AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058643


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058643.t1 MTR_8g030510 69.355 186 57 0 1 186 1 186 4.22e-92 267
MS.gene058643.t1 MTR_8g098665 39.216 204 104 4 1 186 1 202 5.93e-40 135
MS.gene058643.t1 MTR_6g015980 35.754 179 91 3 28 186 49 223 1.14e-27 104
MS.gene058643.t1 MTR_7g016840 35.500 200 93 6 13 186 34 223 6.54e-26 99.8
MS.gene058643.t1 MTR_5g046970 40.000 95 52 2 93 182 32 126 1.38e-14 68.2
MS.gene058643.t1 MTR_2g087820 54.167 48 22 0 137 184 34 81 9.33e-13 65.9
MS.gene058643.t1 MTR_2g087820 54.167 48 22 0 137 184 34 81 9.96e-13 65.9
MS.gene058643.t1 MTR_7g106770 54.348 46 21 0 137 182 31 76 7.28e-12 62.8
MS.gene058643.t1 MTR_2g022680 52.083 48 23 0 137 184 35 82 2.11e-11 62.0
MS.gene058643.t1 MTR_2g022680 52.083 48 23 0 137 184 35 82 2.38e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058643.t1 AT4G00335 57.527 186 78 1 1 186 1 185 4.42e-73 219
MS.gene058643.t1 AT4G00335 57.527 186 78 1 1 186 1 185 4.42e-73 219
MS.gene058643.t1 AT4G00335 57.527 186 78 1 1 186 1 185 4.42e-73 219
MS.gene058643.t1 AT5G41350 41.063 207 101 6 1 186 1 207 5.75e-43 143
MS.gene058643.t1 AT5G41350 41.063 207 101 6 1 186 1 207 5.75e-43 143
MS.gene058643.t1 AT5G41350 41.063 207 101 6 1 186 26 232 1.01e-42 143
MS.gene058643.t1 AT5G38895 37.333 150 76 5 52 186 69 215 1.06e-22 90.9
MS.gene058643.t1 AT3G02290 35.583 163 65 5 56 186 73 227 1.12e-22 91.3
MS.gene058643.t1 AT3G02290 35.583 163 65 5 56 186 73 227 1.12e-22 91.3
MS.gene058643.t1 AT3G02290 35.583 163 65 5 56 186 73 227 1.12e-22 91.3
MS.gene058643.t1 AT3G02290 35.583 163 65 5 56 186 73 227 1.12e-22 91.3
MS.gene058643.t1 AT3G02290 35.583 163 65 5 56 186 73 227 1.12e-22 91.3
MS.gene058643.t1 AT5G38895 37.333 150 76 5 52 186 144 290 3.33e-22 91.3
MS.gene058643.t1 AT5G38895 37.333 150 76 5 52 186 183 329 5.62e-22 91.3
MS.gene058643.t1 AT3G02290 34.969 163 66 5 56 186 73 227 6.05e-22 89.4
MS.gene058643.t1 AT5G15790 33.511 188 89 3 27 186 49 228 1.08e-21 88.6
MS.gene058643.t1 AT5G15790 33.511 188 89 3 27 186 49 228 1.08e-21 88.6
MS.gene058643.t1 AT5G15790 33.511 188 89 3 27 186 49 228 1.08e-21 88.6
MS.gene058643.t1 AT5G15790 33.511 188 89 3 27 186 49 228 1.08e-21 88.6
MS.gene058643.t1 AT5G15790 32.979 188 90 3 27 186 49 228 9.14e-21 87.0
MS.gene058643.t1 AT4G23450 67.308 52 17 0 135 186 99 150 2.26e-20 83.2
MS.gene058643.t1 AT4G23450 67.308 52 17 0 135 186 99 150 2.26e-20 83.2
MS.gene058643.t1 AT4G23450 67.308 52 17 0 135 186 99 150 2.26e-20 83.2
MS.gene058643.t1 AT4G23450 67.308 52 17 0 135 186 99 150 2.26e-20 83.2
MS.gene058643.t1 AT4G23450 67.308 52 17 0 135 186 154 205 3.75e-20 84.0
MS.gene058643.t1 AT4G23450 67.308 52 17 0 135 186 154 205 3.75e-20 84.0
MS.gene058643.t1 AT1G69085 44.737 76 40 1 113 186 31 106 3.54e-17 73.9
MS.gene058643.t1 AT5G22000 50.000 48 24 0 137 184 30 77 3.49e-11 61.2
MS.gene058643.t1 AT5G22000 50.000 48 24 0 137 184 30 77 3.62e-11 61.2
MS.gene058643.t1 AT5G22000 50.000 48 24 0 137 184 30 77 3.62e-11 61.2
MS.gene058643.t1 AT4G14220 46.000 50 27 0 137 186 43 92 4.32e-11 61.2

Find 40 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGATCTCTGCTGGATTTC+TGG 0.071724 7.4:+28207748 MS.gene058643:CDS
ACTGTACCTTCGAGGCAAAT+AGG 0.360597 7.4:-28208049 None:intergenic
TCTTTGCAAGACTCGGAATC+AGG 0.371530 7.4:-28207863 None:intergenic
CGAAGCAGTGGTAGCGTCAT+TGG 0.377536 7.4:-28207729 None:intergenic
TTTGATAGTTCTACCTTCCT+TGG 0.379960 7.4:-28207980 None:intergenic
GGAGGAGACCGGAATGTATC+AGG 0.380787 7.4:-28207797 None:intergenic
CTACTAACATAGTAATAAAC+TGG 0.386112 7.4:-28207181 None:intergenic
CTTCCTTATGACACTGTCTT+CGG 0.402519 7.4:+28207827 MS.gene058643:CDS
ATCTCTGCTGGATTTCTGGT+TGG 0.413962 7.4:+28207752 MS.gene058643:CDS
TATCAGGTATGGATGCTTCC+AGG 0.417514 7.4:-28207781 None:intergenic
CTGTACCTTCGAGGCAAATA+GGG 0.426908 7.4:-28208048 None:intergenic
ATTTCTGGTTGGCTTGAACC+TGG 0.430575 7.4:+28207763 MS.gene058643:CDS
AATATCATCGTGTGTTATTG+AGG 0.438311 7.4:-28207912 None:intergenic
ACCACTGCTTCGATCTCTGC+TGG 0.451923 7.4:+28207740 MS.gene058643:CDS
ACCTATCTTGCATTCTTGAG+TGG 0.468479 7.4:+28208259 MS.gene058643:CDS
TCCACTCAAGAATGCAAGAT+AGG 0.479262 7.4:-28208260 None:intergenic
GACTCCCGCTCTTCAAAAGT+TGG 0.485903 7.4:-28207698 None:intergenic
ATCTTGCATTCTTGAGTGGA+TGG 0.491376 7.4:+28208263 MS.gene058643:CDS
GTAGAACTATCAAAATCAAA+TGG 0.495198 7.4:+28207989 MS.gene058643:CDS
GTGTCATAAGGAAGAGGCAC+AGG 0.502780 7.4:-28207818 None:intergenic
CATCCATACCTGATACATTC+CGG 0.503588 7.4:+28207789 MS.gene058643:CDS
GGACATACCTGGTCACATAT+AGG 0.506264 7.4:-28208303 None:intergenic
CACAAGCAACTGTACCTTCG+AGG 0.509741 7.4:-28208057 None:intergenic
CCCGCTCTTCAAAAGTTGGC+GGG 0.514822 7.4:-28207694 None:intergenic
CCTCCGAAGACAGTGTCATA+AGG 0.517025 7.4:-28207830 None:intergenic
TCCCGCTCTTCAAAAGTTGG+CGG 0.524615 7.4:-28207695 None:intergenic
TGCTGCCCTATTTGCCTCGA+AGG 0.526587 7.4:+28208043 MS.gene058643:CDS
AGACCGGAATGTATCAGGTA+TGG 0.527716 7.4:-28207792 None:intergenic
AGTATTTGCAACAGAAGAAG+AGG 0.529174 7.4:+28208018 MS.gene058643:CDS
CTCTTGTCCTATATGTGACC+AGG 0.534143 7.4:+28208296 MS.gene058643:CDS
GAAGAGGCACAGGAGGAGAC+CGG 0.549690 7.4:-28207808 None:intergenic
TCCAGCAGAGATCGAAGCAG+TGG 0.566172 7.4:-28207741 None:intergenic
GCCAGAAAACATCATTTGCA+AGG 0.567191 7.4:+28207154 MS.gene058643:CDS
TGCATCTTTATCTCCAAGGA+AGG 0.579543 7.4:+28207967 MS.gene058643:CDS
CCTTATGACACTGTCTTCGG+AGG 0.580401 7.4:+28207830 MS.gene058643:CDS
AATCTGCATCTTTATCTCCA+AGG 0.582568 7.4:+28207963 MS.gene058643:CDS
TCATAAGGAAGAGGCACAGG+AGG 0.585245 7.4:-28207815 None:intergenic
AAGACAGTGTCATAAGGAAG+AGG 0.585295 7.4:-28207824 None:intergenic
AGCAGTGGTAGCGTCATTGG+AGG 0.588345 7.4:-28207726 None:intergenic
AAGAACTTCTGGAAAATGGG+AGG 0.645337 7.4:+28207118 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTAGATTATAGTTTA+AGG + chr7.4:28207393-28207412 MS.gene058643:intron 15.0%
!! ATAGTTTAAGGATTATTGTT+TGG + chr7.4:28207405-28207424 MS.gene058643:intron 20.0%
!! TCACATAAATACTTATGTAT+AGG - chr7.4:28207574-28207593 None:intergenic 20.0%
!!! AAATGTCGATTTTTATCTTA+TGG + chr7.4:28208335-28208354 MS.gene058643:intron 20.0%
!!! AATATTTTCATAAGTTGACT+TGG - chr7.4:28207496-28207515 None:intergenic 20.0%
!!! TTTTTTTCATTTGATGTTAC+AGG + chr7.4:28208562-28208581 MS.gene058643:intron 20.0%
! AAAAATCGACATTTAAGAAC+AGG - chr7.4:28208329-28208348 None:intergenic 25.0%
! AAAATCGACATTTAAGAACA+GGG - chr7.4:28208328-28208347 None:intergenic 25.0%
! AATGTTGGAGATGAAAATAA+TGG - chr7.4:28207249-28207268 None:intergenic 25.0%
! CTACTAACATAGTAATAAAC+TGG - chr7.4:28207184-28207203 None:intergenic 25.0%
! GTAGAACTATCAAAATCAAA+TGG + chr7.4:28207989-28208008 MS.gene058643:CDS 25.0%
!! TCACATTTTGTCAGATTTTT+TGG - chr7.4:28208228-28208247 None:intergenic 25.0%
!! TTATTGTTTGGTAGAACTTT+TGG + chr7.4:28207417-28207436 MS.gene058643:intron 25.0%
!!! ATAGGCTATTTCTATAACAA+AGG - chr7.4:28207556-28207575 None:intergenic 25.0%
AAATCGACATTTAAGAACAG+GGG - chr7.4:28208327-28208346 None:intergenic 30.0%
AATAAAAAGGTGACGAATGT+TGG - chr7.4:28207264-28207283 None:intergenic 30.0%
TGCTTGTGTAACTTAAAGAT+GGG + chr7.4:28208072-28208091 MS.gene058643:intron 30.0%
TTGCTTGTGTAACTTAAAGA+TGG + chr7.4:28208071-28208090 MS.gene058643:intron 30.0%
! AATATCATCGTGTGTTATTG+AGG - chr7.4:28207915-28207934 None:intergenic 30.0%
! GTACTTCTGAAAGAATAATC+TGG + chr7.4:28207354-28207373 MS.gene058643:intron 30.0%
! GTTTTCTCAAACTATTACCA+AGG + chr7.4:28208440-28208459 MS.gene058643:intron 30.0%
AAGAGAACAGCGAAAACAAA+TGG - chr7.4:28207325-28207344 None:intergenic 35.0%
AATCTGCATCTTTATCTCCA+AGG + chr7.4:28207963-28207982 MS.gene058643:CDS 35.0%
AGTATTTGCAACAGAAGAAG+AGG + chr7.4:28208018-28208037 MS.gene058643:CDS 35.0%
ATGTTTCAGCCTATTACACA+AGG + chr7.4:28208115-28208134 MS.gene058643:intron 35.0%
CTTGGAGAGCTAATAGAAAT+AGG - chr7.4:28207478-28207497 None:intergenic 35.0%
TCTGAAAGAATAATCTGGTC+TGG + chr7.4:28207359-28207378 MS.gene058643:intron 35.0%
! ACACGTCTAGAAGATGTTTT+CGG + chr7.4:28207452-28207471 MS.gene058643:intron 35.0%
! GACATTTTCTTTGCAAGACT+CGG - chr7.4:28207873-28207892 None:intergenic 35.0%
! TCCTTGCAAATGATGTTTTC+TGG - chr7.4:28207158-28207177 None:intergenic 35.0%
! TTTGATAGTTCTACCTTCCT+TGG - chr7.4:28207983-28208002 None:intergenic 35.0%
AAGACAGTGTCATAAGGAAG+AGG - chr7.4:28207827-28207846 None:intergenic 40.0%
ACCTATCTTGCATTCTTGAG+TGG + chr7.4:28208259-28208278 MS.gene058643:CDS 40.0%
ATCTTGCATTCTTGAGTGGA+TGG + chr7.4:28208263-28208282 MS.gene058643:CDS 40.0%
CATCCATACCTGATACATTC+CGG + chr7.4:28207789-28207808 MS.gene058643:CDS 40.0%
CTTACTCCTCCTTGTGTAAT+AGG - chr7.4:28208127-28208146 None:intergenic 40.0%
CTTCCTTATGACACTGTCTT+CGG + chr7.4:28207827-28207846 MS.gene058643:CDS 40.0%
GATTGAATGTAACCACGTGT+TGG - chr7.4:28208415-28208434 None:intergenic 40.0%
GCATTAGCAATGATTGTGTG+AGG + chr7.4:28207297-28207316 MS.gene058643:intron 40.0%
GCCAGAAAACATCATTTGCA+AGG + chr7.4:28207154-28207173 MS.gene058643:CDS 40.0%
TCCACTCAAGAATGCAAGAT+AGG - chr7.4:28208263-28208282 None:intergenic 40.0%
TGCATCTTTATCTCCAAGGA+AGG + chr7.4:28207967-28207986 MS.gene058643:CDS 40.0%
TTTCAGCCTATTACACAAGG+AGG + chr7.4:28208118-28208137 MS.gene058643:intron 40.0%
ACTGTACCTTCGAGGCAAAT+AGG - chr7.4:28208052-28208071 None:intergenic 45.0%
AGACCGGAATGTATCAGGTA+TGG - chr7.4:28207795-28207814 None:intergenic 45.0%
AGCACCACACTTCAGATTGA+AGG + chr7.4:28208358-28208377 MS.gene058643:intron 45.0%
ATCGATACGAAACCAACACG+TGG + chr7.4:28208400-28208419 MS.gene058643:intron 45.0%
CTCTTGTCCTATATGTGACC+AGG + chr7.4:28208296-28208315 MS.gene058643:CDS 45.0%
CTGTACCTTCGAGGCAAATA+GGG - chr7.4:28208051-28208070 None:intergenic 45.0%
GGACATACCTGGTCACATAT+AGG - chr7.4:28208306-28208325 None:intergenic 45.0%
TATCAGGTATGGATGCTTCC+AGG - chr7.4:28207784-28207803 None:intergenic 45.0%
TCTTTGCAAGACTCGGAATC+AGG - chr7.4:28207866-28207885 None:intergenic 45.0%
TTAAGAACAGGGGACATACC+TGG - chr7.4:28208317-28208336 None:intergenic 45.0%
TTCGATCTCTGCTGGATTTC+TGG + chr7.4:28207748-28207767 MS.gene058643:CDS 45.0%
! AAGTTGGCGGGCACTAAAAA+CGG - chr7.4:28207685-28207704 None:intergenic 45.0%
! CGTATCGATGTCAAACACAC+CGG - chr7.4:28208389-28208408 None:intergenic 45.0%
! GACACCTTCAATCTGAAGTG+TGG - chr7.4:28208365-28208384 None:intergenic 45.0%
!! ATCTCTGCTGGATTTCTGGT+TGG + chr7.4:28207752-28207771 MS.gene058643:CDS 45.0%
!! ATTTCTGGTTGGCTTGAACC+TGG + chr7.4:28207763-28207782 MS.gene058643:CDS 45.0%
!! CTTCAGATTGAAGGTGTCAC+CGG + chr7.4:28208367-28208386 MS.gene058643:intron 45.0%
!! TAATGATTAAAATAATAAAA+AGG - chr7.4:28207277-28207296 None:intergenic 5.0%
CACAAGCAACTGTACCTTCG+AGG - chr7.4:28208060-28208079 None:intergenic 50.0%
CCTCCGAAGACAGTGTCATA+AGG - chr7.4:28207833-28207852 None:intergenic 50.0%
CCTTATGACACTGTCTTCGG+AGG + chr7.4:28207830-28207849 MS.gene058643:CDS 50.0%
GACTCCCGCTCTTCAAAAGT+TGG - chr7.4:28207701-28207720 None:intergenic 50.0%
TCCCGCTCTTCAAAAGTTGG+CGG - chr7.4:28207698-28207717 None:intergenic 50.0%
TCGACGTGTTAGTGTCGTGT+CGG + chr7.4:28208463-28208482 MS.gene058643:intron 50.0%
! GTGTCATAAGGAAGAGGCAC+AGG - chr7.4:28207821-28207840 None:intergenic 50.0%
! TCATAAGGAAGAGGCACAGG+AGG - chr7.4:28207818-28207837 None:intergenic 50.0%
!! GTTAGTGTCGTGTCGGTTGT+TGG + chr7.4:28208470-28208489 MS.gene058643:intron 50.0%
ACCACTGCTTCGATCTCTGC+TGG + chr7.4:28207740-28207759 MS.gene058643:CDS 55.0%
CCCGCTCTTCAAAAGTTGGC+GGG - chr7.4:28207697-28207716 None:intergenic 55.0%
CGACACTAACACGTCGACCT+TGG - chr7.4:28208460-28208479 None:intergenic 55.0%
GGAGGAGACCGGAATGTATC+AGG - chr7.4:28207800-28207819 None:intergenic 55.0%
TCCAGCAGAGATCGAAGCAG+TGG - chr7.4:28207744-28207763 None:intergenic 55.0%
TGCTGCCCTATTTGCCTCGA+AGG + chr7.4:28208043-28208062 MS.gene058643:CDS 55.0%
! AGCAGTGGTAGCGTCATTGG+AGG - chr7.4:28207729-28207748 None:intergenic 55.0%
! CGAAGCAGTGGTAGCGTCAT+TGG - chr7.4:28207732-28207751 None:intergenic 55.0%
!! CCCGCCAACTTTTGAAGAGC+GGG + chr7.4:28207694-28207713 MS.gene058643:CDS 55.0%
!! GCCCGCCAACTTTTGAAGAG+CGG + chr7.4:28207693-28207712 MS.gene058643:CDS 55.0%
! GAAGAGGCACAGGAGGAGAC+CGG - chr7.4:28207811-28207830 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 28207133 28208598 28207133 ID=MS.gene058643
chr7.4 mRNA 28207133 28208598 28207133 ID=MS.gene058643.t1;Parent=MS.gene058643
chr7.4 exon 28207133 28207195 28207133 ID=MS.gene058643.t1.exon1;Parent=MS.gene058643.t1
chr7.4 CDS 28207133 28207195 28207133 ID=cds.MS.gene058643.t1;Parent=MS.gene058643.t1
chr7.4 exon 28207692 28208064 28207692 ID=MS.gene058643.t1.exon2;Parent=MS.gene058643.t1
chr7.4 CDS 28207692 28208064 28207692 ID=cds.MS.gene058643.t1;Parent=MS.gene058643.t1
chr7.4 exon 28208208 28208317 28208208 ID=MS.gene058643.t1.exon3;Parent=MS.gene058643.t1
chr7.4 CDS 28208208 28208317 28208208 ID=cds.MS.gene058643.t1;Parent=MS.gene058643.t1
chr7.4 exon 28208584 28208598 28208584 ID=MS.gene058643.t1.exon4;Parent=MS.gene058643.t1
chr7.4 CDS 28208584 28208598 28208584 ID=cds.MS.gene058643.t1;Parent=MS.gene058643.t1
Gene Sequence

>MS.gene058643

ATGGGAGGTTGCTGTTGTTCTGCCAGAAAACATCATTTGCAAGGAACACCAGTTTATTACTATGTTAGTAGATTTCTATCTTCTCTTTTAACAATATATCTAGTAATCTCGCTCCATTATTTTCATCTCCAACATTCGTCACCTTTTTATTATTTTAATCATTAGCATTAGCAATGATTGTGTGAGGAACCATTTGTTTTCGCTGTTCTCTTCAAACTTAAGTACTTCTGAAAGAATAATCTGGTCTGGCTACAAGTTTATTGTTTTAGATTATAGTTTAAGGATTATTGTTTGGTAGAACTTTTGGAAACAGCTATTGACACGTCTAGAAGATGTTTTCGGCCTATTTCTATTAGCTCTCCAAGTCAACTTATGAAAATATTTTATAGCTTATATGAAAACAATTTGACTTTATTTTATCCTTTGTTATAGAAATAGCCTATACATAAGTATTTATGTGATAAGTGTTTCTGCTATAAGCATAAAAGTGCTTAACTAAACTGTTTATTTATATTTTGTTATGTGTGTATGATTTATAACATGAAAACTCCGTTTTTAGTGCCCGCCAACTTTTGAAGAGCGGGAGTCTTTAACCTCCAATGACGCTACCACTGCTTCGATCTCTGCTGGATTTCTGGTTGGCTTGAACCTGGAAGCATCCATACCTGATACATTCCGGTCTCCTCCTGTGCCTCTTCCTTATGACACTGTCTTCGGAGGTTCTGCATCACCTGATTCCGAGTCTTGCAAAGAAAATGTCAGTTTTAGTAGCTTCGAAACCTCAATAACACACGATGATATTGAAGAATCAGATTGTAAAACTCTACAAAAATCTGCATCTTTATCTCCAAGGAAGGTAGAACTATCAAAATCAAATGGAACTCAAGTATTTGCAACAGAAGAAGAGGATTGCTGCCCTATTTGCCTCGAAGGTACAGTTGCTTGTGTAACTTAAAGATGGGTATTACGTGTTAGATTTTTTATGTTTCAGCCTATTACACAAGGAGGAGTAAGCTATTTTTTTCTCTTAGTATGTTAAAAGCAATCATTTGAGATTTTTTCATTTTTAATGCAGAATATGATGACGATAATCCAAAAAATCTGACAAAATGTGAACATCATTTTCACCTATCTTGCATTCTTGAGTGGATGGAAAGAAGTGACTCTTGTCCTATATGTGACCAGGTATGTCCCCTGTTCTTAAATGTCGATTTTTATCTTATGGAGCACCACACTTCAGATTGAAGGTGTCACCGGTGTGTTTGACATCGATACGAAACCAACACGTGGTTACATTCAATCATTTTGTTTTCTCAAACTATTACCAAGGTCGACGTGTTAGTGTCGTGTCGGTTGTTGGTGTCTGTGTTAGTGCTTCATAGTTTTCATATTATAAATTTATTATGTGCTTTAGTGAAATGTTTATTTTTTTTTTTCATTTGATGTTACAGGAGATGATATTTTGA

Protein sequence

>MS.gene058643.t1

MGGCCCSARKHHLQGTPVYYYCPPTFEERESLTSNDATTASISAGFLVGLNLEASIPDTFRSPPVPLPYDTVFGGSASPDSESCKENVSFSSFETSITHDDIEESDCKTLQKSASLSPRKVELSKSNGTQVFATEEEDCCPICLEEYDDDNPKNLTKCEHHFHLSCILEWMERSDSCPICDQEMIF