Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058707.t1 | XP_024626251.1 | 97.7 | 87 | 2 | 0 | 1 | 87 | 1 | 87 | 9.50E-36 | 159.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058707.t1 | A2Q1P9 | 97.7 | 87 | 2 | 0 | 1 | 87 | 1 | 87 | 6.8e-36 | 159.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058706 | MS.gene058707 | 0.805083 | 1.58E-49 | -1.69E-46 |
| MS.gene058707 | MS.gene76208 | 0.806866 | 6.68E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058707.t1 | AT4G30390 | 65.476 | 84 | 29 | 0 | 4 | 87 | 5 | 88 | 3.22e-36 | 118 |
Find 17 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGAGATGTCGGAGAGATTA+AGG | 0.252011 | 7.4:-26413285 | None:intergenic |
| AACTCACTGGTAGGAAGAAC+AGG | 0.344126 | 7.4:-26414497 | None:intergenic |
| CTGAAACTGTTTATAGCAAC+TGG | 0.365834 | 7.4:+26414419 | MS.gene058707:CDS |
| CATTCTTCTAGATCTCTTCC+TGG | 0.422448 | 7.4:+26413330 | MS.gene058707:CDS |
| AGATCTAGAAGAATGTGATC+AGG | 0.429631 | 7.4:-26413322 | None:intergenic |
| ATTAAGGACGGCTGAGTCAA+TGG | 0.488201 | 7.4:-26413269 | None:intergenic |
| ATCAGGGATGAGAGAGAGAT+CGG | 0.511014 | 7.4:-26413305 | None:intergenic |
| GAGAGAGAGATCGGAGATGT+CGG | 0.529629 | 7.4:-26413296 | None:intergenic |
| GTGCAGAGAATATTGAAAGC+TGG | 0.536542 | 7.4:+26414377 | MS.gene058707:intron |
| GTTTATAGCAACTGGTAATG+AGG | 0.537844 | 7.4:+26414427 | MS.gene058707:CDS |
| GATCTAGAAGAATGTGATCA+GGG | 0.580760 | 7.4:-26413321 | None:intergenic |
| ATGGTGAGAGAAAGTAATGA+TGG | 0.588762 | 7.4:-26413250 | None:intergenic |
| GAACAGGGGTCACAATATGT+CGG | 0.636120 | 7.4:-26414481 | None:intergenic |
| GATGTCGGAGAGATTAAGGA+CGG | 0.674146 | 7.4:-26413281 | None:intergenic |
| ACTCACTGGTAGGAAGAACA+GGG | 0.694442 | 7.4:-26414496 | None:intergenic |
| GTGAGAGAAAGTAATGATGG+CGG | 0.736980 | 7.4:-26413247 | None:intergenic |
| CTCACTGGTAGGAAGAACAG+GGG | 0.761648 | 7.4:-26414495 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTTGTAAATTATTATAC+AGG | + | chr7.4:26414583-26414602 | MS.gene058707:intron | 10.0% |
| !!! | AAAATTTTATCATGTATTTA+TGG | + | chr7.4:26414525-26414544 | MS.gene058707:intron | 10.0% |
| !! | AAATAAAGAAAAATTGAGAA+AGG | - | chr7.4:26413420-26413439 | None:intergenic | 15.0% |
| !! | AATAAAGAAAAATTGAGAAA+GGG | - | chr7.4:26413419-26413438 | None:intergenic | 15.0% |
| !! | AATTAATCAAGAAAAACAAA+AGG | - | chr7.4:26413462-26413481 | None:intergenic | 15.0% |
| !! | ATTAATCAAGAAAAACAAAA+GGG | - | chr7.4:26413461-26413480 | None:intergenic | 15.0% |
| !! | TTACTTATGATTATAGAATA+TGG | - | chr7.4:26413539-26413558 | None:intergenic | 15.0% |
| !!! | AAAATTTTAAAATAACTCAC+TGG | - | chr7.4:26414513-26414532 | None:intergenic | 15.0% |
| !!! | ATACTCATTTATCTTTTTTA+TGG | + | chr7.4:26413985-26414004 | MS.gene058707:intron | 15.0% |
| !!! | TACTCATTTATCTTTTTTAT+GGG | + | chr7.4:26413986-26414005 | MS.gene058707:intron | 15.0% |
| !! | ACTTAATTATAGACAACAAA+AGG | - | chr7.4:26414275-26414294 | None:intergenic | 20.0% |
| !! | ATGATTATAGAATATGGATA+AGG | - | chr7.4:26413533-26413552 | None:intergenic | 20.0% |
| !! | CATACTTATTTAATCAGATA+AGG | - | chr7.4:26414158-26414177 | None:intergenic | 20.0% |
| !! | CTTATCTGATTAAATAAGTA+TGG | + | chr7.4:26414156-26414175 | MS.gene058707:intron | 20.0% |
| !!! | ACTCATTTATCTTTTTTATG+GGG | + | chr7.4:26413987-26414006 | MS.gene058707:intron | 20.0% |
| !!! | TCATTTTCTCAAATTATTAC+AGG | - | chr7.4:26413717-26413736 | None:intergenic | 20.0% |
| !!! | TGTTTAAATTGATTTGTCTT+TGG | + | chr7.4:26413636-26413655 | MS.gene058707:intron | 20.0% |
| ! | GATTTGATATATTGTAGATC+TGG | + | chr7.4:26414242-26414261 | MS.gene058707:intron | 25.0% |
| ! | TTACAATTACTCTTGTAGTA+TGG | + | chr7.4:26413595-26413614 | MS.gene058707:intron | 25.0% |
| !! | TAATGTTGTTTGCTTCTTTA+GGG | + | chr7.4:26413561-26413580 | MS.gene058707:intron | 25.0% |
| !! | TACCTTGTTCTTTTTCTATT+GGG | + | chr7.4:26414076-26414095 | MS.gene058707:intron | 25.0% |
| !! | TATGCTAAAGTTTCTTCTTT+TGG | + | chr7.4:26414311-26414330 | MS.gene058707:intron | 25.0% |
| !!! | CAGGATTTTGATTCATAATT+TGG | + | chr7.4:26414220-26414239 | MS.gene058707:intron | 25.0% |
| !!! | TTTTAAAATAACTCACTGGT+AGG | - | chr7.4:26414509-26414528 | None:intergenic | 25.0% |
| AGATTGTAGTTGGATCATTT+AGG | + | chr7.4:26413908-26413927 | MS.gene058707:intron | 30.0% | |
| GAAAATTAAGAAATGCGATG+AGG | - | chr7.4:26414354-26414373 | None:intergenic | 30.0% | |
| GAGAGAATAAAAAGAATACC+AGG | - | chr7.4:26413351-26413370 | None:intergenic | 30.0% | |
| GTAGTTACTACTAGCTAATA+AGG | + | chr7.4:26413390-26413409 | MS.gene058707:intron | 30.0% | |
| TATGAATCAAAATCCTGACA+AGG | - | chr7.4:26414217-26414236 | None:intergenic | 30.0% | |
| TTGTCTTTGGATGAATTTGT+TGG | + | chr7.4:26413649-26413668 | MS.gene058707:intron | 30.0% | |
| ! | GTAATGTTGTTTGCTTCTTT+AGG | + | chr7.4:26413560-26413579 | MS.gene058707:intron | 30.0% |
| ! | GTACCTTGTTCTTTTTCTAT+TGG | + | chr7.4:26414075-26414094 | MS.gene058707:intron | 30.0% |
| !! | CATCATTTTCTTATTTGGGA+GGG | + | chr7.4:26414033-26414052 | MS.gene058707:intron | 30.0% |
| !! | GTGCCATCATTTTCTTATTT+GGG | + | chr7.4:26414029-26414048 | MS.gene058707:intron | 30.0% |
| AATTAAGAAATGCGATGAGG+AGG | - | chr7.4:26414351-26414370 | None:intergenic | 35.0% | |
| AGATCTAGAAGAATGTGATC+AGG | - | chr7.4:26413325-26413344 | None:intergenic | 35.0% | |
| AGCCCAATAGAAAAAGAACA+AGG | - | chr7.4:26414081-26414100 | None:intergenic | 35.0% | |
| ATCTGAGAACAACTAAGACT+TGG | + | chr7.4:26413942-26413961 | MS.gene058707:intron | 35.0% | |
| ATGGTGAGAGAAAGTAATGA+TGG | - | chr7.4:26413253-26413272 | None:intergenic | 35.0% | |
| CCTCCCAAATAAGAAAATGA+TGG | - | chr7.4:26414035-26414054 | None:intergenic | 35.0% | |
| CTGAAACTGTTTATAGCAAC+TGG | + | chr7.4:26414419-26414438 | MS.gene058707:CDS | 35.0% | |
| CTTTCAATATTCTCTGCACA+TGG | - | chr7.4:26414376-26414395 | None:intergenic | 35.0% | |
| GATCTAGAAGAATGTGATCA+GGG | - | chr7.4:26413324-26413343 | None:intergenic | 35.0% | |
| GTCAAATCTTTACAGCATCT+GGG | - | chr7.4:26414136-26414155 | None:intergenic | 35.0% | |
| GTGAAAGTTGTTGTCAAATG+TGG | + | chr7.4:26413828-26413847 | MS.gene058707:intron | 35.0% | |
| TACGAATGAAGTTTGAGAGA+GGG | - | chr7.4:26413373-26413392 | None:intergenic | 35.0% | |
| TAGAATATGGATAAGGTCAC+AGG | - | chr7.4:26413526-26413545 | None:intergenic | 35.0% | |
| TCAAATCTTTACAGCATCTG+GGG | - | chr7.4:26414135-26414154 | None:intergenic | 35.0% | |
| TCTGAGAACAACTAAGACTT+GGG | + | chr7.4:26413943-26413962 | MS.gene058707:intron | 35.0% | |
| ! | GGGTTGAATTGATAGTTATG+TGG | + | chr7.4:26414007-26414026 | MS.gene058707:intron | 35.0% |
| ! | GTTTATAGCAACTGGTAATG+AGG | + | chr7.4:26414427-26414446 | MS.gene058707:CDS | 35.0% |
| ! | TTTTCAGCAGCCATAGAATT+TGG | + | chr7.4:26414191-26414210 | MS.gene058707:intron | 35.0% |
| !! | CCATCATTTTCTTATTTGGG+AGG | + | chr7.4:26414032-26414051 | MS.gene058707:intron | 35.0% |
| !! | GGTGCCATCATTTTCTTATT+TGG | + | chr7.4:26414028-26414047 | MS.gene058707:intron | 35.0% |
| CACAATGTGCACGCAATTAA+GGG | - | chr7.4:26413862-26413881 | None:intergenic | 40.0% | |
| CATTCTTCTAGATCTCTTCC+TGG | + | chr7.4:26413330-26413349 | MS.gene058707:CDS | 40.0% | |
| CTACGAATGAAGTTTGAGAG+AGG | - | chr7.4:26413374-26413393 | None:intergenic | 40.0% | |
| GCACTAACACCTCAAATCAA+AGG | - | chr7.4:26413772-26413791 | None:intergenic | 40.0% | |
| GCTGCTGAAAAATTGTCAAC+AGG | - | chr7.4:26414182-26414201 | None:intergenic | 40.0% | |
| GGTCAAATCTTTACAGCATC+TGG | - | chr7.4:26414137-26414156 | None:intergenic | 40.0% | |
| GTGAGAGAAAGTAATGATGG+CGG | - | chr7.4:26413250-26413269 | None:intergenic | 40.0% | |
| GTGCAGAGAATATTGAAAGC+TGG | + | chr7.4:26414377-26414396 | MS.gene058707:intron | 40.0% | |
| GTTGCAGTCTAGATTGTAGT+TGG | + | chr7.4:26413898-26413917 | MS.gene058707:intron | 40.0% | |
| ! | ATTTGAGGTGTTAGTGCTAG+AGG | + | chr7.4:26413775-26413794 | MS.gene058707:intron | 40.0% |
| !! | GTTGACACACCTTTGATTTG+AGG | + | chr7.4:26413760-26413779 | MS.gene058707:intron | 40.0% |
| AACTCACTGGTAGGAAGAAC+AGG | - | chr7.4:26414500-26414519 | None:intergenic | 45.0% | |
| ACTCACTGGTAGGAAGAACA+GGG | - | chr7.4:26414499-26414518 | None:intergenic | 45.0% | |
| AGTGCTAGAGGTTGTGTACA+AGG | + | chr7.4:26413787-26413806 | MS.gene058707:intron | 45.0% | |
| ATCAGGGATGAGAGAGAGAT+CGG | - | chr7.4:26413308-26413327 | None:intergenic | 45.0% | |
| ATTAAGGACGGCTGAGTCAA+TGG | - | chr7.4:26413272-26413291 | None:intergenic | 45.0% | |
| CCACAATGTGCACGCAATTA+AGG | - | chr7.4:26413863-26413882 | None:intergenic | 45.0% | |
| CCTGACAAGGCCAAATTCTA+TGG | - | chr7.4:26414204-26414223 | None:intergenic | 45.0% | |
| CCTTAATTGCGTGCACATTG+TGG | + | chr7.4:26413860-26413879 | MS.gene058707:intron | 45.0% | |
| GAACAGGGGTCACAATATGT+CGG | - | chr7.4:26414484-26414503 | None:intergenic | 45.0% | |
| GATGTCGGAGAGATTAAGGA+CGG | - | chr7.4:26413284-26413303 | None:intergenic | 45.0% | |
| !! | CCATAGAATTTGGCCTTGTC+AGG | + | chr7.4:26414201-26414220 | MS.gene058707:intron | 45.0% |
| CGGAGATGTCGGAGAGATTA+AGG | - | chr7.4:26413288-26413307 | None:intergenic | 50.0% | |
| CTCACTGGTAGGAAGAACAG+GGG | - | chr7.4:26414498-26414517 | None:intergenic | 50.0% | |
| GAGAGAGAGATCGGAGATGT+CGG | - | chr7.4:26413299-26413318 | None:intergenic | 50.0% | |
| TTGCGTGCACATTGTGGCTG+CGG | + | chr7.4:26413866-26413885 | MS.gene058707:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26413238 | 26414624 | 26413238 | ID=MS.gene058707 |
| chr7.4 | mRNA | 26413238 | 26414624 | 26413238 | ID=MS.gene058707.t1;Parent=MS.gene058707 |
| chr7.4 | exon | 26413238 | 26413351 | 26413238 | ID=MS.gene058707.t1.exon1;Parent=MS.gene058707.t1 |
| chr7.4 | CDS | 26413238 | 26413351 | 26413238 | ID=cds.MS.gene058707.t1;Parent=MS.gene058707.t1 |
| chr7.4 | exon | 26414383 | 26414512 | 26414383 | ID=MS.gene058707.t1.exon2;Parent=MS.gene058707.t1 |
| chr7.4 | CDS | 26414383 | 26414512 | 26414383 | ID=cds.MS.gene058707.t1;Parent=MS.gene058707.t1 |
| chr7.4 | exon | 26414605 | 26414624 | 26414605 | ID=MS.gene058707.t1.exon3;Parent=MS.gene058707.t1 |
| chr7.4 | CDS | 26414605 | 26414624 | 26414605 | ID=cds.MS.gene058707.t1;Parent=MS.gene058707.t1 |
| Gene Sequence |
| Protein sequence |
