AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07407


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07407.t1 MTR_1g076310 98.214 112 2 0 1 112 1 112 1.06e-79 230
MS.gene07407.t1 MTR_2g039750 59.048 105 36 1 1 98 1 105 2.36e-39 128
MS.gene07407.t1 MTR_5g014070 58.095 105 37 1 1 98 1 105 3.80e-38 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07407.t1 AT4G34620 65.854 82 28 0 1 82 1 82 4.62e-38 124
MS.gene07407.t1 AT5G56940 53.846 104 41 1 1 97 1 104 5.68e-38 125

Find 25 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACAACAACCCGGTAAAA+TGG 0.190138 1.2:-64162920 None:intergenic
TGGATGGCTGCTCTTCGTTC+GGG 0.277399 1.2:-64164612 None:intergenic
TTGGATGGCTGCTCTTCGTT+CGG 0.292007 1.2:-64164613 None:intergenic
ACCATCAACTTCTTTGTCAT+TGG 0.341893 1.2:-64164632 None:intergenic
GGTTACTACAATCCTATTGC+AGG 0.364917 1.2:+64162986 MS.gene07407:CDS
TCTTCGTTCGGGGTATCAAC+AGG 0.369294 1.2:-64164601 None:intergenic
ATTGATGTTGAACCTGCAAT+AGG 0.387393 1.2:-64162998 None:intergenic
TCCAATGACAAAGAAGTTGA+TGG 0.440759 1.2:+64164631 MS.gene07407:CDS
TTTAGGTATTGGCTTTCTGT+TGG 0.451329 1.2:+64164493 MS.gene07407:intron
TTACAACAACCCGGTAAAAT+GGG 0.462639 1.2:-64162919 None:intergenic
AAACGGAAAGGTGGACTGTC+TGG 0.470726 1.2:+64164571 MS.gene07407:CDS
TCAACTTCTTTGTCATTGGA+TGG 0.482393 1.2:-64164628 None:intergenic
AGGCAGGTTTACAAATGAAA+CGG 0.502953 1.2:+64164554 MS.gene07407:CDS
AGTATTCGATTAGCGAGACT+TGG 0.516156 1.2:+64162884 MS.gene07407:CDS
GGCAAGAACATCGAAGTTGT+TGG 0.525645 1.2:+64162965 MS.gene07407:CDS
AGACTCTCCACAGGTTCTGA+AGG 0.549004 1.2:-64164523 None:intergenic
TGCTCAACCTTCAGAACCTG+TGG 0.580737 1.2:+64164516 MS.gene07407:CDS
TTGAATAAAAGACTCTCCAC+AGG 0.595461 1.2:-64164532 None:intergenic
GGATGGCTGCTCTTCGTTCG+GGG 0.597237 1.2:-64164611 None:intergenic
CTTCGTTCGGGGTATCAACA+GGG 0.597733 1.2:-64164600 None:intergenic
AGATTCACATTCAAAAGTGA+AGG 0.606410 1.2:-64164666 None:intergenic
GATAGCAAATGTGCAAGAGA+CGG 0.614709 1.2:+64162944 MS.gene07407:CDS
TGCTATCAGTTACAACAACC+CGG 0.633843 1.2:-64162928 None:intergenic
TTACAAATGAAACGGAAAGG+TGG 0.647965 1.2:+64164562 MS.gene07407:CDS
GGTTTACAAATGAAACGGAA+AGG 0.664042 1.2:+64164559 MS.gene07407:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTTTTTTAAAATTA+CGG + chr1.2:64163784-64163803 MS.gene07407:intron 0.0%
!! AAAATTTCAGAAAATTATTA+TGG - chr1.2:64163692-64163711 None:intergenic 10.0%
!!! AAAATTGCATTTTTATTATT+TGG + chr1.2:64163082-64163101 MS.gene07407:intron 10.0%
!!! TATATGCAATATTTTATTTT+TGG - chr1.2:64163537-64163556 None:intergenic 10.0%
!! AAAGTAAATCAAGTTAAAAA+AGG - chr1.2:64164310-64164329 None:intergenic 15.0%
!!! AAAGAATTTTTCTTTCATTT+GGG + chr1.2:64163113-64163132 MS.gene07407:intron 15.0%
!! AACATATAGAATTAGAGATA+AGG - chr1.2:64163031-64163050 None:intergenic 20.0%
!! AATCAAGTTAAAAAAGGATT+AGG - chr1.2:64164304-64164323 None:intergenic 20.0%
!! CAAATGAAAGAAAAATTCTT+TGG - chr1.2:64163114-64163133 None:intergenic 20.0%
!! TAATTGATATTTGCATATGT+AGG + chr1.2:64163370-64163389 MS.gene07407:intron 20.0%
!! TATGAATATTGCATATAGAA+GGG + chr1.2:64163505-64163524 MS.gene07407:intron 20.0%
!! TGTTTATAAATGATAAAACC+AGG - chr1.2:64164111-64164130 None:intergenic 20.0%
!! TTATGAATATTGCATATAGA+AGG + chr1.2:64163504-64163523 MS.gene07407:intron 20.0%
!!! AGTTATTTGTACACAATTTT+GGG + chr1.2:64164415-64164434 MS.gene07407:intron 20.0%
!!! CAAAGAATTTTTCTTTCATT+TGG + chr1.2:64163112-64163131 MS.gene07407:intron 20.0%
!!! CATTTTTAATCCTTTTACTA+TGG - chr1.2:64164191-64164210 None:intergenic 20.0%
!!! GGTTTTATCATTTATAAACA+CGG + chr1.2:64164111-64164130 MS.gene07407:intron 20.0%
!!! TAGTTATTTGTACACAATTT+TGG + chr1.2:64164414-64164433 MS.gene07407:intron 20.0%
!!! TTAAAGCACAAAATTGTTTT+GGG + chr1.2:64163159-64163178 MS.gene07407:intron 20.0%
! AACGAACTGATGTATTTAAA+CGG + chr1.2:64163975-64163994 MS.gene07407:intron 25.0%
! AAGAGAGAGATAAAGATAAA+CGG + chr1.2:64163261-64163280 MS.gene07407:intron 25.0%
! AGCATACAATAATGTTCTAA+AGG - chr1.2:64163644-64163663 None:intergenic 25.0%
! ATGATTTACAATTACCTACA+TGG + chr1.2:64163818-64163837 MS.gene07407:intron 25.0%
! ATGTTCAAATAACATAACTC+GGG + chr1.2:64163303-64163322 MS.gene07407:intron 25.0%
! GAAAATTATTATGGCAAGTA+AGG - chr1.2:64163683-64163702 None:intergenic 25.0%
! GATGTTCAAATAACATAACT+CGG + chr1.2:64163302-64163321 MS.gene07407:intron 25.0%
! TTATCATTTATAAACACGGA+GGG + chr1.2:64164115-64164134 MS.gene07407:intron 25.0%
! TTGTATGCTTAATTTCACTT+TGG + chr1.2:64163655-64163674 MS.gene07407:intron 25.0%
! TTTATCATTTATAAACACGG+AGG + chr1.2:64164114-64164133 MS.gene07407:intron 25.0%
! TTTCATTTGGGTATACTTTA+TGG + chr1.2:64163125-64163144 MS.gene07407:intron 25.0%
!! ATCCTTTTACTATGGAATTA+TGG - chr1.2:64164183-64164202 None:intergenic 25.0%
!! CTTAAAGCACAAAATTGTTT+TGG + chr1.2:64163158-64163177 MS.gene07407:intron 25.0%
!! GTTATTTGTACACAATTTTG+GGG + chr1.2:64164416-64164435 MS.gene07407:intron 25.0%
!! TTCTACTTTGTAAAGTATCA+TGG + chr1.2:64164028-64164047 MS.gene07407:intron 25.0%
AAATGACCATAATGAAACAC+AGG + chr1.2:64163581-64163600 MS.gene07407:intron 30.0%
AATTGCAATGTATACCAGTT+TGG + chr1.2:64164245-64164264 MS.gene07407:intron 30.0%
ATTAAATGGCAGATTGACAT+AGG - chr1.2:64163771-64163790 None:intergenic 30.0%
ATTGCAATGTATACCAGTTT+GGG + chr1.2:64164246-64164265 MS.gene07407:intron 30.0%
CTTATCTGTTTGCATCTTAA+TGG + chr1.2:64163901-64163920 MS.gene07407:intron 30.0%
GAGATAAAGATAAACGGATA+TGG + chr1.2:64163267-64163286 MS.gene07407:intron 30.0%
GCAATTCACAGTAACAAATA+GGG - chr1.2:64164231-64164250 None:intergenic 30.0%
GGAAATTAGTGATAATTGTG+GGG + chr1.2:64164437-64164456 MS.gene07407:intron 30.0%
GGGAAATTAGTGATAATTGT+GGG + chr1.2:64164436-64164455 MS.gene07407:intron 30.0%
TAATCAATAACCCAAACTGT+TGG + chr1.2:64164157-64164176 MS.gene07407:intron 30.0%
TCAACTTGAAATGAAAACAC+CGG - chr1.2:64163733-64163752 None:intergenic 30.0%
TGCAATTCACAGTAACAAAT+AGG - chr1.2:64164232-64164251 None:intergenic 30.0%
TTACAATTACCTACATGGTA+TGG + chr1.2:64163823-64163842 MS.gene07407:intron 30.0%
! ATATGTAGGACTTGTTTGAA+AGG + chr1.2:64163384-64163403 MS.gene07407:intron 30.0%
! CATTTTTGCTAATGAGTAGT+GGG + chr1.2:64163346-64163365 MS.gene07407:intron 30.0%
! GAATTTTCTGTGTTTACGAT+AGG + chr1.2:64163845-64163864 MS.gene07407:intron 30.0%
!! GTTGTGAGTTTCTAGATTTT+GGG + chr1.2:64163420-64163439 MS.gene07407:intron 30.0%
!! TGTTGTGAGTTTCTAGATTT+TGG + chr1.2:64163419-64163438 MS.gene07407:intron 30.0%
!!! TTTTTTGTTCCTAGCTCTAA+GGG - chr1.2:64163479-64163498 None:intergenic 30.0%
AAACACCGGAAAAACTTACA+CGG - chr1.2:64163719-64163738 None:intergenic 35.0%
ACCATAATGAAACACAGGTT+TGG + chr1.2:64163586-64163605 MS.gene07407:intron 35.0%
ACCATCAACTTCTTTGTCAT+TGG - chr1.2:64164635-64164654 None:intergenic 35.0%
AGGCAGGTTTACAAATGAAA+CGG + chr1.2:64164554-64164573 MS.gene07407:CDS 35.0%
ATTGATGTTGAACCTGCAAT+AGG - chr1.2:64163001-64163020 None:intergenic 35.0%
CAATACCTAAAGAACATGAG+GGG - chr1.2:64164484-64164503 None:intergenic 35.0%
CAGAAAATTCCATACCATGT+AGG - chr1.2:64163835-64163854 None:intergenic 35.0%
CATTTGGGTATACTTTATGG+TGG + chr1.2:64163128-64163147 MS.gene07407:intron 35.0%
CCAATACCTAAAGAACATGA+GGG - chr1.2:64164485-64164504 None:intergenic 35.0%
CTGATTTCTGTTCTCAGTAT+GGG + chr1.2:64163877-64163896 MS.gene07407:intron 35.0%
GGCCATAATTCCATAGTAAA+AGG + chr1.2:64164178-64164197 MS.gene07407:intron 35.0%
GGGGAAATTAGTGATAATTG+TGG + chr1.2:64164435-64164454 MS.gene07407:intron 35.0%
GGTTTACAAATGAAACGGAA+AGG + chr1.2:64164559-64164578 MS.gene07407:CDS 35.0%
TCAACTTCTTTGTCATTGGA+TGG - chr1.2:64164631-64164650 None:intergenic 35.0%
TCCAAACCTGTGTTTCATTA+TGG - chr1.2:64163590-64163609 None:intergenic 35.0%
TCCAATGACAAAGAAGTTGA+TGG + chr1.2:64164631-64164650 MS.gene07407:CDS 35.0%
TTACAAATGAAACGGAAAGG+TGG + chr1.2:64164562-64164581 MS.gene07407:CDS 35.0%
TTACAACAACCCGGTAAAAT+GGG - chr1.2:64162922-64162941 None:intergenic 35.0%
TTGAATAAAAGACTCTCCAC+AGG - chr1.2:64164535-64164554 None:intergenic 35.0%
! GCATTTTTGCTAATGAGTAG+TGG + chr1.2:64163345-64163364 MS.gene07407:intron 35.0%
! GGGATAAACACGTTTTCTTA+TGG + chr1.2:64163323-64163342 MS.gene07407:intron 35.0%
! TGTGGAGAGTCTTTTATTCA+AGG + chr1.2:64164534-64164553 MS.gene07407:CDS 35.0%
! TTGTTCCGTGTAAGTTTTTC+CGG + chr1.2:64163711-64163730 MS.gene07407:intron 35.0%
!! TTTAGGTATTGGCTTTCTGT+TGG + chr1.2:64164493-64164512 MS.gene07407:intron 35.0%
!!! GTTTTTTGTTCCTAGCTCTA+AGG - chr1.2:64163480-64163499 None:intergenic 35.0%
AGTATTCGATTAGCGAGACT+TGG + chr1.2:64162884-64162903 MS.gene07407:CDS 40.0%
CCATACTGAGAACAGAAATC+AGG - chr1.2:64163879-64163898 None:intergenic 40.0%
CCCTCATGTTCTTTAGGTAT+TGG + chr1.2:64164482-64164501 MS.gene07407:intron 40.0%
CCTGATTTCTGTTCTCAGTA+TGG + chr1.2:64163876-64163895 MS.gene07407:intron 40.0%
CGATCGTTATCTCTTGTTAG+TGG + chr1.2:64163614-64163633 MS.gene07407:intron 40.0%
CTAGAAAGTGACCTGATAGA+GGG + chr1.2:64164084-64164103 MS.gene07407:intron 40.0%
GATAGCAAATGTGCAAGAGA+CGG + chr1.2:64162944-64162963 MS.gene07407:CDS 40.0%
GCCAATACCTAAAGAACATG+AGG - chr1.2:64164486-64164505 None:intergenic 40.0%
GGAATTATGGCCAACAGTTT+GGG - chr1.2:64164170-64164189 None:intergenic 40.0%
GGCAGATTGACATAGGTTAT+AGG - chr1.2:64163764-64163783 None:intergenic 40.0%
GGTTACTACAATCCTATTGC+AGG + chr1.2:64162986-64163005 MS.gene07407:CDS 40.0%
GTGATAATTGTGGGGATCAT+TGG + chr1.2:64164445-64164464 MS.gene07407:intron 40.0%
GTTACAACAACCCGGTAAAA+TGG - chr1.2:64162923-64162942 None:intergenic 40.0%
TAGAAAGTGACCTGATAGAG+GGG + chr1.2:64164085-64164104 MS.gene07407:intron 40.0%
TGCTATCAGTTACAACAACC+CGG - chr1.2:64162931-64162950 None:intergenic 40.0%
TGGAATTATGGCCAACAGTT+TGG - chr1.2:64164171-64164190 None:intergenic 40.0%
TTTAGCTATCCCTTAGAGCT+AGG + chr1.2:64163467-64163486 MS.gene07407:intron 40.0%
! GAGAGTCTTTTATTCAAGGC+AGG + chr1.2:64164538-64164557 MS.gene07407:CDS 40.0%
! TATACCAGTTTGGGATGAGA+AGG + chr1.2:64164255-64164274 MS.gene07407:intron 40.0%
!! AAACGGATTGAAGTCACTTG+AGG + chr1.2:64163992-64164011 MS.gene07407:intron 40.0%
AACATGAGGGGAGGTTAAGA+TGG - chr1.2:64164472-64164491 None:intergenic 45.0%
AACCTCCCCTCATGTTCTTT+AGG + chr1.2:64164476-64164495 MS.gene07407:intron 45.0%
GCTAGAAAGTGACCTGATAG+AGG + chr1.2:64164083-64164102 MS.gene07407:intron 45.0%
GGCAAGAACATCGAAGTTGT+TGG + chr1.2:64162965-64162984 MS.gene07407:CDS 45.0%
TACCTAAAGAACATGAGGGG+AGG - chr1.2:64164481-64164500 None:intergenic 45.0%
! CACACACAACCCATTTTACC+GGG + chr1.2:64162910-64162929 MS.gene07407:CDS 45.0%
! GCACACACAACCCATTTTAC+CGG + chr1.2:64162909-64162928 MS.gene07407:CDS 45.0%
!!! GTAATTTTAAAAAAATTAAA+TGG - chr1.2:64163785-64163804 None:intergenic 5.0%
TAAAACCAGGCCCCTCTATC+AGG - chr1.2:64164098-64164117 None:intergenic 50.0%
TGCTCAACCTTCAGAACCTG+TGG + chr1.2:64164516-64164535 MS.gene07407:CDS 50.0%
TTGGATGGCTGCTCTTCGTT+CGG - chr1.2:64164616-64164635 None:intergenic 50.0%
! ACCAGTTTGGGATGAGAAGG+AGG + chr1.2:64164258-64164277 MS.gene07407:intron 50.0%
! AGACTCTCCACAGGTTCTGA+AGG - chr1.2:64164526-64164545 None:intergenic 50.0%
! CCAGTTTGGGATGAGAAGGA+GGG + chr1.2:64164259-64164278 MS.gene07407:intron 50.0%
! CTTCGTTCGGGGTATCAACA+GGG - chr1.2:64164603-64164622 None:intergenic 50.0%
! TCTTCGTTCGGGGTATCAAC+AGG - chr1.2:64164604-64164623 None:intergenic 50.0%
!! AAACGGAAAGGTGGACTGTC+TGG + chr1.2:64164571-64164590 MS.gene07407:CDS 50.0%
CCCTCCTTCTCATCCCAAAC+TGG - chr1.2:64164262-64164281 None:intergenic 55.0%
TGGATGGCTGCTCTTCGTTC+GGG - chr1.2:64164615-64164634 None:intergenic 55.0%
AGTGACCTGATAGAGGGGCC+TGG + chr1.2:64164090-64164109 MS.gene07407:intron 60.0%
GGATGGCTGCTCTTCGTTCG+GGG - chr1.2:64164614-64164633 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 64162875 64164678 64162875 ID=MS.gene07407
chr1.2 mRNA 64162875 64164678 64162875 ID=MS.gene07407.t1;Parent=MS.gene07407
chr1.2 exon 64162875 64163007 64162875 ID=MS.gene07407.t1.exon1;Parent=MS.gene07407.t1
chr1.2 CDS 64162875 64163007 64162875 ID=cds.MS.gene07407.t1;Parent=MS.gene07407.t1
chr1.2 exon 64164337 64164382 64164337 ID=MS.gene07407.t1.exon2;Parent=MS.gene07407.t1
chr1.2 CDS 64164337 64164382 64164337 ID=cds.MS.gene07407.t1;Parent=MS.gene07407.t1
chr1.2 exon 64164498 64164678 64164498 ID=MS.gene07407.t1.exon3;Parent=MS.gene07407.t1
chr1.2 CDS 64164498 64164678 64164498 ID=cds.MS.gene07407.t1;Parent=MS.gene07407.t1
Gene Sequence

>MS.gene07407

ATGGTGGTGAGTATTCGATTAGCGAGACTTGGATGCACACACAACCCATTTTACCGGGTTGTTGTAACTGATAGCAAATGTGCAAGAGACGGCAAGAACATCGAAGTTGTTGGTTACTACAATCCTATTGCAGGTTCAACATCAATTCTTTAACCTTATCTCTAATTCTATATGTTTTTATGCTCAAATTTTACTTTTGTGTTATTAAAAATTGCATTTTTATTATTTGGTGAAAGCCAAAGAATTTTTCTTTCATTTGGGTATACTTTATGGTGGATTAGTACTTAAAGCACAAAATTGTTTTGGGATAGAGAAGTGATATTTTGACATTAAAATTTTGATGTCAAATATAGTGTACTTTGTTTTGATGTTGCAGAAGATAAAACAAGAGAGAGATAAAGATAAACGGATATGGTGTCTGTATTTTGATGTTCAAATAACATAACTCGGGATAAACACGTTTTCTTATGGCATTTTTGCTAATGAGTAGTGGGTTAATTGATATTTGCATATGTAGGACTTGTTTGAAAGGATATAGAATTAGTGTTGTGAGTTTCTAGATTTTGGGATGTAATGAAATAGTAAGAATCACTTTAGCTATCCCTTAGAGCTAGGAACAAAAAACACAGTTATGAATATTGCATATAGAAGGGTCAACACCAAAAATAAAATATTGCATATAGAAACTATCTTGAATTTTTCGCTCAAATGACCATAATGAAACACAGGTTTGGATCATCGATCGTTATCTCTTGTTAGTGGCGTTCCTTTAGAACATTATTGTATGCTTAATTTCACTTTGGTTCCTTACTTGCCATAATAATTTTCTGAAATTTTTGTTCCGTGTAAGTTTTTCCGGTGTTTTCATTTCAAGTTGAAACACGTACCTATAACCTATGTCAATCTGCCATTTAATTTTTTTAAAATTACGGTGATGTGACTAATGATTTACAATTACCTACATGGTATGGAATTTTCTGTGTTTACGATAGGTTCATTAACCTGATTTCTGTTCTCAGTATGGGACTTATCTGTTTGCATCTTAATGGACTTAGTAGTGACTTTTATCTATATATTAAGCGAGTGACATATTTAATTATAACGAACTGATGTATTTAAACGGATTGAAGTCACTTGAGGTTTCATTCAGATGTTCTACTTTGTAAAGTATCATGGCGCATAAATTGTTATCTGCATCACTATTTTTTGCTAGAAAGTGACCTGATAGAGGGGCCTGGTTTTATCATTTATAAACACGGAGGGATTTGATTGTTTAAAAAATTAATCAATAACCCAAACTGTTGGCCATAATTCCATAGTAAAAGGATTAAAAATGCAAGTTGCAAATGTCATCCCTATTTGTTACTGTGAATTGCAATGTATACCAGTTTGGGATGAGAAGGAGGGTGATTCAAGTTGATACGCACCTAATCCTTTTTTAACTTGATTTACTTTTGTACAGCTAAAGATGATGAGAAAAGAGTGAACATCAAACTTGAAAGAGTGAAGTAAGTAACTTTTATTTAATTCTAATTTATCTAGTTATTTGTACACAATTTTGGGGAAATTAGTGATAATTGTGGGGATCATTGGTCCATCTTAACCTCCCCTCATGTTCTTTAGGTATTGGCTTTCTGTTGGTGCTCAACCTTCAGAACCTGTGGAGAGTCTTTTATTCAAGGCAGGTTTACAAATGAAACGGAAAGGTGGACTGTCTGGTAGCTACCCTGTTGATACCCCGAACGAAGAGCAGCCATCCAATGACAAAGAAGTTGATGGTAAACTGACACTCCTTCACTTTTGA

Protein sequence

>MS.gene07407.t1

MVVSIRLARLGCTHNPFYRVVVTDSKCARDGKNIEVVGYYNPIAAKDDEKRVNIKLERVKYWLSVGAQPSEPVESLLFKAGLQMKRKGGLSGSYPVDTPNEEQPSNDKEVDGKLTLLHF