AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23009


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23009.t1 MTR_7g108200 99.020 102 1 0 1 102 1 102 1.51e-72 210
MS.gene23009.t1 MTR_1g088920 78.571 98 21 0 1 98 1 98 2.48e-57 172
MS.gene23009.t1 MTR_1g088925 76.531 98 23 0 1 98 1 98 3.56e-57 171
MS.gene23009.t1 MTR_1g088910 57.447 94 40 0 1 94 1 94 2.57e-37 121
MS.gene23009.t1 MTR_2g019900 55.789 95 42 0 1 95 1 95 3.13e-37 121
MS.gene23009.t1 MTR_2g019950 55.789 95 42 0 1 95 1 95 5.90e-37 120
MS.gene23009.t1 MTR_7g108220 53.684 95 43 1 1 94 1 95 1.54e-32 109
MS.gene23009.t1 MTR_7g022690 46.875 96 50 1 1 95 30 125 3.84e-32 109
MS.gene23009.t1 MTR_7g108210 52.632 95 44 1 1 94 1 95 1.05e-31 107
MS.gene23009.t1 MTR_7g022710 46.875 96 50 1 1 95 30 125 1.05e-31 108
MS.gene23009.t1 MTR_4g119050 50.000 96 47 1 1 95 1 96 1.75e-31 107
MS.gene23009.t1 MTR_4g119030 50.000 96 47 1 1 95 1 96 1.75e-31 107
MS.gene23009.t1 MTR_4g119040 50.000 96 47 1 1 95 1 96 1.75e-31 107
MS.gene23009.t1 MTR_7g022550 45.833 96 51 1 1 95 30 125 6.78e-31 106
MS.gene23009.t1 MTR_7g026770 50.000 96 47 1 1 95 22 117 1.34e-30 110
MS.gene23009.t1 MTR_7g108250 45.263 95 52 0 1 95 1 95 3.91e-26 93.6
MS.gene23009.t1 MTR_1g088905 42.553 94 54 0 1 94 1 94 1.02e-24 89.7
MS.gene23009.t1 MTR_1g088895 44.681 94 52 0 1 94 1 94 6.68e-22 82.8
MS.gene23009.t1 MTR_7g108260 42.553 94 54 0 1 94 1 94 1.67e-21 81.6
MS.gene23009.t1 MTR_2g014760 38.298 94 58 0 2 95 27 120 8.30e-19 75.5
MS.gene23009.t1 MTR_2g048970 38.776 98 56 1 2 95 29 126 1.21e-17 72.8
MS.gene23009.t1 MTR_2g090755 34.375 96 56 2 4 95 41 133 1.75e-16 70.1
MS.gene23009.t1 MTR_1g069255 36.170 94 59 1 2 94 84 177 1.58e-15 68.6
MS.gene23009.t1 MTR_4g079110 36.082 97 59 2 2 95 52 148 9.10e-15 65.9
MS.gene23009.t1 MTR_3g077570 32.979 94 63 0 1 94 18 111 1.80e-13 61.6
MS.gene23009.t1 MTR_7g035245 34.043 94 62 0 1 94 3 96 4.42e-13 60.5
MS.gene23009.t1 MTR_3g077560 32.979 94 63 0 1 94 21 114 1.74e-12 59.3
MS.gene23009.t1 MTR_1g015890 34.737 95 52 2 3 91 48 138 2.95e-12 59.3
MS.gene23009.t1 MTR_2g038560 31.707 82 56 0 13 94 40 121 3.97e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23009.t1 AT2G47880 75.758 99 24 0 1 99 1 99 1.14e-54 165
MS.gene23009.t1 AT2G30540 71.000 100 29 0 1 100 1 100 4.86e-54 164
MS.gene23009.t1 AT1G06830 74.227 97 25 0 1 97 1 97 2.59e-53 162
MS.gene23009.t1 AT3G62960 71.569 102 29 0 1 102 1 102 4.92e-53 161
MS.gene23009.t1 AT3G62950 53.000 100 46 1 1 99 1 100 1.01e-37 122
MS.gene23009.t1 AT2G47870 53.125 96 44 1 1 95 1 96 4.10e-36 118
MS.gene23009.t1 AT3G21460 54.737 95 43 0 1 95 1 95 1.93e-35 117
MS.gene23009.t1 AT4G15680 49.474 95 48 0 1 95 1 95 1.27e-32 109
MS.gene23009.t1 AT5G18600 50.526 95 47 0 1 95 1 95 2.30e-32 109
MS.gene23009.t1 AT4G15670 48.421 95 49 0 1 95 1 95 5.52e-32 108
MS.gene23009.t1 AT4G15660 47.423 97 51 0 1 97 1 97 9.85e-32 107
MS.gene23009.t1 AT4G15700 48.421 95 49 0 1 95 1 95 1.43e-31 107
MS.gene23009.t1 AT4G15690 48.421 95 49 0 1 95 1 95 2.67e-31 106
MS.gene23009.t1 AT5G14070 43.689 103 50 2 1 95 31 133 3.50e-26 94.7
MS.gene23009.t1 AT3G62930 42.105 95 55 0 1 95 1 95 2.48e-25 91.3
MS.gene23009.t1 AT3G02000 43.434 99 50 1 3 95 31 129 1.23e-23 88.2
MS.gene23009.t1 AT1G03020 42.105 95 55 0 1 95 1 95 1.83e-23 86.7
MS.gene23009.t1 AT1G28480 33.673 98 61 2 2 95 33 130 3.93e-15 66.2
MS.gene23009.t1 AT4G33040 39.785 93 50 2 3 90 41 132 5.21e-15 66.2
MS.gene23009.t1 AT4G28730 35.556 90 57 1 2 90 71 160 1.08e-14 66.2
MS.gene23009.t1 AT5G40370 31.915 94 64 0 1 94 3 96 1.28e-12 59.3
MS.gene23009.t1 AT5G20500 33.333 93 62 0 2 94 33 125 4.71e-12 58.5
MS.gene23009.t1 AT4G28730 38.571 70 42 1 2 70 71 140 1.12e-11 57.8
MS.gene23009.t1 AT2G20270 31.915 94 63 1 2 94 76 169 1.62e-11 57.8

Find 34 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCCATTTCCCTTCCTTC+AGG 0.237314 7.2:-6758220 None:intergenic
CCTGTTCCTGCTGTGTTCAT+TGG 0.272350 7.2:+6758271 MS.gene23009:CDS
TGTTTCAAGAACTTGGGATT+AGG 0.357723 7.2:+6758170 MS.gene23009:CDS
TCTCCATTTCCCTTCCTTCA+GGG 0.366539 7.2:-6758219 None:intergenic
AACATTCTGTTTCAAGAACT+TGG 0.368312 7.2:+6758163 MS.gene23009:CDS
GTGAAGGGACATAATTTCAT+TGG 0.393227 7.2:-6758314 None:intergenic
AGACCCTGAAGGAAGGGAAA+TGG 0.401791 7.2:+6758216 MS.gene23009:CDS
TTGAGGTTGGCATCTGAAAA+AGG 0.425553 7.2:+6758094 MS.gene23009:CDS
TGATTCAATTAGAACGAGAT+TGG 0.435807 7.2:-6758372 None:intergenic
AGAACTTGGGATTAGGCCTA+TGG 0.436071 7.2:+6758177 MS.gene23009:CDS
GTTCCTGCTGTGTTCATTGG+AGG 0.437740 7.2:+6758274 MS.gene23009:CDS
GAGAAAGCTTTGTTGAGGTT+AGG 0.441107 7.2:+6758238 MS.gene23009:CDS
TTCATTGGAGGGCAACTGAT+GGG 0.446550 7.2:+6758286 MS.gene23009:CDS
CCAATGAACACAGCAGGAAC+AGG 0.496655 7.2:-6758271 None:intergenic
GTTCATTGGAGGGCAACTGA+TGG 0.499451 7.2:+6758285 MS.gene23009:CDS
ATTGAGTGAGGGAGCCACTT+AGG 0.507881 7.2:-6758336 None:intergenic
CAATTAGAACGAGATTGGTA+TGG 0.511391 7.2:-6758367 None:intergenic
TGATCAATTTCATGAACCAT+AGG 0.516130 7.2:-6758193 None:intergenic
TTCCTGCTGTGTTCATTGGA+GGG 0.520038 7.2:+6758275 MS.gene23009:CDS
ACATTCTGTTTCAAGAACTT+GGG 0.523434 7.2:+6758164 MS.gene23009:CDS
TGCCCTCCAATGAACACAGC+AGG 0.533916 7.2:-6758277 None:intergenic
TTGATCAAGACCCTGAAGGA+AGG 0.541164 7.2:+6758209 MS.gene23009:CDS
AAGGGACATAATTTCATTGG+TGG 0.541849 7.2:-6758311 None:intergenic
ATTATGTCCCTTCACCTAAG+TGG 0.557113 7.2:+6758322 MS.gene23009:CDS
TGATCAAGACCCTGAAGGAA+GGG 0.574619 7.2:+6758210 MS.gene23009:CDS
TCATTGGAGGGCAACTGATG+GGG 0.576911 7.2:+6758287 MS.gene23009:CDS
TGAGGGAGCCACTTAGGTGA+AGG 0.580811 7.2:-6758330 None:intergenic
AAATGGAGAAAGCTTTGTTG+AGG 0.591044 7.2:+6758233 MS.gene23009:CDS
GATGGACAAAGTGTTGAGGT+TGG 0.596621 7.2:+6758081 None:intergenic
GAGGGAGCCACTTAGGTGAA+GGG 0.625802 7.2:-6758329 None:intergenic
TCAAGATGGACAAAGTGTTG+AGG 0.649690 7.2:+6758077 None:intergenic
TGGCTTGATCAATTGAGTGA+GGG 0.652206 7.2:-6758347 None:intergenic
GAAATTGATCAAGACCCTGA+AGG 0.656540 7.2:+6758205 MS.gene23009:CDS
ATGGCTTGATCAATTGAGTG+AGG 0.695900 7.2:-6758348 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACATTCTGTTTCAAGAACT+TGG + chr7.2:6758163-6758182 MS.gene23009:CDS 30.0%
ACATTCTGTTTCAAGAACTT+GGG + chr7.2:6758164-6758183 MS.gene23009:CDS 30.0%
TGATCAATTTCATGAACCAT+AGG - chr7.2:6758196-6758215 None:intergenic 30.0%
AAGGGACATAATTTCATTGG+TGG - chr7.2:6758314-6758333 None:intergenic 35.0%
CAATTAGAACGAGATTGGTA+TGG - chr7.2:6758370-6758389 None:intergenic 35.0%
GTGAAGGGACATAATTTCAT+TGG - chr7.2:6758317-6758336 None:intergenic 35.0%
TGTTTCAAGAACTTGGGATT+AGG + chr7.2:6758170-6758189 MS.gene23009:CDS 35.0%
!! AAATGGAGAAAGCTTTGTTG+AGG + chr7.2:6758233-6758252 MS.gene23009:CDS 35.0%
ATGGCTTGATCAATTGAGTG+AGG - chr7.2:6758351-6758370 None:intergenic 40.0%
ATTATGTCCCTTCACCTAAG+TGG + chr7.2:6758322-6758341 MS.gene23009:CDS 40.0%
GAAATTGATCAAGACCCTGA+AGG + chr7.2:6758205-6758224 MS.gene23009:CDS 40.0%
TGGCTTGATCAATTGAGTGA+GGG - chr7.2:6758350-6758369 None:intergenic 40.0%
TTGAGGTTGGCATCTGAAAA+AGG + chr7.2:6758094-6758113 MS.gene23009:CDS 40.0%
!! GAGAAAGCTTTGTTGAGGTT+AGG + chr7.2:6758238-6758257 MS.gene23009:CDS 40.0%
AGAACTTGGGATTAGGCCTA+TGG + chr7.2:6758177-6758196 MS.gene23009:CDS 45.0%
GTTGGCATCTGAAAAAGGTG+TGG + chr7.2:6758099-6758118 MS.gene23009:CDS 45.0%
TCTCCATTTCCCTTCCTTCA+GGG - chr7.2:6758222-6758241 None:intergenic 45.0%
TGATCAAGACCCTGAAGGAA+GGG + chr7.2:6758210-6758229 MS.gene23009:CDS 45.0%
TTCATTGGAGGGCAACTGAT+GGG + chr7.2:6758286-6758305 MS.gene23009:CDS 45.0%
TTCCTGCTGTGTTCATTGGA+GGG + chr7.2:6758275-6758294 MS.gene23009:CDS 45.0%
TTCTCCATTTCCCTTCCTTC+AGG - chr7.2:6758223-6758242 None:intergenic 45.0%
TTGATCAAGACCCTGAAGGA+AGG + chr7.2:6758209-6758228 MS.gene23009:CDS 45.0%
AGACCCTGAAGGAAGGGAAA+TGG + chr7.2:6758216-6758235 MS.gene23009:CDS 50.0%
ATTGAGTGAGGGAGCCACTT+AGG - chr7.2:6758339-6758358 None:intergenic 50.0%
CCAATGAACACAGCAGGAAC+AGG - chr7.2:6758274-6758293 None:intergenic 50.0%
CCTGTTCCTGCTGTGTTCAT+TGG + chr7.2:6758271-6758290 MS.gene23009:CDS 50.0%
GTTCATTGGAGGGCAACTGA+TGG + chr7.2:6758285-6758304 MS.gene23009:CDS 50.0%
GTTCCTGCTGTGTTCATTGG+AGG + chr7.2:6758274-6758293 MS.gene23009:CDS 50.0%
TCATTGGAGGGCAACTGATG+GGG + chr7.2:6758287-6758306 MS.gene23009:CDS 50.0%
GAGGGAGCCACTTAGGTGAA+GGG - chr7.2:6758332-6758351 None:intergenic 55.0%
TGAGGGAGCCACTTAGGTGA+AGG - chr7.2:6758333-6758352 None:intergenic 55.0%
TGCCCTCCAATGAACACAGC+AGG - chr7.2:6758280-6758299 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 6758082 6758390 6758082 ID=MS.gene23009
chr7.2 mRNA 6758082 6758390 6758082 ID=MS.gene23009.t1;Parent=MS.gene23009
chr7.2 exon 6758082 6758390 6758082 ID=MS.gene23009.t1.exon1;Parent=MS.gene23009.t1
chr7.2 CDS 6758082 6758390 6758082 ID=cds.MS.gene23009.t1;Parent=MS.gene23009.t1
Gene Sequence

>MS.gene23009

ATGGACAAAGTGTTGAGGTTGGCATCTGAAAAAGGTGTGGTGATTTTCACAAAGAGTTCATGTTGTCTATGCTATGCAGTTAACATTCTGTTTCAAGAACTTGGGATTAGGCCTATGGTTCATGAAATTGATCAAGACCCTGAAGGAAGGGAAATGGAGAAAGCTTTGTTGAGGTTAGGTTGTACTGCACCTGTTCCTGCTGTGTTCATTGGAGGGCAACTGATGGGGTCCACCAATGAAATTATGTCCCTTCACCTAAGTGGCTCCCTCACTCAATTGATCAAGCCATACCAATCTCGTTCTAATTGA

Protein sequence

>MS.gene23009.t1

MDKVLRLASEKGVVIFTKSSCCLCYAVNILFQELGIRPMVHEIDQDPEGREMEKALLRLGCTAPVPAVFIGGQLMGSTNEIMSLHLSGSLTQLIKPYQSRSN