Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061359.t1 | XP_024636870.1 | 94.5 | 329 | 17 | 1 | 1 | 329 | 416 | 743 | 1.70E-162 | 582 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061359.t1 | Q9S9N5 | 74.4 | 332 | 74 | 4 | 2 | 324 | 408 | 737 | 3.8e-134 | 479.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061359.t1 | A0A396HYG4 | 94.5 | 329 | 17 | 1 | 1 | 329 | 416 | 743 | 1.2e-162 | 582.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene061359 | 0.820567 | 6.41E-53 | -1.69E-46 |
| MS.gene049343 | MS.gene061359 | 0.844223 | 8.63E-59 | -1.69E-46 |
| MS.gene050026 | MS.gene061359 | 0.808605 | 2.85E-50 | -1.69E-46 |
| MS.gene050156 | MS.gene061359 | 0.834688 | 2.59E-56 | -1.69E-46 |
| MS.gene050799 | MS.gene061359 | 0.807109 | 5.93E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene061359 | 0.812015 | 5.24E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene061359 | 0.858463 | 8.10E-63 | -1.69E-46 |
| MS.gene052773 | MS.gene061359 | 0.809668 | 1.69E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene061359 | 0.807617 | 4.63E-50 | -1.69E-46 |
| MS.gene053145 | MS.gene061359 | 0.840621 | 7.79E-58 | -1.69E-46 |
| MS.gene053202 | MS.gene061359 | 0.871491 | 6.56E-67 | -1.69E-46 |
| MS.gene053226 | MS.gene061359 | 0.811536 | 6.67E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene061359 | 0.802611 | 5.17E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene061359 | 0.834301 | 3.24E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene061359 | 0.816611 | 5.06E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene061359 | 0.826748 | 2.29E-54 | -1.69E-46 |
| MS.gene056050 | MS.gene061359 | 0.807574 | 4.73E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene061359 | 0.816963 | 4.22E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene061359 | 0.814083 | 1.85E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene061359 | 0.824954 | 6.10E-54 | -1.69E-46 |
| MS.gene058625 | MS.gene061359 | 0.828449 | 8.93E-55 | -1.69E-46 |
| MS.gene058783 | MS.gene061359 | 0.800696 | 1.28E-48 | -1.69E-46 |
| MS.gene058979 | MS.gene061359 | 0.827739 | 1.32E-54 | -1.69E-46 |
| MS.gene059085 | MS.gene061359 | 0.805885 | 1.08E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene061359 | 0.813916 | 2.01E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene061359 | 0.810934 | 9.00E-51 | -1.69E-46 |
| MS.gene060352 | MS.gene061359 | 0.841882 | 3.63E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061359.t1 | MTR_4g130820 | 72.424 | 330 | 70 | 6 | 2 | 324 | 412 | 727 | 1.99e-159 | 463 |
| MS.gene061359.t1 | MTR_3g091080 | 64.242 | 330 | 102 | 4 | 2 | 324 | 390 | 710 | 5.07e-145 | 426 |
| MS.gene061359.t1 | MTR_1g028370 | 62.908 | 337 | 104 | 7 | 2 | 329 | 392 | 716 | 3.56e-143 | 421 |
| MS.gene061359.t1 | MTR_2g460590 | 61.047 | 344 | 108 | 3 | 1 | 329 | 359 | 691 | 1.18e-142 | 419 |
| MS.gene061359.t1 | MTR_2g094860 | 62.769 | 325 | 111 | 4 | 1 | 323 | 385 | 701 | 2.91e-140 | 413 |
| MS.gene061359.t1 | MTR_7g034605 | 62.270 | 326 | 112 | 4 | 2 | 323 | 360 | 678 | 5.16e-139 | 409 |
| MS.gene061359.t1 | MTR_3g109030 | 61.677 | 334 | 117 | 2 | 1 | 324 | 389 | 721 | 3.12e-137 | 406 |
| MS.gene061359.t1 | MTR_3g088875 | 57.849 | 344 | 125 | 6 | 2 | 328 | 352 | 692 | 5.73e-134 | 397 |
| MS.gene061359.t1 | MTR_1g064240 | 59.412 | 340 | 117 | 6 | 1 | 327 | 379 | 710 | 7.62e-134 | 397 |
| MS.gene061359.t1 | MTR_4g058730 | 72.112 | 251 | 70 | 0 | 1 | 251 | 367 | 617 | 1.80e-132 | 390 |
| MS.gene061359.t1 | MTR_7g117310 | 59.394 | 330 | 118 | 5 | 2 | 324 | 341 | 661 | 8.94e-132 | 390 |
| MS.gene061359.t1 | MTR_7g117310 | 59.394 | 330 | 118 | 5 | 2 | 324 | 395 | 715 | 4.18e-131 | 390 |
| MS.gene061359.t1 | MTR_7g117310 | 59.394 | 330 | 118 | 5 | 2 | 324 | 407 | 727 | 4.81e-131 | 390 |
| MS.gene061359.t1 | MTR_8g027755 | 57.622 | 328 | 125 | 4 | 1 | 322 | 398 | 717 | 2.31e-129 | 386 |
| MS.gene061359.t1 | MTR_2g081110 | 44.578 | 249 | 135 | 2 | 2 | 249 | 377 | 623 | 1.26e-68 | 226 |
| MS.gene061359.t1 | MTR_8g464570 | 43.775 | 249 | 137 | 2 | 2 | 249 | 381 | 627 | 1.87e-66 | 221 |
| MS.gene061359.t1 | MTR_7g012260 | 41.975 | 243 | 138 | 2 | 10 | 251 | 437 | 677 | 4.15e-63 | 213 |
| MS.gene061359.t1 | MTR_6g477760 | 44.141 | 256 | 123 | 6 | 8 | 251 | 445 | 692 | 1.18e-56 | 195 |
| MS.gene061359.t1 | MTR_6g075290 | 43.678 | 261 | 129 | 4 | 8 | 257 | 308 | 561 | 2.15e-53 | 184 |
| MS.gene061359.t1 | MTR_6g075440 | 39.721 | 287 | 145 | 7 | 1 | 267 | 477 | 755 | 4.27e-53 | 186 |
| MS.gene061359.t1 | MTR_6g075440 | 40.000 | 280 | 143 | 7 | 1 | 266 | 477 | 745 | 6.51e-53 | 186 |
| MS.gene061359.t1 | MTR_6g075290 | 43.678 | 261 | 129 | 4 | 8 | 257 | 469 | 722 | 1.15e-52 | 184 |
| MS.gene061359.t1 | MTR_6g075290 | 43.678 | 261 | 129 | 4 | 8 | 257 | 510 | 763 | 1.82e-52 | 184 |
| MS.gene061359.t1 | MTR_5g007630 | 40.377 | 265 | 135 | 6 | 12 | 263 | 483 | 737 | 1.90e-50 | 179 |
| MS.gene061359.t1 | MTR_6g076060 | 42.231 | 251 | 125 | 5 | 8 | 245 | 212 | 455 | 8.86e-50 | 173 |
| MS.gene061359.t1 | MTR_6g075390 | 38.075 | 239 | 121 | 6 | 8 | 226 | 427 | 658 | 8.34e-39 | 145 |
| MS.gene061359.t1 | MTR_6g075410 | 37.109 | 256 | 138 | 6 | 10 | 252 | 415 | 660 | 2.68e-38 | 144 |
| MS.gene061359.t1 | MTR_4g113530 | 25.904 | 166 | 120 | 2 | 3 | 167 | 278 | 441 | 8.83e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061359.t1 | AT1G19780 | 76.627 | 338 | 69 | 4 | 2 | 329 | 203 | 540 | 2.61e-180 | 509 |
| MS.gene061359.t1 | AT1G19780 | 76.627 | 338 | 69 | 4 | 2 | 329 | 414 | 751 | 8.60e-178 | 511 |
| MS.gene061359.t1 | AT1G15990 | 76.807 | 332 | 66 | 5 | 2 | 324 | 408 | 737 | 3.50e-173 | 498 |
| MS.gene061359.t1 | AT4G30560 | 73.716 | 331 | 59 | 4 | 2 | 324 | 393 | 703 | 4.85e-171 | 492 |
| MS.gene061359.t1 | AT4G30560 | 73.716 | 331 | 59 | 4 | 2 | 324 | 393 | 703 | 4.85e-171 | 492 |
| MS.gene061359.t1 | AT4G30560 | 73.716 | 331 | 59 | 4 | 2 | 324 | 393 | 703 | 4.85e-171 | 492 |
| MS.gene061359.t1 | AT4G30560 | 73.716 | 331 | 59 | 4 | 2 | 324 | 419 | 729 | 9.09e-171 | 492 |
| MS.gene061359.t1 | AT4G30560 | 73.716 | 331 | 59 | 4 | 2 | 324 | 419 | 729 | 9.09e-171 | 492 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 393 | 717 | 1.46e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 420 | 744 | 3.90e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 420 | 744 | 3.90e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 420 | 744 | 3.90e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 420 | 744 | 3.90e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 420 | 744 | 3.90e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 443 | 767 | 7.37e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 443 | 767 | 7.37e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 443 | 767 | 7.37e-166 | 481 |
| MS.gene061359.t1 | AT2G23980 | 71.386 | 332 | 79 | 5 | 2 | 324 | 443 | 767 | 7.37e-166 | 481 |
| MS.gene061359.t1 | AT5G57940 | 70.769 | 325 | 81 | 3 | 2 | 324 | 397 | 709 | 8.84e-162 | 468 |
| MS.gene061359.t1 | AT5G57940 | 70.769 | 325 | 81 | 3 | 2 | 324 | 404 | 716 | 1.16e-161 | 468 |
| MS.gene061359.t1 | AT5G57940 | 70.769 | 325 | 81 | 3 | 2 | 324 | 404 | 716 | 1.16e-161 | 468 |
| MS.gene061359.t1 | AT2G24610 | 61.224 | 343 | 110 | 5 | 1 | 324 | 386 | 724 | 5.22e-139 | 410 |
| MS.gene061359.t1 | AT2G24610 | 61.224 | 343 | 110 | 5 | 1 | 324 | 401 | 739 | 9.93e-139 | 410 |
| MS.gene061359.t1 | AT2G28260 | 62.037 | 324 | 101 | 1 | 1 | 324 | 336 | 637 | 1.48e-138 | 407 |
| MS.gene061359.t1 | AT4G01010 | 60.542 | 332 | 108 | 4 | 2 | 326 | 380 | 695 | 2.25e-138 | 408 |
| MS.gene061359.t1 | AT4G01010 | 60.542 | 332 | 108 | 4 | 2 | 326 | 380 | 695 | 2.25e-138 | 408 |
| MS.gene061359.t1 | AT5G53130 | 60.423 | 331 | 115 | 3 | 2 | 324 | 392 | 714 | 3.66e-138 | 408 |
| MS.gene061359.t1 | AT2G28260 | 62.037 | 324 | 101 | 1 | 1 | 324 | 376 | 677 | 5.10e-138 | 407 |
| MS.gene061359.t1 | AT4G30360 | 62.500 | 336 | 117 | 3 | 1 | 328 | 386 | 720 | 4.61e-137 | 405 |
| MS.gene061359.t1 | AT1G01340 | 60.121 | 331 | 120 | 4 | 2 | 324 | 374 | 700 | 1.19e-136 | 404 |
| MS.gene061359.t1 | AT1G01340 | 60.121 | 331 | 120 | 4 | 2 | 324 | 379 | 705 | 1.56e-136 | 404 |
| MS.gene061359.t1 | AT5G14870 | 56.484 | 347 | 127 | 4 | 1 | 323 | 354 | 700 | 6.73e-132 | 392 |
| MS.gene061359.t1 | AT2G46430 | 57.879 | 330 | 119 | 7 | 2 | 322 | 383 | 701 | 7.14e-126 | 376 |
| MS.gene061359.t1 | AT2G46430 | 57.879 | 330 | 119 | 7 | 2 | 322 | 383 | 701 | 7.14e-126 | 376 |
| MS.gene061359.t1 | AT3G48010 | 57.827 | 313 | 113 | 2 | 2 | 299 | 363 | 671 | 1.12e-124 | 373 |
| MS.gene061359.t1 | AT2G46440 | 53.425 | 292 | 123 | 4 | 2 | 291 | 163 | 443 | 6.19e-95 | 289 |
| MS.gene061359.t1 | AT2G46440 | 53.425 | 292 | 123 | 4 | 2 | 291 | 341 | 621 | 7.48e-93 | 289 |
| MS.gene061359.t1 | AT2G46450 | 43.077 | 325 | 153 | 8 | 2 | 320 | 305 | 603 | 8.06e-81 | 257 |
| MS.gene061359.t1 | AT2G46450 | 43.077 | 325 | 153 | 8 | 2 | 320 | 318 | 616 | 9.17e-81 | 258 |
| MS.gene061359.t1 | AT2G46450 | 43.077 | 325 | 153 | 8 | 2 | 320 | 341 | 639 | 1.66e-80 | 257 |
| MS.gene061359.t1 | AT2G46450 | 43.077 | 325 | 153 | 8 | 2 | 320 | 341 | 639 | 1.66e-80 | 257 |
| MS.gene061359.t1 | AT5G54250 | 43.373 | 249 | 138 | 2 | 2 | 249 | 402 | 648 | 3.44e-65 | 218 |
| MS.gene061359.t1 | AT5G54250 | 43.373 | 249 | 138 | 2 | 2 | 249 | 402 | 648 | 3.44e-65 | 218 |
| MS.gene061359.t1 | AT5G54250 | 43.373 | 249 | 138 | 2 | 2 | 249 | 402 | 648 | 3.44e-65 | 218 |
| MS.gene061359.t1 | AT5G15410 | 41.702 | 235 | 134 | 2 | 10 | 243 | 444 | 676 | 7.04e-60 | 204 |
| MS.gene061359.t1 | AT5G54250 | 41.767 | 249 | 137 | 3 | 2 | 249 | 402 | 643 | 7.42e-60 | 204 |
| MS.gene061359.t1 | AT5G15410 | 41.702 | 235 | 134 | 2 | 10 | 243 | 445 | 677 | 7.51e-60 | 204 |
| MS.gene061359.t1 | AT3G17690 | 43.678 | 261 | 131 | 6 | 1 | 252 | 466 | 719 | 1.36e-58 | 201 |
| MS.gene061359.t1 | AT3G17700 | 42.798 | 243 | 121 | 5 | 12 | 243 | 510 | 745 | 2.73e-52 | 184 |
| MS.gene061359.t1 | AT3G17690 | 48.000 | 175 | 84 | 2 | 1 | 175 | 466 | 633 | 4.26e-46 | 166 |
| MS.gene061359.t1 | AT3G17700 | 45.833 | 168 | 84 | 2 | 12 | 179 | 510 | 670 | 3.62e-41 | 152 |
| MS.gene061359.t1 | AT3G17690 | 56.863 | 51 | 22 | 0 | 1 | 51 | 466 | 516 | 7.19e-11 | 63.5 |
Find 80 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAAGCTTGCTGCAGATTT+TGG | 0.164117 | 8.2:+83095163 | None:intergenic |
| CCTCAAAGTTCGTCAAAATT+TGG | 0.172842 | 8.2:-83094860 | MS.gene061359:CDS |
| TTCTGCAAGGGCGTTGGTTC+CGG | 0.218060 | 8.2:-83094885 | MS.gene061359:CDS |
| CTGCATGGAGGAAGCATTTA+AGG | 0.231374 | 8.2:-83094967 | MS.gene061359:CDS |
| ATATCTCTTCTAAGATCTTT+TGG | 0.250851 | 8.2:+83095582 | None:intergenic |
| CAGAGAAGACTTACATAGTT+AGG | 0.283571 | 8.2:-83095336 | MS.gene061359:CDS |
| AGGGGTCGATGAAGAAAATT+TGG | 0.285665 | 8.2:-83095616 | MS.gene061359:CDS |
| TCTGCAAGGGCGTTGGTTCC+GGG | 0.297464 | 8.2:-83094884 | MS.gene061359:CDS |
| GTGCTCTTTGCTCTTCTGAT+TGG | 0.318517 | 8.2:-83095886 | MS.gene061359:CDS |
| AATCAGGTTCTGAGGGCTTT+GGG | 0.347993 | 8.2:+83094747 | None:intergenic |
| AAATCAGGTTCTGAGGGCTT+TGG | 0.350213 | 8.2:+83094746 | None:intergenic |
| TCAATCAATGTCGGTTCGTC+TGG | 0.370374 | 8.2:-83095754 | MS.gene061359:CDS |
| ATACAACAGTCTATGCTTCT+CGG | 0.384722 | 8.2:-83094832 | MS.gene061359:CDS |
| TCATTGAGAGCACTGAAATC+AGG | 0.396573 | 8.2:+83094731 | None:intergenic |
| CTTGCAGAATCATCACTTCC+CGG | 0.414286 | 8.2:+83094899 | None:intergenic |
| CGGTTTGCTGCTAATCTTCG+TGG | 0.417955 | 8.2:-83094812 | MS.gene061359:CDS |
| ATGAACAGCATCTCATCAAC+TGG | 0.419571 | 8.2:+83095304 | None:intergenic |
| TTCACGGTTCTTGTTGATGA+TGG | 0.422131 | 8.2:+83095142 | None:intergenic |
| TACCTCACTCATAGCTTTCA+CGG | 0.425446 | 8.2:+83095126 | None:intergenic |
| TCCATTGTTGCGAATAGAAA+CGG | 0.447494 | 8.2:+83095017 | None:intergenic |
| GGGTGTTCAACCACTTGTAT+TGG | 0.454525 | 8.2:+83095640 | None:intergenic |
| GCATCATCGCTTGCTTCCTC+CGG | 0.465125 | 8.2:-83095691 | MS.gene061359:CDS |
| GCTTGCTTCCTCCGGACCTA+AGG | 0.465489 | 8.2:-83095683 | MS.gene061359:CDS |
| TTCACCAGCTCTCTTGAACT+TGG | 0.484399 | 8.2:+83094773 | None:intergenic |
| CTGACTCTTTCCCTTAGGTC+CGG | 0.486118 | 8.2:+83095672 | None:intergenic |
| AGCGTCTGACTCTTTCCCTT+AGG | 0.488363 | 8.2:+83095667 | None:intergenic |
| AGAGAAGACTTACATAGTTA+GGG | 0.493349 | 8.2:-83095335 | MS.gene061359:CDS |
| GGTTCCTCTCTTTGCTAACA+TGG | 0.504157 | 8.2:-83095410 | MS.gene061359:CDS |
| GGCATTTGCTTTAGAAGCAG+AGG | 0.505619 | 8.2:-83095101 | MS.gene061359:CDS |
| TTTGTTTGAATTTAGTCAGA+AGG | 0.518058 | 8.2:-83095548 | MS.gene061359:CDS |
| TAGACACATACATGGTAGAC+AGG | 0.521475 | 8.2:-83095053 | MS.gene061359:CDS |
| ATTCGCAACAATGGAGAACA+TGG | 0.522904 | 8.2:-83095009 | MS.gene061359:CDS |
| GACGAACCGACATTGATTGA+AGG | 0.524267 | 8.2:+83095757 | None:intergenic |
| TGGAGGAAATGAGGATCAAA+AGG | 0.526808 | 8.2:-83095734 | MS.gene061359:CDS |
| TCCGTTTCTATTCGCAACAA+TGG | 0.528384 | 8.2:-83095018 | MS.gene061359:CDS |
| AGAGTTGCTAACATGGGCAT+TGG | 0.533440 | 8.2:-83095188 | MS.gene061359:CDS |
| TGATGATTCTGCAAGGGCGT+TGG | 0.534637 | 8.2:-83094891 | MS.gene061359:CDS |
| GAAAGCTATGAGTGAGGTAG+AGG | 0.537681 | 8.2:-83095122 | MS.gene061359:CDS |
| CTAGAGAGTGTTACTACAGA+TGG | 0.541421 | 8.2:-83095268 | MS.gene061359:CDS |
| GTCTTCTCTGTATATAAACT+CGG | 0.543647 | 8.2:+83095349 | None:intergenic |
| ATCAATGTCGGTTCGTCTGG+AGG | 0.548219 | 8.2:-83095751 | MS.gene061359:CDS |
| TTGTTTGAATTTAGTCAGAA+GGG | 0.551796 | 8.2:-83095547 | MS.gene061359:intron |
| GCACTGAAATCAGGTTCTGA+GGG | 0.551873 | 8.2:+83094740 | None:intergenic |
| AGAAGTGGCTTCTTCAACAG+AGG | 0.551875 | 8.2:-83095241 | MS.gene061359:CDS |
| CTTGCTTCCTCCGGACCTAA+GGG | 0.552988 | 8.2:-83095682 | MS.gene061359:CDS |
| AGCACTGAAATCAGGTTCTG+AGG | 0.553944 | 8.2:+83094739 | None:intergenic |
| TTTGACGAACTTTGAGGACC+CGG | 0.557037 | 8.2:+83094866 | None:intergenic |
| TTGCAGAATCATCACTTCCC+GGG | 0.558557 | 8.2:+83094900 | None:intergenic |
| CGGTTCGTCTGGAGGAAATG+AGG | 0.562037 | 8.2:-83095743 | MS.gene061359:CDS |
| AGTACCAAGTTCAAGAGAGC+TGG | 0.565279 | 8.2:-83094777 | MS.gene061359:CDS |
| TCTTCTGATTGGAAACATGC+AGG | 0.565687 | 8.2:-83095875 | MS.gene061359:intron |
| GAGATGCTGTTCATCATTCG+TGG | 0.566373 | 8.2:-83095295 | MS.gene061359:CDS |
| ACTACAGATGGTGGAAGAAG+TGG | 0.567107 | 8.2:-83095256 | MS.gene061359:CDS |
| ATACTACGAATCTGATTACC+CGG | 0.576933 | 8.2:-83094918 | MS.gene061359:CDS |
| TTTAAGGCGAAGGCGAAGGA+AGG | 0.579780 | 8.2:-83094951 | MS.gene061359:CDS |
| AGCATTTAAGGCGAAGGCGA+AGG | 0.589667 | 8.2:-83094955 | MS.gene061359:CDS |
| CAATCTATATTCAAGCTGCA+TGG | 0.590954 | 8.2:-83094982 | MS.gene061359:CDS |
| GGAGGAAGCATTTAAGGCGA+AGG | 0.591557 | 8.2:-83094961 | MS.gene061359:CDS |
| GCGATGATGCATCCACTGCT+CGG | 0.598686 | 8.2:+83095704 | None:intergenic |
| GAGAGTGTTACTACAGATGG+TGG | 0.613056 | 8.2:-83095265 | MS.gene061359:CDS |
| GACCTACCTTCAATCAATGT+CGG | 0.613772 | 8.2:-83095763 | MS.gene061359:CDS |
| AAGGCGAAGGCGAAGGAAGG+AGG | 0.615424 | 8.2:-83094948 | MS.gene061359:CDS |
| ACTCTTTCCCTTAGGTCCGG+AGG | 0.617015 | 8.2:+83095675 | None:intergenic |
| AAAGGCGTGACTCCGAGCAG+TGG | 0.621729 | 8.2:-83095716 | MS.gene061359:CDS |
| GTGGTGAAGAGTTGCTAACA+TGG | 0.626286 | 8.2:-83095195 | MS.gene061359:CDS |
| CGGGAAGTGATGATTCTGCA+AGG | 0.626896 | 8.2:-83094898 | MS.gene061359:CDS |
| AAGACTTACATAGTTAGGGA+AGG | 0.629987 | 8.2:-83095331 | MS.gene061359:CDS |
| TACAAGTGGTTGAACACCCG+AGG | 0.630986 | 8.2:-83095636 | MS.gene061359:CDS |
| GACGCTACGACCAATACAAG+TGG | 0.637852 | 8.2:-83095650 | MS.gene061359:CDS |
| TCGTCCATGTTAGCAAAGAG+AGG | 0.645418 | 8.2:+83095406 | None:intergenic |
| GGGAAGTGATGATTCTGCAA+GGG | 0.645571 | 8.2:-83094897 | MS.gene061359:CDS |
| TCACAGTTTAGACACATACA+TGG | 0.659888 | 8.2:-83095061 | MS.gene061359:CDS |
| ACAAGTGGTTGAACACCCGA+GGG | 0.676415 | 8.2:-83095635 | MS.gene061359:CDS |
| AACCGTGAAAGCTATGAGTG+AGG | 0.683734 | 8.2:-83095128 | MS.gene061359:CDS |
| TTCGCAACAATGGAGAACAT+GGG | 0.684012 | 8.2:-83095008 | MS.gene061359:CDS |
| TACTACGAATCTGATTACCC+GGG | 0.696386 | 8.2:-83094917 | MS.gene061359:CDS |
| TGGTGAAGAGTTGCTAACAT+GGG | 0.697702 | 8.2:-83095194 | MS.gene061359:CDS |
| AACCGACATTGATTGAAGGT+AGG | 0.702813 | 8.2:+83095761 | None:intergenic |
| TCTATATTCAAGCTGCATGG+AGG | 0.707083 | 8.2:-83094979 | MS.gene061359:CDS |
| CAAGTGGTTGAACACCCGAG+GGG | 0.768003 | 8.2:-83095634 | MS.gene061359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTTACAGTAAAAACTTT+CGG | + | chr8.2:83094771-83094790 | None:intergenic | 20.0% |
| ! | GTGTATGACATATATTATTC+AGG | - | chr8.2:83095185-83095204 | MS.gene061359:CDS | 25.0% |
| ! | TGAGAAAATAAAGAGTTAGA+AGG | + | chr8.2:83095155-83095174 | None:intergenic | 25.0% |
| ! | TTGTTTGAATTTAGTCAGAA+GGG | - | chr8.2:83095069-83095088 | MS.gene061359:CDS | 25.0% |
| ! | TTTGTTTGAATTTAGTCAGA+AGG | - | chr8.2:83095068-83095087 | MS.gene061359:CDS | 25.0% |
| !!! | ATATCTCTTCTAAGATCTTT+TGG | + | chr8.2:83095037-83095056 | None:intergenic | 25.0% |
| AGAGAAGACTTACATAGTTA+GGG | - | chr8.2:83095281-83095300 | MS.gene061359:CDS | 30.0% | |
| CCAATTGCATTTATAACACA+AGG | + | chr8.2:83094830-83094849 | None:intergenic | 30.0% | |
| CCTTGTGTTATAAATGCAAT+TGG | - | chr8.2:83094827-83094846 | MS.gene061359:CDS | 30.0% | |
| GTCTTCTCTGTATATAAACT+CGG | + | chr8.2:83095270-83095289 | None:intergenic | 30.0% | |
| TGCAATGAGAAATTTCAGAA+AGG | + | chr8.2:83095119-83095138 | None:intergenic | 30.0% | |
| AAGACTTACATAGTTAGGGA+AGG | - | chr8.2:83095285-83095304 | MS.gene061359:CDS | 35.0% | |
| ATACAACAGTCTATGCTTCT+CGG | - | chr8.2:83095784-83095803 | MS.gene061359:intron | 35.0% | |
| ATACTACGAATCTGATTACC+CGG | - | chr8.2:83095698-83095717 | MS.gene061359:CDS | 35.0% | |
| CAATCTATATTCAAGCTGCA+TGG | - | chr8.2:83095634-83095653 | MS.gene061359:CDS | 35.0% | |
| CAGAGAAGACTTACATAGTT+AGG | - | chr8.2:83095280-83095299 | MS.gene061359:CDS | 35.0% | |
| CCTCAAAGTTCGTCAAAATT+TGG | - | chr8.2:83095756-83095775 | MS.gene061359:CDS | 35.0% | |
| TCACAGTTTAGACACATACA+TGG | - | chr8.2:83095555-83095574 | MS.gene061359:CDS | 35.0% | |
| TCCATTGTTGCGAATAGAAA+CGG | + | chr8.2:83095602-83095621 | None:intergenic | 35.0% | |
| ! | TTGAAGGAAGGTGATTTTTG+TGG | - | chr8.2:83095402-83095421 | MS.gene061359:CDS | 35.0% |
| !! | CCAAATTTTGACGAACTTTG+AGG | + | chr8.2:83095759-83095778 | None:intergenic | 35.0% |
| AGGGGTCGATGAAGAAAATT+TGG | - | chr8.2:83095000-83095019 | MS.gene061359:CDS | 40.0% | |
| ATGAACAGCATCTCATCAAC+TGG | + | chr8.2:83095315-83095334 | None:intergenic | 40.0% | |
| ATTCGCAACAATGGAGAACA+TGG | - | chr8.2:83095607-83095626 | MS.gene061359:CDS | 40.0% | |
| GACCTACCTTCAATCAATGT+CGG | - | chr8.2:83094853-83094872 | MS.gene061359:CDS | 40.0% | |
| TACCTCACTCATAGCTTTCA+CGG | + | chr8.2:83095493-83095512 | None:intergenic | 40.0% | |
| TACTACGAATCTGATTACCC+GGG | - | chr8.2:83095699-83095718 | MS.gene061359:CDS | 40.0% | |
| TAGACACATACATGGTAGAC+AGG | - | chr8.2:83095563-83095582 | MS.gene061359:CDS | 40.0% | |
| TCCGTTTCTATTCGCAACAA+TGG | - | chr8.2:83095598-83095617 | MS.gene061359:CDS | 40.0% | |
| TCTATATTCAAGCTGCATGG+AGG | - | chr8.2:83095637-83095656 | MS.gene061359:CDS | 40.0% | |
| TCTTCTGATTGGAAACATGC+AGG | - | chr8.2:83094741-83094760 | MS.gene061359:CDS | 40.0% | |
| TGGAGGAAATGAGGATCAAA+AGG | - | chr8.2:83094882-83094901 | MS.gene061359:CDS | 40.0% | |
| TGGTGAAGAGTTGCTAACAT+GGG | - | chr8.2:83095422-83095441 | MS.gene061359:intron | 40.0% | |
| TTCGCAACAATGGAGAACAT+GGG | - | chr8.2:83095608-83095627 | MS.gene061359:CDS | 40.0% | |
| ! | AACCGACATTGATTGAAGGT+AGG | + | chr8.2:83094858-83094877 | None:intergenic | 40.0% |
| ! | AATTTTCTTCATCGACCCCT+CGG | + | chr8.2:83095000-83095019 | None:intergenic | 40.0% |
| ! | TTCACGGTTCTTGTTGATGA+TGG | + | chr8.2:83095477-83095496 | None:intergenic | 40.0% |
| !! | CTAGAGAGTGTTACTACAGA+TGG | - | chr8.2:83095348-83095367 | MS.gene061359:CDS | 40.0% |
| !! | CTTCAACAGAGGAGTTTTGA+AGG | - | chr8.2:83095386-83095405 | MS.gene061359:CDS | 40.0% |
| !! | TCATTGAGAGCACTGAAATC+AGG | + | chr8.2:83095888-83095907 | None:intergenic | 40.0% |
| !!! | AACAGAGGAGTTTTGAAGGA+AGG | - | chr8.2:83095390-83095409 | MS.gene061359:CDS | 40.0% |
| AACCGTGAAAGCTATGAGTG+AGG | - | chr8.2:83095488-83095507 | MS.gene061359:intron | 45.0% | |
| AGAAGTGGCTTCTTCAACAG+AGG | - | chr8.2:83095375-83095394 | MS.gene061359:CDS | 45.0% | |
| AGAGTTGCTAACATGGGCAT+TGG | - | chr8.2:83095428-83095447 | MS.gene061359:intron | 45.0% | |
| AGTACCAAGTTCAAGAGAGC+TGG | - | chr8.2:83095839-83095858 | MS.gene061359:intron | 45.0% | |
| CTTGCAGAATCATCACTTCC+CGG | + | chr8.2:83095720-83095739 | None:intergenic | 45.0% | |
| GAAAGCTATGAGTGAGGTAG+AGG | - | chr8.2:83095494-83095513 | MS.gene061359:intron | 45.0% | |
| GAGATGCTGTTCATCATTCG+TGG | - | chr8.2:83095321-83095340 | MS.gene061359:CDS | 45.0% | |
| GGGAAGTGATGATTCTGCAA+GGG | - | chr8.2:83095719-83095738 | MS.gene061359:CDS | 45.0% | |
| GGGTGTTCAACCACTTGTAT+TGG | + | chr8.2:83094979-83094998 | None:intergenic | 45.0% | |
| GGTTCCTCTCTTTGCTAACA+TGG | - | chr8.2:83095206-83095225 | MS.gene061359:CDS | 45.0% | |
| GTGCTCTTTGCTCTTCTGAT+TGG | - | chr8.2:83094730-83094749 | MS.gene061359:CDS | 45.0% | |
| GTGGTGAAGAGTTGCTAACA+TGG | - | chr8.2:83095421-83095440 | MS.gene061359:intron | 45.0% | |
| TCGTCCATGTTAGCAAAGAG+AGG | + | chr8.2:83095213-83095232 | None:intergenic | 45.0% | |
| TTCACCAGCTCTCTTGAACT+TGG | + | chr8.2:83095846-83095865 | None:intergenic | 45.0% | |
| TTGCAGAATCATCACTTCCC+GGG | + | chr8.2:83095719-83095738 | None:intergenic | 45.0% | |
| ! | AGCACTGAAATCAGGTTCTG+AGG | + | chr8.2:83095880-83095899 | None:intergenic | 45.0% |
| ! | ATTTTCTTCATCGACCCCTC+GGG | + | chr8.2:83094999-83095018 | None:intergenic | 45.0% |
| ! | GACGAACCGACATTGATTGA+AGG | + | chr8.2:83094862-83094881 | None:intergenic | 45.0% |
| ! | GCACTGAAATCAGGTTCTGA+GGG | + | chr8.2:83095879-83095898 | None:intergenic | 45.0% |
| ! | TTTGACGAACTTTGAGGACC+CGG | + | chr8.2:83095753-83095772 | None:intergenic | 45.0% |
| !! | AAATCAGGTTCTGAGGGCTT+TGG | + | chr8.2:83095873-83095892 | None:intergenic | 45.0% |
| !! | AATCAGGTTCTGAGGGCTTT+GGG | + | chr8.2:83095872-83095891 | None:intergenic | 45.0% |
| !! | ACTACAGATGGTGGAAGAAG+TGG | - | chr8.2:83095360-83095379 | MS.gene061359:CDS | 45.0% |
| !! | CTGCATGGAGGAAGCATTTA+AGG | - | chr8.2:83095649-83095668 | MS.gene061359:CDS | 45.0% |
| !! | GAGAGTGTTACTACAGATGG+TGG | - | chr8.2:83095351-83095370 | MS.gene061359:CDS | 45.0% |
| !! | GGCATTTGCTTTAGAAGCAG+AGG | - | chr8.2:83095515-83095534 | MS.gene061359:intron | 45.0% |
| !! | TCAATCAATGTCGGTTCGTC+TGG | - | chr8.2:83094862-83094881 | MS.gene061359:CDS | 45.0% |
| ACAAGTGGTTGAACACCCGA+GGG | - | chr8.2:83094981-83095000 | MS.gene061359:CDS | 50.0% | |
| AGCGTCTGACTCTTTCCCTT+AGG | + | chr8.2:83094952-83094971 | None:intergenic | 50.0% | |
| CGGGAAGTGATGATTCTGCA+AGG | - | chr8.2:83095718-83095737 | MS.gene061359:CDS | 50.0% | |
| CTGACTCTTTCCCTTAGGTC+CGG | + | chr8.2:83094947-83094966 | None:intergenic | 50.0% | |
| GACGCTACGACCAATACAAG+TGG | - | chr8.2:83094966-83094985 | MS.gene061359:CDS | 50.0% | |
| TACAAGTGGTTGAACACCCG+AGG | - | chr8.2:83094980-83094999 | MS.gene061359:CDS | 50.0% | |
| ! | AGCATTTAAGGCGAAGGCGA+AGG | - | chr8.2:83095661-83095680 | MS.gene061359:CDS | 50.0% |
| ! | TGATGATTCTGCAAGGGCGT+TGG | - | chr8.2:83095725-83095744 | MS.gene061359:CDS | 50.0% |
| ! | TTTAAGGCGAAGGCGAAGGA+AGG | - | chr8.2:83095665-83095684 | MS.gene061359:CDS | 50.0% |
| !! | ATCAATGTCGGTTCGTCTGG+AGG | - | chr8.2:83094865-83094884 | MS.gene061359:CDS | 50.0% |
| !! | CGGTTTGCTGCTAATCTTCG+TGG | - | chr8.2:83095804-83095823 | MS.gene061359:intron | 50.0% |
| !! | GGAGGAAGCATTTAAGGCGA+AGG | - | chr8.2:83095655-83095674 | MS.gene061359:CDS | 50.0% |
| !!! | GGCAAGCTTGCTGCAGATTT+TGG | + | chr8.2:83095456-83095475 | None:intergenic | 50.0% |
| ACTCTTTCCCTTAGGTCCGG+AGG | + | chr8.2:83094944-83094963 | None:intergenic | 55.0% | |
| CAAGTGGTTGAACACCCGAG+GGG | - | chr8.2:83094982-83095001 | MS.gene061359:CDS | 55.0% | |
| CGGTTCGTCTGGAGGAAATG+AGG | - | chr8.2:83094873-83094892 | MS.gene061359:CDS | 55.0% | |
| CTTGCTTCCTCCGGACCTAA+GGG | - | chr8.2:83094934-83094953 | MS.gene061359:CDS | 55.0% | |
| GCATCATCGCTTGCTTCCTC+CGG | - | chr8.2:83094925-83094944 | MS.gene061359:CDS | 55.0% | |
| GCGATGATGCATCCACTGCT+CGG | + | chr8.2:83094915-83094934 | None:intergenic | 55.0% | |
| !! | TTCTGCAAGGGCGTTGGTTC+CGG | - | chr8.2:83095731-83095750 | MS.gene061359:CDS | 55.0% |
| AAGGCGAAGGCGAAGGAAGG+AGG | - | chr8.2:83095668-83095687 | MS.gene061359:CDS | 60.0% | |
| GCTTGCTTCCTCCGGACCTA+AGG | - | chr8.2:83094933-83094952 | MS.gene061359:CDS | 60.0% | |
| ! | AAAGGCGTGACTCCGAGCAG+TGG | - | chr8.2:83094900-83094919 | MS.gene061359:CDS | 60.0% |
| !! | TCTGCAAGGGCGTTGGTTCC+GGG | - | chr8.2:83095732-83095751 | MS.gene061359:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 83094721 | 83095917 | 83094721 | ID=MS.gene061359 |
| chr8.2 | mRNA | 83094721 | 83095917 | 83094721 | ID=MS.gene061359.t1;Parent=MS.gene061359 |
| chr8.2 | exon | 83095876 | 83095917 | 83095876 | ID=MS.gene061359.t1.exon1;Parent=MS.gene061359.t1 |
| chr8.2 | CDS | 83095876 | 83095917 | 83095876 | ID=cds.MS.gene061359.t1;Parent=MS.gene061359.t1 |
| chr8.2 | exon | 83095548 | 83095784 | 83095548 | ID=MS.gene061359.t1.exon2;Parent=MS.gene061359.t1 |
| chr8.2 | CDS | 83095548 | 83095784 | 83095548 | ID=cds.MS.gene061359.t1;Parent=MS.gene061359.t1 |
| chr8.2 | exon | 83094721 | 83095431 | 83094721 | ID=MS.gene061359.t1.exon3;Parent=MS.gene061359.t1 |
| chr8.2 | CDS | 83094721 | 83095431 | 83094721 | ID=cds.MS.gene061359.t1;Parent=MS.gene061359.t1 |
| Gene Sequence |
| Protein sequence |
