AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058227

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058227.t1 MTR_2g034250 88.982 599 35 3 1 580 1 587 0.0 1064
MS.gene058227.t1 MTR_2g034260 78.512 605 86 8 1 580 1 586 0.0 927
MS.gene058227.t1 MTR_2g034280 74.587 606 99 9 1 580 1 577 0.0 866
MS.gene058227.t1 MTR_7g109580 44.937 474 240 5 107 580 104 556 2.19e-132 398
MS.gene058227.t1 MTR_1g086970 44.533 503 249 10 84 580 2 480 3.81e-131 392
MS.gene058227.t1 MTR_3g065980 28.837 430 272 7 165 579 131 541 1.16e-56 199
MS.gene058227.t1 MTR_7g027190 30.100 402 253 8 190 578 287 673 6.76e-45 169
MS.gene058227.t1 MTR_4g104020 29.668 391 255 9 196 578 142 520 4.19e-44 164
MS.gene058227.t1 MTR_8g442410 30.000 400 241 10 196 578 148 525 1.39e-42 160
MS.gene058227.t1 MTR_4g064150 29.367 395 256 9 193 577 351 732 5.34e-42 161
MS.gene058227.t1 MTR_4g064200 29.639 388 248 10 198 577 279 649 1.29e-41 159
MS.gene058227.t1 MTR_2g097463 28.321 399 238 8 198 577 285 654 1.44e-39 154
MS.gene058227.t1 MTR_2g097473 29.668 391 243 9 198 577 284 653 7.76e-39 151
MS.gene058227.t1 MTR_7g062120 27.621 391 264 7 194 578 363 740 2.53e-38 150
MS.gene058227.t1 MTR_7g074650 29.412 374 239 11 207 578 442 792 7.69e-38 149
MS.gene058227.t1 MTR_4g064160 26.076 395 255 7 198 577 311 683 7.69e-38 149
MS.gene058227.t1 MTR_2g097467 28.535 389 250 8 198 577 284 653 1.90e-37 147
MS.gene058227.t1 MTR_4g064120 27.461 386 259 8 192 568 243 616 2.07e-37 147
MS.gene058227.t1 MTR_4g102790 28.052 385 256 10 204 580 293 664 7.96e-37 145
MS.gene058227.t1 MTR_2g026250 26.615 387 265 7 198 578 223 596 9.81e-37 145
MS.gene058227.t1 MTR_5g094450 27.835 388 258 9 200 578 158 532 1.83e-36 143
MS.gene058227.t1 MTR_2g097410 27.342 395 260 8 194 577 362 740 2.21e-36 145
MS.gene058227.t1 MTR_4g064180 26.823 384 264 6 192 568 243 616 5.65e-36 143
MS.gene058227.t1 MTR_4g133660 26.166 386 266 8 198 577 170 542 1.05e-35 141
MS.gene058227.t1 MTR_2g097390 25.000 388 272 6 195 577 267 640 5.17e-35 140
MS.gene058227.t1 MTR_5g097480 27.515 338 223 7 204 533 178 501 9.66e-35 138
MS.gene058227.t1 MTR_3g056110 27.320 388 256 8 200 578 172 542 1.74e-34 137
MS.gene058227.t1 MTR_2g097350 25.515 388 270 6 195 577 266 639 3.51e-34 137
MS.gene058227.t1 MTR_3g022830 26.818 440 230 8 204 579 26 437 7.46e-34 134
MS.gene058227.t1 MTR_2g097310 24.742 388 273 6 195 577 264 637 1.36e-33 136
MS.gene058227.t1 MTR_4g077760 26.943 386 255 9 200 578 190 555 1.59e-33 135
MS.gene058227.t1 MTR_3g072710 26.448 397 273 9 193 577 110 499 5.29e-33 132
MS.gene058227.t1 MTR_4g076020 28.117 377 231 10 207 578 88 429 3.66e-32 129
MS.gene058227.t1 MTR_2g082090 26.302 384 263 8 200 577 209 578 2.56e-31 129
MS.gene058227.t1 MTR_1g069725 25.490 408 272 10 173 577 84 462 4.29e-31 126
MS.gene058227.t1 MTR_3g089055 26.972 393 243 10 204 578 199 565 1.00e-30 127
MS.gene058227.t1 MTR_3g089055 26.972 393 243 10 204 578 204 570 1.02e-30 127
MS.gene058227.t1 MTR_1g029420 25.389 386 258 7 204 578 226 592 4.47e-30 125
MS.gene058227.t1 MTR_2g097380 25.549 364 223 9 219 577 240 560 1.17e-29 123
MS.gene058227.t1 MTR_1g096030 26.506 415 261 12 188 578 65 459 5.96e-29 120
MS.gene058227.t1 MTR_5g058860 26.341 410 263 12 191 577 103 496 2.34e-28 119
MS.gene058227.t1 MTR_4g097080 23.545 378 278 4 204 578 134 503 4.02e-26 112
MS.gene058227.t1 MTR_8g093070 26.980 404 245 15 190 577 126 495 1.85e-23 104
MS.gene058227.t1 MTR_5g015490 24.289 387 270 8 204 578 115 490 1.08e-22 102
MS.gene058227.t1 MTR_4g076140 35.762 151 96 1 428 578 320 469 1.15e-22 101
MS.gene058227.t1 MTR_4g076140 28.720 289 177 9 164 440 1 272 1.22e-18 89.4
MS.gene058227.t1 MTR_7g057230 26.277 411 260 12 185 577 271 656 3.64e-22 101
MS.gene058227.t1 MTR_5g009080 32.895 152 101 1 427 578 328 478 6.15e-22 99.8
MS.gene058227.t1 MTR_5g009080 29.960 247 155 6 183 426 33 264 1.70e-21 98.2
MS.gene058227.t1 MTR_4g026485 25.000 384 260 12 199 578 265 624 3.04e-18 89.0
MS.gene058227.t1 MTR_6g047750 25.564 399 245 14 202 578 294 662 3.19e-18 89.0
MS.gene058227.t1 MTR_2g099110 24.789 355 229 11 143 489 27 351 4.71e-18 87.4
MS.gene058227.t1 MTR_0092s0100 24.147 381 268 9 199 578 371 731 2.16e-16 83.2
MS.gene058227.t1 MTR_0092s0100 24.147 381 268 9 199 578 368 728 2.31e-16 83.2
MS.gene058227.t1 MTR_8g020840 19.802 404 287 9 196 579 163 549 8.16e-15 77.8
MS.gene058227.t1 MTR_2g089100 20.414 338 227 8 204 511 53 378 6.49e-13 71.6
MS.gene058227.t1 MTR_8g077940 24.432 352 236 11 202 545 181 510 2.86e-12 69.7
MS.gene058227.t1 MTR_3g022580 32.743 113 74 1 467 579 74 184 7.61e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058227.t1 AT3G03450 30.711 394 254 7 189 579 169 546 1.62e-56 199
MS.gene058227.t1 AT2G01570 30.357 392 251 6 190 579 211 582 7.86e-56 198
MS.gene058227.t1 AT1G14920 30.612 392 250 6 190 579 159 530 3.47e-54 192
MS.gene058227.t1 AT5G17490 30.077 389 247 8 194 579 151 517 2.80e-53 190
MS.gene058227.t1 AT1G66350 29.820 389 247 6 192 579 144 507 1.30e-52 188
MS.gene058227.t1 AT5G41920 30.227 397 253 10 185 578 25 400 1.55e-44 163
MS.gene058227.t1 AT1G07530 28.822 399 264 6 186 577 379 764 2.89e-43 165
MS.gene058227.t1 AT3G46600 30.729 384 240 9 204 578 5 371 5.45e-43 158
MS.gene058227.t1 AT3G46600 30.357 392 247 9 196 578 205 579 3.60e-42 160
MS.gene058227.t1 AT3G46600 30.357 392 247 9 196 578 173 547 4.10e-42 159
MS.gene058227.t1 AT5G59450 30.867 392 245 11 198 578 222 598 6.60e-42 160
MS.gene058227.t1 AT2G29060 28.133 391 261 7 195 577 276 654 2.22e-40 156
MS.gene058227.t1 AT2G29060 28.133 391 261 7 195 577 313 691 2.47e-40 156
MS.gene058227.t1 AT1G55580 29.268 410 246 10 204 578 45 445 4.16e-40 152
MS.gene058227.t1 AT2G29065 27.225 382 263 7 204 577 253 627 1.69e-39 153
MS.gene058227.t1 AT4G17230 27.461 386 258 8 200 578 155 525 2.53e-39 151
MS.gene058227.t1 AT4G17230 27.461 386 258 8 200 578 155 525 2.53e-39 151
MS.gene058227.t1 AT3G54220 30.108 372 240 9 207 577 297 649 2.48e-38 150
MS.gene058227.t1 AT1G07520 26.289 388 258 8 204 577 155 528 7.05e-38 147
MS.gene058227.t1 AT1G07520 26.289 388 258 8 204 577 155 528 7.05e-38 147
MS.gene058227.t1 AT1G07520 26.289 388 258 8 204 577 320 693 1.05e-37 149
MS.gene058227.t1 AT1G07520 26.289 388 258 8 204 577 319 692 1.17e-37 148
MS.gene058227.t1 AT5G66770 28.070 399 258 10 191 578 204 584 6.90e-37 145
MS.gene058227.t1 AT2G37650 28.571 399 248 11 194 578 326 701 2.74e-35 141
MS.gene058227.t1 AT2G37650 28.571 399 248 11 194 578 338 713 3.20e-35 141
MS.gene058227.t1 AT1G63100 26.566 399 263 9 195 577 269 653 1.04e-34 139
MS.gene058227.t1 AT1G63100 26.566 399 263 9 195 577 244 628 1.08e-34 139
MS.gene058227.t1 AT5G48150 26.946 334 226 6 200 528 119 439 1.33e-33 134
MS.gene058227.t1 AT5G48150 26.946 334 226 6 200 528 119 439 1.33e-33 134
MS.gene058227.t1 AT5G48150 26.946 334 226 6 200 528 119 439 1.33e-33 134
MS.gene058227.t1 AT5G48150 26.946 334 226 6 200 528 119 439 1.33e-33 134
MS.gene058227.t1 AT3G50650 28.022 364 238 8 223 578 195 542 1.13e-32 132
MS.gene058227.t1 AT1G50600 26.221 389 251 10 204 578 160 526 1.21e-32 132
MS.gene058227.t1 AT1G50600 26.632 383 257 8 204 578 231 597 3.07e-32 131
MS.gene058227.t1 AT1G50600 26.632 383 257 8 204 578 235 601 3.12e-32 131
MS.gene058227.t1 AT2G04890 25.397 378 261 6 204 578 54 413 5.22e-32 128
MS.gene058227.t1 AT4G08250 26.471 408 267 13 184 577 88 476 7.64e-28 117
MS.gene058227.t1 AT1G21450 26.554 354 231 9 195 535 214 551 3.85e-23 103
MS.gene058227.t1 AT1G50420 30.597 268 165 8 167 427 15 268 1.70e-22 101
MS.gene058227.t1 AT1G50420 32.278 158 96 3 426 578 328 479 1.15e-20 95.9
MS.gene058227.t1 AT4G37650 21.907 388 285 7 204 578 147 529 1.31e-20 95.9
MS.gene058227.t1 AT2G45160 25.562 356 235 9 230 578 307 639 7.32e-20 94.0
MS.gene058227.t1 AT4G00150 27.638 398 225 18 198 578 203 554 8.20e-20 93.6
MS.gene058227.t1 AT3G49950 24.873 394 259 12 204 577 31 407 7.94e-19 89.4
MS.gene058227.t1 AT3G60630 24.918 305 207 8 285 578 329 622 1.46e-16 83.6

Find 78 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATCCTGTTAATGATTT+TGG 0.104811 2.4:+56490852 MS.gene058227:CDS
GTTCATCCGATGATACATTT+TGG 0.147722 2.4:+56490517 MS.gene058227:CDS
CACATGTGTACAAGCTTTAT+TGG 0.155746 2.4:+56491361 MS.gene058227:CDS
AACCATTACTATAGGATTTA+TGG 0.158396 2.4:-56491765 None:intergenic
AATGGTCATTGTCTTCTTAT+TGG 0.198735 2.4:+56492130 MS.gene058227:CDS
TTTCATTTCACTAAATTTCT+TGG 0.238377 2.4:-56490544 None:intergenic
ATTCTAGAGTCAATGTATTT+TGG 0.251777 2.4:+56491917 MS.gene058227:CDS
CTGTTTCTTTGTCGATTCTT+TGG 0.253189 2.4:-56491205 None:intergenic
TTGATTCTTAATCACCCTTT+TGG 0.265653 2.4:+56490987 MS.gene058227:CDS
GTTGACATATTGAAGAATTA+TGG 0.268072 2.4:+56490786 MS.gene058227:CDS
CAAACAGAAACAGAGTTAAT+TGG 0.268686 2.4:-56492166 None:intergenic
AGATCCTTGCTAACCTCTTC+AGG 0.285294 2.4:-56491281 None:intergenic
GAAACGGTTCACAAAAGATT+TGG 0.292025 2.4:-56491816 None:intergenic
TAATGAACCACACGCTTAAC+CGG 0.323577 2.4:-56491164 None:intergenic
ATAACTATATCGAACGAAAA+AGG 0.330493 2.4:-56491602 None:intergenic
CAATTAACTCTGTTTCTGTT+TGG 0.337936 2.4:+56492167 MS.gene058227:CDS
TCGATTGAAGTGCTTGCATT+AGG 0.355406 2.4:-56491454 None:intergenic
ACTAAACCAAAATCATTAAC+AGG 0.373883 2.4:-56490858 None:intergenic
GTCATTGTCTTCTTATTGGA+TGG 0.423148 2.4:+56492134 MS.gene058227:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.426353 2.4:+56491206 MS.gene058227:CDS
ATGATTGTTTCGAGTTTGTC+TGG 0.434303 2.4:-56491728 None:intergenic
GTCTTTGTATCAAGCTGAAT+TGG 0.435480 2.4:+56492063 MS.gene058227:CDS
GATGTTGGTGATGATACTTT+AGG 0.436439 2.4:-56490620 None:intergenic
AGGATTTCTCCTTCATCATC+AGG 0.437722 2.4:-56490831 None:intergenic
TAATTCTTCAATATGTCAAC+TGG 0.440835 2.4:-56490783 None:intergenic
TCGTACAGCGCGATCATGGC+CGG 0.442099 2.4:-56491308 None:intergenic
AAGGAGATTGGTAGAATGAT+TGG 0.442242 2.4:-56490755 None:intergenic
ATCCAACAATATGCAAAAGA+TGG 0.448011 2.4:+56491244 MS.gene058227:CDS
ATTGATCTCGAAATTCGAAA+AGG 0.450113 2.4:+56491416 MS.gene058227:CDS
AGGAATATTGTGGCTGAAGA+AGG 0.454210 2.4:+56491950 MS.gene058227:CDS
TCGGCGACAACCATTACTAT+AGG 0.455276 2.4:-56491773 None:intergenic
AATATGTCAACTGGTGCTAA+AGG 0.468976 2.4:-56490774 None:intergenic
AAATTCGAAAAGGGTGTCAA+TGG 0.472001 2.4:+56491426 MS.gene058227:CDS
TGTCTTCTTATTGGATGGAA+AGG 0.484516 2.4:+56492139 MS.gene058227:CDS
TCTTGCACATTTGATATGAA+TGG 0.485082 2.4:+56492112 MS.gene058227:CDS
TCTTCGTCGGAATCTGCATC+AGG 0.487554 2.4:+56490963 MS.gene058227:CDS
ATAGAGATCATGAAGCTAGC+TGG 0.499759 2.4:+56490918 MS.gene058227:CDS
ATCTTCGAAGACAGGTAACC+CGG 0.500315 2.4:+56491145 MS.gene058227:CDS
AATATTGAAACTTGACTGAA+TGG 0.501754 2.4:-56491338 None:intergenic
ATCTTCGTACAGCGCGATCA+TGG 0.503332 2.4:-56491312 None:intergenic
GAGTCTTTATCTTCGAAGAC+AGG 0.510353 2.4:+56491137 MS.gene058227:CDS
GGACACAGGTAAAAGATTGA+AGG 0.515574 2.4:+56491556 MS.gene058227:CDS
GATATGATTCTTGTTGATGT+TGG 0.515959 2.4:-56490635 None:intergenic
CGTACAGCGCGATCATGGCC+GGG 0.517805 2.4:-56491307 None:intergenic
TTGATCTCGAAATTCGAAAA+GGG 0.518620 2.4:+56491417 MS.gene058227:CDS
CAACTGGTGCTAAAGGAGAT+TGG 0.521179 2.4:-56490767 None:intergenic
CAAAATCATTAACAGGATGA+AGG 0.525196 2.4:-56490851 None:intergenic
AACCATAAATCCTATAGTAA+TGG 0.541375 2.4:+56491763 MS.gene058227:CDS
TTTGTATCAAGCTGAATTGG+TGG 0.548313 2.4:+56492066 MS.gene058227:CDS
ATTATGATTAGCTTCAATCT+CGG 0.557666 2.4:-56491792 None:intergenic
GGTTTCCCAAAATGTATCAT+CGG 0.559654 2.4:-56490523 None:intergenic
ATCTTTATTTGATCCTGAAG+AGG 0.563321 2.4:+56491268 MS.gene058227:CDS
GTTCAGTTTCAATATAGTTG+AGG 0.565047 2.4:+56490485 MS.gene058227:CDS
TTTCACAAGATTTGGAATGG+TGG 0.567481 2.4:+56492021 MS.gene058227:CDS
CATACAATCATCATCTTCGT+CGG 0.575813 2.4:+56490950 MS.gene058227:CDS
AAGGAGCTGAAAGAAAGAGT+AGG 0.577054 2.4:+56491969 MS.gene058227:CDS
GAAGATCTAGGAGACTATCA+TGG 0.581000 2.4:+56490588 MS.gene058227:CDS
AGGTTATTACCTGATGATGA+AGG 0.583580 2.4:+56490822 MS.gene058227:CDS
AGTGAACCATGTCAAAGTGT+AGG 0.587676 2.4:-56490680 None:intergenic
TGATCCTGAAGAGGTTAGCA+AGG 0.599740 2.4:+56491277 MS.gene058227:CDS
AATGAACCACACGCTTAACC+GGG 0.608506 2.4:-56491163 None:intergenic
TTAGCTTGTGCTGAGAAAGT+CGG 0.613798 2.4:+56491071 MS.gene058227:CDS
TGATAACCTACACTTTGACA+TGG 0.617264 2.4:+56490674 MS.gene058227:CDS
GTTATCTTTAGAAGCTCGAG+TGG 0.620777 2.4:-56491488 None:intergenic
TCATGAAGCTAGCTGGAACA+AGG 0.621798 2.4:+56490925 MS.gene058227:CDS
CACTTCGAAGCATATAGTTG+AGG 0.629728 2.4:+56491535 MS.gene058227:CDS
AGGTAACCCGGTTAAGCGTG+TGG 0.631363 2.4:+56491157 MS.gene058227:CDS
TCATGGAATTAGGAATATTG+TGG 0.639538 2.4:+56491940 MS.gene058227:CDS
ATATTGAAGAATTATGGCAA+AGG 0.640071 2.4:+56490792 MS.gene058227:CDS
AAGATAACAGCTATTGAGAG+CGG 0.640580 2.4:+56491503 MS.gene058227:CDS
ACTCGAAACAATCATGAGAG+TGG 0.651400 2.4:+56491736 MS.gene058227:CDS
GGTTAGCAAGGATCTAAACC+CGG 0.656322 2.4:+56491289 MS.gene058227:CDS
AAGCATATAGTTGAGGACAC+AGG 0.664800 2.4:+56491542 MS.gene058227:CDS
ATTATGGCAAAGGATTCAAG+AGG 0.672484 2.4:+56490802 MS.gene058227:CDS
GTGGCTAAAAGATTTCCTTG+TGG 0.676872 2.4:+56492085 MS.gene058227:CDS
GGAATGTAAAGATTGATGTG+TGG 0.700106 2.4:+56491990 MS.gene058227:CDS
AGATTCGGAAGAAATAGTCG+CGG 0.720614 2.4:+56491667 MS.gene058227:CDS
AATGTGCAAGAACATCCACA+AGG 0.743242 2.4:-56492100 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCATTTCACTAAATTTCT+TGG - chr2.4:56490547-56490566 None:intergenic 20.0%
! AACCATAAATCCTATAGTAA+TGG + chr2.4:56491763-56491782 MS.gene058227:CDS 25.0%
! AACCATTACTATAGGATTTA+TGG - chr2.4:56491768-56491787 None:intergenic 25.0%
! AATATTGAAACTTGACTGAA+TGG - chr2.4:56491341-56491360 None:intergenic 25.0%
! ACTAAACCAAAATCATTAAC+AGG - chr2.4:56490861-56490880 None:intergenic 25.0%
! ATAACTATATCGAACGAAAA+AGG - chr2.4:56491605-56491624 None:intergenic 25.0%
! ATATTGAAGAATTATGGCAA+AGG + chr2.4:56490792-56490811 MS.gene058227:CDS 25.0%
! GTTGACATATTGAAGAATTA+TGG + chr2.4:56490786-56490805 MS.gene058227:CDS 25.0%
! TAATTCTTCAATATGTCAAC+TGG - chr2.4:56490786-56490805 None:intergenic 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.4:56492018-56492037 MS.gene058227:CDS 25.0%
!! TAAAGTGGAAGAATTCTTTT+AGG - chr2.4:56490720-56490739 None:intergenic 25.0%
!!! AGATCTTTTCAAGATAGATT+CGG + chr2.4:56491652-56491671 MS.gene058227:CDS 25.0%
!!! ATTATGATTAGCTTCAATCT+CGG - chr2.4:56491795-56491814 None:intergenic 25.0%
!!! ATTCTAGAGTCAATGTATTT+TGG + chr2.4:56491917-56491936 MS.gene058227:CDS 25.0%
!!! TGTTTTGAGAATTGTATGAA+AGG + chr2.4:56491875-56491894 MS.gene058227:CDS 25.0%
AATGGTCATTGTCTTCTTAT+TGG + chr2.4:56492130-56492149 MS.gene058227:CDS 30.0%
ACGAGAAAGAAAAACCAAAA+GGG - chr2.4:56491004-56491023 None:intergenic 30.0%
ATCCAACAATATGCAAAAGA+TGG + chr2.4:56491244-56491263 MS.gene058227:CDS 30.0%
ATTGATCTCGAAATTCGAAA+AGG + chr2.4:56491416-56491435 MS.gene058227:CDS 30.0%
CAAAATCATTAACAGGATGA+AGG - chr2.4:56490854-56490873 None:intergenic 30.0%
CAAACAGAAACAGAGTTAAT+TGG - chr2.4:56492169-56492188 None:intergenic 30.0%
GATATGATTCTTGTTGATGT+TGG - chr2.4:56490638-56490657 None:intergenic 30.0%
GTTCAGTTTCAATATAGTTG+AGG + chr2.4:56490485-56490504 MS.gene058227:CDS 30.0%
TCATGGAATTAGGAATATTG+TGG + chr2.4:56491940-56491959 MS.gene058227:CDS 30.0%
TCCACTTTATGACACAAAAA+AGG + chr2.4:56490731-56490750 MS.gene058227:CDS 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.4:56492112-56492131 MS.gene058227:CDS 30.0%
TTGATCTCGAAATTCGAAAA+GGG + chr2.4:56491417-56491436 MS.gene058227:CDS 30.0%
! ATCTTTATTTGATCCTGAAG+AGG + chr2.4:56491268-56491287 MS.gene058227:CDS 30.0%
! CTTCATCCTGTTAATGATTT+TGG + chr2.4:56490852-56490871 MS.gene058227:CDS 30.0%
! TTCATCCGATGATACATTTT+GGG + chr2.4:56490518-56490537 MS.gene058227:CDS 30.0%
! TTCCATCTTTTGCATATTGT+TGG - chr2.4:56491249-56491268 None:intergenic 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.4:56490987-56491006 MS.gene058227:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.4:56491835-56491854 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.4:56492013-56492032 MS.gene058227:CDS 30.0%
!! CAATTAACTCTGTTTCTGTT+TGG + chr2.4:56492167-56492186 MS.gene058227:CDS 30.0%
!!! TCCTTTTTTGTGTCATAAAG+TGG - chr2.4:56490735-56490754 None:intergenic 30.0%
!!! TGTATTTTGGTCATGGAATT+AGG + chr2.4:56491930-56491949 MS.gene058227:CDS 30.0%
AAGATAACAGCTATTGAGAG+CGG + chr2.4:56491503-56491522 MS.gene058227:CDS 35.0%
AAGGAGATTGGTAGAATGAT+TGG - chr2.4:56490758-56490777 None:intergenic 35.0%
ATTATGGCAAAGGATTCAAG+AGG + chr2.4:56490802-56490821 MS.gene058227:CDS 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.4:56491206-56491225 MS.gene058227:CDS 35.0%
CACGAGAAAGAAAAACCAAA+AGG - chr2.4:56491005-56491024 None:intergenic 35.0%
CATACAATCATCATCTTCGT+CGG + chr2.4:56490950-56490969 MS.gene058227:CDS 35.0%
GAAACGGTTCACAAAAGATT+TGG - chr2.4:56491819-56491838 None:intergenic 35.0%
GGAATGTAAAGATTGATGTG+TGG + chr2.4:56491990-56492009 MS.gene058227:CDS 35.0%
GGTTTCCCAAAATGTATCAT+CGG - chr2.4:56490526-56490545 None:intergenic 35.0%
GTCATTGTCTTCTTATTGGA+TGG + chr2.4:56492134-56492153 MS.gene058227:CDS 35.0%
GTCTTTGTATCAAGCTGAAT+TGG + chr2.4:56492063-56492082 MS.gene058227:CDS 35.0%
TGATAACCTACACTTTGACA+TGG + chr2.4:56490674-56490693 MS.gene058227:CDS 35.0%
TGTCTTCTTATTGGATGGAA+AGG + chr2.4:56492139-56492158 MS.gene058227:CDS 35.0%
TTTGTATCAAGCTGAATTGG+TGG + chr2.4:56492066-56492085 MS.gene058227:CDS 35.0%
! AATATGTCAACTGGTGCTAA+AGG - chr2.4:56490777-56490796 None:intergenic 35.0%
! AGGTTATTACCTGATGATGA+AGG + chr2.4:56490822-56490841 MS.gene058227:CDS 35.0%
! CACATGTGTACAAGCTTTAT+TGG + chr2.4:56491361-56491380 MS.gene058227:CDS 35.0%
! GTTCATCCGATGATACATTT+TGG + chr2.4:56490517-56490536 MS.gene058227:CDS 35.0%
! TTTCACAAGATTTGGAATGG+TGG + chr2.4:56492021-56492040 MS.gene058227:CDS 35.0%
!! AAATTCGAAAAGGGTGTCAA+TGG + chr2.4:56491426-56491445 MS.gene058227:CDS 35.0%
!! ATGATTGTTTCGAGTTTGTC+TGG - chr2.4:56491731-56491750 None:intergenic 35.0%
!! CTGTTTCTTTGTCGATTCTT+TGG - chr2.4:56491208-56491227 None:intergenic 35.0%
!! GATGTTGGTGATGATACTTT+AGG - chr2.4:56490623-56490642 None:intergenic 35.0%
!!! GAGTCAATGTATTTTGGTCA+TGG + chr2.4:56491923-56491942 MS.gene058227:CDS 35.0%
AAGCATATAGTTGAGGACAC+AGG + chr2.4:56491542-56491561 MS.gene058227:CDS 40.0%
AAGGAGCTGAAAGAAAGAGT+AGG + chr2.4:56491969-56491988 MS.gene058227:CDS 40.0%
AATGTGCAAGAACATCCACA+AGG - chr2.4:56492103-56492122 None:intergenic 40.0%
ACTCGAAACAATCATGAGAG+TGG + chr2.4:56491736-56491755 MS.gene058227:CDS 40.0%
AGATTCGGAAGAAATAGTCG+CGG + chr2.4:56491667-56491686 MS.gene058227:CDS 40.0%
AGGAATATTGTGGCTGAAGA+AGG + chr2.4:56491950-56491969 MS.gene058227:CDS 40.0%
AGGATTTCTCCTTCATCATC+AGG - chr2.4:56490834-56490853 None:intergenic 40.0%
AGTGAACCATGTCAAAGTGT+AGG - chr2.4:56490683-56490702 None:intergenic 40.0%
ATAGAGATCATGAAGCTAGC+TGG + chr2.4:56490918-56490937 MS.gene058227:CDS 40.0%
CACTTCGAAGCATATAGTTG+AGG + chr2.4:56491535-56491554 MS.gene058227:CDS 40.0%
GAAGATCTAGGAGACTATCA+TGG + chr2.4:56490588-56490607 MS.gene058227:CDS 40.0%
GAGTCTTTATCTTCGAAGAC+AGG + chr2.4:56491137-56491156 MS.gene058227:CDS 40.0%
GGACACAGGTAAAAGATTGA+AGG + chr2.4:56491556-56491575 MS.gene058227:CDS 40.0%
GTGGCTAAAAGATTTCCTTG+TGG + chr2.4:56492085-56492104 MS.gene058227:CDS 40.0%
TAATGAACCACACGCTTAAC+CGG - chr2.4:56491167-56491186 None:intergenic 40.0%
! GTTATCTTTAGAAGCTCGAG+TGG - chr2.4:56491491-56491510 None:intergenic 40.0%
! TCGATTGAAGTGCTTGCATT+AGG - chr2.4:56491457-56491476 None:intergenic 40.0%
! TTTTCTGCAGCAGAAGATCT+AGG + chr2.4:56490576-56490595 MS.gene058227:CDS 40.0%
!! TTAGCTTGTGCTGAGAAAGT+CGG + chr2.4:56491071-56491090 MS.gene058227:CDS 40.0%
AATGAACCACACGCTTAACC+GGG - chr2.4:56491166-56491185 None:intergenic 45.0%
AGATCCTTGCTAACCTCTTC+AGG - chr2.4:56491284-56491303 None:intergenic 45.0%
ATCTTCGAAGACAGGTAACC+CGG + chr2.4:56491145-56491164 MS.gene058227:CDS 45.0%
GGTTAGCAAGGATCTAAACC+CGG + chr2.4:56491289-56491308 MS.gene058227:CDS 45.0%
TCATGAAGCTAGCTGGAACA+AGG + chr2.4:56490925-56490944 MS.gene058227:CDS 45.0%
TCGGCGACAACCATTACTAT+AGG - chr2.4:56491776-56491795 None:intergenic 45.0%
TGATCCTGAAGAGGTTAGCA+AGG + chr2.4:56491277-56491296 MS.gene058227:CDS 45.0%
! CAACTGGTGCTAAAGGAGAT+TGG - chr2.4:56490770-56490789 None:intergenic 45.0%
ATCTTCGTACAGCGCGATCA+TGG - chr2.4:56491315-56491334 None:intergenic 50.0%
TCTTCGTCGGAATCTGCATC+AGG + chr2.4:56490963-56490982 MS.gene058227:CDS 50.0%
AGGTAACCCGGTTAAGCGTG+TGG + chr2.4:56491157-56491176 MS.gene058227:CDS 55.0%
TCGTACAGCGCGATCATGGC+CGG - chr2.4:56491311-56491330 None:intergenic 60.0%
CGTACAGCGCGATCATGGCC+GGG - chr2.4:56491310-56491329 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 56490459 56492201 56490459 ID=MS.gene058227
chr2.4 mRNA 56490459 56492201 56490459 ID=MS.gene058227.t1;Parent=MS.gene058227
chr2.4 exon 56490459 56492201 56490459 ID=MS.gene058227.t1.exon1;Parent=MS.gene058227.t1
chr2.4 CDS 56490459 56492201 56490459 ID=cds.MS.gene058227.t1;Parent=MS.gene058227.t1
Gene Sequence

>MS.gene058227

ATGGAAAACTTGTGCAATTTTGATGAGTTCAGTTTCAATATAGTTGAGGATAAACTTAGTTCATCCGATGATACATTTTGGGAAACCAAGAAATTTAGTGAAATGAAAAATGTTAAATTTTCTGCAGCAGAAGATCTAGGAGACTATCATGGAATTGAGTTCCTAAAGTATCATCACCAACATCAACAAGAATCATATCAAGATTATGAAACTTTTGATAACCTACACTTTGACATGGTTCACTTTGATGAACAATGTCCTAAAAGAATTCTTCCACTTTATGACACAAAAAAGGACCAATCATTCTACCAATCTCCTTTAGCACCAGTTGACATATTGAAGAATTATGGCAAAGGATTCAAGAGGTTATTACCTGATGATGAAGGAGAAATCCTTCATCCTGTTAATGATTTTGGTTTAGTCACAAATAATGAAAATGAGAAAAAGTTGTTGTCAACTATAGAGATCATGAAGCTAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGGAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTCTTTCTCGTGTTTATCTGATGAAGAGAAAGAAGATGTTTCGCTTGCTGAATCACTCTTAGCTTGTGCTGAGAAAGTCGGATATCAACAATATGAACGCGCTAGAAAATTGTTGTCACAAATCGAGTCTTTATCTTCGAAGACAGGTAACCCGGTTAAGCGTGTGGTTCATTATTTTGCTGAAGCTCTTCGCCAAAGAATCGACAAAGAAACAGGACGATTTTCTGTTTCATCCAACAATATGCAAAAGATGGAATCTTTATTTGATCCTGAAGAGGTTAGCAAGGATCTAAACCCGGCCATGATCGCGCTGTACGAAGATCTTCCATTCAGTCAAGTTTCAATATTCACATGTGTACAAGCTTTATTGGAAAATGTAAATGATGCAAAGAAGATTCATGTTATTGATCTCGAAATTCGAAAAGGGTGTCAATGGACAATCCTAATGCAAGCACTTCAATCGAGAAACGAATGTCCACTCGAGCTTCTAAAGATAACAGCTATTGAGAGCGGAAACTCAGACACTTCGAAGCATATAGTTGAGGACACAGGTAAAAGATTGAAGGATTTTGCGCAAAGTTTGAACATACCTTTTTCGTTCGATATAGTTATTGTATCTGATTTGTTAAATTTAAGAGAAGATCTTTTCAAGATAGATTCGGAAGAAATAGTCGCGGTTTATTCTCAATTTGCGCTTAAAAGCAAGATTCAACAACCAGACAAACTCGAAACAATCATGAGAGTGGTTAGAACCATAAATCCTATAGTAATGGTTGTCGCCGAGATTGAAGCTAATCATAATTCCAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCATACTTTGATTGTTTTGAGAATTGTATGAAAGGTGATGAGAAAAACAGATTTATTCTAGAGTCAATGTATTTTGGTCATGGAATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGGAATGTAAAGATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTGGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTGGTGGCTAAAAGATTTCCTTGTGGATGTTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTATTGGATGGAAAGGAACACCAATTAACTCTGTTTCTGTTTGGAAATTCATTTGA
Protein sequence

>MS.gene058227.t1

MENLCNFDEFSFNIVEDKLSSSDDTFWETKKFSEMKNVKFSAAEDLGDYHGIEFLKYHHQHQQESYQDYETFDNLHFDMVHFDEQCPKRILPLYDTKKDQSFYQSPLAPVDILKNYGKGFKRLLPDDEGEILHPVNDFGLVTNNENEKKLLSTIEIMKLAGTRFIQSSSSSESASGLILNHPFGFSFSCLSDEEKEDVSLAESLLACAEKVGYQQYERARKLLSQIESLSSKTGNPVKRVVHYFAEALRQRIDKETGRFSVSSNNMQKMESLFDPEEVSKDLNPAMIALYEDLPFSQVSIFTCVQALLENVNDAKKIHVIDLEIRKGCQWTILMQALQSRNECPLELLKITAIESGNSDTSKHIVEDTGKRLKDFAQSLNIPFSFDIVIVSDLLNLREDLFKIDSEEIVAVYSQFALKSKIQQPDKLETIMRVVRTINPIVMVVAEIEANHNSKSFVNRFIEALFYFSAYFDCFENCMKGDEKNRFILESMYFGHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFPCGCSCTFDMNGHCLLIGWKGTPINSVSVWKFI