AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67711

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67711.t1 MTR_2g033630 96.648 179 5 1 1 179 1 178 4.77e-125 349
MS.gene67711.t1 MTR_4g122740 66.298 181 57 3 1 179 1 179 2.99e-82 241
MS.gene67711.t1 MTR_4g122720 50.802 187 84 1 1 179 1 187 9.79e-64 194
MS.gene67711.t1 MTR_4g122718 50.893 112 52 1 68 179 1 109 6.26e-39 129
MS.gene67711.t1 MTR_4g122890 37.640 178 103 4 5 177 1 175 4.30e-27 100
MS.gene67711.t1 MTR_4g122712 33.516 182 106 4 1 176 1 173 2.97e-23 90.9
MS.gene67711.t1 MTR_4g122750 34.302 172 100 3 6 177 15 173 6.50e-21 85.1
MS.gene67711.t1 MTR_4g122800 31.579 171 104 3 16 177 16 182 2.38e-19 81.3
MS.gene67711.t1 MTR_4g122660 31.073 177 118 4 3 177 2 176 7.30e-18 77.4
MS.gene67711.t1 MTR_4g122760 32.192 146 97 1 32 177 33 176 1.09e-16 73.9
MS.gene67711.t1 MTR_2g033740 30.968 155 98 2 31 176 32 186 2.69e-16 73.2
MS.gene67711.t1 MTR_2g033720 30.968 155 98 2 31 176 32 186 4.45e-16 72.8
MS.gene67711.t1 MTR_3g095980 30.814 172 112 5 11 177 7 176 4.00e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67711.t1 AT3G17130 43.750 160 76 4 29 179 28 182 2.57e-38 130
MS.gene67711.t1 AT1G47960 35.616 146 90 2 32 177 25 166 1.86e-22 89.0
MS.gene67711.t1 AT1G47960 35.461 141 87 2 32 172 25 161 7.91e-20 82.8
MS.gene67711.t1 AT5G64620 27.586 174 113 5 17 178 8 180 4.99e-13 64.3
MS.gene67711.t1 AT3G17152 27.326 172 115 3 11 176 8 175 1.12e-12 63.5
MS.gene67711.t1 AT3G17150 27.119 177 126 2 1 177 8 181 1.25e-12 63.5

Find 27 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTGTTATGTCACCTTTA+GGG 0.220441 2.2:-55097091 MS.gene67711:CDS
GGGGTTGTTATGTCACCTTT+AGG 0.229357 2.2:-55097092 MS.gene67711:CDS
GAGCAAGTGGGTTTGAGTTT+AGG 0.264723 2.2:+55097390 None:intergenic
GCTATAAATCAAAACCGGTT+TGG 0.390286 2.2:-55097179 MS.gene67711:CDS
TATTATTAAACAACTATTGA+AGG 0.404982 2.2:-55097024 MS.gene67711:CDS
GGAGCTACATAGGTCGTAGA+AGG 0.415962 2.2:+55097415 None:intergenic
AAATGATGAAACAATGGTTA+TGG 0.416969 2.2:+55097526 None:intergenic
GAATGTTGTTTACCATTACT+AGG 0.433283 2.2:+55097342 None:intergenic
GAAAACTACAGATCGTGAAA+TGG 0.467104 2.2:-55097273 MS.gene67711:CDS
GGAAGAGTGTACTTCACAAT+TGG 0.475484 2.2:+55097215 None:intergenic
GGTTGTTATGTCACCTTTAG+GGG 0.492197 2.2:-55097090 MS.gene67711:CDS
AGTTTAGGATGGAGCTACAT+AGG 0.504787 2.2:+55097405 None:intergenic
CTGATGCTATAAATCAAAAC+CGG 0.505756 2.2:-55097184 MS.gene67711:CDS
ATGTGAAAGGTATGGAAGCA+AGG 0.520131 2.2:+55097483 None:intergenic
AATGTTGTTTACCATTACTA+GGG 0.522731 2.2:+55097343 None:intergenic
AATAACTAGCAAATCCAAAC+CGG 0.527876 2.2:+55097165 None:intergenic
TTGAGATGATGTGAAAGGTA+TGG 0.531143 2.2:+55097475 None:intergenic
ATACCATTTCTATCCCCTAA+AGG 0.536471 2.2:+55097077 None:intergenic
ATTATTAAACAACTATTGAA+GGG 0.542633 2.2:-55097023 MS.gene67711:CDS
TCACCTTTAGGGGATAGAAA+TGG 0.544897 2.2:-55097080 MS.gene67711:CDS
CAAAGGTGTAGCCCTAGTAA+TGG 0.545068 2.2:-55097354 MS.gene67711:CDS
TAGGGCTACACCTTTGAGAT+CGG 0.548198 2.2:+55097361 None:intergenic
TTTATAGCATCAGCAGCTTG+TGG 0.575094 2.2:+55097194 None:intergenic
TATTGCAAATGATGAAACAA+TGG 0.580448 2.2:+55097520 None:intergenic
AAGTGGGTTTGAGTTTAGGA+TGG 0.581840 2.2:+55097394 None:intergenic
GAGCTACATAGGTCGTAGAA+GGG 0.650219 2.2:+55097416 None:intergenic
TTGGATTGAGATGATGTGAA+AGG 0.681331 2.2:+55097470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTAAACAACTATTGAA+GGG - chr2.2:55097532-55097551 MS.gene67711:CDS 15.0%
!! TATTATTAAACAACTATTGA+AGG - chr2.2:55097531-55097550 MS.gene67711:CDS 15.0%
!!! TATTTTATCTATTAAATCGT+TGG + chr2.2:55097107-55097126 None:intergenic 15.0%
!!! CTTGTAATAAGAAATTTTCT+GGG - chr2.2:55097443-55097462 MS.gene67711:CDS 20.0%
!!! TCTTGTAATAAGAAATTTTC+TGG - chr2.2:55097442-55097461 MS.gene67711:CDS 20.0%
!!! TTGTAATAAGAAATTTTCTG+GGG - chr2.2:55097444-55097463 MS.gene67711:CDS 20.0%
! AAATGATGAAACAATGGTTA+TGG + chr2.2:55097032-55097051 None:intergenic 25.0%
! TATTGCAAATGATGAAACAA+TGG + chr2.2:55097038-55097057 None:intergenic 25.0%
!! AATGTTGTTTACCATTACTA+GGG + chr2.2:55097215-55097234 None:intergenic 25.0%
AATAACTAGCAAATCCAAAC+CGG + chr2.2:55097393-55097412 None:intergenic 30.0%
CTGATGCTATAAATCAAAAC+CGG - chr2.2:55097371-55097390 MS.gene67711:CDS 30.0%
! GAATGTTGTTTACCATTACT+AGG + chr2.2:55097216-55097235 None:intergenic 30.0%
ATACCATTTCTATCCCCTAA+AGG + chr2.2:55097481-55097500 None:intergenic 35.0%
GAAAACTACAGATCGTGAAA+TGG - chr2.2:55097282-55097301 MS.gene67711:CDS 35.0%
GCTATAAATCAAAACCGGTT+TGG - chr2.2:55097376-55097395 MS.gene67711:CDS 35.0%
TTGAGATGATGTGAAAGGTA+TGG + chr2.2:55097083-55097102 None:intergenic 35.0%
TTGGATTGAGATGATGTGAA+AGG + chr2.2:55097088-55097107 None:intergenic 35.0%
! TCGTGAAATGGAAAAAGCAT+TGG - chr2.2:55097294-55097313 MS.gene67711:CDS 35.0%
GGGTTGTTATGTCACCTTTA+GGG - chr2.2:55097464-55097483 MS.gene67711:CDS 40.0%
GGTTGTTATGTCACCTTTAG+GGG - chr2.2:55097465-55097484 MS.gene67711:CDS 40.0%
TTTATAGCATCAGCAGCTTG+TGG + chr2.2:55097364-55097383 None:intergenic 40.0%
! AAGTGGGTTTGAGTTTAGGA+TGG + chr2.2:55097164-55097183 None:intergenic 40.0%
! AGTTTAGGATGGAGCTACAT+AGG + chr2.2:55097153-55097172 None:intergenic 40.0%
! ATGTGAAAGGTATGGAAGCA+AGG + chr2.2:55097075-55097094 None:intergenic 40.0%
! GGAAGAGTGTACTTCACAAT+TGG + chr2.2:55097343-55097362 None:intergenic 40.0%
! TCACCTTTAGGGGATAGAAA+TGG - chr2.2:55097475-55097494 MS.gene67711:CDS 40.0%
!!! ACACCTTTGAGATCGGTTTT+TGG + chr2.2:55097190-55097209 None:intergenic 40.0%
GAGCTACATAGGTCGTAGAA+GGG + chr2.2:55097142-55097161 None:intergenic 45.0%
GCTCCAAAAACCGATCTCAA+AGG - chr2.2:55097184-55097203 MS.gene67711:CDS 45.0%
GGGGTTGTTATGTCACCTTT+AGG - chr2.2:55097463-55097482 MS.gene67711:CDS 45.0%
! CAAAGGTGTAGCCCTAGTAA+TGG - chr2.2:55097201-55097220 MS.gene67711:CDS 45.0%
! GAGCAAGTGGGTTTGAGTTT+AGG + chr2.2:55097168-55097187 None:intergenic 45.0%
!! TAGGGCTACACCTTTGAGAT+CGG + chr2.2:55097197-55097216 None:intergenic 45.0%
!!! AGATCGGTTTTTGGAGCAAG+TGG + chr2.2:55097181-55097200 None:intergenic 45.0%
!!! GATCGGTTTTTGGAGCAAGT+GGG + chr2.2:55097180-55097199 None:intergenic 45.0%
GGAGCTACATAGGTCGTAGA+AGG + chr2.2:55097143-55097162 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 55097019 55097558 55097019 ID=MS.gene67711
chr2.2 mRNA 55097019 55097558 55097019 ID=MS.gene67711.t1;Parent=MS.gene67711
chr2.2 exon 55097019 55097558 55097019 ID=MS.gene67711.t1.exon1;Parent=MS.gene67711.t1
chr2.2 CDS 55097019 55097558 55097019 ID=cds.MS.gene67711.t1;Parent=MS.gene67711.t1
Gene Sequence

>MS.gene67711

ATGAGAAAAACCATAACCATTGTTTCATCATTTGCAATAATTCACATTATTCTCCTTGCTTCCATACCTTTCACATCATCTCAATCCAACGATTTAATAGATAAAATATGCAAAAAAACACCCTTCTACGACCTATGTAGCTCCATCCTAAACTCAAACCCACTTGCTCCAAAAACCGATCTCAAAGGTGTAGCCCTAGTAATGGTAAACAACATTCTCTCAAATGCAAGTGACACATTGAACTACATTGAAAGCTTGATCAAGAAAACTACAGATCGTGAAATGGAAAAAGCATTGGCATTTTGTGCTGAATCATATGTACCAATTGTGAAGTACACTCTTCCACAAGCTGCTGATGCTATAAATCAAAACCGGTTTGGATTTGCTAGTTATTGTATTTCTGATGCTGTTAAAGAAGTTAATTCTTGTAATAAGAAATTTTCTGGGGTTGTTATGTCACCTTTAGGGGATAGAAATGGTATTGTTCAGAAGCTTGTTGATGTTGCTTCTGCTATTATTAAACAACTATTGAAGGGTTGA
Protein sequence

>MS.gene67711.t1

MRKTITIVSSFAIIHIILLASIPFTSSQSNDLIDKICKKTPFYDLCSSILNSNPLAPKTDLKGVALVMVNNILSNASDTLNYIESLIKKTTDREMEKALAFCAESYVPIVKYTLPQAADAINQNRFGFASYCISDAVKEVNSCNKKFSGVVMSPLGDRNGIVQKLVDVASAIIKQLLKG