AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059085

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059085.t1 MTR_1g009220 94.720 322 14 1 1 322 1 319 0.0 601
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059085.t1 AT4G31270 39.011 182 76 5 10 157 7 187 4.67e-33 124

Find 77 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCACATAGTTGTTTA+AGG 0.160506 1.2:+10712044 None:intergenic
TGTTAGGCTTCAAAAGAAAA+AGG 0.285998 1.2:-10712564 MS.gene059085:intron
TCATCTGGCCAGTATTTCTT+AGG 0.289249 1.2:+10712505 None:intergenic
CCTTCTCACATCTTCGTATT+CGG 0.302599 1.2:+10715341 None:intergenic
AGAGAGAGTGATGGTGAATT+GGG 0.352988 1.2:-10715231 MS.gene059085:CDS
GTAATCTTCATTTACACTCT+TGG 0.360201 1.2:+10712020 None:intergenic
TCATGGTGTTTCTCTACTTC+CGG 0.372520 1.2:+10712310 None:intergenic
TGGCTCAGTGTCGTCGGAAA+TGG 0.376897 1.2:-10715377 MS.gene059085:CDS
GTTATGTTTCCTACACTTGC+CGG 0.378853 1.2:+10712262 None:intergenic
ACTGTTGAAATTGATATGAT+TGG 0.385377 1.2:-10715189 MS.gene059085:CDS
GATTCGGTGAGTGTCCATTC+TGG 0.387850 1.2:+10715519 None:intergenic
AGTTGAAATCTCCTGCAAAA+AGG 0.388370 1.2:-10712369 MS.gene059085:CDS
TTCAGAAGCCTAAGAAATAC+TGG 0.395483 1.2:-10712513 MS.gene059085:CDS
CTTGCCGGCTCTGGCAGCTT+CGG 0.397996 1.2:+10712277 None:intergenic
TTCTCAAAACTGCGCCGCCT+TGG 0.404423 1.2:-10715415 MS.gene059085:CDS
TTTGCAGGAGATTTCAACTT+TGG 0.405070 1.2:+10712373 None:intergenic
CTTGGACGTTGATCGGAACT+TGG 0.405357 1.2:-10715397 MS.gene059085:CDS
TAGAGAGAGTGATGGTGAAT+TGG 0.407373 1.2:-10715232 MS.gene059085:CDS
TTTCCTACACTTGCCGGCTC+TGG 0.409242 1.2:+10712268 None:intergenic
GTTGAGGAGGATGAGGGATT+CGG 0.411008 1.2:+10715503 None:intergenic
CCCTTGGCGTCTCGTAAATT+CGG 0.434296 1.2:+10712105 None:intergenic
AAGATCACATAGTTGTTTAA+GGG 0.437234 1.2:+10712045 None:intergenic
GTGAAAGGGAGAGAAGAAAG+AGG 0.448339 1.2:-10715264 MS.gene059085:CDS
TGCTGAATCCGCAGACTGTA+AGG 0.450242 1.2:-10712161 MS.gene059085:CDS
TGAGAAATTGACGGAACATT+CGG 0.450815 1.2:+10715461 None:intergenic
TCTTCTAAATGATTATTCTC+AGG 0.452633 1.2:+10712427 None:intergenic
AGCGGCATCTCTATGTGCTT+TGG 0.456994 1.2:+10712343 None:intergenic
TTTCTCATCTTACCAACAAT+GGG 0.464013 1.2:-10715445 MS.gene059085:CDS
GAAATTGATATGATTGGAAC+TGG 0.465611 1.2:-10715183 MS.gene059085:intron
TTTCTCTCAACTTTACTTCG+AGG 0.470159 1.2:+10712541 None:intergenic
AAAGCCGAAGCTGCCAGAGC+CGG 0.474090 1.2:-10712281 MS.gene059085:CDS
TGAAAGGGAGAGAAGAAAGA+GGG 0.476638 1.2:-10715263 MS.gene059085:CDS
TTGCTATGAGCTTATCCCCT+TGG 0.480296 1.2:+10712089 None:intergenic
GAGAAATGATGTCCCATTGT+TGG 0.484709 1.2:+10715433 None:intergenic
TGAATTGGGGAATGAGGAAA+GGG 0.493335 1.2:-10715217 MS.gene059085:CDS
ATTTCTCATCTTACCAACAA+TGG 0.500297 1.2:-10715446 MS.gene059085:CDS
GGATAAGCTCATAGCAAGGC+TGG 0.507900 1.2:-10712083 MS.gene059085:CDS
GCGCCGCCTTGGACGTTGAT+CGG 0.508679 1.2:-10715404 MS.gene059085:CDS
CGATTTCGTTGAGGAGGATG+AGG 0.509366 1.2:+10715496 None:intergenic
ACTTCTTCGTGAATTACCTC+AGG 0.522669 1.2:+10712451 None:intergenic
CGGTTTCCGACAGCGTCGCG+TGG 0.523211 1.2:+10715564 None:intergenic
GAAACATAACAAATAACAGC+AGG 0.523213 1.2:-10712249 MS.gene059085:CDS
GTGAATTGGGGAATGAGGAA+AGG 0.524440 1.2:-10715218 MS.gene059085:CDS
GACGGAACATTCGGCTTCGA+CGG 0.534383 1.2:+10715470 None:intergenic
TTGGTAAGATGAGAAATTGA+CGG 0.540766 1.2:+10715452 None:intergenic
GAGAGAGTGATGGTGAATTG+GGG 0.547605 1.2:-10715230 MS.gene059085:CDS
GAAAGGGAGAGAAGAAAGAG+GGG 0.553376 1.2:-10715262 MS.gene059085:CDS
TGAGAAGGAGAAGAAGAGCG+TGG 0.553442 1.2:-10715326 MS.gene059085:CDS
CTGCCAGAGCCGGCAAGTGT+AGG 0.555468 1.2:-10712271 MS.gene059085:CDS
GGAACTTGGCTCAGTGTCGT+CGG 0.559240 1.2:-10715383 MS.gene059085:CDS
CCGAATTTACGAGACGCCAA+GGG 0.559601 1.2:-10712105 MS.gene059085:CDS
TGATGGTGAATTGGGGAATG+AGG 0.564572 1.2:-10715223 MS.gene059085:CDS
GATTTCGTTGAGGAGGATGA+GGG 0.571755 1.2:+10715497 None:intergenic
ACCGAATTTACGAGACGCCA+AGG 0.572193 1.2:-10712106 MS.gene059085:CDS
GATCACAAGCAGCACCAGAA+TGG 0.572292 1.2:-10715533 MS.gene059085:CDS
GAAGTTGATAGAGAGAGTGA+TGG 0.574740 1.2:-10715240 MS.gene059085:CDS
CTGTTGAGAGAGTTGTGAAA+GGG 0.578505 1.2:-10715278 MS.gene059085:CDS
CTGCTTGTGATCGTGTGCGT+CGG 0.579022 1.2:+10715544 None:intergenic
AGCTAACACAGAGAATATAG+AGG 0.579463 1.2:-10712137 MS.gene059085:CDS
CGACGGCGGCGATTTCGTTG+AGG 0.581332 1.2:+10715487 None:intergenic
GAAAACCCACGCGACGCTGT+CGG 0.582372 1.2:-10715570 MS.gene059085:CDS
GGAACATTCGGCTTCGACGG+CGG 0.584041 1.2:+10715473 None:intergenic
CAAGTTCCGATCAACGTCCA+AGG 0.590355 1.2:+10715398 None:intergenic
GGTTTCCGACAGCGTCGCGT+GGG 0.599447 1.2:+10715565 None:intergenic
GATAAGCTCATAGCAAGGCT+GGG 0.602989 1.2:-10712082 MS.gene059085:CDS
GCTGTTGAGAGAGTTGTGAA+AGG 0.604033 1.2:-10715279 MS.gene059085:CDS
CGGCGGCGATTTCGTTGAGG+AGG 0.604221 1.2:+10715490 None:intergenic
GTTAGCTTCCTTACAGTCTG+CGG 0.610667 1.2:+10712153 None:intergenic
CCGAATACGAAGATGTGAGA+AGG 0.613703 1.2:-10715341 MS.gene059085:CDS
GATGCCGCTGCAGAATCTAC+CGG 0.617116 1.2:-10712329 MS.gene059085:CDS
TAACAGCAGGGAAGAAAACG+AGG 0.617815 1.2:-10712236 MS.gene059085:CDS
GGCGTCTCGTAAATTCGGTA+CGG 0.624328 1.2:+10712110 None:intergenic
AAACATAACAAATAACAGCA+GGG 0.628611 1.2:-10712248 MS.gene059085:CDS
CGAATTTACGAGACGCCAAG+GGG 0.658164 1.2:-10712104 MS.gene059085:CDS
AAGGGGATAAGCTCATAGCA+AGG 0.667108 1.2:-10712087 MS.gene059085:CDS
ACTTCCGGTAGATTCTGCAG+CGG 0.711910 1.2:+10712325 None:intergenic
GTTCCGATCAACGTCCAAGG+CGG 0.725968 1.2:+10715401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATTATAATTTAAGCT+AGG - chr1.2:10713267-10713286 MS.gene059085:intron 15.0%
!!! AAAATTGATTTTGATATGTT+TGG + chr1.2:10712763-10712782 None:intergenic 15.0%
!!! CAAAAAAATCTACTTTTTAT+AGG - chr1.2:10714497-10714516 MS.gene059085:intron 15.0%
!!! TCATTTGATTTATTGTTTTA+AGG - chr1.2:10713965-10713984 MS.gene059085:intron 15.0%
!! AAACAATTAGGCTATATTTA+TGG + chr1.2:10714240-10714259 None:intergenic 20.0%
!! ATTTGTGTGAATTAATTGTT+AGG - chr1.2:10715023-10715042 MS.gene059085:intron 20.0%
!! GTATAAGATAAAATCTATCT+TGG + chr1.2:10712911-10712930 None:intergenic 20.0%
!! TAAAATTAACTTTGCATGTT+TGG + chr1.2:10712729-10712748 None:intergenic 20.0%
!! TTTAGTTAAACATGAGTTTA+AGG + chr1.2:10713386-10713405 None:intergenic 20.0%
!!! AACTATTTCAAGTGTATTTT+TGG + chr1.2:10712825-10712844 None:intergenic 20.0%
!!! GATTTTGAGTTAATTGATTA+TGG - chr1.2:10712538-10712557 MS.gene059085:CDS 20.0%
!!! GTAGATTTTTTTGTAGAATA+TGG + chr1.2:10714490-10714509 None:intergenic 20.0%
!!! TCATATTTTAAGAGTGAATA+TGG + chr1.2:10713181-10713200 None:intergenic 20.0%
!!! TGAAGACATAAAATTGATTT+TGG - chr1.2:10713221-10713240 MS.gene059085:intron 20.0%
!!! TTTTATTCCAATTTTGTTCT+AGG - chr1.2:10714011-10714030 MS.gene059085:intron 20.0%
! AAACACTTTAAATGAAATCG+AGG - chr1.2:10714424-10714443 MS.gene059085:intron 25.0%
! AAACATAACAAATAACAGCA+GGG - chr1.2:10715355-10715374 MS.gene059085:CDS 25.0%
! AAGATCACATAGTTGTTTAA+GGG + chr1.2:10715561-10715580 None:intergenic 25.0%
! ACTGTTGAAATTGATATGAT+TGG - chr1.2:10712414-10712433 MS.gene059085:CDS 25.0%
! AGAAAATCTCAAAGAAAAAC+AGG - chr1.2:10715127-10715146 MS.gene059085:intron 25.0%
! AGTGTCAATTATAACTGAAA+CGG + chr1.2:10714565-10714584 None:intergenic 25.0%
! CTAGTAGCATTAAAATTTCA+TGG - chr1.2:10714177-10714196 MS.gene059085:intron 25.0%
! CTCAGAATTCTAATTATTGA+GGG - chr1.2:10714930-10714949 MS.gene059085:intron 25.0%
! GAATATAAGTAGGTAGATTT+AGG - chr1.2:10713756-10713775 MS.gene059085:intron 25.0%
! GAGAATAAAATGCCATAATA+AGG - chr1.2:10713657-10713676 MS.gene059085:intron 25.0%
! TCTCAGAATTCTAATTATTG+AGG - chr1.2:10714929-10714948 MS.gene059085:intron 25.0%
! TCTTCTAAATGATTATTCTC+AGG + chr1.2:10715179-10715198 None:intergenic 25.0%
! TGAGGAAAAATTAATCTTAC+TGG - chr1.2:10712696-10712715 MS.gene059085:intron 25.0%
! TGTCAATTAGCAATTGTATT+AGG + chr1.2:10714462-10714481 None:intergenic 25.0%
! TTCACTCTTAAAATATGACT+TGG - chr1.2:10713183-10713202 MS.gene059085:intron 25.0%
! TTGAAAAATTCTAAGCAGTA+AGG + chr1.2:10714996-10715015 None:intergenic 25.0%
! TTGCATACTAATCCTTATTA+TGG + chr1.2:10713672-10713691 None:intergenic 25.0%
! TTTATTGTGTTTGATATGAC+TGG - chr1.2:10714325-10714344 MS.gene059085:intron 25.0%
!!! AGATCAATTCTAGAATCAAA+AGG - chr1.2:10712644-10712663 MS.gene059085:intron 25.0%
!!! ATTCGTTTTTCTCTATTGTT+AGG - chr1.2:10712451-10712470 MS.gene059085:CDS 25.0%
!!! GCTTTTAGTTTGAATTAATG+AGG - chr1.2:10712678-10712697 MS.gene059085:intron 25.0%
!!! TTTTCTTTGAGATTTTCTGA+AGG + chr1.2:10715125-10715144 None:intergenic 25.0%
AAAGTGACATGATACTATGA+AGG - chr1.2:10713917-10713936 MS.gene059085:intron 30.0%
AAATAGTTGTGAGACTGTTT+GGG - chr1.2:10712837-10712856 MS.gene059085:intron 30.0%
AAGTGACATGATACTATGAA+GGG - chr1.2:10713918-10713937 MS.gene059085:intron 30.0%
ACAAATGATAGTTACTAGCT+AGG - chr1.2:10713091-10713110 MS.gene059085:intron 30.0%
ACACAATAAAGCCAGATTTA+CGG + chr1.2:10714315-10714334 None:intergenic 30.0%
ATACAACTAAGAAGACTAGA+CGG + chr1.2:10714717-10714736 None:intergenic 30.0%
ATAGCATACTGACAAAACAT+TGG + chr1.2:10714760-10714779 None:intergenic 30.0%
ATTTCTCATCTTACCAACAA+TGG - chr1.2:10712157-10712176 MS.gene059085:CDS 30.0%
CAATGCTAACACAAACAATT+AGG + chr1.2:10714252-10714271 None:intergenic 30.0%
CAATTAGCAATTGTATTAGG+AGG + chr1.2:10714459-10714478 None:intergenic 30.0%
GAAACATAACAAATAACAGC+AGG - chr1.2:10715354-10715373 MS.gene059085:CDS 30.0%
GAAATTGATATGATTGGAAC+TGG - chr1.2:10712420-10712439 MS.gene059085:CDS 30.0%
GAAGATCACATAGTTGTTTA+AGG + chr1.2:10715562-10715581 None:intergenic 30.0%
GACTAGAATAGAAAACTATG+TGG - chr1.2:10713633-10713652 MS.gene059085:intron 30.0%
GAGATGTCAATAAATACTGA+TGG - chr1.2:10713702-10713721 MS.gene059085:intron 30.0%
GAGGTAACGTGATTTATATT+TGG - chr1.2:10712577-10712596 MS.gene059085:intron 30.0%
GTACAGAGATACTCATAATT+TGG + chr1.2:10714152-10714171 None:intergenic 30.0%
GTGTCAATTATAACTGAAAC+GGG + chr1.2:10714564-10714583 None:intergenic 30.0%
TAGCATACTGACAAAACATT+GGG + chr1.2:10714759-10714778 None:intergenic 30.0%
TATCAGTGTTAGCATTTCAT+GGG - chr1.2:10714662-10714681 MS.gene059085:intron 30.0%
TGTCAATAAATACTGATGGA+TGG - chr1.2:10713706-10713725 MS.gene059085:intron 30.0%
TGTTAGGCTTCAAAAGAAAA+AGG - chr1.2:10715039-10715058 MS.gene059085:intron 30.0%
TTGGTAAGATGAGAAATTGA+CGG + chr1.2:10712154-10712173 None:intergenic 30.0%
TTTCTCATCTTACCAACAAT+GGG - chr1.2:10712158-10712177 MS.gene059085:CDS 30.0%
! AAGCTAGGATTTGTATCTTT+TGG - chr1.2:10713282-10713301 MS.gene059085:intron 30.0%
! ATGTTTTGTCAGTATGCTAT+AGG - chr1.2:10714760-10714779 MS.gene059085:intron 30.0%
! TACACGTCTATAATCACTTT+TGG - chr1.2:10712782-10712801 MS.gene059085:intron 30.0%
! TGTTTGTGTTAGCATTGAAT+GGG - chr1.2:10714255-10714274 MS.gene059085:intron 30.0%
! TTGTTTGTGTTAGCATTGAA+TGG - chr1.2:10714254-10714273 MS.gene059085:intron 30.0%
!! TCAACTTTGGTTTCTTTTCT+AGG + chr1.2:10715220-10715239 None:intergenic 30.0%
!! TGAACTTGTTTATCCTTTTG+TGG - chr1.2:10714214-10714233 MS.gene059085:intron 30.0%
!!! TTTGTGCATGTTTGGTTTTA+CGG - chr1.2:10712504-10712523 MS.gene059085:CDS 30.0%
AACGAGACCTAGAACAAAAT+TGG + chr1.2:10714021-10714040 None:intergenic 35.0%
AATAGTTGTGAGACTGTTTG+GGG - chr1.2:10712838-10712857 MS.gene059085:intron 35.0%
AATATGGTGCTTCTTCTAAC+AGG + chr1.2:10713165-10713184 None:intergenic 35.0%
AGCTAACACAGAGAATATAG+AGG - chr1.2:10715466-10715485 MS.gene059085:CDS 35.0%
AGTTGAAATCTCCTGCAAAA+AGG - chr1.2:10715234-10715253 MS.gene059085:CDS 35.0%
CAGAGAATTTACAGGAACAA+TGG - chr1.2:10714351-10714370 MS.gene059085:intron 35.0%
CATTGTTCCTGTAAATTCTC+TGG + chr1.2:10714353-10714372 None:intergenic 35.0%
CTACCAAGTGGAATATAAGT+AGG - chr1.2:10713746-10713765 MS.gene059085:intron 35.0%
CTACCTACTTATATTCCACT+TGG + chr1.2:10713752-10713771 None:intergenic 35.0%
CTACTTATATTCCACTTGGT+AGG + chr1.2:10713748-10713767 None:intergenic 35.0%
CTATCAGTGTTAGCATTTCA+TGG - chr1.2:10714661-10714680 MS.gene059085:intron 35.0%
GAAATAGTTGTGAGACTGTT+TGG - chr1.2:10712836-10712855 MS.gene059085:intron 35.0%
GCTATATTTATGGCCACAAA+AGG + chr1.2:10714230-10714249 None:intergenic 35.0%
TAAGGAGCTTCTTTATCTAG+CGG - chr1.2:10713983-10714002 MS.gene059085:intron 35.0%
TATAGTACTAAACACGCCTA+TGG - chr1.2:10713450-10713469 MS.gene059085:intron 35.0%
TCACAACGAGTTCAAATTCT+AGG + chr1.2:10713813-10713832 None:intergenic 35.0%
TGAGAAATTGACGGAACATT+CGG + chr1.2:10712145-10712164 None:intergenic 35.0%
TTCAGAAGCCTAAGAAATAC+TGG - chr1.2:10715090-10715109 MS.gene059085:intron 35.0%
TTTCTCTCAACTTTACTTCG+AGG + chr1.2:10715065-10715084 None:intergenic 35.0%
TTTGCAGGAGATTTCAACTT+TGG + chr1.2:10715233-10715252 None:intergenic 35.0%
! AAGGATTAGTATGCAAGTTG+TGG - chr1.2:10713676-10713695 MS.gene059085:intron 35.0%
! CTACATAGTTTGTGCATGTT+TGG - chr1.2:10712496-10712515 MS.gene059085:CDS 35.0%
!! AACAGTCTCTTTTCTAGTGA+GGG + chr1.2:10713790-10713809 None:intergenic 35.0%
AAACTCCCTCTTTCTACCAT+AGG + chr1.2:10713469-10713488 None:intergenic 40.0%
AATGAAGACCTATACAGCAC+AGG + chr1.2:10714393-10714412 None:intergenic 40.0%
ACAGGAACAATGGCACATTA+AGG - chr1.2:10714361-10714380 MS.gene059085:intron 40.0%
ACTAGCTAGGCATAGAGAAA+GGG - chr1.2:10713104-10713123 MS.gene059085:intron 40.0%
ACTGGAACCAGAGAATTTAC+AGG - chr1.2:10714343-10714362 MS.gene059085:intron 40.0%
ACTTCTTCGTGAATTACCTC+AGG + chr1.2:10715155-10715174 None:intergenic 40.0%
AGTGTTAGCATTTCATGGGA+AGG - chr1.2:10714666-10714685 MS.gene059085:intron 40.0%
ATACTATGAAGGGTTCGAAG+GGG - chr1.2:10713928-10713947 MS.gene059085:intron 40.0%
ATAGTTGTGAGACTGTTTGG+GGG - chr1.2:10712839-10712858 MS.gene059085:intron 40.0%
ATGAAGACCTATACAGCACA+GGG + chr1.2:10714392-10714411 None:intergenic 40.0%
CAAAGAAAAACAGGATCCTG+AGG - chr1.2:10715136-10715155 MS.gene059085:intron 40.0%
CCTTCTCACATCTTCGTATT+CGG + chr1.2:10712265-10712284 None:intergenic 40.0%
CTGCAAAACCATTAGATTCG+GGG + chr1.2:10713540-10713559 None:intergenic 40.0%
CTGTCAATCAAGTGTACGTA+AGG + chr1.2:10714627-10714646 None:intergenic 40.0%
CTGTTGAGAGAGTTGTGAAA+GGG - chr1.2:10712325-10712344 MS.gene059085:CDS 40.0%
CTTACGTACACTTGATTGAC+AGG - chr1.2:10714625-10714644 MS.gene059085:intron 40.0%
GAGAAATGATGTCCCATTGT+TGG + chr1.2:10712173-10712192 None:intergenic 40.0%
GATACTATGAAGGGTTCGAA+GGG - chr1.2:10713927-10713946 MS.gene059085:intron 40.0%
GCTGCAAAACCATTAGATTC+GGG + chr1.2:10713541-10713560 None:intergenic 40.0%
GGAGTTTGGATTCTTTGTAG+CGG - chr1.2:10713482-10713501 MS.gene059085:intron 40.0%
GGCTGCAAAACCATTAGATT+CGG + chr1.2:10713542-10713561 None:intergenic 40.0%
GTTATGTTTCCTACACTTGC+CGG + chr1.2:10715344-10715363 None:intergenic 40.0%
TACTAGCTAGGCATAGAGAA+AGG - chr1.2:10713103-10713122 MS.gene059085:intron 40.0%
TATGGTAGAAAGAGGGAGTT+TGG - chr1.2:10713468-10713487 MS.gene059085:intron 40.0%
TCAATCAAGTGTACGTAAGG+AGG + chr1.2:10714624-10714643 None:intergenic 40.0%
TCATCTGGCCAGTATTTCTT+AGG + chr1.2:10715101-10715120 None:intergenic 40.0%
TCATGGTGTTTCTCTACTTC+CGG + chr1.2:10715296-10715315 None:intergenic 40.0%
TGAAAGGGAGAGAAGAAAGA+GGG - chr1.2:10712340-10712359 MS.gene059085:CDS 40.0%
TGAATTGGGGAATGAGGAAA+GGG - chr1.2:10712386-10712405 MS.gene059085:CDS 40.0%
TGATACTATGAAGGGTTCGA+AGG - chr1.2:10713926-10713945 MS.gene059085:intron 40.0%
! AAATACTGATGGATGGTCCA+GGG - chr1.2:10713713-10713732 MS.gene059085:intron 40.0%
! AGAGAGAGTGATGGTGAATT+GGG - chr1.2:10712372-10712391 MS.gene059085:CDS 40.0%
! GCTTCGGCTTTTCATTATCA+TGG + chr1.2:10715313-10715332 None:intergenic 40.0%
! TAAATACTGATGGATGGTCC+AGG - chr1.2:10713712-10713731 MS.gene059085:intron 40.0%
! TAGAGAGAGTGATGGTGAAT+TGG - chr1.2:10712371-10712390 MS.gene059085:CDS 40.0%
! TCCTTTTGTGACCGTAAATC+TGG - chr1.2:10714301-10714320 MS.gene059085:intron 40.0%
! TGGCTAAAAGTGTGTTGAAG+AGG - chr1.2:10712558-10712577 MS.gene059085:CDS 40.0%
! TGTGTTAGCATTGAATGGGA+AGG - chr1.2:10714259-10714278 MS.gene059085:intron 40.0%
!! AATATGACTTGGTGCGTGTT+TGG - chr1.2:10713194-10713213 MS.gene059085:intron 40.0%
!! AGATTTTCTGAAGGCTCATC+TGG + chr1.2:10715116-10715135 None:intergenic 40.0%
!! GAACAGTCTCTTTTCTAGTG+AGG + chr1.2:10713791-10713810 None:intergenic 40.0%
!! GAAGTTGATAGAGAGAGTGA+TGG - chr1.2:10712363-10712382 MS.gene059085:CDS 40.0%
!!! AATCACTTTTGGCTCCTCTA+GGG - chr1.2:10712793-10712812 MS.gene059085:intron 40.0%
!!! ATTTTTGGTTTCACCCCTAG+AGG + chr1.2:10712810-10712829 None:intergenic 40.0%
!!! TAATCACTTTTGGCTCCTCT+AGG - chr1.2:10712792-10712811 MS.gene059085:intron 40.0%
AACACGCCTATGGTAGAAAG+AGG - chr1.2:10713460-10713479 MS.gene059085:intron 45.0%
AACCATTGTTCCCCTTGTTG+TGG + chr1.2:10713137-10713156 None:intergenic 45.0%
AAGGGAGTAGTTCCACAACA+AGG - chr1.2:10713122-10713141 MS.gene059085:intron 45.0%
AAGGGGATAAGCTCATAGCA+AGG - chr1.2:10715516-10715535 MS.gene059085:CDS 45.0%
ACAACAAGGGGAACAATGGT+TGG - chr1.2:10713136-10713155 MS.gene059085:intron 45.0%
ACACGCCTATGGTAGAAAGA+GGG - chr1.2:10713461-10713480 MS.gene059085:intron 45.0%
ACGTACACTTGATTGACAGG+AGG - chr1.2:10714628-10714647 MS.gene059085:intron 45.0%
ACTGATTGCCCCGAATCTAA+TGG - chr1.2:10713529-10713548 MS.gene059085:intron 45.0%
AGCATTTCATGGGAAGGTTG+AGG - chr1.2:10714672-10714691 MS.gene059085:intron 45.0%
AGGGAGTAGTTCCACAACAA+GGG - chr1.2:10713123-10713142 MS.gene059085:intron 45.0%
CAGCAACTACACAAGTGCAA+CGG + chr1.2:10713563-10713582 None:intergenic 45.0%
CCGAATACGAAGATGTGAGA+AGG - chr1.2:10712262-10712281 MS.gene059085:CDS 45.0%
CTATGATCGCGTCATCTGAT+AGG + chr1.2:10713039-10713058 None:intergenic 45.0%
GAAAGGGAGAGAAGAAAGAG+GGG - chr1.2:10712341-10712360 MS.gene059085:CDS 45.0%
GATAAGCTCATAGCAAGGCT+GGG - chr1.2:10715521-10715540 MS.gene059085:CDS 45.0%
GATTTCGTTGAGGAGGATGA+GGG + chr1.2:10712109-10712128 None:intergenic 45.0%
GCCAGATTTACGGTCACAAA+AGG + chr1.2:10714305-10714324 None:intergenic 45.0%
GCTGTTGAGAGAGTTGTGAA+AGG - chr1.2:10712324-10712343 MS.gene059085:CDS 45.0%
GGAATATCCCTGTGCTGTAT+AGG - chr1.2:10714382-10714401 MS.gene059085:intron 45.0%
GTGAAAGGGAGAGAAGAAAG+AGG - chr1.2:10712339-10712358 MS.gene059085:CDS 45.0%
GTGAATTGGGGAATGAGGAA+AGG - chr1.2:10712385-10712404 MS.gene059085:CDS 45.0%
TAACAGCAGGGAAGAAAACG+AGG - chr1.2:10715367-10715386 MS.gene059085:CDS 45.0%
TAGTCACGAGGGTAGTTAGT+GGG + chr1.2:10714536-10714555 None:intergenic 45.0%
TAGTTGCTGCACAGGTATGT+GGG - chr1.2:10713574-10713593 MS.gene059085:intron 45.0%
TCAAGTGTACGTAAGGAGGA+AGG + chr1.2:10714620-10714639 None:intergenic 45.0%
TTAGTCACGAGGGTAGTTAG+TGG + chr1.2:10714537-10714556 None:intergenic 45.0%
TTCCACAACAAGGGGAACAA+TGG - chr1.2:10713132-10713151 MS.gene059085:intron 45.0%
TTGCTATGAGCTTATCCCCT+TGG + chr1.2:10715517-10715536 None:intergenic 45.0%
! AGCATTGAATGGGAAGGTTG+AGG - chr1.2:10714265-10714284 MS.gene059085:intron 45.0%
! GAGAGAGTGATGGTGAATTG+GGG - chr1.2:10712373-10712392 MS.gene059085:CDS 45.0%
! GGTCTCGTTTTGTCACACAT+TGG - chr1.2:10714032-10714051 MS.gene059085:intron 45.0%
! GTTAGCTTCCTTACAGTCTG+CGG + chr1.2:10715453-10715472 None:intergenic 45.0%
! TGATGGTGAATTGGGGAATG+AGG - chr1.2:10712380-10712399 MS.gene059085:CDS 45.0%
!! AAACGGGTTAGTTAGTCACG+AGG + chr1.2:10714548-10714567 None:intergenic 45.0%
!! AACGGGTTAGTTAGTCACGA+GGG + chr1.2:10714547-10714566 None:intergenic 45.0%
!! TCTCTTTTCTAGTGAGGGTG+AGG + chr1.2:10713785-10713804 None:intergenic 45.0%
!!! ATCACTTTTGGCTCCTCTAG+GGG - chr1.2:10712794-10712813 MS.gene059085:intron 45.0%
!!! TTTTGTAAAAATAATAATAT+TGG + chr1.2:10714848-10714867 None:intergenic 5.0%
ACCGAATTTACGAGACGCCA+AGG - chr1.2:10715497-10715516 MS.gene059085:CDS 50.0%
ACTCGTTGTGAGTGACCTTC+AGG - chr1.2:10713822-10713841 MS.gene059085:intron 50.0%
ACTTCCGGTAGATTCTGCAG+CGG + chr1.2:10715281-10715300 None:intergenic 50.0%
AGCGGCATCTCTATGTGCTT+TGG + chr1.2:10715263-10715282 None:intergenic 50.0%
CAAGTTCCGATCAACGTCCA+AGG + chr1.2:10712208-10712227 None:intergenic 50.0%
CACTTGTGTAGTTGCTGCAC+AGG - chr1.2:10713566-10713585 MS.gene059085:intron 50.0%
CCCTTGGCGTCTCGTAAATT+CGG + chr1.2:10715501-10715520 None:intergenic 50.0%
CCGAATTTACGAGACGCCAA+GGG - chr1.2:10715498-10715517 MS.gene059085:CDS 50.0%
CGAATTTACGAGACGCCAAG+GGG - chr1.2:10715499-10715518 MS.gene059085:CDS 50.0%
CGATTTCGTTGAGGAGGATG+AGG + chr1.2:10712110-10712129 None:intergenic 50.0%
CTTGGACGTTGATCGGAACT+TGG - chr1.2:10712206-10712225 MS.gene059085:CDS 50.0%
GATCACAAGCAGCACCAGAA+TGG - chr1.2:10712070-10712089 MS.gene059085:CDS 50.0%
GCTGCAGAGTTGTTGGATTC+AGG - chr1.2:10713504-10713523 MS.gene059085:intron 50.0%
GGATAAGCTCATAGCAAGGC+TGG - chr1.2:10715520-10715539 MS.gene059085:CDS 50.0%
GGCGTCTCGTAAATTCGGTA+CGG + chr1.2:10715496-10715515 None:intergenic 50.0%
GGGAGTAGTTCCACAACAAG+GGG - chr1.2:10713124-10713143 MS.gene059085:intron 50.0%
GTAGTTGCTGCACAGGTATG+TGG - chr1.2:10713573-10713592 MS.gene059085:intron 50.0%
GTTGAGGAGGATGAGGGATT+CGG + chr1.2:10712103-10712122 None:intergenic 50.0%
GTTGTCGTCTTGCAACCTGA+AGG + chr1.2:10713840-10713859 None:intergenic 50.0%
TGAGAAGGAGAAGAAGAGCG+TGG - chr1.2:10712277-10712296 MS.gene059085:CDS 50.0%
TGCTGAATCCGCAGACTGTA+AGG - chr1.2:10715442-10715461 MS.gene059085:CDS 50.0%
!! GATTCGGTGAGTGTCCATTC+TGG + chr1.2:10712087-10712106 None:intergenic 50.0%
!! TGCTTTGGACGCCTTTTTGC+AGG + chr1.2:10715248-10715267 None:intergenic 50.0%
CTAGACGGCTAGACGCATAG+TGG + chr1.2:10714702-10714721 None:intergenic 55.0%
CTGCTTGTGATCGTGTGCGT+CGG + chr1.2:10712062-10712081 None:intergenic 55.0%
GACGGAACATTCGGCTTCGA+CGG + chr1.2:10712136-10712155 None:intergenic 55.0%
GATGCCGCTGCAGAATCTAC+CGG - chr1.2:10715274-10715293 MS.gene059085:CDS 55.0%
GGAACTTGGCTCAGTGTCGT+CGG - chr1.2:10712220-10712239 MS.gene059085:CDS 55.0%
GTTCCGATCAACGTCCAAGG+CGG + chr1.2:10712205-10712224 None:intergenic 55.0%
TGGCTCAGTGTCGTCGGAAA+TGG - chr1.2:10712226-10712245 MS.gene059085:CDS 55.0%
TGTAGCGGCTGCAGAGTTGT+TGG - chr1.2:10713497-10713516 MS.gene059085:intron 55.0%
TTCTCAAAACTGCGCCGCCT+TGG - chr1.2:10712188-10712207 MS.gene059085:CDS 55.0%
!! TTTCCTACACTTGCCGGCTC+TGG + chr1.2:10715338-10715357 None:intergenic 55.0%
AAAGCCGAAGCTGCCAGAGC+CGG - chr1.2:10715322-10715341 MS.gene059085:CDS 60.0%
GAAAACCCACGCGACGCTGT+CGG - chr1.2:10712033-10712052 MS.gene059085:CDS 60.0%
GGAACATTCGGCTTCGACGG+CGG + chr1.2:10712133-10712152 None:intergenic 60.0%
GGCACGAGACTCCTACCAAG+TGG - chr1.2:10713734-10713753 MS.gene059085:intron 60.0%
TTGGTAGGAGTCTCGTGCCC+TGG + chr1.2:10713733-10713752 None:intergenic 60.0%
CGACGGCGGCGATTTCGTTG+AGG + chr1.2:10712119-10712138 None:intergenic 65.0%
CGGCGGCGATTTCGTTGAGG+AGG + chr1.2:10712116-10712135 None:intergenic 65.0%
CTGCCAGAGCCGGCAAGTGT+AGG - chr1.2:10715332-10715351 MS.gene059085:CDS 65.0%
GCGCCGCCTTGGACGTTGAT+CGG - chr1.2:10712199-10712218 MS.gene059085:CDS 65.0%
GGTTTCCGACAGCGTCGCGT+GGG + chr1.2:10712041-10712060 None:intergenic 65.0%
!! CTTGCCGGCTCTGGCAGCTT+CGG + chr1.2:10715329-10715348 None:intergenic 65.0%
CGGTTTCCGACAGCGTCGCG+TGG + chr1.2:10712042-10712061 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 10712027 10715598 10712027 ID=MS.gene059085
chr1.2 mRNA 10712027 10715598 10712027 ID=MS.gene059085.t1;Parent=MS.gene059085
chr1.2 exon 10715184 10715598 10715184 ID=MS.gene059085.t1.exon1;Parent=MS.gene059085.t1
chr1.2 CDS 10715184 10715598 10715184 ID=cds.MS.gene059085.t1;Parent=MS.gene059085.t1
chr1.2 exon 10712027 10712580 10712027 ID=MS.gene059085.t1.exon2;Parent=MS.gene059085.t1
chr1.2 CDS 10712027 10712580 10712027 ID=cds.MS.gene059085.t1;Parent=MS.gene059085.t1
Gene Sequence

>MS.gene059085

ATGACGGAAAACCCACGCGACGCTGTCGGAAACCGACGCACACGATCACAAGCAGCACCAGAATGGACACTCACCGAATCCCTCATCCTCCTCAACGAAATCGCCGCCGTCGAAGCCGAATGTTCCGTCAATTTCTCATCTTACCAACAATGGGACATCATTTCTCAAAACTGCGCCGCCTTGGACGTTGATCGGAACTTGGCTCAGTGTCGTCGGAAATGGCGTTCGCTACTTGCCGAATACGAAGATGTGAGAAGGAGAAGAAGAGCGTGGAATTCAGATCGTGAATTGTTTGATGCTGTTGAGAGAGTTGTGAAAGGGAGAGAAGAAAGAGGGGAAGTTGATAGAGAGAGTGATGGTGAATTGGGGAATGAGGAAAGGGATGTTACTGTTGAAATTGATATGATTGGAACTGGTATGTATGATTCGTTTTTCTCTATTGTTAGGTTAAATCTACATTTTGTTTTTACTACATAGTTTGTGCATGTTTGGTTTTACGGTGACAAAAATTGATTTTGAGTTAATTGATTATGGCTAAAAGTGTGTTGAAGAGGTAACGTGATTTATATTTGGATGCATTTATGTAAAAGTGAGTTAAACAATGCATTTAAGTGTAAAGATCAATTCTAGAATCAAAAGGTGCAATTTTTAGCTTTTAGTTTGAATTAATGAGGAAAAATTAATCTTACTGGAGAGCAACCAAACATGCAAAGTTAATTTTACTAGAGAGCAACCAAACATATCAAAATCAATTTTACACGTCTATAATCACTTTTGGCTCCTCTAGGGGTGAAACCAAAAATACACTTGAAATAGTTGTGAGACTGTTTGGGGGAACTCATGAAAATAGCTTATGTTTTCAGCTTATTTTCATAAATTCTCCAAGATAGATTTTATCTTATACAAAAATAATTTAACTTTGTTTTATATTTTGTTATTAAAATAGCTTATACATAAACACTTTTTATGATAAACGTTTATGCTAGAATCTGCAAATTAAGTTGCTTATCCTATCAGATGACGCGATCATAGTTAAGCTAGTTGTGTTATCTAGTGTGAGACTAACAAATGATAGTTACTAGCTAGGCATAGAGAAAGGGAGTAGTTCCACAACAAGGGGAACAATGGTTGGAATCCTGTTAGAAGAAGCACCATATTCACTCTTAAAATATGACTTGGTGCGTGTTTGGATTCTGAAGACATAAAATTGATTTTGGTATGTTTGAATGTTCTTGATTAGAATTGATTATAATTTAAGCTAGGATTTGTATCTTTTGGCTCTGAACGTGATCTTTACACTCAAATTTATTGTTGAACTCACTTTCACGTGAATGTATCTAAACATAAAATATTTTACCTTAAACTCATGTTTAACTAAAATCAATTTTAGAAAATTAATTCAATGAAAATCAATTTTCGCTAATATAGTACTAAACACGCCTATGGTAGAAAGAGGGAGTTTGGATTCTTTGTAGCGGCTGCAGAGTTGTTGGATTCAGGTGACTGATTGCCCCGAATCTAATGGTTTTGCAGCCGTTGCACTTGTGTAGTTGCTGCACAGGTATGTGGGTTTAACAGAAAGAGTATGATGCAAGACAATATGCTAGACTAGAATAGAAAACTATGTGGAGAGAATAAAATGCCATAATAAGGATTAGTATGCAAGTTGTGGTTTGAGATGTCAATAAATACTGATGGATGGTCCAGGGCACGAGACTCCTACCAAGTGGAATATAAGTAGGTAGATTTAGGAATCCTCACCCTCACTAGAAAAGAGACTGTTCCTAGAATTTGAACTCGTTGTGAGTGACCTTCAGGTTGCAAGACGACAACAAGCTGCTTTATCAGTATCAATAAATACTAATACATTTGAAACAAATCAAAGTTTTAAAAGTGACATGATACTATGAAGGGTTCGAAGGGGCTAAGAAAAAGTTATCATTTGATTTATTGTTTTAAGGAGCTTCTTTATCTAGCGGTTTAATTTTATTCCAATTTTGTTCTAGGTCTCGTTTTGTCACACATTGGTGTGATGTTAGCTGCAATTGTTGTGATTTATGAATGTGATAATATATTCTTATCATGCAATGCATTTTAAACATAAATGTTGAGTTTCTATCATCCAAATTATGAGTATCTCTGTACTTTGACTAGTAGCATTAAAATTTCATGGTATGATTAACTTGTTGAACTTGTTTATCCTTTTGTGGCCATAAATATAGCCTAATTGTTTGTGTTAGCATTGAATGGGAAGGTTGAGGTTTTCTTCTATTATCCTTTTGTGACCGTAAATCTGGCTTTATTGTGTTTGATATGACTGGAACCAGAGAATTTACAGGAACAATGGCACATTAAGGAATATCCCTGTGCTGTATAGGTCTTCATTGAATTTCAATGAAACACTTTAAATGAAATCGAGGAACTAGCAACCTCCTAATACAATTGCTAATTGACAAAATTCCATATTCTACAAAAAAATCTACTTTTTATAGGATTGCAGATTACTCCCACTAACTACCCTCGTGACTAACTAACCCGTTTCAGTTATAATTGACACTTACAACTTCTCTACTTTTTTTTGCTTTGTCTGCCTTCCTCCTTACGTACACTTGATTGACAGGAGGTCTTTGTGTTCTATCAGTGTTAGCATTTCATGGGAAGGTTGAGGCTTTCCACTATGCGTCTAGCCGTCTAGTCTTCTTAGTTGTATTTCTTCAAACTTTGTATATCCCAATGTTTTGTCAGTATGCTATAGGCTAACTTAATGTATTCTTGATCTCTAAAGAGCACACAAATATTTGCTCAACACGATATAGTTCCAATATTATTATTTTTACAAAATAAAATCACTGCATTTTATTCATAGTAGTACAATTTGAAATAGTTCTATTTGTTACTTCTTTCTCAGAATTCTAATTATTGAGGGCATGAAACTATGTTGTAACTTGTATGTCATGTCATTATCTCCTTACTGCTTAGAATTTTTCAACTAATGCATTTGTGTGAATTAATTGTTAGGCTTCAAAAGAAAAAGGCCTCGAAGTAAAGTTGAGAGAAATTCTGTTCAGAAGCCTAAGAAATACTGGCCAGATGAGCCTTCAGAAAATCTCAAAGAAAAACAGGATCCTGAGGTAATTCACGAAGAAGTGCCTGAGAATAATCATTTAGAAGAAGTGTTTTTGAAAGACTTCCTAGAAAAGAAACCAAAGTTGAAATCTCCTGCAAAAAGGCGTCCAAAGCACATAGAGATGCCGCTGCAGAATCTACCGGAAGTAGAGAAACACCATGATAATGAAAAGCCGAAGCTGCCAGAGCCGGCAAGTGTAGGAAACATAACAAATAACAGCAGGGAAGAAAACGAGGAAATGTTGACTTTGAAATTGCAAGAGTTAGCAATAGAGATTCAAGAGATTAGTGCTGAATCCGCAGACTGTAAGGAAGCTAACACAGAGAATATAGAGGATTACCGTACCGAATTTACGAGACGCCAAGGGGATAAGCTCATAGCAAGGCTGGGAAATTTCTCTAACACCCTTAAACAACTATGTGATCTTCTCCAAGAGTGTAAATGA
Protein sequence

>MS.gene059085.t1

MTENPRDAVGNRRTRSQAAPEWTLTESLILLNEIAAVEAECSVNFSSYQQWDIISQNCAALDVDRNLAQCRRKWRSLLAEYEDVRRRRRAWNSDRELFDAVERVVKGREERGEVDRESDGELGNEERDVTVEIDMIGTGFKRKRPRSKVERNSVQKPKKYWPDEPSENLKEKQDPEVIHEEVPENNHLEEVFLKDFLEKKPKLKSPAKRRPKHIEMPLQNLPEVEKHHDNEKPKLPEPASVGNITNNSREENEEMLTLKLQELAIEIQEISAESADCKEANTENIEDYRTEFTRRQGDKLIARLGNFSNTLKQLCDLLQECK