Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33050.t1 | XP_022723850.1 | 54 | 126 | 58 | 0 | 1 | 126 | 126 | 251 | 1.70E-30 | 142.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33050.t1 | Q5XEM9 | 87.9 | 58 | 7 | 0 | 69 | 126 | 203 | 260 | 9.0e-28 | 124.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33050.t1 | A0A371GC45 | 54.8 | 126 | 57 | 0 | 1 | 126 | 161 | 286 | 1.3e-30 | 142.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene33050.t1 | TF | Alfin-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058422 | MS.gene33050 | 0.87496 | 4.49E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33050.t1 | MTR_1g015185 | 70.073 | 137 | 30 | 2 | 1 | 126 | 127 | 263 | 6.49e-55 | 172 |
| MS.gene33050.t1 | MTR_8g019520 | 68.702 | 131 | 36 | 2 | 1 | 126 | 126 | 256 | 2.03e-46 | 150 |
| MS.gene33050.t1 | MTR_8g019520 | 61.314 | 137 | 37 | 3 | 1 | 126 | 126 | 257 | 1.74e-45 | 148 |
| MS.gene33050.t1 | MTR_2g040990 | 64.844 | 128 | 40 | 3 | 1 | 126 | 129 | 253 | 2.91e-45 | 147 |
| MS.gene33050.t1 | MTR_2g099500 | 65.116 | 129 | 41 | 2 | 1 | 125 | 122 | 250 | 1.90e-40 | 135 |
| MS.gene33050.t1 | MTR_4g134510 | 54.615 | 130 | 52 | 4 | 1 | 125 | 127 | 254 | 7.21e-38 | 129 |
| MS.gene33050.t1 | MTR_4g015830 | 51.969 | 127 | 52 | 2 | 1 | 126 | 122 | 240 | 1.37e-34 | 120 |
| MS.gene33050.t1 | MTR_7g068590 | 51.562 | 128 | 53 | 5 | 1 | 126 | 122 | 242 | 1.09e-31 | 112 |
| MS.gene33050.t1 | MTR_8g066480 | 51.587 | 126 | 45 | 4 | 2 | 126 | 36 | 146 | 1.58e-24 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33050.t1 | AT3G42790 | 65.600 | 125 | 41 | 1 | 1 | 125 | 127 | 249 | 6.31e-48 | 154 |
| MS.gene33050.t1 | AT5G20510 | 60.448 | 134 | 45 | 2 | 1 | 126 | 86 | 219 | 9.88e-47 | 150 |
| MS.gene33050.t1 | AT5G20510 | 60.448 | 134 | 45 | 2 | 1 | 126 | 127 | 260 | 2.10e-46 | 150 |
| MS.gene33050.t1 | AT5G26210 | 65.414 | 133 | 34 | 2 | 1 | 125 | 126 | 254 | 5.78e-40 | 134 |
| MS.gene33050.t1 | AT2G02470 | 55.303 | 132 | 52 | 3 | 1 | 126 | 126 | 256 | 5.25e-39 | 131 |
| MS.gene33050.t1 | AT2G02470 | 57.143 | 126 | 50 | 3 | 1 | 126 | 126 | 247 | 2.14e-38 | 130 |
| MS.gene33050.t1 | AT5G05610 | 50.794 | 126 | 53 | 3 | 1 | 125 | 123 | 240 | 1.60e-34 | 119 |
| MS.gene33050.t1 | AT5G05610 | 50.794 | 126 | 53 | 3 | 1 | 125 | 123 | 240 | 1.60e-34 | 119 |
| MS.gene33050.t1 | AT1G14510 | 50.806 | 124 | 59 | 2 | 1 | 123 | 126 | 248 | 2.12e-33 | 117 |
| MS.gene33050.t1 | AT3G11200 | 47.656 | 128 | 57 | 2 | 1 | 125 | 125 | 245 | 1.14e-32 | 115 |
| MS.gene33050.t1 | AT3G11200 | 47.656 | 128 | 57 | 2 | 1 | 125 | 112 | 232 | 1.42e-32 | 114 |
Find 31 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGCATTGCCATTGATAT+TGG | 0.306155 | 1.1:-6596127 | None:intergenic |
| GCTATGCAGCTGATGAGTTC+TGG | 0.348702 | 1.1:+6596236 | MS.gene33050:CDS |
| ACAATCTTTGAAGCTGTTGC+AGG | 0.355548 | 1.1:+6595437 | MS.gene33050:CDS |
| AAGTCATCAGTTTCTAATAA+TGG | 0.364864 | 1.1:+6595485 | MS.gene33050:CDS |
| TTGATATGCTCAGCCCTTGC+CGG | 0.382099 | 1.1:-6596313 | None:intergenic |
| TCTTACCTTAGAGTTTGATT+TGG | 0.384520 | 1.1:-6595519 | None:intergenic |
| TTTGAAGCTGTTGCAGGGTC+TGG | 0.385521 | 1.1:+6595443 | MS.gene33050:CDS |
| GCAGTGACATTTGCGAGAAA+TGG | 0.391588 | 1.1:+6596263 | MS.gene33050:CDS |
| GAAATCCAAATCAAACTCTA+AGG | 0.408549 | 1.1:+6595514 | MS.gene33050:CDS |
| ATTTGCGAGAAATGGTTTCA+TGG | 0.414893 | 1.1:+6596271 | MS.gene33050:CDS |
| GCAACAGCTTCAAAGATTGT+TGG | 0.414898 | 1.1:-6595434 | None:intergenic |
| GTCAAAGGACGAGGAGGTTG+AGG | 0.459920 | 1.1:+6596153 | MS.gene33050:CDS |
| GCTCTCTTGTTGCTGCAAGC+TGG | 0.467608 | 1.1:-6596349 | None:intergenic |
| GCAGGGTCTGGTAGCAAACA+AGG | 0.540889 | 1.1:+6595455 | MS.gene33050:CDS |
| CAATCTTTGAAGCTGTTGCA+GGG | 0.574674 | 1.1:+6595438 | MS.gene33050:CDS |
| GTCTGGTAGCAAACAAGGAA+AGG | 0.575473 | 1.1:+6595460 | MS.gene33050:CDS |
| CGTGAAGATTACTCCGGCAA+GGG | 0.576974 | 1.1:+6596300 | MS.gene33050:CDS |
| GGTTGAGGATGGTGTAGGTG+TGG | 0.578977 | 1.1:+6596168 | MS.gene33050:CDS |
| GAGGAGGTTGAGGATGGTGT+AGG | 0.579309 | 1.1:+6596163 | MS.gene33050:CDS |
| GCGTGAAGATTACTCCGGCA+AGG | 0.589663 | 1.1:+6596299 | MS.gene33050:CDS |
| GGCAATGCAAATGAAGTCAA+AGG | 0.599376 | 1.1:+6596138 | MS.gene33050:CDS |
| GCAAATGAAGTCAAAGGACG+AGG | 0.610740 | 1.1:+6596144 | MS.gene33050:CDS |
| GAGACTTTGTGTGGGGCATG+TGG | 0.613065 | 1.1:+6596208 | MS.gene33050:CDS |
| AAGGACGAGGAGGTTGAGGA+TGG | 0.620188 | 1.1:+6596157 | MS.gene33050:CDS |
| AACACGGAGAGACTTTGTGT+GGG | 0.622939 | 1.1:+6596200 | MS.gene33050:CDS |
| GGATGGTGTAGGTGTGGACG+AGG | 0.628142 | 1.1:+6596174 | MS.gene33050:CDS |
| GAACACGGAGAGACTTTGTG+TGG | 0.630911 | 1.1:+6596199 | MS.gene33050:CDS |
| ACACGGAGAGACTTTGTGTG+GGG | 0.707279 | 1.1:+6596201 | MS.gene33050:CDS |
| AATGAAGTCAAAGGACGAGG+AGG | 0.714948 | 1.1:+6596147 | MS.gene33050:CDS |
| AAAATGCGTGAAGATTACTC+CGG | 0.724223 | 1.1:+6596294 | MS.gene33050:CDS |
| GGTGTGGACGAGGAAGAACA+CGG | 0.729144 | 1.1:+6596184 | MS.gene33050:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGCAAATTATTAACATCAA+TGG | - | chr1.1:6595572-6595591 | None:intergenic | 20.0% |
| !!! | ATTGATGTTAATAATTTGCT+AGG | + | chr1.1:6595571-6595590 | MS.gene33050:intron | 20.0% |
| ! | AAGTCATCAGTTTCTAATAA+TGG | + | chr1.1:6595485-6595504 | MS.gene33050:CDS | 25.0% |
| ! | GTCATTTAATCTTGATTGAA+AGG | - | chr1.1:6595731-6595750 | None:intergenic | 25.0% |
| ! | GTTTGGTATTTAATCAGTTA+AGG | + | chr1.1:6596042-6596061 | MS.gene33050:intron | 25.0% |
| ! | TTTATAGTTCAAGAACGTAT+TGG | + | chr1.1:6595936-6595955 | MS.gene33050:intron | 25.0% |
| !! | GTTTTCAAAACCAATATCAA+TGG | + | chr1.1:6596117-6596136 | MS.gene33050:intron | 25.0% |
| !!! | GAAAGTGAAAAGTATTTTGA+GGG | + | chr1.1:6595648-6595667 | MS.gene33050:intron | 25.0% |
| !!! | GATATTGGTTTTGAAAACTT+TGG | - | chr1.1:6596115-6596134 | None:intergenic | 25.0% |
| ATTTGCATTGCCATTGATAT+TGG | - | chr1.1:6596130-6596149 | None:intergenic | 30.0% | |
| GAAATCCAAATCAAACTCTA+AGG | + | chr1.1:6595514-6595533 | MS.gene33050:CDS | 30.0% | |
| GATTAAATACCAAACCAATC+AGG | - | chr1.1:6596037-6596056 | None:intergenic | 30.0% | |
| TCTTACCTTAGAGTTTGATT+TGG | - | chr1.1:6595522-6595541 | None:intergenic | 30.0% | |
| ! | CAAATCTGAAGTCATAATGT+GGG | - | chr1.1:6595753-6595772 | None:intergenic | 30.0% |
| ! | TCAAATCTGAAGTCATAATG+TGG | - | chr1.1:6595754-6595773 | None:intergenic | 30.0% |
| !!! | CGAAAGTGAAAAGTATTTTG+AGG | + | chr1.1:6595647-6595666 | MS.gene33050:intron | 30.0% |
| AAAATGCGTGAAGATTACTC+CGG | + | chr1.1:6596294-6596313 | MS.gene33050:CDS | 35.0% | |
| ACTTTCTTGCTCCAAAAACA+AGG | - | chr1.1:6595914-6595933 | None:intergenic | 35.0% | |
| ATTTGCGAGAAATGGTTTCA+TGG | + | chr1.1:6596271-6596290 | MS.gene33050:CDS | 35.0% | |
| CTTGACCATAAATATCCAGA+CGG | - | chr1.1:6595688-6595707 | None:intergenic | 35.0% | |
| TGACCATATATCCCTGTTTA+TGG | + | chr1.1:6595975-6595994 | MS.gene33050:intron | 35.0% | |
| TTGCTCCAAAAACAAGGATT+CGG | - | chr1.1:6595908-6595927 | None:intergenic | 35.0% | |
| ! | AGTCGCGTAATATGTTGTTT+AGG | + | chr1.1:6595606-6595625 | MS.gene33050:intron | 35.0% |
| ! | GTTAAGGCTGTTATCTCTTT+TGG | + | chr1.1:6596058-6596077 | MS.gene33050:intron | 35.0% |
| !!! | GGTTTTGAAAACTTTGGTTG+AGG | - | chr1.1:6596109-6596128 | None:intergenic | 35.0% |
| GAATCTGTCTGTTTCCTGAT+TGG | + | chr1.1:6596020-6596039 | MS.gene33050:intron | 40.0% | |
| GCAACAGCTTCAAAGATTGT+TGG | - | chr1.1:6595437-6595456 | None:intergenic | 40.0% | |
| GGCAATGCAAATGAAGTCAA+AGG | + | chr1.1:6596138-6596157 | MS.gene33050:CDS | 40.0% | |
| TGAAAGGTTATCAGAGAGTG+TGG | - | chr1.1:6595715-6595734 | None:intergenic | 40.0% | |
| ! | ACTTTCTAGCGCCATAAACA+GGG | - | chr1.1:6595989-6596008 | None:intergenic | 40.0% |
| ! | TGTCTGTTTCCTGATTGGTT+TGG | + | chr1.1:6596025-6596044 | MS.gene33050:intron | 40.0% |
| !! | ACAATCTTTGAAGCTGTTGC+AGG | + | chr1.1:6595437-6595456 | MS.gene33050:CDS | 40.0% |
| !! | CAATCTTTGAAGCTGTTGCA+GGG | + | chr1.1:6595438-6595457 | MS.gene33050:CDS | 40.0% |
| !! | TAGTCTAGTTTGCTTGACTG+TGG | + | chr1.1:6595852-6595871 | MS.gene33050:intron | 40.0% |
| !!! | GAGATCCGAATCCTTGTTTT+TGG | + | chr1.1:6595900-6595919 | MS.gene33050:intron | 40.0% |
| AACACGGAGAGACTTTGTGT+GGG | + | chr1.1:6596200-6596219 | MS.gene33050:CDS | 45.0% | |
| AATGAAGTCAAAGGACGAGG+AGG | + | chr1.1:6596147-6596166 | MS.gene33050:CDS | 45.0% | |
| ACCATAAATATCCAGACGGC+AGG | - | chr1.1:6595684-6595703 | None:intergenic | 45.0% | |
| ATGTGTGAATCCTTTGCTGC+TGG | + | chr1.1:6595878-6595897 | MS.gene33050:intron | 45.0% | |
| CCATAAATATCCAGACGGCA+GGG | - | chr1.1:6595683-6595702 | None:intergenic | 45.0% | |
| GCAAATGAAGTCAAAGGACG+AGG | + | chr1.1:6596144-6596163 | MS.gene33050:CDS | 45.0% | |
| GCAGTGACATTTGCGAGAAA+TGG | + | chr1.1:6596263-6596282 | MS.gene33050:CDS | 45.0% | |
| GCGCCATAAACAGGGATATA+TGG | - | chr1.1:6595981-6596000 | None:intergenic | 45.0% | |
| GTCTGGTAGCAAACAAGGAA+AGG | + | chr1.1:6595460-6595479 | MS.gene33050:CDS | 45.0% | |
| ! | GACTTTCTAGCGCCATAAAC+AGG | - | chr1.1:6595990-6596009 | None:intergenic | 45.0% |
| ACACGGAGAGACTTTGTGTG+GGG | + | chr1.1:6596201-6596220 | MS.gene33050:CDS | 50.0% | |
| ATTCGGATCTCCAGCAGCAA+AGG | - | chr1.1:6595891-6595910 | None:intergenic | 50.0% | |
| CCCTGCCGTCTGGATATTTA+TGG | + | chr1.1:6595680-6595699 | MS.gene33050:intron | 50.0% | |
| CGTGAAGATTACTCCGGCAA+GGG | + | chr1.1:6596300-6596319 | MS.gene33050:CDS | 50.0% | |
| GAACACGGAGAGACTTTGTG+TGG | + | chr1.1:6596199-6596218 | MS.gene33050:CDS | 50.0% | |
| GATTTAGATTCCCTGCCGTC+TGG | + | chr1.1:6595670-6595689 | MS.gene33050:intron | 50.0% | |
| GCTATGCAGCTGATGAGTTC+TGG | + | chr1.1:6596236-6596255 | MS.gene33050:CDS | 50.0% | |
| TTGATATGCTCAGCCCTTGC+CGG | - | chr1.1:6596316-6596335 | None:intergenic | 50.0% | |
| ! | TTTGAAGCTGTTGCAGGGTC+TGG | + | chr1.1:6595443-6595462 | MS.gene33050:CDS | 50.0% |
| AAGGACGAGGAGGTTGAGGA+TGG | + | chr1.1:6596157-6596176 | MS.gene33050:CDS | 55.0% | |
| GAGACTTTGTGTGGGGCATG+TGG | + | chr1.1:6596208-6596227 | MS.gene33050:CDS | 55.0% | |
| GAGGAGGTTGAGGATGGTGT+AGG | + | chr1.1:6596163-6596182 | MS.gene33050:CDS | 55.0% | |
| GCAGGGTCTGGTAGCAAACA+AGG | + | chr1.1:6595455-6595474 | MS.gene33050:CDS | 55.0% | |
| GCGTGAAGATTACTCCGGCA+AGG | + | chr1.1:6596299-6596318 | MS.gene33050:CDS | 55.0% | |
| GCTCTCTTGTTGCTGCAAGC+TGG | - | chr1.1:6596352-6596371 | None:intergenic | 55.0% | |
| GGTGTGGACGAGGAAGAACA+CGG | + | chr1.1:6596184-6596203 | MS.gene33050:CDS | 55.0% | |
| GGTTGAGGATGGTGTAGGTG+TGG | + | chr1.1:6596168-6596187 | MS.gene33050:CDS | 55.0% | |
| GTCAAAGGACGAGGAGGTTG+AGG | + | chr1.1:6596153-6596172 | MS.gene33050:CDS | 55.0% | |
| GGATGGTGTAGGTGTGGACG+AGG | + | chr1.1:6596174-6596193 | MS.gene33050:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 6595419 | 6596381 | 6595419 | ID=MS.gene33050 |
| chr1.1 | mRNA | 6595419 | 6596381 | 6595419 | ID=MS.gene33050.t1;Parent=MS.gene33050 |
| chr1.1 | exon | 6595419 | 6595535 | 6595419 | ID=MS.gene33050.t1.exon1;Parent=MS.gene33050.t1 |
| chr1.1 | CDS | 6595419 | 6595535 | 6595419 | ID=cds.MS.gene33050.t1;Parent=MS.gene33050.t1 |
| chr1.1 | exon | 6596118 | 6596381 | 6596118 | ID=MS.gene33050.t1.exon2;Parent=MS.gene33050.t1 |
| chr1.1 | CDS | 6596118 | 6596381 | 6596118 | ID=cds.MS.gene33050.t1;Parent=MS.gene33050.t1 |
| Gene Sequence |
| Protein sequence |
