AlfalfaGEDB | MS.gene067308

M. sativa cultivar XinJiangDaYe / MS.gene067308

KEGG: K09338
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32 PF02183.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067308.t1	AFV30231.1	97.1	240	6	1	1	239	8	247	9.80E-75	290

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067308.t1	Q9M276	64.0	211	64	5	34	239	32	235	4.5e-28	126.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067308.t1	K4MNF8	97.1	240	6	1	1	239	8	247	7.0e-75	290.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene067308.t1	TF	HB-HD-ZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049983	MS.gene067308	0.82009	8.24E-53	-1.69E-46
MS.gene050384	MS.gene067308	0.840467	8.54E-58	-1.69E-46
MS.gene050386	MS.gene067308	0.836327	9.98E-57	-1.69E-46
MS.gene051856	MS.gene067308	0.802493	5.46E-49	-1.69E-46
MS.gene052954	MS.gene067308	0.808741	2.67E-50	-1.69E-46
MS.gene053188	MS.gene067308	0.810736	9.94E-51	-1.69E-46
MS.gene053575	MS.gene067308	0.807229	5.60E-50	-1.69E-46
MS.gene053931	MS.gene067308	0.850163	2.03E-60	-1.69E-46
MS.gene05686	MS.gene067308	0.802856	4.60E-49	-1.69E-46
MS.gene057392	MS.gene067308	0.833303	5.76E-56	-1.69E-46
MS.gene058008	MS.gene067308	0.815739	7.92E-52	-1.69E-46
MS.gene058009	MS.gene067308	0.8128	3.53E-51	-1.69E-46
MS.gene059867	MS.gene067308	0.810227	1.28E-50	-1.69E-46
MS.gene060935	MS.gene067308	0.815776	7.77E-52	-1.69E-46
MS.gene060937	MS.gene067308	0.804035	2.62E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene067308	MS.gene84723	PPI
MS.gene060510	MS.gene067308	PPI
MS.gene067308	MS.gene84836	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067308.t1	MTR_8g026960	96.266	241	7	2	1	239	1	241	2.33e-170	469
MS.gene067308.t1	MTR_3g092150	43.062	209	113	3	34	239	40	245	6.75e-48	158
MS.gene067308.t1	MTR_6g007647	53.125	96	45	0	34	129	35	130	4.23e-30	112
MS.gene067308.t1	MTR_6g007647	53.125	96	45	0	34	129	69	164	1.09e-29	112
MS.gene067308.t1	MTR_8g089895	48.148	108	56	0	34	141	56	163	4.12e-29	111
MS.gene067308.t1	MTR_6g011610	51.961	102	45	1	23	124	72	169	1.60e-26	103
MS.gene067308.t1	MTR_6g011610	53.846	91	42	0	34	124	98	188	1.65e-26	103
MS.gene067308.t1	MTR_8g468210	41.270	126	70	1	1	126	50	171	2.81e-26	103
MS.gene067308.t1	MTR_5g038280	41.176	136	73	2	3	137	64	193	4.42e-26	103
MS.gene067308.t1	MTR_3g086790	40.127	157	85	5	34	186	62	213	5.79e-26	103
MS.gene067308.t1	MTR_8g469430	37.222	180	90	6	34	196	91	264	3.02e-25	100
MS.gene067308.t1	MTR_1g061660	36.628	172	82	6	3	159	24	183	5.45e-25	99.0
MS.gene067308.t1	MTR_4g107650	42.478	113	62	1	34	146	97	206	7.69e-25	100
MS.gene067308.t1	MTR_3g080100	47.253	91	48	0	34	124	22	112	8.36e-24	95.5
MS.gene067308.t1	MTR_3g080100	47.253	91	48	0	34	124	61	151	1.29e-23	95.9
MS.gene067308.t1	MTR_5g039000	40.984	122	71	1	6	126	50	171	2.51e-23	95.5
MS.gene067308.t1	MTR_7g103340	48.352	91	40	1	34	124	65	148	6.27e-22	90.9
MS.gene067308.t1	MTR_7g010020	41.129	124	62	2	1	124	53	165	1.80e-21	90.5
MS.gene067308.t1	MTR_5g019680	51.852	81	34	1	20	100	46	121	3.12e-18	80.5
MS.gene067308.t1	MTR_5g019650	51.852	81	34	1	20	100	46	121	1.10e-17	79.3
MS.gene067308.t1	MTR_4g100550	44.578	83	46	0	35	117	123	205	3.24e-14	70.5
MS.gene067308.t1	MTR_7g093430	45.783	83	45	0	35	117	163	245	5.85e-14	70.1
MS.gene067308.t1	MTR_4g126900	36.975	119	67	2	35	152	91	202	8.77e-14	68.6
MS.gene067308.t1	MTR_5g014890	35.200	125	70	3	35	155	126	243	1.88e-12	65.5
MS.gene067308.t1	MTR_7g027265	43.243	74	39	1	15	88	6	76	2.95e-12	62.8
MS.gene067308.t1	MTR_1g054285	43.373	83	47	0	35	117	157	239	3.84e-12	65.1
MS.gene067308.t1	MTR_5g013010	42.169	83	48	0	35	117	139	221	8.31e-12	63.9
MS.gene067308.t1	MTR_2g061030	41.111	90	50	1	31	117	196	285	1.22e-11	63.9
MS.gene067308.t1	MTR_4g097600	42.169	83	48	0	35	117	130	212	1.95e-11	62.8
MS.gene067308.t1	MTR_3g103590	31.707	123	80	2	35	154	148	269	2.79e-11	62.4
MS.gene067308.t1	MTR_8g006705	42.169	83	48	0	35	117	149	231	3.63e-11	62.0
MS.gene067308.t1	MTR_1g017090	40.964	83	49	0	35	117	133	215	6.80e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067308.t1	AT2G46680	49.225	258	109	7	2	239	3	258	2.84e-65	203
MS.gene067308.t1	AT3G61890	55.238	210	82	5	35	239	33	235	3.21e-63	197
MS.gene067308.t1	AT2G46680	48.450	258	109	8	2	239	3	256	8.71e-62	194
MS.gene067308.t1	AT3G01470	44.531	128	70	1	34	160	70	197	4.96e-31	115
MS.gene067308.t1	AT2G22430	52.747	91	43	0	34	124	64	154	1.30e-28	110
MS.gene067308.t1	AT4G40060	50.549	91	45	0	34	124	61	151	2.65e-28	109
MS.gene067308.t1	AT5G65310	48.113	106	49	1	19	124	47	146	1.47e-27	107
MS.gene067308.t1	AT5G65310	48.113	106	49	1	19	124	65	164	2.20e-27	107
MS.gene067308.t1	AT1G69780	46.429	112	55	2	13	124	71	177	5.01e-26	103
MS.gene067308.t1	AT5G15150	40.000	145	77	3	11	154	96	231	8.30e-26	102
MS.gene067308.t1	AT3G01220	45.946	111	50	2	14	124	72	172	1.71e-24	99.0
MS.gene067308.t1	AT4G36740	57.746	71	30	0	34	104	57	127	1.55e-22	92.0
MS.gene067308.t1	AT4G36740	57.746	71	30	0	34	104	58	128	1.67e-22	92.0
MS.gene067308.t1	AT1G26960	48.214	112	46	3	13	124	57	156	1.81e-22	92.8
MS.gene067308.t1	AT2G18550	42.500	120	61	2	34	145	63	182	2.10e-21	89.4
MS.gene067308.t1	AT5G03790	44.792	96	46	1	34	129	79	167	3.45e-21	89.0
MS.gene067308.t1	AT1G27045	37.681	138	77	1	7	135	34	171	1.22e-20	87.8
MS.gene067308.t1	AT5G03790	45.263	95	52	0	34	128	79	173	1.76e-20	86.3
MS.gene067308.t1	AT5G66700	53.521	71	33	0	34	104	73	143	3.08e-20	86.3
MS.gene067308.t1	AT1G27045	48.810	84	43	0	34	117	27	110	3.17e-19	82.4
MS.gene067308.t1	AT1G27045	48.810	84	43	0	34	117	27	110	3.17e-19	82.4
MS.gene067308.t1	AT1G27045	48.810	84	43	0	34	117	19	102	3.20e-19	82.4
MS.gene067308.t1	AT1G27045	39.167	120	64	1	7	117	34	153	3.54e-19	83.6
MS.gene067308.t1	AT2G36610	41.176	102	48	2	31	124	70	167	4.41e-18	79.3
MS.gene067308.t1	AT5G53980	41.071	112	58	2	23	134	3	106	1.06e-16	75.1
MS.gene067308.t1	AT3G60390	35.398	113	68	1	1	113	136	243	1.06e-11	63.9
MS.gene067308.t1	AT4G37790	40.964	83	49	0	35	117	129	211	1.25e-11	63.5
MS.gene067308.t1	AT2G22800	42.169	83	48	0	35	117	116	198	1.40e-11	63.2
MS.gene067308.t1	AT2G44910	41.772	79	46	0	35	113	166	244	1.69e-11	63.2
MS.gene067308.t1	AT5G06710	39.759	83	50	0	35	117	193	275	2.32e-11	63.2
MS.gene067308.t1	AT4G17460	41.772	79	46	0	35	113	138	216	4.57e-11	61.6
MS.gene067308.t1	AT4G16780	41.772	79	46	0	35	113	132	210	5.56e-11	61.6

Find 26 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAAAATAGTTACAATAATT+TGG	0.124744	8.2:-71059689	MS.gene067308:CDS
CTACTCTGACTTTGCTCTAT+TGG	0.221851	8.2:+71059245	None:intergenic
GCTAATTGCAACTTCTTTCT+TGG	0.222692	8.2:+71059811	None:intergenic
ATTATCAATGGTGGGACTTT+TGG	0.297638	8.2:-71058944	MS.gene067308:CDS
TTTGAATCAAATGATTTGTT+AGG	0.319546	8.2:-71058981	MS.gene067308:CDS
AGCCAAGACAAGTTGCTATA+TGG	0.331941	8.2:-71059771	MS.gene067308:CDS
TCAAAAGCACTCCAATCTTC+TGG	0.368233	8.2:+71058999	None:intergenic
TGTTCTGATCTTTCTATTGA+AGG	0.372040	8.2:+71059137	None:intergenic
ATGGAAAGTGCAGCAGAAAA+TGG	0.377944	8.2:-71059197	MS.gene067308:CDS
CTTTAACATCACCAGAAGAT+TGG	0.390865	8.2:-71059010	MS.gene067308:CDS
TGATGAACAAATCAAATCAT+TGG	0.409448	8.2:-71059866	MS.gene067308:CDS
TTTGGTTTAGAAGATGAAAC+TGG	0.409611	8.2:-71059065	MS.gene067308:CDS
AGCATAAAGGTTGAATACTT+TGG	0.411821	8.2:-71059083	MS.gene067308:CDS
TTCAAAACAAAAGAGCTAGA+TGG	0.440130	8.2:-71059747	MS.gene067308:CDS
TTGCAATTAGCTAGAGAGCT+TGG	0.453561	8.2:-71059799	MS.gene067308:CDS
AAAGTTTGAATCTATGAAGA+AGG	0.456244	8.2:-71059662	MS.gene067308:CDS
AGATGATGACACAAGCATAA+AGG	0.461800	8.2:-71059096	MS.gene067308:CDS
AAGACAAACATTACTAATAC+AGG	0.463639	8.2:-71059638	MS.gene067308:intron
AACCATATAGCAACTTGTCT+TGG	0.480304	8.2:+71059769	None:intergenic
GATCTTTCTATTGAAGGACT+TGG	0.483251	8.2:+71059143	None:intergenic
TATAGTTATTCAGCAGAAGC+AGG	0.509566	8.2:-71059961	MS.gene067308:CDS
AATCAAGTTGTGATTATCAA+TGG	0.520398	8.2:-71058956	MS.gene067308:CDS
CAAGTTGTGATTATCAATGG+TGG	0.557189	8.2:-71058953	MS.gene067308:CDS
AAGTTGTGATTATCAATGGT+GGG	0.604345	8.2:-71058952	MS.gene067308:CDS
AGTTAAAGAAGCAAACAGCA+TGG	0.635070	8.2:-71059216	MS.gene067308:CDS
GAAAGTGCAGCAGAAAATGG+AGG	0.672573	8.2:-71059194	MS.gene067308:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCAAAATAGTTACAATAATT+TGG	-	chr8.2:71059225-71059244	MS.gene067308:CDS	15.0%
!!	TGCGAAATTATTATTAATAA+CGG	-	chr8.2:71059425-71059444	MS.gene067308:intron	15.0%
!!	GAAATTATTATTAATAACGG+TGG	-	chr8.2:71059428-71059447	MS.gene067308:intron	20.0%
!!	TAATCATATAGTAATTTGAC+TGG	-	chr8.2:71059600-71059619	MS.gene067308:intron	20.0%
!!!	ATAAATTTGTTTGCTTAGAA+TGG	-	chr8.2:71059333-71059352	MS.gene067308:intron	20.0%
!!!	TTTGAATCAAATGATTTGTT+AGG	-	chr8.2:71059933-71059952	MS.gene067308:CDS	20.0%
!	AAAGTTTGAATCTATGAAGA+AGG	-	chr8.2:71059252-71059271	MS.gene067308:CDS	25.0%
!	AAGACAAACATTACTAATAC+AGG	-	chr8.2:71059276-71059295	MS.gene067308:CDS	25.0%
!	AGAAAAACAAGAATACAAAG+AGG	-	chr8.2:71059020-71059039	MS.gene067308:CDS	25.0%
!	ATCTTGTCAACATTTCAAAA+TGG	+	chr8.2:71059401-71059420	None:intergenic	25.0%
!	TGATGAACAAATCAAATCAT+TGG	-	chr8.2:71059048-71059067	MS.gene067308:CDS	25.0%
!!	AATCAAGTTGTGATTATCAA+TGG	-	chr8.2:71059958-71059977	MS.gene067308:CDS	25.0%
	AAGTTGTGATTATCAATGGT+GGG	-	chr8.2:71059962-71059981	MS.gene067308:CDS	30.0%
	AGCATAAAGGTTGAATACTT+TGG	-	chr8.2:71059831-71059850	MS.gene067308:CDS	30.0%
	TGTTCTGATCTTTCTATTGA+AGG	+	chr8.2:71059780-71059799	None:intergenic	30.0%
	TTCAAAACAAAAGAGCTAGA+TGG	-	chr8.2:71059167-71059186	MS.gene067308:CDS	30.0%
!	TTTGGTTTAGAAGATGAAAC+TGG	-	chr8.2:71059849-71059868	MS.gene067308:CDS	30.0%
!!	CTTGTTTTTCTTCTTTCTCA+TGG	+	chr8.2:71059011-71059030	None:intergenic	30.0%
	AAAAATCAAATGTGAGGCTG+AGG	-	chr8.2:71059743-71059762	MS.gene067308:CDS	35.0%
	AACCATATAGCAACTTGTCT+TGG	+	chr8.2:71059148-71059167	None:intergenic	35.0%
	AGATGATGACACAAGCATAA+AGG	-	chr8.2:71059818-71059837	MS.gene067308:CDS	35.0%
	AGTTAAAGAAGCAAACAGCA+TGG	-	chr8.2:71059698-71059717	MS.gene067308:CDS	35.0%
	CAAGTTGTGATTATCAATGG+TGG	-	chr8.2:71059961-71059980	MS.gene067308:CDS	35.0%
	CTAATAACCATCGTGTTCAT+CGG	+	chr8.2:71059527-71059546	None:intergenic	35.0%
	CTTTAACATCACCAGAAGAT+TGG	-	chr8.2:71059904-71059923	MS.gene067308:CDS	35.0%
	GATCTTTCTATTGAAGGACT+TGG	+	chr8.2:71059774-71059793	None:intergenic	35.0%
	GATGAACACGATGGTTATTA+GGG	-	chr8.2:71059526-71059545	MS.gene067308:intron	35.0%
	GCTAATTGCAACTTCTTTCT+TGG	+	chr8.2:71059106-71059125	None:intergenic	35.0%
	TATAGTTATTCAGCAGAAGC+AGG	-	chr8.2:71058953-71058972	MS.gene067308:CDS	35.0%
	TGGAGGAAAAATCAAATGTG+AGG	-	chr8.2:71059737-71059756	MS.gene067308:CDS	35.0%
!	AATTTTGCTGAACATGCTGA+TGG	-	chr8.2:71059879-71059898	MS.gene067308:CDS	35.0%
!	GATTGACTTTGTATCTTCAG+TGG	+	chr8.2:71059485-71059504	None:intergenic	35.0%
!!	ATTATCAATGGTGGGACTTT+TGG	-	chr8.2:71059970-71059989	MS.gene067308:CDS	35.0%
	AGCCAAGACAAGTTGCTATA+TGG	-	chr8.2:71059143-71059162	MS.gene067308:CDS	40.0%
	CGATGAACACGATGGTTATT+AGG	-	chr8.2:71059525-71059544	MS.gene067308:intron	40.0%
	CTACTCTGACTTTGCTCTAT+TGG	+	chr8.2:71059672-71059691	None:intergenic	40.0%
	TTGCAATTAGCTAGAGAGCT+TGG	-	chr8.2:71059115-71059134	MS.gene067308:CDS	40.0%
!	ATGGAAAGTGCAGCAGAAAA+TGG	-	chr8.2:71059717-71059736	MS.gene067308:CDS	40.0%
!!	TCAAAAGCACTCCAATCTTC+TGG	+	chr8.2:71059918-71059937	None:intergenic	40.0%
	GTTCATCGGTATCAACTCAG+TGG	+	chr8.2:71059513-71059532	None:intergenic	45.0%
	GTTGATACCGATGAACACGA+TGG	-	chr8.2:71059517-71059536	MS.gene067308:intron	45.0%
!	GAAAGTGCAGCAGAAAATGG+AGG	-	chr8.2:71059720-71059739	MS.gene067308:CDS	45.0%
!!	ATTAAAAATTAAAATTGAAT+TGG	+	chr8.2:71059378-71059397	None:intergenic	5.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	71058938	71059998	71058938	ID=MS.gene067308
chr8.2	mRNA	71058938	71059998	71058938	ID=MS.gene067308.t1;Parent=MS.gene067308
chr8.2	exon	71059639	71059998	71059639	ID=MS.gene067308.t1.exon1;Parent=MS.gene067308.t1
chr8.2	CDS	71059639	71059998	71059639	ID=cds.MS.gene067308.t1;Parent=MS.gene067308.t1
chr8.2	exon	71058938	71059297	71058938	ID=MS.gene067308.t1.exon2;Parent=MS.gene067308.t1
chr8.2	CDS	71058938	71059297	71058938	ID=cds.MS.gene067308.t1;Parent=MS.gene067308.t1

Gene Sequence

>MS.gene067308

ATGGAATATAGTCAATATAGTTATTCAGCAGAAGCAGGAGAAGAAACATACACAACTAGTAGCACATCTTCCATGAGAAAGAAGAAAAACAAGAATACAAAGAGGTTTAGTGATGAACAAATCAAATCATTGGAAACTATGTTTGAGACTGAGACAAGACTTGAACCAAGAAAGAAGTTGCAATTAGCTAGAGAGCTTGGATTGCAGCCAAGACAAGTTGCTATATGGTTTCAAAACAAAAGAGCTAGATGGAAATCAAAGCAACTTGAAAGAGAATACAACAAACTTCAAAATAGTTACAATAATTTGGCTTCAAAGTTTGAATCTATGAAGAAGGAAAGACAAACATTACTAATACAGGTACTTAGTAGTTGCTAATTAGTAATAATTTTCATATAAATTTGTTTGCTTAGAATGGACTAACATATGTCATATATCCAATTCAATTTTAATTTTTAATCCATTTTGAAATGTTGACAAGATATCGTGCGAAATTATTATTAATAACGGTGGCACTAATAGTTATTTTACAATTGTCACATGTCCACTGAAGATACAAAGTCAATCTCTTACCACTGAGTTGATACCGATGAACACGATGGTTATTAGGGTCACTTAAATTACTAATATATTACATTTTGTATTAATTATTATCATATAGTTAATCATATAGTAATTTGACTGGTTTTCATGTTCAACAGTTGCAGAAGCTGAATGATCTAATACAAAAGCCAATAGAGCAAAGTCAGAGTAGTTCACAAGTTAAAGAAGCAAACAGCATGGAAAGTGCAGCAGAAAATGGAGGAAAAATCAAATGTGAGGCTGAGGTGAAACCAAGTCCTTCAATAGAAAGATCAGAACATGTACTTGATGTTCTATCAGATGATGACACAAGCATAAAGGTTGAATACTTTGGTTTAGAAGATGAAACTGGTCTTATGAATTTTGCTGAACATGCTGATGGTTCTTTAACATCACCAGAAGATTGGAGTGCTTTTGAATCAAATGATTTGTTAGGTCAATCAAGTTGTGATTATCAATGGTGGGACTTTTGGTCTTGA

Protein sequence

>MS.gene067308.t1

MEYSQYSYSAEAGEETYTTSSTSSMRKKKNKNTKRFSDEQIKSLETMFETETRLEPRKKLQLARELGLQPRQVAIWFQNKRARWKSKQLEREYNKLQNSYNNLASKFESMKKERQTLLIQLQKLNDLIQKPIEQSQSSSQVKEANSMESAAENGGKIKCEAEVKPSPSIERSEHVLDVLSDDDTSIKVEYFGLEDETGLMNFAEHADGSLTSPEDWSAFESNDLLGQSSCDYQWWDFWS