AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63758.t1 MTR_2g076690 95.186 457 22 0 1 457 1 457 0.0 909
MS.gene63758.t1 MTR_2g076840 83.556 450 71 3 7 456 1 447 0.0 785
MS.gene63758.t1 MTR_2g076840 65.394 419 94 6 44 461 361 729 7.35e-173 503
MS.gene63758.t1 MTR_2g076920 79.167 480 74 4 1 461 1 473 0.0 778
MS.gene63758.t1 MTR_2g076710 83.929 448 63 3 3 443 1 446 0.0 773
MS.gene63758.t1 MTR_2g076970 80.931 451 85 1 7 457 1 450 0.0 769
MS.gene63758.t1 MTR_2g076900 79.826 461 85 3 7 461 1 459 0.0 764
MS.gene63758.t1 MTR_2g076990 82.785 395 66 1 65 457 1 395 0.0 669
MS.gene63758.t1 MTR_8g076920 45.969 459 232 5 10 455 19 474 2.82e-150 437
MS.gene63758.t1 MTR_5g020980 46.053 456 236 5 16 462 19 473 1.53e-146 428
MS.gene63758.t1 MTR_5g020990 42.000 450 235 7 21 447 36 482 4.74e-119 357
MS.gene63758.t1 MTR_4g107370 40.404 495 240 10 6 455 5 489 2.85e-114 346
MS.gene63758.t1 MTR_1g022250 42.612 467 231 10 6 445 2 458 9.35e-113 340
MS.gene63758.t1 MTR_2g030420 40.132 456 247 5 16 451 15 464 3.70e-112 339
MS.gene63758.t1 MTR_7g008040 40.523 459 226 9 16 435 10 460 1.44e-106 327
MS.gene63758.t1 MTR_6g009720 38.528 462 234 9 16 434 14 468 1.46e-104 321
MS.gene63758.t1 MTR_6g009520 40.043 462 221 11 16 435 14 461 6.23e-103 317
MS.gene63758.t1 MTR_6g009770 39.910 446 211 10 37 435 12 447 7.48e-103 316
MS.gene63758.t1 MTR_6g009553 39.387 457 214 11 30 435 4 448 4.07e-102 313
MS.gene63758.t1 MTR_4g128260 36.590 481 255 8 16 460 10 476 2.11e-101 313
MS.gene63758.t1 MTR_6g042290 38.987 454 240 11 5 435 12 451 6.07e-101 311
MS.gene63758.t1 MTR_6g009540 39.059 489 245 12 16 459 12 492 1.69e-98 305
MS.gene63758.t1 MTR_1g022245 37.447 470 243 9 7 436 1 459 1.93e-98 305
MS.gene63758.t1 MTR_2g061820 35.892 482 253 10 14 457 3 466 6.08e-98 304
MS.gene63758.t1 MTR_3g103150 50.179 279 131 4 162 435 1 276 3.70e-91 278
MS.gene63758.t1 MTR_6g009510 36.795 443 220 9 5 435 12 406 4.57e-87 274
MS.gene63758.t1 MTR_6g009660 37.864 412 214 12 43 435 23 411 3.29e-77 249
MS.gene63758.t1 MTR_3g092060 33.684 380 213 10 68 437 66 416 2.81e-68 226
MS.gene63758.t1 MTR_5g021020 34.491 432 232 10 10 435 19 405 4.24e-67 221
MS.gene63758.t1 MTR_7g117950 32.773 357 201 8 65 415 61 384 2.23e-64 215
MS.gene63758.t1 MTR_8g027200 29.771 393 213 12 54 435 48 388 7.23e-47 168
MS.gene63758.t1 MTR_2g076820 90.909 66 6 0 366 431 1 66 2.85e-36 129
MS.gene63758.t1 MTR_5g021000 61.905 105 38 1 333 435 1 105 3.39e-36 131
MS.gene63758.t1 MTR_5g020720 39.891 183 77 4 264 435 25 185 1.68e-31 120
MS.gene63758.t1 MTR_1g011680 42.276 123 71 0 318 440 14 136 2.12e-28 110
MS.gene63758.t1 MTR_2g076910 66.667 75 24 1 7 81 1 74 1.82e-27 105
MS.gene63758.t1 MTR_5g021010 49.524 105 45 3 339 435 17 121 3.19e-21 89.4
MS.gene63758.t1 MTR_2g076830 76.596 47 11 0 404 450 9 55 6.94e-17 75.5
MS.gene63758.t1 MTR_3g117230 30.256 195 119 6 190 376 129 314 6.42e-15 76.6
MS.gene63758.t1 MTR_3g062510 25.773 194 129 5 216 401 171 357 1.15e-14 76.3
MS.gene63758.t1 MTR_3g112260 29.744 195 120 6 190 376 126 311 1.62e-14 75.5
MS.gene63758.t1 MTR_3g062510 28.313 166 106 4 216 373 171 331 2.09e-14 75.1
MS.gene63758.t1 MTR_7g099640 30.270 185 109 8 210 383 194 369 2.03e-13 72.4
MS.gene63758.t1 MTR_7g099640 30.270 185 109 8 210 383 194 369 2.20e-13 72.4
MS.gene63758.t1 MTR_1g083330 30.488 164 99 5 230 384 193 350 5.25e-13 70.9
MS.gene63758.t1 MTR_4g033235 30.488 164 99 5 230 384 194 351 5.71e-13 70.9
MS.gene63758.t1 MTR_7g089040 32.026 153 88 6 228 372 408 552 9.50e-13 71.2
MS.gene63758.t1 MTR_4g094270 29.609 179 105 7 210 376 163 332 1.57e-12 69.3
MS.gene63758.t1 MTR_7g096060 29.319 191 122 5 206 388 242 427 3.17e-12 69.3
MS.gene63758.t1 MTR_5g069780 31.214 173 106 5 204 368 313 480 6.24e-12 68.6
MS.gene63758.t1 MTR_7g010800 28.495 186 117 6 201 377 203 381 1.56e-11 67.0
MS.gene63758.t1 MTR_3g462930 30.857 175 108 5 204 370 304 473 2.05e-11 66.6
MS.gene63758.t1 MTR_8g099605 30.814 172 98 6 221 376 179 345 2.31e-11 66.6
MS.gene63758.t1 MTR_8g099605 30.994 171 97 6 222 376 421 586 2.95e-11 66.2
MS.gene63758.t1 MTR_1g063380 28.571 168 107 5 213 372 487 649 4.82e-11 65.5
MS.gene63758.t1 MTR_7g101870 25.490 255 133 10 161 373 136 375 5.40e-11 65.5
MS.gene63758.t1 MTR_7g101870 27.684 177 115 5 205 373 480 651 7.33e-11 65.1
MS.gene63758.t1 MTR_3g023890 26.267 217 138 6 211 417 105 309 7.21e-11 65.1
MS.gene63758.t1 MTR_2g095210 28.571 168 107 5 213 372 415 577 7.79e-11 64.7
MS.gene63758.t1 MTR_1g113750 40.000 95 51 3 366 454 16 110 9.42e-11 59.7
MS.gene63758.t1 MTR_7g089290 27.950 161 103 4 236 388 670 825 9.51e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63758.t1 AT3G50940 52.518 417 197 1 25 440 30 446 3.43e-158 456
MS.gene63758.t1 AT3G50930 48.206 446 212 5 23 452 74 516 2.39e-148 435
MS.gene63758.t1 AT2G18193 43.722 446 223 5 16 447 20 451 1.73e-127 379
MS.gene63758.t1 AT5G17740 40.523 459 260 4 16 462 19 476 4.14e-122 367
MS.gene63758.t1 AT2G18190 42.151 465 240 6 4 450 5 458 1.89e-121 363
MS.gene63758.t1 AT5G17760 41.286 482 259 7 4 463 3 482 5.26e-121 363
MS.gene63758.t1 AT5G17730 40.969 454 245 10 10 447 9 455 9.66e-119 356
MS.gene63758.t1 AT3G28600 40.909 462 219 9 16 441 9 452 1.95e-113 342
MS.gene63758.t1 AT3G28510 40.044 457 232 8 16 438 13 461 1.47e-110 337
MS.gene63758.t1 AT3G28540 39.738 458 219 8 16 432 13 454 5.28e-109 332
MS.gene63758.t1 AT3G28540 39.738 458 219 8 16 432 13 454 6.95e-109 332
MS.gene63758.t1 AT4G05380 40.803 473 238 11 3 447 1 459 3.47e-108 328
MS.gene63758.t1 AT3G28610 40.126 476 224 13 3 441 1 452 5.85e-108 328
MS.gene63758.t1 AT3G28580 39.570 465 237 11 1 431 1 455 1.50e-107 328
MS.gene63758.t1 AT4G25835 39.261 433 229 6 16 420 10 436 1.02e-105 323
MS.gene63758.t1 AT5G40010 39.868 454 229 9 16 431 13 460 5.49e-104 319
MS.gene63758.t1 AT5G57480 35.652 460 252 6 16 437 10 463 2.61e-101 313
MS.gene63758.t1 AT5G40000 38.958 480 240 11 7 451 4 465 9.12e-101 310
MS.gene63758.t1 AT5G17750 39.759 415 210 9 3 403 2 390 9.51e-101 307
MS.gene63758.t1 AT3G28570 37.787 479 247 12 2 456 3 454 9.97e-100 306
MS.gene63758.t1 AT3G28570 38.578 464 235 11 16 456 11 447 1.29e-99 306
MS.gene63758.t1 AT1G43910 38.065 465 242 7 10 443 6 455 8.28e-99 305
MS.gene63758.t1 AT3G28600 38.901 455 199 8 16 441 9 413 3.59e-98 302
MS.gene63758.t1 AT3G28520 39.066 471 245 8 18 455 1 462 5.10e-97 300
MS.gene63758.t1 AT3G28580 38.565 446 246 10 1 431 1 433 5.86e-96 298
MS.gene63758.t1 AT4G30250 35.957 470 255 9 6 437 2 463 1.31e-95 298
MS.gene63758.t1 AT4G30250 35.821 469 255 9 7 437 1 461 5.11e-95 296
MS.gene63758.t1 AT2G46620 33.099 426 231 10 54 455 49 444 5.54e-74 241
MS.gene63758.t1 AT5G17760 39.506 324 179 4 4 312 3 324 1.23e-71 230
MS.gene63758.t1 AT3G29800 31.222 442 233 13 30 449 11 403 1.13e-63 213
MS.gene63758.t1 AT4G05340 58.824 68 28 0 323 390 26 93 5.79e-20 85.1
MS.gene63758.t1 AT3G28560 29.534 193 106 5 35 214 36 211 7.31e-18 83.6
MS.gene63758.t1 AT5G58290 25.243 206 139 5 204 401 152 350 3.75e-15 77.4
MS.gene63758.t1 AT5G43010 30.256 195 119 6 190 376 126 311 4.24e-15 77.4
MS.gene63758.t1 AT1G45000 30.256 195 119 6 190 376 126 311 5.72e-15 77.0
MS.gene63758.t1 AT3G05530 31.098 164 98 5 230 384 194 351 1.92e-13 72.4
MS.gene63758.t1 AT4G29040 30.337 178 105 7 217 383 198 367 3.88e-13 71.6
MS.gene63758.t1 AT2G20140 29.714 175 110 5 217 383 198 367 7.58e-13 70.9
MS.gene63758.t1 AT5G19990 29.050 179 106 7 210 376 107 276 1.02e-12 69.7
MS.gene63758.t1 AT5G19990 29.609 179 105 7 210 376 165 334 1.78e-12 69.3
MS.gene63758.t1 AT5G19990 29.609 179 105 7 210 376 165 334 1.78e-12 69.3
MS.gene63758.t1 AT5G20000 29.050 179 106 7 210 376 165 334 1.93e-12 69.3
MS.gene63758.t1 AT5G58870 30.857 175 108 5 204 370 326 495 7.00e-12 68.2
MS.gene63758.t1 AT2G03670 31.013 158 85 6 234 376 49 197 1.23e-11 67.4
MS.gene63758.t1 AT2G03670 31.013 158 85 6 234 376 49 197 1.51e-11 66.6
MS.gene63758.t1 AT3G47060 30.508 177 110 5 204 372 322 493 2.88e-11 66.2
MS.gene63758.t1 AT3G53230 28.402 169 108 5 213 373 487 650 9.17e-11 64.7

Find 72 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGATTGAAGTTCTTCT+TGG 0.223470 2.1:+23264278 MS.gene63758:CDS
CTTCTCTCTTAGTCTCTTCC+TGG 0.235607 2.1:-23264409 None:intergenic
TTTCAACAGATGACACTTTC+GGG 0.253746 2.1:+23264050 MS.gene63758:intron
ATTGGATGGTTGAACTTAAT+AGG 0.269533 2.1:-23263243 None:intergenic
TTGAAATTCTTCAATGACTA+TGG 0.286598 2.1:-23262821 None:intergenic
AAGGGAAATTAAGAATGATT+TGG 0.292518 2.1:+23263302 MS.gene63758:CDS
TTGTATGGTCCTCCTGGTAC+TGG 0.303664 2.1:+23263390 MS.gene63758:CDS
GATGAAGAAGTTGTTCATAA+TGG 0.310288 2.1:+23263591 MS.gene63758:CDS
CAACTTCTTCATCTTCTTCT+CGG 0.328790 2.1:-23263581 None:intergenic
CAAGAATTCGACATTATGAT+AGG 0.351643 2.1:+23263046 MS.gene63758:CDS
TGAATGCAGTAGATGGTCTT+TGG 0.365588 2.1:+23264078 MS.gene63758:CDS
TGTTCATAATGGGCACAATA+AGG 0.386190 2.1:+23263602 MS.gene63758:CDS
AGTTAAAGCAACCAAATCTA+AGG 0.392234 2.1:+23262918 MS.gene63758:CDS
ATGAAGAAGTTGTTCATAAT+GGG 0.392993 2.1:+23263592 MS.gene63758:CDS
ACCGCGTTTCCAAGCTTTCC+CGG 0.395418 2.1:-23263361 None:intergenic
AGTTGCTTTAGTTCCTAGAT+AGG 0.400041 2.1:-23262881 None:intergenic
GGAATTTGATAAGGTCTTGC+AGG 0.402644 2.1:-23264367 None:intergenic
GTCCCGATTGAACAATGTTT+AGG 0.406316 2.1:-23262762 None:intergenic
ACTTGGATCTCACTAATGTT+GGG 0.408726 2.1:+23263466 MS.gene63758:CDS
AACTCTTCTGCAATTTCAGC+TGG 0.411104 2.1:-23264317 None:intergenic
GAGGCAGCAGAAGCCTATCT+AGG 0.421960 2.1:+23262868 MS.gene63758:CDS
TGGCTTGGAGGAATTTGATA+AGG 0.429033 2.1:-23264376 None:intergenic
ATTCCTCCAAGCCAAGAAAA+TGG 0.431539 2.1:+23264385 MS.gene63758:CDS
GAGGCCACTGCAGATAAAAC+TGG 0.436495 2.1:-23262691 None:intergenic
AGTCCTAAACATTGTTCAAT+CGG 0.445916 2.1:+23262759 MS.gene63758:CDS
CCGTTCTATACTTGTGATAG+AGG 0.447088 2.1:+23263530 MS.gene63758:CDS
GACTTGGATCTCACTAATGT+TGG 0.453484 2.1:+23263465 MS.gene63758:CDS
TTGAAGGTGTTAGTGTAAAG+TGG 0.455356 2.1:+23262995 MS.gene63758:CDS
TGGTGAATTGCGCGTTGAAT+TGG 0.456678 2.1:-23262801 None:intergenic
TACTTGTTGTATGGTCCTCC+TGG 0.457478 2.1:+23263384 MS.gene63758:CDS
GCAAGAGTGTTGAAACTCAT+TGG 0.462379 2.1:-23263261 None:intergenic
TTAGCTCTCTCCATCACATA+AGG 0.464927 2.1:-23263153 None:intergenic
TCACAACAAATCACAAAGAC+AGG 0.471357 2.1:+23264132 MS.gene63758:CDS
AAACGCGGTTACTTGTTGTA+TGG 0.474020 2.1:+23263375 MS.gene63758:CDS
ATAGAAGAACCGGGAAAGCT+TGG 0.478091 2.1:+23263352 MS.gene63758:CDS
AGCAAAGAAGAACAAGATCT+AGG 0.484846 2.1:+23264437 MS.gene63758:CDS
CAATTGATGAAGAGCTTCAA+AGG 0.486235 2.1:+23263283 MS.gene63758:CDS
ATAGTCATTGAAGAATTTCA+AGG 0.496242 2.1:+23262823 MS.gene63758:CDS
GTCTAGCTTGATTGCAGCCA+TGG 0.496694 2.1:+23263416 MS.gene63758:CDS
AAGCTAGACTTCCCAGTACC+AGG 0.497146 2.1:-23263402 None:intergenic
GGACTATTGAATGCAGTAGA+TGG 0.497808 2.1:+23264071 MS.gene63758:CDS
GTCCTAAACATTGTTCAATC+GGG 0.500773 2.1:+23262760 MS.gene63758:CDS
CCAAGCCAAGAAAATGGTCC+AGG 0.508568 2.1:+23264391 MS.gene63758:CDS
GATCTAGGCAGAGAAAATAT+AGG 0.509105 2.1:+23264452 MS.gene63758:CDS
CAACTATGATATCTATGACT+TGG 0.513130 2.1:+23263449 MS.gene63758:CDS
ATTTGCTTGTCCATTCTACC+AGG 0.513687 2.1:-23264176 None:intergenic
AGTTCGCATTAGCGCAATTG+AGG 0.515687 2.1:-23262710 None:intergenic
TTCATATTTGCCTTATGTGA+TGG 0.523892 2.1:+23263143 MS.gene63758:CDS
TGTATGGTCCTCCTGGTACT+GGG 0.528478 2.1:+23263391 MS.gene63758:CDS
AATTGATGAAGAGCTTCAAA+GGG 0.533769 2.1:+23263284 MS.gene63758:CDS
TCTGTTAAGACCTGGTAGAA+TGG 0.545715 2.1:+23264166 MS.gene63758:CDS
CTACCAGGTCTTAACAGAGC+AGG 0.546075 2.1:-23264161 None:intergenic
TATCACAAGTATAGAACGGT+TGG 0.555140 2.1:-23263526 None:intergenic
ACAAATTAAACAAGGAATTG+TGG 0.559399 2.1:+23263176 MS.gene63758:CDS
AAGAATTCGACATTATGATA+GGG 0.568700 2.1:+23263047 MS.gene63758:CDS
ACTCAAATGAATATAATCGT+TGG 0.570247 2.1:+23263211 MS.gene63758:CDS
ACATTATGATAGGGACTCCT+CGG 0.578511 2.1:+23263056 MS.gene63758:CDS
CTTGGATCTCACTAATGTTG+GGG 0.595649 2.1:+23263467 MS.gene63758:CDS
GATCCTGCTCTGTTAAGACC+TGG 0.601081 2.1:+23264158 MS.gene63758:CDS
GTTCATGTTCAGTAATGCAA+AGG 0.603050 2.1:-23264250 None:intergenic
TAGTCATTGAAGAATTTCAA+GGG 0.606257 2.1:+23262824 MS.gene63758:CDS
GAGTGTTGAAACTCATTGGA+TGG 0.615289 2.1:-23263257 None:intergenic
ACCGGGAAAGCTTGGAAACG+CGG 0.621197 2.1:+23263360 MS.gene63758:CDS
TCTTATTTCTGACACAGCCG+AGG 0.630279 2.1:-23263073 None:intergenic
CCTCTATCACAAGTATAGAA+CGG 0.641804 2.1:-23263530 None:intergenic
ATAGTTGAGATAGTTAGCCA+TGG 0.661661 2.1:-23263433 None:intergenic
GATGGTCTTTGGTCATGCTG+TGG 0.668698 2.1:+23264089 MS.gene63758:CDS
ACAATGTTTAGGACTTCACG+AGG 0.686927 2.1:-23262751 None:intergenic
AGAGCTAAACAAATTAAACA+AGG 0.691321 2.1:+23263168 MS.gene63758:CDS
AATTGCAGAAGAGTTAACAA+AGG 0.702150 2.1:+23264325 MS.gene63758:CDS
TCTTTGGTCATGCTGTGGAG+AGG 0.703389 2.1:+23264094 MS.gene63758:CDS
CTAGACTTCCCAGTACCAGG+AGG 0.750100 2.1:-23263399 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTAGCTGAAAAAATT+TGG + chr2.1:23263803-23263822 MS.gene63758:intron 15.0%
!! TCTTTCAAATAATTACAGTT+TGG - chr2.1:23263676-23263695 None:intergenic 20.0%
!!! AAAGAATTTTATAGAAGAAC+CGG + chr2.1:23263342-23263361 MS.gene63758:CDS 20.0%
!!! ATTAGTTTAAAATAGTTGAC+AGG - chr2.1:23264016-23264035 None:intergenic 20.0%
! AAGAATTCGACATTATGATA+GGG + chr2.1:23263047-23263066 MS.gene63758:CDS 25.0%
! AAGGGAAATTAAGAATGATT+TGG + chr2.1:23263302-23263321 MS.gene63758:CDS 25.0%
! ACAAATTAAACAAGGAATTG+TGG + chr2.1:23263176-23263195 MS.gene63758:CDS 25.0%
! ACTCAAATGAATATAATCGT+TGG + chr2.1:23263211-23263230 MS.gene63758:CDS 25.0%
! AGAGCTAAACAAATTAAACA+AGG + chr2.1:23263168-23263187 MS.gene63758:CDS 25.0%
! ATAGTCATTGAAGAATTTCA+AGG + chr2.1:23262823-23262842 MS.gene63758:CDS 25.0%
! ATGAAGAAGTTGTTCATAAT+GGG + chr2.1:23263592-23263611 MS.gene63758:CDS 25.0%
! TAAGTCATGTTGTTAACAAT+AGG - chr2.1:23263767-23263786 None:intergenic 25.0%
! TAGTCATTGAAGAATTTCAA+GGG + chr2.1:23262824-23262843 MS.gene63758:CDS 25.0%
! TTGAAATTCTTCAATGACTA+TGG - chr2.1:23262824-23262843 None:intergenic 25.0%
! TTTAATCATCTGCATGATTT+AGG + chr2.1:23263727-23263746 MS.gene63758:intron 25.0%
!! AAGAATTTTATAGAAGAACC+GGG + chr2.1:23263343-23263362 MS.gene63758:CDS 25.0%
!! CTTTTTATGTTCCTTAGATT+TGG - chr2.1:23262932-23262951 None:intergenic 25.0%
!!! CTTTTCTGCTTTTAAGAAAA+TGG + chr2.1:23264217-23264236 MS.gene63758:CDS 25.0%
AATTGATGAAGAGCTTCAAA+GGG + chr2.1:23263284-23263303 MS.gene63758:CDS 30.0%
AATTGCAGAAGAGTTAACAA+AGG + chr2.1:23264325-23264344 MS.gene63758:CDS 30.0%
ACAGGAACAAAAGAATATGT+TGG - chr2.1:23263998-23264017 None:intergenic 30.0%
AGTCCTAAACATTGTTCAAT+CGG + chr2.1:23262759-23262778 MS.gene63758:CDS 30.0%
AGTTAAAGCAACCAAATCTA+AGG + chr2.1:23262918-23262937 MS.gene63758:CDS 30.0%
ATGATGTCTGTTCATATCAT+GGG + chr2.1:23263954-23263973 MS.gene63758:intron 30.0%
ATTGGATGGTTGAACTTAAT+AGG - chr2.1:23263246-23263265 None:intergenic 30.0%
CAACTATGATATCTATGACT+TGG + chr2.1:23263449-23263468 MS.gene63758:CDS 30.0%
CAAGAATTCGACATTATGAT+AGG + chr2.1:23263046-23263065 MS.gene63758:CDS 30.0%
GAAAAGATTGAAGTTCTTCT+TGG + chr2.1:23264278-23264297 MS.gene63758:CDS 30.0%
GATGAAGAAGTTGTTCATAA+TGG + chr2.1:23263591-23263610 MS.gene63758:CDS 30.0%
TATGATGTCTGTTCATATCA+TGG + chr2.1:23263953-23263972 MS.gene63758:intron 30.0%
TTCATATTTGCCTTATGTGA+TGG + chr2.1:23263143-23263162 MS.gene63758:CDS 30.0%
! TTCTCGGTTTTGCAATTTTA+CGG - chr2.1:23263568-23263587 None:intergenic 30.0%
! TTTTCAACAGATGACACTTT+CGG + chr2.1:23264049-23264068 MS.gene63758:intron 30.0%
!! CTGAAAAAATTTGGTCTTGT+TGG + chr2.1:23263812-23263831 MS.gene63758:intron 30.0%
AATCATCTGCATGATTTAGG+CGG + chr2.1:23263730-23263749 MS.gene63758:intron 35.0%
ACTTGGATCTCACTAATGTT+GGG + chr2.1:23263466-23263485 MS.gene63758:CDS 35.0%
AGCAAAGAAGAACAAGATCT+AGG + chr2.1:23264437-23264456 MS.gene63758:CDS 35.0%
ATAGTTGAGATAGTTAGCCA+TGG - chr2.1:23263436-23263455 None:intergenic 35.0%
CAACTTCTTCATCTTCTTCT+CGG - chr2.1:23263584-23263603 None:intergenic 35.0%
CAATTGATGAAGAGCTTCAA+AGG + chr2.1:23263283-23263302 MS.gene63758:CDS 35.0%
CCTCTATCACAAGTATAGAA+CGG - chr2.1:23263533-23263552 None:intergenic 35.0%
CGTTGAATTGGCAAAAAAGA+TGG - chr2.1:23262792-23262811 None:intergenic 35.0%
CTAAATATTATGTCCTCGTG+CGG - chr2.1:23263792-23263811 None:intergenic 35.0%
GAATTTGACGTACATGAATC+TGG + chr2.1:23263694-23263713 MS.gene63758:intron 35.0%
GATCTAGGCAGAGAAAATAT+AGG + chr2.1:23264452-23264471 MS.gene63758:CDS 35.0%
GTCCTAAACATTGTTCAATC+GGG + chr2.1:23262760-23262779 MS.gene63758:CDS 35.0%
GTTCATGTTCAGTAATGCAA+AGG - chr2.1:23264253-23264272 None:intergenic 35.0%
TATCACAAGTATAGAACGGT+TGG - chr2.1:23263529-23263548 None:intergenic 35.0%
TCACAACAAATCACAAAGAC+AGG + chr2.1:23264132-23264151 MS.gene63758:CDS 35.0%
TGATGTCTGTTCATATCATG+GGG + chr2.1:23263955-23263974 MS.gene63758:intron 35.0%
TTTCAACAGATGACACTTTC+GGG + chr2.1:23264050-23264069 MS.gene63758:intron 35.0%
! AAGATTTTGTCGACAATGGT+TGG + chr2.1:23263836-23263855 MS.gene63758:intron 35.0%
! AGTTGCTTTAGTTCCTAGAT+AGG - chr2.1:23262884-23262903 None:intergenic 35.0%
! GACAAGAAACCAAGTTTTTG+AGG + chr2.1:23262849-23262868 MS.gene63758:CDS 35.0%
! TGGTAAGATTTTGTCGACAA+TGG + chr2.1:23263832-23263851 MS.gene63758:intron 35.0%
!! GAAGTGAGTGATGTTTTTGA+AGG + chr2.1:23262979-23262998 MS.gene63758:CDS 35.0%
!! TGTTCATAATGGGCACAATA+AGG + chr2.1:23263602-23263621 MS.gene63758:CDS 35.0%
!! TTGAAGGTGTTAGTGTAAAG+TGG + chr2.1:23262995-23263014 MS.gene63758:CDS 35.0%
AACTCTTCTGCAATTTCAGC+TGG - chr2.1:23264320-23264339 None:intergenic 40.0%
ACATTATGATAGGGACTCCT+CGG + chr2.1:23263056-23263075 MS.gene63758:CDS 40.0%
ATTCCTCCAAGCCAAGAAAA+TGG + chr2.1:23264385-23264404 MS.gene63758:CDS 40.0%
ATTTGCTTGTCCATTCTACC+AGG - chr2.1:23264179-23264198 None:intergenic 40.0%
CAAACTATGTACATACGACG+AGG + chr2.1:23263888-23263907 MS.gene63758:intron 40.0%
CCGTTCTATACTTGTGATAG+AGG + chr2.1:23263530-23263549 MS.gene63758:CDS 40.0%
CTTGGATCTCACTAATGTTG+GGG + chr2.1:23263467-23263486 MS.gene63758:CDS 40.0%
GACTTGGATCTCACTAATGT+TGG + chr2.1:23263465-23263484 MS.gene63758:CDS 40.0%
GGAATTTGATAAGGTCTTGC+AGG - chr2.1:23264370-23264389 None:intergenic 40.0%
GGACTATTGAATGCAGTAGA+TGG + chr2.1:23264071-23264090 MS.gene63758:CDS 40.0%
GTCCCGATTGAACAATGTTT+AGG - chr2.1:23262765-23262784 None:intergenic 40.0%
TCTGTTAAGACCTGGTAGAA+TGG + chr2.1:23264166-23264185 MS.gene63758:CDS 40.0%
TGAATGCAGTAGATGGTCTT+TGG + chr2.1:23264078-23264097 MS.gene63758:CDS 40.0%
TGTACATACGACGAGGTAAT+AGG + chr2.1:23263895-23263914 MS.gene63758:intron 40.0%
! ACAATGTTTAGGACTTCACG+AGG - chr2.1:23262754-23262773 None:intergenic 40.0%
! ATGATTTAGGCGGCGAATTT+TGG + chr2.1:23263740-23263759 MS.gene63758:intron 40.0%
! TGGCTTGGAGGAATTTGATA+AGG - chr2.1:23264379-23264398 None:intergenic 40.0%
! TTAGCTCTCTCCATCACATA+AGG - chr2.1:23263156-23263175 None:intergenic 40.0%
!! AAACGCGGTTACTTGTTGTA+TGG + chr2.1:23263375-23263394 MS.gene63758:CDS 40.0%
!! GAGTGTTGAAACTCATTGGA+TGG - chr2.1:23263260-23263279 None:intergenic 40.0%
!! GCAAGAGTGTTGAAACTCAT+TGG - chr2.1:23263264-23263283 None:intergenic 40.0%
AACATGACTTATACCGCACG+AGG + chr2.1:23263776-23263795 MS.gene63758:intron 45.0%
AGTTCGCATTAGCGCAATTG+AGG - chr2.1:23262713-23262732 None:intergenic 45.0%
ATAGAAGAACCGGGAAAGCT+TGG + chr2.1:23263352-23263371 MS.gene63758:CDS 45.0%
CTTCTCTCTTAGTCTCTTCC+TGG - chr2.1:23264412-23264431 None:intergenic 45.0%
CTTCTGCTGCCTCAAAAACT+TGG - chr2.1:23262861-23262880 None:intergenic 45.0%
TGGTGAATTGCGCGTTGAAT+TGG - chr2.1:23262804-23262823 None:intergenic 45.0%
! CTCTTCCTGGACCATTTTCT+TGG - chr2.1:23264399-23264418 None:intergenic 45.0%
! TCGACAATGGTTGGTCAAGT+TGG + chr2.1:23263845-23263864 MS.gene63758:intron 45.0%
!! CAAGCCAGTTTTATCTGCAG+TGG + chr2.1:23262687-23262706 MS.gene63758:CDS 45.0%
!! TACTTGTTGTATGGTCCTCC+TGG + chr2.1:23263384-23263403 MS.gene63758:CDS 45.0%
!! TCTTATTTCTGACACAGCCG+AGG - chr2.1:23263076-23263095 None:intergenic 45.0%
AAGCTAGACTTCCCAGTACC+AGG - chr2.1:23263405-23263424 None:intergenic 50.0%
CCAAGCCAAGAAAATGGTCC+AGG + chr2.1:23264391-23264410 MS.gene63758:CDS 50.0%
CTACCAGGTCTTAACAGAGC+AGG - chr2.1:23264164-23264183 None:intergenic 50.0%
GAGGCCACTGCAGATAAAAC+TGG - chr2.1:23262694-23262713 None:intergenic 50.0%
GATCCTGCTCTGTTAAGACC+TGG + chr2.1:23264158-23264177 MS.gene63758:CDS 50.0%
GATGGTCTTTGGTCATGCTG+TGG + chr2.1:23264089-23264108 MS.gene63758:CDS 50.0%
TCTTTGGTCATGCTGTGGAG+AGG + chr2.1:23264094-23264113 MS.gene63758:CDS 50.0%
TGTATGGTCCTCCTGGTACT+GGG + chr2.1:23263391-23263410 MS.gene63758:CDS 50.0%
TTGTATGGTCCTCCTGGTAC+TGG + chr2.1:23263390-23263409 MS.gene63758:CDS 50.0%
! CCTGGACCATTTTCTTGGCT+TGG - chr2.1:23264394-23264413 None:intergenic 50.0%
! GTCTAGCTTGATTGCAGCCA+TGG + chr2.1:23263416-23263435 MS.gene63758:CDS 50.0%
!!! GGACCATTTTCTTGGCTTGG+AGG - chr2.1:23264391-23264410 None:intergenic 50.0%
ACCGCGTTTCCAAGCTTTCC+CGG - chr2.1:23263364-23263383 None:intergenic 55.0%
ACCGGGAAAGCTTGGAAACG+CGG + chr2.1:23263360-23263379 MS.gene63758:CDS 55.0%
CTAGACTTCCCAGTACCAGG+AGG - chr2.1:23263402-23263421 None:intergenic 55.0%
GAGGCAGCAGAAGCCTATCT+AGG + chr2.1:23262868-23262887 MS.gene63758:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 23262655 23264487 23262655 ID=MS.gene63758
chr2.1 mRNA 23262655 23264487 23262655 ID=MS.gene63758.t1;Parent=MS.gene63758
chr2.1 exon 23262655 23263623 23262655 ID=MS.gene63758.t1.exon1;Parent=MS.gene63758.t1
chr2.1 CDS 23262655 23263623 23262655 ID=cds.MS.gene63758.t1;Parent=MS.gene63758.t1
chr2.1 exon 23264059 23264487 23264059 ID=MS.gene63758.t1.exon2;Parent=MS.gene63758.t1
chr2.1 CDS 23264059 23264487 23264059 ID=cds.MS.gene63758.t1;Parent=MS.gene63758.t1
Gene Sequence

>MS.gene63758

ATGACAATGATCTCAAAAATGTTTGATAGCAGCAAGCCAGTTTTATCTGCAGTGGCCTCAATTGCGCTAATGCGAACTGTCACAAATGAACTCATTCCTCGTGAAGTCCTAAACATTGTTCAATCGGGACTTCACCATCTTTTTTGCCAATTCAACGCGCAATTCACCATAGTCATTGAAGAATTTCAAGGGATGACAAGAAACCAAGTTTTTGAGGCAGCAGAAGCCTATCTAGGAACTAAAGCAACTGTCTCAGCAGAGAGAGTTAAAGCAACCAAATCTAAGGAACATAAAAAGCTTTCTTTCAATCTAGATAGAGATGAAGAAGTGAGTGATGTTTTTGAAGGTGTTAGTGTAAAGTGGAAACTAATATGCATACAAGTCGACTCATCAAGAATTCGACATTATGATAGGGACTCCTCGGCTGTGTCAGAAATAAGATCATATGAGCTAACTTTTCACAAGAAACACAAAAACAAGATCTTTGATTCATATTTGCCTTATGTGATGGAGAGAGCTAAACAAATTAAACAAGGAATTGTGGCACTTAAGATTCACTCAAATGAATATAATCGTTGGTGTCATGATCCTATTAAGTTCAACCATCCAATGAGTTTCAACACTCTTGCAATTGATGAAGAGCTTCAAAGGGAAATTAAGAATGATTTGGACAAATTTGTGAGTGCTAAAGAATTTTATAGAAGAACCGGGAAAGCTTGGAAACGCGGTTACTTGTTGTATGGTCCTCCTGGTACTGGGAAGTCTAGCTTGATTGCAGCCATGGCTAACTATCTCAACTATGATATCTATGACTTGGATCTCACTAATGTTGGGGATAATAAGAGCTTAAAGCAACTTATTCTCAGCATGTCCAACCGTTCTATACTTGTGATAGAGGATATTGATTGCTCCGTAAAATTGCAAAACCGAGAAGAAGATGAAGAAGTTGTTCATAATGGGCACAATAAGGTATGAATTTCTCTTTGAAAATATGATTCATCTTTGAGTTTCTTTCATACCAAACTGTAATTATTTGAAAGAATTTGACGTACATGAATCTGGTTTAGATCTATTTAATCATCTGCATGATTTAGGCGGCGAATTTTGGTCCTATTGTTAACAACATGACTTATACCGCACGAGGACATAATATTTAGCTGAAAAAATTTGGTCTTGTTGGTAAGATTTTGTCGACAATGGTTGGTCAAGTTGGTAAAATATGACATGTATTTTCAAACTATGTACATACGACGAGGTAATAGGTTCGAAATACTTACATATGTAAGTACGCTTTGATTTATGATGTCTGTTCATATCATGGGGACTAATTGAAAGCAATACCAACATATTCTTTTGTTCCTGTCAACTATTTTAAACTAATGTTTGTTGATACTTTTTCAACAGATGACACTTTCGGGACTATTGAATGCAGTAGATGGTCTTTGGTCATGCTGTGGAGAGGAACACATAATTGTTTTCACAACAAATCACAAAGACAGGCTTGATCCTGCTCTGTTAAGACCTGGTAGAATGGACAAGCAAATTCACTTGTCATATTGCAACTTTTCTGCTTTTAAGAAAATGGTTATCAACTACCTTTGCATTACTGAACATGAACTCTTTGAAAAGATTGAAGTTCTTCTTGGACAAGTTCAAGTTACTCCAGCTGAAATTGCAGAAGAGTTAACAAAGGATGTTGATGCTACAGAATGCCTGCAAGACCTTATCAAATTCCTCCAAGCCAAGAAAATGGTCCAGGAAGAGACTAAGAGAGAAGAAAATAGCAAAGAAGAACAAGATCTAGGCAGAGAAAATATAGGTCTATCTAAGTAG
Protein sequence

>MS.gene63758.t1

MTMISKMFDSSKPVLSAVASIALMRTVTNELIPREVLNIVQSGLHHLFCQFNAQFTIVIEEFQGMTRNQVFEAAEAYLGTKATVSAERVKATKSKEHKKLSFNLDRDEEVSDVFEGVSVKWKLICIQVDSSRIRHYDRDSSAVSEIRSYELTFHKKHKNKIFDSYLPYVMERAKQIKQGIVALKIHSNEYNRWCHDPIKFNHPMSFNTLAIDEELQREIKNDLDKFVSAKEFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLNYDIYDLDLTNVGDNKSLKQLILSMSNRSILVIEDIDCSVKLQNREEDEEVVHNGHNKMTLSGLLNAVDGLWSCCGEEHIIVFTTNHKDRLDPALLRPGRMDKQIHLSYCNFSAFKKMVINYLCITEHELFEKIEVLLGQVQVTPAEIAEELTKDVDATECLQDLIKFLQAKKMVQEETKREENSKEEQDLGRENIGLSK