AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070232

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070232.t1 MTR_2g034250 89.983 599 28 4 1 579 1 587 0.0 1071
MS.gene070232.t1 MTR_2g034260 78.182 605 87 9 1 579 1 586 0.0 922
MS.gene070232.t1 MTR_2g034280 74.257 606 100 10 1 579 1 577 0.0 862
MS.gene070232.t1 MTR_7g109580 45.570 474 236 6 107 579 104 556 4.93e-133 399
MS.gene070232.t1 MTR_1g086970 45.527 503 243 11 84 579 2 480 3.14e-132 395
MS.gene070232.t1 MTR_3g065980 28.372 430 273 7 165 578 131 541 2.80e-56 198
MS.gene070232.t1 MTR_7g027190 30.326 399 250 8 192 577 290 673 4.56e-46 172
MS.gene070232.t1 MTR_4g104020 29.534 386 254 7 199 577 146 520 4.89e-44 164
MS.gene070232.t1 MTR_8g442410 30.808 396 243 11 195 577 148 525 6.23e-44 164
MS.gene070232.t1 MTR_4g064150 29.487 390 252 9 197 576 356 732 7.84e-42 161
MS.gene070232.t1 MTR_4g064200 29.124 388 250 10 197 576 279 649 1.48e-40 156
MS.gene070232.t1 MTR_2g097463 28.205 390 250 8 197 576 285 654 4.16e-39 152
MS.gene070232.t1 MTR_2g097473 29.771 393 240 10 197 576 284 653 1.24e-38 151
MS.gene070232.t1 MTR_4g102790 28.831 385 253 11 203 579 293 664 2.23e-38 150
MS.gene070232.t1 MTR_7g062120 28.021 389 257 8 197 577 367 740 2.69e-38 150
MS.gene070232.t1 MTR_4g064160 27.132 387 261 7 197 576 311 683 1.27e-37 148
MS.gene070232.t1 MTR_5g094450 27.577 388 259 9 199 577 158 532 1.36e-37 146
MS.gene070232.t1 MTR_2g097467 28.900 391 246 9 197 576 284 653 2.46e-37 147
MS.gene070232.t1 MTR_2g026250 26.615 387 265 7 197 577 223 596 2.58e-37 146
MS.gene070232.t1 MTR_7g074650 29.679 374 238 12 206 577 442 792 3.77e-37 147
MS.gene070232.t1 MTR_4g064120 28.272 382 253 8 195 567 247 616 4.33e-37 146
MS.gene070232.t1 MTR_4g133660 25.377 398 278 8 185 576 158 542 4.82e-37 145
MS.gene070232.t1 MTR_3g022830 27.045 440 229 8 203 578 26 437 1.10e-35 139
MS.gene070232.t1 MTR_2g097410 27.877 391 255 8 197 576 366 740 1.17e-35 142
MS.gene070232.t1 MTR_4g064180 28.158 380 256 7 195 567 247 616 1.19e-35 142
MS.gene070232.t1 MTR_2g097390 25.773 388 269 6 194 576 267 640 1.56e-35 142
MS.gene070232.t1 MTR_5g097480 26.234 385 256 10 203 577 178 544 2.08e-35 140
MS.gene070232.t1 MTR_3g056110 27.461 386 258 8 199 577 172 542 3.65e-35 139
MS.gene070232.t1 MTR_3g072710 27.068 399 272 9 190 576 108 499 6.94e-35 138
MS.gene070232.t1 MTR_2g097350 26.289 388 267 6 194 576 266 639 9.51e-35 139
MS.gene070232.t1 MTR_4g077760 27.062 388 252 10 199 577 190 555 2.31e-34 137
MS.gene070232.t1 MTR_2g097310 25.515 388 270 6 194 576 264 637 3.66e-34 137
MS.gene070232.t1 MTR_1g069725 25.553 407 272 10 173 576 84 462 4.36e-33 132
MS.gene070232.t1 MTR_2g082090 25.974 385 263 9 199 576 209 578 7.87e-32 130
MS.gene070232.t1 MTR_1g029420 25.000 380 267 6 203 577 226 592 1.46e-31 129
MS.gene070232.t1 MTR_4g076020 27.586 377 233 10 206 577 88 429 1.96e-31 127
MS.gene070232.t1 MTR_3g089055 26.221 389 251 9 203 577 199 565 1.39e-30 126
MS.gene070232.t1 MTR_3g089055 26.221 389 251 9 203 577 204 570 1.48e-30 126
MS.gene070232.t1 MTR_5g058860 26.485 404 262 11 192 576 109 496 5.96e-30 124
MS.gene070232.t1 MTR_2g097380 26.099 364 221 9 218 576 240 560 1.15e-29 123
MS.gene070232.t1 MTR_1g096030 25.366 410 262 12 192 577 70 459 5.39e-27 114
MS.gene070232.t1 MTR_4g097080 22.751 378 281 4 203 577 134 503 1.01e-25 111
MS.gene070232.t1 MTR_8g093070 27.136 398 240 15 195 576 132 495 1.04e-24 108
MS.gene070232.t1 MTR_4g076140 38.065 155 87 4 427 577 320 469 2.17e-24 107
MS.gene070232.t1 MTR_4g076140 28.519 270 169 8 174 439 23 272 8.57e-19 89.7
MS.gene070232.t1 MTR_5g009080 35.256 156 92 4 426 577 328 478 7.81e-23 102
MS.gene070232.t1 MTR_5g009080 29.317 249 157 6 179 425 33 264 5.75e-22 99.8
MS.gene070232.t1 MTR_5g015490 23.514 387 273 8 203 577 115 490 6.71e-22 99.8
MS.gene070232.t1 MTR_7g057230 25.964 389 250 11 201 576 293 656 6.08e-21 97.4
MS.gene070232.t1 MTR_2g099110 25.212 353 229 10 143 488 27 351 5.63e-20 93.2
MS.gene070232.t1 MTR_6g047750 25.063 399 247 14 201 577 294 662 1.72e-18 89.7
MS.gene070232.t1 MTR_4g026485 24.675 385 260 12 198 577 265 624 1.12e-17 87.0
MS.gene070232.t1 MTR_8g020840 20.297 404 285 9 195 578 163 549 1.50e-16 83.2
MS.gene070232.t1 MTR_0092s0100 23.885 381 269 9 198 577 371 731 1.13e-15 80.9
MS.gene070232.t1 MTR_0092s0100 23.885 381 269 9 198 577 368 728 1.23e-15 80.9
MS.gene070232.t1 MTR_2g089100 20.944 339 224 8 203 510 53 378 6.08e-13 71.6
MS.gene070232.t1 MTR_8g077940 23.377 385 263 12 201 577 181 541 1.59e-12 70.5
MS.gene070232.t1 MTR_3g022580 33.628 113 73 1 466 578 74 184 1.99e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070232.t1 AT3G03450 30.556 396 256 7 186 578 167 546 4.08e-57 201
MS.gene070232.t1 AT2G01570 29.897 388 250 6 193 578 215 582 1.01e-55 198
MS.gene070232.t1 AT5G17490 30.077 389 247 8 193 578 151 517 1.43e-54 193
MS.gene070232.t1 AT1G66350 30.203 394 249 6 186 578 139 507 3.37e-54 192
MS.gene070232.t1 AT1G14920 29.897 388 250 6 193 578 163 530 6.83e-53 189
MS.gene070232.t1 AT3G46600 30.990 384 239 9 203 577 5 371 3.40e-44 161
MS.gene070232.t1 AT1G07530 29.574 399 260 7 186 576 379 764 9.67e-44 167
MS.gene070232.t1 AT3G46600 30.534 393 247 9 194 577 204 579 1.20e-43 164
MS.gene070232.t1 AT3G46600 30.534 393 247 9 194 577 172 547 1.90e-43 163
MS.gene070232.t1 AT5G59450 31.282 390 246 10 197 577 222 598 1.96e-43 164
MS.gene070232.t1 AT5G41920 30.334 389 247 10 192 577 33 400 1.08e-42 158
MS.gene070232.t1 AT2G29060 28.796 382 252 7 203 576 285 654 1.40e-40 157
MS.gene070232.t1 AT2G29060 28.796 382 252 7 203 576 322 691 1.94e-40 156
MS.gene070232.t1 AT1G55580 29.756 410 244 10 203 577 45 445 2.63e-40 152
MS.gene070232.t1 AT4G17230 27.979 386 256 8 199 577 155 525 3.45e-40 154
MS.gene070232.t1 AT4G17230 27.979 386 256 8 199 577 155 525 3.45e-40 154
MS.gene070232.t1 AT2G29065 27.487 382 262 7 203 576 253 627 7.32e-40 154
MS.gene070232.t1 AT1G07520 27.320 388 254 9 203 576 155 528 3.34e-38 148
MS.gene070232.t1 AT1G07520 27.320 388 254 9 203 576 155 528 3.34e-38 148
MS.gene070232.t1 AT1G07520 27.320 388 254 9 203 576 319 692 7.16e-38 149
MS.gene070232.t1 AT1G07520 27.320 388 254 9 203 576 320 693 7.76e-38 149
MS.gene070232.t1 AT3G54220 29.301 372 243 9 206 576 297 649 2.04e-36 144
MS.gene070232.t1 AT1G63100 26.933 401 263 9 192 576 267 653 2.21e-36 144
MS.gene070232.t1 AT1G63100 26.933 401 263 9 192 576 242 628 2.48e-36 144
MS.gene070232.t1 AT5G66770 28.463 397 253 11 193 577 207 584 6.57e-36 142
MS.gene070232.t1 AT5G48150 25.323 387 266 9 199 577 119 490 6.51e-34 135
MS.gene070232.t1 AT5G48150 25.323 387 266 9 199 577 119 490 6.51e-34 135
MS.gene070232.t1 AT5G48150 25.323 387 266 9 199 577 119 490 6.51e-34 135
MS.gene070232.t1 AT5G48150 25.323 387 266 9 199 577 119 490 6.51e-34 135
MS.gene070232.t1 AT2G37650 27.877 391 253 9 197 577 342 713 8.24e-34 137
MS.gene070232.t1 AT2G37650 27.877 391 253 9 197 577 330 701 9.12e-34 137
MS.gene070232.t1 AT1G50600 26.110 383 259 8 203 577 160 526 1.23e-33 135
MS.gene070232.t1 AT2G04890 25.197 381 258 9 203 577 54 413 1.46e-33 132
MS.gene070232.t1 AT1G50600 26.110 383 259 8 203 577 235 601 3.19e-33 134
MS.gene070232.t1 AT1G50600 26.110 383 259 8 203 577 231 597 3.37e-33 134
MS.gene070232.t1 AT3G50650 27.869 366 236 9 222 577 195 542 1.97e-32 131
MS.gene070232.t1 AT4G08250 27.000 400 259 13 191 576 96 476 2.60e-29 122
MS.gene070232.t1 AT1G21450 24.870 386 261 10 203 577 226 593 2.10e-24 108
MS.gene070232.t1 AT1G50420 30.597 268 164 8 167 426 15 268 4.78e-23 103
MS.gene070232.t1 AT1G50420 32.911 158 95 3 425 577 328 479 3.32e-21 97.4
MS.gene070232.t1 AT4G37650 22.222 414 297 9 178 577 127 529 1.76e-20 95.5
MS.gene070232.t1 AT3G49950 25.127 394 258 12 203 576 31 407 2.16e-20 94.4
MS.gene070232.t1 AT2G45160 25.210 357 235 10 229 577 307 639 4.72e-19 91.7
MS.gene070232.t1 AT4G00150 27.318 399 225 19 197 577 203 554 8.55e-19 90.5
MS.gene070232.t1 AT5G52510 23.308 399 259 11 201 577 267 640 3.59e-18 88.6
MS.gene070232.t1 AT3G60630 24.503 302 212 8 284 577 329 622 8.44e-16 81.3
MS.gene070232.t1 AT3G13840 24.667 300 196 10 192 478 137 419 2.72e-11 66.6

Find 75 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATCCTGTTAATGATTT+TGG 0.104811 2.2:+54670640 MS.gene070232:CDS
GTTCATCCGATGATACATTT+TGG 0.147722 2.2:+54670305 MS.gene070232:CDS
CACATGTGTACAAGCTTTAT+TGG 0.155746 2.2:+54671146 MS.gene070232:CDS
AACCATTACTATAGGATTTA+TGG 0.158396 2.2:-54671550 None:intergenic
AATGGTCATTGTCTTCTTAT+TGG 0.198735 2.2:+54671915 MS.gene070232:CDS
TTTCATTTCACTAAATTTCT+TGG 0.238377 2.2:-54670332 None:intergenic
ATTCTAGAGTCAATGTATTT+TGG 0.251777 2.2:+54671702 MS.gene070232:CDS
CTGTTTCTTTGTCGATTCTT+TGG 0.253189 2.2:-54670990 None:intergenic
TTGATTCTTAATCACCCTTT+TGG 0.265653 2.2:+54670775 MS.gene070232:CDS
GTTGACATATTGAAGAATTA+TGG 0.268072 2.2:+54670574 MS.gene070232:CDS
CAAACAGAAACAGAGTTAAT+TGG 0.268686 2.2:-54671951 None:intergenic
AGATCCTTGCTAACCTCTTC+AGG 0.285294 2.2:-54671066 None:intergenic
GAAACGGTTCACAAAAGATT+TGG 0.292025 2.2:-54671601 None:intergenic
TAATGAACCACACGCTTAAC+CGG 0.323577 2.2:-54670949 None:intergenic
ATAACTATATCGAACGAAAA+AGG 0.330493 2.2:-54671387 None:intergenic
CAATTAACTCTGTTTCTGTT+TGG 0.337936 2.2:+54671952 MS.gene070232:CDS
TCGATTGAAGTGCTTGCATT+AGG 0.355406 2.2:-54671239 None:intergenic
ACTAAACCAAAATCATTAAC+AGG 0.373883 2.2:-54670646 None:intergenic
TTTGTATCAAGCTGAATTAG+TGG 0.394060 2.2:+54671851 MS.gene070232:CDS
GTCATTGTCTTCTTATTGGA+TGG 0.423148 2.2:+54671919 MS.gene070232:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.426353 2.2:+54670991 MS.gene070232:CDS
ATGATTGTTTCGAGTTTGTC+TGG 0.434303 2.2:-54671513 None:intergenic
GATGTTGGTGATGATACTTT+AGG 0.436439 2.2:-54670408 None:intergenic
AGGATTTCTCCTTCATCATC+AGG 0.437722 2.2:-54670619 None:intergenic
GGAATGTAAAGATTGATGTT+TGG 0.438820 2.2:+54671775 MS.gene070232:CDS
TAATTCTTCAATATGTCAAC+TGG 0.440835 2.2:-54670571 None:intergenic
TCGTACAGCGCGATCATGGC+CGG 0.442099 2.2:-54671093 None:intergenic
AAGGAGATTGGTAGAATGAT+TGG 0.442242 2.2:-54670543 None:intergenic
ATCCAACAATATGCAAAAGA+TGG 0.448011 2.2:+54671029 MS.gene070232:CDS
ATTGATCTCGAAATTCGAAA+AGG 0.450113 2.2:+54671201 MS.gene070232:CDS
AGGAATATTGTGGCTGAAGA+AGG 0.454210 2.2:+54671735 MS.gene070232:CDS
TCGGCGACAACCATTACTAT+AGG 0.455276 2.2:-54671558 None:intergenic
AATATGTCAACTGGTGCTAA+AGG 0.468976 2.2:-54670562 None:intergenic
AAATTCGAAAAGGGTGTCAA+TGG 0.472001 2.2:+54671211 MS.gene070232:CDS
TGTCTTCTTATTGGATGGAA+AGG 0.484516 2.2:+54671924 MS.gene070232:CDS
GCTTGCACATTTGATATGAA+TGG 0.486563 2.2:+54671897 MS.gene070232:CDS
TCTTCGTCGGAATCTGCATC+AGG 0.487554 2.2:+54670751 MS.gene070232:CDS
ATAGAGATCATGAAGCTAGC+TGG 0.499759 2.2:+54670706 MS.gene070232:CDS
ATCTTCGAAGACAGGTAACC+CGG 0.500315 2.2:+54670930 MS.gene070232:CDS
AATATTGAAACTTGACTGAA+TGG 0.501754 2.2:-54671123 None:intergenic
ATCTTCGTACAGCGCGATCA+TGG 0.503332 2.2:-54671097 None:intergenic
GAGTCTTTATCTTCGAAGAC+AGG 0.510353 2.2:+54670922 MS.gene070232:CDS
GGACACTGGTAAAAGATTGA+AGG 0.513135 2.2:+54671341 MS.gene070232:CDS
GATATGATTCTTGTTGATGT+TGG 0.515959 2.2:-54670423 None:intergenic
CGTACAGCGCGATCATGGCC+GGG 0.517805 2.2:-54671092 None:intergenic
TTGATCTCGAAATTCGAAAA+GGG 0.518620 2.2:+54671202 MS.gene070232:CDS
CAACTGGTGCTAAAGGAGAT+TGG 0.521179 2.2:-54670555 None:intergenic
CAAAATCATTAACAGGATGA+AGG 0.525196 2.2:-54670639 None:intergenic
AACCATAAATCCTATAGTAA+TGG 0.541375 2.2:+54671548 MS.gene070232:CDS
GGTTTCCCAAAATGTATCAT+CGG 0.559654 2.2:-54670311 None:intergenic
ATCTTTATTTGATCCTGAAG+AGG 0.563321 2.2:+54671053 MS.gene070232:CDS
GTTCAGTTTCAATATAGTTG+AGG 0.565047 2.2:+54670273 MS.gene070232:CDS
TTTCACAAGATTTGGAATGG+TGG 0.567481 2.2:+54671806 MS.gene070232:CDS
CATACAATCATCATCTTCGT+CGG 0.575813 2.2:+54670738 MS.gene070232:CDS
AAGGAGCTGAAAGAAAGAGT+AGG 0.577054 2.2:+54671754 MS.gene070232:CDS
GAAGATCTAGGAGACTATCA+TGG 0.581000 2.2:+54670376 MS.gene070232:CDS
AGGTTATTACCTGATGATGA+AGG 0.583580 2.2:+54670610 MS.gene070232:CDS
AGTGAACCATGTCAAAGTGT+AGG 0.587676 2.2:-54670468 None:intergenic
GTGGCTAAAAGATTTGCTTG+TGG 0.588267 2.2:+54671870 MS.gene070232:CDS
ATTGTGATTAGCTTCAATCT+CGG 0.594753 2.2:-54671577 None:intergenic
TGATCCTGAAGAGGTTAGCA+AGG 0.599740 2.2:+54671062 MS.gene070232:CDS
AATGAACCACACGCTTAACC+GGG 0.608506 2.2:-54670948 None:intergenic
TTAGCTTGTGCTGAGAAAGT+CGG 0.613798 2.2:+54670856 MS.gene070232:CDS
TGATAACCTACACTTTGACA+TGG 0.617264 2.2:+54670462 MS.gene070232:CDS
GTTATCTTTAGAAGCTCGAG+TGG 0.620777 2.2:-54671273 None:intergenic
TCATGAAGCTAGCTGGAACA+AGG 0.621798 2.2:+54670713 MS.gene070232:CDS
CACTTCGAAGCATATAGTTG+AGG 0.629728 2.2:+54671320 MS.gene070232:CDS
AGGTAACCCGGTTAAGCGTG+TGG 0.631363 2.2:+54670942 MS.gene070232:CDS
TCATGGAATTAGGAATATTG+TGG 0.639538 2.2:+54671725 MS.gene070232:CDS
ATATTGAAGAATTATGGCAA+AGG 0.640071 2.2:+54670580 MS.gene070232:CDS
AAGATAACAGCTATTGAGAG+CGG 0.640580 2.2:+54671288 MS.gene070232:CDS
AAGCATATAGTTGAGGACAC+TGG 0.647935 2.2:+54671327 MS.gene070232:CDS
GGTTAGCAAGGATCTAAACC+CGG 0.656322 2.2:+54671074 MS.gene070232:CDS
ATTATGGCAAAGGATTCAAG+AGG 0.672484 2.2:+54670590 MS.gene070232:CDS
AGATTCGGAAGAAACAGTCG+CGG 0.728949 2.2:+54671452 MS.gene070232:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCATTTCACTAAATTTCT+TGG - chr2.2:54670335-54670354 None:intergenic 20.0%
! AACCATAAATCCTATAGTAA+TGG + chr2.2:54671548-54671567 MS.gene070232:CDS 25.0%
! AACCATTACTATAGGATTTA+TGG - chr2.2:54671553-54671572 None:intergenic 25.0%
! AATATTGAAACTTGACTGAA+TGG - chr2.2:54671126-54671145 None:intergenic 25.0%
! ACTAAACCAAAATCATTAAC+AGG - chr2.2:54670649-54670668 None:intergenic 25.0%
! ATAACTATATCGAACGAAAA+AGG - chr2.2:54671390-54671409 None:intergenic 25.0%
! ATATTGAAGAATTATGGCAA+AGG + chr2.2:54670580-54670599 MS.gene070232:CDS 25.0%
! GTTGACATATTGAAGAATTA+TGG + chr2.2:54670574-54670593 MS.gene070232:CDS 25.0%
! TAATTCTTCAATATGTCAAC+TGG - chr2.2:54670574-54670593 None:intergenic 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.2:54671803-54671822 MS.gene070232:CDS 25.0%
!! TAAAGTGGAAGAATTCTTTT+AGG - chr2.2:54670508-54670527 None:intergenic 25.0%
!!! AGATCTTTTCAAGATAGATT+CGG + chr2.2:54671437-54671456 MS.gene070232:CDS 25.0%
!!! ATTCTAGAGTCAATGTATTT+TGG + chr2.2:54671702-54671721 MS.gene070232:CDS 25.0%
!!! TGTTTTGAGAATTGTATGAA+AGG + chr2.2:54671660-54671679 MS.gene070232:CDS 25.0%
AATGGTCATTGTCTTCTTAT+TGG + chr2.2:54671915-54671934 MS.gene070232:CDS 30.0%
ACGAGAAAGAAAAACCAAAA+GGG - chr2.2:54670792-54670811 None:intergenic 30.0%
ATCCAACAATATGCAAAAGA+TGG + chr2.2:54671029-54671048 MS.gene070232:CDS 30.0%
ATTGATCTCGAAATTCGAAA+AGG + chr2.2:54671201-54671220 MS.gene070232:CDS 30.0%
CAAAATCATTAACAGGATGA+AGG - chr2.2:54670642-54670661 None:intergenic 30.0%
CAAACAGAAACAGAGTTAAT+TGG - chr2.2:54671954-54671973 None:intergenic 30.0%
GATATGATTCTTGTTGATGT+TGG - chr2.2:54670426-54670445 None:intergenic 30.0%
GGAATGTAAAGATTGATGTT+TGG + chr2.2:54671775-54671794 MS.gene070232:CDS 30.0%
GTTCAGTTTCAATATAGTTG+AGG + chr2.2:54670273-54670292 MS.gene070232:CDS 30.0%
TCATGGAATTAGGAATATTG+TGG + chr2.2:54671725-54671744 MS.gene070232:CDS 30.0%
TCCACTTTATGACACAAAAA+AGG + chr2.2:54670519-54670538 MS.gene070232:CDS 30.0%
TTGATCTCGAAATTCGAAAA+GGG + chr2.2:54671202-54671221 MS.gene070232:CDS 30.0%
TTTGTATCAAGCTGAATTAG+TGG + chr2.2:54671851-54671870 MS.gene070232:CDS 30.0%
! ATCTTTATTTGATCCTGAAG+AGG + chr2.2:54671053-54671072 MS.gene070232:CDS 30.0%
! CTTCATCCTGTTAATGATTT+TGG + chr2.2:54670640-54670659 MS.gene070232:CDS 30.0%
! TTCATCCGATGATACATTTT+GGG + chr2.2:54670306-54670325 MS.gene070232:CDS 30.0%
! TTCCATCTTTTGCATATTGT+TGG - chr2.2:54671034-54671053 None:intergenic 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.2:54670775-54670794 MS.gene070232:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.2:54671620-54671639 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.2:54671798-54671817 MS.gene070232:CDS 30.0%
!! ATTGTGATTAGCTTCAATCT+CGG - chr2.2:54671580-54671599 None:intergenic 30.0%
!! CAATTAACTCTGTTTCTGTT+TGG + chr2.2:54671952-54671971 MS.gene070232:CDS 30.0%
!!! TCCTTTTTTGTGTCATAAAG+TGG - chr2.2:54670523-54670542 None:intergenic 30.0%
!!! TGTATTTTGGTCATGGAATT+AGG + chr2.2:54671715-54671734 MS.gene070232:CDS 30.0%
AAGATAACAGCTATTGAGAG+CGG + chr2.2:54671288-54671307 MS.gene070232:CDS 35.0%
AAGGAGATTGGTAGAATGAT+TGG - chr2.2:54670546-54670565 None:intergenic 35.0%
ATTATGGCAAAGGATTCAAG+AGG + chr2.2:54670590-54670609 MS.gene070232:CDS 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.2:54670991-54671010 MS.gene070232:CDS 35.0%
CACGAGAAAGAAAAACCAAA+AGG - chr2.2:54670793-54670812 None:intergenic 35.0%
CATACAATCATCATCTTCGT+CGG + chr2.2:54670738-54670757 MS.gene070232:CDS 35.0%
GAAACGGTTCACAAAAGATT+TGG - chr2.2:54671604-54671623 None:intergenic 35.0%
GCTTGCACATTTGATATGAA+TGG + chr2.2:54671897-54671916 MS.gene070232:CDS 35.0%
GGTTTCCCAAAATGTATCAT+CGG - chr2.2:54670314-54670333 None:intergenic 35.0%
GTCATTGTCTTCTTATTGGA+TGG + chr2.2:54671919-54671938 MS.gene070232:CDS 35.0%
TGATAACCTACACTTTGACA+TGG + chr2.2:54670462-54670481 MS.gene070232:CDS 35.0%
TGTCTTCTTATTGGATGGAA+AGG + chr2.2:54671924-54671943 MS.gene070232:CDS 35.0%
! AATATGTCAACTGGTGCTAA+AGG - chr2.2:54670565-54670584 None:intergenic 35.0%
! AGGTTATTACCTGATGATGA+AGG + chr2.2:54670610-54670629 MS.gene070232:CDS 35.0%
! CACATGTGTACAAGCTTTAT+TGG + chr2.2:54671146-54671165 MS.gene070232:CDS 35.0%
! GTTCATCCGATGATACATTT+TGG + chr2.2:54670305-54670324 MS.gene070232:CDS 35.0%
! TTTCACAAGATTTGGAATGG+TGG + chr2.2:54671806-54671825 MS.gene070232:CDS 35.0%
!! AAATTCGAAAAGGGTGTCAA+TGG + chr2.2:54671211-54671230 MS.gene070232:CDS 35.0%
!! ATGATTGTTTCGAGTTTGTC+TGG - chr2.2:54671516-54671535 None:intergenic 35.0%
!! CTGTTTCTTTGTCGATTCTT+TGG - chr2.2:54670993-54671012 None:intergenic 35.0%
!! GATGTTGGTGATGATACTTT+AGG - chr2.2:54670411-54670430 None:intergenic 35.0%
!!! GAGTCAATGTATTTTGGTCA+TGG + chr2.2:54671708-54671727 MS.gene070232:CDS 35.0%
AAGCATATAGTTGAGGACAC+TGG + chr2.2:54671327-54671346 MS.gene070232:CDS 40.0%
AAGGAGCTGAAAGAAAGAGT+AGG + chr2.2:54671754-54671773 MS.gene070232:CDS 40.0%
AGGAATATTGTGGCTGAAGA+AGG + chr2.2:54671735-54671754 MS.gene070232:CDS 40.0%
AGGATTTCTCCTTCATCATC+AGG - chr2.2:54670622-54670641 None:intergenic 40.0%
AGTGAACCATGTCAAAGTGT+AGG - chr2.2:54670471-54670490 None:intergenic 40.0%
ATAGAGATCATGAAGCTAGC+TGG + chr2.2:54670706-54670725 MS.gene070232:CDS 40.0%
CACTTCGAAGCATATAGTTG+AGG + chr2.2:54671320-54671339 MS.gene070232:CDS 40.0%
GAAGATCTAGGAGACTATCA+TGG + chr2.2:54670376-54670395 MS.gene070232:CDS 40.0%
GAGTCTTTATCTTCGAAGAC+AGG + chr2.2:54670922-54670941 MS.gene070232:CDS 40.0%
GGACACTGGTAAAAGATTGA+AGG + chr2.2:54671341-54671360 MS.gene070232:CDS 40.0%
TAATGAACCACACGCTTAAC+CGG - chr2.2:54670952-54670971 None:intergenic 40.0%
! GTGGCTAAAAGATTTGCTTG+TGG + chr2.2:54671870-54671889 MS.gene070232:CDS 40.0%
! GTTATCTTTAGAAGCTCGAG+TGG - chr2.2:54671276-54671295 None:intergenic 40.0%
! TCGATTGAAGTGCTTGCATT+AGG - chr2.2:54671242-54671261 None:intergenic 40.0%
! TTTTCTGCAGCAGAAGATCT+AGG + chr2.2:54670364-54670383 MS.gene070232:CDS 40.0%
!! TTAGCTTGTGCTGAGAAAGT+CGG + chr2.2:54670856-54670875 MS.gene070232:CDS 40.0%
AATGAACCACACGCTTAACC+GGG - chr2.2:54670951-54670970 None:intergenic 45.0%
AGATCCTTGCTAACCTCTTC+AGG - chr2.2:54671069-54671088 None:intergenic 45.0%
AGATTCGGAAGAAACAGTCG+CGG + chr2.2:54671452-54671471 MS.gene070232:CDS 45.0%
ATCTTCGAAGACAGGTAACC+CGG + chr2.2:54670930-54670949 MS.gene070232:CDS 45.0%
GGTTAGCAAGGATCTAAACC+CGG + chr2.2:54671074-54671093 MS.gene070232:CDS 45.0%
TCATGAAGCTAGCTGGAACA+AGG + chr2.2:54670713-54670732 MS.gene070232:CDS 45.0%
TCGGCGACAACCATTACTAT+AGG - chr2.2:54671561-54671580 None:intergenic 45.0%
TGATCCTGAAGAGGTTAGCA+AGG + chr2.2:54671062-54671081 MS.gene070232:CDS 45.0%
! CAACTGGTGCTAAAGGAGAT+TGG - chr2.2:54670558-54670577 None:intergenic 45.0%
ATCTTCGTACAGCGCGATCA+TGG - chr2.2:54671100-54671119 None:intergenic 50.0%
TCTTCGTCGGAATCTGCATC+AGG + chr2.2:54670751-54670770 MS.gene070232:CDS 50.0%
AGGTAACCCGGTTAAGCGTG+TGG + chr2.2:54670942-54670961 MS.gene070232:CDS 55.0%
TCGTACAGCGCGATCATGGC+CGG - chr2.2:54671096-54671115 None:intergenic 60.0%
CGTACAGCGCGATCATGGCC+GGG - chr2.2:54671095-54671114 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 54670247 54671986 54670247 ID=MS.gene070232
chr2.2 mRNA 54670247 54671986 54670247 ID=MS.gene070232.t1;Parent=MS.gene070232
chr2.2 exon 54670247 54671986 54670247 ID=MS.gene070232.t1.exon1;Parent=MS.gene070232.t1
chr2.2 CDS 54670247 54671986 54670247 ID=cds.MS.gene070232.t1;Parent=MS.gene070232.t1
Gene Sequence

>MS.gene070232

ATGGAAAACTTGTGCAATTTTGATGAGTTCAGTTTCAATATAGTTGAGGATAAACTTAGTTCATCCGATGATACATTTTGGGAAACCAAGAAATTTAGTGAAATGAAAAATGTTAAATTTTCTGCAGCAGAAGATCTAGGAGACTATCATGGAATTGAGTTCCTAAAGTATCATCACCAACATCAACAAGAATCATATCAAGATTATGAAACTTTTGATAACCTACACTTTGACATGGTTCACTTTGATGAACAATGTCCTAAAAGAATTCTTCCACTTTATGACACAAAAAAGGACCAATCATTCTACCAATCTCCTTTAGCACCAGTTGACATATTGAAGAATTATGGCAAAGGATTCAAGAGGTTATTACCTGATGATGAAGGAGAAATCCTTCATCCTGTTAATGATTTTGGTTTAGTCACAAATAATGAAAATGAGAAAAAGTTGTTGTCAACTATAGAGATCATGAAGCTAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGGAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTCTTTCTCGTGTTTATCTGATGAAGAGAAAGATGTTTCGCTTGCTGAATCACTCTTAGCTTGTGCTGAGAAAGTCGGATATCAACAATATGAACGCGCTAGAAAATTGTTGTCACAAATCGAGTCTTTATCTTCGAAGACAGGTAACCCGGTTAAGCGTGTGGTTCATTATTTTGCTGAAGCTCTTCGCCAAAGAATCGACAAAGAAACAGGACGATTTTCTGTTTCATCCAACAATATGCAAAAGATGGAATCTTTATTTGATCCTGAAGAGGTTAGCAAGGATCTAAACCCGGCCATGATCGCGCTGTACGAAGATCTTCCATTCAGTCAAGTTTCAATATTCACATGTGTACAAGCTTTATTGGAAAATGTAAATGATGCAAAGAAGATTCATGTTATTGATCTCGAAATTCGAAAAGGGTGTCAATGGACAATCCTAATGCAAGCACTTCAATCGAGAAACGAATGTCCACTCGAGCTTCTAAAGATAACAGCTATTGAGAGCGGAAACTCAGACACTTCGAAGCATATAGTTGAGGACACTGGTAAAAGATTGAAGGATTTTGCGCAAAGTTTGAACATACCTTTTTCGTTCGATATAGTTATTGTATCTGATTTGTTACATATAAGAGAAGATCTTTTCAAGATAGATTCGGAAGAAACAGTCGCGGTTTATTCTCAATTTGCGCTTAGAAGCAAAATTCAACAACCAGACAAACTCGAAACAATCATGAGAGTGATTAGAACCATAAATCCTATAGTAATGGTTGTCGCCGAGATTGAAGCTAATCACAATTCCAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCATTTTTTGATTGTTTTGAGAATTGTATGAAAGGTGATGAGAAAAACAGATTTATTCTAGAGTCAATGTATTTTGGTCATGGAATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGGAATGTAAAGATTGATGTTTGGAGAGCATTTTTCACAAGATTTGGAATGGTGGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTAGTGGCTAAAAGATTTGCTTGTGGATATGCTTGCACATTTGATATGAATGGTCATTGTCTTCTTATTGGATGGAAAGGAACACCAATTAACTCTGTTTCTGTTTGGAAATTCATTTGA
Protein sequence

>MS.gene070232.t1

MENLCNFDEFSFNIVEDKLSSSDDTFWETKKFSEMKNVKFSAAEDLGDYHGIEFLKYHHQHQQESYQDYETFDNLHFDMVHFDEQCPKRILPLYDTKKDQSFYQSPLAPVDILKNYGKGFKRLLPDDEGEILHPVNDFGLVTNNENEKKLLSTIEIMKLAGTRFIQSSSSSESASGLILNHPFGFSFSCLSDEEKDVSLAESLLACAEKVGYQQYERARKLLSQIESLSSKTGNPVKRVVHYFAEALRQRIDKETGRFSVSSNNMQKMESLFDPEEVSKDLNPAMIALYEDLPFSQVSIFTCVQALLENVNDAKKIHVIDLEIRKGCQWTILMQALQSRNECPLELLKITAIESGNSDTSKHIVEDTGKRLKDFAQSLNIPFSFDIVIVSDLLHIREDLFKIDSEETVAVYSQFALRSKIQQPDKLETIMRVIRTINPIVMVVAEIEANHNSKSFVNRFIEALFYFSAFFDCFENCMKGDEKNRFILESMYFGHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFACGYACTFDMNGHCLLIGWKGTPINSVSVWKFI