Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058263.t1 | XP_003594702.1 | 96.4 | 112 | 4 | 0 | 1 | 112 | 67 | 178 | 1.90E-52 | 214.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058263.t1 | A2Q674 | 96.4 | 112 | 4 | 0 | 1 | 112 | 67 | 178 | 1.3e-52 | 214.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058263 | MS.gene55964 | 0.848827 | 4.78E-60 | -1.69E-46 |
| MS.gene058263 | MS.gene67711 | 0.894287 | 2.84E-75 | -1.69E-46 |
| MS.gene058263 | MS.gene67741 | 0.990489 | 7.07E-183 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058263.t1 | MTR_2g033630 | 96.429 | 112 | 4 | 0 | 1 | 112 | 67 | 178 | 6.98e-77 | 224 |
| MS.gene058263.t1 | MTR_4g122740 | 69.643 | 112 | 33 | 1 | 1 | 112 | 69 | 179 | 2.35e-52 | 162 |
| MS.gene058263.t1 | MTR_4g122720 | 55.357 | 112 | 50 | 0 | 1 | 112 | 76 | 187 | 4.12e-44 | 142 |
| MS.gene058263.t1 | MTR_4g122718 | 50.893 | 112 | 52 | 1 | 1 | 112 | 1 | 109 | 2.91e-40 | 129 |
| MS.gene058263.t1 | MTR_4g122712 | 30.357 | 112 | 75 | 1 | 1 | 109 | 62 | 173 | 5.70e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058263.t1 | AT3G17130 | 42.500 | 120 | 58 | 2 | 1 | 112 | 66 | 182 | 2.10e-26 | 97.1 |
Find 13 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTGTTATGTCACCTTTA+GGG | 0.220441 | 2.2:-55144656 | MS.gene058263:CDS |
| GGGGTTGTTATGTCACCTTT+AGG | 0.229357 | 2.2:-55144657 | MS.gene058263:CDS |
| GCTATAAATCAAAACCGGTT+TGG | 0.390286 | 2.2:-55144744 | MS.gene058263:CDS |
| TATTATTAAACAACTATTGA+AGG | 0.404982 | 2.2:-55144589 | MS.gene058263:CDS |
| GAAAACTACAGATCGTGAAA+TGG | 0.467104 | 2.2:-55144838 | MS.gene058263:CDS |
| GGAAGAGTGTACTTCACAAT+TGG | 0.475484 | 2.2:+55144780 | None:intergenic |
| GGTTGTTATGTCACCTTTAG+GGG | 0.492197 | 2.2:-55144655 | MS.gene058263:CDS |
| CTGATGCTATAAATCAAAAC+CGG | 0.505756 | 2.2:-55144749 | MS.gene058263:CDS |
| AATAACTAGCAAATCCAAAC+CGG | 0.527876 | 2.2:+55144730 | None:intergenic |
| ATACCATTTCTATCCCCTAA+AGG | 0.536471 | 2.2:+55144642 | None:intergenic |
| ATTATTAAACAACTATTGAA+GGG | 0.542633 | 2.2:-55144588 | MS.gene058263:CDS |
| TCACCTTTAGGGGATAGAAA+TGG | 0.544897 | 2.2:-55144645 | MS.gene058263:CDS |
| TTTATAGCATCAGCAGCTTG+TGG | 0.575094 | 2.2:+55144759 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTAAACAACTATTGAA+GGG | - | chr2.2:55144896-55144915 | MS.gene058263:CDS | 15.0% |
| !! | TATTATTAAACAACTATTGA+AGG | - | chr2.2:55144895-55144914 | MS.gene058263:CDS | 15.0% |
| !!! | CTTGTAATAAGAAATTTTCT+GGG | - | chr2.2:55144807-55144826 | MS.gene058263:CDS | 20.0% |
| !!! | TCTTGTAATAAGAAATTTTC+TGG | - | chr2.2:55144806-55144825 | MS.gene058263:CDS | 20.0% |
| !!! | TTGTAATAAGAAATTTTCTG+GGG | - | chr2.2:55144808-55144827 | MS.gene058263:CDS | 20.0% |
| AATAACTAGCAAATCCAAAC+CGG | + | chr2.2:55144757-55144776 | None:intergenic | 30.0% | |
| CTGATGCTATAAATCAAAAC+CGG | - | chr2.2:55144735-55144754 | MS.gene058263:CDS | 30.0% | |
| ATACCATTTCTATCCCCTAA+AGG | + | chr2.2:55144845-55144864 | None:intergenic | 35.0% | |
| GAAAACTACAGATCGTGAAA+TGG | - | chr2.2:55144646-55144665 | MS.gene058263:CDS | 35.0% | |
| GCTATAAATCAAAACCGGTT+TGG | - | chr2.2:55144740-55144759 | MS.gene058263:CDS | 35.0% | |
| ! | TCGTGAAATGGAAAAAGCAT+TGG | - | chr2.2:55144658-55144677 | MS.gene058263:CDS | 35.0% |
| GGGTTGTTATGTCACCTTTA+GGG | - | chr2.2:55144828-55144847 | MS.gene058263:CDS | 40.0% | |
| GGTTGTTATGTCACCTTTAG+GGG | - | chr2.2:55144829-55144848 | MS.gene058263:CDS | 40.0% | |
| TTTATAGCATCAGCAGCTTG+TGG | + | chr2.2:55144728-55144747 | None:intergenic | 40.0% | |
| ! | GGAAGAGTGTACTTCACAAT+TGG | + | chr2.2:55144707-55144726 | None:intergenic | 40.0% |
| ! | TCACCTTTAGGGGATAGAAA+TGG | - | chr2.2:55144839-55144858 | MS.gene058263:CDS | 40.0% |
| GGGGTTGTTATGTCACCTTT+AGG | - | chr2.2:55144827-55144846 | MS.gene058263:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 55144584 | 55144922 | 55144584 | ID=MS.gene058263 |
| chr2.2 | mRNA | 55144584 | 55144922 | 55144584 | ID=MS.gene058263.t1;Parent=MS.gene058263 |
| chr2.2 | exon | 55144584 | 55144922 | 55144584 | ID=MS.gene058263.t1.exon1;Parent=MS.gene058263.t1 |
| chr2.2 | CDS | 55144584 | 55144922 | 55144584 | ID=cds.MS.gene058263.t1;Parent=MS.gene058263.t1 |
| Gene Sequence |
| Protein sequence |
