Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058028.t1 | RHN48529.1 | 96 | 100 | 4 | 0 | 1 | 100 | 1 | 100 | 2.40E-47 | 198.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058028.t1 | A0A396H5H6 | 96.0 | 100 | 4 | 0 | 1 | 100 | 1 | 100 | 1.7e-47 | 198.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058028 | MS.gene77220 | 0.819894 | 9.14E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAAATTTCTTGCCGAATT+TGG | 0.171145 | 4.2:-33831327 | None:intergenic |
| CGTTTCCACAATGGGAGTTT+TGG | 0.188134 | 4.2:-33831163 | None:intergenic |
| CGCCGTAGTTACGTCCATTC+AGG | 0.206652 | 4.2:-33831051 | None:intergenic |
| ACACAAAACTCAAGAGTCTT+TGG | 0.275344 | 4.2:+33831386 | MS.gene058028:CDS |
| AAGAGTCTTTGGGATGAATT+AGG | 0.280193 | 4.2:+33831397 | MS.gene058028:CDS |
| TTGCCGAATTTGGTAGATTC+TGG | 0.305141 | 4.2:-33831317 | None:intergenic |
| CACAAAACTCAAGAGTCTTT+GGG | 0.404850 | 4.2:+33831387 | MS.gene058028:CDS |
| CTGATACGGCAGCAGCAGTT+TGG | 0.405319 | 4.2:+33831257 | MS.gene058028:intron |
| AGTCCAGAATCTACCAAATT+CGG | 0.406350 | 4.2:+33831314 | MS.gene058028:CDS |
| CATTGCAACGTTTCCACAAT+GGG | 0.422099 | 4.2:-33831171 | None:intergenic |
| GCCTTTGCACCTTGCAATGT+TGG | 0.443618 | 4.2:-33830953 | None:intergenic |
| GCCGTAGTTACGTCCATTCA+GGG | 0.469541 | 4.2:-33831050 | None:intergenic |
| CTGGTTAACCAACCCCTGAA+TGG | 0.482462 | 4.2:+33831037 | MS.gene058028:CDS |
| TGGTGCAAGAGCAAAGGATC+AGG | 0.489714 | 4.2:-33830992 | None:intergenic |
| AGTTACGTCCATTCAGGGGT+TGG | 0.508176 | 4.2:-33831045 | None:intergenic |
| CTCCTTGAGAGAATCTATCA+TGG | 0.517191 | 4.2:-33831288 | None:intergenic |
| GTGGAAACGTTGCAATGATC+TGG | 0.518248 | 4.2:+33831177 | MS.gene058028:CDS |
| TGTAGTAATCAGAGATTGAG+AGG | 0.533983 | 4.2:-33831366 | None:intergenic |
| ACCAACATTGCAAGGTGCAA+AGG | 0.547629 | 4.2:+33830952 | MS.gene058028:CDS |
| CCCCTGAATGGACGTAACTA+CGG | 0.550213 | 4.2:+33831049 | MS.gene058028:CDS |
| AACAAACTCGGTCTGATCGA+TGG | 0.550559 | 4.2:+33831112 | MS.gene058028:CDS |
| GGCTGACAAAACCACTGCTA+CGG | 0.557763 | 4.2:+33830901 | MS.gene058028:CDS |
| TGGTTAACCAGTGATATCCC+TGG | 0.580153 | 4.2:-33831025 | None:intergenic |
| TTGCACCATTCTGACACACC+AGG | 0.581777 | 4.2:+33831007 | MS.gene058028:CDS |
| GAAATTTCTGAATGTCGTCA+AGG | 0.590019 | 4.2:+33831340 | MS.gene058028:CDS |
| ATGGACGTAACTACGGCGAA+TGG | 0.591740 | 4.2:+33831056 | MS.gene058028:CDS |
| CCGTAGTTACGTCCATTCAG+GGG | 0.597134 | 4.2:-33831049 | None:intergenic |
| TCAGAATGGTGCAAGAGCAA+AGG | 0.603114 | 4.2:-33830998 | None:intergenic |
| TCATTGCAACGTTTCCACAA+TGG | 0.606090 | 4.2:-33831172 | None:intergenic |
| TGGAACGGTGAAAGCGCCGT+CGG | 0.607927 | 4.2:+33831132 | MS.gene058028:CDS |
| ATATCCCTGGTGTGTCAGAA+TGG | 0.624380 | 4.2:-33831012 | None:intergenic |
| CTCCATGATAGATTCTCTCA+AGG | 0.625006 | 4.2:+33831286 | MS.gene058028:CDS |
| TGACACACCAGGGATATCAC+TGG | 0.627652 | 4.2:+33831018 | MS.gene058028:CDS |
| ACGACCCAAAACTCCCATTG+TGG | 0.653588 | 4.2:+33831158 | MS.gene058028:CDS |
| TGCACCATTCTGACACACCA+GGG | 0.667141 | 4.2:+33831008 | MS.gene058028:CDS |
| ACTCGGTCTGATCGATGGAA+CGG | 0.670633 | 4.2:+33831117 | MS.gene058028:CDS |
| GTAGTAATCAGAGATTGAGA+GGG | 0.707367 | 4.2:-33831365 | None:intergenic |
| CTTGAGAGAATCTATCATGG+AGG | 0.752829 | 4.2:-33831285 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAAAAACCAACATTGCA+AGG | + | chr4.2:33830944-33830963 | MS.gene058028:CDS | 30.0% | |
| ! | TAGCGTAATTTTCTCTGATA+CGG | + | chr4.2:33831243-33831262 | MS.gene058028:intron | 30.0% |
| ACACAAAACTCAAGAGTCTT+TGG | + | chr4.2:33831386-33831405 | MS.gene058028:CDS | 35.0% | |
| AGTCCAGAATCTACCAAATT+CGG | + | chr4.2:33831314-33831333 | MS.gene058028:CDS | 35.0% | |
| CACAAAACTCAAGAGTCTTT+GGG | + | chr4.2:33831387-33831406 | MS.gene058028:CDS | 35.0% | |
| CAGAAATTTCTTGCCGAATT+TGG | - | chr4.2:33831330-33831349 | None:intergenic | 35.0% | |
| GAAATTTCTGAATGTCGTCA+AGG | + | chr4.2:33831340-33831359 | MS.gene058028:CDS | 35.0% | |
| GTAGTAATCAGAGATTGAGA+GGG | - | chr4.2:33831368-33831387 | None:intergenic | 35.0% | |
| TGTAGTAATCAGAGATTGAG+AGG | - | chr4.2:33831369-33831388 | None:intergenic | 35.0% | |
| !! | GGTTTTTCTTTTGATTGCTC+TGG | - | chr4.2:33830935-33830954 | None:intergenic | 35.0% |
| AATTACGCTACGAGCTATGT+CGG | - | chr4.2:33831233-33831252 | None:intergenic | 40.0% | |
| CATTGCAACGTTTCCACAAT+GGG | - | chr4.2:33831174-33831193 | None:intergenic | 40.0% | |
| CTCCATGATAGATTCTCTCA+AGG | + | chr4.2:33831286-33831305 | MS.gene058028:CDS | 40.0% | |
| CTCCTTGAGAGAATCTATCA+TGG | - | chr4.2:33831291-33831310 | None:intergenic | 40.0% | |
| CTTGAGAGAATCTATCATGG+AGG | - | chr4.2:33831288-33831307 | None:intergenic | 40.0% | |
| TCATTGCAACGTTTCCACAA+TGG | - | chr4.2:33831175-33831194 | None:intergenic | 40.0% | |
| TTGCCGAATTTGGTAGATTC+TGG | - | chr4.2:33831320-33831339 | None:intergenic | 40.0% | |
| ! | GCAATGATCTGGTGTTAACA+TGG | + | chr4.2:33831188-33831207 | MS.gene058028:intron | 40.0% |
| ! | GTTTCCACAATGGGAGTTTT+GGG | - | chr4.2:33831165-33831184 | None:intergenic | 40.0% |
| ! | TGGATTTTTAACCGTAGCAG+TGG | - | chr4.2:33830915-33830934 | None:intergenic | 40.0% |
| !! | GATCAGACCGAGTTTGTTTT+TGG | - | chr4.2:33831110-33831129 | None:intergenic | 40.0% |
| AACAAACTCGGTCTGATCGA+TGG | + | chr4.2:33831112-33831131 | MS.gene058028:CDS | 45.0% | |
| ACCAACATTGCAAGGTGCAA+AGG | + | chr4.2:33830952-33830971 | MS.gene058028:CDS | 45.0% | |
| ATATCCCTGGTGTGTCAGAA+TGG | - | chr4.2:33831015-33831034 | None:intergenic | 45.0% | |
| ATTACGCTACGAGCTATGTC+GGG | - | chr4.2:33831232-33831251 | None:intergenic | 45.0% | |
| CTCAGCGCCAAAAACAAACT+CGG | + | chr4.2:33831100-33831119 | MS.gene058028:CDS | 45.0% | |
| GTGGAAACGTTGCAATGATC+TGG | + | chr4.2:33831177-33831196 | MS.gene058028:CDS | 45.0% | |
| TCAGAATGGTGCAAGAGCAA+AGG | - | chr4.2:33831001-33831020 | None:intergenic | 45.0% | |
| TGGTTAACCAGTGATATCCC+TGG | - | chr4.2:33831028-33831047 | None:intergenic | 45.0% | |
| ! | CGTTTCCACAATGGGAGTTT+TGG | - | chr4.2:33831166-33831185 | None:intergenic | 45.0% |
| ACGACCCAAAACTCCCATTG+TGG | + | chr4.2:33831158-33831177 | MS.gene058028:CDS | 50.0% | |
| ACTCGGTCTGATCGATGGAA+CGG | + | chr4.2:33831117-33831136 | MS.gene058028:CDS | 50.0% | |
| AGTTACGTCCATTCAGGGGT+TGG | - | chr4.2:33831048-33831067 | None:intergenic | 50.0% | |
| ATGGACGTAACTACGGCGAA+TGG | + | chr4.2:33831056-33831075 | MS.gene058028:CDS | 50.0% | |
| CCCCTGAATGGACGTAACTA+CGG | + | chr4.2:33831049-33831068 | MS.gene058028:CDS | 50.0% | |
| CCGTAGTTACGTCCATTCAG+GGG | - | chr4.2:33831052-33831071 | None:intergenic | 50.0% | |
| CTGGTTAACCAACCCCTGAA+TGG | + | chr4.2:33831037-33831056 | MS.gene058028:CDS | 50.0% | |
| GCCGTAGTTACGTCCATTCA+GGG | - | chr4.2:33831053-33831072 | None:intergenic | 50.0% | |
| GCCTTTGCACCTTGCAATGT+TGG | - | chr4.2:33830956-33830975 | None:intergenic | 50.0% | |
| GGCTGACAAAACCACTGCTA+CGG | + | chr4.2:33830901-33830920 | MS.gene058028:CDS | 50.0% | |
| TGACACACCAGGGATATCAC+TGG | + | chr4.2:33831018-33831037 | MS.gene058028:CDS | 50.0% | |
| TGCACCATTCTGACACACCA+GGG | + | chr4.2:33831008-33831027 | MS.gene058028:CDS | 50.0% | |
| TGGTGCAAGAGCAAAGGATC+AGG | - | chr4.2:33830995-33831014 | None:intergenic | 50.0% | |
| TGTCGGGTTCGATGGAATGA+AGG | - | chr4.2:33831216-33831235 | None:intergenic | 50.0% | |
| TTGCACCATTCTGACACACC+AGG | + | chr4.2:33831007-33831026 | MS.gene058028:CDS | 50.0% | |
| !!! | CGAGTTTGTTTTTGGCGCTG+AGG | - | chr4.2:33831102-33831121 | None:intergenic | 50.0% |
| ACGAGCTATGTCGGGTTCGA+TGG | - | chr4.2:33831224-33831243 | None:intergenic | 55.0% | |
| CGCCGTAGTTACGTCCATTC+AGG | - | chr4.2:33831054-33831073 | None:intergenic | 55.0% | |
| CTGATACGGCAGCAGCAGTT+TGG | + | chr4.2:33831257-33831276 | MS.gene058028:intron | 55.0% | |
| !!! | AGTTTTGGGTCGTCAGCCGA+CGG | - | chr4.2:33831151-33831170 | None:intergenic | 55.0% |
| !!! | TGTTTTTGGCGCTGAGGCTG+AGG | - | chr4.2:33831096-33831115 | None:intergenic | 55.0% |
| !! | TGGAACGGTGAAAGCGCCGT+CGG | + | chr4.2:33831132-33831151 | MS.gene058028:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 33830899 | 33831418 | 33830899 | ID=MS.gene058028 |
| chr4.2 | mRNA | 33830899 | 33831418 | 33830899 | ID=MS.gene058028.t1;Parent=MS.gene058028 |
| chr4.2 | exon | 33830899 | 33831198 | 33830899 | ID=MS.gene058028.t1.exon1;Parent=MS.gene058028.t1 |
| chr4.2 | CDS | 33830899 | 33831198 | 33830899 | ID=cds.MS.gene058028.t1;Parent=MS.gene058028.t1 |
| chr4.2 | exon | 33831269 | 33831418 | 33831269 | ID=MS.gene058028.t1.exon2;Parent=MS.gene058028.t1 |
| chr4.2 | CDS | 33831269 | 33831418 | 33831269 | ID=cds.MS.gene058028.t1;Parent=MS.gene058028.t1 |
| Gene Sequence |
| Protein sequence |
