AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058234

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058234.t1 MTR_2g034140 82.749 342 22 2 3 307 84 425 0.0 520
MS.gene058234.t1 MTR_2g034140 82.749 342 22 2 3 307 70 411 0.0 518
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATCTCTTATTGGCTCTA+AGG 0.283609 2.4:+56561253 MS.gene058234:CDS
GATATTACCTTGCACTTGTA+TGG 0.294342 2.4:-56559761 None:intergenic
ACAGGCTTTCTTCAACTTGC+TGG 0.301920 2.4:+56560766 MS.gene058234:intron
TCATCCTGAGCATCTCTTAT+TGG 0.306483 2.4:+56561245 MS.gene058234:CDS
CTTTAGTACTCTTGGTGTTT+CGG 0.308560 2.4:-56559392 None:intergenic
ACATCTTATGCTGCCTTAGA+AGG 0.333220 2.4:+56559071 MS.gene058234:CDS
GAAGAACTATGGCGTGCTTT+AGG 0.333345 2.4:+56559960 MS.gene058234:CDS
TATGCTGCCTTAGAAGGCCT+TGG 0.350897 2.4:+56559077 MS.gene058234:CDS
CGTCCGATGAGTGGAGATAT+TGG 0.364584 2.4:+56560339 MS.gene058234:CDS
TCATAACCCAAGGCCTTCTA+AGG 0.373122 2.4:-56559084 None:intergenic
AGCATAGCTTATGGCGGCTA+CGG 0.378096 2.4:+56561015 MS.gene058234:CDS
TCTAAGGGAAGCACTTGCTT+TGG 0.385951 2.4:+56561269 MS.gene058234:CDS
ATAGATTATAGACATTTAAC+AGG 0.392814 2.4:-56561297 None:intergenic
GTTAAAACCTTTAGTACTCT+TGG 0.395381 2.4:-56559400 None:intergenic
TTTGTGAAGTAGATTTCTCT+TGG 0.401255 2.4:-56559357 None:intergenic
TTAATTAGCAGCATAGCTTA+TGG 0.403083 2.4:+56561006 MS.gene058234:CDS
ATGCTGCCTTAGAAGGCCTT+GGG 0.406090 2.4:+56559078 MS.gene058234:CDS
ACGGGCTTTGCGATCATGAT+TGG 0.408171 2.4:-56559113 None:intergenic
GTAGACTGTTTGAAGAACTA+TGG 0.410606 2.4:+56559949 MS.gene058234:CDS
TTGTCTGTTTCCAGAATACT+TGG 0.416142 2.4:+56560939 MS.gene058234:intron
GTCCGATGAGTGGAGATATT+GGG 0.417237 2.4:+56560340 MS.gene058234:CDS
TGAAGCAGCTCCACCATGTT+GGG 0.428299 2.4:-56561214 None:intergenic
TGGAATGAGATTGCATTAGC+AGG 0.444449 2.4:-56559337 None:intergenic
ACTAATGCTCAGACAATTGA+AGG 0.476172 2.4:+56560793 MS.gene058234:CDS
AGGGAAGCACTTGCTTTGGT+TGG 0.480501 2.4:+56561273 MS.gene058234:CDS
AGAGCCAATAAGAGATGCTC+AGG 0.491628 2.4:-56561249 None:intergenic
TATTGGGAGAGGAGCAACTA+AGG 0.495509 2.4:+56560356 MS.gene058234:CDS
CGAAACACCAAGAGTACTAA+AGG 0.504880 2.4:+56559393 MS.gene058234:CDS
CAGTCCGATGGACAGTCCTC+AGG 0.509146 2.4:-56559289 None:intergenic
GCATAGCTTATGGCGGCTAC+GGG 0.510440 2.4:+56561016 MS.gene058234:CDS
AATAGCCTTTGCTGCTCCGT+TGG 0.515514 2.4:+56560383 MS.gene058234:CDS
TGCTAATTAACGCACATGAC+GGG 0.517376 2.4:-56560993 None:intergenic
GGCTTTCTTCAACTTGCTGG+TGG 0.517586 2.4:+56560769 MS.gene058234:intron
CATGCAGGACGGAGTTATAA+CGG 0.522415 2.4:+56559516 MS.gene058234:intron
TGGCATTCTGTTCAGAATGT+TGG 0.525755 2.4:+56561168 MS.gene058234:intron
GTTATAACGGAGCGCTGCTA+TGG 0.543440 2.4:+56559529 MS.gene058234:CDS
AAAGCAAAAGATAGACCTCC+TGG 0.545248 2.4:+56560408 MS.gene058234:CDS
CTTAACAGAATTTGATCCTG+AGG 0.554620 2.4:+56559273 MS.gene058234:intron
GCATCTCTTATTGGCTCTAA+GGG 0.555107 2.4:+56561254 MS.gene058234:CDS
TTGAAGCAGCTCCACCATGT+TGG 0.555464 2.4:-56561215 None:intergenic
TTTAGCCAACGGAGCAGCAA+AGG 0.562360 2.4:-56560388 None:intergenic
TCCACCATGTTGGGATTGCA+TGG 0.562511 2.4:-56561205 None:intergenic
CTGAGGACTGTCCATCGGAC+TGG 0.565863 2.4:+56559290 MS.gene058234:CDS
CTGCTAATTAACGCACATGA+CGG 0.568256 2.4:-56560994 None:intergenic
TGATCCTGAGGACTGTCCAT+CGG 0.576334 2.4:+56559285 MS.gene058234:CDS
ACGGAGCGCTGCTATGGCTG+TGG 0.581963 2.4:+56559535 MS.gene058234:CDS
GTTGTGAGTGCTGTGAATGA+AGG 0.582088 2.4:+56559031 MS.gene058234:CDS
GGAATGAGATTGCATTAGCA+GGG 0.583303 2.4:-56559336 None:intergenic
ATGAGTGGAGATATTGGGAG+AGG 0.585956 2.4:+56560345 MS.gene058234:CDS
TTAGACGGCCGTCCGATGAG+TGG 0.595300 2.4:+56560330 MS.gene058234:CDS
CAAGGGCGTGACCAGTCCGA+TGG 0.597365 2.4:-56559301 None:intergenic
TTATGGCGGCTACGGGCGAA+AGG 0.598825 2.4:+56561023 MS.gene058234:CDS
TTAAAATCCATACAAGTGCA+AGG 0.612983 2.4:+56559754 MS.gene058234:CDS
CTCCCAATATCTCCACTCAT+CGG 0.614980 2.4:-56560342 None:intergenic
TTGTATAAACTCAAGTTAGA+CGG 0.625221 2.4:+56560315 MS.gene058234:intron
TGGTGGAGCTGCTTCAATCG+AGG 0.649967 2.4:+56561221 MS.gene058234:CDS
ATTAGCAGCATAGCTTATGG+CGG 0.658854 2.4:+56561009 MS.gene058234:CDS
AGATCCATGCAATCCCAACA+TGG 0.661159 2.4:+56561201 MS.gene058234:CDS
CAATATCTCCACTCATCGGA+CGG 0.667049 2.4:-56560338 None:intergenic
TTGGCACTGATCATAACCCA+AGG 0.695152 2.4:-56559094 None:intergenic
TCCATGCAATCCCAACATGG+TGG 0.701127 2.4:+56561204 MS.gene058234:CDS
TGTGAATGAAGGAATTGAAG+TGG 0.703438 2.4:+56559042 MS.gene058234:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACCTCTTCTAATATTTAAT+GGG + chr2.4:56560029-56560048 MS.gene058234:intron 20.0%
!! TAAAACTTGTGATGTTTAAT+TGG + chr2.4:56559487-56559506 MS.gene058234:intron 20.0%
!!! TCAATTTGTTAAAAGAAACA+TGG - chr2.4:56560287-56560306 None:intergenic 20.0%
! AACCCATTAAATATTAGAAG+AGG - chr2.4:56560034-56560053 None:intergenic 25.0%
! AAGAAACATGGAATAGAATT+TGG - chr2.4:56560275-56560294 None:intergenic 25.0%
! ACGTTACACAAAACTAATTA+AGG - chr2.4:56559642-56559661 None:intergenic 25.0%
! ATTGAAGGAATGAAAAAAGA+AGG + chr2.4:56560808-56560827 MS.gene058234:CDS 25.0%
! ATTTACTATTGAAATGATCC+AGG + chr2.4:56560110-56560129 MS.gene058234:intron 25.0%
! CAACCTCTTCTAATATTTAA+TGG + chr2.4:56560028-56560047 MS.gene058234:intron 25.0%
! CGTTACACAAAACTAATTAA+GGG - chr2.4:56559641-56559660 None:intergenic 25.0%
! GACATGATATGAATCTAAAA+TGG + chr2.4:56560642-56560661 MS.gene058234:intron 25.0%
! TAAATATTAGAAGAGGTTGA+AGG - chr2.4:56560027-56560046 None:intergenic 25.0%
! TTCCATAAATACTTGTTTCA+AGG + chr2.4:56560066-56560085 MS.gene058234:intron 25.0%
! TTGTATAAACTCAAGTTAGA+CGG + chr2.4:56560315-56560334 MS.gene058234:intron 25.0%
!!! ATCGTACTTATTTTATCGTA+GGG - chr2.4:56559580-56559599 None:intergenic 25.0%
!!! GGAAAAGATTTTAATGAGAT+AGG + chr2.4:56560555-56560574 MS.gene058234:intron 25.0%
!!! TATCGTACTTATTTTATCGT+AGG - chr2.4:56559581-56559600 None:intergenic 25.0%
AGCCTTGAAACAAGTATTTA+TGG - chr2.4:56560071-56560090 None:intergenic 30.0%
AGGATCAAATTCTGTTAAGA+AGG - chr2.4:56559272-56559291 None:intergenic 30.0%
ATTGTTGAATCTCCAACATT+AGG - chr2.4:56560144-56560163 None:intergenic 30.0%
CAGTTATATCCTAGTATTTC+AGG - chr2.4:56559793-56559812 None:intergenic 30.0%
CTCAAGTTATCATATCATGA+TGG + chr2.4:56560599-56560618 MS.gene058234:intron 30.0%
GAAACCAAATCTTAGAAACT+TGG + chr2.4:56560194-56560213 MS.gene058234:intron 30.0%
GAAGTTTAATCTATGCTTAC+CGG + chr2.4:56559221-56559240 MS.gene058234:intron 30.0%
GTCTAACTTGAGTTTATACA+AGG - chr2.4:56560316-56560335 None:intergenic 30.0%
GTTAAAACCTTTAGTACTCT+TGG - chr2.4:56559403-56559422 None:intergenic 30.0%
GTTCAATAAGAACTGTCTTA+CGG - chr2.4:56559135-56559154 None:intergenic 30.0%
TAACAACATTGAGTGATTAC+CGG - chr2.4:56559243-56559262 None:intergenic 30.0%
TTAAAATCCATACAAGTGCA+AGG + chr2.4:56559754-56559773 MS.gene058234:CDS 30.0%
TTAATTAGCAGCATAGCTTA+TGG + chr2.4:56561006-56561025 MS.gene058234:CDS 30.0%
TTCAATAAGAACTGTCTTAC+GGG - chr2.4:56559134-56559153 None:intergenic 30.0%
TTTAATTGGATTGAACATGC+AGG + chr2.4:56559501-56559520 MS.gene058234:intron 30.0%
TTTGTGAAGTAGATTTCTCT+TGG - chr2.4:56559360-56559379 None:intergenic 30.0%
! ATCAGTTTTCCTGAAATACT+AGG + chr2.4:56559781-56559800 MS.gene058234:intron 30.0%
! TTTTCATGATCCAAGTATTC+TGG - chr2.4:56560952-56560971 None:intergenic 30.0%
!! AAGATTTTAATGAGATAGGC+AGG + chr2.4:56560559-56560578 MS.gene058234:intron 30.0%
!! TGTACTTTTCATTTTGATCC+AGG + chr2.4:56559663-56559682 MS.gene058234:intron 30.0%
!!! TCGTACTTATTTTATCGTAG+GGG - chr2.4:56559579-56559598 None:intergenic 30.0%
AAGAAAGCCTGTCATGTTAA+GGG - chr2.4:56560758-56560777 None:intergenic 35.0%
ACTAATGCTCAGACAATTGA+AGG + chr2.4:56560793-56560812 MS.gene058234:CDS 35.0%
ATGATACGTATAAACCCTGA+AGG - chr2.4:56560715-56560734 None:intergenic 35.0%
CAACATTAGGTAAACTAACC+TGG - chr2.4:56560131-56560150 None:intergenic 35.0%
CTCACATTATGTGATTCCAT+TGG + chr2.4:56561148-56561167 MS.gene058234:intron 35.0%
CTTAACAGAATTTGATCCTG+AGG + chr2.4:56559273-56559292 MS.gene058234:intron 35.0%
GATAGGATATGGACATGATA+AGG + chr2.4:56560504-56560523 MS.gene058234:intron 35.0%
GATATTACCTTGCACTTGTA+TGG - chr2.4:56559764-56559783 None:intergenic 35.0%
GTAGACTGTTTGAAGAACTA+TGG + chr2.4:56559949-56559968 MS.gene058234:CDS 35.0%
GTATAAGTAATGCTATCACG+TGG + chr2.4:56560848-56560867 MS.gene058234:intron 35.0%
TGCAAGTGAGAATAGAAAAG+AGG - chr2.4:56560907-56560926 None:intergenic 35.0%
TGTGAATGAAGGAATTGAAG+TGG + chr2.4:56559042-56559061 MS.gene058234:CDS 35.0%
TTGTCTGTTTCCAGAATACT+TGG + chr2.4:56560939-56560958 MS.gene058234:intron 35.0%
TTTCTTTCCCTTAACATGAC+AGG + chr2.4:56560748-56560767 MS.gene058234:intron 35.0%
! ATAAGGTCTTATCCTCATGT+TGG + chr2.4:56560521-56560540 MS.gene058234:intron 35.0%
! CTATCTTTTGCTTTAGCCAA+CGG - chr2.4:56560402-56560421 None:intergenic 35.0%
! TTTGATGGTCATGATAGAGA+CGG + chr2.4:56560482-56560501 MS.gene058234:intron 35.0%
!! CAGGTTAGTTTACCTAATGT+TGG + chr2.4:56560129-56560148 MS.gene058234:intron 35.0%
!! CTTTAGTACTCTTGGTGTTT+CGG - chr2.4:56559395-56559414 None:intergenic 35.0%
!! TTTTCATTTTGATCCAGGAG+AGG + chr2.4:56559668-56559687 MS.gene058234:intron 35.0%
AAAGCAAAAGATAGACCTCC+TGG + chr2.4:56560408-56560427 MS.gene058234:CDS 40.0%
AAATGTGAATACGAACCAGG+AGG - chr2.4:56560426-56560445 None:intergenic 40.0%
ACATCTTATGCTGCCTTAGA+AGG + chr2.4:56559071-56559090 MS.gene058234:CDS 40.0%
ATTAGCAGCATAGCTTATGG+CGG + chr2.4:56561009-56561028 MS.gene058234:CDS 40.0%
ATTGGATTGAACATGCAGGA+CGG + chr2.4:56559505-56559524 MS.gene058234:intron 40.0%
CAGGATAAGAGAGACATGAT+AGG - chr2.4:56560679-56560698 None:intergenic 40.0%
CATTCTGAACAGAATGCCAA+TGG - chr2.4:56561167-56561186 None:intergenic 40.0%
CCAAATCTTAGAAACTTGGC+AGG + chr2.4:56560198-56560217 MS.gene058234:intron 40.0%
CTCTATCATGACCATCAAAC+AGG - chr2.4:56560481-56560500 None:intergenic 40.0%
CTGCTAATTAACGCACATGA+CGG - chr2.4:56560997-56561016 None:intergenic 40.0%
CTTACATATGTTACCTCTCC+TGG - chr2.4:56559684-56559703 None:intergenic 40.0%
GAAGAAAGCCTGTCATGTTA+AGG - chr2.4:56560759-56560778 None:intergenic 40.0%
GGAATGAGATTGCATTAGCA+GGG - chr2.4:56559339-56559358 None:intergenic 40.0%
TCATCCTGAGCATCTCTTAT+TGG + chr2.4:56561245-56561264 MS.gene058234:CDS 40.0%
TGATAGAGACGGATAGGATA+TGG + chr2.4:56560493-56560512 MS.gene058234:intron 40.0%
TGCTAATTAACGCACATGAC+GGG - chr2.4:56560996-56561015 None:intergenic 40.0%
TGGAAATGTGAATACGAACC+AGG - chr2.4:56560429-56560448 None:intergenic 40.0%
TGGAATGAGATTGCATTAGC+AGG - chr2.4:56559340-56559359 None:intergenic 40.0%
TGGCATTCTGTTCAGAATGT+TGG + chr2.4:56561168-56561187 MS.gene058234:intron 40.0%
! ATTTTATCGTAGGGGCAAAC+TGG - chr2.4:56559571-56559590 None:intergenic 40.0%
! CAGGGCAAACATTTTCACAA+GGG - chr2.4:56559321-56559340 None:intergenic 40.0%
! GATGTATCGACACAAGGAAA+TGG - chr2.4:56559915-56559934 None:intergenic 40.0%
! GGTTTTCAAAGGACTGTTGA+TGG - chr2.4:56560449-56560468 None:intergenic 40.0%
! GTGTCGATACATCCTTCTAT+AGG + chr2.4:56559922-56559941 MS.gene058234:intron 40.0%
! TAGAAGGATGTATCGACACA+AGG - chr2.4:56559921-56559940 None:intergenic 40.0%
!! AGCATCTCTTATTGGCTCTA+AGG + chr2.4:56561253-56561272 MS.gene058234:CDS 40.0%
!! CCTCATGTTGGTTTCTTACT+CGG + chr2.4:56560533-56560552 MS.gene058234:intron 40.0%
!! CCTGCCAAGTTTCTAAGATT+TGG - chr2.4:56560201-56560220 None:intergenic 40.0%
!! CGAAACACCAAGAGTACTAA+AGG + chr2.4:56559393-56559412 MS.gene058234:CDS 40.0%
!! CTCATGTTGGTTTCTTACTC+GGG + chr2.4:56560534-56560553 MS.gene058234:intron 40.0%
!! GCATCTCTTATTGGCTCTAA+GGG + chr2.4:56561254-56561273 MS.gene058234:CDS 40.0%
!! GGTCTTGTGAAGGTTTTCAA+AGG - chr2.4:56560460-56560479 None:intergenic 40.0%
ACAGGCTTTCTTCAACTTGC+TGG + chr2.4:56560766-56560785 MS.gene058234:intron 45.0%
AGAGCCAATAAGAGATGCTC+AGG - chr2.4:56561252-56561271 None:intergenic 45.0%
AGATCCATGCAATCCCAACA+TGG + chr2.4:56561201-56561220 MS.gene058234:CDS 45.0%
ATGAGTGGAGATATTGGGAG+AGG + chr2.4:56560345-56560364 MS.gene058234:CDS 45.0%
CAATATCTCCACTCATCGGA+CGG - chr2.4:56560341-56560360 None:intergenic 45.0%
CAGTCTACTCTGCCTATAGA+AGG - chr2.4:56559937-56559956 None:intergenic 45.0%
CATGCAGGACGGAGTTATAA+CGG + chr2.4:56559516-56559535 MS.gene058234:intron 45.0%
CCGAGTAAGAAACCAACATG+AGG - chr2.4:56560536-56560555 None:intergenic 45.0%
CCTTCACAAGACCTGTTTGA+TGG + chr2.4:56560467-56560486 MS.gene058234:intron 45.0%
CTCCCAATATCTCCACTCAT+CGG - chr2.4:56560345-56560364 None:intergenic 45.0%
GAAGAACTATGGCGTGCTTT+AGG + chr2.4:56559960-56559979 MS.gene058234:CDS 45.0%
GTATGCTGACGTGTGATGTA+TGG - chr2.4:56559167-56559186 None:intergenic 45.0%
GTCCGATGAGTGGAGATATT+GGG + chr2.4:56560340-56560359 MS.gene058234:CDS 45.0%
TATTGGGAGAGGAGCAACTA+AGG + chr2.4:56560356-56560375 MS.gene058234:CDS 45.0%
TCATAACCCAAGGCCTTCTA+AGG - chr2.4:56559087-56559106 None:intergenic 45.0%
TCTTAGAAACTTGGCAGGTG+TGG + chr2.4:56560203-56560222 MS.gene058234:intron 45.0%
TCTTATCCTGACCATCGAAC+AGG + chr2.4:56560689-56560708 MS.gene058234:intron 45.0%
TGGTCATGATAGAGACGGAT+AGG + chr2.4:56560487-56560506 MS.gene058234:intron 45.0%
! CCATCAAACAGGTCTTGTGA+AGG - chr2.4:56560470-56560489 None:intergenic 45.0%
! GCAGGGCAAACATTTTCACA+AGG - chr2.4:56559322-56559341 None:intergenic 45.0%
! GTTGTGAGTGCTGTGAATGA+AGG + chr2.4:56559031-56559050 MS.gene058234:CDS 45.0%
!! TCTAAGGGAAGCACTTGCTT+TGG + chr2.4:56561269-56561288 MS.gene058234:CDS 45.0%
!! TTGGCACTGATCATAACCCA+AGG - chr2.4:56559097-56559116 None:intergenic 45.0%
AATAGCCTTTGCTGCTCCGT+TGG + chr2.4:56560383-56560402 MS.gene058234:CDS 50.0%
ACGGGCTTTGCGATCATGAT+TGG - chr2.4:56559116-56559135 None:intergenic 50.0%
AGCATAGCTTATGGCGGCTA+CGG + chr2.4:56561015-56561034 MS.gene058234:CDS 50.0%
ATGCTGCCTTAGAAGGCCTT+GGG + chr2.4:56559078-56559097 MS.gene058234:CDS 50.0%
CGTCCGATGAGTGGAGATAT+TGG + chr2.4:56560339-56560358 MS.gene058234:CDS 50.0%
TATGCTGCCTTAGAAGGCCT+TGG + chr2.4:56559077-56559096 MS.gene058234:CDS 50.0%
TCCACCATGTTGGGATTGCA+TGG - chr2.4:56561208-56561227 None:intergenic 50.0%
TCCATGCAATCCCAACATGG+TGG + chr2.4:56561204-56561223 MS.gene058234:CDS 50.0%
TGAAGCAGCTCCACCATGTT+GGG - chr2.4:56561217-56561236 None:intergenic 50.0%
TGATCCTGAGGACTGTCCAT+CGG + chr2.4:56559285-56559304 MS.gene058234:CDS 50.0%
TTGAAGCAGCTCCACCATGT+TGG - chr2.4:56561218-56561237 None:intergenic 50.0%
TTGAAGCTAGTCGCCGTAAG+TGG + chr2.4:56559999-56560018 MS.gene058234:intron 50.0%
TTTAGCCAACGGAGCAGCAA+AGG - chr2.4:56560391-56560410 None:intergenic 50.0%
!! AGGGAAGCACTTGCTTTGGT+TGG + chr2.4:56561273-56561292 MS.gene058234:CDS 50.0%
!! GAGGTTGAAGGCACCACTTA+CGG - chr2.4:56560015-56560034 None:intergenic 50.0%
!! GGCTTTCTTCAACTTGCTGG+TGG + chr2.4:56560769-56560788 MS.gene058234:intron 50.0%
!! GTTATAACGGAGCGCTGCTA+TGG + chr2.4:56559529-56559548 MS.gene058234:CDS 50.0%
AACCCTGAAGGCCTGTTCGA+TGG - chr2.4:56560703-56560722 None:intergenic 55.0%
GACCATCGAACAGGCCTTCA+GGG + chr2.4:56560698-56560717 MS.gene058234:intron 55.0%
GCATAGCTTATGGCGGCTAC+GGG + chr2.4:56561016-56561035 MS.gene058234:CDS 55.0%
TGAAGGCCTGTTCGATGGTC+AGG - chr2.4:56560698-56560717 None:intergenic 55.0%
TGACCATCGAACAGGCCTTC+AGG + chr2.4:56560697-56560716 MS.gene058234:intron 55.0%
TGGTGGAGCTGCTTCAATCG+AGG + chr2.4:56561221-56561240 MS.gene058234:CDS 55.0%
CAGTCCGATGGACAGTCCTC+AGG - chr2.4:56559292-56559311 None:intergenic 60.0%
CTGAGGACTGTCCATCGGAC+TGG + chr2.4:56559290-56559309 MS.gene058234:CDS 60.0%
TTAGACGGCCGTCCGATGAG+TGG + chr2.4:56560330-56560349 MS.gene058234:CDS 60.0%
TTATGGCGGCTACGGGCGAA+AGG + chr2.4:56561023-56561042 MS.gene058234:CDS 60.0%
! CAAGGGCGTGACCAGTCCGA+TGG - chr2.4:56559304-56559323 None:intergenic 65.0%
!! ACGGAGCGCTGCTATGGCTG+TGG + chr2.4:56559535-56559554 MS.gene058234:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 56558983 56561314 56558983 ID=MS.gene058234
chr2.4 mRNA 56558983 56561314 56558983 ID=MS.gene058234.t1;Parent=MS.gene058234
chr2.4 exon 56558983 56559133 56558983 ID=MS.gene058234.t1.exon1;Parent=MS.gene058234.t1
chr2.4 CDS 56558983 56559133 56558983 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56559281 56559414 56559281 ID=MS.gene058234.t1.exon2;Parent=MS.gene058234.t1
chr2.4 CDS 56559281 56559414 56559281 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56559523 56559592 56559523 ID=MS.gene058234.t1.exon3;Parent=MS.gene058234.t1
chr2.4 CDS 56559523 56559592 56559523 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56559685 56559775 56559685 ID=MS.gene058234.t1.exon4;Parent=MS.gene058234.t1
chr2.4 CDS 56559685 56559775 56559685 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56559944 56560013 56559944 ID=MS.gene058234.t1.exon5;Parent=MS.gene058234.t1
chr2.4 CDS 56559944 56560013 56559944 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56560330 56560429 56560330 ID=MS.gene058234.t1.exon6;Parent=MS.gene058234.t1
chr2.4 CDS 56560330 56560429 56560330 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56560770 56560853 56560770 ID=MS.gene058234.t1.exon7;Parent=MS.gene058234.t1
chr2.4 CDS 56560770 56560853 56560770 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56560953 56561044 56560953 ID=MS.gene058234.t1.exon8;Parent=MS.gene058234.t1
chr2.4 CDS 56560953 56561044 56560953 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
chr2.4 exon 56561183 56561314 56561183 ID=MS.gene058234.t1.exon9;Parent=MS.gene058234.t1
chr2.4 CDS 56561183 56561314 56561183 ID=cds.MS.gene058234.t1;Parent=MS.gene058234.t1
Gene Sequence

>MS.gene058234

ATGTGGTTGATTGCAGTTGACTGCATTGACTGTGCTGCTGATGCATCTGTTGTGAGTGCTGTGAATGAAGGAATTGAAGTGGCAAGAGACATCTTATGCTGCCTTAGAAGGCCTTGGGTTATGATCAGTGCCAATCATGATCGCAAAGCCCGTAAGACAGTTCTTATTGAACTTAATTGTTCCATACATCACACGTCAGCATACATCAGAAGTTTATGTACAAAATTTAACAAAATATGAAGTTTAATCTATGCTTACCGGTAATCACTCAATGTTGTTAGTAAATCCTTCTTAACAGAATTTGATCCTGAGGACTGTCCATCGGACTGGTCACGCCCTTGTGAAAATGTTTGCCCTGCTAATGCAATCTCATTCCAAGAGAAATCTACTTCACAAATTTCATGTAACACCGAAACACCAAGAGTACTAAAGGTTTTAACTTGATTTTACTCAAAATCTCGAAGTTTGTGTTCTGATATACACAAAAACTGTCTTTTTAAAATATAAAACTTGTGATGTTTAATTGGATTGAACATGCAGGACGGAGTTATAACGGAGCGCTGCTATGGCTGTGGTCGCTGTCTTCCAGTTTGCCCCTACGATAAAATAAGTACGATATTTTCTAGTTTCATCTGATATTATAAATCTTAGCTTTCCCTTAATTAGTTTTGTGTAACGTATGTACTTTTCATTTTGATCCAGGAGAGGTAACATATGTAAGAGATGCTGTTACAACTTCTGATCTCATCAAGAGAAACGATGTTGATGCTATTAAAATCCATACAAGTGCAAGGTAATATCAGTTTTCCTGAAATACTAGGATATAACTGCAATTGCTATGAAAATACATTTCATGATTGCTATTCACAAAGTTTTATTAATCAAGTAATTAATGTTATTTCTATGATCTTTGATTGATTTTTCGAAATCCATTTCCTTGTGTCGATACATCCTTCTATAGGCAGAGTAGACTGTTTGAAGAACTATGGCGTGCTTTAGGAGATTCAGTAGAAAATTTGAAGCTAGTCGCCGTAAGTGGTGCCTTCAACCTCTTCTAATATTTAATGGGTTATAGTATTAGCTTTCCATAAATACTTGTTTCAAGGCTATTCTCATTTAATTCAACAATTTACTATTGAAATGATCCAGGTTAGTTTACCTAATGTTGGAGATTCAACAATATCTTCTATGAACAAAATGTTCTCTATTATGAAACCAAATCTTAGAAACTTGGCAGGTGTGGCATTTCTCTCTTTTCAACAGATTGATCAATTTTATATTCTTATAAGCCAAATTCTATTCCATGTTTCTTTTAACAAATTGATCTCTGCCTTGTATAAACTCAAGTTAGACGGCCGTCCGATGAGTGGAGATATTGGGAGAGGAGCAACTAAGGCATCAATAGCCTTTGCTGCTCCGTTGGCTAAAGCAAAAGATAGACCTCCTGGTTCGTATTCACATTTCCATCAACAGTCCTTTGAAAACCTTCACAAGACCTGTTTGATGGTCATGATAGAGACGGATAGGATATGGACATGATAAGGTCTTATCCTCATGTTGGTTTCTTACTCGGGAAAAGATTTTAATGAGATAGGCAGGATATGATATGCGACATGCTCAAGTTATCATATCATGATGGCTCGTTAAACACTCGATTGAGACATGATATGAATCTAAAATGGTTTAACTTTATCCTATCATGTCTCTCTTATCCTGACCATCGAACAGGCCTTCAGGGTTTATACGTATCATGATTGAACTGATCTTTCTTTCCCTTAACATGACAGGCTTTCTTCAACTTGCTGGTGGAACTAATGCTCAGACAATTGAAGGAATGAAAAAAGAAGGATTCTTTCAAACAACTAGTATAAGTAATGCTATCACGTGGTTACTTGATACTTTGTTTAGAATCTTGATACTTCCTCTTTTCTATTCTCACTTGCATTATAAGCATTCTTGTCTGTTTCCAGAATACTTGGATCATGAAAATTCAACAGTCAGTACATCTAACCCGTCATGTGCGTTAATTAGCAGCATAGCTTATGGCGGCTACGGGCGAAAGGTAACGCAACTATTTTTGTATGTCTGATTATTCATTTTTCATTCTGTTTCTGTTCTAAATTTTCTGTAGTTAGATAAAATAATAAATTTGAGTCACAACTAGACTCACATTATGTGATTCCATTGGCATTCTGTTCAGAATGTTGGTAGAATATTAAGATCCATGCAATCCCAACATGGTGGAGCTGCTTCAATCGAGGATCATCCTGAGCATCTCTTATTGGCTCTAAGGGAAGCACTTGCTTTGGTTGGACCTGTTAAATGTCTATAA
Protein sequence

>MS.gene058234.t1

MWLIAVDCIDCAADASVVSAVNEGIEVARDILCCLRRPWVMISANHDRKAQFDPEDCPSDWSRPCENVCPANAISFQEKSTSQISCNTETPRVLKDGVITERCYGCGRCLPVCPYDKIREVTYVRDAVTTSDLIKRNDVDAIKIHTSARQSRLFEELWRALGDSVENLKLVALDGRPMSGDIGRGATKASIAFAAPLAKAKDRPPGFLQLAGGTNAQTIEGMKKEGFFQTTSIKYLDHENSTVSTSNPSCALISSIAYGGYGRKNVGRILRSMQSQHGGAASIEDHPEHLLLALREALALVGPVKCL