AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058998

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058998.t1 MTR_1g069015 89.637 386 40 0 1 386 88 473 0.0 708
MS.gene058998.t1 MTR_1g069020 67.347 343 100 3 44 386 246 576 8.71e-144 421
MS.gene058998.t1 MTR_1g069020 54.962 262 104 3 1 259 92 342 7.19e-83 265
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 90 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCATCAGATGATATTTC+TGG 0.152483 1.2:+51728689 None:intergenic
TTCTACCTCGCTTAGAGTTT+TGG 0.216024 1.2:-51727774 MS.gene058998:CDS
CTCTGTTTCTCCAGAGTCAT+TGG 0.218790 1.2:-51728030 MS.gene058998:CDS
CGGTAACAGGCTGTTTCTTC+TGG 0.258466 1.2:+51728384 None:intergenic
GATTAACAGGGCAGAGATTT+AGG 0.261531 1.2:-51727192 MS.gene058998:intron
TCAAACACCTATTTATAATA+TGG 0.307116 1.2:-51727746 MS.gene058998:intron
GAGGTTGTTATAAGAGAAAA+AGG 0.309998 1.2:-51728175 MS.gene058998:CDS
CGGAAGCAGCAGCCTGCATC+TGG 0.314800 1.2:-51728815 MS.gene058998:CDS
GAGCACCGCACGCTGGAAAA+TGG 0.319034 1.2:-51729431 MS.gene058998:CDS
TCACATACCATATTATAAAT+AGG 0.323614 1.2:+51727739 None:intergenic
TCTTTATCTGGATGTGTTGC+CGG 0.325220 1.2:+51728356 None:intergenic
AGAGCATAGCTTTCCCAGTT+TGG 0.337878 1.2:+51729224 None:intergenic
TGATTCGGCCAATGCTGTTC+CGG 0.339045 1.2:-51729168 MS.gene058998:CDS
GGAATAGTAAACACATTGTC+TGG 0.341105 1.2:-51728254 MS.gene058998:CDS
GTGGAGATCTGGTCTTTATC+TGG 0.363337 1.2:+51728344 None:intergenic
AGTGGGGTTGATGTTACTTA+TGG 0.365193 1.2:-51728890 MS.gene058998:CDS
CAGAAGAAACAGCCTGTTAC+CGG 0.388877 1.2:-51728383 MS.gene058998:CDS
AGAGTTTAACATTATGCAAC+CGG 0.398439 1.2:+51728408 None:intergenic
AACCCCACTCATCTCCTTTG+AGG 0.416310 1.2:+51728904 None:intergenic
GGTGTTGCCTGACGAGGCCC+TGG 0.428575 1.2:-51728966 MS.gene058998:CDS
GGGCAGAGATTTAGGCATAA+TGG 0.434677 1.2:-51727184 MS.gene058998:CDS
TCTGTTTCTCCAGAGTCATT+GGG 0.438436 1.2:-51728029 MS.gene058998:CDS
CAATCTCCCGTGGTCTTATC+CGG 0.440181 1.2:+51728524 None:intergenic
TCCTACTTCTTTGGTATCAC+CGG 0.443039 1.2:-51728543 MS.gene058998:CDS
GGCATTCCTCGAAGATGAAC+GGG 0.446700 1.2:-51728495 MS.gene058998:CDS
GGTGAGGTCAATGTTGTTCA+TGG 0.450542 1.2:-51728455 MS.gene058998:CDS
GACACCCATTCAGTCACAAA+AGG 0.454994 1.2:-51728276 MS.gene058998:CDS
GTTGCCGGATCTCCGGTAAC+AGG 0.455494 1.2:+51728371 None:intergenic
ACATGTCCCGTTCATCTTCG+AGG 0.466823 1.2:+51728489 None:intergenic
AGGCTGGTAAGGTGGAGATC+TGG 0.472582 1.2:+51728333 None:intergenic
GATCTCCACCTTACCAGCCT+TGG 0.478185 1.2:-51728330 MS.gene058998:CDS
ACAGCCTGTTACCGGAGATC+CGG 0.478659 1.2:-51728375 MS.gene058998:CDS
GACCACGGGAGATTGCAATA+AGG 0.479142 1.2:-51728516 MS.gene058998:CDS
TGGATGTGTTGCCGGATCTC+CGG 0.481700 1.2:+51728364 None:intergenic
ATCTGAAGCTGATATTGATT+CGG 0.486466 1.2:-51729183 MS.gene058998:CDS
GCTCTAGAATGCACCGGAGA+AGG 0.500619 1.2:-51729206 MS.gene058998:CDS
AAATTTCATTCCTACTTCGT+TGG 0.500725 1.2:-51728987 MS.gene058998:CDS
GTCAATGTTGTTCATGGTAG+TGG 0.510692 1.2:-51728449 MS.gene058998:CDS
AGAAGTGGAAACTCGTGATT+CGG 0.511753 1.2:-51728747 MS.gene058998:CDS
CTGTAAAATCTCCACAAGGA+AGG 0.520970 1.2:-51728094 MS.gene058998:CDS
TACACTATCCGGAACAGCAT+TGG 0.528438 1.2:+51729160 None:intergenic
CTACGATGACCTCCAGATGC+AGG 0.528905 1.2:+51728803 None:intergenic
ACACCCATTCAGTCACAAAA+GGG 0.532185 1.2:-51728275 MS.gene058998:CDS
GTTGATGTTACTTATGGTAG+TGG 0.532246 1.2:-51728884 MS.gene058998:CDS
GGAAAATGCAAGAAACCATT+CGG 0.536222 1.2:-51728944 MS.gene058998:CDS
AAAAGAATGCAAAGCCTCAA+AGG 0.537351 1.2:-51728918 MS.gene058998:CDS
TGTAAAATCTCCACAAGGAA+GGG 0.537405 1.2:-51728093 MS.gene058998:CDS
CAAATTGCAGATACACTATC+CGG 0.539970 1.2:+51729149 None:intergenic
AACATTAATAGCACGTAACC+TGG 0.544388 1.2:+51728844 None:intergenic
GAGCTCTACCTCAACAGAAG+TGG 0.545818 1.2:-51728762 MS.gene058998:CDS
AGTAAACACATTGTCTGGGC+AGG 0.549663 1.2:-51728249 MS.gene058998:CDS
TTCGTTGGTGTTGCCTGACG+AGG 0.551013 1.2:-51728972 MS.gene058998:CDS
ATGTTGTTCATGGTAGTGGC+AGG 0.553357 1.2:-51728445 MS.gene058998:CDS
ATCAGCTTCAGATCCTTCTC+CGG 0.554639 1.2:+51729193 None:intergenic
GCGAAAATAGACATGGTACC+AGG 0.560344 1.2:-51728862 MS.gene058998:CDS
AAGCCTCAAAGGAGATGAGT+GGG 0.564158 1.2:-51728907 MS.gene058998:CDS
GCATAGCTTTCCCAGTTTGG+AGG 0.565501 1.2:+51729227 None:intergenic
AAGCAGCAGCCTGCATCTGG+AGG 0.566088 1.2:-51728812 MS.gene058998:CDS
GCAGGAATAGACGTAGTGCC+CGG 0.566956 1.2:-51728427 MS.gene058998:CDS
TCAGGCAACACCAACGAAGT+AGG 0.568738 1.2:+51728977 None:intergenic
GGAAAAGAGTGATGGAGGAC+TGG 0.572017 1.2:-51728309 MS.gene058998:CDS
AGGCATTCCTCGAAGATGAA+CGG 0.572872 1.2:-51728496 MS.gene058998:CDS
TCCGGTGATACCAAAGAAGT+AGG 0.573504 1.2:+51728542 None:intergenic
GGATACAAGTCACTCGAACA+AGG 0.576987 1.2:-51728072 MS.gene058998:CDS
ACGAGTTTCCACTTCTGTTG+AGG 0.577453 1.2:+51728754 None:intergenic
CATCCAGAAATATCATCTGA+TGG 0.590199 1.2:-51728692 MS.gene058998:intron
AGCTATGCTCTAGAATGCAC+CGG 0.591217 1.2:-51729212 MS.gene058998:CDS
ACGGGACATGTCAATTAAGA+CGG 0.591957 1.2:-51728477 MS.gene058998:CDS
CGGGACATGTCAATTAAGAC+GGG 0.595490 1.2:-51728476 MS.gene058998:CDS
TGACTTGTATCCCTTCCTTG+TGG 0.599408 1.2:+51728083 None:intergenic
TGCCTTATTGCAATCTCCCG+TGG 0.601695 1.2:+51728514 None:intergenic
CATGAACGAGCACCGCACGC+TGG 0.604496 1.2:-51729438 MS.gene058998:CDS
GAATAGTAAACACATTGTCT+GGG 0.607705 1.2:-51728253 MS.gene058998:CDS
CCAGCCTTGGAAAAGAGTGA+TGG 0.608271 1.2:-51728317 MS.gene058998:CDS
GATACAAGTCACTCGAACAA+GGG 0.609110 1.2:-51728071 MS.gene058998:CDS
ACAGTTGAAACAGAAGCAGT+TGG 0.619629 1.2:-51728149 MS.gene058998:CDS
CTGCATCTGGAGGTCATCGT+AGG 0.620919 1.2:-51728802 MS.gene058998:CDS
GCCTTGGAAAAGAGTGATGG+AGG 0.621071 1.2:-51728314 MS.gene058998:CDS
GTATCACCGGATAAGACCAC+GGG 0.626364 1.2:-51728530 MS.gene058998:CDS
GTGTTGCCTGACGAGGCCCT+GGG 0.635492 1.2:-51728965 MS.gene058998:CDS
CATGTCAATTAAGACGGGTG+AGG 0.637260 1.2:-51728471 MS.gene058998:CDS
GAGTTTAACATTATGCAACC+GGG 0.641017 1.2:+51728409 None:intergenic
AAAGCCTCAAAGGAGATGAG+TGG 0.652543 1.2:-51728908 MS.gene058998:CDS
CAATGACTCTGGAGAAACAG+AGG 0.661940 1.2:+51728031 None:intergenic
GTGCTATTAATGTTTGTCAG+CGG 0.670687 1.2:-51728835 MS.gene058998:CDS
GGTATCACCGGATAAGACCA+CGG 0.680385 1.2:-51728531 MS.gene058998:CDS
TCTTCTGTAAAATCTCCACA+AGG 0.702540 1.2:-51728098 MS.gene058998:CDS
GGTAGTGGCGAAAATAGACA+TGG 0.702887 1.2:-51728869 MS.gene058998:CDS
TTGCGCCAAAACTCTAAGCG+AGG 0.703796 1.2:+51727769 None:intergenic
AGCCTCAAAGGAGATGAGTG+GGG 0.721063 1.2:-51728906 MS.gene058998:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCAAAGAATTTTTTATA+TGG - chr1.2:51727359-51727378 MS.gene058998:intron 10.0%
!! AATAAATCCTAGATATAAAA+TGG + chr1.2:51728959-51728978 None:intergenic 15.0%
!! ATATACAAAAATGCTAACAA+TGG + chr1.2:51728753-51728772 None:intergenic 20.0%
!! ATGCATATGATATCTAATAA+TGG + chr1.2:51728935-51728954 None:intergenic 20.0%
!! TCAAACACCTATTTATAATA+TGG - chr1.2:51728867-51728886 MS.gene058998:CDS 20.0%
!! TCACATACCATATTATAAAT+AGG + chr1.2:51728877-51728896 None:intergenic 20.0%
!!! ACAATCTATAATCTTTTAAC+TGG - chr1.2:51729020-51729039 MS.gene058998:intron 20.0%
!!! ATTGTTAGCATTTTTGTATA+TGG - chr1.2:51728752-51728771 MS.gene058998:CDS 20.0%
!!! ATTTATTTTCTCTTTAAGGT+GGG - chr1.2:51728972-51728991 MS.gene058998:CDS 20.0%
!!! GGTTTAATATTTTTTCTCTT+AGG + chr1.2:51728491-51728510 None:intergenic 20.0%
!!! TAGGATTTATTTTCTCTTTA+AGG - chr1.2:51728968-51728987 MS.gene058998:CDS 20.0%
!!! TGTCAAGAATTTTTTTTTGA+TGG - chr1.2:51727485-51727504 MS.gene058998:intron 20.0%
!!! TTTTTTGATGGTAAGTATTT+TGG - chr1.2:51727497-51727516 MS.gene058998:intron 20.0%
! AAAGAATAGAGTGAAGTAAA+TGG + chr1.2:51728790-51728809 None:intergenic 25.0%
! ACAAATGGTATATGATTAAC+AGG - chr1.2:51729408-51729427 MS.gene058998:intron 25.0%
! AGGTTGTTATAAGAGAAAAA+GGG - chr1.2:51728439-51728458 MS.gene058998:CDS 25.0%
! CAAAGGGAGATAATTAATTA+GGG - chr1.2:51729367-51729386 MS.gene058998:intron 25.0%
! CAAATGGTATATGATTAACA+GGG - chr1.2:51729409-51729428 MS.gene058998:intron 25.0%
! TCAAACAACGTCATAAAATA+AGG + chr1.2:51727291-51727310 None:intergenic 25.0%
! TCACTCTATTCTTTATGTTT+GGG - chr1.2:51728796-51728815 MS.gene058998:CDS 25.0%
! TCATATACCATTTGTATGTT+CGG + chr1.2:51729403-51729422 None:intergenic 25.0%
! TTCACTCTATTCTTTATGTT+TGG - chr1.2:51728795-51728814 MS.gene058998:CDS 25.0%
! TTGAAATCTAACCATAAAGT+AGG + chr1.2:51727346-51727365 None:intergenic 25.0%
!! ATGCATACCATTTTATATCT+AGG - chr1.2:51728949-51728968 MS.gene058998:CDS 25.0%
!! TCTTTTATATCACCTTTTAG+AGG - chr1.2:51729100-51729119 MS.gene058998:intron 25.0%
!! TTTTTATCAGAACTATCATG+TGG - chr1.2:51729184-51729203 MS.gene058998:CDS 25.0%
!!! GATTTATTTTCTCTTTAAGG+TGG - chr1.2:51728971-51728990 MS.gene058998:CDS 25.0%
!!! TGTTTTTTTTGCCTACTTTA+TGG - chr1.2:51727332-51727351 MS.gene058998:intron 25.0%
!!! TTATGTTTGGGTTATTTTGT+AGG - chr1.2:51728808-51728827 MS.gene058998:CDS 25.0%
!!! TTTTTTAAAGACCTGTAACA+AGG + chr1.2:51729272-51729291 None:intergenic 25.0%
AAAAATGCTAACAATGGCAT+TGG + chr1.2:51728747-51728766 None:intergenic 30.0%
AAAATGCTAACAATGGCATT+GGG + chr1.2:51728746-51728765 None:intergenic 30.0%
AAATTTCATTCCTACTTCGT+TGG - chr1.2:51727626-51727645 MS.gene058998:intron 30.0%
AAGGTTAGAGAGAATTAGTT+AGG + chr1.2:51729253-51729272 None:intergenic 30.0%
AGAGTTTAACATTATGCAAC+CGG + chr1.2:51728208-51728227 None:intergenic 30.0%
ATTACAACCGAACATACAAA+TGG - chr1.2:51729393-51729412 MS.gene058998:intron 30.0%
CAATCATTGTCAATTCATGA+AGG - chr1.2:51728637-51728656 MS.gene058998:intron 30.0%
CTTCATGAATTGACAATGAT+TGG + chr1.2:51728639-51728658 None:intergenic 30.0%
GAATAGTAAACACATTGTCT+GGG - chr1.2:51728360-51728379 MS.gene058998:CDS 30.0%
GAGATTATAGAAATTCAGGA+AGG - chr1.2:51729073-51729092 MS.gene058998:intron 30.0%
GAGGTTGTTATAAGAGAAAA+AGG - chr1.2:51728438-51728457 MS.gene058998:CDS 30.0%
GCAAAGGGAGATAATTAATT+AGG - chr1.2:51729366-51729385 MS.gene058998:intron 30.0%
TTACCATCAGATGATATTTC+TGG + chr1.2:51727927-51727946 None:intergenic 30.0%
! ACAGGTCTTTAAAAAATGAG+CGG - chr1.2:51729276-51729295 MS.gene058998:intron 30.0%
! ATCTGAAGCTGATATTGATT+CGG - chr1.2:51727430-51727449 MS.gene058998:intron 30.0%
!! GTCAAAACATTTCTAAGACA+TGG + chr1.2:51727546-51727565 None:intergenic 30.0%
!!! GTAGAGCTCTGTTTTTTATT+TGG + chr1.2:51727840-51727859 None:intergenic 30.0%
!!! TGAAAAAGTTTTGGGAATTC+TGG + chr1.2:51728408-51728427 None:intergenic 30.0%
!!! TTGGTTTTAATTGTAGTTGC+AGG - chr1.2:51727597-51727616 MS.gene058998:intron 30.0%
AAAAGAATGCAAAGCCTCAA+AGG - chr1.2:51727695-51727714 MS.gene058998:intron 35.0%
AACATTAATAGCACGTAACC+TGG + chr1.2:51727772-51727791 None:intergenic 35.0%
AAGGTGGGTAAGATAAACAA+AGG - chr1.2:51728987-51729006 MS.gene058998:CDS 35.0%
AATATATACCAGCCCAATGA+TGG + chr1.2:51729133-51729152 None:intergenic 35.0%
AGGTGGGTAAGATAAACAAA+GGG - chr1.2:51728988-51729007 MS.gene058998:CDS 35.0%
AGTCTCGATATATAAGTCGT+TGG - chr1.2:51727578-51727597 MS.gene058998:intron 35.0%
ATAAGTGTCAATGTCTTTCG+TGG - chr1.2:51727228-51727247 MS.gene058998:intron 35.0%
ATTCTCTCTAACCTTGTTAC+AGG - chr1.2:51729258-51729277 MS.gene058998:intron 35.0%
CAAATTGCAGATACACTATC+CGG + chr1.2:51727467-51727486 None:intergenic 35.0%
CACTTATTACAACAAACCTG+AGG + chr1.2:51727215-51727234 None:intergenic 35.0%
CATCCAGAAATATCATCTGA+TGG - chr1.2:51727921-51727940 MS.gene058998:intron 35.0%
CATGAATGACAATTCATGAG+AGG + chr1.2:51728706-51728725 None:intergenic 35.0%
CCTGAATTTCTATAATCTCG+CGG + chr1.2:51729072-51729091 None:intergenic 35.0%
GAGTTTAACATTATGCAACC+GGG + chr1.2:51728207-51728226 None:intergenic 35.0%
GATATCTAATAATGGCAGCT+GGG + chr1.2:51728927-51728946 None:intergenic 35.0%
GGAAAATGCAAGAAACCATT+CGG - chr1.2:51727669-51727688 MS.gene058998:intron 35.0%
GGAATAGTAAACACATTGTC+TGG - chr1.2:51728359-51728378 MS.gene058998:CDS 35.0%
GGTCTCAAAAAATCAAGATC+TGG - chr1.2:51728605-51728624 MS.gene058998:intron 35.0%
TCTTCTGTAAAATCTCCACA+AGG - chr1.2:51728515-51728534 MS.gene058998:CDS 35.0%
TGATATCTAATAATGGCAGC+TGG + chr1.2:51728928-51728947 None:intergenic 35.0%
TGTAAAATCTCCACAAGGAA+GGG - chr1.2:51728520-51728539 MS.gene058998:CDS 35.0%
TTTAATTGTAGTTGCAGGTG+AGG - chr1.2:51727602-51727621 MS.gene058998:intron 35.0%
! AGTCTTCGCTGAAAAAGTTT+TGG + chr1.2:51728417-51728436 None:intergenic 35.0%
! ATATGGTTTTCCTCCAAACT+GGG - chr1.2:51727376-51727395 MS.gene058998:intron 35.0%
! ATTCCCTTTTGTGACTGAAT+GGG + chr1.2:51728344-51728363 None:intergenic 35.0%
! GAAGTCTTTTCCTACTTCTT+TGG - chr1.2:51728061-51728080 MS.gene058998:CDS 35.0%
! GTCTTCGCTGAAAAAGTTTT+GGG + chr1.2:51728416-51728435 None:intergenic 35.0%
! GTGCTATTAATGTTTGTCAG+CGG - chr1.2:51727778-51727797 MS.gene058998:CDS 35.0%
! GTTGATGTTACTTATGGTAG+TGG - chr1.2:51727729-51727748 MS.gene058998:intron 35.0%
! TATATGGTTTTCCTCCAAAC+TGG - chr1.2:51727375-51727394 MS.gene058998:intron 35.0%
! TATTCCCTTTTGTGACTGAA+TGG + chr1.2:51728345-51728364 None:intergenic 35.0%
! TGGAGATTTTACAGAAGATG+CGG + chr1.2:51728513-51728532 None:intergenic 35.0%
! TTGCATTCTTTTGTACCGAA+TGG + chr1.2:51727687-51727706 None:intergenic 35.0%
!! GTTTGGGTTATTTTGTAGGA+AGG - chr1.2:51728812-51728831 MS.gene058998:CDS 35.0%
!!! AAAACTTTTTCAGCGAAGAC+TGG - chr1.2:51728416-51728435 MS.gene058998:CDS 35.0%
ACACCCATTCAGTCACAAAA+GGG - chr1.2:51728338-51728357 MS.gene058998:CDS 40.0%
ACAGTTGAAACAGAAGCAGT+TGG - chr1.2:51728464-51728483 MS.gene058998:CDS 40.0%
ACGGGACATGTCAATTAAGA+CGG - chr1.2:51728136-51728155 MS.gene058998:CDS 40.0%
AGAAGTGGAAACTCGTGATT+CGG - chr1.2:51727866-51727885 MS.gene058998:intron 40.0%
CCGCGAGATTATAGAAATTC+AGG - chr1.2:51729069-51729088 MS.gene058998:intron 40.0%
CTGTAAAATCTCCACAAGGA+AGG - chr1.2:51728519-51728538 MS.gene058998:CDS 40.0%
GATACAAGTCACTCGAACAA+GGG - chr1.2:51728542-51728561 MS.gene058998:CDS 40.0%
GATTAACAGGGCAGAGATTT+AGG - chr1.2:51729421-51729440 MS.gene058998:CDS 40.0%
GTCAATGTTGTTCATGGTAG+TGG - chr1.2:51728164-51728183 MS.gene058998:CDS 40.0%
TCCTACTTCTTTGGTATCAC+CGG - chr1.2:51728070-51728089 MS.gene058998:CDS 40.0%
TCTTTATCTGGATGTGTTGC+CGG + chr1.2:51728260-51728279 None:intergenic 40.0%
TGTTTGAGTGCTAACTGTGA+TGG - chr1.2:51727304-51727323 MS.gene058998:intron 40.0%
! ATGGTTTCTTGCATTTTCCC+AGG + chr1.2:51727668-51727687 None:intergenic 40.0%
! CTTTTTACATTTGACCACCC+AGG - chr1.2:51729045-51729064 MS.gene058998:intron 40.0%
! GGAGATTTTACAGAAGATGC+GGG + chr1.2:51728512-51728531 None:intergenic 40.0%
! TAAAACCATTTTCCAGCGTG+CGG + chr1.2:51727190-51727209 None:intergenic 40.0%
! TCTGTTTCTCCAGAGTCATT+GGG - chr1.2:51728584-51728603 MS.gene058998:intron 40.0%
! TGAAGTCTGAAGTGTGCAAA+GGG - chr1.2:51729351-51729370 MS.gene058998:intron 40.0%
! TGGTTTCTTGCATTTTCCCA+GGG + chr1.2:51727667-51727686 None:intergenic 40.0%
! TTCTACCTCGCTTAGAGTTT+TGG - chr1.2:51728839-51728858 MS.gene058998:CDS 40.0%
!! AGTGGGGTTGATGTTACTTA+TGG - chr1.2:51727723-51727742 MS.gene058998:intron 40.0%
!! GGAAAATGGTTTTACTCCTC+AGG - chr1.2:51727196-51727215 MS.gene058998:intron 40.0%
!! TGGGTTATTTTGTAGGAAGG+TGG - chr1.2:51728815-51728834 MS.gene058998:CDS 40.0%
!!! TTTTTGAGACCCAATGACTC+TGG + chr1.2:51728596-51728615 None:intergenic 40.0%
AAAGCCTCAAAGGAGATGAG+TGG - chr1.2:51727705-51727724 MS.gene058998:intron 45.0%
AAGCCTCAAAGGAGATGAGT+GGG - chr1.2:51727706-51727725 MS.gene058998:intron 45.0%
ACGAGTTTCCACTTCTGTTG+AGG + chr1.2:51727862-51727881 None:intergenic 45.0%
AGAGCATAGCTTTCCCAGTT+TGG + chr1.2:51727392-51727411 None:intergenic 45.0%
AGAGGCTCTTCACCATCATT+GGG - chr1.2:51729118-51729137 MS.gene058998:intron 45.0%
AGGCATTCCTCGAAGATGAA+CGG - chr1.2:51728117-51728136 MS.gene058998:CDS 45.0%
AGTAAACACATTGTCTGGGC+AGG - chr1.2:51728364-51728383 MS.gene058998:CDS 45.0%
ATCAGCTTCAGATCCTTCTC+CGG + chr1.2:51727423-51727442 None:intergenic 45.0%
CAGAAGAAACAGCCTGTTAC+CGG - chr1.2:51728230-51728249 MS.gene058998:CDS 45.0%
CATGTCAATTAAGACGGGTG+AGG - chr1.2:51728142-51728161 MS.gene058998:CDS 45.0%
CGGGACATGTCAATTAAGAC+GGG - chr1.2:51728137-51728156 MS.gene058998:CDS 45.0%
GACACCCATTCAGTCACAAA+AGG - chr1.2:51728337-51728356 MS.gene058998:CDS 45.0%
GATGGTGAAGAGCCTCTAAA+AGG + chr1.2:51729115-51729134 None:intergenic 45.0%
GCGAAAATAGACATGGTACC+AGG - chr1.2:51727751-51727770 MS.gene058998:CDS 45.0%
GGATACAAGTCACTCGAACA+AGG - chr1.2:51728541-51728560 MS.gene058998:CDS 45.0%
GGGCAGAGATTTAGGCATAA+TGG - chr1.2:51729429-51729448 MS.gene058998:CDS 45.0%
GGTGAGGTCAATGTTGTTCA+TGG - chr1.2:51728158-51728177 MS.gene058998:CDS 45.0%
GTGTCAATGTCTTTCGTGGT+TGG - chr1.2:51727232-51727251 MS.gene058998:intron 45.0%
TACACTATCCGGAACAGCAT+TGG + chr1.2:51727456-51727475 None:intergenic 45.0%
TAGAGGCTCTTCACCATCAT+TGG - chr1.2:51729117-51729136 MS.gene058998:intron 45.0%
TCCGGTGATACCAAAGAAGT+AGG + chr1.2:51728074-51728093 None:intergenic 45.0%
TTCTATAATCTCGCGGTCCT+GGG + chr1.2:51729065-51729084 None:intergenic 45.0%
TTTCTATAATCTCGCGGTCC+TGG + chr1.2:51729066-51729085 None:intergenic 45.0%
! ACTCTTTTCCAAGGCTGGTA+AGG + chr1.2:51728294-51728313 None:intergenic 45.0%
! CAATGACTCTGGAGAAACAG+AGG + chr1.2:51728585-51728604 None:intergenic 45.0%
! CTCTGTTTCTCCAGAGTCAT+TGG - chr1.2:51728583-51728602 MS.gene058998:intron 45.0%
! CTGAAGTCTGAAGTGTGCAA+AGG - chr1.2:51729350-51729369 MS.gene058998:intron 45.0%
! GGTAGTGGCGAAAATAGACA+TGG - chr1.2:51727744-51727763 MS.gene058998:intron 45.0%
! GTGGAGATCTGGTCTTTATC+TGG + chr1.2:51728272-51728291 None:intergenic 45.0%
! TCCTCCATCACTCTTTTCCA+AGG + chr1.2:51728303-51728322 None:intergenic 45.0%
! TGACTTGTATCCCTTCCTTG+TGG + chr1.2:51728533-51728552 None:intergenic 45.0%
!! AGCTATGCTCTAGAATGCAC+CGG - chr1.2:51727401-51727420 MS.gene058998:intron 45.0%
!! ATGTTGTTCATGGTAGTGGC+AGG - chr1.2:51728168-51728187 MS.gene058998:CDS 45.0%
!!! ACTTTTTCAGCGAAGACTGG+AGG - chr1.2:51728419-51728438 MS.gene058998:CDS 45.0%
AACCCCACTCATCTCCTTTG+AGG + chr1.2:51727712-51727731 None:intergenic 50.0%
ACATGTCCCGTTCATCTTCG+AGG + chr1.2:51728127-51728146 None:intergenic 50.0%
AGCCTCAAAGGAGATGAGTG+GGG - chr1.2:51727707-51727726 MS.gene058998:intron 50.0%
CCAGCCTTGGAAAAGAGTGA+TGG - chr1.2:51728296-51728315 MS.gene058998:CDS 50.0%
GACCACGGGAGATTGCAATA+AGG - chr1.2:51728097-51728116 MS.gene058998:CDS 50.0%
GAGCTCTACCTCAACAGAAG+TGG - chr1.2:51727851-51727870 MS.gene058998:intron 50.0%
GCATAGCTTTCCCAGTTTGG+AGG + chr1.2:51727389-51727408 None:intergenic 50.0%
GCCTTGGAAAAGAGTGATGG+AGG - chr1.2:51728299-51728318 MS.gene058998:CDS 50.0%
GGCATTCCTCGAAGATGAAC+GGG - chr1.2:51728118-51728137 MS.gene058998:CDS 50.0%
GGTATCACCGGATAAGACCA+CGG - chr1.2:51728082-51728101 MS.gene058998:CDS 50.0%
GTATCACCGGATAAGACCAC+GGG - chr1.2:51728083-51728102 MS.gene058998:CDS 50.0%
TCAGGCAACACCAACGAAGT+AGG + chr1.2:51727639-51727658 None:intergenic 50.0%
TTGCGCCAAAACTCTAAGCG+AGG + chr1.2:51728847-51728866 None:intergenic 50.0%
! CCATCACTCTTTTCCAAGGC+TGG + chr1.2:51728299-51728318 None:intergenic 50.0%
! CTTTTCCAAGGCTGGTAAGG+TGG + chr1.2:51728291-51728310 None:intergenic 50.0%
! GGAAAAGAGTGATGGAGGAC+TGG - chr1.2:51728304-51728323 MS.gene058998:CDS 50.0%
! TGATTCGGCCAATGCTGTTC+CGG - chr1.2:51727445-51727464 MS.gene058998:intron 50.0%
! TGCCTTATTGCAATCTCCCG+TGG + chr1.2:51728102-51728121 None:intergenic 50.0%
! TGGCATTGGGTGAGAAGTGT+TGG + chr1.2:51728733-51728752 None:intergenic 50.0%
!! CAATCTCCCGTGGTCTTATC+CGG + chr1.2:51728092-51728111 None:intergenic 50.0%
!! CGGTAACAGGCTGTTTCTTC+TGG + chr1.2:51728232-51728251 None:intergenic 50.0%
ACAGCCTGTTACCGGAGATC+CGG - chr1.2:51728238-51728257 MS.gene058998:CDS 55.0%
AGGCTGGTAAGGTGGAGATC+TGG + chr1.2:51728283-51728302 None:intergenic 55.0%
CTACGATGACCTCCAGATGC+AGG + chr1.2:51727813-51727832 None:intergenic 55.0%
CTGCATCTGGAGGTCATCGT+AGG - chr1.2:51727811-51727830 MS.gene058998:intron 55.0%
GATCTCCACCTTACCAGCCT+TGG - chr1.2:51728283-51728302 MS.gene058998:CDS 55.0%
GCAGGAATAGACGTAGTGCC+CGG - chr1.2:51728186-51728205 MS.gene058998:CDS 55.0%
GCTCTTCACCATCATTGGGC+TGG - chr1.2:51729122-51729141 MS.gene058998:intron 55.0%
TATAATCTCGCGGTCCTGGG+TGG + chr1.2:51729062-51729081 None:intergenic 55.0%
TGGATGTGTTGCCGGATCTC+CGG + chr1.2:51728252-51728271 None:intergenic 55.0%
!! GCTCTAGAATGCACCGGAGA+AGG - chr1.2:51727407-51727426 MS.gene058998:intron 55.0%
!! TTCGTTGGTGTTGCCTGACG+AGG - chr1.2:51727641-51727660 MS.gene058998:intron 55.0%
AAGCAGCAGCCTGCATCTGG+AGG - chr1.2:51727801-51727820 MS.gene058998:intron 60.0%
GTTGCCGGATCTCCGGTAAC+AGG + chr1.2:51728245-51728264 None:intergenic 60.0%
! CATTTTCCCAGGGCCTCGTC+AGG + chr1.2:51727657-51727676 None:intergenic 60.0%
! GAGCACCGCACGCTGGAAAA+TGG - chr1.2:51727182-51727201 MS.gene058998:CDS 60.0%
CGGAAGCAGCAGCCTGCATC+TGG - chr1.2:51727798-51727817 MS.gene058998:intron 65.0%
GTGTTGCCTGACGAGGCCCT+GGG - chr1.2:51727648-51727667 MS.gene058998:intron 65.0%
! GGTGTTGCCTGACGAGGCCC+TGG - chr1.2:51727647-51727666 MS.gene058998:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 51727176 51729459 51727176 ID=MS.gene058998
chr1.2 mRNA 51727176 51729459 51727176 ID=MS.gene058998.t1;Parent=MS.gene058998
chr1.2 exon 51729418 51729459 51729418 ID=MS.gene058998.t1.exon1;Parent=MS.gene058998.t1
chr1.2 CDS 51729418 51729459 51729418 ID=cds.MS.gene058998.t1;Parent=MS.gene058998.t1
chr1.2 exon 51729129 51729276 51729129 ID=MS.gene058998.t1.exon2;Parent=MS.gene058998.t1
chr1.2 CDS 51729129 51729276 51729129 ID=cds.MS.gene058998.t1;Parent=MS.gene058998.t1
chr1.2 exon 51728693 51729016 51728693 ID=MS.gene058998.t1.exon3;Parent=MS.gene058998.t1
chr1.2 CDS 51728693 51729016 51728693 ID=cds.MS.gene058998.t1;Parent=MS.gene058998.t1
chr1.2 exon 51728009 51728581 51728009 ID=MS.gene058998.t1.exon4;Parent=MS.gene058998.t1
chr1.2 CDS 51728009 51728581 51728009 ID=cds.MS.gene058998.t1;Parent=MS.gene058998.t1
chr1.2 exon 51727747 51727805 51727747 ID=MS.gene058998.t1.exon5;Parent=MS.gene058998.t1
chr1.2 CDS 51727747 51727805 51727747 ID=cds.MS.gene058998.t1;Parent=MS.gene058998.t1
chr1.2 exon 51727176 51727205 51727176 ID=MS.gene058998.t1.exon6;Parent=MS.gene058998.t1
chr1.2 CDS 51727176 51727205 51727176 ID=cds.MS.gene058998.t1;Parent=MS.gene058998.t1
Gene Sequence

>MS.gene058998

ATGAACGAGCACCGCACGCTGGAAAATGGTTTTACTCCTCAGGTTTGTTGTAATAAGTGTCAATGTCTTTCGTGGTTGGTTTTGCAAGACGTTATTTATAATGATATTTGTTCCTTATTTTATGACGTTGTTTGAGTGCTAACTGTGATGGATTTTTGTTTTTTTTGCCTACTTTATGGTTAGATTTCAAAGAATTTTTTATATGGTTTTCCTCCAAACTGGGAAAGCTATGCTCTAGAATGCACCGGAGAAGGATCTGAAGCTGATATTGATTCGGCCAATGCTGTTCCGGATAGTGTATCTGCAATTTGTCAAGAATTTTTTTTTGATGGTAAGTATTTTGGTGATGTTTGTTAATTATTCTTGTCCATGTCTTAGAAATGTTTTGACGATTATCTCTTTAGTCTCGATATATAAGTCGTTGGTTTTAATTGTAGTTGCAGGTGAGGAAAATTTCATTCCTACTTCGTTGGTGTTGCCTGACGAGGCCCTGGGAAAATGCAAGAAACCATTCGGTACAAAAGAATGCAAAGCCTCAAAGGAGATGAGTGGGGTTGATGTTACTTATGGTAGTGGCGAAAATAGACATGGTACCAGGTTACGTGCTATTAATGTTTGTCAGCGGAAGCAGCAGCCTGCATCTGGAGGTCATCGTAGGCATCCAAATAAAAAACAGAGCTCTACCTCAACAGAAGTGGAAACTCGTGATTCGGATACTGCTATTTCTGAAAATGTATCAGTAAATCATCCAGAAATATCATCTGATGGTAAATATTTAGACATGTTCGTAAATGATTCGTATGTATGTTTCTTCATGTTTCATTTGTTTAATGTATTATCATTTTCTATATATCTTAATATTTTAAATTTTGTTGCAGCTGTAGAGAAGTCTTTTCCTACTTCTTTGGTATCACCGGATAAGACCACGGGAGATTGCAATAAGGCATTCCTCGAAGATGAACGGGACATGTCAATTAAGACGGGTGAGGTCAATGTTGTTCATGGTAGTGGCAGGAATAGACGTAGTGCCCGGTTGCATAATGTTAAACTCTGCCAGAAGAAACAGCCTGTTACCGGAGATCCGGCAACACATCCAGATAAAGACCAGATCTCCACCTTACCAGCCTTGGAAAAGAGTGATGGAGGACTGGAAAGTCTTATGACACCCATTCAGTCACAAAAGGGAATAGTAAACACATTGTCTGGGCAGGTTACTAATAAATTCAGATCCAGAATTCCCAAAACTTTTTCAGCGAAGACTGGAGGTTGTTATAAGAGAAAAAGGGTGACAGTTGAAACAGAAGCAGTTGGACCTAAGAGAAAAAATATTAAACCCGCATCTTCTGTAAAATCTCCACAAGGAAGGGATACAAGTCACTCGAACAAGGGAAGCACACAGAGATTGTCCTCTGTTTCTCCAGAGTCATTGGGTCTCAAAAAATCAAGATCTGGTAAATTTTCCAATCATTGTCAATTCATGAAGGAACACATCATATTTTGTAATTGATAATATATACACTCTATTTGCCTCTCATGAATTGTCATTCATGTTGTCCAACACTTCTCACCCAATGCCATTGTTAGCATTTTTGTATATGGTAATTTGAAAAGCCATTTACTTCACTCTATTCTTTATGTTTGGGTTATTTTGTAGGAAGGTGGCTTCTACCTCGCTTAGAGTTTTGGCGCAATCAAACACCTATTTATAATATGGTATGTGATATCTTCATTTGATAGTGCTTATTTTTCCCAGCTGCCATTATTAGATATCATATGCATACCATTTTATATCTAGGATTTATTTTCTCTTTAAGGTGGGTAAGATAAACAAAGGGTGAAGATTTACAATCTATAATCTTTTAACTGGTTCTTTTTACATTTGACCACCCAGGACCGCGAGATTATAGAAATTCAGGAAGGCTAGTCTTTTATATCACCTTTTAGAGGCTCTTCACCATCATTGGGCTGGTATATATTTCTTTGTTGAGAAACATTTTCAAGTAAATTTTTTTTATCAGAACTATCATGTGGAATGACATTTTCTTAATTCGTTGAACTATCTCAGCTCAACTAACCTAACTAATTCTCTCTAACCTTGTTACAGGTCTTTAAAAAATGAGCGGAAGAAAAGAGTTTGTACTGAGAGCAAATGCAGAAGTTATAAATAGTGAAGACTGAAGTCTGAAGTGTGCAAAGGGAGATAATTAATTAGGGTAGATTACAACCGAACATACAAATGGTATATGATTAACAGGGCAGAGATTTAGGCATAATGGAGATGTGA
Protein sequence

>MS.gene058998.t1

MNEHRTLENGFTPQISKNFLYGFPPNWESYALECTGEGSEADIDSANAVPDSVSAICQEFFFDGEENFIPTSLVLPDEALGKCKKPFGTKECKASKEMSGVDVTYGSGENRHGTRLRAINVCQRKQQPASGGHRRHPNKKQSSTSTEVETRDSDTAISENVSVNHPEISSDAVEKSFPTSLVSPDKTTGDCNKAFLEDERDMSIKTGEVNVVHGSGRNRRSARLHNVKLCQKKQPVTGDPATHPDKDQISTLPALEKSDGGLESLMTPIQSQKGIVNTLSGQVTNKFRSRIPKTFSAKTGGCYKRKRVTVETEAVGPKRKNIKPASSVKSPQGRDTSHSNKGSTQRLSSVSPESLGLKKSRSGRWLLPRLEFWRNQTPIYNMGRDLGIMEM