Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059589.t1 | XP_003620577.2 | 70.2 | 84 | 25 | 0 | 1 | 84 | 293 | 376 | 6.90E-25 | 123.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059589.t1 | G7KNZ9 | 70.2 | 84 | 25 | 0 | 1 | 84 | 293 | 376 | 5.0e-25 | 123.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059589 | MS.gene47049 | 0.809081 | 2.26E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059589.t1 | MTR_6g087150 | 90.769 | 65 | 6 | 0 | 1 | 65 | 293 | 357 | 1.02e-36 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAAGAAGGAGGAAGCTT+TGG | 0.319725 | 6.4:+61177029 | MS.gene059589:CDS |
| TCAGCTGTTCTTCTTGCAAT+TGG | 0.337125 | 6.4:-61177055 | None:intergenic |
| ACTCCACGCGGTGGGAGCCT+TGG | 0.440403 | 6.4:+61177390 | MS.gene059589:CDS |
| TCTTGAAAATAATCTTAAAG+AGG | 0.469161 | 6.4:+61177009 | MS.gene059589:CDS |
| GATTATCCTTGAGGTTCAGC+AGG | 0.476960 | 6.4:+61177182 | MS.gene059589:CDS |
| ATAATCTTAAAGAGGAAAGA+AGG | 0.482860 | 6.4:+61177017 | MS.gene059589:CDS |
| CTCCCACCGCGTGGAGTCTG+AGG | 0.523289 | 6.4:-61177384 | None:intergenic |
| TTGCAAGAAGAACAGCTGAA+TGG | 0.523336 | 6.4:+61177059 | MS.gene059589:CDS |
| CTCTGACCTGCTGAACCTCA+AGG | 0.544207 | 6.4:-61177188 | None:intergenic |
| AGATCAGTGTCAGAAATCAC+TGG | 0.561549 | 6.4:+61176929 | MS.gene059589:CDS |
| AAACTGTGCTCGAGTGTCCA+AGG | 0.585029 | 6.4:-61177407 | None:intergenic |
| CTCCTCAGACTCCACGCGGT+GGG | 0.586815 | 6.4:+61177382 | MS.gene059589:CDS |
| CCTCCTCAGACTCCACGCGG+TGG | 0.601133 | 6.4:+61177381 | MS.gene059589:CDS |
| ATCTTAAAGAGGAAAGAAGG+AGG | 0.605571 | 6.4:+61177020 | MS.gene059589:CDS |
| CCACCGCGTGGAGTCTGAGG+AGG | 0.614425 | 6.4:-61177381 | None:intergenic |
| TGTCCAAGGCTCCCACCGCG+TGG | 0.644858 | 6.4:-61177393 | None:intergenic |
| AACTCGTAGAATGCTTACAG+AGG | 0.693639 | 6.4:+61177221 | MS.gene059589:CDS |
| TATCCTCCTCAGACTCCACG+CGG | 0.752468 | 6.4:+61177378 | MS.gene059589:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAAAATTCAAGATTATGA+TGG | + | chr6.4:61176899-61176918 | MS.gene059589:CDS | 20.0% |
| !! | TCATATCTAGAAATTAAAGT+TGG | - | chr6.4:61177340-61177359 | None:intergenic | 20.0% |
| !! | TCTTGAAAATAATCTTAAAG+AGG | + | chr6.4:61177009-61177028 | MS.gene059589:CDS | 20.0% |
| !!! | AACTTTAATTTCTAGATATG+AGG | + | chr6.4:61177339-61177358 | MS.gene059589:intron | 20.0% |
| ! | ATAATCTTAAAGAGGAAAGA+AGG | + | chr6.4:61177017-61177036 | MS.gene059589:CDS | 25.0% |
| !!! | AGATTTTGACATGAAGAAAA+GGG | + | chr6.4:61176967-61176986 | MS.gene059589:CDS | 25.0% |
| !!! | TTCTTTAGGTACTTACTTTT+AGG | + | chr6.4:61177160-61177179 | MS.gene059589:intron | 25.0% |
| GGTTTGTCAATAGAGAAAAA+AGG | + | chr6.4:61177080-61177099 | MS.gene059589:intron | 30.0% | |
| GTACCTAAAGAAAGTGAAAA+TGG | - | chr6.4:61177152-61177171 | None:intergenic | 30.0% | |
| ! | AATACATGATTTGTACAACG+AGG | + | chr6.4:61177246-61177265 | MS.gene059589:intron | 30.0% |
| ! | AGTCCATTTTCACTTTCTTT+AGG | + | chr6.4:61177146-61177165 | MS.gene059589:intron | 30.0% |
| !! | GAGATTTTGACATGAAGAAA+AGG | + | chr6.4:61176966-61176985 | MS.gene059589:CDS | 30.0% |
| !!! | TACTTTTAGGATTATCCTTG+AGG | + | chr6.4:61177173-61177192 | MS.gene059589:intron | 30.0% |
| ATCTTAAAGAGGAAAGAAGG+AGG | + | chr6.4:61177020-61177039 | MS.gene059589:CDS | 35.0% | |
| ! | GTAAGCATTCTACGAGTTTT+AGG | - | chr6.4:61177219-61177238 | None:intergenic | 35.0% |
| AACTCGTAGAATGCTTACAG+AGG | + | chr6.4:61177221-61177240 | MS.gene059589:CDS | 40.0% | |
| AGATCAGTGTCAGAAATCAC+TGG | + | chr6.4:61176929-61176948 | MS.gene059589:CDS | 40.0% | |
| TCAGCTGTTCTTCTTGCAAT+TGG | - | chr6.4:61177058-61177077 | None:intergenic | 40.0% | |
| TGAGCTGAATTCTTATCGAC+TGG | + | chr6.4:61177313-61177332 | MS.gene059589:intron | 40.0% | |
| TTGCAAGAAGAACAGCTGAA+TGG | + | chr6.4:61177059-61177078 | MS.gene059589:CDS | 40.0% | |
| AGGAAAGAAGGAGGAAGCTT+TGG | + | chr6.4:61177029-61177048 | MS.gene059589:CDS | 45.0% | |
| GATTATCCTTGAGGTTCAGC+AGG | + | chr6.4:61177182-61177201 | MS.gene059589:CDS | 45.0% | |
| AAACTGTGCTCGAGTGTCCA+AGG | - | chr6.4:61177410-61177429 | None:intergenic | 50.0% | |
| CTCTGACCTGCTGAACCTCA+AGG | - | chr6.4:61177191-61177210 | None:intergenic | 55.0% | |
| TATCCTCCTCAGACTCCACG+CGG | + | chr6.4:61177378-61177397 | MS.gene059589:CDS | 55.0% | |
| CTCCTCAGACTCCACGCGGT+GGG | + | chr6.4:61177382-61177401 | MS.gene059589:CDS | 65.0% | |
| ACTCCACGCGGTGGGAGCCT+TGG | + | chr6.4:61177390-61177409 | MS.gene059589:CDS | 70.0% | |
| CCACCGCGTGGAGTCTGAGG+AGG | - | chr6.4:61177384-61177403 | None:intergenic | 70.0% | |
| CCTCCTCAGACTCCACGCGG+TGG | + | chr6.4:61177381-61177400 | MS.gene059589:CDS | 70.0% | |
| CTCCCACCGCGTGGAGTCTG+AGG | - | chr6.4:61177387-61177406 | None:intergenic | 70.0% | |
| TGTCCAAGGCTCCCACCGCG+TGG | - | chr6.4:61177396-61177415 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 61176893 | 61177431 | 61176893 | ID=MS.gene059589 |
| chr6.4 | mRNA | 61176893 | 61177431 | 61176893 | ID=MS.gene059589.t1;Parent=MS.gene059589 |
| chr6.4 | exon | 61176893 | 61177080 | 61176893 | ID=MS.gene059589.t1.exon1;Parent=MS.gene059589.t1 |
| chr6.4 | CDS | 61176893 | 61177080 | 61176893 | ID=cds.MS.gene059589.t1;Parent=MS.gene059589.t1 |
| chr6.4 | exon | 61177182 | 61177242 | 61177182 | ID=MS.gene059589.t1.exon2;Parent=MS.gene059589.t1 |
| chr6.4 | CDS | 61177182 | 61177242 | 61177182 | ID=cds.MS.gene059589.t1;Parent=MS.gene059589.t1 |
| chr6.4 | exon | 61177366 | 61177431 | 61177366 | ID=MS.gene059589.t1.exon3;Parent=MS.gene059589.t1 |
| chr6.4 | CDS | 61177366 | 61177431 | 61177366 | ID=cds.MS.gene059589.t1;Parent=MS.gene059589.t1 |
| Gene Sequence |
| Protein sequence |
