Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059330.t1 | XP_003591980.1 | 95 | 120 | 6 | 0 | 1 | 120 | 52 | 171 | 3.10E-61 | 244.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059330.t1 | G7I9N4 | 95.0 | 120 | 6 | 0 | 1 | 120 | 52 | 171 | 2.3e-61 | 244.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059330 | MS.gene33997 | 0.84013 | 1.05E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTGGTCTTTTTTA+GGG | - | 10759:9992-10011 | MS.gene059330:CDS | 15.0% |
| !!! | TTATTTTTTTGGTCTTTTTT+AGG | - | 10759:9991-10010 | MS.gene059330:CDS | 15.0% |
| !!! | TTGTGCTTATCTTATTTTTT+TGG | - | 10759:9980-9999 | MS.gene059330:CDS | 20.0% |
| ! | AATGAAATCAAAGATGATTC+AGG | - | 10759:9692-9711 | MS.gene059330:intron | 25.0% |
| ! | ATGCTCTTTACAATAATACA+TGG | - | 10759:9954-9973 | MS.gene059330:CDS | 25.0% |
| ! | ATGTTGTTGTGATTTATGTA+TGG | - | 10759:10023-10042 | MS.gene059330:CDS | 25.0% |
| ! | ATTGTATCAGACTTAAATAG+AGG | + | 10759:9842-9861 | MS.gene059330:intergenic | 25.0% |
| ! | GATTTCATTTGAATCAACAA+TGG | + | 10759:9681-9700 | MS.gene059330:intergenic | 25.0% |
| ! | TCTATTTAAGTCTGATACAA+TGG | - | 10759:9841-9860 | MS.gene059330:CDS | 25.0% |
| ! | TGCTCTTTACAATAATACAT+GGG | - | 10759:9955-9974 | MS.gene059330:CDS | 25.0% |
| !!! | TATGGTTTTTTATTGTGTTC+AGG | - | 10759:10041-10060 | MS.gene059330:CDS | 25.0% |
| ATGTAACACTTTAGTGAAAG+GGG | + | 10759:9915-9934 | MS.gene059330:intergenic | 30.0% | |
| CATGTAACACTTTAGTGAAA+GGG | + | 10759:9916-9935 | MS.gene059330:intergenic | 30.0% | |
| GTGATATTAGAAAACTCAAG+TGG | + | 10759:9584-9603 | MS.gene059330:intergenic | 30.0% | |
| TCATTTGAATCAACAATGGA+TGG | + | 10759:9677-9696 | MS.gene059330:intergenic | 30.0% | |
| AAATTTCTCAAGCTTAACCG+TGG | - | 10759:9800-9819 | MS.gene059330:CDS | 35.0% | |
| AACCTGAAACCTTGAAAACT+TGG | + | 10759:9778-9797 | MS.gene059330:intergenic | 35.0% | |
| AATTTCTCAAGCTTAACCGT+GGG | - | 10759:9801-9820 | MS.gene059330:CDS | 35.0% | |
| ACGGTTAAGCTTGAGAAATT+TGG | + | 10759:9801-9820 | MS.gene059330:intergenic | 35.0% | |
| ATACCAAACATTACCACCAA+TGG | + | 10759:9639-9658 | MS.gene059330:intergenic | 35.0% | |
| GCATGTAACACTTTAGTGAA+AGG | + | 10759:9917-9936 | MS.gene059330:intergenic | 35.0% | |
| TAATGTTTGGTATCTTGGAG+CGG | - | 10759:9646-9665 | MS.gene059330:intron | 35.0% | |
| ! | TTTTTATTGTGTTCAGGTGG+TGG | - | 10759:10047-10066 | MS.gene059330:CDS | 35.0% |
| !! | AGAGCATTGCAAATGAATTC+AGG | + | 10759:9941-9960 | MS.gene059330:intergenic | 35.0% |
| !!! | GGTTTTTTATTGTGTTCAGG+TGG | - | 10759:10044-10063 | MS.gene059330:CDS | 35.0% |
| !!! | TGGTCTTTTTTAGGGTTTAG+TGG | - | 10759:10000-10019 | MS.gene059330:CDS | 35.0% |
| AACACTTTAGTGAAAGGGGA+GGG | + | 10759:9911-9930 | MS.gene059330:intergenic | 40.0% | |
| CAAAGATGATTCAGGCAAGA+CGG | - | 10759:9700-9719 | MS.gene059330:intron | 40.0% | |
| GAAAACTTGGACATCCTCAA+TGG | + | 10759:9765-9784 | MS.gene059330:intergenic | 40.0% | |
| TAACACTTTAGTGAAAGGGG+AGG | + | 10759:9912-9931 | MS.gene059330:intergenic | 40.0% | |
| TTATCTACCTCCTACCATTG+AGG | - | 10759:9748-9767 | MS.gene059330:CDS | 40.0% | |
| ! | GGTGGTAATGTTTGGTATCT+TGG | - | 10759:9641-9660 | MS.gene059330:intron | 40.0% |
| ! | TGAGGATGTCCAAGTTTTCA+AGG | - | 10759:9766-9785 | MS.gene059330:CDS | 40.0% |
| !! | GTCCAAGTTTTCAAGGTTTC+AGG | - | 10759:9773-9792 | MS.gene059330:CDS | 40.0% |
| ACTTGGACATCCTCAATGGT+AGG | + | 10759:9761-9780 | MS.gene059330:intergenic | 45.0% | |
| ! | GGTCCATTGGTGGTAATGTT+TGG | - | 10759:9633-9652 | MS.gene059330:intron | 45.0% |
| CAAGTGTGACTCAGTGCCTT+GGG | - | 10759:9612-9631 | MS.gene059330:intron | 50.0% | |
| CATTACCACCAATGGACCCA+AGG | + | 10759:9631-9650 | MS.gene059330:intergenic | 50.0% | |
| TCAAGCTTAACCGTGGGACA+TGG | - | 10759:9807-9826 | MS.gene059330:CDS | 50.0% | |
| TGGACATCCTCAATGGTAGG+AGG | + | 10759:9758-9777 | MS.gene059330:intergenic | 50.0% | |
| ACTCAGTGCCTTGGGTCCAT+TGG | - | 10759:9620-9639 | MS.gene059330:intron | 55.0% | |
| GCAAGTGTGACTCAGTGCCT+TGG | - | 10759:9611-9630 | MS.gene059330:intron | 55.0% | |
| AACCGTGGGACATGGCATGC+TGG | - | 10759:9815-9834 | MS.gene059330:CDS | 60.0% | |
| ACGGTCGTGAAGTCGCACAG+TGG | - | 10759:9719-9738 | MS.gene059330:intron | 60.0% | |
| CAGTGCCTTGGGTCCATTGG+TGG | - | 10759:9623-9642 | MS.gene059330:intron | 60.0% | |
| GACCAGCATGCCATGTCCCA+CGG | + | 10759:9820-9839 | MS.gene059330:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10759 | gene | 9553 | 10089 | 9553 | ID=MS.gene059330 |
| 10759 | mRNA | 9553 | 10089 | 9553 | ID=MS.gene059330.t1;Parent=MS.gene059330 |
| 10759 | exon | 9747 | 10089 | 9747 | ID=MS.gene059330.t1.exon1;Parent=MS.gene059330.t1 |
| 10759 | CDS | 9747 | 10089 | 9747 | ID=cds.MS.gene059330.t1;Parent=MS.gene059330.t1 |
| 10759 | exon | 9553 | 9579 | 9553 | ID=MS.gene059330.t1.exon2;Parent=MS.gene059330.t1 |
| 10759 | CDS | 9553 | 9579 | 9553 | ID=cds.MS.gene059330.t1;Parent=MS.gene059330.t1 |
| Gene Sequence |
| Protein sequence |
