Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058164.t1 | AES62102.2 | 90.2 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 2.60E-08 | 67.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058164.t1 | G7I4Y9 | 90.2 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 1.9e-08 | 67.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058164 | MS.gene09574 | 0.805316 | 1.42E-49 | -1.69E-46 |
| MS.gene058164 | MS.gene23009 | 0.823709 | 1.20E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058164.t1 | MTR_1g093960 | 96.721 | 61 | 2 | 0 | 1 | 61 | 1 | 61 | 2.69e-33 | 108 |
| MS.gene058164.t1 | MTR_1g093985 | 96.721 | 61 | 2 | 0 | 1 | 61 | 1 | 61 | 2.69e-33 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAGGAGTAGCTTCTTTCT+TGG | 0.263984 | 1.4:-73547612 | None:intergenic |
| AGCTTTCTTTGAAGCTGATT+TGG | 0.275824 | 1.4:-73547657 | None:intergenic |
| TGACCCTTATCACTAGCTTT+AGG | 0.299140 | 1.4:-73547629 | None:intergenic |
| TCAAGATCTTAATAAGAAAA+AGG | 0.310643 | 1.4:+73547691 | MS.gene058164:CDS |
| TACTCCTAAAGCTAGTGATA+AGG | 0.421532 | 1.4:+73547625 | MS.gene058164:CDS |
| ACTCCTAAAGCTAGTGATAA+GGG | 0.443378 | 1.4:+73547626 | MS.gene058164:CDS |
| AACAGGTTTATCAGCCTTCA+AGG | 0.447580 | 1.4:-73547567 | None:intergenic |
| GTGCAAAATGGCATCCTTGA+AGG | 0.478600 | 1.4:+73547553 | None:intergenic |
| CTTGGTTGTCTACAGGGAAA+AGG | 0.487214 | 1.4:+73547837 | MS.gene058164:intron |
| TTGAAGGCTGATAAACCTGT+TGG | 0.591373 | 1.4:+73547569 | MS.gene058164:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGCACATATATAGTAATA+GGG | - | chr1.4:73547792-73547811 | None:intergenic | 20.0% |
| !! | TCAAGATCTTAATAAGAAAA+AGG | + | chr1.4:73547691-73547710 | MS.gene058164:CDS | 20.0% |
| !!! | TCAACTTTTTTTTTTATCCT+TGG | + | chr1.4:73547819-73547838 | MS.gene058164:intron | 20.0% |
| !!! | TTTTAATCTTGCTTATATGA+TGG | + | chr1.4:73547744-73547763 | MS.gene058164:intron | 20.0% |
| !!! | TTTTTCTTATTAAGATCTTG+AGG | - | chr1.4:73547692-73547711 | None:intergenic | 20.0% |
| ! | GTAAGCACATATATAGTAAT+AGG | - | chr1.4:73547793-73547812 | None:intergenic | 25.0% |
| TAATCTTGCTTATATGATGG+TGG | + | chr1.4:73547747-73547766 | MS.gene058164:intron | 30.0% | |
| ACTCCTAAAGCTAGTGATAA+GGG | + | chr1.4:73547626-73547645 | MS.gene058164:CDS | 35.0% | |
| TACTCCTAAAGCTAGTGATA+AGG | + | chr1.4:73547625-73547644 | MS.gene058164:CDS | 35.0% | |
| TTTAGGAGTAGCTTCTTTCT+TGG | - | chr1.4:73547615-73547634 | None:intergenic | 35.0% | |
| TTTATCCTTGGTTGTCTACA+GGG | + | chr1.4:73547831-73547850 | MS.gene058164:intron | 35.0% | |
| ! | TTTTATCCTTGGTTGTCTAC+AGG | + | chr1.4:73547830-73547849 | MS.gene058164:intron | 35.0% |
| !! | AGCTTTCTTTGAAGCTGATT+TGG | - | chr1.4:73547660-73547679 | None:intergenic | 35.0% |
| AACAGGTTTATCAGCCTTCA+AGG | - | chr1.4:73547570-73547589 | None:intergenic | 40.0% | |
| TTGAAGGCTGATAAACCTGT+TGG | + | chr1.4:73547569-73547588 | MS.gene058164:CDS | 40.0% | |
| !! | TGACCCTTATCACTAGCTTT+AGG | - | chr1.4:73547632-73547651 | None:intergenic | 40.0% |
| CTTGGTTGTCTACAGGGAAA+AGG | + | chr1.4:73547837-73547856 | MS.gene058164:intron | 45.0% | |
| ! | CTGTTGGCACCCAACTTTTT+GGG | + | chr1.4:73547585-73547604 | MS.gene058164:CDS | 45.0% |
| ! | CTTTTCCCTGTAGACAACCA+AGG | - | chr1.4:73547839-73547858 | None:intergenic | 45.0% |
| CCAAAAAGTTGGGTGCCAAC+AGG | - | chr1.4:73547587-73547606 | None:intergenic | 50.0% | |
| CTTGGCCTGCCCAAAAAGTT+GGG | - | chr1.4:73547597-73547616 | None:intergenic | 50.0% | |
| TCTTGGCCTGCCCAAAAAGT+TGG | - | chr1.4:73547598-73547617 | None:intergenic | 50.0% | |
| ! | CCTGTTGGCACCCAACTTTT+TGG | + | chr1.4:73547584-73547603 | MS.gene058164:CDS | 50.0% |
| !! | TGGCACCCAACTTTTTGGGC+AGG | + | chr1.4:73547589-73547608 | MS.gene058164:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 73547560 | 73547884 | 73547560 | ID=MS.gene058164 |
| chr1.4 | mRNA | 73547560 | 73547884 | 73547560 | ID=MS.gene058164.t1;Parent=MS.gene058164 |
| chr1.4 | exon | 73547560 | 73547712 | 73547560 | ID=MS.gene058164.t1.exon1;Parent=MS.gene058164.t1 |
| chr1.4 | CDS | 73547560 | 73547712 | 73547560 | ID=cds.MS.gene058164.t1;Parent=MS.gene058164.t1 |
| chr1.4 | exon | 73547852 | 73547884 | 73547852 | ID=MS.gene058164.t1.exon2;Parent=MS.gene058164.t1 |
| chr1.4 | CDS | 73547852 | 73547884 | 73547852 | ID=cds.MS.gene058164.t1;Parent=MS.gene058164.t1 |
| Gene Sequence |
| Protein sequence |
