AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059415.t1 MTR_6g047750 96.802 469 15 0 1 469 194 662 0.0 934
MS.gene059415.t1 MTR_7g057230 53.004 466 186 9 16 468 211 656 1.17e-153 452
MS.gene059415.t1 MTR_3g089055 36.326 479 247 14 20 469 116 565 2.27e-82 265
MS.gene059415.t1 MTR_3g089055 36.326 479 247 14 20 469 121 570 2.54e-82 265
MS.gene059415.t1 MTR_1g029420 39.642 391 194 10 100 469 223 592 5.50e-78 254
MS.gene059415.t1 MTR_2g082090 34.362 486 261 15 17 469 119 579 2.16e-66 223
MS.gene059415.t1 MTR_5g097480 34.848 396 206 13 100 469 175 544 2.42e-63 214
MS.gene059415.t1 MTR_3g056110 35.567 388 214 11 100 469 173 542 4.51e-62 211
MS.gene059415.t1 MTR_2g026250 31.407 398 218 10 100 469 226 596 5.51e-58 201
MS.gene059415.t1 MTR_4g133660 32.474 388 225 9 100 468 173 542 2.22e-57 199
MS.gene059415.t1 MTR_5g094450 34.271 391 219 11 100 469 159 532 6.84e-57 197
MS.gene059415.t1 MTR_4g074320 53.368 193 76 4 42 229 2 185 1.60e-50 170
MS.gene059415.t1 MTR_4g074310 41.228 228 92 5 104 328 1 189 4.83e-44 154
MS.gene059415.t1 MTR_3g065980 30.435 391 229 12 101 469 171 540 3.91e-38 146
MS.gene059415.t1 MTR_8g442410 31.026 390 230 14 101 469 154 525 5.72e-36 140
MS.gene059415.t1 MTR_7g027190 29.383 405 216 13 103 469 301 673 4.52e-35 139
MS.gene059415.t1 MTR_2g097410 25.916 382 254 12 103 468 372 740 3.38e-31 127
MS.gene059415.t1 MTR_4g064150 24.740 384 264 8 100 468 359 732 4.60e-31 127
MS.gene059415.t1 MTR_4g104020 29.847 392 233 15 101 469 148 520 2.54e-30 123
MS.gene059415.t1 MTR_4g064200 26.121 379 253 10 103 468 285 649 5.82e-30 123
MS.gene059415.t1 MTR_2g097467 25.546 458 303 14 31 468 214 653 9.64e-29 120
MS.gene059415.t1 MTR_4g064160 25.916 382 258 9 100 468 314 683 3.59e-28 118
MS.gene059415.t1 MTR_2g097350 26.432 454 299 13 31 468 205 639 5.73e-28 117
MS.gene059415.t1 MTR_2g097463 27.798 277 182 6 201 468 387 654 1.17e-27 116
MS.gene059415.t1 MTR_4g097080 26.396 394 247 12 99 469 130 503 1.81e-27 115
MS.gene059415.t1 MTR_2g097473 26.453 344 226 9 138 468 324 653 1.62e-26 113
MS.gene059415.t1 MTR_7g062120 22.328 421 292 12 70 469 334 740 2.69e-26 112
MS.gene059415.t1 MTR_2g097390 26.239 343 229 10 138 468 310 640 9.06e-26 110
MS.gene059415.t1 MTR_4g102790 31.111 270 165 10 208 468 404 661 1.02e-24 107
MS.gene059415.t1 MTR_2g097310 24.719 445 305 13 37 468 210 637 1.23e-24 107
MS.gene059415.t1 MTR_5g015950 25.472 424 254 10 98 468 31 445 3.05e-23 102
MS.gene059415.t1 MTR_3g053270 25.472 424 254 10 98 468 31 445 3.05e-23 102
MS.gene059415.t1 MTR_4g064120 21.990 382 273 8 100 466 252 623 3.67e-23 103
MS.gene059415.t1 MTR_4g064180 22.674 344 242 6 138 466 289 623 4.69e-23 102
MS.gene059415.t1 MTR_7g074650 28.530 347 210 12 136 469 471 792 5.05e-23 103
MS.gene059415.t1 MTR_2g097380 24.556 338 212 8 138 468 259 560 1.40e-22 101
MS.gene059415.t1 MTR_4g095500 24.726 457 282 11 64 468 21 467 3.63e-21 96.3
MS.gene059415.t1 MTR_1g069725 25.201 373 252 10 103 468 110 462 3.07e-20 93.6
MS.gene059415.t1 MTR_1g096030 27.737 411 225 15 101 469 79 459 2.44e-19 90.9
MS.gene059415.t1 MTR_3g022830 24.083 436 237 18 103 469 26 436 4.75e-19 89.7
MS.gene059415.t1 MTR_5g015490 25.389 386 259 10 103 469 115 490 4.32e-18 87.0
MS.gene059415.t1 MTR_2g034250 29.455 275 166 10 208 469 326 585 6.01e-18 87.0
MS.gene059415.t1 MTR_4g076140 25.496 353 199 10 174 469 124 469 1.04e-17 85.9
MS.gene059415.t1 MTR_2g034260 27.758 281 174 9 202 469 320 584 1.25e-17 85.9
MS.gene059415.t1 MTR_7g109580 26.866 268 178 7 208 469 299 554 1.41e-16 82.8
MS.gene059415.t1 MTR_5g009080 23.041 434 259 11 101 469 55 478 2.26e-15 78.6
MS.gene059415.t1 MTR_4g076020 27.333 300 174 13 179 469 165 429 1.30e-14 76.3
MS.gene059415.t1 MTR_3g072710 26.115 314 189 13 174 468 210 499 1.11e-12 70.5
MS.gene059415.t1 MTR_5g058860 24.685 397 252 15 101 468 118 496 1.43e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059415.t1 AT5G52510 52.697 482 183 12 9 469 183 640 3.86e-142 422
MS.gene059415.t1 AT1G21450 37.234 470 241 13 20 469 158 593 2.81e-78 255
MS.gene059415.t1 AT2G04890 35.864 382 214 11 100 469 51 413 1.27e-63 212
MS.gene059415.t1 AT5G48150 36.317 391 208 10 100 469 120 490 1.32e-62 211
MS.gene059415.t1 AT5G48150 36.317 391 208 10 100 469 120 490 1.32e-62 211
MS.gene059415.t1 AT5G48150 36.317 391 208 10 100 469 120 490 1.32e-62 211
MS.gene059415.t1 AT5G48150 36.317 391 208 10 100 469 120 490 1.32e-62 211
MS.gene059415.t1 AT4G17230 33.247 385 227 9 100 469 156 525 4.01e-56 195
MS.gene059415.t1 AT4G17230 33.247 385 227 9 100 469 156 525 4.01e-56 195
MS.gene059415.t1 AT1G50600 33.766 385 225 9 100 469 157 526 7.98e-56 194
MS.gene059415.t1 AT1G50600 33.766 385 225 9 100 469 232 601 3.03e-55 194
MS.gene059415.t1 AT1G50600 33.766 385 225 9 100 469 228 597 3.08e-55 194
MS.gene059415.t1 AT2G01570 31.135 379 231 10 101 469 223 581 8.23e-39 149
MS.gene059415.t1 AT1G14920 29.815 379 236 10 101 469 171 529 2.76e-38 146
MS.gene059415.t1 AT3G03450 33.000 300 183 6 176 469 258 545 3.17e-37 144
MS.gene059415.t1 AT1G66350 29.187 418 263 12 64 469 110 506 1.10e-36 141
MS.gene059415.t1 AT2G29060 27.146 431 277 13 65 468 234 654 5.64e-36 141
MS.gene059415.t1 AT2G29060 27.146 431 277 13 65 468 271 691 6.09e-36 141
MS.gene059415.t1 AT5G59450 26.000 400 270 8 85 469 210 598 2.83e-35 139
MS.gene059415.t1 AT5G17490 29.973 377 237 11 103 469 157 516 5.03e-34 134
MS.gene059415.t1 AT1G07530 24.947 469 309 16 29 468 310 764 1.61e-33 134
MS.gene059415.t1 AT2G29065 26.684 386 252 12 103 468 253 627 2.87e-31 127
MS.gene059415.t1 AT3G46600 24.548 387 258 9 100 469 2 371 1.69e-29 119
MS.gene059415.t1 AT3G46600 23.282 451 306 10 36 469 120 547 4.47e-29 120
MS.gene059415.t1 AT3G46600 24.740 384 255 9 103 469 213 579 4.65e-29 120
MS.gene059415.t1 AT1G07520 23.333 390 273 10 96 468 312 692 8.01e-26 111
MS.gene059415.t1 AT1G07520 23.333 390 273 10 96 468 313 693 8.47e-26 111
MS.gene059415.t1 AT1G07520 23.333 390 273 10 96 468 148 528 9.03e-26 110
MS.gene059415.t1 AT1G07520 23.333 390 273 10 96 468 148 528 9.03e-26 110
MS.gene059415.t1 AT2G37650 24.359 390 254 11 100 469 333 701 1.18e-24 107
MS.gene059415.t1 AT3G50650 31.353 303 177 14 184 469 254 542 1.22e-24 107
MS.gene059415.t1 AT2G37650 24.359 390 254 11 100 469 345 713 1.22e-24 107
MS.gene059415.t1 AT5G66770 30.667 300 179 10 184 469 300 584 2.13e-24 106
MS.gene059415.t1 AT3G54220 30.068 296 177 12 180 468 377 649 6.64e-23 102
MS.gene059415.t1 AT3G49950 25.575 391 258 13 100 468 28 407 1.17e-21 97.4
MS.gene059415.t1 AT4G37650 26.131 398 248 12 103 469 147 529 4.58e-20 93.2
MS.gene059415.t1 AT1G50420 32.258 155 94 3 321 469 330 479 8.64e-17 83.2
MS.gene059415.t1 AT2G45160 25.683 366 241 11 117 469 292 639 8.23e-16 80.5
MS.gene059415.t1 AT1G55580 23.746 299 205 8 184 469 157 445 2.56e-15 78.2
MS.gene059415.t1 AT1G63100 27.174 276 176 11 208 468 388 653 2.47e-14 75.9
MS.gene059415.t1 AT1G63100 27.000 300 192 12 184 468 341 628 3.10e-14 75.5
MS.gene059415.t1 AT4G08250 25.806 310 194 12 176 468 186 476 1.52e-13 73.2
MS.gene059415.t1 AT3G13840 24.932 369 225 13 125 469 170 510 6.15e-12 68.2

Find 100 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGGCCCGGACCTGGGCC+TGG 0.226567 6.3:+42187712 MS.gene059415:CDS
CGAGTTGACTCGCGAGTTAT+TGG 0.228199 6.3:+42188395 MS.gene059415:CDS
GGCTGCAAATCTCGCAATTC+TGG 0.228552 6.3:+42188110 MS.gene059415:CDS
GAGACTTATCAGAATCTAAT+TGG 0.254281 6.3:+42187694 MS.gene059415:CDS
TTGTTTCAAGGTTGGTTTCA+TGG 0.293135 6.3:+42188089 MS.gene059415:CDS
CCTGTTGTTGCGGAATTGTT+CGG 0.297926 6.3:+42188021 MS.gene059415:CDS
GGCGCGTGTTGCGGAGTCTT+GGG 0.309205 6.3:+42188596 MS.gene059415:CDS
ACCTGGGCCTGGGCCTGGTC+AGG 0.310562 6.3:+42187723 MS.gene059415:CDS
CTGTTGTTGCGGAATTGTTC+GGG 0.326798 6.3:+42188022 MS.gene059415:CDS
TGGGATTTGCTTTGGTTGGA+TGG 0.327746 6.3:+42188899 MS.gene059415:CDS
TTGGGCCCGGACCTGGGCCT+GGG 0.329919 6.3:+42187713 MS.gene059415:CDS
CCCGGACCTGGGCCTGGGCC+TGG 0.330968 6.3:+42187718 MS.gene059415:CDS
GAATCTGAATTCGATGAATT+CGG 0.332765 6.3:+42187933 MS.gene059415:CDS
ACCAGGCCCAGGCCCAGGTC+CGG 0.345324 6.3:-42187719 None:intergenic
TGGCGCGTGTTGCGGAGTCT+TGG 0.359860 6.3:+42188595 MS.gene059415:CDS
GAGTCGAAAATTGTGTAATT+TGG 0.380694 6.3:+42188686 MS.gene059415:CDS
GAGTTGACTCGCGAGTTATT+GGG 0.392816 6.3:+42188396 MS.gene059415:CDS
AATCTAATTGGGCCCGGACC+TGG 0.411924 6.3:+42187706 MS.gene059415:CDS
GCCTGACCAGGCCCAGGCCC+AGG 0.417077 6.3:-42187724 None:intergenic
ATGGTGGGATTTGCTTTGGT+TGG 0.418527 6.3:+42188895 MS.gene059415:CDS
TTTCGAGTTTCGTGTTGTTT+CGG 0.418985 6.3:+42188353 MS.gene059415:CDS
ATTAAGTCGCGACTCAATAT+CGG 0.419497 6.3:+42188825 MS.gene059415:CDS
ATGGCGCGCAAGGATGAGTA+TGG 0.425941 6.3:+42188758 MS.gene059415:CDS
GGAAAACTCGGTTTGTTTCA+AGG 0.430920 6.3:+42188077 MS.gene059415:CDS
TCAAGCACAAGCTCAGAAAC+TGG 0.431854 6.3:+42187747 MS.gene059415:CDS
TCCGTTGACACACTTTCATC+AGG 0.437789 6.3:-42188474 None:intergenic
GGGATTTGCTTTGGTTGGAT+GGG 0.446689 6.3:+42188900 MS.gene059415:CDS
CAAGCGGAGAAGCTAAGAAT+TGG 0.446983 6.3:+42188330 MS.gene059415:CDS
AGCGCGTGAGGCGGCGCGTG+AGG 0.447629 6.3:+42188227 MS.gene059415:CDS
CACTCACGGTTGCTTCTGCT+TGG 0.448967 6.3:+42188928 MS.gene059415:CDS
CCAGGCCCAGGCCCAGGTCC+GGG 0.452097 6.3:-42187718 None:intergenic
ATCTAATTGGGCCCGGACCT+GGG 0.452655 6.3:+42187707 MS.gene059415:CDS
GAGAATGGTGGGATTTGCTT+TGG 0.460359 6.3:+42188891 MS.gene059415:CDS
CGCGCAAGGATGAGTATGGC+GGG 0.461869 6.3:+42188762 MS.gene059415:CDS
GCTTGTGCTTGAGCCTGACC+AGG 0.466408 6.3:-42187736 None:intergenic
GTTGAAGGTTGTTGCGGTGG+CGG 0.467280 6.3:+42188257 MS.gene059415:CDS
CCGAACAATTCCGCAACAAC+AGG 0.470616 6.3:-42188021 None:intergenic
TCAAAGGTTAGCGAATTTCA+TGG 0.473159 6.3:+42187957 MS.gene059415:CDS
GTTGAAATCGAGGATGAATC+CGG 0.473635 6.3:+42187987 MS.gene059415:CDS
CTCCTTCCTCTATCTTGACT+CGG 0.477740 6.3:-42188662 None:intergenic
GCTTGAGCCTGACCAGGCCC+AGG 0.481659 6.3:-42187730 None:intergenic
GTTGTTGCGGTGGCGGAGAA+CGG 0.484971 6.3:+42188264 MS.gene059415:CDS
AGACTTATCAGAATCTAATT+GGG 0.485068 6.3:+42187695 MS.gene059415:CDS
AACTCGGTTTGTTTCAAGGT+TGG 0.489425 6.3:+42188081 MS.gene059415:CDS
GCTCAAACCATGACCATGTC+CGG 0.491270 6.3:+42187538 None:intergenic
ATTTGGTGGCTTGTGAAGGT+AGG 0.494402 6.3:+42188703 MS.gene059415:CDS
CTCGACCGAGTCACATAAAG+AGG 0.495035 6.3:-42188628 None:intergenic
TTGCTTTAGAGATCTTGAAC+AGG 0.498596 6.3:+42187896 MS.gene059415:CDS
TCAGTTGTTGTTGGAAAACT+CGG 0.502026 6.3:+42188065 MS.gene059415:CDS
CTGTTATTACAACAAGTGAA+TGG 0.505097 6.3:+42187668 MS.gene059415:CDS
TCGAAAATTGTGTAATTTGG+TGG 0.512098 6.3:+42188689 MS.gene059415:CDS
CGCGCTCAAAGCGCGTGAGG+CGG 0.514899 6.3:+42188218 MS.gene059415:CDS
CTCGAAGATAACGACGAAGA+AGG 0.517103 6.3:+42187628 MS.gene059415:CDS
TTACTCCTCTTTATGTGACT+CGG 0.520089 6.3:+42188623 MS.gene059415:CDS
GATGACGTTGTTGATGAAGT+TGG 0.523797 6.3:-42187781 None:intergenic
GCGTGAGGGATTTAAGTTGA+AGG 0.527023 6.3:+42188242 MS.gene059415:CDS
GCGCGTGAGGCGGCGCGTGA+GGG 0.530625 6.3:+42188228 MS.gene059415:CDS
CTCTTTATGTGACTCGGTCG+AGG 0.530688 6.3:+42188629 MS.gene059415:CDS
TGAGTCGACTCAGTTGTTGT+TGG 0.533088 6.3:+42188056 MS.gene059415:CDS
TCACACTCTTCTACAAGAGT+TGG 0.538873 6.3:+42187594 MS.gene059415:CDS
TTCCTCTATCTTGACTCGGT+CGG 0.541572 6.3:-42188658 None:intergenic
TTTAGAGATCTTGAACAGGT+TGG 0.541908 6.3:+42187900 MS.gene059415:CDS
TAACGACGAAGAAGGTGAAA+CGG 0.546401 6.3:+42187636 MS.gene059415:CDS
GCGCGTGTTGCGGAGTCTTG+GGG 0.548940 6.3:+42188597 MS.gene059415:CDS
GAAGCTCGCGCCGCGTGTTG+TGG 0.551400 6.3:+42188530 MS.gene059415:CDS
GACCGAGTCAAGATAGAGGA+AGG 0.553130 6.3:+42188660 MS.gene059415:CDS
TTCCGACCGAGTCAAGATAG+AGG 0.553601 6.3:+42188656 MS.gene059415:CDS
TTTGGTGGCTTGTGAAGGTA+GGG 0.553999 6.3:+42188704 MS.gene059415:CDS
ATTCGATGAATTCGGATCAA+AGG 0.557792 6.3:+42187941 MS.gene059415:CDS
GAAGCTGCATTTGAAGAAAA+CGG 0.563799 6.3:+42188132 MS.gene059415:CDS
TGAAAATCCACCTGTTGTTG+CGG 0.565636 6.3:+42188011 MS.gene059415:CDS
TATCAGAATCTAATTGGGCC+CGG 0.565651 6.3:+42187700 MS.gene059415:CDS
TCCTGATGAAAGTGTGTCAA+CGG 0.566116 6.3:+42188473 MS.gene059415:CDS
TAGCGGTGGGTGAGATGTTG+AGG 0.568836 6.3:+42188304 MS.gene059415:CDS
AACGAGTCGCGAGTTAACTC+AGG 0.573704 6.3:+42188852 MS.gene059415:CDS
AACAATTCCGCAACAACAGG+TGG 0.579323 6.3:-42188018 None:intergenic
CTCAGTGTGAAAGAAGAGAA+TGG 0.580110 6.3:+42188876 MS.gene059415:CDS
GCTGCAAATGCGATTTCAGA+AGG 0.580818 6.3:+42187862 MS.gene059415:CDS
GTCATCAACTTCTTCATCAG+CGG 0.587327 6.3:+42187798 MS.gene059415:CDS
GTGTGAAAGAAGAGAATGGT+GGG 0.588009 6.3:+42188880 MS.gene059415:CDS
ATTTAAGTTGAAGGTTGTTG+CGG 0.588980 6.3:+42188251 MS.gene059415:CDS
TAAGTTGAAGGTTGTTGCGG+TGG 0.592167 6.3:+42188254 MS.gene059415:CDS
TGTAATTTGGTGGCTTGTGA+AGG 0.595457 6.3:+42188699 MS.gene059415:CDS
TGGTGAGAGCGTTGAAATCG+AGG 0.600312 6.3:+42187977 MS.gene059415:CDS
TCACGCGCTCAAAGCGCGTG+AGG 0.611087 6.3:+42188215 MS.gene059415:CDS
CGCCGCAAAAGTGTGTCACG+TGG 0.618368 6.3:-42188501 None:intergenic
GTTGCGGTGGCGGAGAACGG+CGG 0.627141 6.3:+42188267 MS.gene059415:CDS
AAGGTAGGGATCGTGTCGAA+AGG 0.628894 6.3:+42188718 MS.gene059415:CDS
GCCGCGTGTTGTGGTTGTTG+TGG 0.629120 6.3:+42188539 MS.gene059415:CDS
TCCACAACAACCACAACACG+CGG 0.636009 6.3:-42188540 None:intergenic
GCGCGCAAGGATGAGTATGG+CGG 0.653133 6.3:+42188761 MS.gene059415:CDS
TGAGATGTTGAGGAGTCAAG+CGG 0.659619 6.3:+42188314 MS.gene059415:CDS
TTGGATGGGAAGAACACTCA+CGG 0.668699 6.3:+42188914 MS.gene059415:CDS
GGATCGTGTCGAAAGGTGTG+AGG 0.671431 6.3:+42188725 MS.gene059415:CDS
GCGCAAGGATGAGTATGGCG+GGG 0.685055 6.3:+42188763 MS.gene059415:CDS
CGGTGATGATAGAGTGATAG+CGG 0.689852 6.3:+42188287 MS.gene059415:CDS
AGTGTGAAAGAAGAGAATGG+TGG 0.698819 6.3:+42188879 MS.gene059415:CDS
TGATGATAGAGTGATAGCGG+TGG 0.709647 6.3:+42188290 MS.gene059415:CDS
GAGTCGCGACTTAATCGACT+CGG 0.720123 6.3:-42188817 None:intergenic
GATGATAGAGTGATAGCGGT+GGG 0.720839 6.3:+42188291 MS.gene059415:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGACTTATCAGAATCTAATT+GGG + chr6.3:42187695-42187714 MS.gene059415:CDS 25.0%
!!! GTTGTTTTCTTCTATTTTAC+CGG - chr6.3:42187560-42187579 None:intergenic 25.0%
!!! TGTGTAGTTGATTTTGAAAT+TGG + chr6.3:42188165-42188184 MS.gene059415:CDS 25.0%
CTGTTATTACAACAAGTGAA+TGG + chr6.3:42187668-42187687 MS.gene059415:CDS 30.0%
GAATCTGAATTCGATGAATT+CGG + chr6.3:42187933-42187952 MS.gene059415:CDS 30.0%
GAGACTTATCAGAATCTAAT+TGG + chr6.3:42187694-42187713 MS.gene059415:CDS 30.0%
GAGTCGAAAATTGTGTAATT+TGG + chr6.3:42188686-42188705 MS.gene059415:CDS 30.0%
TCGAAAATTGTGTAATTTGG+TGG + chr6.3:42188689-42188708 MS.gene059415:CDS 30.0%
!! ATTTAAGTTGAAGGTTGTTG+CGG + chr6.3:42188251-42188270 MS.gene059415:CDS 30.0%
!!! AGTTGATTTTGAAATTGGAC+AGG + chr6.3:42188170-42188189 MS.gene059415:CDS 30.0%
!!! GTTGATTTTGAAATTGGACA+GGG + chr6.3:42188171-42188190 MS.gene059415:CDS 30.0%
ATTAAGTCGCGACTCAATAT+CGG + chr6.3:42188825-42188844 MS.gene059415:CDS 35.0%
ATTCGATGAATTCGGATCAA+AGG + chr6.3:42187941-42187960 MS.gene059415:CDS 35.0%
GAAGCTGCATTTGAAGAAAA+CGG + chr6.3:42188132-42188151 MS.gene059415:CDS 35.0%
TCAAAGGTTAGCGAATTTCA+TGG + chr6.3:42187957-42187976 MS.gene059415:CDS 35.0%
TTACTCCTCTTTATGTGACT+CGG + chr6.3:42188623-42188642 MS.gene059415:CDS 35.0%
TTTAGAGATCTTGAACAGGT+TGG + chr6.3:42187900-42187919 MS.gene059415:CDS 35.0%
! TCAGTTGTTGTTGGAAAACT+CGG + chr6.3:42188065-42188084 MS.gene059415:CDS 35.0%
! TTCTTCTATTTTACCGGACA+TGG - chr6.3:42187554-42187573 None:intergenic 35.0%
!! TGTTTTGAACAAACAGAAGC+AGG - chr6.3:42187829-42187848 None:intergenic 35.0%
!! TTGCTTTAGAGATCTTGAAC+AGG + chr6.3:42187896-42187915 MS.gene059415:CDS 35.0%
!! TTGTTTCAAGGTTGGTTTCA+TGG + chr6.3:42188089-42188108 MS.gene059415:CDS 35.0%
!! TTTCGAGTTTCGTGTTGTTT+CGG + chr6.3:42188353-42188372 MS.gene059415:CDS 35.0%
!!! TCGGTAACATTTTGACTCAT+TGG - chr6.3:42188801-42188820 None:intergenic 35.0%
AGTGTGAAAGAAGAGAATGG+TGG + chr6.3:42188879-42188898 MS.gene059415:CDS 40.0%
CTCAGTGTGAAAGAAGAGAA+TGG + chr6.3:42188876-42188895 MS.gene059415:CDS 40.0%
GTCATCAACTTCTTCATCAG+CGG + chr6.3:42187798-42187817 MS.gene059415:CDS 40.0%
GTGTGAAAGAAGAGAATGGT+GGG + chr6.3:42188880-42188899 MS.gene059415:CDS 40.0%
GTTGAAATCGAGGATGAATC+CGG + chr6.3:42187987-42188006 MS.gene059415:CDS 40.0%
TAACGACGAAGAAGGTGAAA+CGG + chr6.3:42187636-42187655 MS.gene059415:CDS 40.0%
TATCAGAATCTAATTGGGCC+CGG + chr6.3:42187700-42187719 MS.gene059415:CDS 40.0%
TCACACTCTTCTACAAGAGT+TGG + chr6.3:42187594-42187613 MS.gene059415:CDS 40.0%
TGAAAATCCACCTGTTGTTG+CGG + chr6.3:42188011-42188030 MS.gene059415:CDS 40.0%
! AACTCGGTTTGTTTCAAGGT+TGG + chr6.3:42188081-42188100 MS.gene059415:CDS 40.0%
! GATGACGTTGTTGATGAAGT+TGG - chr6.3:42187784-42187803 None:intergenic 40.0%
! GGAAAACTCGGTTTGTTTCA+AGG + chr6.3:42188077-42188096 MS.gene059415:CDS 40.0%
! TCCTGATGAAAGTGTGTCAA+CGG + chr6.3:42188473-42188492 MS.gene059415:CDS 40.0%
! TGTAATTTGGTGGCTTGTGA+AGG + chr6.3:42188699-42188718 MS.gene059415:CDS 40.0%
!! ACAACAGGTGGATTTTCAAC+CGG - chr6.3:42188009-42188028 None:intergenic 40.0%
AACAATTCCGCAACAACAGG+TGG - chr6.3:42188021-42188040 None:intergenic 45.0%
CAAGCGGAGAAGCTAAGAAT+TGG + chr6.3:42188330-42188349 MS.gene059415:CDS 45.0%
CCTGTTGTTGCGGAATTGTT+CGG + chr6.3:42188021-42188040 MS.gene059415:CDS 45.0%
CGGTGATGATAGAGTGATAG+CGG + chr6.3:42188287-42188306 MS.gene059415:CDS 45.0%
CTCCTTCCTCTATCTTGACT+CGG - chr6.3:42188665-42188684 None:intergenic 45.0%
CTCGAAGATAACGACGAAGA+AGG + chr6.3:42187628-42187647 MS.gene059415:CDS 45.0%
CTGTTGTTGCGGAATTGTTC+GGG + chr6.3:42188022-42188041 MS.gene059415:CDS 45.0%
GCTGCAAATGCGATTTCAGA+AGG + chr6.3:42187862-42187881 MS.gene059415:CDS 45.0%
TCAAGCACAAGCTCAGAAAC+TGG + chr6.3:42187747-42187766 MS.gene059415:CDS 45.0%
TCCGTTGACACACTTTCATC+AGG - chr6.3:42188477-42188496 None:intergenic 45.0%
TGAGATGTTGAGGAGTCAAG+CGG + chr6.3:42188314-42188333 MS.gene059415:CDS 45.0%
TTGGATGGGAAGAACACTCA+CGG + chr6.3:42188914-42188933 MS.gene059415:CDS 45.0%
! ATCCACGTGACACACTTTTG+CGG + chr6.3:42188499-42188518 MS.gene059415:CDS 45.0%
! ATGGTGGGATTTGCTTTGGT+TGG + chr6.3:42188895-42188914 MS.gene059415:CDS 45.0%
! ATTTGGTGGCTTGTGAAGGT+AGG + chr6.3:42188703-42188722 MS.gene059415:CDS 45.0%
! GAGAATGGTGGGATTTGCTT+TGG + chr6.3:42188891-42188910 MS.gene059415:CDS 45.0%
! GGGATTTGCTTTGGTTGGAT+GGG + chr6.3:42188900-42188919 MS.gene059415:CDS 45.0%
! TAAGTTGAAGGTTGTTGCGG+TGG + chr6.3:42188254-42188273 MS.gene059415:CDS 45.0%
! TGAGTCGACTCAGTTGTTGT+TGG + chr6.3:42188056-42188075 MS.gene059415:CDS 45.0%
! TGGGATTTGCTTTGGTTGGA+TGG + chr6.3:42188899-42188918 MS.gene059415:CDS 45.0%
! TTTGGTGGCTTGTGAAGGTA+GGG + chr6.3:42188704-42188723 MS.gene059415:CDS 45.0%
!! GAGTTGACTCGCGAGTTATT+GGG + chr6.3:42188396-42188415 MS.gene059415:CDS 45.0%
!! GATGATAGAGTGATAGCGGT+GGG + chr6.3:42188291-42188310 MS.gene059415:CDS 45.0%
!! GCGTGAGGGATTTAAGTTGA+AGG + chr6.3:42188242-42188261 MS.gene059415:CDS 45.0%
!! GTCGAAAGGTGTGAGGTTTT+TGG + chr6.3:42188732-42188751 MS.gene059415:CDS 45.0%
!! TGATGATAGAGTGATAGCGG+TGG + chr6.3:42188290-42188309 MS.gene059415:CDS 45.0%
!! TTCCTCTATCTTGACTCGGT+CGG - chr6.3:42188661-42188680 None:intergenic 45.0%
!! TTGTAATACCGCGCCGTTTT+TGG + chr6.3:42188575-42188594 MS.gene059415:CDS 45.0%
!!! GGTGTGAGGTTTTTGGCAAA+TGG + chr6.3:42188739-42188758 MS.gene059415:CDS 45.0%
AACGAGTCGCGAGTTAACTC+AGG + chr6.3:42188852-42188871 MS.gene059415:CDS 50.0%
AAGGTAGGGATCGTGTCGAA+AGG + chr6.3:42188718-42188737 MS.gene059415:CDS 50.0%
CCGAACAATTCCGCAACAAC+AGG - chr6.3:42188024-42188043 None:intergenic 50.0%
CTCGACCGAGTCACATAAAG+AGG - chr6.3:42188631-42188650 None:intergenic 50.0%
GACCGAGTCAAGATAGAGGA+AGG + chr6.3:42188660-42188679 MS.gene059415:CDS 50.0%
GAGTCGCGACTTAATCGACT+CGG - chr6.3:42188820-42188839 None:intergenic 50.0%
GGCTGCAAATCTCGCAATTC+TGG + chr6.3:42188110-42188129 MS.gene059415:CDS 50.0%
TCCACAACAACCACAACACG+CGG - chr6.3:42188543-42188562 None:intergenic 50.0%
TTCCGACCGAGTCAAGATAG+AGG + chr6.3:42188656-42188675 MS.gene059415:CDS 50.0%
!! CGAGTTGACTCGCGAGTTAT+TGG + chr6.3:42188395-42188414 MS.gene059415:CDS 50.0%
!! CTCTTTATGTGACTCGGTCG+AGG + chr6.3:42188629-42188648 MS.gene059415:CDS 50.0%
!! TGGTGAGAGCGTTGAAATCG+AGG + chr6.3:42187977-42187996 MS.gene059415:CDS 50.0%
!! TTTTTGGCAAATGGCGCGCA+AGG + chr6.3:42188748-42188767 MS.gene059415:CDS 50.0%
AATCTAATTGGGCCCGGACC+TGG + chr6.3:42187706-42187725 MS.gene059415:CDS 55.0%
ATCTAATTGGGCCCGGACCT+GGG + chr6.3:42187707-42187726 MS.gene059415:CDS 55.0%
ATGGCGCGCAAGGATGAGTA+TGG + chr6.3:42188758-42188777 MS.gene059415:CDS 55.0%
CACTCACGGTTGCTTCTGCT+TGG + chr6.3:42188928-42188947 MS.gene059415:CDS 55.0%
GTTGAAGGTTGTTGCGGTGG+CGG + chr6.3:42188257-42188276 MS.gene059415:CDS 55.0%
TAGCGGTGGGTGAGATGTTG+AGG + chr6.3:42188304-42188323 MS.gene059415:CDS 55.0%
TCCGCAACACGCGCCAAAAA+CGG - chr6.3:42188591-42188610 None:intergenic 55.0%
! GGATCGTGTCGAAAGGTGTG+AGG + chr6.3:42188725-42188744 MS.gene059415:CDS 55.0%
AACACGCGCCAAAAACGGCG+CGG - chr6.3:42188586-42188605 None:intergenic 60.0%
CGCGCAAGGATGAGTATGGC+GGG + chr6.3:42188762-42188781 MS.gene059415:CDS 60.0%
GCCGCGTGTTGTGGTTGTTG+TGG + chr6.3:42188539-42188558 MS.gene059415:CDS 60.0%
GCGCAAGGATGAGTATGGCG+GGG + chr6.3:42188763-42188782 MS.gene059415:CDS 60.0%
GCGCGCAAGGATGAGTATGG+CGG + chr6.3:42188761-42188780 MS.gene059415:CDS 60.0%
GCTTGTGCTTGAGCCTGACC+AGG - chr6.3:42187739-42187758 None:intergenic 60.0%
! CGCCGCAAAAGTGTGTCACG+TGG - chr6.3:42188504-42188523 None:intergenic 60.0%
!! GTTGTTGCGGTGGCGGAGAA+CGG + chr6.3:42188264-42188283 MS.gene059415:CDS 60.0%
!!! GCCGTTTTTGGCGCGTGTTG+CGG + chr6.3:42188587-42188606 MS.gene059415:CDS 60.0%
GCGCGTGTTGCGGAGTCTTG+GGG + chr6.3:42188597-42188616 MS.gene059415:CDS 65.0%
GGCGCGTGTTGCGGAGTCTT+GGG + chr6.3:42188596-42188615 MS.gene059415:CDS 65.0%
TCACGCGCTCAAAGCGCGTG+AGG + chr6.3:42188215-42188234 MS.gene059415:CDS 65.0%
TGGCGCGTGTTGCGGAGTCT+TGG + chr6.3:42188595-42188614 MS.gene059415:CDS 65.0%
CGCGCTCAAAGCGCGTGAGG+CGG + chr6.3:42188218-42188237 MS.gene059415:CDS 70.0%
GAAGCTCGCGCCGCGTGTTG+TGG + chr6.3:42188530-42188549 MS.gene059415:CDS 70.0%
GCTTGAGCCTGACCAGGCCC+AGG - chr6.3:42187733-42187752 None:intergenic 70.0%
!! GTTGCGGTGGCGGAGAACGG+CGG + chr6.3:42188267-42188286 MS.gene059415:CDS 70.0%
ACCAGGCCCAGGCCCAGGTC+CGG - chr6.3:42187722-42187741 None:intergenic 75.0%
ACCTGGGCCTGGGCCTGGTC+AGG + chr6.3:42187723-42187742 MS.gene059415:CDS 75.0%
ATTGGGCCCGGACCTGGGCC+TGG + chr6.3:42187712-42187731 MS.gene059415:CDS 75.0%
TTGGGCCCGGACCTGGGCCT+GGG + chr6.3:42187713-42187732 MS.gene059415:CDS 75.0%
! AGCGCGTGAGGCGGCGCGTG+AGG + chr6.3:42188227-42188246 MS.gene059415:CDS 80.0%
! CCAGGCCCAGGCCCAGGTCC+GGG - chr6.3:42187721-42187740 None:intergenic 80.0%
! GCCTGACCAGGCCCAGGCCC+AGG - chr6.3:42187727-42187746 None:intergenic 80.0%
! GCGCGTGAGGCGGCGCGTGA+GGG + chr6.3:42188228-42188247 MS.gene059415:CDS 80.0%
! CCCGGACCTGGGCCTGGGCC+TGG + chr6.3:42187718-42187737 MS.gene059415:CDS 85.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 42187547 42188956 42187547 ID=MS.gene059415
chr6.3 mRNA 42187547 42188956 42187547 ID=MS.gene059415.t1;Parent=MS.gene059415
chr6.3 exon 42187547 42188956 42187547 ID=MS.gene059415.t1.exon1;Parent=MS.gene059415.t1
chr6.3 CDS 42187547 42188956 42187547 ID=cds.MS.gene059415.t1;Parent=MS.gene059415.t1
Gene Sequence

>MS.gene059415

ATGACCATGTCCGGTAAAATAGAAGAAAACAACGTCAATAACAACGATCACACTCTTCTACAAGAGTTGGAGAAGCAGCTTCTCGAAGATAACGACGAAGAAGGTGAAACGGAAACAGCTTCTGTTATTACAACAAGTGAATGGTCAGAGACTTATCAGAATCTAATTGGGCCCGGACCTGGGCCTGGGCCTGGTCAGGCTCAAGCACAAGCTCAGAAACTGGTATCTTCATCACCAACTTCATCAACAACGTCATCAACTTCTTCATCAGCGGCTTCACCTGCTTCTGTTTGTTCAAAACAAACGCTAATTGAAGCTGCAAATGCGATTTCAGAAGGTAAAAATGATGTTGCTTTAGAGATCTTGAACAGGTTGGTTCAGAATTTGAATCTGAATTCGATGAATTCGGATCAAAGGTTAGCGAATTTCATGGTGAGAGCGTTGAAATCGAGGATGAATCCGGTTGAAAATCCACCTGTTGTTGCGGAATTGTTCGGGAGAGAACATGCTGAGTCGACTCAGTTGTTGTTGGAAAACTCGGTTTGTTTCAAGGTTGGTTTCATGGCTGCAAATCTCGCAATTCTGGAAGCTGCATTTGAAGAAAACGGTAACAGTTTCTGTGTAGTTGATTTTGAAATTGGACAGGGAAAACAGTATGTGAATCTTCTTCACGCGCTCAAAGCGCGTGAGGCGGCGCGTGAGGGATTTAAGTTGAAGGTTGTTGCGGTGGCGGAGAACGGCGGTGATGATAGAGTGATAGCGGTGGGTGAGATGTTGAGGAGTCAAGCGGAGAAGCTAAGAATTGGTTTCGAGTTTCGTGTTGTTTCGGTGTCTCAGAGACAAGTCTACGAGTTGACTCGCGAGTTATTGGGATGTGATGTTGAAGAGACAGTAATTGTGAATTTCGCGTATAAGCTGAATAGAATTCCTGATGAAAGTGTGTCAACGGAGAATCCACGTGACACACTTTTGCGGCGCGTGAAGAAGCTCGCGCCGCGTGTTGTGGTTGTTGTGGAGCAAGAAATGAATTGTAATACCGCGCCGTTTTTGGCGCGTGTTGCGGAGTCTTGGGGTTATTACTCCTCTTTATGTGACTCGGTCGAGGAAAATTCCGACCGAGTCAAGATAGAGGAAGGAGTGAGTCGAAAATTGTGTAATTTGGTGGCTTGTGAAGGTAGGGATCGTGTCGAAAGGTGTGAGGTTTTTGGCAAATGGCGCGCAAGGATGAGTATGGCGGGGTTTAAGATGAAACCAATGAGTCAAAATGTTACCGAGTCGATTAAGTCGCGACTCAATATCGGTAATAACGAGTCGCGAGTTAACTCAGGACTCAGTGTGAAAGAAGAGAATGGTGGGATTTGCTTTGGTTGGATGGGAAGAACACTCACGGTTGCTTCTGCTTGGCGTTAA
Protein sequence

>MS.gene059415.t1

MTMSGKIEENNVNNNDHTLLQELEKQLLEDNDEEGETETASVITTSEWSETYQNLIGPGPGPGPGQAQAQAQKLVSSSPTSSTTSSTSSSAASPASVCSKQTLIEAANAISEGKNDVALEILNRLVQNLNLNSMNSDQRLANFMVRALKSRMNPVENPPVVAELFGREHAESTQLLLENSVCFKVGFMAANLAILEAAFEENGNSFCVVDFEIGQGKQYVNLLHALKAREAAREGFKLKVVAVAENGGDDRVIAVGEMLRSQAEKLRIGFEFRVVSVSQRQVYELTRELLGCDVEETVIVNFAYKLNRIPDESVSTENPRDTLLRRVKKLAPRVVVVVEQEMNCNTAPFLARVAESWGYYSSLCDSVEENSDRVKIEEGVSRKLCNLVACEGRDRVERCEVFGKWRARMSMAGFKMKPMSQNVTESIKSRLNIGNNESRVNSGLSVKEENGGICFGWMGRTLTVASAWR