13 2 443 475 899 3.45e-75 252
MS.gene057874.t1 MTR_7g079310 36.980 457 246 17 2 441 332 763 4.56e-75 250
MS.gene057874.t1 MTR_7g079300 37.251 451 252 16 2 441 335 765 5.12e-75 250
MS.gene057874.t1 MTR_6g075370 37.055 421 182 10 2 413 318 664 7.72e-75 248
MS.gene057874.t1 MTR_3g114420 37.662 462 251 14 2 444 324 767 7.61e-74 246
MS.gene057874.t1 MTR_1g007030 36.242 447 252 11 2 441 312 732 7.81e-73 243
MS.gene057874.t1 MTR_1g006990 36.222 450 250 13 2 441 312 734 1.87e-72 242
MS.gene057874.t1 MTR_6g055410 34.889 450 239 15 2 444 312 714 1.36e-70 237
MS.gene057874.t1 MTR_8g006680 36.504 452 247 15 2 447 310 727 1.90e-69 234
MS.gene057874.t1 MTR_8g005300 36.504 452 247 15 2 447 310 727 1.90e-69 234
MS.gene057874.t1 MTR_8g005640 36.504 452 247 15 2 447 310 727 1.90e-69 234
MS.gene057874.t1 MTR_8g027260 42.122 311 162 6 136 444 360 654 3.26e-66 224
MS.gene057874.t1 MTR_3g010080 35.698 451 249 16 2 441 311 731 4.17e-66 225
MS.gene057874.t1 MTR_2g042650 34.513 452 241 13 2 444 240 645 1.12e-63 217
MS.gene057874.t1 MTR_2g044630 33.407 452 238 16 2 441 305 705 1.29e-53 191
MS.gene057874.t1 MTR_4g071560 40.520 269 141 7 1 256 259 521 4.19e-49 176
MS.gene057874.t1 MTR_4g127480 32.919 483 257 22 2 440 353 812 4.43e-49 179
MS.gene057874.t1 MTR_8g042710 41.573 267 138 6 2 256 326 586 1.56e-48 176
MS.gene057874.t1 MTR_2g037850 33.630 449 239 20 2 408 353 784 7.75e-47 173
MS.gene057874.t1 MTR_3g452290 30.717 446 216 15 2 444 216 571 1.25e-46 169
MS.gene057874.t1 MTR_3g451220 30.717 446 216 15 2 444 216 571 1.25e-46 169
MS.gene057874.t1 MTR_3g114410 39.231 260 147 5 2 254 325 580 2.85e-43 160
MS.gene057874.t1 MTR_2g042640 31.903 373 202 9 24 394 283 605 7.46e-42 157
MS.gene057874.t1 MTR_4g126230 37.727 220 125 4 147 365 40 248 1.25e-40 145
MS.gene057874.t1 MTR_3g114140 33.828 337 191 12 77 398 104 423 1.06e-38 145
MS.gene057874.t1 MTR_8g089230 29.792 480 268 21 9 441 388 845 1.74e-37 145
MS.gene057874.t1 MTR_2g046740 28.958 480 273 20 9 441 380 838 1.54e-35 140
MS.gene057874.t1 MTR_4g132620 26.829 492 270 17 2 436 351 809 1.36e-34 137
MS.gene057874.t1 MTR_5g044060 47.260 146 73 2 151 292 1 146 2.38e-34 127
MS.gene057874.t1 MTR_2g070260 38.208 212 111 7 223 431 1 195 2.68e-34 127
MS.gene057874.t1 MTR_4g053665 34.749 259 117 9 182 439 1 208 1.22e-26 107
MS.gene057874.t1 MTR_8g027660 35.484 217 102 8 234 445 67 250 5.89e-26 106
MS.gene057874.t1 MTR_7g079295 42.945 163 81 6 282 441 356 509 8.84e-26 110
MS.gene057874.t1 MTR_5g081130 37.427 171 98 5 272 441 10 172 2.44e-24 100
MS.gene057874.t1 MTR_4g118440 31.349 252 117 9 181 430 1 198 4.83e-24 100
MS.gene057874.t1 MTR_7g081660 38.421 190 105 6 253 441 374 552 4.72e-22 99.4
MS.gene057874.t1 MTR_7g097250 39.375 160 72 4 81 240 42 176 7.50e-22 92.8
MS.gene057874.t1 MTR_5g081100 35.450 189 92 6 2 183 261 426 7.35e-19 89.0
MS.gene057874.t1 MTR_5g011300 44.086 93 45 2 223 312 1 89 7.67e-16 73.2
MS.gene057874.t1 MTR_3g005755 44.086 93 45 3 197 282 214 306 5.24e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057874.t1 AT4G34980 74.165 449 113 3 1 447 317 764 0.0 681
MS.gene057874.t1 AT3G14240 56.710 462 181 8 1 447 318 775 2.14e-166 487
MS.gene057874.t1 AT5G67360 54.505 444 190 6 2 442 321 755 1.57e-161 474
MS.gene057874.t1 AT2G05920 52.489 442 201 7 2 442 317 750 2.95e-156 461
MS.gene057874.t1 AT5G51750 52.318 453 203 8 1 447 335 780 1.96e-151 449
MS.gene057874.t1 AT3G14067 49.441 447 212 8 2 441 328 767 3.68e-133 402
MS.gene057874.t1 AT5G59810 44.494 445 229 7 2 441 343 774 1.44e-120 369
MS.gene057874.t1 AT1G04110 44.766 449 232 8 2 442 331 771 8.20e-117 360
MS.gene057874.t1 AT2G04160 44.494 445 232 7 2 441 336 770 7.01e-113 349
MS.gene057874.t1 AT1G01900 43.973 448 235 9 2 442 334 772 8.66e-105 328
MS.gene057874.t1 AT1G20160 40.347 461 235 16 2 442 283 723 2.13e-91 292
MS.gene057874.t1 AT1G20160 40.347 461 235 16 2 442 322 762 6.06e-91 292
MS.gene057874.t1 AT5G45650 40.929 452 239 12 2 442 355 789 4.14e-90 290
MS.gene057874.t1 AT5G45650 40.929 452 239 12 2 442 355 789 4.14e-90 290
MS.gene057874.t1 AT5G59100 39.024 451 237 13 2 441 314 737 9.40e-86 278
MS.gene057874.t1 AT4G00230 39.911 451 233 13 2 441 320 743 6.43e-85 276
MS.gene057874.t1 AT5G59090 38.393 448 242 13 2 441 305 726 2.11e-83 271
MS.gene057874.t1 AT5G59090 38.393 448 242 13 2 441 307 728 2.15e-83 271
MS.gene057874.t1 AT5G59120 39.198 449 239 14 1 441 305 727 1.73e-82 269
MS.gene057874.t1 AT5G59090 38.170 448 238 13 2 441 307 723 1.84e-82 269
MS.gene057874.t1 AT3G46850 37.556 450 240 13 2 441 316 734 9.19e-81 264
MS.gene057874.t1 AT4G10520 39.821 447 241 13 1 444 327 748 2.87e-79 261
MS.gene057874.t1 AT5G59130 39.374 447 234 16 2 441 305 721 1.51e-78 258
MS.gene057874.t1 AT5G59130 39.374 447 234 16 2 441 309 725 1.66e-78 258
MS.gene057874.t1 AT5G59130 39.374 447 234 16 2 441 270 686 1.92e-78 257
MS.gene057874.t1 AT5G45640 39.490 471 222 15 2 444 319 754 2.63e-77 256
MS.gene057874.t1 AT1G32970 40.351 456 235 14 1 444 296 726 4.02e-77 254
MS.gene057874.t1 AT5G58840 38.288 444 223 13 2 441 310 706 1.96e-76 252
MS.gene057874.t1 AT3G46840 36.807 451 241 15 2 441 317 734 5.52e-75 249
MS.gene057874.t1 AT1G20150 38.793 464 247 16 1 442 323 771 7.11e-75 249
MS.gene057874.t1 AT4G26330 37.136 447 257 11 3 441 291 721 8.10e-75 248
MS.gene057874.t1 AT5G67090 36.574 432 233 13 2 430 322 715 8.69e-75 248
MS.gene057874.t1 AT4G26330 37.136 447 257 11 3 441 305 735 1.16e-74 248
MS.gene057874.t1 AT5G59130 38.926 447 226 17 2 441 309 715 2.41e-74 247
MS.gene057874.t1 AT5G58830 36.735 441 235 11 2 441 308 705 3.31e-74 246
MS.gene057874.t1 AT4G10550 38.326 454 247 13 1 444 284 714 4.06e-74 246
MS.gene057874.t1 AT4G10550 38.326 454 247 13 1 444 284 714 4.06e-74 246
MS.gene057874.t1 AT4G10550 38.326 454 247 13 1 444 340 770 1.11e-73 246
MS.gene057874.t1 AT4G10550 38.326 454 247 13 1 444 356 786 1.14e-73 246
MS.gene057874.t1 AT5G58820 36.591 440 236 12 2 441 304 700 2.79e-73 244
MS.gene057874.t1 AT4G21630 37.330 442 236 10 1 441 352 753 2.89e-73 245
MS.gene057874.t1 AT1G32950 38.998 459 238 15 1 444 283 714 3.12e-73 244
MS.gene057874.t1 AT1G32950 38.998 459 238 15 1 444 334 765 5.95e-73 244
MS.gene057874.t1 AT5G59190 35.841 452 256 11 2 446 269 693 1.26e-72 242
MS.gene057874.t1 AT5G11940 37.978 445 237 12 2 441 342 752 2.69e-72 242
MS.gene057874.t1 AT5G03620 38.758 467 234 16 1 441 318 758 7.33e-72 241
MS.gene057874.t1 AT4G10540 38.190 453 250 12 1 444 336 767 8.10e-72 241
MS.gene057874.t1 AT1G32940 38.852 453 247 13 1 444 284 715 9.96e-72 240
MS.gene057874.t1 AT1G32940 38.852 453 247 13 1 444 284 715 9.96e-72 240
MS.gene057874.t1 AT1G32940 38.852 453 247 13 1 444 335 766 2.33e-71 240
MS.gene057874.t1 AT4G21650 36.735 441 238 10 2 441 360 760 3.48e-71 239
MS.gene057874.t1 AT4G10530 38.341 446 234 14 2 444 332 739 6.66e-71 238
MS.gene057874.t1 AT4G10510 37.802 455 253 13 1 446 282 715 9.92e-71 237
MS.gene057874.t1 AT4G10510 37.802 455 253 13 1 446 267 700 1.05e-70 237
MS.gene057874.t1 AT4G10510 37.802 455 253 13 1 446 338 771 2.98e-70 237
MS.gene057874.t1 AT4G10510 37.802 455 253 13 1 446 326 759 3.67e-70 237
MS.gene057874.t1 AT1G32960 38.242 455 247 14 1 444 338 769 8.24e-70 236
MS.gene057874.t1 AT4G21640 35.520 442 216 11 1 441 354 727 3.21e-69 233
MS.gene057874.t1 AT4G21326 36.937 444 240 11 2 441 280 687 2.11e-63 217
MS.gene057874.t1 AT1G66220 33.634 443 253 10 2 441 340 744 2.07e-62 216
MS.gene057874.t1 AT1G66210 34.676 447 247 13 2 441 342 750 3.48e-61 212
MS.gene057874.t1 AT4G21323 35.068 442 249 12 2 439 384 791 3.85e-59 207
MS.gene057874.t1 AT4G15040 35.281 445 250 15 5 441 271 685 3.69e-58 203
MS.gene057874.t1 AT4G15040 35.281 445 250 15 5 441 271 685 3.69e-58 203
MS.gene057874.t1 AT4G00230 43.077 260 130 6 2 254 320 568 2.56e-52 185
MS.gene057874.t1 AT4G30020 33.038 451 240 21 2 408 350 782 2.25e-51 186
MS.gene057874.t1 AT4G30020 33.038 451 240 21 2 408 350 782 2.25e-51 186
MS.gene057874.t1 AT4G30020 33.038 451 240 21 2 408 350 782 2.25e-51 186
MS.gene057874.t1 AT4G30020 33.038 451 240 21 2 408 350 782 2.25e-51 186
MS.gene057874.t1 AT2G19170 32.091 483 262 23 2 440 349 809 1.63e-49 181
MS.gene057874.t1 AT2G19170 32.091 483 262 23 2 440 349 809 1.63e-49 181
MS.gene057874.t1 AT1G30600 31.405 484 270 20 2 441 361 826 2.97e-45 168
MS.gene057874.t1 AT2G39850 36.054 294 163 9 164 445 489 769 1.90e-44 166
MS.gene057874.t1 AT5G03620 41.418 268 133 7 1 254 318 575 3.98e-44 162
MS.gene057874.t1 AT5G44530 30.505 495 259 24 2 441 369 833 1.90e-41 157
MS.gene057874.t1 AT4G20430 29.980 497 262 23 2 442 383 849 1.27e-40 155
MS.gene057874.t1 AT4G20430 29.980 497 262 23 2 442 383 849 1.27e-40 155
MS.gene057874.t1 AT5G58840 37.154 253 128 6 2 254 310 531 5.86e-40 150
MS.gene057874.t1 AT1G32940 39.700 267 143 6 1 260 335 590 4.63e-39 149
MS.gene057874.t1 AT1G32980 44.211 190 99 4 219 407 3 186 5.98e-39 139
MS.gene057874.t1 AT5G44530 29.038 520 259 25 2 441 369 858 5.21e-37 144
MS.gene057874.t1 AT4G20430 29.508 488 252 22 2 442 383 825 1.11e-36 143
MS.gene057874.t1 AT1G62340 32.041 387 205 16 2 341 365 740 2.40e-35 139
MS.gene057874.t1 AT5G59110 41.212 165 90 5 279 441 7 166 2.53e-27 107
MS.gene057874.t1 AT1G20160 34.555 191 112 5 2 183 322 508 3.78e-23 102
MS.gene057874.t1 AT5G58840 31.319 182 94 6 2 183 310 460 1.85e-13 72.4

Find 148 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCCACACCTTATGATTT+TGG 0.100224 4.1:+30292838 MS.gene057874:CDS
GTCATTTGTGGCTATGTTTC+CGG 0.163880 4.1:+30293041 MS.gene057874:CDS
CGTCTTTGAAGCCACCTTTC+TGG 0.179824 4.1:-30293070 None:intergenic
AGGATCCATCTTCATGTTTC+GGG 0.250856 4.1:-30293253 None:intergenic
TGGGTCGGGTCATCTTAATT+TGG 0.256427 4.1:+30292858 MS.gene057874:CDS
GAATGTAGAGTCGCAGATTA+AGG 0.272187 4.1:+30293143 MS.gene057874:CDS
GTTAACTTTCTCTGTGCTAT+TGG 0.274028 4.1:+30292931 MS.gene057874:CDS
CCTGGAAAATCCGGTGTTCT+TGG 0.280590 4.1:+30292184 MS.gene057874:CDS
TTATGAACCTAAGGTTATTC+AGG 0.295037 4.1:+30292954 MS.gene057874:CDS
TACTCCGGTGTAGCTTTAAA+AGG 0.297949 4.1:+30292139 MS.gene057874:CDS
TTAGATCCAAAACAAATTAG+AGG 0.299862 4.1:+30292232 MS.gene057874:CDS
AGTTATGTTGTGACGGTAAC+TGG 0.308710 4.1:+30293225 MS.gene057874:CDS
AAGGATCCATCTTCATGTTT+CGG 0.332529 4.1:-30293254 None:intergenic
TGGCTTCAAAGACGTTTATA+AGG 0.343569 4.1:+30293079 MS.gene057874:CDS
TCTATTTGAGTAACGACAAT+TGG 0.344429 4.1:-30293333 None:intergenic
ATAAGGTGTGGAATTGTTTC+CGG 0.356241 4.1:-30292830 None:intergenic
GGGTCGGGTCATCTTAATTT+GGG 0.373429 4.1:+30292859 MS.gene057874:CDS
CTTCTTCCAGCTTGTGCCGT+TGG 0.402342 4.1:+30292388 MS.gene057874:CDS
TGAAATCTGCTCATCCTGAT+TGG 0.416082 4.1:+30292725 MS.gene057874:CDS
ACTGAGTTTAATATCTTGTC+TGG 0.416509 4.1:+30292658 MS.gene057874:CDS
TTGGGTAGAGCAATGGATCC+AGG 0.427766 4.1:+30292877 MS.gene057874:CDS
ACGGCGGCGGGACTCCAATC+AGG 0.428021 4.1:-30292739 None:intergenic
ATGTATCAATTAGTTTATCC+TGG 0.430481 4.1:+30292166 MS.gene057874:CDS
TATCTTGTCTGGTACTTCAA+TGG 0.434319 4.1:+30292669 MS.gene057874:CDS
TCTGATGCGGTGGGTCCTAC+TGG 0.438515 4.1:+30292613 MS.gene057874:CDS
TTAGTTTATCCTGGAAAATC+CGG 0.441931 4.1:+30292175 MS.gene057874:CDS
GAACCCACAGTTACTTAAAC+CGG 0.442171 4.1:+30292552 MS.gene057874:CDS
AAAATTGTAGTGTGTGATAG+AGG 0.445680 4.1:+30292256 MS.gene057874:CDS
GTAATTACCTGAATAACCTT+AGG 0.450890 4.1:-30292961 None:intergenic
CTATTTGAGTAACGACAATT+GGG 0.453789 4.1:-30293332 None:intergenic
TGCTATTGGTTATGAACCTA+AGG 0.461860 4.1:+30292945 MS.gene057874:CDS
AACATGAAGATGGATCCTTC+TGG 0.466297 4.1:+30293258 MS.gene057874:CDS
TCTAAATTACCCGTCATTTG+TGG 0.468575 4.1:+30293029 MS.gene057874:CDS
AATGGTGGCCGTTGGATTCG+CGG 0.469127 4.1:-30292452 None:intergenic
TTCCTCACCGGACATTTCTC+CGG 0.470623 4.1:-30292991 None:intergenic
TAACAGAAATCTTCCTATGT+TGG 0.476914 4.1:+30292798 MS.gene057874:CDS
TTTGATTATATCTCCTTCGT+TGG 0.478062 4.1:-30292413 None:intergenic
GATAGTTCCTTTGAAATCAA+TGG 0.481652 4.1:-30292470 None:intergenic
TTAAAGCTACACCGGAGTAG+AGG 0.482645 4.1:-30292135 None:intergenic
ATCTATCGTGCCTGCTCCGA+CGG 0.486038 4.1:-30292056 None:intergenic
ATCTCCTTCGTTGGAACCAA+CGG 0.486726 4.1:-30292404 None:intergenic
GACGGGTAATTTAGATTCTC+CGG 0.487326 4.1:-30293021 None:intergenic
AACTGTGGGTTCAAACCGTT+CGG 0.488374 4.1:-30292541 None:intergenic
AATGTAGAGTCGCAGATTAA+GGG 0.488768 4.1:+30293144 MS.gene057874:CDS
GCTGAAAACGAAGCTAGAAC+CGG 0.491124 4.1:-30292511 None:intergenic
AAACCGGATTTAATTGCTCC+GGG 0.491456 4.1:+30292568 MS.gene057874:CDS
ATGGCAGCACCGCATGTTAG+CGG 0.493865 4.1:+30292688 MS.gene057874:CDS
TTTCGGGTCGCTTACGTGGA+CGG 0.500840 4.1:+30293287 MS.gene057874:CDS
ATGGCGGATCTCACGGCGGC+GGG 0.503288 4.1:-30292751 None:intergenic
CCTCGGAATCAAACCCGCTC+CGG 0.504983 4.1:+30292492 MS.gene057874:CDS
TGCAGCTTGGTCTGATGCGG+TGG 0.505081 4.1:+30292603 MS.gene057874:CDS
GGGTTAGTTGTGAAGAAAGC+TGG 0.506504 4.1:+30292301 MS.gene057874:CDS
TCCGTGAAAACAAGCTTTGA+CGG 0.509066 4.1:-30293186 None:intergenic
TGGTTAACAACCGTCGGAGC+AGG 0.512671 4.1:+30292046 MS.gene057874:CDS
TCTTGGTGATTCACTTTGCA+TGG 0.512875 4.1:+30292201 MS.gene057874:CDS
GTGAAGAAAGCTGGTGGTGT+TGG 0.514596 4.1:+30292310 MS.gene057874:CDS
TAAACCGGATTTAATTGCTC+CGG 0.514768 4.1:+30292567 MS.gene057874:CDS
TTGAAATCAATGGTGGCCGT+TGG 0.523116 4.1:-30292460 None:intergenic
AACAGAAGCTGTGGTCATCA+TGG 0.525612 4.1:-30292770 None:intergenic
AACGAAGCTAGAACCGGAGC+GGG 0.528981 4.1:-30292505 None:intergenic
TCTTAATTTGGGTAGAGCAA+TGG 0.531154 4.1:+30292870 MS.gene057874:CDS
GTTTCCGGTGGGTTCCAGAA+AGG 0.535282 4.1:+30293056 MS.gene057874:CDS
CGGATGGTAAACATGTGGTT+CGG 0.536098 4.1:+30293307 MS.gene057874:CDS
ACTAACCCCTTCGCAACCCT+AGG 0.540691 4.1:-30292286 None:intergenic
GATTTCAAAGGAACTATCCT+CGG 0.541103 4.1:+30292475 MS.gene057874:CDS
TGACCCGACCCAAAATCATA+AGG 0.541807 4.1:-30292847 None:intergenic
TGTTAACCATGGAGCAAGGT+TGG 0.541934 4.1:-30292032 None:intergenic
ACCGCATGTTAGCGGTGCTG+CGG 0.542495 4.1:+30292696 MS.gene057874:CDS
TTGGAACCAACGGCACAAGC+TGG 0.542509 4.1:-30292394 None:intergenic
TCACGGAGGGTGTGAAGAAG+AGG 0.542952 4.1:+30293202 MS.gene057874:CDS
TCCGGTGGGTTCCAGAAAGG+TGG 0.545247 4.1:+30293059 MS.gene057874:CDS
CATGGCGGATCTCACGGCGG+CGG 0.546072 4.1:-30292752 None:intergenic
GGTCATCATGGCGGATCTCA+CGG 0.550946 4.1:-30292758 None:intergenic
CTCAAATAGAACCGTTGTAG+AGG 0.553053 4.1:+30293346 MS.gene057874:CDS
CTAGCAAACGGAATCTCAAA+CGG 0.553569 4.1:+30292340 MS.gene057874:CDS
GCAGCTTGGTCTGATGCGGT+GGG 0.554444 4.1:+30292604 MS.gene057874:CDS
AAATCTTCCTATGTTGGATG+AGG 0.555430 4.1:+30292804 MS.gene057874:CDS
ACGGCCACCATTGATTTCAA+AGG 0.559147 4.1:+30292463 MS.gene057874:CDS
TAAAGCTACACCGGAGTAGA+GGG 0.561787 4.1:-30292134 None:intergenic
AATCCGGTTTAAGTAACTGT+GGG 0.562369 4.1:-30292555 None:intergenic
TCGCCGATGATGATTTGTGC+TGG 0.562757 4.1:-30292088 None:intergenic
CCGGAGCGGGTTTGATTCCG+AGG 0.563635 4.1:-30292492 None:intergenic
GTTGGAATGATTCTAGCAAA+CGG 0.564865 4.1:+30292328 MS.gene057874:CDS
TCCGGTGAGGAAGTCTTCGC+CGG 0.564891 4.1:+30293002 MS.gene057874:CDS
TATAAGGACTGTGACTAATG+TGG 0.566485 4.1:+30293095 MS.gene057874:CDS
ATTTGTGGCTATGTTTCCGG+TGG 0.567254 4.1:+30293044 MS.gene057874:CDS
CCGGTAGCCTCATCCAACAT+AGG 0.569096 4.1:-30292811 None:intergenic
ATAAGGACTGTGACTAATGT+GGG 0.569412 4.1:+30293096 MS.gene057874:CDS
GAAACATAGCCACAAATGAC+GGG 0.570930 4.1:-30293038 None:intergenic
TCAGGTAATTACACGCAGAC+CGG 0.571070 4.1:+30292972 MS.gene057874:CDS
ACGCAGACCGGAGAAATGTC+CGG 0.573739 4.1:+30292984 MS.gene057874:CDS
GTGATAGAGGAAGTAGCCCT+AGG 0.575051 4.1:+30292269 MS.gene057874:CDS
AAGTAGCCCTAGGGTTGCGA+AGG 0.575626 4.1:+30292279 MS.gene057874:CDS
GCCGCAGCACCGCTAACATG+CGG 0.577119 4.1:-30292697 None:intergenic
GACTGTGACTAATGTGGGAC+CGG 0.577610 4.1:+30293101 MS.gene057874:CDS
CAGTAACCAACCTTGCTCCA+TGG 0.578395 4.1:+30292026 MS.gene057874:CDS
TGTCTTGTCCTCCTCTACAA+CGG 0.579472 4.1:-30293357 None:intergenic
TGGGTAGAGCAATGGATCCA+GGG 0.580170 4.1:+30292878 MS.gene057874:CDS
GGGTCGCTTACGTGGACGGA+TGG 0.580234 4.1:+30293291 MS.gene057874:CDS
TTTCCAGCACAAATCATCAT+CGG 0.581348 4.1:+30292085 MS.gene057874:CDS
AGATACCCGAAACATGAAGA+TGG 0.586476 4.1:+30293248 MS.gene057874:CDS
CCTATGTTGGATGAGGCTAC+CGG 0.587248 4.1:+30292811 MS.gene057874:CDS
CAGCTCAACCGCGAATCCAA+CGG 0.589762 4.1:+30292444 MS.gene057874:CDS
AACGGAATCTCAAACGGTGA+AGG 0.591986 4.1:+30292346 MS.gene057874:CDS
ACCGGATTTAATTGCTCCGG+GGG 0.594620 4.1:+30292570 MS.gene057874:CDS
GACCCGAAAACCGCTCCAGA+AGG 0.594953 4.1:-30293273 None:intergenic
TGTGCCGTTGGTTCCAACGA+AGG 0.597980 4.1:+30292400 MS.gene057874:CDS
GAAGAGGAGTTATGTTGTGA+CGG 0.600970 4.1:+30293218 MS.gene057874:CDS
GGAAACATAGCCACAAATGA+CGG 0.601092 4.1:-30293039 None:intergenic
CGGTTGTTAACCATGGAGCA+AGG 0.601403 4.1:-30292036 None:intergenic
TTTGTGGCTATGTTTCCGGT+GGG 0.602321 4.1:+30293045 MS.gene057874:CDS
AAATCCGGTTTAAGTAACTG+TGG 0.604563 4.1:-30292556 None:intergenic
CGACCCAAAATCATAAGGTG+TGG 0.605199 4.1:-30292842 None:intergenic
GGGTTAATATACTTGCAGCT+TGG 0.606913 4.1:+30292590 MS.gene057874:CDS
ATGTAGAGTCGCAGATTAAG+GGG 0.607484 4.1:+30293145 MS.gene057874:CDS
GGCGACGGAAGAAAATTCTC+CGG 0.608687 4.1:+30292106 MS.gene057874:CDS
TCCGGTGTATCCCTCTACTC+CGG 0.610598 4.1:+30292124 MS.gene057874:CDS
GTGATATCATAGACCAACCC+TGG 0.611253 4.1:-30292895 None:intergenic
CTTTGACGGCCTCACCGTCA+CGG 0.611700 4.1:-30293172 None:intergenic
GCTCCGACGGTTGTTAACCA+TGG 0.613364 4.1:-30292043 None:intergenic
TAGAGCAATGGATCCAGGGT+TGG 0.618776 4.1:+30292882 MS.gene057874:CDS
GCACAAATCATCATCGGCGA+CGG 0.618949 4.1:+30292091 MS.gene057874:CDS
TCCGGCGAAGACTTCCTCAC+CGG 0.623710 4.1:-30293003 None:intergenic
TTGGTCTATGATATCACTAA+CGG 0.626291 4.1:+30292901 MS.gene057874:CDS
TGATAGAGGAAGTAGCCCTA+GGG 0.628280 4.1:+30292270 MS.gene057874:CDS
ACACGATAAACTGAATTCAC+CGG 0.628656 4.1:-30293120 None:intergenic
GAAGATGGATCCTTCTGGAG+CGG 0.629122 4.1:+30293263 MS.gene057874:CDS
GCTCCATGGTTAACAACCGT+CGG 0.629415 4.1:+30292040 MS.gene057874:CDS
AGTAGCCCTAGGGTTGCGAA+GGG 0.633861 4.1:+30292280 MS.gene057874:CDS
GCTGCAAGTATATTAACCCC+CGG 0.640870 4.1:-30292586 None:intergenic
GTTATCAAGAACAGAAGCTG+TGG 0.644214 4.1:-30292779 None:intergenic
AGTTCCTTTGAAATCAATGG+TGG 0.644280 4.1:-30292467 None:intergenic
GCCACCTTTCTGGAACCCAC+CGG 0.645498 4.1:-30293060 None:intergenic
AAGTGAATCACCAAGAACAC+CGG 0.645808 4.1:-30292194 None:intergenic
GTATCAGAATCAAGACCAGT+AGG 0.663959 4.1:-30292628 None:intergenic
ACCGGAGTAGAGGGATACAC+CGG 0.664703 4.1:-30292125 None:intergenic
ACTTGCAGCTTGGTCTGATG+CGG 0.668489 4.1:+30292600 MS.gene057874:CDS
CTAACCCCTTCGCAACCCTA+GGG 0.669871 4.1:-30292285 None:intergenic
GACCGGAGAAATGTCCGGTG+AGG 0.674796 4.1:+30292989 MS.gene057874:CDS
AACCGGATTTAATTGCTCCG+GGG 0.682290 4.1:+30292569 MS.gene057874:CDS
TCAAACGGTGAAGGACTCGT+CGG 0.682506 4.1:+30292355 MS.gene057874:CDS
TGTAGAGTCGCAGATTAAGG+GGG 0.705690 4.1:+30293146 MS.gene057874:CDS
AAACGAAGCTAGAACCGGAG+CGG 0.716492 4.1:-30292506 None:intergenic
GTGGACGGATGGTAAACATG+TGG 0.716663 4.1:+30293302 MS.gene057874:CDS
AGAAGCTGTGGTCATCATGG+CGG 0.722272 4.1:-30292767 None:intergenic
TTAGTTGTGAAGAAAGCTGG+TGG 0.728582 4.1:+30292304 MS.gene057874:CDS
CATCATGGCGGATCTCACGG+CGG 0.748016 4.1:-30292755 None:intergenic
GTAGCCCTAGGGTTGCGAAG+GGG 0.777478 4.1:+30292281 MS.gene057874:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTCCTCTAATTTGTTT+TGG - chr4.1:30292241-30292260 None:intergenic 20.0%
! TTAGATCCAAAACAAATTAG+AGG + chr4.1:30292232-30292251 MS.gene057874:CDS 25.0%
!! ATGTATCAATTAGTTTATCC+TGG + chr4.1:30292166-30292185 MS.gene057874:CDS 25.0%
AAAATTGTAGTGTGTGATAG+AGG + chr4.1:30292256-30292275 MS.gene057874:CDS 30.0%
ACTGAGTTTAATATCTTGTC+TGG + chr4.1:30292658-30292677 MS.gene057874:CDS 30.0%
CTATTTGAGTAACGACAATT+GGG - chr4.1:30293335-30293354 None:intergenic 30.0%
GTAATTACCTGAATAACCTT+AGG - chr4.1:30292964-30292983 None:intergenic 30.0%
TAACAGAAATCTTCCTATGT+TGG + chr4.1:30292798-30292817 MS.gene057874:CDS 30.0%
TCTATTTGAGTAACGACAAT+TGG - chr4.1:30293336-30293355 None:intergenic 30.0%
TTAGTTTATCCTGGAAAATC+CGG + chr4.1:30292175-30292194 MS.gene057874:CDS 30.0%
TTATGAACCTAAGGTTATTC+AGG + chr4.1:30292954-30292973 MS.gene057874:CDS 30.0%
TTGGTCTATGATATCACTAA+CGG + chr4.1:30292901-30292920 MS.gene057874:CDS 30.0%
TTTGATTATATCTCCTTCGT+TGG - chr4.1:30292416-30292435 None:intergenic 30.0%
! AATTCCACACCTTATGATTT+TGG + chr4.1:30292838-30292857 MS.gene057874:CDS 30.0%
! ATTCCACACCTTATGATTTT+GGG + chr4.1:30292839-30292858 MS.gene057874:CDS 30.0%
!! GATAGTTCCTTTGAAATCAA+TGG - chr4.1:30292473-30292492 None:intergenic 30.0%
!!! TTTACCTTTTAAAGCTACAC+CGG - chr4.1:30292146-30292165 None:intergenic 30.0%
AAATCCGGTTTAAGTAACTG+TGG - chr4.1:30292559-30292578 None:intergenic 35.0%
AAATCTTCCTATGTTGGATG+AGG + chr4.1:30292804-30292823 MS.gene057874:CDS 35.0%
AAGGATCCATCTTCATGTTT+CGG - chr4.1:30293257-30293276 None:intergenic 35.0%
AATCCGGTTTAAGTAACTGT+GGG - chr4.1:30292558-30292577 None:intergenic 35.0%
AATGTAGAGTCGCAGATTAA+GGG + chr4.1:30293144-30293163 MS.gene057874:CDS 35.0%
ACACGATAAACTGAATTCAC+CGG - chr4.1:30293123-30293142 None:intergenic 35.0%
ATAAGGACTGTGACTAATGT+GGG + chr4.1:30293096-30293115 MS.gene057874:CDS 35.0%
ATAAGGTGTGGAATTGTTTC+CGG - chr4.1:30292833-30292852 None:intergenic 35.0%
GATTTCAAAGGAACTATCCT+CGG + chr4.1:30292475-30292494 MS.gene057874:CDS 35.0%
GTTAACTTTCTCTGTGCTAT+TGG + chr4.1:30292931-30292950 MS.gene057874:CDS 35.0%
GTTGGAATGATTCTAGCAAA+CGG + chr4.1:30292328-30292347 MS.gene057874:CDS 35.0%
TAAACCGGATTTAATTGCTC+CGG + chr4.1:30292567-30292586 MS.gene057874:CDS 35.0%
TATAAGGACTGTGACTAATG+TGG + chr4.1:30293095-30293114 MS.gene057874:CDS 35.0%
TATCTTGTCTGGTACTTCAA+TGG + chr4.1:30292669-30292688 MS.gene057874:CDS 35.0%
TCTAAATTACCCGTCATTTG+TGG + chr4.1:30293029-30293048 MS.gene057874:CDS 35.0%
TGGCTTCAAAGACGTTTATA+AGG + chr4.1:30293079-30293098 MS.gene057874:CDS 35.0%
TTTCCAGCACAAATCATCAT+CGG + chr4.1:30292085-30292104 MS.gene057874:CDS 35.0%
! TCTTAATTTGGGTAGAGCAA+TGG + chr4.1:30292870-30292889 MS.gene057874:CDS 35.0%
! TGCTATTGGTTATGAACCTA+AGG + chr4.1:30292945-30292964 MS.gene057874:CDS 35.0%
!! AGTTCCTTTGAAATCAATGG+TGG - chr4.1:30292470-30292489 None:intergenic 35.0%
AAACCGGATTTAATTGCTCC+GGG + chr4.1:30292568-30292587 MS.gene057874:CDS 40.0%
AACATGAAGATGGATCCTTC+TGG + chr4.1:30293258-30293277 MS.gene057874:CDS 40.0%
AAGTGAATCACCAAGAACAC+CGG - chr4.1:30292197-30292216 None:intergenic 40.0%
AGATACCCGAAACATGAAGA+TGG + chr4.1:30293248-30293267 MS.gene057874:CDS 40.0%
AGGATCCATCTTCATGTTTC+GGG - chr4.1:30293256-30293275 None:intergenic 40.0%
AGTTATGTTGTGACGGTAAC+TGG + chr4.1:30293225-30293244 MS.gene057874:CDS 40.0%
ATGTAGAGTCGCAGATTAAG+GGG + chr4.1:30293145-30293164 MS.gene057874:CDS 40.0%
CTAGCAAACGGAATCTCAAA+CGG + chr4.1:30292340-30292359 MS.gene057874:CDS 40.0%
GAAACATAGCCACAAATGAC+GGG - chr4.1:30293041-30293060 None:intergenic 40.0%
GAACCCACAGTTACTTAAAC+CGG + chr4.1:30292552-30292571 MS.gene057874:CDS 40.0%
GAAGAGGAGTTATGTTGTGA+CGG + chr4.1:30293218-30293237 MS.gene057874:CDS 40.0%
GAATGTAGAGTCGCAGATTA+AGG + chr4.1:30293143-30293162 MS.gene057874:CDS 40.0%
GACGGGTAATTTAGATTCTC+CGG - chr4.1:30293024-30293043 None:intergenic 40.0%
GGAAACATAGCCACAAATGA+CGG - chr4.1:30293042-30293061 None:intergenic 40.0%
GGGTTAATATACTTGCAGCT+TGG + chr4.1:30292590-30292609 MS.gene057874:CDS 40.0%
GTATCAGAATCAAGACCAGT+AGG - chr4.1:30292631-30292650 None:intergenic 40.0%
GTCATTTGTGGCTATGTTTC+CGG + chr4.1:30293041-30293060 MS.gene057874:CDS 40.0%
GTTATCAAGAACAGAAGCTG+TGG - chr4.1:30292782-30292801 None:intergenic 40.0%
TGAAATCTGCTCATCCTGAT+TGG + chr4.1:30292725-30292744 MS.gene057874:CDS 40.0%
TTAGTTGTGAAGAAAGCTGG+TGG + chr4.1:30292304-30292323 MS.gene057874:CDS 40.0%
! TCCGTGAAAACAAGCTTTGA+CGG - chr4.1:30293189-30293208 None:intergenic 40.0%
! TCTTGGTGATTCACTTTGCA+TGG + chr4.1:30292201-30292220 MS.gene057874:CDS 40.0%
!! ACACCTTATGATTTTGGGTC+GGG + chr4.1:30292844-30292863 MS.gene057874:CDS 40.0%
!! CACACCTTATGATTTTGGGT+CGG + chr4.1:30292843-30292862 MS.gene057874:CDS 40.0%
!! TACTCCGGTGTAGCTTTAAA+AGG + chr4.1:30292139-30292158 MS.gene057874:CDS 40.0%
!! TCAAAGCTTGTTTTCACGGA+GGG + chr4.1:30293189-30293208 MS.gene057874:CDS 40.0%
AACAGAAGCTGTGGTCATCA+TGG - chr4.1:30292773-30292792 None:intergenic 45.0%
AACCGGATTTAATTGCTCCG+GGG + chr4.1:30292569-30292588 MS.gene057874:CDS 45.0%
AACGGAATCTCAAACGGTGA+AGG + chr4.1:30292346-30292365 MS.gene057874:CDS 45.0%
AACTGTGGGTTCAAACCGTT+CGG - chr4.1:30292544-30292563 None:intergenic 45.0%
ATCTCCTTCGTTGGAACCAA+CGG - chr4.1:30292407-30292426 None:intergenic 45.0%
ATTTGTGGCTATGTTTCCGG+TGG + chr4.1:30293044-30293063 MS.gene057874:CDS 45.0%
CGACCCAAAATCATAAGGTG+TGG - chr4.1:30292845-30292864 None:intergenic 45.0%
CGGATGGTAAACATGTGGTT+CGG + chr4.1:30293307-30293326 MS.gene057874:CDS 45.0%
GCTGAAAACGAAGCTAGAAC+CGG - chr4.1:30292514-30292533 None:intergenic 45.0%
GCTGCAAGTATATTAACCCC+CGG - chr4.1:30292589-30292608 None:intergenic 45.0%
GGGTCGGGTCATCTTAATTT+GGG + chr4.1:30292859-30292878 MS.gene057874:CDS 45.0%
GTGATATCATAGACCAACCC+TGG - chr4.1:30292898-30292917 None:intergenic 45.0%
TAAAGCTACACCGGAGTAGA+GGG - chr4.1:30292137-30292156 None:intergenic 45.0%
TCAGGTAATTACACGCAGAC+CGG + chr4.1:30292972-30292991 MS.gene057874:CDS 45.0%
TGACCCGACCCAAAATCATA+AGG - chr4.1:30292850-30292869 None:intergenic 45.0%
TGATAGAGGAAGTAGCCCTA+GGG + chr4.1:30292270-30292289 MS.gene057874:CDS 45.0%
TGGGTCGGGTCATCTTAATT+TGG + chr4.1:30292858-30292877 MS.gene057874:CDS 45.0%
TGTAGAGTCGCAGATTAAGG+GGG + chr4.1:30293146-30293165 MS.gene057874:CDS 45.0%
TGTTAACCATGGAGCAAGGT+TGG - chr4.1:30292035-30292054 None:intergenic 45.0%
TTAAAGCTACACCGGAGTAG+AGG - chr4.1:30292138-30292157 None:intergenic 45.0%
TTGAAATCAATGGTGGCCGT+TGG - chr4.1:30292463-30292482 None:intergenic 45.0%
TTTGTGGCTATGTTTCCGGT+GGG + chr4.1:30293045-30293064 MS.gene057874:CDS 45.0%
! CTAGCTTCGTTTTCAGCTAG+AGG + chr4.1:30292517-30292536 MS.gene057874:CDS 45.0%
! TTTTCAGCTAGAGGACCGAA+CGG + chr4.1:30292526-30292545 MS.gene057874:CDS 45.0%
!! ACGGCCACCATTGATTTCAA+AGG + chr4.1:30292463-30292482 MS.gene057874:CDS 45.0%
!! GCCGTCAAAGCTTGTTTTCA+CGG + chr4.1:30293185-30293204 MS.gene057874:CDS 45.0%
!! GGGTTAGTTGTGAAGAAAGC+TGG + chr4.1:30292301-30292320 MS.gene057874:CDS 45.0%
!! GTCAAAGCTTGTTTTCACGG+AGG + chr4.1:30293188-30293207 MS.gene057874:CDS 45.0%
AAACGAAGCTAGAACCGGAG+CGG - chr4.1:30292509-30292528 None:intergenic 50.0%
ACCCCCGGAGCAATTAAATC+CGG - chr4.1:30292574-30292593 None:intergenic 50.0%
ACCGGATTTAATTGCTCCGG+GGG + chr4.1:30292570-30292589 MS.gene057874:CDS 50.0%
ACTTGCAGCTTGGTCTGATG+CGG + chr4.1:30292600-30292619 MS.gene057874:CDS 50.0%
AGAAGCTGTGGTCATCATGG+CGG - chr4.1:30292770-30292789 None:intergenic 50.0%
CAGTAACCAACCTTGCTCCA+TGG + chr4.1:30292026-30292045 MS.gene057874:CDS 50.0%
CCTATGTTGGATGAGGCTAC+CGG + chr4.1:30292811-30292830 MS.gene057874:CDS 50.0%
CCTGGAAAATCCGGTGTTCT+TGG + chr4.1:30292184-30292203 MS.gene057874:CDS 50.0%
CGGTTGTTAACCATGGAGCA+AGG - chr4.1:30292039-30292058 None:intergenic 50.0%
GACTGTGACTAATGTGGGAC+CGG + chr4.1:30293101-30293120 MS.gene057874:CDS 50.0%
GATTAAGGGGGTAACCGTGA+CGG + chr4.1:30293158-30293177 MS.gene057874:CDS 50.0%
GCACAAATCATCATCGGCGA+CGG + chr4.1:30292091-30292110 MS.gene057874:CDS 50.0%
GCTCCATGGTTAACAACCGT+CGG + chr4.1:30292040-30292059 MS.gene057874:CDS 50.0%
GGCGACGGAAGAAAATTCTC+CGG + chr4.1:30292106-30292125 MS.gene057874:CDS 50.0%
GTGATAGAGGAAGTAGCCCT+AGG + chr4.1:30292269-30292288 MS.gene057874:CDS 50.0%
GTGGACGGATGGTAAACATG+TGG + chr4.1:30293302-30293321 MS.gene057874:CDS 50.0%
TAGAGCAATGGATCCAGGGT+TGG + chr4.1:30292882-30292901 MS.gene057874:CDS 50.0%
TCAAACGGTGAAGGACTCGT+CGG + chr4.1:30292355-30292374 MS.gene057874:CDS 50.0%
TGGGTAGAGCAATGGATCCA+GGG + chr4.1:30292878-30292897 MS.gene057874:CDS 50.0%
TTCCTCACCGGACATTTCTC+CGG - chr4.1:30292994-30293013 None:intergenic 50.0%
TTGGGTAGAGCAATGGATCC+AGG + chr4.1:30292877-30292896 MS.gene057874:CDS 50.0%
! CCAAGAACACCGGATTTTCC+AGG - chr4.1:30292187-30292206 None:intergenic 50.0%
! GAAGATGGATCCTTCTGGAG+CGG + chr4.1:30293263-30293282 MS.gene057874:CDS 50.0%
! GTGAAGAAAGCTGGTGGTGT+TGG + chr4.1:30292310-30292329 MS.gene057874:CDS 50.0%
! TCGCCGATGATGATTTGTGC+TGG - chr4.1:30292091-30292110 None:intergenic 50.0%
!! CGTCTTTGAAGCCACCTTTC+TGG - chr4.1:30293073-30293092 None:intergenic 50.0%
!!! ATCCTTCTGGAGCGGTTTTC+GGG + chr4.1:30293271-30293290 MS.gene057874:CDS 50.0%
!!! GATCCTTCTGGAGCGGTTTT+CGG + chr4.1:30293270-30293289 MS.gene057874:CDS 50.0%
AACGAAGCTAGAACCGGAGC+GGG - chr4.1:30292508-30292527 None:intergenic 55.0%
AAGTAGCCCTAGGGTTGCGA+AGG + chr4.1:30292279-30292298 MS.gene057874:CDS 55.0%
AATGGTGGCCGTTGGATTCG+CGG - chr4.1:30292455-30292474 None:intergenic 55.0%
ACCGGAGTAGAGGGATACAC+CGG - chr4.1:30292128-30292147 None:intergenic 55.0%
ACGCAGACCGGAGAAATGTC+CGG + chr4.1:30292984-30293003 MS.gene057874:CDS 55.0%
ACTAACCCCTTCGCAACCCT+AGG - chr4.1:30292289-30292308 None:intergenic 55.0%
AGTAGCCCTAGGGTTGCGAA+GGG + chr4.1:30292280-30292299 MS.gene057874:CDS 55.0%
ATCTATCGTGCCTGCTCCGA+CGG - chr4.1:30292059-30292078 None:intergenic 55.0%
ATGGCAGCACCGCATGTTAG+CGG + chr4.1:30292688-30292707 MS.gene057874:CDS 55.0%
CAGCTCAACCGCGAATCCAA+CGG + chr4.1:30292444-30292463 MS.gene057874:CDS 55.0%
CCGGTAGCCTCATCCAACAT+AGG - chr4.1:30292814-30292833 None:intergenic 55.0%
CTAACCCCTTCGCAACCCTA+GGG - chr4.1:30292288-30292307 None:intergenic 55.0%
CTTCTTCCAGCTTGTGCCGT+TGG + chr4.1:30292388-30292407 MS.gene057874:CDS 55.0%
GCTCCGACGGTTGTTAACCA+TGG - chr4.1:30292046-30292065 None:intergenic 55.0%
TCCGGTGTATCCCTCTACTC+CGG + chr4.1:30292124-30292143 MS.gene057874:CDS 55.0%
TGGTTAACAACCGTCGGAGC+AGG + chr4.1:30292046-30292065 MS.gene057874:CDS 55.0%
TTGGAACCAACGGCACAAGC+TGG - chr4.1:30292397-30292416 None:intergenic 55.0%
! GTTTCCGGTGGGTTCCAGAA+AGG + chr4.1:30293056-30293075 MS.gene057874:CDS 55.0%
! TCACGGAGGGTGTGAAGAAG+AGG + chr4.1:30293202-30293221 MS.gene057874:CDS 55.0%
! TTTCGGGTCGCTTACGTGGA+CGG + chr4.1:30293287-30293306 MS.gene057874:CDS 55.0%
!! GGTCATCATGGCGGATCTCA+CGG - chr4.1:30292761-30292780 None:intergenic 55.0%
!! TGTGCCGTTGGTTCCAACGA+AGG + chr4.1:30292400-30292419 MS.gene057874:CDS 55.0%
CCTCGGAATCAAACCCGCTC+CGG + chr4.1:30292492-30292511 MS.gene057874:CDS 60.0%
CTTTGACGGCCTCACCGTCA+CGG - chr4.1:30293175-30293194 None:intergenic 60.0%
GACCCGAAAACCGCTCCAGA+AGG - chr4.1:30293276-30293295 None:intergenic 60.0%
GACCGGAGAAATGTCCGGTG+AGG + chr4.1:30292989-30293008 MS.gene057874:CDS 60.0%
GCAGCTTGGTCTGATGCGGT+GGG + chr4.1:30292604-30292623 MS.gene057874:CDS 60.0%
GTAGCCCTAGGGTTGCGAAG+GGG + chr4.1:30292281-30292300 MS.gene057874:CDS 60.0%
TCCGGCGAAGACTTCCTCAC+CGG - chr4.1:30293006-30293025 None:intergenic 60.0%
TCCGGTGAGGAAGTCTTCGC+CGG + chr4.1:30293002-30293021 MS.gene057874:CDS 60.0%
TCTGATGCGGTGGGTCCTAC+TGG + chr4.1:30292613-30292632 MS.gene057874:CDS 60.0%
TGCAGCTTGGTCTGATGCGG+TGG + chr4.1:30292603-30292622 MS.gene057874:CDS 60.0%
! ACCGCATGTTAGCGGTGCTG+CGG + chr4.1:30292696-30292715 MS.gene057874:CDS 60.0%
! TCCGGTGGGTTCCAGAAAGG+TGG + chr4.1:30293059-30293078 MS.gene057874:CDS 60.0%
!! CATCATGGCGGATCTCACGG+CGG - chr4.1:30292758-30292777 None:intergenic 60.0%
!! GCCACCTTTCTGGAACCCAC+CGG - chr4.1:30293063-30293082 None:intergenic 60.0%
!!! CGGTTTTCGGGTCGCTTACG+TGG + chr4.1:30293283-30293302 MS.gene057874:CDS 60.0%
AGGGGGTAACCGTGACGGTG+AGG + chr4.1:30293163-30293182 MS.gene057874:CDS 65.0%
GCCGCAGCACCGCTAACATG+CGG - chr4.1:30292700-30292719 None:intergenic 65.0%
GGGTCGCTTACGTGGACGGA+TGG + chr4.1:30293291-30293310 MS.gene057874:CDS 65.0%
! CCGGAGCGGGTTTGATTCCG+AGG - chr4.1:30292495-30292514 None:intergenic 65.0%
ACGGCGGCGGGACTCCAATC+AGG - chr4.1:30292742-30292761 None:intergenic 70.0%
!! ATGGCGGATCTCACGGCGGC+GGG - chr4.1:30292754-30292773 None:intergenic 70.0%
!! CATGGCGGATCTCACGGCGG+CGG - chr4.1:30292755-30292774 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 30292022 30293365 30292022 ID=MS.gene057874
chr4.1 mRNA 30292022 30293365 30292022 ID=MS.gene057874.t1;Parent=MS.gene057874
chr4.1 exon 30292022 30293365 30292022 ID=MS.gene057874.t1.exon1;Parent=MS.gene057874.t1
chr4.1 CDS 30292022 30293365 30292022 ID=cds.MS.gene057874.t1;Parent=MS.gene057874.t1
Gene Sequence

>MS.gene057874

ATGTCAGTAACCAACCTTGCTCCATGGTTAACAACCGTCGGAGCAGGCACGATAGATCGTGATTTTCCAGCACAAATCATCATCGGCGACGGAAGAAAATTCTCCGGTGTATCCCTCTACTCCGGTGTAGCTTTAAAAGGTAAAATGTATCAATTAGTTTATCCTGGAAAATCCGGTGTTCTTGGTGATTCACTTTGCATGGAAAATTCTTTAGATCCAAAACAAATTAGAGGAAAAATTGTAGTGTGTGATAGAGGAAGTAGCCCTAGGGTTGCGAAGGGGTTAGTTGTGAAGAAAGCTGGTGGTGTTGGAATGATTCTAGCAAACGGAATCTCAAACGGTGAAGGACTCGTCGGAGACGCTCATCTTCTTCCAGCTTGTGCCGTTGGTTCCAACGAAGGAGATATAATCAAAGCTTATATCAGCTCAACCGCGAATCCAACGGCCACCATTGATTTCAAAGGAACTATCCTCGGAATCAAACCCGCTCCGGTTCTAGCTTCGTTTTCAGCTAGAGGACCGAACGGTTTGAACCCACAGTTACTTAAACCGGATTTAATTGCTCCGGGGGTTAATATACTTGCAGCTTGGTCTGATGCGGTGGGTCCTACTGGTCTTGATTCTGATACACGTAGAACTGAGTTTAATATCTTGTCTGGTACTTCAATGGCAGCACCGCATGTTAGCGGTGCTGCGGCGTTGTTGAAATCTGCTCATCCTGATTGGAGTCCCGCCGCCGTGAGATCCGCCATGATGACCACAGCTTCTGTTCTTGATAACAGAAATCTTCCTATGTTGGATGAGGCTACCGGAAACAATTCCACACCTTATGATTTTGGGTCGGGTCATCTTAATTTGGGTAGAGCAATGGATCCAGGGTTGGTCTATGATATCACTAACGGTGACTATGTTAACTTTCTCTGTGCTATTGGTTATGAACCTAAGGTTATTCAGGTAATTACACGCAGACCGGAGAAATGTCCGGTGAGGAAGTCTTCGCCGGAGAATCTAAATTACCCGTCATTTGTGGCTATGTTTCCGGTGGGTTCCAGAAAGGTGGCTTCAAAGACGTTTATAAGGACTGTGACTAATGTGGGACCGGTGAATTCAGTTTATCGTGTGAATGTAGAGTCGCAGATTAAGGGGGTAACCGTGACGGTGAGGCCGTCAAAGCTTGTTTTCACGGAGGGTGTGAAGAAGAGGAGTTATGTTGTGACGGTAACTGGAGATACCCGAAACATGAAGATGGATCCTTCTGGAGCGGTTTTCGGGTCGCTTACGTGGACGGATGGTAAACATGTGGTTCGGAGCCCAATTGTCGTTACTCAAATAGAACCGTTGTAG
Protein sequence

>MS.gene057874.t1

MSVTNLAPWLTTVGAGTIDRDFPAQIIIGDGRKFSGVSLYSGVALKGKMYQLVYPGKSGVLGDSLCMENSLDPKQIRGKIVVCDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSNEGDIIKAYISSTANPTATIDFKGTILGIKPAPVLASFSARGPNGLNPQLLKPDLIAPGVNILAAWSDAVGPTGLDSDTRRTEFNILSGTSMAAPHVSGAAALLKSAHPDWSPAAVRSAMMTTASVLDNRNLPMLDEATGNNSTPYDFGSGHLNLGRAMDPGLVYDITNGDYVNFLCAIGYEPKVIQVITRRPEKCPVRKSSPENLNYPSFVAMFPVGSRKVASKTFIRTVTNVGPVNSVYRVNVESQIKGVTVTVRPSKLVFTEGVKKRSYVVTVTGDTRNMKMDPSGAVFGSLTWTDGKHVVRSPIVVTQIEPL