AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77220.t1 AT3G42170 24.535 269 190 4 4 272 279 534 1.37e-24 104
MS.gene77220.t1 AT3G42170 24.535 269 190 4 4 272 257 512 1.41e-24 103

Find 65 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTCGTCATGCTGTTAAA+TGG 0.226041 7.2:-37349030 MS.gene77220:CDS
AATCAATAGCAAGATATTTA+AGG 0.228288 7.2:+37348556 None:intergenic
CTGACATCAATTTGTCCTTT+AGG 0.231615 7.2:+37348447 None:intergenic
TGGGACAATCACGTCCTAAA+TGG 0.268359 7.2:-37349142 MS.gene77220:CDS
AATTAAGTTCAATAAGTATT+GGG 0.303044 7.2:-37348591 MS.gene77220:CDS
TTAAACCTTATTGTGAAATC+TGG 0.306371 7.2:-37349085 MS.gene77220:CDS
TTCCGGTGCTTGCATATAAA+AGG 0.308832 7.2:+37348480 None:intergenic
TGCATAACATGATGGGTTCT+TGG 0.314314 7.2:-37349162 MS.gene77220:CDS
TGTGAACATAATCCCCATTT+AGG 0.321864 7.2:+37349128 None:intergenic
TCAAGCCAGATTTCACAATA+AGG 0.329442 7.2:+37349080 None:intergenic
AAATCCACAATTCGTTTGTC+TGG 0.342991 7.2:-37348731 MS.gene77220:CDS
TGACACTTATTTGAGGGATT+GGG 0.351965 7.2:-37349254 MS.gene77220:CDS
TTGACACTTATTTGAGGGAT+TGG 0.369677 7.2:-37349255 MS.gene77220:CDS
CAAATCCTCGCCATCTAGAT+TGG 0.370694 7.2:-37349005 MS.gene77220:CDS
TGTGACCAGTGCTTCGTATA+TGG 0.396163 7.2:-37348699 MS.gene77220:CDS
GGGACAATCACGTCCTAAAT+GGG 0.402021 7.2:-37349141 MS.gene77220:CDS
GCATAACATGATGGGTTCTT+GGG 0.406939 7.2:-37349161 MS.gene77220:CDS
ATGGAATAACAGATGAAGAT+TGG 0.418977 7.2:-37348796 MS.gene77220:CDS
GACACTTATTTGAGGGATTG+GGG 0.420586 7.2:-37349253 MS.gene77220:CDS
TATTGTGAAATCTGGCTTGA+AGG 0.426745 7.2:-37349077 MS.gene77220:CDS
TCGATATTTGCATAACATGA+TGG 0.431257 7.2:-37349170 MS.gene77220:CDS
GGTCACATTTCGTCGAGAAA+TGG 0.442539 7.2:-37348834 MS.gene77220:CDS
AAATTAAGTTCAATAAGTAT+TGG 0.447605 7.2:-37348592 MS.gene77220:CDS
GATTCGTCATGCTGTTAAAT+GGG 0.454005 7.2:-37349029 MS.gene77220:CDS
AAAGGCCTTTCTATACTTCA+AGG 0.455082 7.2:+37348877 None:intergenic
AGCAAGATATTTAAGGTGCC+TGG 0.463597 7.2:+37348563 None:intergenic
AATCATTACATCATTTGCCT+TGG 0.464135 7.2:+37349192 None:intergenic
TGGCAGAGGTGTTCACCATT+GGG 0.469417 7.2:-37348679 MS.gene77220:CDS
CGATATTTGCATAACATGAT+GGG 0.471153 7.2:-37349169 MS.gene77220:CDS
AATAAGTATTGGGGAGATCC+AGG 0.471916 7.2:-37348581 MS.gene77220:CDS
ATATGTTGACACTTATTTGA+GGG 0.475799 7.2:-37349260 MS.gene77220:CDS
ATGGCAGAGGTGTTCACCAT+TGG 0.479243 7.2:-37348680 MS.gene77220:CDS
AGTACAACAGCACCTTTGTA+TGG 0.483326 7.2:+37348947 None:intergenic
TGGAATAACAGATGAAGATT+GGG 0.483610 7.2:-37348795 MS.gene77220:CDS
CTCAATGTATTCAATGTTGA+CGG 0.491868 7.2:-37348903 MS.gene77220:CDS
AAGGGATCAACAAGCATATG+AGG 0.497638 7.2:-37348655 MS.gene77220:CDS
GTTGTATGGTAAGATATTAA+CGG 0.503435 7.2:-37349230 MS.gene77220:CDS
ATTAAGTTCAATAAGTATTG+GGG 0.531652 7.2:-37348590 MS.gene77220:CDS
AATATGTTGACACTTATTTG+AGG 0.532577 7.2:-37349261 MS.gene77220:CDS
GAGGTGTTCACCATTGGGAA+AGG 0.533854 7.2:-37348674 MS.gene77220:CDS
AGATCCAGACAAACGAATTG+TGG 0.535242 7.2:+37348727 None:intergenic
CTTGTTGATCCCTTTCCCAA+TGG 0.550514 7.2:+37348664 None:intergenic
AGGTGTTCACCATTGGGAAA+GGG 0.555935 7.2:-37348673 MS.gene77220:CDS
GCAAAATGCAGTTTCGCCCG+TGG 0.559044 7.2:+37348518 None:intergenic
ATCATGATGGGAGACATGTT+AGG 0.559259 7.2:-37349286 MS.gene77220:CDS
ATGGAAGAGAAAACAACGCA+TGG 0.563172 7.2:-37348815 MS.gene77220:CDS
TACTTGATGTTGACACTCGT+TGG 0.565341 7.2:-37348928 MS.gene77220:CDS
TTTATAAAACAACTCAAGCA+AGG 0.566250 7.2:+37348751 None:intergenic
CTCTGCCATATACGAAGCAC+TGG 0.569623 7.2:+37348694 None:intergenic
CAGTGCTTCGTATATGGCAG+AGG 0.574586 7.2:-37348693 MS.gene77220:CDS
ACACTTATTTGAGGGATTGG+GGG 0.584183 7.2:-37349252 MS.gene77220:CDS
GGTTGCCTTGAAGTATAGAA+AGG 0.587124 7.2:-37348882 MS.gene77220:CDS
TTCACGGGTCAAATCATGAT+GGG 0.595410 7.2:-37349298 None:intergenic
GAGTACAAATAAGTACATCA+TGG 0.599583 7.2:-37348633 MS.gene77220:CDS
TGTGAAATCTGGCTTGAAGG+AGG 0.610118 7.2:-37349074 MS.gene77220:CDS
TCGTACGCTTAACTTGTGAA+AGG 0.630292 7.2:+37348859 None:intergenic
TTATAAAACAACTCAAGCAA+GGG 0.634094 7.2:+37348752 None:intergenic
GATGAGAAAATTCCATACAA+AGG 0.635607 7.2:-37348959 MS.gene77220:CDS
TCACAAGTTAAGCGTACGAG+AGG 0.647053 7.2:-37348855 MS.gene77220:CDS
TAAGATATTAACGGTAACAG+TGG 0.653045 7.2:-37349221 MS.gene77220:CDS
GGACAATCACGTCCTAAATG+GGG 0.660028 7.2:-37349140 MS.gene77220:CDS
GGTAACAGTGGATAATGCCA+AGG 0.660938 7.2:-37349209 MS.gene77220:CDS
ATGGGGATTATGTTCACATG+CGG 0.678091 7.2:-37349123 MS.gene77220:CDS
GGCTTGAAGGAGGATAAACA+AGG 0.683086 7.2:-37349064 MS.gene77220:CDS
GCTTGAAGGAGGATAAACAA+GGG 0.733936 7.2:-37349063 MS.gene77220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAGTTCAATAAGTAT+TGG - chr7.2:37349088-37349107 MS.gene77220:CDS 15.0%
!! AATTAAGTTCAATAAGTATT+GGG - chr7.2:37349089-37349108 MS.gene77220:CDS 15.0%
!!! TTAGAAAAATTTGTTCAATA+TGG - chr7.2:37349259-37349278 MS.gene77220:CDS 15.0%
!! AATCAATAGCAAGATATTTA+AGG + chr7.2:37349127-37349146 None:intergenic 20.0%
!!! ATTAAGTTCAATAAGTATTG+GGG - chr7.2:37349090-37349109 MS.gene77220:CDS 20.0%
! AATATGTTGACACTTATTTG+AGG - chr7.2:37348419-37348438 MS.gene77220:CDS 25.0%
! ATATGTTGACACTTATTTGA+GGG - chr7.2:37348420-37348439 MS.gene77220:CDS 25.0%
! GTTGTATGGTAAGATATTAA+CGG - chr7.2:37348450-37348469 MS.gene77220:CDS 25.0%
! TTAAACCTTATTGTGAAATC+TGG - chr7.2:37348595-37348614 MS.gene77220:CDS 25.0%
! TTATAAAACAACTCAAGCAA+GGG + chr7.2:37348931-37348950 None:intergenic 25.0%
! TTTATAAAACAACTCAAGCA+AGG + chr7.2:37348932-37348951 None:intergenic 25.0%
!! GAAAAATTTGTTCAATATGG+TGG - chr7.2:37349262-37349281 MS.gene77220:CDS 25.0%
AATCATTACATCATTTGCCT+TGG + chr7.2:37348491-37348510 None:intergenic 30.0%
ATGGAATAACAGATGAAGAT+TGG - chr7.2:37348884-37348903 MS.gene77220:CDS 30.0%
CGATATTTGCATAACATGAT+GGG - chr7.2:37348511-37348530 MS.gene77220:CDS 30.0%
CTCAATGTATTCAATGTTGA+CGG - chr7.2:37348777-37348796 MS.gene77220:CDS 30.0%
GAGTACAAATAAGTACATCA+TGG - chr7.2:37349047-37349066 MS.gene77220:CDS 30.0%
GATGAGAAAATTCCATACAA+AGG - chr7.2:37348721-37348740 MS.gene77220:CDS 30.0%
TAAGATATTAACGGTAACAG+TGG - chr7.2:37348459-37348478 MS.gene77220:CDS 30.0%
TCGATATTTGCATAACATGA+TGG - chr7.2:37348510-37348529 MS.gene77220:CDS 30.0%
TGGAATAACAGATGAAGATT+GGG - chr7.2:37348885-37348904 MS.gene77220:CDS 30.0%
! ATGAACTTTTCCAATCTAGA+TGG + chr7.2:37348688-37348707 None:intergenic 30.0%
!!! CTTTAGGTGTTTAGTTTTTC+CGG + chr7.2:37349220-37349239 None:intergenic 30.0%
AAAGGCCTTTCTATACTTCA+AGG + chr7.2:37348806-37348825 None:intergenic 35.0%
AAATCCACAATTCGTTTGTC+TGG - chr7.2:37348949-37348968 MS.gene77220:CDS 35.0%
AGATTCGTCATGCTGTTAAA+TGG - chr7.2:37348650-37348669 MS.gene77220:CDS 35.0%
CGGAAAAACTAAACACCTAA+AGG - chr7.2:37349218-37349237 MS.gene77220:CDS 35.0%
CTGACATCAATTTGTCCTTT+AGG + chr7.2:37349236-37349255 None:intergenic 35.0%
GATTCGTCATGCTGTTAAAT+GGG - chr7.2:37348651-37348670 MS.gene77220:CDS 35.0%
TCAAGCCAGATTTCACAATA+AGG + chr7.2:37348603-37348622 None:intergenic 35.0%
TGACACTTATTTGAGGGATT+GGG - chr7.2:37348426-37348445 MS.gene77220:CDS 35.0%
TGTGAACATAATCCCCATTT+AGG + chr7.2:37348555-37348574 None:intergenic 35.0%
TTGACACTTATTTGAGGGAT+TGG - chr7.2:37348425-37348444 MS.gene77220:CDS 35.0%
! TATTGTGAAATCTGGCTTGA+AGG - chr7.2:37348603-37348622 MS.gene77220:CDS 35.0%
!! TTGCTTTTGTTCTTGATCCA+CGG - chr7.2:37349145-37349164 MS.gene77220:CDS 35.0%
AAGGGATCAACAAGCATATG+AGG - chr7.2:37349025-37349044 MS.gene77220:CDS 40.0%
ACACTTATTTGAGGGATTGG+GGG - chr7.2:37348428-37348447 MS.gene77220:CDS 40.0%
AGATCCAGACAAACGAATTG+TGG + chr7.2:37348956-37348975 None:intergenic 40.0%
AGTACAACAGCACCTTTGTA+TGG + chr7.2:37348736-37348755 None:intergenic 40.0%
ATGGAAGAGAAAACAACGCA+TGG - chr7.2:37348865-37348884 MS.gene77220:CDS 40.0%
ATGGGGATTATGTTCACATG+CGG - chr7.2:37348557-37348576 MS.gene77220:CDS 40.0%
GACACTTATTTGAGGGATTG+GGG - chr7.2:37348427-37348446 MS.gene77220:CDS 40.0%
GCTTGAAGGAGGATAAACAA+GGG - chr7.2:37348617-37348636 MS.gene77220:CDS 40.0%
GGTTGCCTTGAAGTATAGAA+AGG - chr7.2:37348798-37348817 MS.gene77220:CDS 40.0%
TACTTGATGTTGACACTCGT+TGG - chr7.2:37348752-37348771 MS.gene77220:CDS 40.0%
TTCCGGTGCTTGCATATAAA+AGG + chr7.2:37349203-37349222 None:intergenic 40.0%
! AGCAAGATATTTAAGGTGCC+TGG + chr7.2:37349120-37349139 None:intergenic 40.0%
! GACCTTTTATATGCAAGCAC+CGG - chr7.2:37349198-37349217 MS.gene77220:CDS 40.0%
! TGCTTTTGTTCTTGATCCAC+GGG - chr7.2:37349146-37349165 MS.gene77220:CDS 40.0%
!! AATAAGTATTGGGGAGATCC+AGG - chr7.2:37349099-37349118 MS.gene77220:CDS 40.0%
!! GCATAACATGATGGGTTCTT+GGG - chr7.2:37348519-37348538 MS.gene77220:CDS 40.0%
!! TCGTACGCTTAACTTGTGAA+AGG + chr7.2:37348824-37348843 None:intergenic 40.0%
!! TGCATAACATGATGGGTTCT+TGG - chr7.2:37348518-37348537 MS.gene77220:CDS 40.0%
AGGTGTTCACCATTGGGAAA+GGG - chr7.2:37349007-37349026 MS.gene77220:CDS 45.0%
CAAATCCTCGCCATCTAGAT+TGG - chr7.2:37348675-37348694 MS.gene77220:CDS 45.0%
CTTGTTGATCCCTTTCCCAA+TGG + chr7.2:37349019-37349038 None:intergenic 45.0%
GGACAATCACGTCCTAAATG+GGG - chr7.2:37348540-37348559 MS.gene77220:CDS 45.0%
GGCTTGAAGGAGGATAAACA+AGG - chr7.2:37348616-37348635 MS.gene77220:CDS 45.0%
GGGACAATCACGTCCTAAAT+GGG - chr7.2:37348539-37348558 MS.gene77220:CDS 45.0%
GGTAACAGTGGATAATGCCA+AGG - chr7.2:37348471-37348490 MS.gene77220:CDS 45.0%
GGTCACATTTCGTCGAGAAA+TGG - chr7.2:37348846-37348865 MS.gene77220:CDS 45.0%
TCACAAGTTAAGCGTACGAG+AGG - chr7.2:37348825-37348844 MS.gene77220:CDS 45.0%
TGGGACAATCACGTCCTAAA+TGG - chr7.2:37348538-37348557 MS.gene77220:CDS 45.0%
TGTGACCAGTGCTTCGTATA+TGG - chr7.2:37348981-37349000 MS.gene77220:CDS 45.0%
! CTTTTCCAATCTAGATGGCG+AGG + chr7.2:37348683-37348702 None:intergenic 45.0%
! TGTGAAATCTGGCTTGAAGG+AGG - chr7.2:37348606-37348625 MS.gene77220:CDS 45.0%
CAGTGCTTCGTATATGGCAG+AGG - chr7.2:37348987-37349006 MS.gene77220:CDS 50.0%
CTCTGCCATATACGAAGCAC+TGG + chr7.2:37348989-37349008 None:intergenic 50.0%
TTGAGGGATTGGGGGTTGTA+TGG - chr7.2:37348436-37348455 MS.gene77220:CDS 50.0%
! GAGGTGTTCACCATTGGGAA+AGG - chr7.2:37349006-37349025 MS.gene77220:CDS 50.0%
!! ATGGCAGAGGTGTTCACCAT+TGG - chr7.2:37349000-37349019 MS.gene77220:CDS 50.0%
!! TGGCAGAGGTGTTCACCATT+GGG - chr7.2:37349001-37349020 MS.gene77220:CDS 50.0%
GCAAAATGCAGTTTCGCCCG+TGG + chr7.2:37349165-37349184 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 37348397 37349305 37348397 ID=MS.gene77220
chr7.2 mRNA 37348397 37349305 37348397 ID=MS.gene77220.t1;Parent=MS.gene77220
chr7.2 exon 37348397 37349305 37348397 ID=MS.gene77220.t1.exon1;Parent=MS.gene77220.t1
chr7.2 CDS 37348397 37349305 37348397 ID=cds.MS.gene77220.t1;Parent=MS.gene77220.t1
Gene Sequence

>MS.gene77220

ATGATGGGAGACATGTTAGGTAAATATGTTGACACTTATTTGAGGGATTGGGGGTTGTATGGTAAGATATTAACGGTAACAGTGGATAATGCCAAGGCAAATGATGTAATGATTCGATATTTGCATAACATGATGGGTTCTTGGGACAATCACGTCCTAAATGGGGATTATGTTCACATGCGGTGTTGCACGCATGTATTAAACCTTATTGTGAAATCTGGCTTGAAGGAGGATAAACAAGGGTCTATTTCAAAGATTCGTCATGCTGTTAAATGGGTCAAATCCTCGCCATCTAGATTGGAAAAGTTCATGATATGTGCTTCTGATGAGAAAATTCCATACAAAGGTGCTGTTGTACTTGATGTTGACACTCGTTGGAACTCAATGTATTCAATGTTGACGGTTGCCTTGAAGTATAGAAAGGCCTTTCACAAGTTAAGCGTACGAGAGGTCACATTTCGTCGAGAAATGGAAGAGAAAACAACGCATGGAATAACAGATGAAGATTGGGACTATGTTGAATCAGTGCTTCCCTTGCTTGAGTTGTTTTATAAATCCACAATTCGTTTGTCTGGATCTTTGTATGTGACCAGTGCTTCGTATATGGCAGAGGTGTTCACCATTGGGAAAGGGATCAACAAGCATATGAGGAGTACAAATAAGTACATCATGGAGACAGCAAACAAGATGAAAATTAAGTTCAATAAGTATTGGGGAGATCCAGGCACCTTAAATATCTTGCTATTGATTGCTTTTGTTCTTGATCCACGGGCGAAACTGCATTTTGCTGAATTTTATATTGACCTTTTATATGCAAGCACCGGAAAAACTAAACACCTAAAGGACAAATTGATGTCAGATTTTAGAAAAATTTGTTCAATATGGTGGTACAGATTACTCTCAATCTAG

Protein sequence

>MS.gene77220.t1

MMGDMLGKYVDTYLRDWGLYGKILTVTVDNAKANDVMIRYLHNMMGSWDNHVLNGDYVHMRCCTHVLNLIVKSGLKEDKQGSISKIRHAVKWVKSSPSRLEKFMICASDEKIPYKGAVVLDVDTRWNSMYSMLTVALKYRKAFHKLSVREVTFRREMEEKTTHGITDEDWDYVESVLPLLELFYKSTIRLSGSLYVTSASYMAEVFTIGKGINKHMRSTNKYIMETANKMKIKFNKYWGDPGTLNILLLIAFVLDPRAKLHFAEFYIDLLYASTGKTKHLKDKLMSDFRKICSIWWYRLLSI