Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060423.t1 | XP_003622202.1 | 97.4 | 498 | 13 | 0 | 1 | 498 | 1 | 498 | 8.40E-278 | 965.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060423.t1 | Q94IG7 | 68.9 | 486 | 148 | 2 | 10 | 493 | 39 | 523 | 1.1e-198 | 694.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060423.t1 | G7L3C0 | 97.4 | 498 | 13 | 0 | 1 | 498 | 1 | 498 | 6.0e-278 | 965.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060423 | MS.gene62573 | 0.85594 | 4.50E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060423.t1 | MTR_7g031310 | 97.390 | 498 | 13 | 0 | 1 | 498 | 1 | 498 | 0.0 | 998 |
| MS.gene060423.t1 | MTR_1g085730 | 29.495 | 495 | 312 | 15 | 16 | 493 | 73 | 547 | 3.60e-50 | 180 |
| MS.gene060423.t1 | MTR_1g085730 | 31.525 | 295 | 180 | 9 | 16 | 303 | 73 | 352 | 5.12e-30 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060423.t1 | AT5G14220 | 69.057 | 488 | 144 | 3 | 14 | 497 | 17 | 501 | 0.0 | 708 |
| MS.gene060423.t1 | AT5G14220 | 69.057 | 488 | 144 | 3 | 14 | 497 | 65 | 549 | 0.0 | 706 |
| MS.gene060423.t1 | AT5G14220 | 65.714 | 490 | 131 | 3 | 12 | 497 | 15 | 471 | 0.0 | 656 |
| MS.gene060423.t1 | AT5G14220 | 67.416 | 445 | 138 | 3 | 57 | 497 | 2 | 443 | 0.0 | 635 |
| MS.gene060423.t1 | AT5G14220 | 67.416 | 445 | 138 | 3 | 57 | 497 | 2 | 443 | 0.0 | 635 |
| MS.gene060423.t1 | AT4G01690 | 28.287 | 502 | 307 | 15 | 16 | 492 | 59 | 532 | 1.27e-44 | 165 |
| MS.gene060423.t1 | AT4G01690 | 26.908 | 498 | 288 | 14 | 16 | 492 | 59 | 501 | 6.55e-38 | 145 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCCAGAGCTATGGAATT+TGG | 0.164490 | 7.1:+66329701 | MS.gene060423:CDS |
| CCTTTATATGGTCATAACTA+TGG | 0.165882 | 7.1:+66333469 | MS.gene060423:CDS |
| GCTTTACAATCCAGCGTATT+TGG | 0.185895 | 7.1:+66330454 | MS.gene060423:CDS |
| CTTAATAACCACCTTTAAAA+AGG | 0.225514 | 7.1:+66332718 | MS.gene060423:CDS |
| AGATTCTTCGAACGCCATTT+TGG | 0.225642 | 7.1:+66328675 | MS.gene060423:CDS |
| CATCTCTATACCACCTTCAT+AGG | 0.233270 | 7.1:+66332963 | MS.gene060423:CDS |
| ACAATTGCAGCCATTTCTTC+TGG | 0.259910 | 7.1:+66333434 | MS.gene060423:intron |
| AGACATCCAGATCACGAAAA+AGG | 0.265116 | 7.1:+66332375 | MS.gene060423:CDS |
| TCTTGAAGCAATTGACAAAA+TGG | 0.277093 | 7.1:+66333498 | MS.gene060423:CDS |
| GAAAATGAAATAGAGGTTAA+AGG | 0.282471 | 7.1:+66327204 | MS.gene060423:CDS |
| AGTAAAGGCTTTCCTTTATA+TGG | 0.287943 | 7.1:+66333457 | MS.gene060423:CDS |
| TTGTAAAGCCCCTGCTATAA+TGG | 0.295256 | 7.1:-66330440 | None:intergenic |
| AAGAGACCACTGGAGGGTTT+TGG | 0.296122 | 7.1:+66332749 | MS.gene060423:CDS |
| TTTCTCGAGATGGGCTAGTC+TGG | 0.296131 | 7.1:+66326480 | MS.gene060423:CDS |
| AAACTCACAGAAAGAGATTC+AGG | 0.299826 | 7.1:-66329553 | None:intergenic |
| CCTTCAAAAGAGCAACAAAA+TGG | 0.315816 | 7.1:+66332782 | MS.gene060423:CDS |
| CATATACCTCTTTCCCAAAA+TGG | 0.334289 | 7.1:-66328689 | None:intergenic |
| TTCTCCAAATTCCATAGCTC+TGG | 0.342861 | 7.1:-66329705 | None:intergenic |
| AGTAGTGCAGCAGGATTTGC+AGG | 0.374983 | 7.1:-66327720 | None:intergenic |
| GAAGTTCCCCATCGCAAAAC+TGG | 0.378508 | 7.1:+66330794 | MS.gene060423:CDS |
| CTGTTGTTGGTGCTGGTGTA+AGG | 0.394896 | 7.1:+66323866 | MS.gene060423:CDS |
| AAAGCTGTTGGGAGCGGAGG+GGG | 0.398693 | 7.1:+66333340 | MS.gene060423:CDS |
| TAAGACTGAGAGTGGTAAAT+AGG | 0.402238 | 7.1:-66332699 | None:intergenic |
| AGGTTTGGTTCCATTATAGC+AGG | 0.412676 | 7.1:+66330430 | MS.gene060423:intron |
| GTTAAAAGAGTTGCTGTTGT+TGG | 0.430484 | 7.1:+66323853 | MS.gene060423:CDS |
| TGTTGTCTGAAGCATTGTTT+AGG | 0.430646 | 7.1:-66334736 | None:intergenic |
| AGCCTTTACTCCAGAAGAAA+TGG | 0.436498 | 7.1:-66333444 | None:intergenic |
| CCATAGTTATGACCATATAA+AGG | 0.439671 | 7.1:-66333469 | None:intergenic |
| AGCTATGGAATTTGGAGAAA+AGG | 0.440643 | 7.1:+66329709 | MS.gene060423:CDS |
| ATATCACTGGGTGCTCGATC+TGG | 0.441880 | 7.1:-66332939 | None:intergenic |
| AAAGGTTTGATTGATGCTCT+TGG | 0.449228 | 7.1:+66327222 | MS.gene060423:CDS |
| CTAGCAAGACATACCAGAAC+TGG | 0.452360 | 7.1:-66327566 | None:intergenic |
| GTTGGAGGTACAAGTGCTGC+AGG | 0.456983 | 7.1:+66329529 | MS.gene060423:CDS |
| ATCTCTATACCACCTTCATA+GGG | 0.462355 | 7.1:+66332964 | MS.gene060423:CDS |
| AGCCATTTCTTCTGGAGTAA+AGG | 0.466548 | 7.1:+66333442 | MS.gene060423:intron |
| CTTTATATGGTCATAACTAT+GGG | 0.469339 | 7.1:+66333470 | MS.gene060423:CDS |
| TTGCTCTTCAGTAGTGCAGC+AGG | 0.472490 | 7.1:-66327729 | None:intergenic |
| AGAGTTGCTGTTGTTGGTGC+TGG | 0.474709 | 7.1:+66323859 | MS.gene060423:CDS |
| TTCTCGAGATGGGCTAGTCT+GGG | 0.477873 | 7.1:+66326481 | MS.gene060423:CDS |
| AGAAAGCTGTTGGGAGCGGA+GGG | 0.479152 | 7.1:+66333338 | MS.gene060423:CDS |
| ACTTCTTTCTGCACAATCAA+AGG | 0.479775 | 7.1:+66327752 | MS.gene060423:CDS |
| TCCATAGCTCTGGGAAAGAA+TGG | 0.480104 | 7.1:-66329695 | None:intergenic |
| CCGCTCCCAACAGCTTTCTC+AGG | 0.488618 | 7.1:-66333334 | None:intergenic |
| TTCTGACCTGAGAAAGCTGT+TGG | 0.492675 | 7.1:+66333328 | MS.gene060423:CDS |
| TCTCCAAATTCCATAGCTCT+GGG | 0.495582 | 7.1:-66329704 | None:intergenic |
| GTTGGTAGAGCAATTGCCTC+GGG | 0.495658 | 7.1:+66334683 | MS.gene060423:CDS |
| GAGAAAGCTGTTGGGAGCGG+AGG | 0.496482 | 7.1:+66333337 | MS.gene060423:CDS |
| GCAGACTGAAAATGAAATAG+AGG | 0.497637 | 7.1:+66327197 | MS.gene060423:intron |
| GCCATTCTTTCCCAGAGCTA+TGG | 0.506378 | 7.1:+66329694 | MS.gene060423:CDS |
| CTAAGAACTGTTTCTCGAGA+TGG | 0.506572 | 7.1:+66326470 | MS.gene060423:CDS |
| TTTATGACACAGGCTCCTCT+TGG | 0.512858 | 7.1:+66332343 | MS.gene060423:intron |
| GGTATAGAGATGCATATCAC+TGG | 0.515192 | 7.1:-66332952 | None:intergenic |
| GCACTTGTACCTCCAACAAA+AGG | 0.524762 | 7.1:-66329523 | None:intergenic |
| TAAGAACTGTTTCTCGAGAT+GGG | 0.527534 | 7.1:+66326471 | MS.gene060423:CDS |
| ATGGCCTCCTCTGCAAAAGA+TGG | 0.528542 | 7.1:+66323656 | MS.gene060423:CDS |
| CCTGAGAAAGCTGTTGGGAG+CGG | 0.532148 | 7.1:+66333334 | MS.gene060423:CDS |
| GGTTTGGTTCCATTATAGCA+GGG | 0.532807 | 7.1:+66330431 | MS.gene060423:intron |
| GATACATTGTGCAAAGAGCT+TGG | 0.533819 | 7.1:+66330715 | MS.gene060423:CDS |
| AAGAAAATTAAGTGGGAAGC+GGG | 0.535220 | 7.1:-66332401 | None:intergenic |
| TCTGACCTGAGAAAGCTGTT+GGG | 0.538599 | 7.1:+66333329 | MS.gene060423:CDS |
| TGACATTAAATTAAATGCGA+AGG | 0.544078 | 7.1:+66330744 | MS.gene060423:CDS |
| AACTCACAGAAAGAGATTCA+GGG | 0.549159 | 7.1:-66329552 | None:intergenic |
| ACCTCGGGAAGAAAATTAAG+TGG | 0.552598 | 7.1:-66332409 | None:intergenic |
| AGTTGGTAGAGCAATTGCCT+CGG | 0.554244 | 7.1:+66334682 | MS.gene060423:CDS |
| TTTGAAGCTGATGGAAGAGC+TGG | 0.554280 | 7.1:+66326440 | MS.gene060423:CDS |
| GTAACTGTATTTGAAGCTGA+TGG | 0.558846 | 7.1:+66326431 | MS.gene060423:CDS |
| GTCTGAAGCATTGTTTAGGT+AGG | 0.565659 | 7.1:-66334732 | None:intergenic |
| CTGATGAAAATTCTGAAGAA+AGG | 0.568613 | 7.1:+66328532 | MS.gene060423:CDS |
| GGAGAATGTAAAGAGACCAC+TGG | 0.579052 | 7.1:+66332739 | MS.gene060423:CDS |
| GTTTGGTTCCATTATAGCAG+GGG | 0.587823 | 7.1:+66330432 | MS.gene060423:CDS |
| GAAGAAAATTAAGTGGGAAG+CGG | 0.591366 | 7.1:-66332402 | None:intergenic |
| GTGGTTATTAAGACTGAGAG+TGG | 0.592796 | 7.1:-66332707 | None:intergenic |
| TAGCGCTTATGCTGTGACAA+TGG | 0.594696 | 7.1:-66327524 | None:intergenic |
| GAAGCTGATGGAAGAGCTGG+AGG | 0.599267 | 7.1:+66326443 | MS.gene060423:CDS |
| GCTTACAAATTGAAATCACA+TGG | 0.604360 | 7.1:+66326401 | MS.gene060423:CDS |
| CACAGAGGTGGACTCTCAGT+TGG | 0.604737 | 7.1:+66334665 | MS.gene060423:CDS |
| CTCTATACCACCTTCATAGG+GGG | 0.610093 | 7.1:+66332966 | MS.gene060423:CDS |
| TGTTGATGCAGTAATTATGA+CGG | 0.611035 | 7.1:+66330852 | MS.gene060423:CDS |
| ATCAGCTGCTTTGCAACCCG+AGG | 0.614148 | 7.1:-66334699 | None:intergenic |
| GGACGAGGGAGCTAATACAA+TGG | 0.615288 | 7.1:+66326502 | MS.gene060423:CDS |
| GATGGGCTAGTCTGGGACGA+GGG | 0.617395 | 7.1:+66326488 | MS.gene060423:CDS |
| TTTGTAGGTAACCACAGAGG+TGG | 0.618153 | 7.1:+66334653 | MS.gene060423:intron |
| ATGACATACTTGACGAATGT+GGG | 0.618696 | 7.1:-66333365 | None:intergenic |
| GAAAGCTGTTGGGAGCGGAG+GGG | 0.628779 | 7.1:+66333339 | MS.gene060423:CDS |
| CCTCGGGAAGAAAATTAAGT+GGG | 0.629739 | 7.1:-66332408 | None:intergenic |
| AGATGGGCTAGTCTGGGACG+AGG | 0.636611 | 7.1:+66326487 | MS.gene060423:CDS |
| GGTGTCAAGTGGGCTTGCTG+CGG | 0.637668 | 7.1:+66326379 | MS.gene060423:intron |
| GTATAGAGATGCATATCACT+GGG | 0.643232 | 7.1:-66332951 | None:intergenic |
| TCTCTATACCACCTTCATAG+GGG | 0.648326 | 7.1:+66332965 | MS.gene060423:CDS |
| ACACTAGCTTACAGTCATGA+CGG | 0.653321 | 7.1:+66330772 | MS.gene060423:CDS |
| GAAAAGAAAATGAACCACGC+TGG | 0.663833 | 7.1:-66330528 | None:intergenic |
| GAATGTAAAGAGACCACTGG+AGG | 0.665011 | 7.1:+66332742 | MS.gene060423:CDS |
| AGAACTCCAAAACCCTCCAG+TGG | 0.680120 | 7.1:-66332755 | None:intergenic |
| CTCCAACAAAAGGATCAATG+AGG | 0.687378 | 7.1:-66329513 | None:intergenic |
| GATGACATACTTGACGAATG+TGG | 0.697352 | 7.1:-66333366 | None:intergenic |
| AAGCTGATGGAAGAGCTGGA+GGG | 0.698173 | 7.1:+66326444 | MS.gene060423:CDS |
| CAACTGAGAGTCCACCTCTG+TGG | 0.706034 | 7.1:-66334664 | None:intergenic |
| AATGTAAAGAGACCACTGGA+GGG | 0.707965 | 7.1:+66332743 | MS.gene060423:CDS |
| ATGTCTCTGACGTTACCAAG+AGG | 0.711131 | 7.1:-66332358 | None:intergenic |
| AACAATGCTTCAGACAACAG+TGG | 0.735325 | 7.1:+66334740 | MS.gene060423:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 66323656 | 66334769 | 66323656 | ID=MS.gene060423 |
| chr7.1 | mRNA | 66323656 | 66334769 | 66323656 | ID=MS.gene060423.t1;Parent=MS.gene060423 |
| chr7.1 | exon | 66323656 | 66323686 | 66323656 | ID=MS.gene060423.t1.exon1;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66323656 | 66323686 | 66323656 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66323848 | 66323887 | 66323848 | ID=MS.gene060423.t1.exon2;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66323848 | 66323887 | 66323848 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66326388 | 66326523 | 66326388 | ID=MS.gene060423.t1.exon3;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66326388 | 66326523 | 66326388 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66327201 | 66327266 | 66327201 | ID=MS.gene060423.t1.exon4;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66327201 | 66327266 | 66327201 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66327524 | 66327574 | 66327524 | ID=MS.gene060423.t1.exon5;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66327524 | 66327574 | 66327524 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66327717 | 66327773 | 66327717 | ID=MS.gene060423.t1.exon6;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66327717 | 66327773 | 66327717 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66328477 | 66328553 | 66328477 | ID=MS.gene060423.t1.exon7;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66328477 | 66328553 | 66328477 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66328668 | 66328704 | 66328668 | ID=MS.gene060423.t1.exon8;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66328668 | 66328704 | 66328668 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66329502 | 66329567 | 66329502 | ID=MS.gene060423.t1.exon9;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66329502 | 66329567 | 66329502 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66329690 | 66329730 | 66329690 | ID=MS.gene060423.t1.exon10;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66329690 | 66329730 | 66329690 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66330432 | 66330564 | 66330432 | ID=MS.gene060423.t1.exon11;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66330432 | 66330564 | 66330432 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66330706 | 66330873 | 66330706 | ID=MS.gene060423.t1.exon12;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66330706 | 66330873 | 66330706 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66332355 | 66332429 | 66332355 | ID=MS.gene060423.t1.exon13;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66332355 | 66332429 | 66332355 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66332695 | 66332818 | 66332695 | ID=MS.gene060423.t1.exon14;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66332695 | 66332818 | 66332695 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66332913 | 66333021 | 66332913 | ID=MS.gene060423.t1.exon15;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66332913 | 66333021 | 66332913 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66333304 | 66333378 | 66333304 | ID=MS.gene060423.t1.exon16;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66333304 | 66333378 | 66333304 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66333444 | 66333550 | 66333444 | ID=MS.gene060423.t1.exon17;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66333444 | 66333550 | 66333444 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| chr7.1 | exon | 66334660 | 66334769 | 66334660 | ID=MS.gene060423.t1.exon18;Parent=MS.gene060423.t1 |
| chr7.1 | CDS | 66334660 | 66334769 | 66334660 | ID=cds.MS.gene060423.t1;Parent=MS.gene060423.t1 |
| Gene Sequence |
| Protein sequence |
