AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060181

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060181.t1 MTR_2g076690 95.624 457 20 0 1 457 1 457 0.0 913
MS.gene060181.t1 MTR_2g076840 83.778 450 70 3 7 456 1 447 0.0 787
MS.gene060181.t1 MTR_2g076840 65.632 419 93 6 44 461 361 729 6.38e-173 503
MS.gene060181.t1 MTR_2g076920 79.583 480 72 4 1 461 1 473 0.0 782
MS.gene060181.t1 MTR_2g076710 84.375 448 61 3 3 443 1 446 0.0 776
MS.gene060181.t1 MTR_2g076970 81.153 451 84 1 7 457 1 450 0.0 772
MS.gene060181.t1 MTR_2g076900 79.610 461 86 3 7 461 1 459 0.0 762
MS.gene060181.t1 MTR_2g076990 82.785 395 66 1 65 457 1 395 0.0 668
MS.gene060181.t1 MTR_8g076920 45.572 463 236 5 6 455 15 474 1.61e-149 436
MS.gene060181.t1 MTR_5g020980 45.648 471 243 6 1 462 7 473 5.11e-148 432
MS.gene060181.t1 MTR_5g020990 42.444 450 233 7 21 447 36 482 1.82e-120 360
MS.gene060181.t1 MTR_4g107370 40.496 484 235 8 16 455 15 489 2.51e-115 348
MS.gene060181.t1 MTR_1g022250 43.137 459 220 11 16 445 12 458 8.30e-113 340
MS.gene060181.t1 MTR_2g030420 40.351 456 246 5 16 451 15 464 3.29e-112 339
MS.gene060181.t1 MTR_7g008040 40.741 459 225 9 16 435 10 460 8.76e-107 328
MS.gene060181.t1 MTR_6g009720 38.961 462 232 9 16 434 14 468 6.36e-106 325
MS.gene060181.t1 MTR_6g009553 39.825 457 212 11 30 435 4 448 2.72e-103 317
MS.gene060181.t1 MTR_6g009520 40.260 462 220 11 16 435 14 461 3.49e-103 317
MS.gene060181.t1 MTR_6g009770 40.460 435 204 9 46 435 23 447 3.52e-103 317
MS.gene060181.t1 MTR_6g042290 39.427 454 238 11 5 435 12 451 3.90e-102 314
MS.gene060181.t1 MTR_4g128260 37.344 482 250 10 16 460 10 476 4.49e-102 315
MS.gene060181.t1 MTR_6g009540 38.855 489 246 11 16 459 12 492 6.29e-99 306
MS.gene060181.t1 MTR_1g022245 37.660 470 242 9 7 436 1 459 1.43e-98 305
MS.gene060181.t1 MTR_2g061820 36.100 482 252 10 14 457 3 466 1.18e-97 303
MS.gene060181.t1 MTR_3g103150 50.538 279 130 4 162 435 1 276 1.74e-91 279
MS.gene060181.t1 MTR_6g009510 37.020 443 219 9 5 435 12 406 3.64e-88 276
MS.gene060181.t1 MTR_6g009660 37.947 419 217 13 36 435 17 411 5.68e-78 251
MS.gene060181.t1 MTR_3g092060 33.421 380 214 9 68 437 66 416 1.49e-68 227
MS.gene060181.t1 MTR_5g021020 34.954 432 230 10 10 435 19 405 2.47e-68 224
MS.gene060181.t1 MTR_7g117950 33.053 357 200 8 65 415 61 384 1.54e-64 216
MS.gene060181.t1 MTR_8g027200 30.025 393 212 12 54 435 48 388 4.51e-47 169
MS.gene060181.t1 MTR_2g076820 92.424 66 5 0 366 431 1 66 1.70e-36 129
MS.gene060181.t1 MTR_5g021000 62.857 105 37 1 333 435 1 105 1.73e-36 132
MS.gene060181.t1 MTR_5g020720 40.437 183 76 4 264 435 25 185 8.04e-32 120
MS.gene060181.t1 MTR_1g011680 43.089 123 70 0 318 440 14 136 1.08e-28 112
MS.gene060181.t1 MTR_2g076910 68.000 75 23 1 7 81 1 74 2.10e-28 107
MS.gene060181.t1 MTR_5g021010 50.476 105 44 3 339 435 17 121 1.73e-21 90.1
MS.gene060181.t1 MTR_2g076830 76.596 47 11 0 404 450 9 55 7.01e-17 75.5
MS.gene060181.t1 MTR_3g117230 30.047 213 131 7 190 393 129 332 4.72e-15 77.0
MS.gene060181.t1 MTR_3g112260 26.855 283 185 9 190 459 126 399 1.24e-14 75.9
MS.gene060181.t1 MTR_3g062510 26.804 194 127 6 216 401 171 357 1.27e-14 75.9
MS.gene060181.t1 MTR_3g062510 28.313 166 106 4 216 373 171 331 1.84e-14 75.1
MS.gene060181.t1 MTR_7g099640 30.270 185 109 8 210 383 194 369 1.79e-13 72.4
MS.gene060181.t1 MTR_7g099640 30.270 185 109 8 210 383 194 369 2.00e-13 72.4
MS.gene060181.t1 MTR_1g083330 30.488 164 99 5 230 384 193 350 4.05e-13 71.2
MS.gene060181.t1 MTR_4g033235 30.488 164 99 5 230 384 194 351 4.65e-13 71.2
MS.gene060181.t1 MTR_7g089040 32.026 153 88 6 228 372 408 552 8.86e-13 71.2
MS.gene060181.t1 MTR_4g094270 29.609 179 105 7 210 376 163 332 1.63e-12 69.3
MS.gene060181.t1 MTR_7g096060 29.319 191 122 5 206 388 242 427 2.46e-12 69.7
MS.gene060181.t1 MTR_5g069780 31.214 173 106 5 204 368 313 480 5.82e-12 68.6
MS.gene060181.t1 MTR_7g010800 28.495 186 117 6 201 377 203 381 1.71e-11 67.0
MS.gene060181.t1 MTR_3g462930 30.857 175 108 5 204 370 304 473 1.78e-11 67.0
MS.gene060181.t1 MTR_8g099605 30.814 172 98 6 221 376 179 345 2.23e-11 66.6
MS.gene060181.t1 MTR_8g099605 30.994 171 97 6 222 376 421 586 3.06e-11 66.2
MS.gene060181.t1 MTR_1g113750 41.053 95 50 3 366 454 16 110 4.43e-11 60.8
MS.gene060181.t1 MTR_7g101870 25.490 255 133 10 161 373 136 375 4.82e-11 65.5
MS.gene060181.t1 MTR_7g101870 27.684 177 115 5 205 373 480 651 7.14e-11 65.1
MS.gene060181.t1 MTR_1g063380 28.571 168 107 5 213 372 487 649 5.36e-11 65.5
MS.gene060181.t1 MTR_3g023890 26.267 217 138 6 211 417 105 309 5.64e-11 65.1
MS.gene060181.t1 MTR_2g095210 28.571 168 107 5 213 372 415 577 8.50e-11 64.7
MS.gene060181.t1 MTR_7g089290 24.176 273 169 9 128 388 579 825 9.94e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060181.t1 AT3G50940 52.758 417 196 1 25 440 30 446 3.44e-159 458
MS.gene060181.t1 AT3G50930 48.430 446 211 5 23 452 74 516 3.94e-149 437
MS.gene060181.t1 AT2G18193 43.624 447 222 5 16 447 20 451 1.02e-128 382
MS.gene060181.t1 AT5G17740 40.959 459 258 4 16 462 19 476 2.12e-123 370
MS.gene060181.t1 AT2G18190 42.366 465 239 6 4 450 5 458 1.87e-122 366
MS.gene060181.t1 AT5G17760 41.597 476 254 7 10 463 9 482 6.88e-122 365
MS.gene060181.t1 AT5G17730 41.189 454 244 10 10 447 9 455 8.46e-120 358
MS.gene060181.t1 AT3G28600 41.342 462 217 9 16 441 9 452 1.03e-114 346
MS.gene060181.t1 AT3G28510 40.263 457 231 8 16 438 13 461 1.23e-111 340
MS.gene060181.t1 AT3G28540 39.956 458 218 8 16 432 13 454 7.34e-110 334
MS.gene060181.t1 AT3G28540 39.956 458 218 8 16 432 13 454 1.11e-109 334
MS.gene060181.t1 AT4G05380 41.226 473 236 11 3 447 1 459 2.62e-109 331
MS.gene060181.t1 AT3G28610 40.336 476 223 13 3 441 1 452 9.13e-109 330
MS.gene060181.t1 AT3G28580 39.659 469 231 11 1 431 1 455 8.35e-108 329
MS.gene060181.t1 AT4G25835 40.323 434 223 8 16 420 10 436 1.95e-106 325
MS.gene060181.t1 AT5G40010 40.088 454 228 9 16 431 13 460 1.83e-104 321
MS.gene060181.t1 AT5G40000 39.167 480 239 10 7 451 4 465 5.48e-102 313
MS.gene060181.t1 AT5G57480 35.870 460 251 6 16 437 10 463 1.37e-101 313
MS.gene060181.t1 AT5G17750 40.342 409 205 9 5 403 11 390 1.45e-101 309
MS.gene060181.t1 AT3G28570 38.333 480 243 14 2 456 3 454 4.59e-100 307
MS.gene060181.t1 AT3G28570 39.140 465 231 13 16 456 11 447 5.09e-100 307
MS.gene060181.t1 AT1G43910 38.004 471 245 8 4 443 1 455 1.33e-99 307
MS.gene060181.t1 AT3G28600 39.341 455 197 8 16 441 9 413 1.64e-99 305
MS.gene060181.t1 AT3G28520 39.278 471 244 8 18 455 1 462 6.06e-97 300
MS.gene060181.t1 AT3G28580 39.111 450 238 11 1 431 1 433 4.20e-96 298
MS.gene060181.t1 AT4G30250 36.229 472 249 12 7 437 1 461 2.55e-95 297
MS.gene060181.t1 AT4G30250 36.229 472 249 12 7 437 3 463 2.66e-95 297
MS.gene060181.t1 AT2G46620 33.333 426 230 10 54 455 49 444 3.44e-74 241
MS.gene060181.t1 AT5G17760 39.623 318 175 4 10 312 9 324 4.22e-72 231
MS.gene060181.t1 AT3G29800 31.448 442 232 13 30 449 11 403 6.18e-64 213
MS.gene060181.t1 AT4G05340 60.294 68 27 0 323 390 26 93 3.05e-20 85.9
MS.gene060181.t1 AT3G28560 29.534 193 106 5 35 214 36 211 2.51e-18 85.1
MS.gene060181.t1 AT1G45000 28.521 284 179 11 190 459 126 399 4.05e-15 77.4
MS.gene060181.t1 AT5G58290 26.214 206 137 6 204 401 152 350 4.34e-15 77.4
MS.gene060181.t1 AT5G43010 30.256 195 119 6 190 376 126 311 4.65e-15 77.4
MS.gene060181.t1 AT3G05530 31.098 164 98 5 230 384 194 351 1.84e-13 72.4
MS.gene060181.t1 AT4G29040 30.337 178 105 7 217 383 198 367 3.02e-13 72.0
MS.gene060181.t1 AT2G20140 29.714 175 110 5 217 383 198 367 6.06e-13 70.9
MS.gene060181.t1 AT5G19990 29.050 179 106 7 210 376 107 276 1.13e-12 69.7
MS.gene060181.t1 AT5G20000 29.050 179 106 7 210 376 165 334 1.93e-12 69.3
MS.gene060181.t1 AT5G19990 29.609 179 105 7 210 376 165 334 2.00e-12 69.3
MS.gene060181.t1 AT5G19990 29.609 179 105 7 210 376 165 334 2.00e-12 69.3
MS.gene060181.t1 AT5G58870 30.857 175 108 5 204 370 326 495 6.08e-12 68.6
MS.gene060181.t1 AT2G03670 31.013 158 85 6 234 376 49 197 1.04e-11 67.4
MS.gene060181.t1 AT2G03670 31.013 158 85 6 234 376 49 197 1.34e-11 66.6
MS.gene060181.t1 AT3G47060 30.508 177 110 5 204 372 322 493 2.64e-11 66.6
MS.gene060181.t1 AT3G53230 28.402 169 108 5 213 373 487 650 9.92e-11 64.7

Find 70 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGATTGAAGTTCTTCT+TGG 0.223470 2.3:+20479859 MS.gene060181:CDS
CTTCTCTCTTAGTCTCTTCC+TGG 0.235607 2.3:-20479990 None:intergenic
TTTCAACAGATGACACTTTC+GGG 0.253746 2.3:+20479631 MS.gene060181:intron
ATTGGATGGTTGAACTTAAT+AGG 0.269533 2.3:-20478824 None:intergenic
TTGAAATTCTTCAATGACTA+TGG 0.286598 2.3:-20478402 None:intergenic
AAGGGAAATTAAGAATGATT+TGG 0.292518 2.3:+20478883 MS.gene060181:CDS
TTGTATGGTCCTCCTGGTAC+TGG 0.303664 2.3:+20478971 MS.gene060181:CDS
GATGAAGAAGTTGTTCATAA+TGG 0.310288 2.3:+20479172 MS.gene060181:CDS
CAACTTCTTCATCTTCTTCT+CGG 0.328790 2.3:-20479162 None:intergenic
TGAATGCAGTAGATGGTCTT+TGG 0.365588 2.3:+20479659 MS.gene060181:CDS
TGTTCATAATGGGCACAATA+AGG 0.386190 2.3:+20479183 MS.gene060181:CDS
AGTTAAAGCAACCAAATCTA+AGG 0.392234 2.3:+20478499 MS.gene060181:CDS
ATGAAGAAGTTGTTCATAAT+GGG 0.392993 2.3:+20479173 MS.gene060181:CDS
ACCGCGTTTCCAAGCTTTCC+CGG 0.395418 2.3:-20478942 None:intergenic
GTCCCGATTGAACAAAGTTT+AGG 0.399697 2.3:-20478343 None:intergenic
AGTTGCTTTAGTTCCTAGAT+AGG 0.400041 2.3:-20478462 None:intergenic
GGAATTTGATAAGGTCTTGC+AGG 0.402644 2.3:-20479948 None:intergenic
ACTTGGATCTCACTAATGTT+GGG 0.408726 2.3:+20479047 MS.gene060181:CDS
AACTCTTCTGCAATTTCAGC+TGG 0.411104 2.3:-20479898 None:intergenic
GAGGCAGCAGAAGCCTATCT+AGG 0.421960 2.3:+20478449 MS.gene060181:CDS
AGTCCTAAACTTTGTTCAAT+CGG 0.423323 2.3:+20478340 MS.gene060181:CDS
TGGCTTGGAGGAATTTGATA+AGG 0.429033 2.3:-20479957 None:intergenic
ATTCCTCCAAGCCAAGAAAA+TGG 0.431539 2.3:+20479966 MS.gene060181:CDS
GAGGCCACTGCAGATAAAAC+TGG 0.436495 2.3:-20478272 None:intergenic
TGGTGAATTGTGCGTTGAAT+TGG 0.444709 2.3:-20478382 None:intergenic
CCGTTCTATACTTGTGATAG+AGG 0.447088 2.3:+20479111 MS.gene060181:CDS
GTCCTAAACTTTGTTCAATC+GGG 0.449195 2.3:+20478341 MS.gene060181:CDS
GACTTGGATCTCACTAATGT+TGG 0.453484 2.3:+20479046 MS.gene060181:CDS
TTGAAGGTGTTAGTGTAAAG+TGG 0.455356 2.3:+20478576 MS.gene060181:CDS
TACTTGTTGTATGGTCCTCC+TGG 0.457478 2.3:+20478965 MS.gene060181:CDS
GCAAGAGTGTTGAAACTCAT+TGG 0.462379 2.3:-20478842 None:intergenic
TTAGCTCTCTCCATCACATA+AGG 0.464927 2.3:-20478734 None:intergenic
TCACAACAAATCACAAAGAC+AGG 0.471357 2.3:+20479713 MS.gene060181:CDS
AAACGCGGTTACTTGTTGTA+TGG 0.474020 2.3:+20478956 MS.gene060181:CDS
ATAGAAGAACCGGGAAAGCT+TGG 0.478091 2.3:+20478933 MS.gene060181:CDS
AGCAAAGAAGAACAAGATCT+AGG 0.484846 2.3:+20480018 MS.gene060181:CDS
CAATTGATGAAGAGCTTCAA+AGG 0.486235 2.3:+20478864 MS.gene060181:CDS
ATAGTCATTGAAGAATTTCA+AGG 0.496242 2.3:+20478404 MS.gene060181:CDS
GTCTAGCTTGATTGCAGCCA+TGG 0.496694 2.3:+20478997 MS.gene060181:CDS
AAGCTAGACTTCCCAGTACC+AGG 0.497146 2.3:-20478983 None:intergenic
GGACTATTGAATGCAGTAGA+TGG 0.497808 2.3:+20479652 MS.gene060181:CDS
CCAAGCCAAGAAAATGGTCC+AGG 0.508568 2.3:+20479972 MS.gene060181:CDS
GATCTAGGCAGAGAAAATAT+AGG 0.509105 2.3:+20480033 MS.gene060181:CDS
CAACTATGATATCTATGACT+TGG 0.513130 2.3:+20479030 MS.gene060181:CDS
ATTTGCTTGTCCATTCTACC+AGG 0.513687 2.3:-20479757 None:intergenic
AGTTCGCATTAGCGCAATTG+AGG 0.515687 2.3:-20478291 None:intergenic
TTCATATTTGCCTTATGTGA+TGG 0.523892 2.3:+20478724 MS.gene060181:CDS
TGTATGGTCCTCCTGGTACT+GGG 0.528478 2.3:+20478972 MS.gene060181:CDS
AATTGATGAAGAGCTTCAAA+GGG 0.533769 2.3:+20478865 MS.gene060181:CDS
TCTGTTAAGACCTGGTAGAA+TGG 0.545715 2.3:+20479747 MS.gene060181:CDS
CTACCAGGTCTTAACAGAGC+AGG 0.546075 2.3:-20479742 None:intergenic
TATCACAAGTATAGAACGGT+TGG 0.555140 2.3:-20479107 None:intergenic
ACAAATTAAACAAGGAATTG+TGG 0.559399 2.3:+20478757 MS.gene060181:CDS
ACTCAAATGAATATAATCGT+TGG 0.570247 2.3:+20478792 MS.gene060181:CDS
ACATTATGATAGGGACTCCT+CGG 0.578511 2.3:+20478637 MS.gene060181:CDS
CTTGGATCTCACTAATGTTG+GGG 0.595649 2.3:+20479048 MS.gene060181:CDS
GATCCTGCTCTGTTAAGACC+TGG 0.601081 2.3:+20479739 MS.gene060181:CDS
GTTCATGTTCAGTAATGCAA+AGG 0.603050 2.3:-20479831 None:intergenic
TAGTCATTGAAGAATTTCAA+GGG 0.606257 2.3:+20478405 MS.gene060181:CDS
GAGTGTTGAAACTCATTGGA+TGG 0.615289 2.3:-20478838 None:intergenic
ACCGGGAAAGCTTGGAAACG+CGG 0.621197 2.3:+20478941 MS.gene060181:CDS
TCTTATTTCTGACACAGCCG+AGG 0.630279 2.3:-20478654 None:intergenic
CCTCTATCACAAGTATAGAA+CGG 0.641804 2.3:-20479111 None:intergenic
ATAGTTGAGATAGTTAGCCA+TGG 0.661661 2.3:-20479014 None:intergenic
GATGGTCTTTGGTCATGCTG+TGG 0.668698 2.3:+20479670 MS.gene060181:CDS
ACAAAGTTTAGGACTTCACG+AGG 0.668909 2.3:-20478332 None:intergenic
AGAGCTAAACAAATTAAACA+AGG 0.691321 2.3:+20478749 MS.gene060181:CDS
AATTGCAGAAGAGTTAACAA+AGG 0.702150 2.3:+20479906 MS.gene060181:CDS
TCTTTGGTCATGCTGTGGAG+AGG 0.703389 2.3:+20479675 MS.gene060181:CDS
CTAGACTTCCCAGTACCAGG+AGG 0.750100 2.3:-20478980 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTAGCTGAAAAAATT+TGG + chr2.3:20479384-20479403 MS.gene060181:intron 15.0%
!! AAAAATTCGACATTATGATA+GGG + chr2.3:20478628-20478647 MS.gene060181:CDS 20.0%
!! TCTTTCAAATAATTACAGTT+TGG - chr2.3:20479257-20479276 None:intergenic 20.0%
!!! AAAGAATTTTATAGAAGAAC+CGG + chr2.3:20478923-20478942 MS.gene060181:CDS 20.0%
!!! ATTAGTTTAAAATAGTTGAC+AGG - chr2.3:20479597-20479616 None:intergenic 20.0%
! AAGGGAAATTAAGAATGATT+TGG + chr2.3:20478883-20478902 MS.gene060181:CDS 25.0%
! ACAAATTAAACAAGGAATTG+TGG + chr2.3:20478757-20478776 MS.gene060181:CDS 25.0%
! ACTCAAATGAATATAATCGT+TGG + chr2.3:20478792-20478811 MS.gene060181:CDS 25.0%
! AGAGCTAAACAAATTAAACA+AGG + chr2.3:20478749-20478768 MS.gene060181:CDS 25.0%
! ATAGTCATTGAAGAATTTCA+AGG + chr2.3:20478404-20478423 MS.gene060181:CDS 25.0%
! ATGAAGAAGTTGTTCATAAT+GGG + chr2.3:20479173-20479192 MS.gene060181:CDS 25.0%
! CAAAAATTCGACATTATGAT+AGG + chr2.3:20478627-20478646 MS.gene060181:CDS 25.0%
! TAAGTCATGTTGTTAACAAT+AGG - chr2.3:20479348-20479367 None:intergenic 25.0%
! TAGTCATTGAAGAATTTCAA+GGG + chr2.3:20478405-20478424 MS.gene060181:CDS 25.0%
! TTGAAATTCTTCAATGACTA+TGG - chr2.3:20478405-20478424 None:intergenic 25.0%
!! AAGAATTTTATAGAAGAACC+GGG + chr2.3:20478924-20478943 MS.gene060181:CDS 25.0%
!! CTTTTTATGTTCCTTAGATT+TGG - chr2.3:20478513-20478532 None:intergenic 25.0%
!!! CTTTTCTGCTTTTAAGAAAT+TGG + chr2.3:20479798-20479817 MS.gene060181:CDS 25.0%
AATTGATGAAGAGCTTCAAA+GGG + chr2.3:20478865-20478884 MS.gene060181:CDS 30.0%
AATTGCAGAAGAGTTAACAA+AGG + chr2.3:20479906-20479925 MS.gene060181:CDS 30.0%
ACAGGAACAAAAGAATATGT+TGG - chr2.3:20479579-20479598 None:intergenic 30.0%
AGTCCTAAACTTTGTTCAAT+CGG + chr2.3:20478340-20478359 MS.gene060181:CDS 30.0%
AGTTAAAGCAACCAAATCTA+AGG + chr2.3:20478499-20478518 MS.gene060181:CDS 30.0%
ATGATGTCTGTTCATATCAT+GGG + chr2.3:20479535-20479554 MS.gene060181:intron 30.0%
ATTGGATGGTTGAACTTAAT+AGG - chr2.3:20478827-20478846 None:intergenic 30.0%
CAACTATGATATCTATGACT+TGG + chr2.3:20479030-20479049 MS.gene060181:CDS 30.0%
GAAAAGATTGAAGTTCTTCT+TGG + chr2.3:20479859-20479878 MS.gene060181:CDS 30.0%
GATGAAGAAGTTGTTCATAA+TGG + chr2.3:20479172-20479191 MS.gene060181:CDS 30.0%
TATGATGTCTGTTCATATCA+TGG + chr2.3:20479534-20479553 MS.gene060181:intron 30.0%
TTCATATTTGCCTTATGTGA+TGG + chr2.3:20478724-20478743 MS.gene060181:CDS 30.0%
! TTCTCGGTTTTGCAATTTTA+CGG - chr2.3:20479149-20479168 None:intergenic 30.0%
! TTTTCAACAGATGACACTTT+CGG + chr2.3:20479630-20479649 MS.gene060181:intron 30.0%
!! CTGAAAAAATTTGGTCTTGT+TGG + chr2.3:20479393-20479412 MS.gene060181:intron 30.0%
AATCATCTGCATGATTTAGC+CGG + chr2.3:20479311-20479330 MS.gene060181:intron 35.0%
ACTTGGATCTCACTAATGTT+GGG + chr2.3:20479047-20479066 MS.gene060181:CDS 35.0%
AGCAAAGAAGAACAAGATCT+AGG + chr2.3:20480018-20480037 MS.gene060181:CDS 35.0%
ATAGTTGAGATAGTTAGCCA+TGG - chr2.3:20479017-20479036 None:intergenic 35.0%
CAACTTCTTCATCTTCTTCT+CGG - chr2.3:20479165-20479184 None:intergenic 35.0%
CAATTGATGAAGAGCTTCAA+AGG + chr2.3:20478864-20478883 MS.gene060181:CDS 35.0%
CCTCTATCACAAGTATAGAA+CGG - chr2.3:20479114-20479133 None:intergenic 35.0%
CGTTGAATTGGCAAAAAAGA+TGG - chr2.3:20478373-20478392 None:intergenic 35.0%
CTAAATATTATGTCCTCGTG+CGG - chr2.3:20479373-20479392 None:intergenic 35.0%
GAATTTGACGTACATGAATC+TGG + chr2.3:20479275-20479294 MS.gene060181:intron 35.0%
GATCTAGGCAGAGAAAATAT+AGG + chr2.3:20480033-20480052 MS.gene060181:CDS 35.0%
GTCCTAAACTTTGTTCAATC+GGG + chr2.3:20478341-20478360 MS.gene060181:CDS 35.0%
GTTCATGTTCAGTAATGCAA+AGG - chr2.3:20479834-20479853 None:intergenic 35.0%
TATCACAAGTATAGAACGGT+TGG - chr2.3:20479110-20479129 None:intergenic 35.0%
TCACAACAAATCACAAAGAC+AGG + chr2.3:20479713-20479732 MS.gene060181:CDS 35.0%
TGATGTCTGTTCATATCATG+GGG + chr2.3:20479536-20479555 MS.gene060181:intron 35.0%
TTTCAACAGATGACACTTTC+GGG + chr2.3:20479631-20479650 MS.gene060181:intron 35.0%
! AAGATTTTGTCGACAATGGT+TGG + chr2.3:20479417-20479436 MS.gene060181:intron 35.0%
! AGTTGCTTTAGTTCCTAGAT+AGG - chr2.3:20478465-20478484 None:intergenic 35.0%
! GACAAGAAACCAAGTTTTTG+AGG + chr2.3:20478430-20478449 MS.gene060181:CDS 35.0%
! TGGTAAGATTTTGTCGACAA+TGG + chr2.3:20479413-20479432 MS.gene060181:intron 35.0%
!! GAAGTGAGTGATGTTTTTGA+AGG + chr2.3:20478560-20478579 MS.gene060181:CDS 35.0%
!! TGTTCATAATGGGCACAATA+AGG + chr2.3:20479183-20479202 MS.gene060181:CDS 35.0%
!! TTGAAGGTGTTAGTGTAAAG+TGG + chr2.3:20478576-20478595 MS.gene060181:CDS 35.0%
AACTCTTCTGCAATTTCAGC+TGG - chr2.3:20479901-20479920 None:intergenic 40.0%
ACAATAGGACCAAAATTCGC+CGG - chr2.3:20479333-20479352 None:intergenic 40.0%
ACATTATGATAGGGACTCCT+CGG + chr2.3:20478637-20478656 MS.gene060181:CDS 40.0%
ATTCCTCCAAGCCAAGAAAA+TGG + chr2.3:20479966-20479985 MS.gene060181:CDS 40.0%
ATTTGCTTGTCCATTCTACC+AGG - chr2.3:20479760-20479779 None:intergenic 40.0%
CAAACTATGTACATACGACG+AGG + chr2.3:20479469-20479488 MS.gene060181:intron 40.0%
CCGTTCTATACTTGTGATAG+AGG + chr2.3:20479111-20479130 MS.gene060181:CDS 40.0%
CTTGGATCTCACTAATGTTG+GGG + chr2.3:20479048-20479067 MS.gene060181:CDS 40.0%
GACTTGGATCTCACTAATGT+TGG + chr2.3:20479046-20479065 MS.gene060181:CDS 40.0%
GGAATTTGATAAGGTCTTGC+AGG - chr2.3:20479951-20479970 None:intergenic 40.0%
GGACTATTGAATGCAGTAGA+TGG + chr2.3:20479652-20479671 MS.gene060181:CDS 40.0%
GTCCCGATTGAACAAAGTTT+AGG - chr2.3:20478346-20478365 None:intergenic 40.0%
TCTGTTAAGACCTGGTAGAA+TGG + chr2.3:20479747-20479766 MS.gene060181:CDS 40.0%
TGAATGCAGTAGATGGTCTT+TGG + chr2.3:20479659-20479678 MS.gene060181:CDS 40.0%
TGGTGAATTGTGCGTTGAAT+TGG - chr2.3:20478385-20478404 None:intergenic 40.0%
! ACAAAGTTTAGGACTTCACG+AGG - chr2.3:20478335-20478354 None:intergenic 40.0%
! ATGATTTAGCCGGCGAATTT+TGG + chr2.3:20479321-20479340 MS.gene060181:intron 40.0%
! TGGCTTGGAGGAATTTGATA+AGG - chr2.3:20479960-20479979 None:intergenic 40.0%
! TTAGCTCTCTCCATCACATA+AGG - chr2.3:20478737-20478756 None:intergenic 40.0%
!! AAACGCGGTTACTTGTTGTA+TGG + chr2.3:20478956-20478975 MS.gene060181:CDS 40.0%
!! GAGTGTTGAAACTCATTGGA+TGG - chr2.3:20478841-20478860 None:intergenic 40.0%
!! GCAAGAGTGTTGAAACTCAT+TGG - chr2.3:20478845-20478864 None:intergenic 40.0%
AACATGACTTATACCGCACG+AGG + chr2.3:20479357-20479376 MS.gene060181:intron 45.0%
AGTTCGCATTAGCGCAATTG+AGG - chr2.3:20478294-20478313 None:intergenic 45.0%
ATAGAAGAACCGGGAAAGCT+TGG + chr2.3:20478933-20478952 MS.gene060181:CDS 45.0%
CTTCTCTCTTAGTCTCTTCC+TGG - chr2.3:20479993-20480012 None:intergenic 45.0%
CTTCTGCTGCCTCAAAAACT+TGG - chr2.3:20478442-20478461 None:intergenic 45.0%
TGTACATACGACGAGGTCAT+AGG + chr2.3:20479476-20479495 MS.gene060181:intron 45.0%
! CTCTTCCTGGACCATTTTCT+TGG - chr2.3:20479980-20479999 None:intergenic 45.0%
! TCGACAATGGTTGGTCAAGT+TGG + chr2.3:20479426-20479445 MS.gene060181:intron 45.0%
!! CAAGCCAGTTTTATCTGCAG+TGG + chr2.3:20478268-20478287 MS.gene060181:CDS 45.0%
!! TACTTGTTGTATGGTCCTCC+TGG + chr2.3:20478965-20478984 MS.gene060181:CDS 45.0%
!! TCTTATTTCTGACACAGCCG+AGG - chr2.3:20478657-20478676 None:intergenic 45.0%
AAGCTAGACTTCCCAGTACC+AGG - chr2.3:20478986-20479005 None:intergenic 50.0%
CCAAGCCAAGAAAATGGTCC+AGG + chr2.3:20479972-20479991 MS.gene060181:CDS 50.0%
CTACCAGGTCTTAACAGAGC+AGG - chr2.3:20479745-20479764 None:intergenic 50.0%
GAGGCCACTGCAGATAAAAC+TGG - chr2.3:20478275-20478294 None:intergenic 50.0%
GATCCTGCTCTGTTAAGACC+TGG + chr2.3:20479739-20479758 MS.gene060181:CDS 50.0%
GATGGTCTTTGGTCATGCTG+TGG + chr2.3:20479670-20479689 MS.gene060181:CDS 50.0%
TCTTTGGTCATGCTGTGGAG+AGG + chr2.3:20479675-20479694 MS.gene060181:CDS 50.0%
TGTATGGTCCTCCTGGTACT+GGG + chr2.3:20478972-20478991 MS.gene060181:CDS 50.0%
TTGTATGGTCCTCCTGGTAC+TGG + chr2.3:20478971-20478990 MS.gene060181:CDS 50.0%
! CCTGGACCATTTTCTTGGCT+TGG - chr2.3:20479975-20479994 None:intergenic 50.0%
! GTCTAGCTTGATTGCAGCCA+TGG + chr2.3:20478997-20479016 MS.gene060181:CDS 50.0%
!!! GGACCATTTTCTTGGCTTGG+AGG - chr2.3:20479972-20479991 None:intergenic 50.0%
ACCGCGTTTCCAAGCTTTCC+CGG - chr2.3:20478945-20478964 None:intergenic 55.0%
ACCGGGAAAGCTTGGAAACG+CGG + chr2.3:20478941-20478960 MS.gene060181:CDS 55.0%
CTAGACTTCCCAGTACCAGG+AGG - chr2.3:20478983-20479002 None:intergenic 55.0%
GAGGCAGCAGAAGCCTATCT+AGG + chr2.3:20478449-20478468 MS.gene060181:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 20478236 20480068 20478236 ID=MS.gene060181
chr2.3 mRNA 20478236 20480068 20478236 ID=MS.gene060181.t1;Parent=MS.gene060181
chr2.3 exon 20478236 20479204 20478236 ID=MS.gene060181.t1.exon1;Parent=MS.gene060181.t1
chr2.3 CDS 20478236 20479204 20478236 ID=cds.MS.gene060181.t1;Parent=MS.gene060181.t1
chr2.3 exon 20479640 20480068 20479640 ID=MS.gene060181.t1.exon2;Parent=MS.gene060181.t1
chr2.3 CDS 20479640 20480068 20479640 ID=cds.MS.gene060181.t1;Parent=MS.gene060181.t1
Gene Sequence

>MS.gene060181

ATGACAATGATCTCAACAATGTTTGATAGCAGCAAGCCAGTTTTATCTGCAGTGGCCTCAATTGCGCTAATGCGAACTGTCACAAATGAACTCATTCCTCGTGAAGTCCTAAACTTTGTTCAATCGGGACTTCACCATCTTTTTTGCCAATTCAACGCACAATTCACCATAGTCATTGAAGAATTTCAAGGGATGACAAGAAACCAAGTTTTTGAGGCAGCAGAAGCCTATCTAGGAACTAAAGCAACTGTCTCAGCAGAAAGAGTTAAAGCAACCAAATCTAAGGAACATAAAAAGCTTTCTTTCAATCTAGATAGAGATGAAGAAGTGAGTGATGTTTTTGAAGGTGTTAGTGTAAAGTGGAAACTAATATGCATACAAGTCGACTCATCAAAAATTCGACATTATGATAGGGACTCCTCGGCTGTGTCAGAAATAAGATCATATGAGCTAACTTTTCACAAGAAACACAAAAACAAGATCTTTGATTCATATTTGCCTTATGTGATGGAGAGAGCTAAACAAATTAAACAAGGAATTGTGGCACTTAAGATTCACTCAAATGAATATAATCGTTGGTGTCATGATCCTATTAAGTTCAACCATCCAATGAGTTTCAACACTCTTGCAATTGATGAAGAGCTTCAAAGGGAAATTAAGAATGATTTGGACAAATTTGTGAGTGCTAAAGAATTTTATAGAAGAACCGGGAAAGCTTGGAAACGCGGTTACTTGTTGTATGGTCCTCCTGGTACTGGGAAGTCTAGCTTGATTGCAGCCATGGCTAACTATCTCAACTATGATATCTATGACTTGGATCTCACTAATGTTGGGGATAATAAGAGCTTAAAGCAACTTATTCTCAGCATGTCCAACCGTTCTATACTTGTGATAGAGGATATTGATTGCTCCGTAAAATTGCAAAACCGAGAAGAAGATGAAGAAGTTGTTCATAATGGGCACAATAAGGTATGAATTTCTCTTTGAAAATATGATTCATCTTTGAGTTTCTTTCATACCAAACTGTAATTATTTGAAAGAATTTGACGTACATGAATCTGGTTTAGATCTATTTAATCATCTGCATGATTTAGCCGGCGAATTTTGGTCCTATTGTTAACAACATGACTTATACCGCACGAGGACATAATATTTAGCTGAAAAAATTTGGTCTTGTTGGTAAGATTTTGTCGACAATGGTTGGTCAAGTTGGTAAAATATGACATGTATTTTCAAACTATGTACATACGACGAGGTCATAGGTTCAAAATACTTACATATGTAAGTACGCTTTGATTTATGATGTCTGTTCATATCATGGGGACTAATTGAAAGCAATACCAACATATTCTTTTGTTCCTGTCAACTATTTTAAACTAATGTTTGTTGATACTTTTTCAACAGATGACACTTTCGGGACTATTGAATGCAGTAGATGGTCTTTGGTCATGCTGTGGAGAGGAACACATAATTGTTTTCACAACAAATCACAAAGACAGGCTTGATCCTGCTCTGTTAAGACCTGGTAGAATGGACAAGCAAATTCACTTGTCATATTGCAACTTTTCTGCTTTTAAGAAATTGGTTATCAACTACCTTTGCATTACTGAACATGAACTCTTTGAAAAGATTGAAGTTCTTCTTGGACAAGTTCAAGTTACTCCAGCTGAAATTGCAGAAGAGTTAACAAAGGATGTTGATGCTACAGAATGCCTGCAAGACCTTATCAAATTCCTCCAAGCCAAGAAAATGGTCCAGGAAGAGACTAAGAGAGAAGAAAATAGCAAAGAAGAACAAGATCTAGGCAGAGAAAATATAGGTCTATCTAAGTAG
Protein sequence

>MS.gene060181.t1

MTMISTMFDSSKPVLSAVASIALMRTVTNELIPREVLNFVQSGLHHLFCQFNAQFTIVIEEFQGMTRNQVFEAAEAYLGTKATVSAERVKATKSKEHKKLSFNLDRDEEVSDVFEGVSVKWKLICIQVDSSKIRHYDRDSSAVSEIRSYELTFHKKHKNKIFDSYLPYVMERAKQIKQGIVALKIHSNEYNRWCHDPIKFNHPMSFNTLAIDEELQREIKNDLDKFVSAKEFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLNYDIYDLDLTNVGDNKSLKQLILSMSNRSILVIEDIDCSVKLQNREEDEEVVHNGHNKMTLSGLLNAVDGLWSCCGEEHIIVFTTNHKDRLDPALLRPGRMDKQIHLSYCNFSAFKKLVINYLCITEHELFEKIEVLLGQVQVTPAEIAEELTKDVDATECLQDLIKFLQAKKMVQEETKREENSKEEQDLGRENIGLSK