Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058422.t1 | XP_027333625.1 | 82.9 | 76 | 12 | 1 | 6 | 81 | 169 | 243 | 5.00E-31 | 143.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058422.t1 | Q5XEM9 | 87.9 | 58 | 7 | 0 | 24 | 81 | 203 | 260 | 5.8e-28 | 124.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058422.t1 | A0A3N7F8Q0 | 77.9 | 77 | 17 | 0 | 5 | 81 | 163 | 239 | 1.0e-30 | 141.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene058422.t1 | TF | Alfin-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058422 | MS.gene33050 | 0.87496 | 4.49E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058422.t1 | MTR_1g015185 | 78.824 | 85 | 14 | 1 | 1 | 81 | 179 | 263 | 6.78e-37 | 124 |
| MS.gene058422.t1 | MTR_8g019520 | 88.333 | 60 | 6 | 1 | 23 | 81 | 198 | 257 | 3.59e-34 | 117 |
| MS.gene058422.t1 | MTR_8g019520 | 88.333 | 60 | 6 | 1 | 23 | 81 | 197 | 256 | 3.71e-34 | 117 |
| MS.gene058422.t1 | MTR_2g099500 | 87.931 | 58 | 6 | 1 | 24 | 80 | 193 | 250 | 2.76e-31 | 109 |
| MS.gene058422.t1 | MTR_2g040990 | 83.929 | 56 | 7 | 1 | 28 | 81 | 198 | 253 | 1.21e-29 | 105 |
| MS.gene058422.t1 | MTR_4g015830 | 67.089 | 79 | 25 | 1 | 4 | 81 | 162 | 240 | 6.89e-29 | 103 |
| MS.gene058422.t1 | MTR_4g134510 | 81.132 | 53 | 10 | 0 | 28 | 80 | 202 | 254 | 3.27e-28 | 102 |
| MS.gene058422.t1 | MTR_7g068590 | 75.000 | 56 | 14 | 0 | 26 | 81 | 187 | 242 | 6.36e-27 | 98.2 |
| MS.gene058422.t1 | MTR_8g066480 | 54.545 | 55 | 12 | 2 | 28 | 81 | 104 | 146 | 1.75e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058422.t1 | AT5G20510 | 88.136 | 59 | 7 | 0 | 23 | 81 | 161 | 219 | 3.05e-36 | 121 |
| MS.gene058422.t1 | AT5G20510 | 88.136 | 59 | 7 | 0 | 23 | 81 | 202 | 260 | 6.03e-36 | 122 |
| MS.gene058422.t1 | AT3G42790 | 91.379 | 58 | 5 | 0 | 23 | 80 | 192 | 249 | 2.25e-35 | 120 |
| MS.gene058422.t1 | AT5G26210 | 89.091 | 55 | 6 | 0 | 26 | 80 | 200 | 254 | 1.29e-31 | 110 |
| MS.gene058422.t1 | AT2G02470 | 83.636 | 55 | 9 | 0 | 27 | 81 | 202 | 256 | 6.98e-31 | 108 |
| MS.gene058422.t1 | AT2G02470 | 83.636 | 55 | 9 | 0 | 27 | 81 | 193 | 247 | 7.67e-31 | 108 |
| MS.gene058422.t1 | AT5G05610 | 59.740 | 77 | 31 | 0 | 4 | 80 | 164 | 240 | 2.04e-28 | 102 |
| MS.gene058422.t1 | AT5G05610 | 59.740 | 77 | 31 | 0 | 4 | 80 | 164 | 240 | 2.04e-28 | 102 |
| MS.gene058422.t1 | AT3G11200 | 68.966 | 58 | 18 | 0 | 23 | 80 | 188 | 245 | 2.04e-26 | 97.1 |
| MS.gene058422.t1 | AT3G11200 | 68.966 | 58 | 18 | 0 | 23 | 80 | 175 | 232 | 2.28e-26 | 96.7 |
| MS.gene058422.t1 | AT1G14510 | 75.000 | 52 | 13 | 0 | 27 | 78 | 197 | 248 | 8.35e-26 | 95.9 |
Find 21 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATGCAGCTGATGAGTTC+TGG | 0.348702 | 1.4:+6823563 | MS.gene058422:CDS |
| TTGATATGCTCAGCCCTTGC+CGG | 0.382099 | 1.4:-6823640 | None:intergenic |
| GCAGTGACATTTGCGAGAAA+TGG | 0.391588 | 1.4:+6823590 | MS.gene058422:CDS |
| ATTTGCGAGAAATGGTTTCA+TGG | 0.414893 | 1.4:+6823598 | MS.gene058422:CDS |
| GTCAAAGGACGAGGAGGTTG+AGG | 0.459920 | 1.4:+6823480 | MS.gene058422:CDS |
| GCTCTCTTGTTGCTGCAAGC+TGG | 0.467608 | 1.4:-6823676 | None:intergenic |
| CGTGAAGATTACTCCGGCAA+GGG | 0.576974 | 1.4:+6823627 | MS.gene058422:CDS |
| GGTTGAGGATGGTGTAGGTG+TGG | 0.578977 | 1.4:+6823495 | MS.gene058422:CDS |
| GAGGAGGTTGAGGATGGTGT+AGG | 0.579309 | 1.4:+6823490 | MS.gene058422:CDS |
| GCGTGAAGATTACTCCGGCA+AGG | 0.589663 | 1.4:+6823626 | MS.gene058422:CDS |
| GGCAATGCAAATGAAGTCAA+AGG | 0.599376 | 1.4:+6823465 | MS.gene058422:CDS |
| GCAAATGAAGTCAAAGGACG+AGG | 0.610740 | 1.4:+6823471 | MS.gene058422:CDS |
| GAGACTTTGTGTGGGGCATG+TGG | 0.613065 | 1.4:+6823535 | MS.gene058422:CDS |
| AAGGACGAGGAGGTTGAGGA+TGG | 0.620188 | 1.4:+6823484 | MS.gene058422:CDS |
| AACACGGAGAGACTTTGTGT+GGG | 0.622939 | 1.4:+6823527 | MS.gene058422:CDS |
| GGATGGTGTAGGTGTGGACG+AGG | 0.628142 | 1.4:+6823501 | MS.gene058422:CDS |
| GAACACGGAGAGACTTTGTG+TGG | 0.630911 | 1.4:+6823526 | MS.gene058422:CDS |
| ACACGGAGAGACTTTGTGTG+GGG | 0.707279 | 1.4:+6823528 | MS.gene058422:CDS |
| AATGAAGTCAAAGGACGAGG+AGG | 0.714948 | 1.4:+6823474 | MS.gene058422:CDS |
| AAAATGCGTGAAGATTACTC+CGG | 0.724223 | 1.4:+6823621 | MS.gene058422:CDS |
| GGTGTGGACGAGGAAGAACA+CGG | 0.729144 | 1.4:+6823511 | MS.gene058422:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGCGTGAAGATTACTC+CGG | + | chr1.4:6823621-6823640 | MS.gene058422:CDS | 35.0% | |
| ATTTGCGAGAAATGGTTTCA+TGG | + | chr1.4:6823598-6823617 | MS.gene058422:CDS | 35.0% | |
| GGCAATGCAAATGAAGTCAA+AGG | + | chr1.4:6823465-6823484 | MS.gene058422:CDS | 40.0% | |
| AACACGGAGAGACTTTGTGT+GGG | + | chr1.4:6823527-6823546 | MS.gene058422:CDS | 45.0% | |
| AATGAAGTCAAAGGACGAGG+AGG | + | chr1.4:6823474-6823493 | MS.gene058422:CDS | 45.0% | |
| GCAAATGAAGTCAAAGGACG+AGG | + | chr1.4:6823471-6823490 | MS.gene058422:CDS | 45.0% | |
| GCAGTGACATTTGCGAGAAA+TGG | + | chr1.4:6823590-6823609 | MS.gene058422:CDS | 45.0% | |
| ACACGGAGAGACTTTGTGTG+GGG | + | chr1.4:6823528-6823547 | MS.gene058422:CDS | 50.0% | |
| CGTGAAGATTACTCCGGCAA+GGG | + | chr1.4:6823627-6823646 | MS.gene058422:CDS | 50.0% | |
| GAACACGGAGAGACTTTGTG+TGG | + | chr1.4:6823526-6823545 | MS.gene058422:CDS | 50.0% | |
| GCTATGCAGCTGATGAGTTC+TGG | + | chr1.4:6823563-6823582 | MS.gene058422:CDS | 50.0% | |
| TTGATATGCTCAGCCCTTGC+CGG | - | chr1.4:6823643-6823662 | None:intergenic | 50.0% | |
| AAGGACGAGGAGGTTGAGGA+TGG | + | chr1.4:6823484-6823503 | MS.gene058422:CDS | 55.0% | |
| GAGACTTTGTGTGGGGCATG+TGG | + | chr1.4:6823535-6823554 | MS.gene058422:CDS | 55.0% | |
| GAGGAGGTTGAGGATGGTGT+AGG | + | chr1.4:6823490-6823509 | MS.gene058422:CDS | 55.0% | |
| GCGTGAAGATTACTCCGGCA+AGG | + | chr1.4:6823626-6823645 | MS.gene058422:CDS | 55.0% | |
| GCTCTCTTGTTGCTGCAAGC+TGG | - | chr1.4:6823679-6823698 | None:intergenic | 55.0% | |
| GGTGTGGACGAGGAAGAACA+CGG | + | chr1.4:6823511-6823530 | MS.gene058422:CDS | 55.0% | |
| GGTTGAGGATGGTGTAGGTG+TGG | + | chr1.4:6823495-6823514 | MS.gene058422:CDS | 55.0% | |
| GTCAAAGGACGAGGAGGTTG+AGG | + | chr1.4:6823480-6823499 | MS.gene058422:CDS | 55.0% | |
| GGATGGTGTAGGTGTGGACG+AGG | + | chr1.4:6823501-6823520 | MS.gene058422:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 6823463 | 6823708 | 6823463 | ID=MS.gene058422 |
| chr1.4 | mRNA | 6823463 | 6823708 | 6823463 | ID=MS.gene058422.t1;Parent=MS.gene058422 |
| chr1.4 | exon | 6823463 | 6823708 | 6823463 | ID=MS.gene058422.t1.exon1;Parent=MS.gene058422.t1 |
| chr1.4 | CDS | 6823463 | 6823708 | 6823463 | ID=cds.MS.gene058422.t1;Parent=MS.gene058422.t1 |
| Gene Sequence |
| Protein sequence |
