Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058522.t1 | XP_003624053.1 | 98.9 | 181 | 2 | 0 | 1 | 181 | 49 | 229 | 4.80E-98 | 367.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058522.t1 | Q3EDL4 | 67.6 | 185 | 45 | 6 | 1 | 181 | 49 | 222 | 3.0e-56 | 219.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058522.t1 | G7L6L4 | 98.9 | 181 | 2 | 0 | 1 | 181 | 49 | 229 | 3.4e-98 | 367.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058521 | MS.gene058522 | 0.888848 | 4.10E-73 | -1.69E-46 |
| MS.gene058522 | MS.gene058523 | 0.943745 | 6.91E-103 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATAAAAAGTTAAAAG+TGG | + | 50230:1702-1721 | MS.gene058522:intergenic | 10.0% |
| !! | TAAAAGTAAAAAATATTGAA+AGG | + | 50230:1869-1888 | MS.gene058522:intergenic | 10.0% |
| !!! | TTCTATAATTTTAATGTTTA+AGG | - | 50230:1525-1544 | MS.gene058522:intron | 10.0% |
| !! | ATTTACTATTAGTATTTGTT+TGG | - | 50230:928-947 | MS.gene058522:intron | 15.0% |
| !! | GAAAGATAATGAAATTTAAA+TGG | - | 50230:1183-1202 | MS.gene058522:intron | 15.0% |
| !! | TTTAATAAAGCATAATCTAA+TGG | + | 50230:1244-1263 | MS.gene058522:intergenic | 15.0% |
| !!! | TATTTTTGAAATTCATTGTT+TGG | - | 50230:671-690 | MS.gene058522:intron | 15.0% |
| !! | AAATGAATTTAACCAAATCA+AGG | + | 50230:1060-1079 | MS.gene058522:intergenic | 20.0% |
| !! | TGAAAGTAAAATGAAAATAC+TGG | + | 50230:1670-1689 | MS.gene058522:intergenic | 20.0% |
| !! | TTAAGAGTTATCATTTAAGT+GGG | - | 50230:1744-1763 | MS.gene058522:CDS | 20.0% |
| !!! | AATTGATTATTTTCATCACA+TGG | + | 50230:1782-1801 | MS.gene058522:intergenic | 20.0% |
| !!! | CATCAACTTTTTTTTTATGT+AGG | - | 50230:1644-1663 | MS.gene058522:intron | 20.0% |
| !!! | GAATTTTTATTTGTTCTCTT+TGG | - | 50230:1128-1147 | MS.gene058522:intron | 20.0% |
| !!! | TAATGTTTAACTTCTTCATA+GGG | - | 50230:1964-1983 | MS.gene058522:CDS | 20.0% |
| !!! | TGTTTTTATGTACTTGAATT+TGG | - | 50230:709-728 | MS.gene058522:intron | 20.0% |
| !!! | TTAATGTTTAACTTCTTCAT+AGG | - | 50230:1963-1982 | MS.gene058522:CDS | 20.0% |
| ! | ACAAACTATACATAGAAAAC+AGG | + | 50230:760-779 | MS.gene058522:intergenic | 25.0% |
| ! | ATAGAAGATGCAATTAATCA+TGG | + | 50230:1268-1287 | MS.gene058522:intergenic | 25.0% |
| ! | CACTTAAATGATAACTCTTA+AGG | + | 50230:1745-1764 | MS.gene058522:intergenic | 25.0% |
| ! | CTTAAGAGTTATCATTTAAG+TGG | - | 50230:1743-1762 | MS.gene058522:CDS | 25.0% |
| ! | TAAGAGTTATCATTTAAGTG+GGG | - | 50230:1745-1764 | MS.gene058522:CDS | 25.0% |
| !! | AATGTTTAACTTCTTCATAG+GGG | - | 50230:1965-1984 | MS.gene058522:CDS | 25.0% |
| !!! | ACATTTGTTTTATCATTCCA+TGG | + | 50230:1379-1398 | MS.gene058522:intergenic | 25.0% |
| !!! | TCTGTGTCATTTTTTTTGAA+TGG | - | 50230:892-911 | MS.gene058522:intron | 25.0% |
| AAATCTGATAAGGTTTCAAG+TGG | - | 50230:390-409 | MS.gene058522:intron | 30.0% | |
| AAGAGTTATCATTTAAGTGG+GGG | - | 50230:1746-1765 | MS.gene058522:CDS | 30.0% | |
| AATATATTCTGGGCAGTATA+TGG | - | 50230:1609-1628 | MS.gene058522:intron | 30.0% | |
| ATTGATATTGTGTGAATCCA+GGG | - | 50230:1091-1110 | MS.gene058522:intron | 30.0% | |
| CAACCAAGGAAATATATTCT+GGG | - | 50230:1599-1618 | MS.gene058522:intron | 30.0% | |
| GGTTATGGAATTGTTTATCA+TGG | - | 50230:588-607 | MS.gene058522:intron | 30.0% | |
| TACGACACAAAAATCTTGTT+AGG | - | 50230:2035-2054 | MS.gene058522:CDS | 30.0% | |
| TGAGAAAGAATTCAAAGTAG+AGG | - | 50230:1994-2013 | MS.gene058522:CDS | 30.0% | |
| TGTGGTTGTTAAATCTGATA+AGG | - | 50230:380-399 | MS.gene058522:intron | 30.0% | |
| TTGAAGACTTAAAGACAACA+AGG | + | 50230:1321-1340 | MS.gene058522:intergenic | 30.0% | |
| ! | TTTTGCTTATGCTTGGAATT+TGG | - | 50230:794-813 | MS.gene058522:intron | 30.0% |
| !! | ACACATACTTGTTATTCAAG+AGG | + | 50230:650-669 | MS.gene058522:intergenic | 30.0% |
| AAAAATCTTGTTAGGCTGCT+TGG | - | 50230:2043-2062 | MS.gene058522:CDS | 35.0% | |
| AAAGTAGAGGTAGAAGCAAT+TGG | - | 50230:2007-2026 | MS.gene058522:CDS | 35.0% | |
| ACTCTTAAGGATGCAAGATA+AGG | + | 50230:1732-1751 | MS.gene058522:intergenic | 35.0% | |
| AGAAGAAGACAATACAAGAC+CGG | + | 50230:1930-1949 | MS.gene058522:intergenic | 35.0% | |
| ATCTCTTGGATTTCTTTAGC+TGG | + | 50230:276-295 | MS.gene058522:intergenic | 35.0% | |
| CATTCCATGGAGCAATTATA+CGG | + | 50230:1366-1385 | MS.gene058522:intergenic | 35.0% | |
| CATTGATATTGTGTGAATCC+AGG | - | 50230:1090-1109 | MS.gene058522:intron | 35.0% | |
| CTTTGAATTCTTTCTCAGCT+TGG | + | 50230:1991-2010 | MS.gene058522:intergenic | 35.0% | |
| GCAACCAAGGAAATATATTC+TGG | - | 50230:1598-1617 | MS.gene058522:intron | 35.0% | |
| TATCATGGTGTTCTTACTGA+TGG | - | 50230:603-622 | MS.gene058522:intron | 35.0% | |
| TGCATTCTCATGTATATTGC+TGG | - | 50230:1839-1858 | MS.gene058522:CDS | 35.0% | |
| TGCTTGGATATTGTGTTGAA+GGG | - | 50230:2059-2078 | MS.gene058522:CDS | 35.0% | |
| TTGTGTCCTGAAAATGTTCT+TGG | - | 50230:558-577 | MS.gene058522:intron | 35.0% | |
| ! | ACAACGTGACGGAATATAAA+TGG | - | 50230:1456-1475 | MS.gene058522:intron | 35.0% |
| ! | GCTTTTTGTTAGCACTTGTT+TGG | - | 50230:1811-1830 | MS.gene058522:CDS | 35.0% |
| ! | GTCTTCAATAATTTGCCTGT+TGG | - | 50230:1333-1352 | MS.gene058522:intron | 35.0% |
| !! | ATTCAACTTGCTGTTAGTGA+AGG | - | 50230:1894-1913 | MS.gene058522:CDS | 35.0% |
| !! | GTTTGAGTTTTGCTTATGCT+TGG | - | 50230:787-806 | MS.gene058522:intron | 35.0% |
| !!! | TTAGCTGCTTTTCCTTGATT+TGG | - | 50230:1045-1064 | MS.gene058522:intron | 35.0% |
| AAGGAAAAGCAGCTAATGCA+AGG | + | 50230:1041-1060 | MS.gene058522:intergenic | 40.0% | |
| CCTGAAAATGTTCTTGGTGA+AGG | - | 50230:564-583 | MS.gene058522:intron | 40.0% | |
| CTGCCCAGAATATATTTCCT+TGG | + | 50230:1605-1624 | MS.gene058522:intergenic | 40.0% | |
| CTGCTTGGATATTGTGTTGA+AGG | - | 50230:2058-2077 | MS.gene058522:CDS | 40.0% | |
| GAAAATGTTCTTGGTGAAGG+TGG | - | 50230:567-586 | MS.gene058522:intron | 40.0% | |
| GAAGAAGACAATACAAGACC+GGG | + | 50230:1929-1948 | MS.gene058522:intergenic | 40.0% | |
| GCTTAAAACACACAACGTGA+CGG | - | 50230:1445-1464 | MS.gene058522:intron | 40.0% | |
| GCTTGGATATTGTGTTGAAG+GGG | - | 50230:2060-2079 | MS.gene058522:CDS | 40.0% | |
| TATTTCCTTGGTTGCAAACG+AGG | + | 50230:1593-1612 | MS.gene058522:intergenic | 40.0% | |
| ! | ATTTTCCTCGTTTGCAACCA+AGG | - | 50230:1585-1604 | MS.gene058522:intron | 40.0% |
| ! | CCTTCACCAAGAACATTTTC+AGG | + | 50230:567-586 | MS.gene058522:intergenic | 40.0% |
| ! | GTTTCAAGTGGTGAGAGTAA+GGG | - | 50230:402-421 | MS.gene058522:intron | 40.0% |
| ! | TGATTTTGTAGTCTCCGGTA+CGG | + | 50230:242-261 | MS.gene058522:intergenic | 40.0% |
| !! | TCTTTTGGAAGTGGTAGTGT+TGG | - | 50230:456-475 | MS.gene058522:intron | 40.0% |
| ACCGGAGACTACAAAATCAC+CGG | - | 50230:243-262 | MS.gene058522:CDS | 45.0% | |
| AGAGAGCTTGAAGATGCTAC+TGG | - | 50230:531-550 | MS.gene058522:intron | 45.0% | |
| CCAACCGTATAATTGCTCCA+TGG | - | 50230:1359-1378 | MS.gene058522:intron | 45.0% | |
| CCATGGAGCAATTATACGGT+TGG | + | 50230:1362-1381 | MS.gene058522:intergenic | 45.0% | |
| GATATTGTGTGAATCCAGGG+AGG | - | 50230:1094-1113 | MS.gene058522:intron | 45.0% | |
| GTTCTTGGTGAAGGTGGTTA+TGG | - | 50230:573-592 | MS.gene058522:intron | 45.0% | |
| TGCTGTTAGTGAAGGTTCCT+TGG | - | 50230:1902-1921 | MS.gene058522:CDS | 45.0% | |
| TTCATTTGTTTCGTCCTCCC+TGG | + | 50230:1111-1130 | MS.gene058522:intergenic | 45.0% | |
| ! | GGTTTCAAGTGGTGAGAGTA+AGG | - | 50230:401-420 | MS.gene058522:intron | 45.0% |
| ! | GTCCTGAAGTGTCACATCTT+GGG | - | 50230:487-506 | MS.gene058522:intron | 45.0% |
| ! | TCCGGTGATTTTGTAGTCTC+CGG | + | 50230:247-266 | MS.gene058522:intergenic | 45.0% |
| ! | TGAAGTGTCACATCTTGGGT+GGG | - | 50230:491-510 | MS.gene058522:intron | 45.0% |
| ! | TGTGAAACGACGTCGTCTTT+TGG | - | 50230:441-460 | MS.gene058522:intron | 45.0% |
| ! | TTATACGGTTGGTTGCCAAC+AGG | + | 50230:1351-1370 | MS.gene058522:intergenic | 45.0% |
| ! | TTTGGAAGTGGTAGTGTTGG+TGG | - | 50230:459-478 | MS.gene058522:intron | 45.0% |
| !! | TTGGAAGTGGTAGTGTTGGT+GGG | - | 50230:460-479 | MS.gene058522:intron | 45.0% |
| ACAATACAAGACCGGGACCA+AGG | + | 50230:1922-1941 | MS.gene058522:intergenic | 50.0% | |
| AGTAAGGGAACAGTGGGAAG+CGG | - | 50230:417-436 | MS.gene058522:intron | 50.0% | |
| CAAGAGATCGTGCATGTTCC+AGG | - | 50230:288-307 | MS.gene058522:CDS | 50.0% | |
| CACCCAAGATGTGACACTTC+AGG | + | 50230:492-511 | MS.gene058522:intergenic | 50.0% | |
| CTGGAACATGCACGATCTCT+TGG | + | 50230:290-309 | MS.gene058522:intergenic | 50.0% | |
| CTTGTCTTCCAATGTCCACG+TGG | + | 50230:353-372 | MS.gene058522:intergenic | 50.0% | |
| GAGCTTGAAGATGCTACTGG+TGG | - | 50230:534-553 | MS.gene058522:intron | 50.0% | |
| GGTGAGAGTAAGGGAACAGT+GGG | - | 50230:411-430 | MS.gene058522:intron | 50.0% | |
| TAGTGAAGGTTCCTTGGTCC+CGG | - | 50230:1908-1927 | MS.gene058522:CDS | 50.0% | |
| TGGTGAGAGTAAGGGAACAG+TGG | - | 50230:410-429 | MS.gene058522:intron | 50.0% | |
| TTCTGGGCAGTATATGGAGC+AGG | - | 50230:1615-1634 | MS.gene058522:intron | 50.0% | |
| TTCTTTAGCTGGTGCAGCTC+CGG | + | 50230:265-284 | MS.gene058522:intergenic | 50.0% | |
| ! | CTGAAGTGTCACATCTTGGG+TGG | - | 50230:490-509 | MS.gene058522:intron | 50.0% |
| ! | GAAGTGTCACATCTTGGGTG+GGG | - | 50230:492-511 | MS.gene058522:intron | 50.0% |
| ! | GGTCCTGAAGTGTCACATCT+TGG | - | 50230:486-505 | MS.gene058522:intron | 50.0% |
| ! | TTTTGTAGTCTCCGGTACGG+CGG | + | 50230:239-258 | MS.gene058522:intergenic | 50.0% |
| !! | ACGACGTCGTCTTTTGGAAG+TGG | - | 50230:447-466 | MS.gene058522:intron | 50.0% |
| !! | AGTGGTAGTGTTGGTGGGAT+TGG | - | 50230:465-484 | MS.gene058522:intron | 50.0% |
| !! | TGGAAGACAAGAGCATCGTG+TGG | - | 50230:362-381 | MS.gene058522:intron | 50.0% |
| CACATCTTGGGTGGGGTAGA+TGG | - | 50230:499-518 | MS.gene058522:intron | 55.0% | |
| CCAATGTCCACGTGGATCTC+CGG | + | 50230:345-364 | MS.gene058522:intergenic | 55.0% | |
| GTAAGGGAACAGTGGGAAGC+GGG | - | 50230:418-437 | MS.gene058522:intron | 55.0% | |
| GTTGGTCGTCGGAGCATATG+TGG | + | 50230:315-334 | MS.gene058522:intergenic | 55.0% | |
| TATGCTCCGACGACCAACTC+CGG | - | 50230:317-336 | MS.gene058522:intron | 55.0% | |
| CCGGAGATCCACGTGGACAT+TGG | - | 50230:342-361 | MS.gene058522:intron | 60.0% | |
| CGTCGGAGCATATGTGGACC+TGG | + | 50230:309-328 | MS.gene058522:intergenic | 60.0% | |
| TCCACGTGGATCTCCGGTAC+CGG | + | 50230:339-358 | MS.gene058522:intergenic | 60.0% | |
| ! | TGGATCTCCGGTACCGGAGT+TGG | + | 50230:333-352 | MS.gene058522:intergenic | 60.0% |
| AGTCTCCGGTACGGCGGTGA+CGG | + | 50230:233-252 | MS.gene058522:intergenic | 65.0% | |
| CCGACGACCAACTCCGGTAC+CGG | - | 50230:323-342 | MS.gene058522:intron | 65.0% | |
| ! | CCGGTACCGGAGTTGGTCGT+CGG | + | 50230:326-345 | MS.gene058522:intergenic | 65.0% |
| ! | TCCGGTACCGGAGATCCACG+TGG | - | 50230:335-354 | MS.gene058522:intron | 65.0% |
| CGTCGCCGTCACCGCCGTAC+CGG | - | 50230:225-244 | MS.gene058522:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50230 | gene | 220 | 2089 | 220 | ID=MS.gene058522 |
| 50230 | mRNA | 220 | 2089 | 220 | ID=MS.gene058522.t1;Parent=MS.gene058522 |
| 50230 | exon | 1648 | 2089 | 1648 | ID=MS.gene058522.t1.exon1;Parent=MS.gene058522.t1 |
| 50230 | CDS | 1648 | 2089 | 1648 | ID=cds.MS.gene058522.t1;Parent=MS.gene058522.t1 |
| 50230 | exon | 220 | 324 | 220 | ID=MS.gene058522.t1.exon2;Parent=MS.gene058522.t1 |
| 50230 | CDS | 220 | 324 | 220 | ID=cds.MS.gene058522.t1;Parent=MS.gene058522.t1 |
| Gene Sequence |
| Protein sequence |
