Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059963.t1 | KEH31741.1 | 99 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 1.20E-45 | 192.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059963.t1 | Q8L7N3 | 66.7 | 99 | 33 | 0 | 5 | 103 | 6 | 104 | 4.8e-27 | 121.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059963.t1 | A0A072UQV7 | 99.0 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 8.6e-46 | 192.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059963 | MS.gene07407 | 0.803987 | 2.68E-49 | -1.69E-46 |
| MS.gene059963 | MS.gene48298 | 0.910709 | 1.38E-82 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059963.t1 | MTR_4g104430 | 99.029 | 103 | 1 | 0 | 1 | 103 | 1 | 103 | 1.42e-70 | 205 |
| MS.gene059963.t1 | MTR_4g104430 | 98.876 | 89 | 1 | 0 | 15 | 103 | 1 | 89 | 4.21e-59 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059963.t1 | AT1G78790 | 66.667 | 99 | 33 | 0 | 5 | 103 | 6 | 104 | 1.80e-40 | 129 |
| MS.gene059963.t1 | AT1G78790 | 66.667 | 99 | 33 | 0 | 5 | 103 | 6 | 104 | 1.80e-40 | 129 |
| MS.gene059963.t1 | AT1G78790 | 66.667 | 99 | 33 | 0 | 5 | 103 | 6 | 104 | 1.80e-40 | 129 |
| MS.gene059963.t1 | AT1G78790 | 53.535 | 99 | 28 | 1 | 5 | 103 | 6 | 86 | 1.60e-25 | 91.7 |
Find 21 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCCATCATGAAGCGTATT+TGG | 0.181659 | 8.1:-20040144 | MS.gene059963:CDS |
| AGAAGTTGTCCGATTCTTCT+TGG | 0.300405 | 8.1:+20039228 | None:intergenic |
| TCAAATTGAAGCTACTCATT+TGG | 0.368792 | 8.1:-20038859 | MS.gene059963:CDS |
| TGTTGCAACCAATGATGCTT+TGG | 0.379913 | 8.1:-20040004 | MS.gene059963:CDS |
| AAGAAGAATCGGACAACTTC+TGG | 0.380642 | 8.1:-20039226 | MS.gene059963:intron |
| AGCTTACCTGTGAACTTCTC+CGG | 0.383589 | 8.1:-20039046 | MS.gene059963:CDS |
| CTCGGAATCCAAAGCATCAT+TGG | 0.387476 | 8.1:+20039996 | None:intergenic |
| ATTTGGACATTGCGAACCTT+AGG | 0.435108 | 8.1:-20040127 | MS.gene059963:intron |
| CTGGATCGTCCAAGAAGAAT+CGG | 0.493954 | 8.1:-20039237 | MS.gene059963:CDS |
| GCTTACCTGTGAACTTCTCC+GGG | 0.512267 | 8.1:-20039045 | MS.gene059963:CDS |
| CAATGATGCTTTGGATTCCG+AGG | 0.519977 | 8.1:-20039995 | MS.gene059963:intron |
| TTACTGCTACAGGTAGCAGC+TGG | 0.525226 | 8.1:-20039256 | MS.gene059963:intron |
| AGCCGTACAGATTCGAAGGT+TGG | 0.537616 | 8.1:+20040262 | None:intergenic |
| TGTCCAAATACGCTTCATGA+TGG | 0.552980 | 8.1:+20040141 | None:intergenic |
| ACAGGTAAGCTTCAAAGCAC+TGG | 0.559640 | 8.1:+20039058 | None:intergenic |
| AAATACGCTTCATGATGGAA+TGG | 0.583453 | 8.1:+20040146 | None:intergenic |
| GGATAGCCGTACAGATTCGA+AGG | 0.605653 | 8.1:+20040258 | None:intergenic |
| TATTGCTATTGCAGAGACTG+AGG | 0.624787 | 8.1:-20038889 | MS.gene059963:CDS |
| TAGAAATCTAAAAGCAACTG+AGG | 0.627717 | 8.1:+20038825 | None:intergenic |
| ATTGCTATTGCAGAGACTGA+GGG | 0.649753 | 8.1:-20038888 | MS.gene059963:CDS |
| ATTGCAGAGACTGAGGGTGA+TGG | 0.654914 | 8.1:-20038882 | MS.gene059963:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGCTAAAATATTTAATGT+AGG | + | chr8.1:20039748-20039767 | None:intergenic | 15.0% |
| !! | TAGCTAAAATATTTAATGTA+GGG | + | chr8.1:20039747-20039766 | None:intergenic | 15.0% |
| !!! | TCTCTTTTAAAGTTTAATAT+GGG | + | chr8.1:20040093-20040112 | None:intergenic | 15.0% |
| !!! | TTCTCTTTTAAAGTTTAATA+TGG | + | chr8.1:20040094-20040113 | None:intergenic | 15.0% |
| !! | AACAAAAAATTTGAAAACCT+CGG | + | chr8.1:20039120-20039139 | None:intergenic | 20.0% |
| !! | ACAATATTGTAGTTTATAAC+TGG | - | chr8.1:20039676-20039695 | MS.gene059963:intron | 20.0% |
| !! | ATGAAGAAAAAAGATTGAAA+TGG | + | chr8.1:20039587-20039606 | None:intergenic | 20.0% |
| !! | ATTTAATGTAGGGAAAATTA+AGG | + | chr8.1:20039737-20039756 | None:intergenic | 20.0% |
| !! | TTTAGTGTATGCTTTATAAA+TGG | - | chr8.1:20039321-20039340 | MS.gene059963:intron | 20.0% |
| !!! | AGATAAAGTTTTCTGTAAAA+TGG | + | chr8.1:20039656-20039675 | None:intergenic | 20.0% |
| !!! | ATCTTTTTTCTTCATTACTT+AGG | - | chr8.1:20039592-20039611 | MS.gene059963:intron | 20.0% |
| !!! | TATTGCAACATTTGTTTTTA+TGG | - | chr8.1:20039615-20039634 | MS.gene059963:intron | 20.0% |
| ! | ATTGTAGTTTATAACTGGTT+GGG | - | chr8.1:20039681-20039700 | MS.gene059963:intron | 25.0% |
| ! | CAATTTCTTCATTCAATAAC+AGG | - | chr8.1:20038919-20038938 | MS.gene059963:intron | 25.0% |
| ! | GGTTAATTAAGAAATTTGTG+TGG | - | chr8.1:20039276-20039295 | MS.gene059963:intron | 25.0% |
| ! | TATTGTAGTTTATAACTGGT+TGG | - | chr8.1:20039680-20039699 | MS.gene059963:intron | 25.0% |
| AAGAAAATTAGCATGAAGAG+TGG | + | chr8.1:20038885-20038904 | None:intergenic | 30.0% | |
| ACAGAAAGGTTATGTCAATA+TGG | + | chr8.1:20040162-20040181 | None:intergenic | 30.0% | |
| AGATTGAAATGGAAACAGTT+TGG | + | chr8.1:20039576-20039595 | None:intergenic | 30.0% | |
| ATCTCCACCAAATAAGAAAT+CGG | + | chr8.1:20039921-20039940 | None:intergenic | 30.0% | |
| ATTAGAGAAGAAAGGGTTAT+AGG | + | chr8.1:20038861-20038880 | None:intergenic | 30.0% | |
| ATTGACATGAATATCATCTC+AGG | + | chr8.1:20039198-20039217 | None:intergenic | 30.0% | |
| GCAACGATTAAGAATTAAGT+AGG | + | chr8.1:20039796-20039815 | None:intergenic | 30.0% | |
| GTATGCTTTATAAATGGAAG+CGG | - | chr8.1:20039327-20039346 | MS.gene059963:intron | 30.0% | |
| TAGAAATCTAAAAGCAACTG+AGG | + | chr8.1:20040273-20040292 | None:intergenic | 30.0% | |
| TATGCTTTATAAATGGAAGC+GGG | - | chr8.1:20039328-20039347 | MS.gene059963:intron | 30.0% | |
| TCAAATTGAAGCTACTCATT+TGG | - | chr8.1:20040236-20040255 | MS.gene059963:intron | 30.0% | |
| !! | ATGCTACTTGTTGTTGAATT+CGG | - | chr8.1:20039224-20039243 | MS.gene059963:intron | 30.0% |
| !!! | GGATTTTGGGTTAAGAAATT+TGG | - | chr8.1:20039247-20039266 | MS.gene059963:CDS | 30.0% |
| AAGGTTATGTCAATATGGCA+TGG | + | chr8.1:20040157-20040176 | None:intergenic | 35.0% | |
| AGAGTGGAATTAGAGAAGAA+AGG | + | chr8.1:20038869-20038888 | None:intergenic | 35.0% | |
| ATTCCATCATGAAGCGTATT+TGG | - | chr8.1:20038951-20038970 | MS.gene059963:intron | 35.0% | |
| CTGTTGTCCGATTTCTTATT+TGG | - | chr8.1:20039911-20039930 | MS.gene059963:intron | 35.0% | |
| GAGTGGAATTAGAGAAGAAA+GGG | + | chr8.1:20038868-20038887 | None:intergenic | 35.0% | |
| GGTTAAGAAATTTGGCTAAC+AGG | - | chr8.1:20039255-20039274 | MS.gene059963:intron | 35.0% | |
| TTAAGAAATTTGTGTGGCCT+TGG | - | chr8.1:20039282-20039301 | MS.gene059963:intron | 35.0% | |
| ! | AAATACGCTTCATGATGGAA+TGG | + | chr8.1:20038952-20038971 | None:intergenic | 35.0% |
| ! | TTGTCCGATTTCTTATTTGG+TGG | - | chr8.1:20039914-20039933 | MS.gene059963:intron | 35.0% |
| ! | TTGTTGAATTCGGGGATTTT+GGG | - | chr8.1:20039234-20039253 | MS.gene059963:CDS | 35.0% |
| !! | CACGCTATGTGTTTGTTATT+TGG | - | chr8.1:20039161-20039180 | MS.gene059963:intron | 35.0% |
| !! | TGCTACTTGTTGTTGAATTC+GGG | - | chr8.1:20039225-20039244 | MS.gene059963:intron | 35.0% |
| AACCAACCTTCGAATCTGTA+CGG | - | chr8.1:20038831-20038850 | MS.gene059963:CDS | 40.0% | |
| AAGAAGAATCGGACAACTTC+TGG | - | chr8.1:20039869-20039888 | MS.gene059963:intron | 40.0% | |
| ACACTGACACTTCAGATTGA+AGG | - | chr8.1:20039439-20039458 | MS.gene059963:intron | 40.0% | |
| AGAAGTTGTCCGATTCTTCT+TGG | + | chr8.1:20039870-20039889 | None:intergenic | 40.0% | |
| ATAACACCGACACTTCAGAT+TGG | - | chr8.1:20039370-20039389 | MS.gene059963:intron | 40.0% | |
| ATGTCACACTGTAGTTGTCT+TGG | - | chr8.1:20039955-20039974 | MS.gene059963:intron | 40.0% | |
| ATTAGCTGCATGGGAAAAGA+CGG | + | chr8.1:20040016-20040035 | None:intergenic | 40.0% | |
| ATTTGGACATTGCGAACCTT+AGG | - | chr8.1:20038968-20038987 | MS.gene059963:intron | 40.0% | |
| GCACTGTCTGTATTGCAAAT+GGG | - | chr8.1:20039814-20039833 | MS.gene059963:intron | 40.0% | |
| GTGAACCTGTAACAAAAACC+CGG | + | chr8.1:20040071-20040090 | None:intergenic | 40.0% | |
| GTTGTCTTGGCATTGCAAAT+TGG | - | chr8.1:20039968-20039987 | MS.gene059963:intron | 40.0% | |
| TAACCTTTCTGTTGATGCAG+AGG | - | chr8.1:20040170-20040189 | MS.gene059963:intron | 40.0% | |
| TGCACTGTCTGTATTGCAAA+TGG | - | chr8.1:20039813-20039832 | MS.gene059963:intron | 40.0% | |
| TGTCCAAATACGCTTCATGA+TGG | + | chr8.1:20038957-20038976 | None:intergenic | 40.0% | |
| TGTTGCAACCAATGATGCTT+TGG | - | chr8.1:20039091-20039110 | MS.gene059963:intron | 40.0% | |
| ! | AAGAGAAAGTGTGTGACCTA+AGG | + | chr8.1:20038987-20039006 | None:intergenic | 40.0% |
| ! | ACCTAACTCTAAAAGCTCCA+AGG | + | chr8.1:20039302-20039321 | None:intergenic | 40.0% |
| ! | ATTGCTATTGCAGAGACTGA+GGG | - | chr8.1:20040207-20040226 | MS.gene059963:intron | 40.0% |
| ! | CTGAAGTGTCAGTGTAACAT+GGG | + | chr8.1:20039434-20039453 | None:intergenic | 40.0% |
| ! | GTAACTACATGTGTCGCTAT+CGG | + | chr8.1:20039488-20039507 | None:intergenic | 40.0% |
| ! | GTTGTTGAATTCGGGGATTT+TGG | - | chr8.1:20039233-20039252 | MS.gene059963:CDS | 40.0% |
| ! | TATTGCTATTGCAGAGACTG+AGG | - | chr8.1:20040206-20040225 | MS.gene059963:intron | 40.0% |
| ! | TCTGAAGTGTCAGTGTAACA+TGG | + | chr8.1:20039435-20039454 | None:intergenic | 40.0% |
| !! | CAAATGGGTTTTACTGCTAC+AGG | - | chr8.1:20039829-20039848 | MS.gene059963:intron | 40.0% |
| !! | GCTACTTGTTGTTGAATTCG+GGG | - | chr8.1:20039226-20039245 | MS.gene059963:intron | 40.0% |
| AAAAACCCGGAGAAGTTCAC+AGG | + | chr8.1:20040058-20040077 | None:intergenic | 45.0% | |
| AACTGACACACACTCGTACA+TGG | + | chr8.1:20039553-20039572 | None:intergenic | 45.0% | |
| ACAGGTAAGCTTCAAAGCAC+TGG | + | chr8.1:20040040-20040059 | None:intergenic | 45.0% | |
| AGCTTACCTGTGAACTTCTC+CGG | - | chr8.1:20040049-20040068 | MS.gene059963:intron | 45.0% | |
| CTGGATCGTCCAAGAAGAAT+CGG | - | chr8.1:20039858-20039877 | MS.gene059963:intron | 45.0% | |
| ! | CAATGATGCTTTGGATTCCG+AGG | - | chr8.1:20039100-20039119 | MS.gene059963:intron | 45.0% |
| ! | CTCGGAATCCAAAGCATCAT+TGG | + | chr8.1:20039102-20039121 | None:intergenic | 45.0% |
| ! | CTTCAGATTGAAGGTGTGTC+CGG | - | chr8.1:20039448-20039467 | MS.gene059963:intron | 45.0% |
| ! | CTTCTCCGGGTTTTTGTTAC+AGG | - | chr8.1:20040063-20040082 | MS.gene059963:intron | 45.0% |
| !!! | GCCTTGGAGCTTTTAGAGTT+AGG | - | chr8.1:20039298-20039317 | MS.gene059963:intron | 45.0% |
| AGCCGTACAGATTCGAAGGT+TGG | + | chr8.1:20038836-20038855 | None:intergenic | 50.0% | |
| ATTGCAGAGACTGAGGGTGA+TGG | - | chr8.1:20040213-20040232 | MS.gene059963:intron | 50.0% | |
| CAGCCTCTGCATCAACAGAA+AGG | + | chr8.1:20040176-20040195 | None:intergenic | 50.0% | |
| CCGACACTTCAGATTGGAAG+CGG | - | chr8.1:20039376-20039395 | MS.gene059963:intron | 50.0% | |
| CGACACTTCAGATTGGAAGC+GGG | - | chr8.1:20039377-20039396 | MS.gene059963:intron | 50.0% | |
| GCTTACCTGTGAACTTCTCC+GGG | - | chr8.1:20040050-20040069 | MS.gene059963:intron | 50.0% | |
| GGATAGCCGTACAGATTCGA+AGG | + | chr8.1:20038840-20038859 | None:intergenic | 50.0% | |
| TTACTGCTACAGGTAGCAGC+TGG | - | chr8.1:20039839-20039858 | MS.gene059963:intron | 50.0% | |
| ! | ACTGGTTGGGTGCTATCGAT+AGG | - | chr8.1:20039694-20039713 | MS.gene059963:intron | 50.0% |
| ! | CCGCTTCCAATCTGAAGTGT+CGG | + | chr8.1:20039379-20039398 | None:intergenic | 50.0% |
| ! | CTCGTACATGGTAGTGACAC+AGG | + | chr8.1:20039541-20039560 | None:intergenic | 50.0% |
| !! | AAGCACTGGCATTAGCTGCA+TGG | + | chr8.1:20040026-20040045 | None:intergenic | 50.0% |
| !! | AGCACTGGCATTAGCTGCAT+GGG | + | chr8.1:20040025-20040044 | None:intergenic | 50.0% |
| !! | GATTGGAAGCGGGTTTTGTG+TGG | - | chr8.1:20039387-20039406 | MS.gene059963:intron | 50.0% |
| !! | ATCGGTGTCGTGTCGGATAC+CGG | + | chr8.1:20039470-20039489 | None:intergenic | 55.0% |
| !! | TGTCGCTATCGGTGTCGTGT+CGG | + | chr8.1:20039477-20039496 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 20038824 | 20040293 | 20038824 | ID=MS.gene059963 |
| chr8.1 | mRNA | 20038824 | 20040293 | 20038824 | ID=MS.gene059963.t1;Parent=MS.gene059963 |
| chr8.1 | exon | 20040270 | 20040293 | 20040270 | ID=MS.gene059963.t1.exon1;Parent=MS.gene059963.t1 |
| chr8.1 | CDS | 20040270 | 20040293 | 20040270 | ID=cds.MS.gene059963.t1;Parent=MS.gene059963.t1 |
| chr8.1 | exon | 20040128 | 20040176 | 20040128 | ID=MS.gene059963.t1.exon2;Parent=MS.gene059963.t1 |
| chr8.1 | CDS | 20040128 | 20040176 | 20040128 | ID=cds.MS.gene059963.t1;Parent=MS.gene059963.t1 |
| chr8.1 | exon | 20039996 | 20040036 | 20039996 | ID=MS.gene059963.t1.exon3;Parent=MS.gene059963.t1 |
| chr8.1 | CDS | 20039996 | 20040036 | 20039996 | ID=cds.MS.gene059963.t1;Parent=MS.gene059963.t1 |
| chr8.1 | exon | 20039227 | 20039266 | 20039227 | ID=MS.gene059963.t1.exon4;Parent=MS.gene059963.t1 |
| chr8.1 | CDS | 20039227 | 20039266 | 20039227 | ID=cds.MS.gene059963.t1;Parent=MS.gene059963.t1 |
| chr8.1 | exon | 20039033 | 20039086 | 20039033 | ID=MS.gene059963.t1.exon5;Parent=MS.gene059963.t1 |
| chr8.1 | CDS | 20039033 | 20039086 | 20039033 | ID=cds.MS.gene059963.t1;Parent=MS.gene059963.t1 |
| chr8.1 | exon | 20038824 | 20038927 | 20038824 | ID=MS.gene059963.t1.exon6;Parent=MS.gene059963.t1 |
| chr8.1 | CDS | 20038824 | 20038927 | 20038824 | ID=cds.MS.gene059963.t1;Parent=MS.gene059963.t1 |
| Gene Sequence |
| Protein sequence |
