Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060510.t1 | AET01987.1 | 68.8 | 205 | 16 | 1 | 36 | 192 | 37 | 241 | 5.80E-62 | 247.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060510.t1 | Q9M276 | 36.1 | 205 | 83 | 6 | 35 | 192 | 32 | 235 | 9.2e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060510.t1 | G7LAS8 | 68.8 | 205 | 16 | 1 | 36 | 192 | 37 | 241 | 4.2e-62 | 247.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060510.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene060510 | MS.gene84836 | PPI |
| MS.gene060510 | MS.gene067308 | PPI |
| MS.gene060510 | MS.gene84723 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060510.t1 | MTR_8g026960 | 76.763 | 241 | 7 | 2 | 1 | 192 | 1 | 241 | 1.05e-125 | 354 |
| MS.gene060510.t1 | MTR_3g092150 | 32.039 | 206 | 92 | 5 | 35 | 192 | 40 | 245 | 6.04e-17 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060510.t1 | AT2G46680 | 36.719 | 256 | 88 | 8 | 11 | 192 | 3 | 258 | 6.97e-29 | 108 |
| MS.gene060510.t1 | AT2G46680 | 37.647 | 255 | 85 | 9 | 11 | 192 | 3 | 256 | 1.39e-28 | 107 |
| MS.gene060510.t1 | AT3G61890 | 37.915 | 211 | 69 | 7 | 36 | 192 | 33 | 235 | 4.22e-26 | 100 |
Find 24 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACTCTGACTTTGCTCTAT+TGG | 0.221851 | 8.1:+75427184 | None:intergenic |
| GCTAACTGCAACTTCTTTCT+TGG | 0.243093 | 8.1:+75427750 | None:intergenic |
| ATTATCAATGGTGGGACTTT+TGG | 0.297638 | 8.1:-75426883 | MS.gene060510:CDS |
| TTTGAATCAAATGATTTGTT+AGG | 0.319546 | 8.1:-75426920 | MS.gene060510:CDS |
| TCAAAAGCACTCCAATCTTC+TGG | 0.368233 | 8.1:+75426938 | None:intergenic |
| TTTGGTTTAAAAGATGAAAC+TGG | 0.375939 | 8.1:-75427004 | MS.gene060510:CDS |
| TGTTCTGATCTTTCCATTGA+AGG | 0.384421 | 8.1:+75427076 | None:intergenic |
| CTTTAACATCACCAGAAGAT+TGG | 0.390865 | 8.1:-75426949 | MS.gene060510:CDS |
| ATGGAAAGTGCATCAGAAAA+TGG | 0.406512 | 8.1:-75427136 | MS.gene060510:CDS |
| TGATGAACAAATCAAATCAT+TGG | 0.409448 | 8.1:-75427805 | MS.gene060510:CDS |
| AGCATAAAGGTTGAATACTT+TGG | 0.411821 | 8.1:-75427022 | MS.gene060510:CDS |
| GATCTTTCCATTGAAGGACT+TGG | 0.420894 | 8.1:+75427082 | None:intergenic |
| TTGCAGTTAGCTAGAGAGCT+TGG | 0.431832 | 8.1:-75427738 | MS.gene060510:CDS |
| AGATGATGACACAAGCATAA+AGG | 0.461800 | 8.1:-75427035 | MS.gene060510:CDS |
| AACCATATAGCAACTTGTCT+TGG | 0.480304 | 8.1:+75427708 | None:intergenic |
| GGTGAAACCAAGTCCTTCAA+TGG | 0.505148 | 8.1:-75427089 | MS.gene060510:CDS |
| TATAGTTACTCAGCAGAAGC+AGG | 0.509566 | 8.1:-75427903 | MS.gene060510:CDS |
| AATCAAGTTGTGATTATCAA+TGG | 0.520398 | 8.1:-75426895 | MS.gene060510:CDS |
| CAAGTTGTGATTATCAATGG+TGG | 0.557189 | 8.1:-75426892 | MS.gene060510:CDS |
| AACTATGAAATGTGAGGCTG+AGG | 0.573068 | 8.1:-75427110 | MS.gene060510:CDS |
| AAGTTGTGATTATCAATGGT+GGG | 0.604345 | 8.1:-75426891 | MS.gene060510:CDS |
| AGTTAAAGAAGCAAACAGCA+TGG | 0.635070 | 8.1:-75427155 | MS.gene060510:CDS |
| GAAAGTGCATCAGAAAATGG+AGG | 0.672573 | 8.1:-75427133 | MS.gene060510:CDS |
| TGGAGGAACTATGAAATGTG+AGG | 0.703839 | 8.1:-75427116 | MS.gene060510:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAAATAGTTACAATAATT+TGG | - | chr8.1:75427167-75427186 | MS.gene060510:CDS | 15.0% |
| !! | TGCGAAATTATTATTAATAA+CGG | - | chr8.1:75427367-75427386 | MS.gene060510:intron | 15.0% |
| !! | GAAATTATTATTAATAACGG+TGG | - | chr8.1:75427370-75427389 | MS.gene060510:intron | 20.0% |
| !! | TAATCATATAGTAATTTGAC+TGG | - | chr8.1:75427542-75427561 | MS.gene060510:intron | 20.0% |
| !!! | ATAAATTTGTTTGCTTAGAA+TGG | - | chr8.1:75427275-75427294 | MS.gene060510:intron | 20.0% |
| !!! | TTTGAATCAAATGATTTGTT+AGG | - | chr8.1:75427875-75427894 | MS.gene060510:CDS | 20.0% |
| ! | AAGACAAACATTACTAATAC+AGG | - | chr8.1:75427218-75427237 | MS.gene060510:intron | 25.0% |
| ! | AGAAAAACAAGAATACAAAG+AGG | - | chr8.1:75426962-75426981 | MS.gene060510:CDS | 25.0% |
| ! | ATCTTGTCAACATTTCAAAA+TGG | + | chr8.1:75427343-75427362 | None:intergenic | 25.0% |
| ! | TGATGAACAAATCAAATCAT+TGG | - | chr8.1:75426990-75427009 | MS.gene060510:CDS | 25.0% |
| !! | AATCAAGTTGTGATTATCAA+TGG | - | chr8.1:75427900-75427919 | MS.gene060510:CDS | 25.0% |
| !! | TTTGGTTTAAAAGATGAAAC+TGG | - | chr8.1:75427791-75427810 | MS.gene060510:CDS | 25.0% |
| AAAGTTTGAATCTATGACGA+AGG | - | chr8.1:75427194-75427213 | MS.gene060510:CDS | 30.0% | |
| AAGTTGTGATTATCAATGGT+GGG | - | chr8.1:75427904-75427923 | MS.gene060510:CDS | 30.0% | |
| AGCATAAAGGTTGAATACTT+TGG | - | chr8.1:75427773-75427792 | MS.gene060510:CDS | 30.0% | |
| TTCAAAACAAAAGAGCTAGA+TGG | - | chr8.1:75427109-75427128 | MS.gene060510:CDS | 30.0% | |
| !! | CTTGTTTTTCTTCTTTCTCA+AGG | + | chr8.1:75426953-75426972 | None:intergenic | 30.0% |
| AACCATATAGCAACTTGTCT+TGG | + | chr8.1:75427090-75427109 | None:intergenic | 35.0% | |
| AGATGATGACACAAGCATAA+AGG | - | chr8.1:75427760-75427779 | MS.gene060510:CDS | 35.0% | |
| AGTTAAAGAAGCAAACAGCA+TGG | - | chr8.1:75427640-75427659 | MS.gene060510:intron | 35.0% | |
| CAAGTTGTGATTATCAATGG+TGG | - | chr8.1:75427903-75427922 | MS.gene060510:CDS | 35.0% | |
| CTAATAACCATCGTGTTCAT+CGG | + | chr8.1:75427469-75427488 | None:intergenic | 35.0% | |
| CTTTAACATCACCAGAAGAT+TGG | - | chr8.1:75427846-75427865 | MS.gene060510:CDS | 35.0% | |
| GATGAACACGATGGTTATTA+GGG | - | chr8.1:75427468-75427487 | MS.gene060510:intron | 35.0% | |
| TGTTCTGATCTTTCCATTGA+AGG | + | chr8.1:75427722-75427741 | None:intergenic | 35.0% | |
| ! | AATTTTGCTGAACATGCTGA+TGG | - | chr8.1:75427821-75427840 | MS.gene060510:CDS | 35.0% |
| ! | ATGGAAAGTGCATCAGAAAA+TGG | - | chr8.1:75427659-75427678 | MS.gene060510:intron | 35.0% |
| ! | GATTGACTTTGTATCTTCAG+TGG | + | chr8.1:75427427-75427446 | None:intergenic | 35.0% |
| !! | ATTATCAATGGTGGGACTTT+TGG | - | chr8.1:75427912-75427931 | MS.gene060510:CDS | 35.0% |
| AACTATGAAATGTGAGGCTG+AGG | - | chr8.1:75427685-75427704 | MS.gene060510:intron | 40.0% | |
| AGCCAAGACAAGTTGCTATA+TGG | - | chr8.1:75427085-75427104 | MS.gene060510:CDS | 40.0% | |
| CGATGAACACGATGGTTATT+AGG | - | chr8.1:75427467-75427486 | MS.gene060510:intron | 40.0% | |
| CTACTCTGACTTTGCTCTAT+TGG | + | chr8.1:75427614-75427633 | None:intergenic | 40.0% | |
| GATCTTTCCATTGAAGGACT+TGG | + | chr8.1:75427716-75427735 | None:intergenic | 40.0% | |
| GCTAACTGCAACTTCTTTCT+TGG | + | chr8.1:75427048-75427067 | None:intergenic | 40.0% | |
| TATAGTTACTCAGCAGAAGC+AGG | - | chr8.1:75426892-75426911 | MS.gene060510:CDS | 40.0% | |
| TGGAGGAACTATGAAATGTG+AGG | - | chr8.1:75427679-75427698 | MS.gene060510:intron | 40.0% | |
| ! | GAAAGTGCATCAGAAAATGG+AGG | - | chr8.1:75427662-75427681 | MS.gene060510:intron | 40.0% |
| !! | TCAAAAGCACTCCAATCTTC+TGG | + | chr8.1:75427860-75427879 | None:intergenic | 40.0% |
| GGTGAAACCAAGTCCTTCAA+TGG | - | chr8.1:75427706-75427725 | MS.gene060510:intron | 45.0% | |
| GTTCATCGGTATCAACTCAG+TGG | + | chr8.1:75427455-75427474 | None:intergenic | 45.0% | |
| GTTGATACCGATGAACACGA+TGG | - | chr8.1:75427459-75427478 | MS.gene060510:intron | 45.0% | |
| TTGCAGTTAGCTAGAGAGCT+TGG | - | chr8.1:75427057-75427076 | MS.gene060510:CDS | 45.0% | |
| !! | ATTAAAAATTAAAATTGAAT+TGG | + | chr8.1:75427320-75427339 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 75426877 | 75427940 | 75426877 | ID=MS.gene060510 |
| chr8.1 | mRNA | 75426877 | 75427940 | 75426877 | ID=MS.gene060510.t1;Parent=MS.gene060510 |
| chr8.1 | exon | 75427722 | 75427940 | 75427722 | ID=MS.gene060510.t1.exon1;Parent=MS.gene060510.t1 |
| chr8.1 | CDS | 75427722 | 75427940 | 75427722 | ID=cds.MS.gene060510.t1;Parent=MS.gene060510.t1 |
| chr8.1 | exon | 75426877 | 75427236 | 75426877 | ID=MS.gene060510.t1.exon2;Parent=MS.gene060510.t1 |
| chr8.1 | CDS | 75426877 | 75427236 | 75426877 | ID=cds.MS.gene060510.t1;Parent=MS.gene060510.t1 |
| Gene Sequence |
| Protein sequence |
