AlfalfaGEDB | MS.gene058677

M. sativa cultivar XinJiangDaYe / MS.gene058677

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058677.t1	RHN47048.1	86.4	162	22	0	1	162	1	162	7.90E-71	276.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058677.t1	Q9FFS3	63.2	57	13	1	105	161	70	118	3.2e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058677.t1	G7L6N9	87.0	161	21	0	1	161	1	161	5.7e-71	276.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene058677.t1	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058677	MS.gene061777	0.800505	1.40E-48	-1.69E-46
MS.gene058677	MS.gene09819	0.81245	4.21E-51	-1.69E-46
MS.gene058677	MS.gene22491	0.800001	1.77E-48	-1.69E-46
MS.gene058677	MS.gene44966	0.821639	3.63E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058677.t1	MTR_7g079010	95.652	161	7	0	1	161	1	161	2.42e-113	321
MS.gene058677.t1	MTR_8g007270	65.116	43	15	0	119	161	132	174	1.42e-13	66.2
MS.gene058677.t1	MTR_7g028415	84.848	33	5	0	129	161	81	113	1.69e-13	65.1
MS.gene058677.t1	MTR_7g028710	84.848	33	5	0	129	161	81	113	1.69e-13	65.1
MS.gene058677.t1	MTR_3g095080	68.421	38	12	0	127	164	154	191	2.16e-13	65.1
MS.gene058677.t1	MTR_5g029850	69.231	39	12	0	119	157	29	67	1.36e-12	60.5
MS.gene058677.t1	MTR_5g043880	58.929	56	14	1	106	161	98	144	1.82e-12	63.9
MS.gene058677.t1	MTR_4g094588	80.000	35	7	0	127	161	164	198	1.96e-12	64.3
MS.gene058677.t1	MTR_2g033820	80.000	35	7	0	127	161	189	223	2.00e-12	64.3
MS.gene058677.t1	MTR_1g086790	70.000	40	12	0	122	161	139	178	4.03e-12	63.2
MS.gene058677.t1	MTR_5g091390	80.000	35	7	0	127	161	143	177	6.05e-12	62.8
MS.gene058677.t1	MTR_8g092140	69.444	36	10	1	127	161	145	180	1.76e-11	60.8
MS.gene058677.t1	MTR_7g109800	50.000	62	22	1	100	161	113	165	1.92e-11	61.2
MS.gene058677.t1	MTR_3g095040	58.000	50	20	1	113	161	89	138	2.34e-11	60.1
MS.gene058677.t1	MTR_3g095080	68.571	35	11	0	127	161	154	188	2.40e-11	60.5
MS.gene058677.t1	MTR_3g095080	68.571	35	11	0	127	161	130	164	2.80e-11	60.1
MS.gene058677.t1	MTR_8g096730	48.387	62	29	1	100	161	32	90	3.91e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058677.t1	AT5G41570	63.158	57	13	1	105	161	70	118	3.58e-17	75.1
MS.gene058677.t1	AT2G46130	69.565	46	14	0	122	167	11	56	3.94e-17	72.8
MS.gene058677.t1	AT2G46130	75.000	40	10	0	122	161	11	50	6.63e-15	67.4
MS.gene058677.t1	AT1G64000	65.909	44	15	0	118	161	91	134	7.18e-15	69.3
MS.gene058677.t1	AT5G13080	85.294	34	5	0	128	161	54	87	1.55e-14	67.4
MS.gene058677.t1	AT2G03340	38.947	95	54	1	71	161	341	435	2.16e-13	67.4
MS.gene058677.t1	AT1G69310	44.186	86	42	2	82	167	94	173	2.18e-13	66.6
MS.gene058677.t1	AT2G44745	60.465	43	17	0	119	161	123	165	4.12e-13	65.1
MS.gene058677.t1	AT3G01970	82.353	34	6	0	128	161	52	85	4.46e-13	63.5
MS.gene058677.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.36e-13	65.1
MS.gene058677.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.36e-13	65.1
MS.gene058677.t1	AT1G69310	46.250	80	37	2	82	161	94	167	7.36e-13	65.1
MS.gene058677.t1	AT4G39410	52.083	48	23	0	114	161	196	243	2.25e-12	63.9
MS.gene058677.t1	AT5G46350	34.899	149	71	4	28	161	66	203	8.82e-12	62.4
MS.gene058677.t1	AT5G49520	82.353	34	6	0	128	161	208	241	1.18e-11	62.4
MS.gene058677.t1	AT4G18170	62.500	48	14	1	118	161	145	192	1.35e-11	61.6
MS.gene058677.t1	AT2G38470	50.746	67	25	1	103	161	316	382	9.26e-11	59.7

Find 44 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCATCTTCTTCTAATT+TGG	0.109939	7.4:-26760283	MS.gene058677:CDS
AAGAAGTGTTGATGATATTT+TGG	0.221201	7.4:-26760130	MS.gene058677:CDS
ACAACAATAACTTGCTTGTT+GGG	0.273574	7.4:-26760347	MS.gene058677:CDS
ATTAATGGTGGTGGTGGGTT+TGG	0.274937	7.4:+26760510	None:intergenic
GGTGATGGTATATTCATTAA+TGG	0.285795	7.4:+26760495	None:intergenic
GTGGTGGGTTTGGTGGGTCT+TGG	0.304953	7.4:+26760520	None:intergenic
GGTTATCGTTGGAGGAAATA+TGG	0.317694	7.4:-26760102	MS.gene058677:CDS
AACAACAATAACTTGCTTGT+TGG	0.348151	7.4:-26760348	MS.gene058677:CDS
GAAACAAGTTAGAGGAGAAA+AGG	0.356863	7.4:-26760209	MS.gene058677:CDS
TTAGAGGAGAAAAGGGTTAG+AGG	0.362096	7.4:-26760201	MS.gene058677:CDS
AGAAAAGGGAAACAAGTTAG+AGG	0.399768	7.4:-26760217	MS.gene058677:CDS
TTCTTTACAAAACTACCCTT+TGG	0.400016	7.4:-26760427	MS.gene058677:CDS
TCAACTACATGAAGAAGAAA+AGG	0.419090	7.4:-26760232	MS.gene058677:CDS
AAACAAGTTAGAGGAGAAAA+GGG	0.432866	7.4:-26760208	MS.gene058677:CDS
ATATTCATTAATGGTGGTGG+TGG	0.437732	7.4:+26760504	None:intergenic
TAAAGAAGGTGTAAAGAAGA+AGG	0.451312	7.4:+26760443	None:intergenic
GAATCTCAAGATCAAGGCCT+TGG	0.455635	7.4:-26760405	MS.gene058677:CDS
ATGGTGGTGGTGGGTTTGGT+GGG	0.457794	7.4:+26760514	None:intergenic
TGGATGATGGTTATCGTTGG+AGG	0.458848	7.4:-26760110	MS.gene058677:CDS
AATGGTGGTGGTGGGTTTGG+TGG	0.469202	7.4:+26760513	None:intergenic
GTAATTCAAAATAATGAAAG+TGG	0.470864	7.4:-26760261	MS.gene058677:CDS
AAAGAAGGTGTAAAGAAGAA+GGG	0.479028	7.4:+26760444	None:intergenic
AGGAGAAAAGGGTTAGAGGT+GGG	0.487742	7.4:-26760197	MS.gene058677:CDS
CTTGCTTGTTGGGGATGCTA+AGG	0.508570	7.4:-26760337	MS.gene058677:CDS
ACATACATGTAGTATATACC+TGG	0.511478	7.4:+26760050	None:intergenic
CAACTACATGAAGAAGAAAA+GGG	0.514940	7.4:-26760231	MS.gene058677:CDS
TATTCATTAATGGTGGTGGT+GGG	0.530475	7.4:+26760505	None:intergenic
GAGGAGAAAAGGGTTAGAGG+TGG	0.538687	7.4:-26760198	MS.gene058677:CDS
CAACAATAACTTGCTTGTTG+GGG	0.541537	7.4:-26760346	MS.gene058677:CDS
AAGAAGGTGTAAAGAAGAAG+GGG	0.545916	7.4:+26760445	None:intergenic
CCTTTGGAATCTCAAGATCA+AGG	0.550549	7.4:-26760411	MS.gene058677:CDS
GGTATATTCATTAATGGTGG+TGG	0.558354	7.4:+26760501	None:intergenic
CTTGATCTTGAGATTCCAAA+GGG	0.558938	7.4:+26760412	None:intergenic
CTTGTCTGAAATGCAAACCT+TGG	0.566555	7.4:+26760150	None:intergenic
TCAAGAACAGCAAGTATCCC+AGG	0.569780	7.4:-26760068	MS.gene058677:CDS
AGGCCTTGGTGATATTGAGT+GGG	0.589628	7.4:-26760391	MS.gene058677:CDS
TAATTCAAAATAATGAAAGT+GGG	0.605922	7.4:-26760260	MS.gene058677:CDS
AAGGCCTTGGTGATATTGAG+TGG	0.607465	7.4:-26760392	MS.gene058677:CDS
CCTTGATCTTGAGATTCCAA+AGG	0.608143	7.4:+26760411	None:intergenic
GATGGTATATTCATTAATGG+TGG	0.631790	7.4:+26760498	None:intergenic
AAAAGACAACAAAAGTGCCA+AGG	0.641958	7.4:-26760167	MS.gene058677:CDS
CATACATGTAGTATATACCT+GGG	0.661629	7.4:+26760051	None:intergenic
GGCCTTGGTGATATTGAGTG+GGG	0.673375	7.4:-26760390	MS.gene058677:CDS
TTCCCCACTCAATATCACCA+AGG	0.720297	7.4:+26760388	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATTCAAAATAATGAAAGT+GGG	-	chr7.4:26760312-26760331	MS.gene058677:CDS	15.0%
!!	GTAATTCAAAATAATGAAAG+TGG	-	chr7.4:26760311-26760330	MS.gene058677:CDS	20.0%
!!!	AAGAAGTGTTGATGATATTT+TGG	-	chr7.4:26760442-26760461	MS.gene058677:CDS	25.0%
	AAACAAGTTAGAGGAGAAAA+GGG	-	chr7.4:26760364-26760383	MS.gene058677:CDS	30.0%
	ACATACATGTAGTATATACC+TGG	+	chr7.4:26760525-26760544	None:intergenic	30.0%
	CAACTACATGAAGAAGAAAA+GGG	-	chr7.4:26760341-26760360	MS.gene058677:CDS	30.0%
	CACTTCATCTTCTTCTAATT+TGG	-	chr7.4:26760289-26760308	MS.gene058677:CDS	30.0%
	CATACATGTAGTATATACCT+GGG	+	chr7.4:26760524-26760543	None:intergenic	30.0%
	GATGGTATATTCATTAATGG+TGG	+	chr7.4:26760077-26760096	None:intergenic	30.0%
	TCAACTACATGAAGAAGAAA+AGG	-	chr7.4:26760340-26760359	MS.gene058677:CDS	30.0%
	TTCTTTACAAAACTACCCTT+TGG	-	chr7.4:26760145-26760164	MS.gene058677:CDS	30.0%
!	AAAGAAGGTGTAAAGAAGAA+GGG	+	chr7.4:26760131-26760150	None:intergenic	30.0%
!	GGTGATGGTATATTCATTAA+TGG	+	chr7.4:26760080-26760099	None:intergenic	30.0%
!	TAAAGAAGGTGTAAAGAAGA+AGG	+	chr7.4:26760132-26760151	None:intergenic	30.0%
!!	AACAACAATAACTTGCTTGT+TGG	-	chr7.4:26760224-26760243	MS.gene058677:CDS	30.0%
!!	ACAACAATAACTTGCTTGTT+GGG	-	chr7.4:26760225-26760244	MS.gene058677:CDS	30.0%
!!!	AAAGGGTAGTTTTGTAAAGA+AGG	+	chr7.4:26760146-26760165	None:intergenic	30.0%
!!!	GTTGATGATATTTTGGATGA+TGG	-	chr7.4:26760449-26760468	MS.gene058677:CDS	30.0%
	AGAAAAGGGAAACAAGTTAG+AGG	-	chr7.4:26760355-26760374	MS.gene058677:CDS	35.0%
	CTTGATCTTGAGATTCCAAA+GGG	+	chr7.4:26760163-26760182	None:intergenic	35.0%
	GAAACAAGTTAGAGGAGAAA+AGG	-	chr7.4:26760363-26760382	MS.gene058677:CDS	35.0%
	GGTATATTCATTAATGGTGG+TGG	+	chr7.4:26760074-26760093	None:intergenic	35.0%
!	AAGAAGGTGTAAAGAAGAAG+GGG	+	chr7.4:26760130-26760149	None:intergenic	35.0%
!!	AAAAGACAACAAAAGTGCCA+AGG	-	chr7.4:26760405-26760424	MS.gene058677:CDS	35.0%
!!	ATATTCATTAATGGTGGTGG+TGG	+	chr7.4:26760071-26760090	None:intergenic	35.0%
!!	CAACAATAACTTGCTTGTTG+GGG	-	chr7.4:26760226-26760245	MS.gene058677:CDS	35.0%
!!	TATTCATTAATGGTGGTGGT+GGG	+	chr7.4:26760070-26760089	None:intergenic	35.0%
!!	TTTTGGATGATGGTTATCGT+TGG	-	chr7.4:26760459-26760478	MS.gene058677:CDS	35.0%
!!!	GTGTTGTTGTTTTGAGTTTG+TGG	+	chr7.4:26760101-26760120	None:intergenic	35.0%
!!!	TTGTTTTGAGTTTGTGGTGA+TGG	+	chr7.4:26760095-26760114	None:intergenic	35.0%
	CCTTGATCTTGAGATTCCAA+AGG	+	chr7.4:26760164-26760183	None:intergenic	40.0%
	GGTTATCGTTGGAGGAAATA+TGG	-	chr7.4:26760470-26760489	MS.gene058677:CDS	40.0%
	TTAGAGGAGAAAAGGGTTAG+AGG	-	chr7.4:26760371-26760390	MS.gene058677:CDS	40.0%
!	CTTGTCTGAAATGCAAACCT+TGG	+	chr7.4:26760425-26760444	None:intergenic	40.0%
!!	CCTTTGGAATCTCAAGATCA+AGG	-	chr7.4:26760161-26760180	MS.gene058677:CDS	40.0%
!!	GAGTGGGGAAACTTTTTCTT+AGG	-	chr7.4:26760197-26760216	MS.gene058677:CDS	40.0%
	AGGAGAAAAGGGTTAGAGGT+GGG	-	chr7.4:26760375-26760394	MS.gene058677:CDS	45.0%
	GAATCTCAAGATCAAGGCCT+TGG	-	chr7.4:26760167-26760186	MS.gene058677:CDS	45.0%
	TCAAGAACAGCAAGTATCCC+AGG	-	chr7.4:26760504-26760523	MS.gene058677:CDS	45.0%
	TTCCCCACTCAATATCACCA+AGG	+	chr7.4:26760187-26760206	None:intergenic	45.0%
!	AAGGCCTTGGTGATATTGAG+TGG	-	chr7.4:26760180-26760199	MS.gene058677:CDS	45.0%
!	AGGCCTTGGTGATATTGAGT+GGG	-	chr7.4:26760181-26760200	MS.gene058677:CDS	45.0%
!	TGGATGATGGTTATCGTTGG+AGG	-	chr7.4:26760462-26760481	MS.gene058677:CDS	45.0%
!!	ATTAATGGTGGTGGTGGGTT+TGG	+	chr7.4:26760065-26760084	None:intergenic	45.0%
	GAGGAGAAAAGGGTTAGAGG+TGG	-	chr7.4:26760374-26760393	MS.gene058677:CDS	50.0%
!	CTTGCTTGTTGGGGATGCTA+AGG	-	chr7.4:26760235-26760254	MS.gene058677:CDS	50.0%
!	GGCCTTGGTGATATTGAGTG+GGG	-	chr7.4:26760182-26760201	MS.gene058677:CDS	50.0%
!!	AATGGTGGTGGTGGGTTTGG+TGG	+	chr7.4:26760062-26760081	None:intergenic	55.0%
!!	ATGGTGGTGGTGGGTTTGGT+GGG	+	chr7.4:26760061-26760080	None:intergenic	55.0%
!!	GTGGTGGGTTTGGTGGGTCT+TGG	+	chr7.4:26760055-26760074	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	26760044	26760550	26760044	ID=MS.gene058677
chr7.4	mRNA	26760044	26760550	26760044	ID=MS.gene058677.t1;Parent=MS.gene058677
chr7.4	exon	26760044	26760550	26760044	ID=MS.gene058677.t1.exon1;Parent=MS.gene058677.t1
chr7.4	CDS	26760044	26760550	26760044	ID=cds.MS.gene058677.t1;Parent=MS.gene058677.t1

Gene Sequence

>MS.gene058677

ATGGATAGCCAAGACCCACCAAACCCACCACCACCATTAATGAATATACCATCACCACAAACTCAAAACAACAACACTTCTCTCCCCTTCTTCTTTACACCTTCTTTACAAAACTACCCTTTGGAATCTCAAGATCAAGGCCTTGGTGATATTGAGTGGGGAAACTTTTTCTTAGGTCAAAACAACAATAACTTGCTTGTTGGGGATGCTAAGGAGACATTAAAGATTGACAATATTCAACAATGCACTTCATCTTCTTCTAATTTGGTAATTCAAAATAATGAAAGTGGGAGTTATCAACTACATGAAGAAGAAAAGGGAAACAAGTTAGAGGAGAAAAGGGTTAGAGGTGGGAGAGTGAAAAAGACAACAAAAGTGCCAAGGTTTGCATTTCAGACAAGAAGTGTTGATGATATTTTGGATGATGGTTATCGTTGGAGGAAATATGGACAAAAAGCTGTCAAGAACAGCAAGTATCCCAGGTATATACTACATGTATGTTTGTGA

Protein sequence

>MS.gene058677.t1

MDSQDPPNPPPPLMNIPSPQTQNNNTSLPFFFTPSLQNYPLESQDQGLGDIEWGNFFLGQNNNNLLVGDAKETLKIDNIQQCTSSSSNLVIQNNESGSYQLHEEEKGNKLEEKRVRGGRVKKTTKVPRFAFQTRSVDDILDDGYRWRKYGQKAVKNSKYPRYILHVCL