AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058226

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058226.t1 MTR_2g034260 87.708 602 51 6 1 595 1 586 0.0 1052
MS.gene058226.t1 MTR_2g034250 84.448 598 79 4 1 595 1 587 0.0 1014
MS.gene058226.t1 MTR_2g034280 76.949 603 105 7 1 595 1 577 0.0 912
MS.gene058226.t1 MTR_7g109580 44.715 492 249 7 104 595 88 556 2.98e-135 406
MS.gene058226.t1 MTR_1g086970 43.515 478 242 8 124 595 25 480 9.04e-126 379
MS.gene058226.t1 MTR_3g065980 25.777 547 324 11 81 594 44 541 6.36e-54 192
MS.gene058226.t1 MTR_7g027190 29.975 407 249 11 204 593 286 673 5.21e-47 176
MS.gene058226.t1 MTR_8g442410 29.592 392 253 9 211 593 148 525 2.30e-43 163
MS.gene058226.t1 MTR_4g104020 28.499 393 257 10 211 593 142 520 1.66e-41 157
MS.gene058226.t1 MTR_4g064150 28.499 393 262 7 208 592 351 732 2.19e-41 160
MS.gene058226.t1 MTR_4g064200 29.124 388 250 10 213 592 279 649 9.28e-41 157
MS.gene058226.t1 MTR_7g074650 31.635 373 232 11 222 593 442 792 4.31e-39 153
MS.gene058226.t1 MTR_4g076140 27.347 490 260 13 179 593 1 469 2.29e-38 147
MS.gene058226.t1 MTR_2g097463 27.456 397 244 8 213 592 285 654 2.60e-37 147
MS.gene058226.t1 MTR_7g062120 28.571 392 259 9 209 593 363 740 3.13e-37 147
MS.gene058226.t1 MTR_2g097473 28.792 389 249 8 213 592 284 653 4.06e-37 147
MS.gene058226.t1 MTR_4g102790 27.532 385 260 9 215 592 289 661 7.85e-36 143
MS.gene058226.t1 MTR_2g097467 27.649 387 256 7 213 592 284 653 9.23e-36 142
MS.gene058226.t1 MTR_4g064160 26.355 406 240 9 213 592 311 683 1.21e-35 142
MS.gene058226.t1 MTR_1g069725 27.027 407 267 10 188 592 84 462 5.48e-35 138
MS.gene058226.t1 MTR_5g094450 25.773 388 266 7 215 593 158 532 7.42e-35 139
MS.gene058226.t1 MTR_4g064120 25.648 386 266 7 207 583 243 616 1.20e-34 139
MS.gene058226.t1 MTR_2g097410 26.065 399 260 8 209 592 362 740 3.41e-34 138
MS.gene058226.t1 MTR_2g026250 25.714 385 267 8 215 593 225 596 1.59e-33 135
MS.gene058226.t1 MTR_4g077760 25.781 384 262 8 215 593 190 555 1.75e-33 135
MS.gene058226.t1 MTR_4g064180 25.648 386 266 7 207 583 243 616 2.10e-33 135
MS.gene058226.t1 MTR_4g133660 24.740 384 270 8 215 592 172 542 4.34e-33 134
MS.gene058226.t1 MTR_4g076020 28.647 377 229 10 222 593 88 429 5.80e-33 132
MS.gene058226.t1 MTR_3g022830 27.739 429 240 10 219 594 26 437 3.29e-32 129
MS.gene058226.t1 MTR_2g097350 24.227 388 275 6 210 592 266 639 1.83e-31 130
MS.gene058226.t1 MTR_2g097390 23.711 388 277 7 210 592 267 640 1.31e-30 127
MS.gene058226.t1 MTR_5g097480 25.740 338 229 7 219 548 178 501 1.85e-30 126
MS.gene058226.t1 MTR_3g089055 27.481 393 241 12 219 593 199 565 2.51e-30 125
MS.gene058226.t1 MTR_3g089055 27.481 393 241 12 219 593 204 570 2.56e-30 125
MS.gene058226.t1 MTR_3g072710 27.206 408 256 14 208 592 110 499 3.95e-30 124
MS.gene058226.t1 MTR_2g097310 23.454 388 278 7 210 592 264 637 7.93e-30 125
MS.gene058226.t1 MTR_1g029420 25.954 393 247 11 219 593 226 592 3.39e-29 122
MS.gene058226.t1 MTR_3g056110 24.870 386 268 8 215 593 172 542 3.60e-29 122
MS.gene058226.t1 MTR_2g082090 23.755 522 342 14 109 592 75 578 3.31e-28 119
MS.gene058226.t1 MTR_1g096030 25.238 420 264 13 203 595 65 461 5.24e-27 114
MS.gene058226.t1 MTR_5g058860 26.150 413 266 12 203 592 100 496 5.44e-27 115
MS.gene058226.t1 MTR_2g097380 23.901 364 229 10 234 592 240 560 1.35e-25 111
MS.gene058226.t1 MTR_4g097080 23.559 399 287 6 198 593 120 503 8.85e-25 108
MS.gene058226.t1 MTR_8g093070 27.250 400 249 15 205 592 126 495 3.73e-24 106
MS.gene058226.t1 MTR_7g057230 26.598 391 245 12 217 592 293 656 5.53e-21 97.8
MS.gene058226.t1 MTR_5g009080 32.237 152 102 1 442 593 328 478 6.74e-21 96.7
MS.gene058226.t1 MTR_5g009080 27.016 248 165 6 194 441 33 264 2.83e-19 91.7
MS.gene058226.t1 MTR_5g015490 24.103 390 267 10 219 593 115 490 6.84e-21 96.7
MS.gene058226.t1 MTR_4g026485 25.195 385 258 11 214 593 265 624 2.69e-19 92.4
MS.gene058226.t1 MTR_6g047750 30.216 278 160 10 334 593 401 662 3.14e-19 92.0
MS.gene058226.t1 MTR_2g099110 24.221 289 204 6 219 504 75 351 7.40e-19 90.1
MS.gene058226.t1 MTR_0092s0100 23.057 386 266 10 214 593 371 731 3.20e-17 85.9
MS.gene058226.t1 MTR_0092s0100 23.057 386 266 10 214 593 368 728 3.27e-17 85.9
MS.gene058226.t1 MTR_2g089100 20.299 335 231 7 219 526 53 378 6.82e-14 74.7
MS.gene058226.t1 MTR_8g077940 25.634 355 230 14 216 560 180 510 2.71e-13 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058226.t1 AT3G03450 32.061 393 248 7 205 594 170 546 4.30e-61 212
MS.gene058226.t1 AT5G17490 30.334 389 246 8 209 594 151 517 4.97e-54 192
MS.gene058226.t1 AT2G01570 29.695 394 251 7 205 594 211 582 2.73e-53 192
MS.gene058226.t1 AT1G14920 29.771 393 252 7 205 594 159 530 2.92e-52 187
MS.gene058226.t1 AT1G66350 29.008 393 245 6 207 594 144 507 1.68e-51 185
MS.gene058226.t1 AT5G41920 31.218 394 243 11 205 593 30 400 3.19e-46 168
MS.gene058226.t1 AT5G59450 30.848 389 249 9 213 593 222 598 2.04e-44 167
MS.gene058226.t1 AT3G46600 29.921 381 247 8 219 593 5 371 5.07e-43 158
MS.gene058226.t1 AT3G46600 29.563 389 254 8 211 593 205 579 1.19e-42 162
MS.gene058226.t1 AT3G46600 29.563 389 254 8 211 593 173 547 4.44e-42 160
MS.gene058226.t1 AT1G07530 28.680 394 261 6 206 592 384 764 1.54e-40 157
MS.gene058226.t1 AT3G54220 30.914 372 237 10 222 592 297 649 8.13e-40 154
MS.gene058226.t1 AT1G55580 27.851 456 277 8 177 593 3 445 2.44e-39 150
MS.gene058226.t1 AT2G29065 25.065 387 265 7 219 592 253 627 6.68e-38 149
MS.gene058226.t1 AT2G29060 27.877 391 262 8 210 592 313 691 4.04e-37 147
MS.gene058226.t1 AT2G29060 28.133 391 261 7 210 592 276 654 4.24e-37 147
MS.gene058226.t1 AT1G63100 25.063 399 269 8 210 592 269 653 1.53e-35 142
MS.gene058226.t1 AT1G63100 25.063 399 269 8 210 592 244 628 1.80e-35 142
MS.gene058226.t1 AT2G37650 28.974 390 258 8 209 593 326 701 2.57e-35 142
MS.gene058226.t1 AT2G37650 28.974 390 258 8 209 593 338 713 3.11e-35 141
MS.gene058226.t1 AT4G17230 25.648 386 265 8 215 593 155 525 1.06e-34 138
MS.gene058226.t1 AT4G17230 25.648 386 265 8 215 593 155 525 1.06e-34 138
MS.gene058226.t1 AT1G07520 24.685 397 283 7 204 592 140 528 1.58e-34 137
MS.gene058226.t1 AT1G07520 24.685 397 283 7 204 592 140 528 1.58e-34 137
MS.gene058226.t1 AT5G66770 26.700 397 266 10 206 593 204 584 1.61e-34 138
MS.gene058226.t1 AT1G07520 24.685 397 283 7 204 592 304 692 3.11e-34 138
MS.gene058226.t1 AT1G07520 24.685 397 283 7 204 592 305 693 3.59e-34 138
MS.gene058226.t1 AT2G04890 26.546 388 260 9 210 593 47 413 2.29e-32 129
MS.gene058226.t1 AT3G50650 27.248 367 237 9 238 593 195 542 2.83e-31 128
MS.gene058226.t1 AT5G48150 23.377 385 276 7 215 593 119 490 9.87e-31 126
MS.gene058226.t1 AT5G48150 23.377 385 276 7 215 593 119 490 9.87e-31 126
MS.gene058226.t1 AT5G48150 23.377 385 276 7 215 593 119 490 9.87e-31 126
MS.gene058226.t1 AT5G48150 23.377 385 276 7 215 593 119 490 9.87e-31 126
MS.gene058226.t1 AT1G50600 24.211 380 270 6 219 593 160 526 4.60e-28 119
MS.gene058226.t1 AT1G50600 24.211 380 270 6 219 593 231 597 8.91e-28 118
MS.gene058226.t1 AT1G50600 24.211 380 270 6 219 593 235 601 1.32e-27 118
MS.gene058226.t1 AT4G08250 25.553 407 272 12 199 592 88 476 5.05e-24 106
MS.gene058226.t1 AT1G21450 25.439 342 229 9 219 550 226 551 2.96e-22 101
MS.gene058226.t1 AT1G50420 29.368 269 167 7 182 442 15 268 7.42e-20 93.2
MS.gene058226.t1 AT1G50420 31.410 156 96 3 443 593 330 479 1.06e-18 89.7
MS.gene058226.t1 AT2G45160 24.000 400 255 12 215 593 268 639 2.53e-19 92.4
MS.gene058226.t1 AT4G00150 26.500 400 227 18 213 593 203 554 1.99e-18 89.4
MS.gene058226.t1 AT4G37650 21.883 393 279 9 219 593 147 529 2.25e-18 89.0
MS.gene058226.t1 AT3G60630 25.083 303 209 8 300 593 329 622 2.12e-16 83.2
MS.gene058226.t1 AT3G49950 24.747 396 257 13 219 592 31 407 2.45e-16 82.0
MS.gene058226.t1 AT4G36710 23.716 409 279 13 195 594 102 486 3.03e-16 82.0
MS.gene058226.t1 AT3G13840 25.850 294 189 10 207 488 137 413 7.05e-13 71.6

Find 77 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATCCAGTTAATGATTT+TGG 0.114608 2.4:+56480248 MS.gene058226:CDS
AACCATTACTATAGGGTTTA+TGG 0.150240 2.4:-56481158 None:intergenic
ATGGAAAACTTGTGCAATTT+TGG 0.214814 2.4:+56479807 MS.gene058226:CDS
TAATGAACCACGCGCTTAAC+TGG 0.226146 2.4:-56480557 None:intergenic
TTTCATTTCACTAAGTTTCT+TGG 0.235784 2.4:-56479892 None:intergenic
CTTCAAAGTGTCTATGTTTC+CGG 0.238242 2.4:-56480915 None:intergenic
GAGTCTCTTTGCTCTAATTT+TGG 0.258364 2.4:+56479963 MS.gene058226:CDS
CTTGATATGATCCTTGTTCT+TGG 0.265549 2.4:-56480037 None:intergenic
TTGATTCTTAATCACCCTTT+TGG 0.265653 2.4:+56480380 MS.gene058226:CDS
CAAACAGAAACAGAGTTAAT+TGG 0.268686 2.4:-56481559 None:intergenic
GTTCTTGGTGATGATACTTT+AGG 0.270808 2.4:-56480022 None:intergenic
TTGTCCATTGTAACCCTTTA+CGG 0.295916 2.4:-56480823 None:intergenic
CTACTGGTGCTAAAGGAATT+TGG 0.313681 2.4:-56480166 None:intergenic
CAATTAACTCTGTTTCTGTT+TGG 0.337936 2.4:+56481560 MS.gene058226:CDS
TCGATTGAAGTGCTTGCATT+AGG 0.355406 2.4:-56480847 None:intergenic
AAGGAATTTGGTAGAATTGT+TGG 0.356422 2.4:-56480154 None:intergenic
CCGTGACAACCATTACTATA+GGG 0.362305 2.4:-56481165 None:intergenic
ATCAAACCAAAATCATTAAC+TGG 0.365703 2.4:-56480254 None:intergenic
ACCTCTTGAGGATCAAATAA+AGG 0.383647 2.4:-56480662 None:intergenic
AATGGTCATTGTCTTCTTGT+TGG 0.397166 2.4:+56481523 MS.gene058226:CDS
GACAAACAATATGCAAAAGA+TGG 0.402851 2.4:+56480637 MS.gene058226:CDS
TCTTGCACATTTGATATGAA+TGG 0.412387 2.4:+56481505 MS.gene058226:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.414068 2.4:+56480599 MS.gene058226:CDS
GTCTTTGTATCAAGCTGAAT+TGG 0.419059 2.4:+56481456 MS.gene058226:CDS
GTAGATATATTGAAGAACTA+TGG 0.423065 2.4:+56480185 MS.gene058226:CDS
GAAGATCTAGGAGACTATAA+TGG 0.427527 2.4:+56479936 MS.gene058226:CDS
GATTCTTTGTTGAAGAGCTT+CGG 0.433882 2.4:-56480585 None:intergenic
TCTTCGTCGCAATCTGCATC+AGG 0.438613 2.4:+56480356 MS.gene058226:CDS
CTCTCATGATTGTTTCAAGT+TGG 0.456214 2.4:-56481126 None:intergenic
AGGAAATCATCTTCTTCTGA+TGG 0.462485 2.4:-56480002 None:intergenic
AGGAATATTGTGGCTGAAGA+AGG 0.463268 2.4:+56481343 MS.gene058226:CDS
CAAAATCATTAACTGGATGA+AGG 0.470878 2.4:-56480247 None:intergenic
GAAGATATCATGAAGATAGC+TGG 0.473521 2.4:+56480311 MS.gene058226:CDS
TCCGTGACAACCATTACTAT+AGG 0.473792 2.4:-56481166 None:intergenic
TGTCTTCTTGTTGGATGGAA+GGG 0.477569 2.4:+56481532 MS.gene058226:CDS
ATTCGATACAATTATTGTAT+CGG 0.477780 2.4:+56481003 MS.gene058226:CDS
AATATATCTACTGGTGCTAA+AGG 0.478512 2.4:-56480173 None:intergenic
AGATCCTTGGTTACCTCTTG+AGG 0.485987 2.4:-56480674 None:intergenic
TAGTTCTTCAATATATCTAC+TGG 0.487136 2.4:-56480182 None:intergenic
TTGATCTCGAAATCCGTAAA+GGG 0.491083 2.4:+56480810 MS.gene058226:CDS
TTGTCTTCTTGTTGGATGGA+AGG 0.499303 2.4:+56481531 MS.gene058226:CDS
AAGCGCTGGATTTAGATCCT+TGG 0.500832 2.4:-56480687 None:intergenic
CCCTATAGTAATGGTTGTCA+CGG 0.506352 2.4:+56481165 MS.gene058226:CDS
GTCATTGTCTTCTTGTTGGA+TGG 0.509549 2.4:+56481527 MS.gene058226:CDS
GAGTCTTTATCTTCGAAGAC+AGG 0.510353 2.4:+56480530 MS.gene058226:CDS
ATAATTGTATCGAATACAAA+AGG 0.522577 2.4:-56480995 None:intergenic
CCTTGAGCTTCTAAAGATAA+CGG 0.523094 2.4:+56480883 MS.gene058226:CDS
TGACACTGGTAAAAGATTGA+AGG 0.529885 2.4:+56480949 MS.gene058226:CDS
AAAATCATTAACTGGATGAA+GGG 0.532257 2.4:-56480246 None:intergenic
ATTGATCTCGAAATCCGTAA+AGG 0.532305 2.4:+56480809 MS.gene058226:CDS
CCGTTATCTTTAGAAGCTCA+AGG 0.533344 2.4:-56480883 None:intergenic
AAGATAACGGCTATTGTGAC+CGG 0.536013 2.4:+56480896 MS.gene058226:CDS
CGTTATCTTTAGAAGCTCAA+GGG 0.536414 2.4:-56480882 None:intergenic
TTTATCTGATGAAGAGAAAG+AGG 0.536955 2.4:+56480418 MS.gene058226:CDS
CTTGAGTTTCAATATAGTTG+AGG 0.537962 2.4:+56479833 MS.gene058226:CDS
AACCATAAACCCTATAGTAA+TGG 0.546098 2.4:+56481156 MS.gene058226:CDS
ACAAACAATATGCAAAAGAT+GGG 0.551770 2.4:+56480638 MS.gene058226:CDS
AAGAGGTTACTACCTGATGA+AGG 0.555954 2.4:+56480218 MS.gene058226:CDS
GACATGAATCTTCTTAGCAT+CGG 0.571430 2.4:-56480786 None:intergenic
ACCTTTATTTGATCCTCAAG+AGG 0.572203 2.4:+56480661 MS.gene058226:CDS
AATGAACCACGCGCTTAACT+GGG 0.580982 2.4:-56480556 None:intergenic
TCATGGTATTAGGAATATTG+TGG 0.583709 2.4:+56481333 MS.gene058226:CDS
AATGAACCATGTCAAAGTGT+AGG 0.587676 2.4:-56480079 None:intergenic
AAGTATCATCACCAAGAACA+AGG 0.600969 2.4:+56480026 MS.gene058226:CDS
GTTGCTAAAAGATTTCCTTG+TGG 0.611038 2.4:+56481478 MS.gene058226:CDS
TTAGCTTGTGCTGAGAAAGT+CGG 0.613798 2.4:+56480464 MS.gene058226:CDS
TGATAACCTACACTTTGACA+TGG 0.617264 2.4:+56480073 MS.gene058226:CDS
AGACTCTGAGGAAACTGTTG+TGG 0.618556 2.4:+56481060 MS.gene058226:CDS
ACTATGGCAAATGCTTCAAG+AGG 0.626795 2.4:+56480201 MS.gene058226:CDS
AAATCCGTAAAGGGTTACAA+TGG 0.629230 2.4:+56480819 MS.gene058226:CDS
AAGCATATAGTTGATGACAC+TGG 0.630401 2.4:+56480935 MS.gene058226:CDS
GTTATCTTTAGAAGCTCAAG+GGG 0.631960 2.4:-56480881 None:intergenic
TCATGAAGATAGCTGGAACA+AGG 0.635820 2.4:+56480318 MS.gene058226:CDS
GAAATGTGAAGATTGATGTG+TGG 0.658264 2.4:+56481383 MS.gene058226:CDS
TGATCCTCAAGAGGTAACCA+AGG 0.673485 2.4:+56480670 MS.gene058226:CDS
AGGTAACCCAGTTAAGCGCG+TGG 0.690106 2.4:+56480550 MS.gene058226:CDS
AATGTGCAAGAATTTCCACA+AGG 0.746520 2.4:-56481493 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTGAAATTTTACAAAA+TGG - chr2.4:56480734-56480753 None:intergenic 15.0%
!! ATAATTGTATCGAATACAAA+AGG - chr2.4:56480998-56481017 None:intergenic 20.0%
!! ATTCGATACAATTATTGTAT+CGG + chr2.4:56481003-56481022 MS.gene058226:CDS 20.0%
!!! TTCATCCAATGATATTTTTT+GGG + chr2.4:56479866-56479885 MS.gene058226:CDS 20.0%
! AAAATCATTAACTGGATGAA+GGG - chr2.4:56480249-56480268 None:intergenic 25.0%
! ACAAACAATATGCAAAAGAT+GGG + chr2.4:56480638-56480657 MS.gene058226:CDS 25.0%
! ATCAAACCAAAATCATTAAC+TGG - chr2.4:56480257-56480276 None:intergenic 25.0%
! GTAGATATATTGAAGAACTA+TGG + chr2.4:56480185-56480204 MS.gene058226:CDS 25.0%
! TAGTTCTTCAATATATCTAC+TGG - chr2.4:56480185-56480204 None:intergenic 25.0%
! TTTCATTTCACTAAGTTTCT+TGG - chr2.4:56479895-56479914 None:intergenic 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.4:56481411-56481430 MS.gene058226:CDS 25.0%
!!! GTTCATCCAATGATATTTTT+TGG + chr2.4:56479865-56479884 MS.gene058226:CDS 25.0%
!!! TGTATTTTAGTCATGGTATT+AGG + chr2.4:56481323-56481342 MS.gene058226:CDS 25.0%
AACCATAAACCCTATAGTAA+TGG + chr2.4:56481156-56481175 MS.gene058226:CDS 30.0%
AACCATTACTATAGGGTTTA+TGG - chr2.4:56481161-56481180 None:intergenic 30.0%
AAGGAATTTGGTAGAATTGT+TGG - chr2.4:56480157-56480176 None:intergenic 30.0%
CAAAATCATTAACTGGATGA+AGG - chr2.4:56480250-56480269 None:intergenic 30.0%
CAAACAGAAACAGAGTTAAT+TGG - chr2.4:56481562-56481581 None:intergenic 30.0%
CAGAGAAACAAAAACCAAAA+GGG - chr2.4:56480397-56480416 None:intergenic 30.0%
CTTGAGTTTCAATATAGTTG+AGG + chr2.4:56479833-56479852 MS.gene058226:CDS 30.0%
GACAAACAATATGCAAAAGA+TGG + chr2.4:56480637-56480656 MS.gene058226:CDS 30.0%
GGTTTCCCAAAAAATATCAT+TGG - chr2.4:56479874-56479893 None:intergenic 30.0%
TCATGGTATTAGGAATATTG+TGG + chr2.4:56481333-56481352 MS.gene058226:CDS 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.4:56481505-56481524 MS.gene058226:CDS 30.0%
TTTATCTGATGAAGAGAAAG+AGG + chr2.4:56480418-56480437 MS.gene058226:CDS 30.0%
! AATATATCTACTGGTGCTAA+AGG - chr2.4:56480176-56480195 None:intergenic 30.0%
! ATGGAAAACTTGTGCAATTT+TGG + chr2.4:56479807-56479826 MS.gene058226:CDS 30.0%
! CTTCATCCAGTTAATGATTT+TGG + chr2.4:56480248-56480267 MS.gene058226:CDS 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.4:56480380-56480399 MS.gene058226:CDS 30.0%
! TTTTCTGTAGAAGAAGATCT+AGG + chr2.4:56479924-56479943 MS.gene058226:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.4:56481228-56481247 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.4:56481406-56481425 MS.gene058226:CDS 30.0%
!! CAATTAACTCTGTTTCTGTT+TGG + chr2.4:56481560-56481579 MS.gene058226:CDS 30.0%
!! TGCATTTTTCGATAGTTTTG+AGG + chr2.4:56481255-56481274 MS.gene058226:CDS 30.0%
!!! AGTTTTGAGGATTGTATGAA+AGG + chr2.4:56481268-56481287 MS.gene058226:CDS 30.0%
!!! GAGTCAATGTATTTTAGTCA+TGG + chr2.4:56481316-56481335 MS.gene058226:CDS 30.0%
AAATCCGTAAAGGGTTACAA+TGG + chr2.4:56480819-56480838 MS.gene058226:CDS 35.0%
AAGTATCATCACCAAGAACA+AGG + chr2.4:56480026-56480045 MS.gene058226:CDS 35.0%
AATGAACCATGTCAAAGTGT+AGG - chr2.4:56480082-56480101 None:intergenic 35.0%
AATGGTCATTGTCTTCTTGT+TGG + chr2.4:56481523-56481542 MS.gene058226:CDS 35.0%
AATGTGCAAGAATTTCCACA+AGG - chr2.4:56481496-56481515 None:intergenic 35.0%
ACCTCTTGAGGATCAAATAA+AGG - chr2.4:56480665-56480684 None:intergenic 35.0%
AGGAAATCATCTTCTTCTGA+TGG - chr2.4:56480005-56480024 None:intergenic 35.0%
ATTGATCTCGAAATCCGTAA+AGG + chr2.4:56480809-56480828 MS.gene058226:CDS 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.4:56480599-56480618 MS.gene058226:CDS 35.0%
CCAGAGAAACAAAAACCAAA+AGG - chr2.4:56480398-56480417 None:intergenic 35.0%
CTTGATATGATCCTTGTTCT+TGG - chr2.4:56480040-56480059 None:intergenic 35.0%
GAAATGTGAAGATTGATGTG+TGG + chr2.4:56481383-56481402 MS.gene058226:CDS 35.0%
GAAGATATCATGAAGATAGC+TGG + chr2.4:56480311-56480330 MS.gene058226:CDS 35.0%
GAAGATCTAGGAGACTATAA+TGG + chr2.4:56479936-56479955 MS.gene058226:CDS 35.0%
GACATGAATCTTCTTAGCAT+CGG - chr2.4:56480789-56480808 None:intergenic 35.0%
GATTCTTTGTTGAAGAGCTT+CGG - chr2.4:56480588-56480607 None:intergenic 35.0%
GTCTTTGTATCAAGCTGAAT+TGG + chr2.4:56481456-56481475 MS.gene058226:CDS 35.0%
GTTGCTAAAAGATTTCCTTG+TGG + chr2.4:56481478-56481497 MS.gene058226:CDS 35.0%
TCCACTATGTGACACAAAAA+AGG + chr2.4:56480130-56480149 MS.gene058226:CDS 35.0%
TGACACTGGTAAAAGATTGA+AGG + chr2.4:56480949-56480968 MS.gene058226:CDS 35.0%
TGATAACCTACACTTTGACA+TGG + chr2.4:56480073-56480092 MS.gene058226:CDS 35.0%
TTGATCTCGAAATCCGTAAA+GGG + chr2.4:56480810-56480829 MS.gene058226:CDS 35.0%
TTGTCCATTGTAACCCTTTA+CGG - chr2.4:56480826-56480845 None:intergenic 35.0%
! ACCTTTATTTGATCCTCAAG+AGG + chr2.4:56480661-56480680 MS.gene058226:CDS 35.0%
! CCTTGAGCTTCTAAAGATAA+CGG + chr2.4:56480883-56480902 MS.gene058226:CDS 35.0%
! CGTTATCTTTAGAAGCTCAA+GGG - chr2.4:56480885-56480904 None:intergenic 35.0%
! CTCTCATGATTGTTTCAAGT+TGG - chr2.4:56481129-56481148 None:intergenic 35.0%
! CTTCAAAGTGTCTATGTTTC+CGG - chr2.4:56480918-56480937 None:intergenic 35.0%
! GAGTCTCTTTGCTCTAATTT+TGG + chr2.4:56479963-56479982 MS.gene058226:CDS 35.0%
! GTTATCTTTAGAAGCTCAAG+GGG - chr2.4:56480884-56480903 None:intergenic 35.0%
! TCTTTTCATGATAGACTCTG+AGG + chr2.4:56481048-56481067 MS.gene058226:CDS 35.0%
!! AAGCATATAGTTGATGACAC+TGG + chr2.4:56480935-56480954 MS.gene058226:CDS 35.0%
!! GTTCTTGGTGATGATACTTT+AGG - chr2.4:56480025-56480044 None:intergenic 35.0%
!! TTTTTGTGTCACATAGTGGA+GGG - chr2.4:56480130-56480149 None:intergenic 35.0%
!!! CCTTTTGGTTTTTGTTTCTC+TGG + chr2.4:56480395-56480414 MS.gene058226:CDS 35.0%
!!! TCCTTTTTTGTGTCACATAG+TGG - chr2.4:56480134-56480153 None:intergenic 35.0%
!!! TTTTTTGTGTCACATAGTGG+AGG - chr2.4:56480131-56480150 None:intergenic 35.0%
AAGAGGTTACTACCTGATGA+AGG + chr2.4:56480218-56480237 MS.gene058226:CDS 40.0%
ACTATGGCAAATGCTTCAAG+AGG + chr2.4:56480201-56480220 MS.gene058226:CDS 40.0%
AGGAATATTGTGGCTGAAGA+AGG + chr2.4:56481343-56481362 MS.gene058226:CDS 40.0%
CCCTATAGTAATGGTTGTCA+CGG + chr2.4:56481165-56481184 MS.gene058226:CDS 40.0%
CCGTGACAACCATTACTATA+GGG - chr2.4:56481168-56481187 None:intergenic 40.0%
GAGTCTTTATCTTCGAAGAC+AGG + chr2.4:56480530-56480549 MS.gene058226:CDS 40.0%
GTCATTGTCTTCTTGTTGGA+TGG + chr2.4:56481527-56481546 MS.gene058226:CDS 40.0%
TCATGAAGATAGCTGGAACA+AGG + chr2.4:56480318-56480337 MS.gene058226:CDS 40.0%
TCCGTGACAACCATTACTAT+AGG - chr2.4:56481169-56481188 None:intergenic 40.0%
TGTCTTCTTGTTGGATGGAA+GGG + chr2.4:56481532-56481551 MS.gene058226:CDS 40.0%
TTGTCTTCTTGTTGGATGGA+AGG + chr2.4:56481531-56481550 MS.gene058226:CDS 40.0%
! AAGATAACGGCTATTGTGAC+CGG + chr2.4:56480896-56480915 MS.gene058226:CDS 40.0%
! CCGTTATCTTTAGAAGCTCA+AGG - chr2.4:56480886-56480905 None:intergenic 40.0%
! CTACTGGTGCTAAAGGAATT+TGG - chr2.4:56480169-56480188 None:intergenic 40.0%
! TCGATTGAAGTGCTTGCATT+AGG - chr2.4:56480850-56480869 None:intergenic 40.0%
! TGAAGGGTTTTTCCTTCATC+AGG - chr2.4:56480233-56480252 None:intergenic 40.0%
!! TTAGCTTGTGCTGAGAAAGT+CGG + chr2.4:56480464-56480483 MS.gene058226:CDS 40.0%
AATGAACCACGCGCTTAACT+GGG - chr2.4:56480559-56480578 None:intergenic 45.0%
AGATCCTTGGTTACCTCTTG+AGG - chr2.4:56480677-56480696 None:intergenic 45.0%
TAATGAACCACGCGCTTAAC+TGG - chr2.4:56480560-56480579 None:intergenic 45.0%
TGATCCTCAAGAGGTAACCA+AGG + chr2.4:56480670-56480689 MS.gene058226:CDS 45.0%
! AGACTCTGAGGAAACTGTTG+TGG + chr2.4:56481060-56481079 MS.gene058226:CDS 45.0%
!! AAGCGCTGGATTTAGATCCT+TGG - chr2.4:56480690-56480709 None:intergenic 45.0%
!! CATAGTGGAGGGTCTTTTGT+TGG - chr2.4:56480119-56480138 None:intergenic 45.0%
TCAAAAAACGCGACAAGCGC+TGG - chr2.4:56480704-56480723 None:intergenic 50.0%
TCTTCGTCGCAATCTGCATC+AGG + chr2.4:56480356-56480375 MS.gene058226:CDS 50.0%
AGGTAACCCAGTTAAGCGCG+TGG + chr2.4:56480550-56480569 MS.gene058226:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 56479807 56481594 56479807 ID=MS.gene058226
chr2.4 mRNA 56479807 56481594 56479807 ID=MS.gene058226.t1;Parent=MS.gene058226
chr2.4 exon 56479807 56481594 56479807 ID=MS.gene058226.t1.exon1;Parent=MS.gene058226.t1
chr2.4 CDS 56479807 56481594 56479807 ID=cds.MS.gene058226.t1;Parent=MS.gene058226.t1
Gene Sequence

>MS.gene058226

ATGGAAAACTTGTGCAATTTTGGTGACTTGAGTTTCAATATAGTTGAGGATAAACTTAGTTCATCCAATGATATTTTTTGGGAAACCAAGAAACTTAGTGAAATGAAAAATGTTCAATTTTCTGTAGAAGAAGATCTAGGAGACTATAATGGAATTGAGTCTCTTTGCTCTAATTTTGGTCTCTTTCAAGATGTTCCATCAGAAGAAGATGATTTCCTAAAGTATCATCACCAAGAACAAGGATCATATCAAGATTATGAAACTTTTGATAACCTACACTTTGACATGGTTCATTTTGATGAACAATGTCCAACAAAAGACCCTCCACTATGTGACACAAAAAAGGACCAACAATTCTACCAAATTCCTTTAGCACCAGTAGATATATTGAAGAACTATGGCAAATGCTTCAAGAGGTTACTACCTGATGAAGGAAAAACCCTTCATCCAGTTAATGATTTTGGTTTGATCACAAATAATGAAAATGAGAGTAAGTTGTCAACTGAAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGCAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTGTTTCTCTGGTTTATCTGATGAAGAGAAAGAGGATGTTTCACTTGTTGAATCACTCTTAGCTTGTGCTGAGAAAGTCGGATATCAACAATATCAACGCGCTAGAAAATTGTTATTACAAATCGAGTCTTTATCTTCGAAGACAGGTAACCCAGTTAAGCGCGTGGTTCATTATTTTGCCGAAGCTCTTCAACAAAGAATCGACAAAGAAACAGGAAGAGTTTCTGTTTTGACAAACAATATGCAAAAGATGGGACCTTTATTTGATCCTCAAGAGGTAACCAAGGATCTAAATCCAGCGCTTGTCGCGTTTTTTGAAGATCTTCCATTTTGTAAAATTTCAATGTTCACATGTGTACAAACTTTAATAGAAAATGTAACCGATGCTAAGAAGATTCATGTCATTGATCTCGAAATCCGTAAAGGGTTACAATGGACAATCCTAATGCAAGCACTTCAATCGAGAAACAAATGTCCCCTTGAGCTTCTAAAGATAACGGCTATTGTGACCGGAAACATAGACACTTTGAAGCATATAGTTGATGACACTGGTAAAAGATTGAAGGACTTTGCGCGATATTTGAACATACCTTTTGTATTCGATACAATTATTGTATCGGATTTATTACATCTTAGAGAAGATCTTTTCATGATAGACTCTGAGGAAACTGTTGTGGTTTACTCTCAGTTTGCGCTTCGAAGCAAGATTCAACAATCAGACCAACTTGAAACAATCATGAGAGTAGTTAGAACCATAAACCCTATAGTAATGGTTGTCACGGAGATTGAAGCTAATCACAATTCTAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCATTTTTCGATAGTTTTGAGGATTGTATGAAAGGTGATGAGAAAAACAGATTTATTCTAGAGTCAATGTATTTTAGTCATGGTATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGAAATGTGAAGATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTTGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTGGTTGCTAAAAGATTTCCTTGTGGAAATTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTGTTGGATGGAAGGGAACACCAATTAACTCTGTTTCTGTTTGGAAATTCCTTTGA
Protein sequence

>MS.gene058226.t1

MENLCNFGDLSFNIVEDKLSSSNDIFWETKKLSEMKNVQFSVEEDLGDYNGIESLCSNFGLFQDVPSEEDDFLKYHHQEQGSYQDYETFDNLHFDMVHFDEQCPTKDPPLCDTKKDQQFYQIPLAPVDILKNYGKCFKRLLPDEGKTLHPVNDFGLITNNENESKLSTEDIMKIAGTRFIQSSSSSQSASGLILNHPFGFCFSGLSDEEKEDVSLVESLLACAEKVGYQQYQRARKLLLQIESLSSKTGNPVKRVVHYFAEALQQRIDKETGRVSVLTNNMQKMGPLFDPQEVTKDLNPALVAFFEDLPFCKISMFTCVQTLIENVTDAKKIHVIDLEIRKGLQWTILMQALQSRNKCPLELLKITAIVTGNIDTLKHIVDDTGKRLKDFARYLNIPFVFDTIIVSDLLHLREDLFMIDSEETVVVYSQFALRSKIQQSDQLETIMRVVRTINPIVMVVTEIEANHNSKSFVNRFIEALFYFSAFFDSFEDCMKGDEKNRFILESMYFSHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFPCGNSCTFDMNGHCLLVGWKGTPINSVSVWKFL