AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060274.t1 MTR_1g106730 80.000 285 16 8 3 281 2 251 3.22e-146 410
MS.gene060274.t1 MTR_3g102980 44.444 279 100 11 4 275 1 231 1.74e-53 174
MS.gene060274.t1 MTR_1g018420 43.348 233 69 10 54 272 38 221 4.73e-44 149
MS.gene060274.t1 MTR_4g028610 31.863 204 70 6 40 178 136 335 5.08e-19 87.0
MS.gene060274.t1 MTR_0160s0030 31.343 201 90 5 54 229 164 341 1.22e-18 85.1
MS.gene060274.t1 MTR_1g067640 40.000 115 58 3 65 177 1 106 4.38e-17 77.4
MS.gene060274.t1 MTR_1g067650 34.940 166 91 6 64 227 1 151 5.96e-17 77.0
MS.gene060274.t1 MTR_4g027440 33.094 139 81 4 97 227 212 346 6.04e-17 80.1
MS.gene060274.t1 MTR_7g100100 48.101 79 39 2 105 183 34 110 9.67e-16 73.6
MS.gene060274.t1 MTR_7g023560 43.333 90 43 2 107 194 42 125 1.54e-15 73.6
MS.gene060274.t1 MTR_5g099040 39.091 110 28 3 107 181 132 237 9.15e-15 73.2
MS.gene060274.t1 MTR_1g115345 44.086 93 47 2 107 198 47 135 1.39e-14 71.2
MS.gene060274.t1 MTR_1g062780 30.846 201 91 6 59 226 81 266 4.11e-14 71.2
MS.gene060274.t1 MTR_5g062300 43.182 88 35 2 107 179 121 208 8.94e-14 69.7
MS.gene060274.t1 MTR_1g090703 28.704 216 99 8 55 226 97 301 2.07e-13 69.7
MS.gene060274.t1 MTR_1g067660 43.750 80 39 3 99 177 31 105 4.35e-13 66.2
MS.gene060274.t1 MTR_4g100860 32.597 181 82 7 56 229 158 305 6.03e-13 68.2
MS.gene060274.t1 MTR_4g057230 27.439 164 73 3 60 177 67 230 2.09e-12 66.2
MS.gene060274.t1 MTR_2g084315 31.163 215 98 6 55 226 67 274 5.52e-12 65.1
MS.gene060274.t1 MTR_2g096540 43.056 72 30 1 106 177 35 95 8.39e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060274.t1 AT1G27730 40.860 279 94 13 4 271 1 219 1.87e-46 155
MS.gene060274.t1 AT3G19580 41.597 238 90 10 54 281 66 264 1.09e-41 145
MS.gene060274.t1 AT3G19580 41.597 238 90 10 54 281 66 264 1.09e-41 145
MS.gene060274.t1 AT5G67450 37.795 254 109 10 4 225 1 237 4.22e-39 137
MS.gene060274.t1 AT3G49930 46.341 164 68 4 23 178 21 172 2.43e-38 134
MS.gene060274.t1 AT5G04340 38.934 244 96 9 40 271 30 232 4.08e-38 134
MS.gene060274.t1 AT5G43170 36.596 235 75 10 41 271 28 192 3.51e-29 110
MS.gene060274.t1 AT2G45120 36.571 175 90 4 54 226 146 301 2.19e-24 100
MS.gene060274.t1 AT3G60580 36.413 184 98 2 54 236 122 287 2.39e-24 99.8
MS.gene060274.t1 AT1G49900 41.176 136 69 4 106 232 194 327 7.65e-23 98.6
MS.gene060274.t1 AT1G49900 40.000 140 73 4 100 233 745 879 1.36e-22 97.8
MS.gene060274.t1 AT5G03510 33.824 204 95 7 55 229 69 261 4.54e-19 85.5
MS.gene060274.t1 AT1G02030 39.837 123 49 3 107 229 162 259 9.18e-19 84.0
MS.gene060274.t1 AT2G28200 31.220 205 92 5 55 226 73 261 9.08e-18 81.6
MS.gene060274.t1 AT1G02040 28.713 202 105 4 9 178 54 248 1.52e-17 81.6
MS.gene060274.t1 AT5G56200 47.312 93 30 3 105 178 342 434 4.55e-17 81.3
MS.gene060274.t1 AT5G04390 32.934 167 67 5 55 182 96 256 2.75e-16 78.2
MS.gene060274.t1 AT2G17180 34.043 141 56 5 53 179 118 235 2.00e-15 74.7
MS.gene060274.t1 AT2G37430 37.815 119 55 3 59 177 17 116 2.82e-15 72.8
MS.gene060274.t1 AT3G53600 37.500 112 60 2 75 182 15 120 9.48e-15 71.2
MS.gene060274.t1 AT2G28710 50.704 71 29 1 107 177 36 100 2.88e-14 69.3
MS.gene060274.t1 AT4G35280 45.238 84 34 3 107 179 164 246 7.92e-13 67.8
MS.gene060274.t1 AT5G59820 46.479 71 31 1 107 177 41 104 1.24e-12 65.1
MS.gene060274.t1 AT3G46070 44.444 72 40 0 106 177 37 108 1.31e-12 65.1
MS.gene060274.t1 AT3G10470 34.314 102 38 2 105 177 182 283 1.52e-12 67.4
MS.gene060274.t1 AT3G46080 45.833 72 37 1 106 177 38 107 1.91e-12 64.3
MS.gene060274.t1 AT3G46090 47.945 73 32 2 106 177 41 108 9.07e-12 62.8
MS.gene060274.t1 AT5G61470 40.000 75 39 2 105 178 228 297 2.10e-11 63.5

Find 72 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATTAATCTTGGTCTTCT+TGG 0.185197 1.2:-79363039 None:intergenic
TACCTCTTGTTCATTGATTC+CGG 0.301905 1.2:-79363069 None:intergenic
GATTCATGAACGTGAGTCTT+TGG 0.319967 1.2:+79362410 MS.gene060274:CDS
GGCGAGATTAACATTAATCT+TGG 0.353029 1.2:-79363048 None:intergenic
CGTTGCCGGTAACCGCTAAA+AGG 0.357837 1.2:+79363099 MS.gene060274:CDS
TTCTGGTCTCCGGTTGGATT+CGG 0.364389 1.2:+79363005 MS.gene060274:CDS
CAGCGCCTCTCACTGAATTC+TGG 0.379619 1.2:+79362988 MS.gene060274:CDS
GGAACACTCGTGCATCTTAC+CGG 0.381325 1.2:-79362769 None:intergenic
CGATCAAAACGGCCGCGTAT+TGG 0.396652 1.2:+79362444 MS.gene060274:CDS
AGAGGTAGAAAGTCCGTTGC+CGG 0.419568 1.2:+79363085 MS.gene060274:CDS
TGATGAAGATTTACGGTGGC+TGG 0.425019 1.2:-79362691 None:intergenic
GACTGTCTCGTTGACGGCGG+TGG 0.426617 1.2:-79362724 None:intergenic
TCTCGTTGACGGCGGTGGAT+TGG 0.428167 1.2:-79362719 None:intergenic
CCACCAAGTGCTTGATAAGA+TGG 0.436761 1.2:-79362660 None:intergenic
AGGAAAACTCTTGTGACAAA+TGG 0.437929 1.2:-79362790 None:intergenic
GCTTTGAATTCTTCTCCAAC+AGG 0.439849 1.2:+79362339 MS.gene060274:CDS
TTTCCTACCGGTCAAGCTCT+TGG 0.452673 1.2:+79362807 MS.gene060274:CDS
TGATTCAGCTTCACCGGTGA+TGG 0.483480 1.2:-79362609 None:intergenic
CTTGTTGTTGTTTGAAGCGT+GGG 0.484440 1.2:-79362377 None:intergenic
GTGTCCACCAAGAGCTTGAC+CGG 0.489744 1.2:-79362814 None:intergenic
TGTTGTTTGAAGCGTGGGAA+TGG 0.492509 1.2:-79362372 None:intergenic
CCATCTTATCAAGCACTTGG+TGG 0.494016 1.2:+79362660 MS.gene060274:CDS
GTCAAATCCACGGTGGCTGA+TGG 0.494460 1.2:-79362955 None:intergenic
TTCCCATCTTATCAAGCACT+TGG 0.496098 1.2:+79362657 MS.gene060274:CDS
CCTACCGGTCAAGCTCTTGG+TGG 0.502683 1.2:+79362810 MS.gene060274:CDS
TCTCCACCACCGAATCCAAC+CGG 0.506094 1.2:-79363014 None:intergenic
TGTTACTGTTGTTATGATTG+TGG 0.508189 1.2:-79362875 None:intergenic
ATCACAGTCTCCGACGGCGG+CGG 0.508871 1.2:+79362921 MS.gene060274:CDS
GGAGGACGCGCCGCCGCCGT+CGG 0.514572 1.2:-79362931 None:intergenic
ACTGAATTCTGGTCTCCGGT+TGG 0.515567 1.2:+79362999 MS.gene060274:CDS
TCTCACTGAATTCTGGTCTC+CGG 0.517672 1.2:+79362995 MS.gene060274:CDS
GATATTCTTCTTCAGTGGGT+GGG 0.517674 1.2:-79362470 None:intergenic
CAGAATTCAGTGAGAGGCGC+TGG 0.519958 1.2:-79362987 None:intergenic
AGATATTCTTCTTCAGTGGG+TGG 0.522083 1.2:-79362471 None:intergenic
TGTTTCATCATTGTCTTCAC+CGG 0.523785 1.2:-79363135 None:intergenic
TCTCCGGTTGGATTCGGTGG+TGG 0.527155 1.2:+79363011 MS.gene060274:CDS
CACCGGTGATTCAGCTTCAC+CGG 0.531534 1.2:-79362615 None:intergenic
AAGATTAATGTTAATCTCGC+CGG 0.533608 1.2:+79363050 MS.gene060274:CDS
AACAGAGACTGTCTCGTTGA+CGG 0.534230 1.2:-79362730 None:intergenic
GTTGCAGTCGCCGCCATCAC+CGG 0.540954 1.2:+79362596 MS.gene060274:CDS
TCCACGGTGGCTGATGGAGG+AGG 0.543367 1.2:-79362949 None:intergenic
CACCGGTGAAGCTGAATCAC+CGG 0.548157 1.2:+79362613 MS.gene060274:CDS
AGCGAGATATTCTTCTTCAG+TGG 0.552258 1.2:-79362475 None:intergenic
GCGAGATATTCTTCTTCAGT+GGG 0.560158 1.2:-79362474 None:intergenic
TGGTCTCCGGTTGGATTCGG+TGG 0.560623 1.2:+79363008 MS.gene060274:CDS
GGTGATGGCGGCGACTGCAA+CGG 0.560799 1.2:-79362594 None:intergenic
CACAAACGATGTCACTATGA+AGG 0.562849 1.2:+79362834 MS.gene060274:CDS
TGAGAGGCGCTGGTAAATTG+AGG 0.568160 1.2:-79362977 None:intergenic
GTTTCCGTGTCAACGGTCAC+CGG 0.569344 1.2:+79362750 MS.gene060274:CDS
CACCAAGTGCTTGATAAGAT+GGG 0.573814 1.2:-79362659 None:intergenic
AGTCTCTGTTTCCGTGTCAA+CGG 0.584449 1.2:+79362743 MS.gene060274:CDS
TTCAGCTTCACCGGTGATGG+CGG 0.589012 1.2:-79362606 None:intergenic
CTTTGTTACAAACACTGCAC+CGG 0.591435 1.2:-79362632 None:intergenic
CGCCGGAATCAATGAACAAG+AGG 0.596526 1.2:+79363067 MS.gene060274:CDS
AAATCCACGGTGGCTGATGG+AGG 0.597416 1.2:-79362952 None:intergenic
AATGGAATAGTAGCTCCTGT+TGG 0.598789 1.2:-79362354 None:intergenic
CAAAGCTTGTAACTCTAAAG+CGG 0.606121 1.2:-79362322 None:intergenic
CCACCAAGAGCTTGACCGGT+AGG 0.606411 1.2:-79362810 None:intergenic
CTTACCGGTGACCGTTGACA+CGG 0.608002 1.2:-79362754 None:intergenic
TCTTGTTGTTGTTTGAAGCG+TGG 0.615448 1.2:-79362378 None:intergenic
GGAATCACAGTCTCCGACGG+CGG 0.622544 1.2:+79362918 MS.gene060274:CDS
AGTGGAATCACAGTCTCCGA+CGG 0.627679 1.2:+79362915 MS.gene060274:CDS
AGCAACGTCAATAACAGCAG+TGG 0.636514 1.2:+79362897 MS.gene060274:CDS
TCCTCCTCCATCAGCCACCG+TGG 0.642688 1.2:+79362948 MS.gene060274:CDS
GTAAATTGAGGTCAAATCCA+CGG 0.651066 1.2:-79362965 None:intergenic
GGAGACCAGAATTCAGTGAG+AGG 0.681730 1.2:-79362993 None:intergenic
TGCTTTGAGAGAGCATGATG+AGG 0.697077 1.2:-79362503 None:intergenic
GTGGGTGGGTTACCAATACG+CGG 0.720792 1.2:-79362456 None:intergenic
TTTCTGATGAAGATTTACGG+TGG 0.727105 1.2:-79362695 None:intergenic
AGAGACTGTCTCGTTGACGG+CGG 0.729919 1.2:-79362727 None:intergenic
TCTTCACCGGAAAACAAACG+CGG 0.774869 1.2:-79363122 None:intergenic
AATTGAGGTCAAATCCACGG+TGG 0.794635 1.2:-79362962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAAAAAGAAACGATCAAAA+CGG + chr1.2:79362433-79362452 MS.gene060274:CDS 25.0%
AAGATTAATGTTAATCTCGC+CGG + chr1.2:79363050-79363069 MS.gene060274:CDS 30.0%
TGTTACTGTTGTTATGATTG+TGG - chr1.2:79362878-79362897 None:intergenic 30.0%
! AACATTAATCTTGGTCTTCT+TGG - chr1.2:79363042-79363061 None:intergenic 30.0%
! GGTTTTCTGATGAAGATTTA+CGG - chr1.2:79362701-79362720 None:intergenic 30.0%
AGGAAAACTCTTGTGACAAA+TGG - chr1.2:79362793-79362812 None:intergenic 35.0%
GGCGAGATTAACATTAATCT+TGG - chr1.2:79363051-79363070 None:intergenic 35.0%
GTAAATTGAGGTCAAATCCA+CGG - chr1.2:79362968-79362987 None:intergenic 35.0%
TGTTTCATCATTGTCTTCAC+CGG - chr1.2:79363138-79363157 None:intergenic 35.0%
TTTCTGATGAAGATTTACGG+TGG - chr1.2:79362698-79362717 None:intergenic 35.0%
! CAAAGCTTGTAACTCTAAAG+CGG - chr1.2:79362325-79362344 None:intergenic 35.0%
!! TACCTCTTGTTCATTGATTC+CGG - chr1.2:79363072-79363091 None:intergenic 35.0%
AATGGAATAGTAGCTCCTGT+TGG - chr1.2:79362357-79362376 None:intergenic 40.0%
AGATATTCTTCTTCAGTGGG+TGG - chr1.2:79362474-79362493 None:intergenic 40.0%
AGCGAGATATTCTTCTTCAG+TGG - chr1.2:79362478-79362497 None:intergenic 40.0%
CACAAACGATGTCACTATGA+AGG + chr1.2:79362834-79362853 MS.gene060274:CDS 40.0%
CACCAAGTGCTTGATAAGAT+GGG - chr1.2:79362662-79362681 None:intergenic 40.0%
CTTTGTTACAAACACTGCAC+CGG - chr1.2:79362635-79362654 None:intergenic 40.0%
GATATTCTTCTTCAGTGGGT+GGG - chr1.2:79362473-79362492 None:intergenic 40.0%
GATTCATGAACGTGAGTCTT+TGG + chr1.2:79362410-79362429 MS.gene060274:CDS 40.0%
GCGAGATATTCTTCTTCAGT+GGG - chr1.2:79362477-79362496 None:intergenic 40.0%
TTCCCATCTTATCAAGCACT+TGG + chr1.2:79362657-79362676 MS.gene060274:CDS 40.0%
! GCTTTGAATTCTTCTCCAAC+AGG + chr1.2:79362339-79362358 MS.gene060274:CDS 40.0%
! TGTCACAAGAGTTTTCCTAC+CGG + chr1.2:79362795-79362814 MS.gene060274:CDS 40.0%
!! CTTGTTGTTGTTTGAAGCGT+GGG - chr1.2:79362380-79362399 None:intergenic 40.0%
!! TCTTGTTGTTGTTTGAAGCG+TGG - chr1.2:79362381-79362400 None:intergenic 40.0%
AACAGAGACTGTCTCGTTGA+CGG - chr1.2:79362733-79362752 None:intergenic 45.0%
AATTGAGGTCAAATCCACGG+TGG - chr1.2:79362965-79362984 None:intergenic 45.0%
AGCAACGTCAATAACAGCAG+TGG + chr1.2:79362897-79362916 MS.gene060274:CDS 45.0%
AGTCTCTGTTTCCGTGTCAA+CGG + chr1.2:79362743-79362762 MS.gene060274:CDS 45.0%
CCACCAAGTGCTTGATAAGA+TGG - chr1.2:79362663-79362682 None:intergenic 45.0%
CCATCTTATCAAGCACTTGG+TGG + chr1.2:79362660-79362679 MS.gene060274:CDS 45.0%
TCTCACTGAATTCTGGTCTC+CGG + chr1.2:79362995-79363014 MS.gene060274:CDS 45.0%
TCTTCACCGGAAAACAAACG+CGG - chr1.2:79363125-79363144 None:intergenic 45.0%
TGATGAAGATTTACGGTGGC+TGG - chr1.2:79362694-79362713 None:intergenic 45.0%
! AAACAAACGCGGCCTTTTAG+CGG - chr1.2:79363114-79363133 None:intergenic 45.0%
! TGTTGTTTGAAGCGTGGGAA+TGG - chr1.2:79362375-79362394 None:intergenic 45.0%
!! AAAAGGCCGCGTTTGTTTTC+CGG + chr1.2:79363116-79363135 MS.gene060274:CDS 45.0%
!! TGCTTTGAGAGAGCATGATG+AGG - chr1.2:79362506-79362525 None:intergenic 45.0%
ACTGAATTCTGGTCTCCGGT+TGG + chr1.2:79362999-79363018 MS.gene060274:CDS 50.0%
AGAGGTAGAAAGTCCGTTGC+CGG + chr1.2:79363085-79363104 MS.gene060274:CDS 50.0%
AGTGGAATCACAGTCTCCGA+CGG + chr1.2:79362915-79362934 MS.gene060274:CDS 50.0%
CGCCGGAATCAATGAACAAG+AGG + chr1.2:79363067-79363086 MS.gene060274:CDS 50.0%
GGAACACTCGTGCATCTTAC+CGG - chr1.2:79362772-79362791 None:intergenic 50.0%
GGAGACCAGAATTCAGTGAG+AGG - chr1.2:79362996-79363015 None:intergenic 50.0%
TGATTCAGCTTCACCGGTGA+TGG - chr1.2:79362612-79362631 None:intergenic 50.0%
TTCTGGTCTCCGGTTGGATT+CGG + chr1.2:79363005-79363024 MS.gene060274:CDS 50.0%
TTTCCTACCGGTCAAGCTCT+TGG + chr1.2:79362807-79362826 MS.gene060274:CDS 50.0%
! CTTTTAGCGGTTACCGGCAA+CGG - chr1.2:79363101-79363120 None:intergenic 50.0%
!! TGAGAGGCGCTGGTAAATTG+AGG - chr1.2:79362980-79362999 None:intergenic 50.0%
AAATCCACGGTGGCTGATGG+AGG - chr1.2:79362955-79362974 None:intergenic 55.0%
AGAGACTGTCTCGTTGACGG+CGG - chr1.2:79362730-79362749 None:intergenic 55.0%
CACCGGTGAAGCTGAATCAC+CGG + chr1.2:79362613-79362632 MS.gene060274:CDS 55.0%
CACCGGTGATTCAGCTTCAC+CGG - chr1.2:79362618-79362637 None:intergenic 55.0%
CAGCGCCTCTCACTGAATTC+TGG + chr1.2:79362988-79363007 MS.gene060274:CDS 55.0%
CGATCAAAACGGCCGCGTAT+TGG + chr1.2:79362444-79362463 MS.gene060274:CDS 55.0%
CGTTGCCGGTAACCGCTAAA+AGG + chr1.2:79363099-79363118 MS.gene060274:CDS 55.0%
CTTACCGGTGACCGTTGACA+CGG - chr1.2:79362757-79362776 None:intergenic 55.0%
GTCAAATCCACGGTGGCTGA+TGG - chr1.2:79362958-79362977 None:intergenic 55.0%
GTGTCCACCAAGAGCTTGAC+CGG - chr1.2:79362817-79362836 None:intergenic 55.0%
GTTTCCGTGTCAACGGTCAC+CGG + chr1.2:79362750-79362769 MS.gene060274:CDS 55.0%
TCTCCACCACCGAATCCAAC+CGG - chr1.2:79363017-79363036 None:intergenic 55.0%
! CAGAATTCAGTGAGAGGCGC+TGG - chr1.2:79362990-79363009 None:intergenic 55.0%
! TTCAGCTTCACCGGTGATGG+CGG - chr1.2:79362609-79362628 None:intergenic 55.0%
!! GTGGGTGGGTTACCAATACG+CGG - chr1.2:79362459-79362478 None:intergenic 55.0%
CCACCAAGAGCTTGACCGGT+AGG - chr1.2:79362813-79362832 None:intergenic 60.0%
CCTACCGGTCAAGCTCTTGG+TGG + chr1.2:79362810-79362829 MS.gene060274:CDS 60.0%
GGAATCACAGTCTCCGACGG+CGG + chr1.2:79362918-79362937 MS.gene060274:CDS 60.0%
TCTCGTTGACGGCGGTGGAT+TGG - chr1.2:79362722-79362741 None:intergenic 60.0%
TGGTCTCCGGTTGGATTCGG+TGG + chr1.2:79363008-79363027 MS.gene060274:CDS 60.0%
! CGCGGCCTTTTAGCGGTTAC+CGG - chr1.2:79363107-79363126 None:intergenic 60.0%
!! TCTCCGGTTGGATTCGGTGG+TGG + chr1.2:79363011-79363030 MS.gene060274:CDS 60.0%
ATCACAGTCTCCGACGGCGG+CGG + chr1.2:79362921-79362940 MS.gene060274:CDS 65.0%
GACTGTCTCGTTGACGGCGG+TGG - chr1.2:79362727-79362746 None:intergenic 65.0%
GTTGCAGTCGCCGCCATCAC+CGG + chr1.2:79362596-79362615 MS.gene060274:CDS 65.0%
TCCACGGTGGCTGATGGAGG+AGG - chr1.2:79362952-79362971 None:intergenic 65.0%
TCCTCCTCCATCAGCCACCG+TGG + chr1.2:79362948-79362967 MS.gene060274:CDS 65.0%
!! GGTGATGGCGGCGACTGCAA+CGG - chr1.2:79362597-79362616 None:intergenic 65.0%
! GGAGGACGCGCCGCCGCCGT+CGG - chr1.2:79362934-79362953 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 79362318 79363163 79362318 ID=MS.gene060274
chr1.2 mRNA 79362318 79363163 79362318 ID=MS.gene060274.t1;Parent=MS.gene060274
chr1.2 exon 79362318 79363163 79362318 ID=MS.gene060274.t1.exon1;Parent=MS.gene060274.t1
chr1.2 CDS 79362318 79363163 79362318 ID=cds.MS.gene060274.t1;Parent=MS.gene060274.t1
Gene Sequence

>MS.gene060274

ATGGCCGCTTTAGAGTTACAAGCTTTGAATTCTTCTCCAACAGGAGCTACTATTCCATTCCCACGCTTCAAACAACAACAAGAAAATCAAGAGATTCATGAACGTGAGTCTTTGGTCAAAAAGAAACGATCAAAACGGCCGCGTATTGGTAACCCACCCACTGAAGAAGAATATCTCGCTCTCTGCCTCATCATGCTCTCTCAAAGCAACAACAACGAAAAAATCAACATCTCTGAATCTCATCATCAAATTGAATCATCATCTTCACAACAACAACCGTTGCAGTCGCCGCCATCACCGGTGAAGCTGAATCACCGGTGCAGTGTTTGTAACAAAGCTTTCCCATCTTATCAAGCACTTGGTGGTCACAAAGCCAGCCACCGTAAATCTTCATCAGAAAACCAATCCACCGCCGTCAACGAGACAGTCTCTGTTTCCGTGTCAACGGTCACCGGTAAGATGCACGAGTGTTCCATTTGTCACAAGAGTTTTCCTACCGGTCAAGCTCTTGGTGGACACAAACGATGTCACTATGAAGGAGTCATCAACAACAACAACCACAATCATAACAACAGTAACAGCAACGTCAATAACAGCAGTGGAATCACAGTCTCCGACGGCGGCGGCGCGTCCTCCTCCATCAGCCACCGTGGATTTGACCTCAATTTACCAGCGCCTCTCACTGAATTCTGGTCTCCGGTTGGATTCGGTGGTGGAGATTCCAAGAAGACCAAGATTAATGTTAATCTCGCCGGAATCAATGAACAAGAGGTAGAAAGTCCGTTGCCGGTAACCGCTAAAAGGCCGCGTTTGTTTTCCGGTGAAGACAATGATGAAACAGTGTAG
Protein sequence

>MS.gene060274.t1

MAALELQALNSSPTGATIPFPRFKQQQENQEIHERESLVKKKRSKRPRIGNPPTEEEYLALCLIMLSQSNNNEKINISESHHQIESSSSQQQPLQSPPSPVKLNHRCSVCNKAFPSYQALGGHKASHRKSSSENQSTAVNETVSVSVSTVTGKMHECSICHKSFPTGQALGGHKRCHYEGVINNNNHNHNNSNSNVNNSSGITVSDGGGASSSISHRGFDLNLPAPLTEFWSPVGFGGGDSKKTKINVNLAGINEQEVESPLPVTAKRPRLFSGEDNDETV