AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06058

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06058.t1 MTR_8g099320 97.902 429 9 0 23 451 25 453 0.0 855
MS.gene06058.t1 MTR_4g055610 49.173 423 214 1 23 445 38 459 1.07e-147 429
MS.gene06058.t1 MTR_1g492640 46.224 437 225 4 20 448 47 481 5.56e-133 392
MS.gene06058.t1 MTR_1g492640 44.729 351 191 3 99 448 1 349 1.88e-100 304
MS.gene06058.t1 MTR_1g492640 46.562 320 161 4 20 331 47 364 3.05e-93 287
MS.gene06058.t1 MTR_1g492640 46.562 320 161 4 20 331 29 346 3.10e-93 286
MS.gene06058.t1 MTR_1g070470 32.710 428 271 6 24 445 21 437 3.38e-70 229
MS.gene06058.t1 MTR_4g033275 97.826 92 2 0 323 414 7 98 3.49e-59 189
MS.gene06058.t1 MTR_6g022790 28.477 453 298 10 5 446 18 455 6.76e-56 192
MS.gene06058.t1 MTR_4g055637 46.535 202 100 3 244 445 182 375 6.22e-55 187
MS.gene06058.t1 MTR_2g101920 28.472 432 288 8 21 445 43 460 4.17e-52 182
MS.gene06058.t1 MTR_1g492640 46.907 194 94 3 20 205 29 221 9.50e-49 167
MS.gene06058.t1 MTR_1g492640 46.907 194 94 3 20 205 47 239 1.12e-48 168
MS.gene06058.t1 MTR_7g038910 28.904 429 283 9 25 446 30 443 9.37e-48 170
MS.gene06058.t1 MTR_6g022780 28.868 433 287 8 21 446 10 428 6.91e-47 167
MS.gene06058.t1 MTR_6g023075 27.854 438 295 8 16 446 5 428 2.86e-45 163
MS.gene06058.t1 MTR_8g080120 26.545 437 291 9 21 446 19 436 3.09e-45 163
MS.gene06058.t1 MTR_7g038910 29.513 349 224 9 25 366 30 363 6.32e-43 155
MS.gene06058.t1 MTR_2g096830 28.176 433 290 8 21 446 10 428 5.06e-42 154
MS.gene06058.t1 MTR_7g038910 29.446 343 220 9 25 360 30 357 5.45e-42 153
MS.gene06058.t1 MTR_6g023065 27.416 445 297 11 13 446 34 463 6.51e-42 155
MS.gene06058.t1 MTR_4g104510 28.894 398 252 12 56 445 71 445 4.33e-40 149
MS.gene06058.t1 MTR_3g069960 29.348 368 232 10 87 445 32 380 9.91e-40 147
MS.gene06058.t1 MTR_4g104510 28.750 400 252 12 56 445 71 447 1.09e-39 148
MS.gene06058.t1 MTR_4g103890 26.977 430 286 9 25 444 10 421 1.64e-39 147
MS.gene06058.t1 MTR_3g069960 29.348 368 232 10 87 445 85 433 2.79e-39 147
MS.gene06058.t1 MTR_5g081630 28.750 400 252 11 56 445 4 380 3.98e-39 145
MS.gene06058.t1 MTR_3g005740 27.191 445 288 13 18 446 45 469 1.03e-38 146
MS.gene06058.t1 MTR_1g093020 28.345 441 268 12 25 445 24 436 2.44e-38 144
MS.gene06058.t1 MTR_1g093020 29.353 402 250 10 25 413 24 404 1.49e-37 142
MS.gene06058.t1 MTR_3g085820 28.531 354 231 9 21 367 29 367 4.32e-37 140
MS.gene06058.t1 MTR_5g054730 28.222 450 277 13 15 445 16 438 1.43e-36 139
MS.gene06058.t1 MTR_3g115090 27.387 398 263 11 31 413 90 476 3.83e-36 139
MS.gene06058.t1 MTR_1g093030 27.945 433 272 12 29 445 28 436 6.95e-35 135
MS.gene06058.t1 MTR_2g096830 27.378 347 231 8 21 360 10 342 5.75e-34 130
MS.gene06058.t1 MTR_1g106000 25.427 468 297 13 24 448 14 472 2.89e-33 131
MS.gene06058.t1 MTR_1g105980 25.926 459 297 13 24 448 21 470 3.40e-33 130
MS.gene06058.t1 MTR_0531s0020 29.630 297 192 6 27 317 51 336 4.60e-33 128
MS.gene06058.t1 MTR_3g096800 27.051 451 283 17 31 448 39 476 4.84e-32 127
MS.gene06058.t1 MTR_3g088455 28.635 447 280 15 31 447 40 477 1.14e-30 124
MS.gene06058.t1 MTR_1g105995 25.708 459 296 15 23 446 19 467 3.62e-30 122
MS.gene06058.t1 MTR_3g096830 25.995 427 281 11 16 416 17 434 4.73e-30 122
MS.gene06058.t1 MTR_3g088475 28.348 448 281 15 31 447 40 478 6.35e-30 121
MS.gene06058.t1 MTR_1g029750 26.291 426 277 12 24 419 34 452 9.38e-29 118
MS.gene06058.t1 MTR_1g105990 25.517 435 275 14 24 420 20 443 1.87e-28 117
MS.gene06058.t1 MTR_5g023260 24.942 429 282 13 8 413 88 499 2.81e-28 117
MS.gene06058.t1 MTR_2g012470 26.667 435 281 14 32 446 102 518 1.62e-27 115
MS.gene06058.t1 MTR_3g110660 24.790 476 302 17 20 448 15 481 2.41e-27 114
MS.gene06058.t1 MTR_5g023240 24.893 466 314 14 2 448 64 512 3.52e-26 111
MS.gene06058.t1 MTR_1g007240 24.609 447 296 13 31 446 18 454 4.05e-26 110
MS.gene06058.t1 MTR_4g075750 24.711 433 294 11 33 443 78 500 4.22e-26 110
MS.gene06058.t1 MTR_4g073520 23.992 471 295 17 24 448 58 511 6.19e-26 110
MS.gene06058.t1 MTR_4g073520 23.992 471 295 17 24 448 58 511 7.48e-26 110
MS.gene06058.t1 MTR_3g096840 24.499 449 291 15 31 445 45 479 1.44e-25 109
MS.gene06058.t1 MTR_8g094290 24.609 447 299 13 31 448 37 474 1.56e-25 108
MS.gene06058.t1 MTR_1g007270 24.505 404 273 9 31 410 39 434 4.49e-24 104
MS.gene06058.t1 MTR_1g007380 24.945 457 298 17 27 448 25 471 6.36e-24 104
MS.gene06058.t1 MTR_3g069960 28.936 235 148 6 87 316 85 305 3.50e-23 100
MS.gene06058.t1 MTR_3g110430 25.971 412 264 9 31 411 67 468 9.47e-23 101
MS.gene06058.t1 MTR_5g096840 26.415 424 267 14 22 412 26 437 2.86e-22 99.4
MS.gene06058.t1 MTR_3g461440 25.641 390 255 13 36 397 34 416 1.78e-21 97.1
MS.gene06058.t1 MTR_1g007180 23.094 446 302 12 31 445 18 453 6.33e-21 95.1
MS.gene06058.t1 MTR_3g461430 25.635 394 260 12 36 402 41 428 1.41e-20 94.7
MS.gene06058.t1 MTR_3g061390 26.835 436 265 17 36 432 34 454 2.02e-20 94.0
MS.gene06058.t1 MTR_3g110420 23.094 459 311 11 16 445 22 467 5.19e-20 92.8
MS.gene06058.t1 MTR_3g461470 24.942 429 282 15 36 432 34 454 1.63e-19 91.3
MS.gene06058.t1 MTR_2g031750 23.111 450 297 14 31 445 18 453 9.30e-19 88.6
MS.gene06058.t1 MTR_5g096850 23.250 400 263 10 31 397 31 419 1.15e-17 85.5
MS.gene06058.t1 MTR_3g110660 24.545 330 209 12 20 317 15 336 2.18e-14 75.1
MS.gene06058.t1 MTR_1g007180 24.510 306 199 9 163 445 73 369 3.27e-14 74.3
MS.gene06058.t1 MTR_4g415390 24.887 442 276 16 31 445 49 461 6.05e-14 73.9
MS.gene06058.t1 MTR_3g088465 26.006 323 215 11 31 337 40 354 8.10e-14 73.2
MS.gene06058.t1 MTR_5g082220 24.945 453 268 22 31 445 42 460 3.60e-13 71.6
MS.gene06058.t1 MTR_5g082220 24.945 453 268 22 31 445 32 450 3.80e-13 71.6
MS.gene06058.t1 MTR_3g072870 24.558 452 279 16 27 445 32 454 1.32e-12 69.7
MS.gene06058.t1 MTR_4g415390 24.611 386 253 12 31 406 49 406 9.49e-12 67.0
MS.gene06058.t1 MTR_2g031750 24.194 310 195 11 163 445 90 386 3.06e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06058.t1 AT5G41800 80.420 429 84 0 23 451 24 452 0.0 718
MS.gene06058.t1 AT1G08230 45.047 424 232 1 23 445 25 448 1.17e-134 395
MS.gene06058.t1 AT1G08230 43.391 348 196 1 99 445 1 348 2.09e-101 306
MS.gene06058.t1 AT1G08230 43.902 328 183 1 119 445 2 329 6.61e-98 297
MS.gene06058.t1 AT1G08230 43.902 328 183 1 119 445 2 329 6.61e-98 297
MS.gene06058.t1 AT1G08230 43.902 328 183 1 119 445 2 329 6.61e-98 297
MS.gene06058.t1 AT5G40780 27.233 459 302 11 2 446 6 446 4.95e-51 179
MS.gene06058.t1 AT1G48640 27.391 460 300 12 2 446 13 453 1.47e-44 162
MS.gene06058.t1 AT2G39890 28.966 435 267 12 26 445 32 439 3.01e-44 160
MS.gene06058.t1 AT2G39890 28.966 435 267 12 26 445 32 439 3.01e-44 160
MS.gene06058.t1 AT1G24400 27.816 435 288 10 21 446 24 441 8.49e-44 159
MS.gene06058.t1 AT5G40780 27.885 416 272 10 41 446 1 398 8.91e-44 158
MS.gene06058.t1 AT5G40780 27.885 416 272 10 41 446 1 398 8.91e-44 158
MS.gene06058.t1 AT2G39890 29.250 400 250 10 56 445 4 380 2.03e-42 154
MS.gene06058.t1 AT1G67640 28.406 433 288 9 21 446 24 441 5.12e-42 154
MS.gene06058.t1 AT3G55740 28.736 435 268 12 26 445 29 436 1.08e-41 154
MS.gene06058.t1 AT2G36590 29.070 430 273 11 26 445 26 433 2.29e-41 152
MS.gene06058.t1 AT3G55740 29.000 400 251 10 56 445 4 380 6.62e-40 147
MS.gene06058.t1 AT3G55740 29.000 400 251 10 56 445 4 380 6.62e-40 147
MS.gene06058.t1 AT1G61270 27.363 402 268 10 21 413 29 415 4.45e-38 144
MS.gene06058.t1 AT1G25530 29.062 437 284 12 18 445 20 439 2.34e-36 139
MS.gene06058.t1 AT1G71680 27.021 433 295 9 21 446 30 448 3.53e-35 136
MS.gene06058.t1 AT3G01760 25.622 402 276 8 21 413 27 414 8.17e-33 129
MS.gene06058.t1 AT1G71680 27.378 347 231 9 21 360 30 362 4.73e-29 117
MS.gene06058.t1 AT1G44100 25.435 460 288 16 31 448 33 479 1.22e-27 115
MS.gene06058.t1 AT1G10010 25.829 422 276 10 27 418 30 444 1.59e-27 115
MS.gene06058.t1 AT1G10010 25.829 422 276 10 27 418 29 443 1.77e-27 114
MS.gene06058.t1 AT2G36590 29.195 298 186 8 26 316 26 305 6.14e-27 110
MS.gene06058.t1 AT5G49630 25.000 456 298 13 27 447 34 480 1.47e-25 109
MS.gene06058.t1 AT5G09220 24.615 455 291 14 31 447 51 491 2.07e-25 108
MS.gene06058.t1 AT1G77380 24.703 421 269 12 31 416 35 442 3.10e-25 108
MS.gene06058.t1 AT1G58360 24.561 456 298 13 27 447 38 482 4.19e-24 105
MS.gene06058.t1 AT1G47670 24.711 433 277 11 32 435 100 512 4.57e-24 105
MS.gene06058.t1 AT5G63850 23.160 462 303 14 24 447 17 464 9.55e-24 103
MS.gene06058.t1 AT5G23810 25.416 421 264 14 12 394 12 420 2.24e-18 87.8
MS.gene06058.t1 AT4G35180 23.235 439 289 13 21 439 53 463 2.20e-17 84.7
MS.gene06058.t1 AT4G35180 24.904 261 178 7 33 283 65 317 1.22e-12 69.3

Find 130 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACTCTGCTGCAGTTTC+TGG 0.055926 4.1:-4746686 MS.gene06058:CDS
CAAAATCATCATTGACTTAT+TGG 0.111694 4.1:-4746025 MS.gene06058:CDS
GTTTCTGGGTATTGGGTATT+TGG 0.176881 4.1:-4746671 MS.gene06058:CDS
CACTATCAAACAGCTTAAAT+TGG 0.193422 4.1:+4745916 None:intergenic
CAATAAGTCAATGATGATTT+TGG 0.212437 4.1:+4746026 None:intergenic
TTGGATAAAGATTACAAAAT+AGG 0.232346 4.1:+4748468 None:intergenic
TTTATCATCTTTCCAGTTGC+AGG 0.255166 4.1:+4746746 None:intergenic
CTGCTGCAGTTTCTGGGTAT+TGG 0.260317 4.1:-4746679 MS.gene06058:CDS
TGTAGCCCAAGCTGAGAAAA+AGG 0.265214 4.1:+4747183 None:intergenic
TGCTGCAGTTTCTGGGTATT+GGG 0.278167 4.1:-4746678 MS.gene06058:CDS
GCCAGATGATGGACCTTCTT+TGG 0.282256 4.1:-4746612 MS.gene06058:CDS
TTGGCTCCAACTTGGGTCCT+TGG 0.286614 4.1:-4746593 MS.gene06058:CDS
GCGAGGAAGAAGATTTCTTT+TGG 0.301221 4.1:+4746202 None:intergenic
TGCTGTTGGTTTCATTCCTC+TGG 0.308064 4.1:-4746093 MS.gene06058:CDS
GCAACTGGAAAGATGATAAA+AGG 0.310014 4.1:-4746743 MS.gene06058:CDS
CCGTACGCATTCAGAGGATT+AGG 0.313937 4.1:-4749259 MS.gene06058:CDS
TCAAGTTGCTTATGAGATAA+TGG 0.316818 4.1:-4746261 MS.gene06058:CDS
ACCTTCTTTGGCTCCAACTT+GGG 0.338327 4.1:-4746600 MS.gene06058:CDS
ATCAAGAACTAATTTCCTTA+TGG 0.340524 4.1:+4745944 None:intergenic
GGCTACACCTTCCTTGTAGT+TGG 0.342809 4.1:-4747167 MS.gene06058:CDS
TGCTCCATGTGGTGCAATAT+TGG 0.345478 4.1:+4747304 None:intergenic
CGAGTTCTCGGAAACGGATA+TGG 0.345658 4.1:+4749131 None:intergenic
GAACTCGCCGCCGATGTCTT+AGG 0.352198 4.1:-4749115 MS.gene06058:intron
TCTACTCTGCTGCAGTTTCT+GGG 0.376927 4.1:-4746685 MS.gene06058:CDS
CATTTAACAACGGCGATTGT+AGG 0.384219 4.1:-4749301 MS.gene06058:CDS
GACCTTCTTTGGCTCCAACT+TGG 0.385739 4.1:-4746601 MS.gene06058:CDS
CGCCGGTATTGATTGCCGTT+TGG 0.387244 4.1:+4748449 None:intergenic
AATTGCTGCAATTATGGAAA+TGG 0.392812 4.1:+4746956 None:intergenic
CATGAGGCAGTAAGAGTAGA+AGG 0.394794 4.1:+4749192 None:intergenic
GTAGGGGCAATCCTGCTTGC+TGG 0.394857 4.1:-4748421 MS.gene06058:CDS
CTTCTTCAACTCTTCGCAAT+CGG 0.397741 4.1:-4746551 MS.gene06058:CDS
TGGTGATTCTGATTCTCCTT+AGG 0.405695 4.1:+4749492 None:intergenic
TGTAAAGTGTTCGAAGAATC+AGG 0.407219 4.1:+4746180 None:intergenic
TATGTGTTATGCCGTAATTT+TGG 0.409726 4.1:-4746714 MS.gene06058:CDS
TTGGATCAACATTTCAATCA+TGG 0.409998 4.1:-4746006 MS.gene06058:CDS
ACTCTAGCACCTCCTGCAAC+TGG 0.420616 4.1:-4746758 MS.gene06058:CDS
CGGCGTCATCTTCATCTTGA+GGG 0.427559 4.1:+4749469 None:intergenic
AGACATCGGCGGCGAGTTCT+CGG 0.429362 4.1:+4749119 None:intergenic
ATGTTATGCCTAACAGTTAT+GGG 0.431198 4.1:-4749223 MS.gene06058:CDS
CGTACGCATTCAGAGGATTA+GGG 0.431714 4.1:-4749258 MS.gene06058:CDS
CATCAAACTCTTCAAAATGT+TGG 0.435510 4.1:+4746634 None:intergenic
TCAATCATGGTGGCATTCAC+TGG 0.438826 4.1:-4745993 MS.gene06058:CDS
GTAGAATGTCACCAAAATTA+CGG 0.442251 4.1:+4746703 None:intergenic
AAAATGGTTACTTGTATCTC+AGG 0.442563 4.1:+4746915 None:intergenic
TGAGATAATGGAGAAGAAAT+CGG 0.444629 4.1:-4746249 MS.gene06058:CDS
ATCCAAACGGCAATCAATAC+CGG 0.447790 4.1:-4748451 MS.gene06058:CDS
CTCCTTAGGTGGGTTTGCCA+TGG 0.450574 4.1:+4749506 None:intergenic
ACTCTTCAAAATGTTGGAGT+TGG 0.450754 4.1:+4746640 None:intergenic
AAGACCCTTGAGGATTCCTT+AGG 0.451852 4.1:+4746993 None:intergenic
AATGTTATGCCTAACAGTTA+TGG 0.453009 4.1:-4749224 MS.gene06058:CDS
GCCAAAGAAGGTCCATCATC+TGG 0.458042 4.1:+4746611 None:intergenic
TCAATACCGGCGTTGGCGTA+GGG 0.464596 4.1:-4748438 MS.gene06058:CDS
TCAACTCTTCGCAATCGGTC+TGG 0.469711 4.1:-4746546 MS.gene06058:intron
ATTCACTGGTGCAGGAATCA+TGG 0.473389 4.1:-4745979 MS.gene06058:CDS
CTGGAGGACGAAAGCAGCTC+CGG 0.474259 4.1:+4749449 None:intergenic
ATTTAACAACGGCGATTGTA+GGG 0.474479 4.1:-4749300 MS.gene06058:CDS
GGACTATTCATTGGAACCTA+AGG 0.477744 4.1:-4747009 MS.gene06058:CDS
TGATTCTGATTCTCCTTAGG+TGG 0.479279 4.1:+4749495 None:intergenic
TTACGGCATAACACATAGTA+AGG 0.482389 4.1:+4746720 None:intergenic
AAGAGTTTGATGCCAGATGA+TGG 0.482621 4.1:-4746623 MS.gene06058:CDS
GCTTGCTGGTCAATGCCTCC+AGG 0.485762 4.1:-4748407 MS.gene06058:intron
GATTCTGATTCTCCTTAGGT+GGG 0.485762 4.1:+4749496 None:intergenic
CTTGATCATTGCGAAAATGC+CGG 0.487042 4.1:-4749160 MS.gene06058:CDS
TGGGTGCATTCTCGTCCATA+AGG 0.489029 4.1:-4745959 MS.gene06058:CDS
AATGGCGTGGTTGGTGCTGT+TGG 0.491300 4.1:-4746107 MS.gene06058:CDS
TTACAAGACCCATAACTGTT+AGG 0.492363 4.1:+4749215 None:intergenic
AGTTGATGTGTCGTAGGGAA+TGG 0.492712 4.1:+4747213 None:intergenic
CCCTCCAAGGGACTATTCAT+TGG 0.493243 4.1:-4747018 MS.gene06058:CDS
ATGGTGGCATTCACTGGTGC+AGG 0.497413 4.1:-4745987 MS.gene06058:CDS
CCGGCGTCATCTTCATCTTG+AGG 0.503188 4.1:+4749468 None:intergenic
ATCAATACCGGCGTTGGCGT+AGG 0.512369 4.1:-4748439 MS.gene06058:CDS
CATTGATGACAGGGTCGGGT+TGG 0.517341 4.1:-4748495 MS.gene06058:intron
GGTTCCAATGAATAGTCCCT+TGG 0.519142 4.1:+4747014 None:intergenic
GAAACGGATATGGCGGCGAC+CGG 0.520639 4.1:+4749141 None:intergenic
TTCACTGGTGCAGGAATCAT+GGG 0.520800 4.1:-4745978 MS.gene06058:CDS
CATTCAGAGGATTAGGGTGG+GGG 0.524963 4.1:-4749252 MS.gene06058:CDS
TGTTGTATAAAATCATAGGC+AGG 0.525971 4.1:+4746063 None:intergenic
GTGTCGTAGGGAATGGAAAG+TGG 0.532912 4.1:+4747220 None:intergenic
TGTCGTAGGGAATGGAAAGT+GGG 0.547788 4.1:+4747221 None:intergenic
TACGGCAGCGTTAGTATCGT+TGG 0.548155 4.1:+4749277 None:intergenic
ACCCAAGTTGGAGCCAAAGA+AGG 0.550451 4.1:+4746599 None:intergenic
TGCCATGGCAAACCCACCTA+AGG 0.551622 4.1:-4749508 MS.gene06058:CDS
CGGCGGCGAGTTCTCGGAAA+CGG 0.551808 4.1:+4749125 None:intergenic
TCCAATGAATAGTCCCTTGG+AGG 0.554518 4.1:+4747017 None:intergenic
GCGAGTCCAAGGACCCAAGT+TGG 0.554753 4.1:+4746587 None:intergenic
GGAGACATCAATGGCGTGGT+TGG 0.554958 4.1:-4746116 MS.gene06058:CDS
GCATTCAGAGGATTAGGGTG+GGG 0.556854 4.1:-4749253 MS.gene06058:CDS
GTTGGTGCTTGTATTCATGC+AGG 0.557410 4.1:-4747149 MS.gene06058:intron
ACGGCAATCAATACCGGCGT+TGG 0.565368 4.1:-4748445 MS.gene06058:CDS
CGCATTCAGAGGATTAGGGT+GGG 0.565491 4.1:-4749254 MS.gene06058:CDS
ATCATCTTTCCAGTTGCAGG+AGG 0.565721 4.1:+4746749 None:intergenic
GGAGGCATTGACCAGCAAGC+AGG 0.567407 4.1:+4748410 None:intergenic
CAAAGACGATAGCGAGTCCA+AGG 0.572437 4.1:+4746576 None:intergenic
GAATACAAGCACCAACTACA+AGG 0.578514 4.1:+4747156 None:intergenic
GCTGTTTGATAGTGAAGTTG+TGG 0.589328 4.1:-4745907 MS.gene06058:CDS
CCTAATCCTCTGAATGCGTA+CGG 0.589330 4.1:+4749259 None:intergenic
CAATACCGGCGTTGGCGTAG+GGG 0.590022 4.1:-4748437 MS.gene06058:CDS
GATTACATCACCTAAGACAT+CGG 0.591318 4.1:+4749105 None:intergenic
ACGCTGCCGTACGCATTCAG+AGG 0.593272 4.1:-4749265 MS.gene06058:CDS
AATTATGGAAATGGCAGTGA+AGG 0.596045 4.1:+4746965 None:intergenic
AATTGATTGATATAGGTGAA+TGG 0.604073 4.1:-4749339 MS.gene06058:intron
TACTCCAATATTGCACCACA+TGG 0.605379 4.1:-4747308 MS.gene06058:CDS
AACACAAGTTGATGTGTCGT+AGG 0.607210 4.1:+4747207 None:intergenic
CCAATGAATAGTCCCTTGGA+GGG 0.611399 4.1:+4747018 None:intergenic
GATTGCCCCTACGCCAACGC+CGG 0.613136 4.1:+4748432 None:intergenic
AAATCGGCAGATGTGAAGCA+AGG 0.618331 4.1:-4746233 MS.gene06058:CDS
ACACAAGTTGATGTGTCGTA+GGG 0.621227 4.1:+4747208 None:intergenic
ACTGTACGACTTCATAGCAA+TGG 0.624920 4.1:-4747276 MS.gene06058:CDS
ACAAGCACCAACTACAAGGA+AGG 0.626871 4.1:+4747160 None:intergenic
TGGAACCTAAGGAATCCTCA+AGG 0.630547 4.1:-4746998 MS.gene06058:CDS
TATAGGTGAATGGTGGCACG+CGG 0.632366 4.1:-4749329 MS.gene06058:intron
ACGCATTCAGAGGATTAGGG+TGG 0.633907 4.1:-4749255 MS.gene06058:CDS
GCGTGGCTAAACTCATGCCA+TGG 0.638366 4.1:-4749523 None:intergenic
CGTCATCTTCATCTTGAGGG+TGG 0.638459 4.1:+4749472 None:intergenic
TAGGTGAATGGTGGCACGCG+GGG 0.647836 4.1:-4749327 MS.gene06058:CDS
ATAGGTGAATGGTGGCACGC+GGG 0.648320 4.1:-4749328 MS.gene06058:intron
GGAACCTAAGGAATCCTCAA+GGG 0.649619 4.1:-4746997 MS.gene06058:CDS
AATCGGCAGATGTGAAGCAA+GGG 0.649745 4.1:-4746232 MS.gene06058:CDS
GATCAACATTTCAATCATGG+TGG 0.650071 4.1:-4746003 MS.gene06058:CDS
TACAGTTTCAATGCTCCATG+TGG 0.654464 4.1:+4747293 None:intergenic
CAATGAATAGTCCCTTGGAG+GGG 0.654932 4.1:+4747019 None:intergenic
GATCAAGAACTTTAGACATG+AGG 0.658137 4.1:+4749176 None:intergenic
GTTCTCGGAAACGGATATGG+CGG 0.683221 4.1:+4749134 None:intergenic
CCTCAAGATGAAGATGACGC+CGG 0.694783 4.1:-4749468 MS.gene06058:CDS
GAAGGCACTGAAGACCCTTG+AGG 0.696373 4.1:+4746983 None:intergenic
CGCGGGGTATCATTTAACAA+CGG 0.699944 4.1:-4749311 MS.gene06058:CDS
ATAGGCAGGACAAAATCCAG+AGG 0.715818 4.1:+4746077 None:intergenic
TGATTGATATAGGTGAATGG+TGG 0.718250 4.1:-4749336 MS.gene06058:intron
TACATCACCTAAGACATCGG+CGG 0.726868 4.1:+4749108 None:intergenic
AGTGTTCGAAGAATCAGGCG+AGG 0.728618 4.1:+4746185 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAGAACAAAAAAAAAT+CGG + chr4.1:4747932-4747951 None:intergenic 10.0%
!! ACTATTTATCTTATAAAATA+TGG + chr4.1:4746671-4746690 None:intergenic 10.0%
!! ATAAAAAATTTAATATTGCA+CGG - chr4.1:4747088-4747107 MS.gene06058:intron 10.0%
!! GTAAAATTACTAAAAATAAT+TGG + chr4.1:4747038-4747057 None:intergenic 10.0%
!! TAATTATTAATGTTTGAAAA+TGG + chr4.1:4746408-4746427 None:intergenic 10.0%
!! AAAATGTATTCTATAAAAAG+AGG - chr4.1:4747807-4747826 MS.gene06058:intron 15.0%
!! AAATAAATAAGGGTAAAATA+GGG - chr4.1:4747115-4747134 MS.gene06058:intron 15.0%
!! AAATAATTTCCACAATTATT+TGG - chr4.1:4746591-4746610 MS.gene06058:CDS 15.0%
!! AATTATTTCAATTTAGAAAG+AGG + chr4.1:4748524-4748543 None:intergenic 15.0%
!! GACAAATATGTAAAATAAAT+AGG - chr4.1:4747742-4747761 MS.gene06058:intron 15.0%
!! TAAATAAATAAGGGTAAAAT+AGG - chr4.1:4747114-4747133 MS.gene06058:intron 15.0%
!! TAATATTTCAATTTGTGATT+TGG - chr4.1:4748061-4748080 MS.gene06058:intron 15.0%
!! TCATTATGAATTAATTGTAT+AGG - chr4.1:4748575-4748594 MS.gene06058:intron 15.0%
!! TTGTTCTTAATTAGTAATAT+AGG - chr4.1:4747940-4747959 MS.gene06058:intron 15.0%
!!! ATCAATAATTGTTTTTTTAG+AGG - chr4.1:4746489-4746508 MS.gene06058:intron 15.0%
!!! ATTTAAGATTTTCTATATTC+AGG - chr4.1:4746377-4746396 MS.gene06058:intron 15.0%
!!! ATTTAGAATGTTAAAATACT+TGG - chr4.1:4748016-4748035 MS.gene06058:intron 15.0%
!!! TGAATTTTCTTAAATTTTGT+TGG - chr4.1:4746890-4746909 MS.gene06058:intron 15.0%
!!! TTTAGTCTTATCATATTTAT+AGG - chr4.1:4749120-4749139 MS.gene06058:CDS 15.0%
!! AATAAATAAGGGTAAAATAG+GGG - chr4.1:4747116-4747135 MS.gene06058:intron 20.0%
!! ACAATTAATTCATAATGACA+TGG + chr4.1:4748573-4748592 None:intergenic 20.0%
!! AGATATGAATTGATTGATAT+AGG - chr4.1:4746065-4746084 MS.gene06058:CDS 20.0%
!! ATTAGATTACCAAATAATTG+TGG + chr4.1:4746603-4746622 None:intergenic 20.0%
!! ATTCAATACTAGTATGAAAT+AGG - chr4.1:4747462-4747481 MS.gene06058:intron 20.0%
!! TATGAATTATGATAACTCTT+AGG - chr4.1:4747547-4747566 MS.gene06058:intron 20.0%
!! TCAAGATAATTAAGAAAAAC+TGG - chr4.1:4747661-4747680 MS.gene06058:intron 20.0%
!! TGTAACATAAATGTAATCAA+GGG + chr4.1:4748323-4748342 None:intergenic 20.0%
!! TTGGATAAAGATTACAAAAT+AGG + chr4.1:4746946-4746965 None:intergenic 20.0%
!! TTGTAACATAAATGTAATCA+AGG + chr4.1:4748324-4748343 None:intergenic 20.0%
!!! AAACAATTATTGATTGATAG+AGG + chr4.1:4746483-4746502 None:intergenic 20.0%
!!! GACTTTAATTTCAATTTGTA+CGG + chr4.1:4746452-4746471 None:intergenic 20.0%
!!! GTTTTGTTGTATAAAATCAT+AGG + chr4.1:4749355-4749374 None:intergenic 20.0%
!!! TATTTTAGAACCATTATTTG+TGG + chr4.1:4747603-4747622 None:intergenic 20.0%
!!! TCTAGTATTTTTCTTGTATA+TGG + chr4.1:4747208-4747227 None:intergenic 20.0%
!!! TTCTTTTCTAATTCTTTTTC+TGG - chr4.1:4746353-4746372 MS.gene06058:intron 20.0%
!!! TTTTGTAATCTTTATCCAAA+CGG - chr4.1:4746947-4746966 MS.gene06058:CDS 20.0%
!!! TTTTTTTCTGCTTTTTTTGA+GGG - chr4.1:4747291-4747310 MS.gene06058:CDS 20.0%
!!! TTTTTTTTCTGCTTTTTTTG+AGG - chr4.1:4747290-4747309 MS.gene06058:CDS 20.0%
! AATCTAGCAATATATTTGTC+TGG + chr4.1:4748548-4748567 None:intergenic 25.0%
! AATTGATTGATATAGGTGAA+TGG - chr4.1:4746072-4746091 MS.gene06058:CDS 25.0%
! AATTTAGAAAGAGGACAAAA+TGG + chr4.1:4748515-4748534 None:intergenic 25.0%
! ACAAATATAAGGGATTGAAT+TGG - chr4.1:4748901-4748920 MS.gene06058:intron 25.0%
! AGAGTTATCATAATTCATAC+AGG + chr4.1:4747546-4747565 None:intergenic 25.0%
! ATCAAGAACTAATTTCCTTA+TGG + chr4.1:4749470-4749489 None:intergenic 25.0%
! CCATATCTTGTAATAAAACT+TGG + chr4.1:4746764-4746783 None:intergenic 25.0%
! GCACGGATTTAAATAAATAA+GGG - chr4.1:4747105-4747124 MS.gene06058:intron 25.0%
! GTAACATAAATGTAATCAAG+GGG + chr4.1:4748322-4748341 None:intergenic 25.0%
! TAACATAAATGTAATCAAGG+GGG + chr4.1:4748321-4748340 None:intergenic 25.0%
! TACTGTAATTGTTGATATTG+CGG - chr4.1:4748963-4748982 MS.gene06058:intron 25.0%
! TATATTCTCTCCACAAATAA+TGG - chr4.1:4747590-4747609 MS.gene06058:intron 25.0%
! TGAGTAATAAGTTAGTAACA+AGG - chr4.1:4746828-4746847 MS.gene06058:intron 25.0%
! TGCACGGATTTAAATAAATA+AGG - chr4.1:4747104-4747123 MS.gene06058:intron 25.0%
! TTAATTATCTTGATCTAGCA+TGG + chr4.1:4747654-4747673 None:intergenic 25.0%
!! CAATAAGTCAATGATGATTT+TGG + chr4.1:4749388-4749407 None:intergenic 25.0%
!! TATATGGAACTTTTCACATT+TGG - chr4.1:4747505-4747524 MS.gene06058:intron 25.0%
!! TTTCATACTAGTATTGAATG+TGG + chr4.1:4747461-4747480 None:intergenic 25.0%
!! TTTGTACGGTTTTTCTAATT+AGG + chr4.1:4746438-4746457 None:intergenic 25.0%
!!! AAGTGGGGTATTATATTTTT+TGG + chr4.1:4747238-4747257 None:intergenic 25.0%
!!! AGGCAAGAATTTTTTTCTAT+AGG - chr4.1:4747827-4747846 MS.gene06058:intron 25.0%
!!! AGTTTTATTACAAGATATGG+TGG - chr4.1:4746764-4746783 MS.gene06058:CDS 25.0%
!!! ATATCATTGATTCTAAGCAT+TGG + chr4.1:4746741-4746760 None:intergenic 25.0%
!!! CAAAATCATCATTGACTTAT+TGG - chr4.1:4749386-4749405 MS.gene06058:intron 25.0%
!!! CCAAGTTTTATTACAAGATA+TGG - chr4.1:4746761-4746780 MS.gene06058:CDS 25.0%
AAAAAACTAGTTGCTCTCAT+TGG + chr4.1:4747276-4747295 None:intergenic 30.0%
AAAATGGTTACTTGTATCTC+AGG + chr4.1:4748499-4748518 None:intergenic 30.0%
AAATAATTGGAAAGCTAACC+TGG + chr4.1:4747025-4747044 None:intergenic 30.0%
AAATACTTGGCTCAAGAAAA+TGG - chr4.1:4748029-4748048 MS.gene06058:intron 30.0%
AACGCATATCAGATAATGAA+TGG + chr4.1:4746538-4746557 None:intergenic 30.0%
AATGTTATGCCTAACAGTTA+TGG - chr4.1:4746187-4746206 MS.gene06058:CDS 30.0%
AATTGCTGCAATTATGGAAA+TGG + chr4.1:4748458-4748477 None:intergenic 30.0%
AGCTCACAAACACAAATATA+AGG - chr4.1:4748890-4748909 MS.gene06058:intron 30.0%
ATAGAAAAATTGCATGCCTT+TGG + chr4.1:4745989-4746008 None:intergenic 30.0%
ATCGTACAAATCTAGAAATG+CGG + chr4.1:4746869-4746888 None:intergenic 30.0%
ATGTTATGCCTAACAGTTAT+GGG - chr4.1:4746188-4746207 MS.gene06058:CDS 30.0%
CACTATCAAACAGCTTAAAT+TGG + chr4.1:4749498-4749517 None:intergenic 30.0%
CATCAAACTCTTCAAAATGT+TGG + chr4.1:4748780-4748799 None:intergenic 30.0%
GCGATTAAACTTGATATTGT+AGG - chr4.1:4748355-4748374 MS.gene06058:intron 30.0%
GCTCACAAACACAAATATAA+GGG - chr4.1:4748891-4748910 MS.gene06058:intron 30.0%
GTAGAATGTCACCAAAATTA+CGG + chr4.1:4748711-4748730 None:intergenic 30.0%
TGTTGTATAAAATCATAGGC+AGG + chr4.1:4749351-4749370 None:intergenic 30.0%
TTGGATCAACATTTCAATCA+TGG - chr4.1:4749405-4749424 MS.gene06058:intron 30.0%
! ACACTTTACATGATTTTCTG+TGG - chr4.1:4749244-4749263 MS.gene06058:CDS 30.0%
! ATACTAGTATTGAATGTGGA+TGG + chr4.1:4747457-4747476 None:intergenic 30.0%
! CCATAATTGCAGCAATTTTT+GGG - chr4.1:4748461-4748480 MS.gene06058:CDS 30.0%
! TAAGTCAATGATGATTTTGG+AGG + chr4.1:4749385-4749404 None:intergenic 30.0%
! TACAAGTTTGTTTGTAATCG+CGG + chr4.1:4748939-4748958 None:intergenic 30.0%
! TATGTGTTATGCCGTAATTT+TGG - chr4.1:4748697-4748716 MS.gene06058:intron 30.0%
! TCAAGTTGCTTATGAGATAA+TGG - chr4.1:4749150-4749169 MS.gene06058:CDS 30.0%
! TCCATAATTGCAGCAATTTT+TGG - chr4.1:4748460-4748479 MS.gene06058:CDS 30.0%
! TGGTTTTCATTCAACATAGA+CGG - chr4.1:4747348-4747367 MS.gene06058:intron 30.0%
!! GATTGAATTGGTTTGAACTA+TGG - chr4.1:4748913-4748932 MS.gene06058:intron 30.0%
!! TGAGATAATGGAGAAGAAAT+CGG - chr4.1:4749162-4749181 MS.gene06058:CDS 30.0%
!!! TTTTATTTTGCTTCCGATAG+TGG - chr4.1:4747877-4747896 MS.gene06058:intron 30.0%
AATTATGGAAATGGCAGTGA+AGG + chr4.1:4748449-4748468 None:intergenic 35.0%
ACTCTTCAAAATGTTGGAGT+TGG + chr4.1:4748774-4748793 None:intergenic 35.0%
AGTGAGTGACAATCTGAAAA+CGG - chr4.1:4747395-4747414 MS.gene06058:intron 35.0%
ATTTAACAACGGCGATTGTA+GGG - chr4.1:4746111-4746130 MS.gene06058:CDS 35.0%
CATATACACCATAGTGATAG+GGG + chr4.1:4747491-4747510 None:intergenic 35.0%
CCATATACACCATAGTGATA+GGG + chr4.1:4747492-4747511 None:intergenic 35.0%
CCATTGATGTCTCCAAAAAA+CGG + chr4.1:4749289-4749308 None:intergenic 35.0%
CCCAAAAATTGCTGCAATTA+TGG + chr4.1:4748464-4748483 None:intergenic 35.0%
GAGAAGAAACACTCAACAAA+AGG + chr4.1:4746707-4746726 None:intergenic 35.0%
GATCAACATTTCAATCATGG+TGG - chr4.1:4749408-4749427 MS.gene06058:intron 35.0%
GATCAAGAACTTTAGACATG+AGG + chr4.1:4746238-4746257 None:intergenic 35.0%
GATTACATCACCTAAGACAT+CGG + chr4.1:4746309-4746328 None:intergenic 35.0%
GCAACTGGAAAGATGATAAA+AGG - chr4.1:4748668-4748687 MS.gene06058:intron 35.0%
TCCATATACACCATAGTGAT+AGG + chr4.1:4747493-4747512 None:intergenic 35.0%
TGATTGATATAGGTGAATGG+TGG - chr4.1:4746075-4746094 MS.gene06058:CDS 35.0%
TTACAAGACCCATAACTGTT+AGG + chr4.1:4746199-4746218 None:intergenic 35.0%
TTACGGCATAACACATAGTA+AGG + chr4.1:4748694-4748713 None:intergenic 35.0%
TTTATCATCTTTCCAGTTGC+AGG + chr4.1:4748668-4748687 None:intergenic 35.0%
! TGTAAAGTGTTCGAAGAATC+AGG + chr4.1:4749234-4749253 None:intergenic 35.0%
!! ATTGCAAAGGCTTTGGAAAT+TGG + chr4.1:4749043-4749062 None:intergenic 35.0%
!! ATTGCAGCAATTTTTGGGAA+TGG - chr4.1:4748466-4748485 MS.gene06058:CDS 35.0%
!! TGTTGGTTTCATTGATGACA+GGG - chr4.1:4746907-4746926 MS.gene06058:intron 35.0%
!! TTGTTGGTTTCATTGATGAC+AGG - chr4.1:4746906-4746925 MS.gene06058:intron 35.0%
AAAATTGCATGCCTTTGGAC+TGG + chr4.1:4745984-4746003 None:intergenic 40.0%
AAAGATCCGACATCCACTAT+CGG + chr4.1:4747893-4747912 None:intergenic 40.0%
AAGAGTTTGATGCCAGATGA+TGG - chr4.1:4748788-4748807 MS.gene06058:intron 40.0%
ACTGTACGACTTCATAGCAA+TGG - chr4.1:4748135-4748154 MS.gene06058:intron 40.0%
AGTGTTTCTTCTCTCTCGTA+AGG - chr4.1:4746714-4746733 MS.gene06058:CDS 40.0%
ATCAAAAAATGCCCCTCCAA+GGG - chr4.1:4748381-4748400 MS.gene06058:intron 40.0%
ATCCAAACGGCAATCAATAC+CGG - chr4.1:4746960-4746979 MS.gene06058:CDS 40.0%
CACATTTGGTCCACTTTGTT+TGG - chr4.1:4747519-4747538 MS.gene06058:intron 40.0%
CATTTAACAACGGCGATTGT+AGG - chr4.1:4746110-4746129 MS.gene06058:CDS 40.0%
CCCTATCACTATGGTGTATA+TGG - chr4.1:4747489-4747508 MS.gene06058:intron 40.0%
CTTCTTCAACTCTTCGCAAT+CGG - chr4.1:4748860-4748879 MS.gene06058:intron 40.0%
CTTGATCATTGCGAAAATGC+CGG - chr4.1:4746251-4746270 MS.gene06058:CDS 40.0%
GAATACAAGCACCAACTACA+AGG + chr4.1:4748258-4748277 None:intergenic 40.0%
GATTCTGATTCTCCTTAGGT+GGG + chr4.1:4745918-4745937 None:intergenic 40.0%
GGACTATTCATTGGAACCTA+AGG - chr4.1:4748402-4748421 MS.gene06058:intron 40.0%
GTAGTTGCTATAAGTCGTAG+AGG - chr4.1:4749014-4749033 MS.gene06058:intron 40.0%
GTCTTTGCAGGTTGCAATTT+AGG - chr4.1:4749069-4749088 MS.gene06058:intron 40.0%
GTGAGTGACAATCTGAAAAC+GGG - chr4.1:4747396-4747415 MS.gene06058:intron 40.0%
GTTGATATTGCGGACAAATG+CGG - chr4.1:4748973-4748992 MS.gene06058:intron 40.0%
TAATTGGAAAGCTAACCTGG+AGG + chr4.1:4747022-4747041 None:intergenic 40.0%
TACAGTTTCAATGCTCCATG+TGG + chr4.1:4748121-4748140 None:intergenic 40.0%
TACTCCAATATTGCACCACA+TGG - chr4.1:4748103-4748122 MS.gene06058:intron 40.0%
TCATACAGGTCCAAACAAAG+TGG + chr4.1:4747532-4747551 None:intergenic 40.0%
TGATTCTGATTCTCCTTAGG+TGG + chr4.1:4745919-4745938 None:intergenic 40.0%
TGCTTGTATTCATGCAGGTA+TGG - chr4.1:4748267-4748286 MS.gene06058:intron 40.0%
TGGTGATTCTGATTCTCCTT+AGG + chr4.1:4745922-4745941 None:intergenic 40.0%
! AGTGGATGTCGGATCTTTTA+TGG - chr4.1:4747895-4747914 MS.gene06058:intron 40.0%
! GCGAGGAAGAAGATTTCTTT+TGG + chr4.1:4749212-4749231 None:intergenic 40.0%
! GTCGGATCTTTTATGGAACA+GGG - chr4.1:4747902-4747921 MS.gene06058:intron 40.0%
! GTTGTTATTGTAGACACGCA+TGG - chr4.1:4747328-4747347 MS.gene06058:intron 40.0%
! TCGGATCTTTTATGGAACAG+GGG - chr4.1:4747903-4747922 MS.gene06058:intron 40.0%
! TGTCGGATCTTTTATGGAAC+AGG - chr4.1:4747901-4747920 MS.gene06058:intron 40.0%
! TGTTCCCTTTTTCTCAGCTT+GGG - chr4.1:4748223-4748242 MS.gene06058:intron 40.0%
!! AACACAAGTTGATGTGTCGT+AGG + chr4.1:4748207-4748226 None:intergenic 40.0%
!! ACACAAGTTGATGTGTCGTA+GGG + chr4.1:4748206-4748225 None:intergenic 40.0%
!! CTCATTGGTGTCACTATCAT+GGG + chr4.1:4747261-4747280 None:intergenic 40.0%
!! GCTGTTTGATAGTGAAGTTG+TGG - chr4.1:4749504-4749523 MS.gene06058:CDS 40.0%
!! GTTTCTGGGTATTGGGTATT+TGG - chr4.1:4748740-4748759 MS.gene06058:intron 40.0%
!! TCTCATTGGTGTCACTATCA+TGG + chr4.1:4747262-4747281 None:intergenic 40.0%
!!! CCGTTTTTTGGAGACATCAA+TGG - chr4.1:4749286-4749305 MS.gene06058:CDS 40.0%
AAATCGGCAGATGTGAAGCA+AGG - chr4.1:4749178-4749197 MS.gene06058:CDS 45.0%
AAGACCCTTGAGGATTCCTT+AGG + chr4.1:4748421-4748440 None:intergenic 45.0%
AATCGGCAGATGTGAAGCAA+GGG - chr4.1:4749179-4749198 MS.gene06058:CDS 45.0%
ACAAGCACCAACTACAAGGA+AGG + chr4.1:4748254-4748273 None:intergenic 45.0%
AGCATGGACAAGCTAACAAG+TGG + chr4.1:4747638-4747657 None:intergenic 45.0%
ATAGGCAGGACAAAATCCAG+AGG + chr4.1:4749337-4749356 None:intergenic 45.0%
ATAGGTGACCCCTATCACTA+TGG - chr4.1:4747480-4747499 MS.gene06058:intron 45.0%
ATCATCTTTCCAGTTGCAGG+AGG + chr4.1:4748665-4748684 None:intergenic 45.0%
ATTCACTGGTGCAGGAATCA+TGG - chr4.1:4749432-4749451 MS.gene06058:intron 45.0%
CAAAGACCGTCACATTGCAA+AGG + chr4.1:4749056-4749075 None:intergenic 45.0%
CAATGAATAGTCCCTTGGAG+GGG + chr4.1:4748395-4748414 None:intergenic 45.0%
CATCAAAAAATGCCCCTCCA+AGG - chr4.1:4748380-4748399 MS.gene06058:intron 45.0%
CATGAGGCAGTAAGAGTAGA+AGG + chr4.1:4746222-4746241 None:intergenic 45.0%
CCAAAGCCTTTGCAATGTGA+CGG - chr4.1:4749047-4749066 MS.gene06058:intron 45.0%
CCAATGAATAGTCCCTTGGA+GGG + chr4.1:4748396-4748415 None:intergenic 45.0%
CCTAATCCTCTGAATGCGTA+CGG + chr4.1:4746155-4746174 None:intergenic 45.0%
CGTACGCATTCAGAGGATTA+GGG - chr4.1:4746153-4746172 MS.gene06058:CDS 45.0%
GACAATCTGAAAACGGGTGA+TGG - chr4.1:4747402-4747421 MS.gene06058:intron 45.0%
GGAACCTAAGGAATCCTCAA+GGG - chr4.1:4748414-4748433 MS.gene06058:CDS 45.0%
GGTTCCAATGAATAGTCCCT+TGG + chr4.1:4748400-4748419 None:intergenic 45.0%
GTAGCCCAAGCTGAGAAAAA+GGG + chr4.1:4748230-4748249 None:intergenic 45.0%
TACATCACCTAAGACATCGG+CGG + chr4.1:4746306-4746325 None:intergenic 45.0%
TCAATCATGGTGGCATTCAC+TGG - chr4.1:4749418-4749437 MS.gene06058:intron 45.0%
TCCAATGAATAGTCCCTTGG+AGG + chr4.1:4748397-4748416 None:intergenic 45.0%
TCTACTCTGCTGCAGTTTCT+GGG - chr4.1:4748726-4748745 MS.gene06058:intron 45.0%
TGCTCCATGTGGTGCAATAT+TGG + chr4.1:4748110-4748129 None:intergenic 45.0%
TGGAACCTAAGGAATCCTCA+AGG - chr4.1:4748413-4748432 MS.gene06058:CDS 45.0%
TGTAGCCCAAGCTGAGAAAA+AGG + chr4.1:4748231-4748250 None:intergenic 45.0%
TGTCACTATCATGGGAAGTG+GGG + chr4.1:4747253-4747272 None:intergenic 45.0%
TGTCGTAGGGAATGGAAAGT+GGG + chr4.1:4748193-4748212 None:intergenic 45.0%
TTCACTGGTGCAGGAATCAT+GGG - chr4.1:4749433-4749452 MS.gene06058:intron 45.0%
TTCTACTCTGCTGCAGTTTC+TGG - chr4.1:4748725-4748744 MS.gene06058:intron 45.0%
! ACCTTCTTTGGCTCCAACTT+GGG - chr4.1:4748811-4748830 MS.gene06058:intron 45.0%
! AGTTGATGTGTCGTAGGGAA+TGG + chr4.1:4748201-4748220 None:intergenic 45.0%
! CGCGGGGTATCATTTAACAA+CGG - chr4.1:4746100-4746119 MS.gene06058:CDS 45.0%
! GGTTTCATTGATGACAGGGT+CGG - chr4.1:4746911-4746930 MS.gene06058:intron 45.0%
! GTGTCACTATCATGGGAAGT+GGG + chr4.1:4747254-4747273 None:intergenic 45.0%
! GTGTTCCCTTTTTCTCAGCT+TGG - chr4.1:4748222-4748241 MS.gene06058:intron 45.0%
! GTTGGTGCTTGTATTCATGC+AGG - chr4.1:4748262-4748281 MS.gene06058:intron 45.0%
! GTTTCATTGATGACAGGGTC+GGG - chr4.1:4746912-4746931 MS.gene06058:intron 45.0%
! TGCTGCAGTTTCTGGGTATT+GGG - chr4.1:4748733-4748752 MS.gene06058:intron 45.0%
!! TGCTGTTGGTTTCATTCCTC+TGG - chr4.1:4749318-4749337 MS.gene06058:CDS 45.0%
!! TTGCTTCCGATAGTGGATGT+CGG - chr4.1:4747884-4747903 MS.gene06058:intron 45.0%
!! TTTTGGAGACATCAATGGCG+TGG - chr4.1:4749291-4749310 MS.gene06058:CDS 45.0%
!!! GCAGCTATGTTGCCGTTTTT+TGG - chr4.1:4749274-4749293 MS.gene06058:CDS 45.0%
ACGCATTCAGAGGATTAGGG+TGG - chr4.1:4746156-4746175 MS.gene06058:CDS 50.0%
AGTGTTCGAAGAATCAGGCG+AGG + chr4.1:4749229-4749248 None:intergenic 50.0%
CAAAGACGATAGCGAGTCCA+AGG + chr4.1:4748838-4748857 None:intergenic 50.0%
CATTCAGAGGATTAGGGTGG+GGG - chr4.1:4746159-4746178 MS.gene06058:CDS 50.0%
CCCTCCAAGGGACTATTCAT+TGG - chr4.1:4748393-4748412 MS.gene06058:intron 50.0%
CCGTACGCATTCAGAGGATT+AGG - chr4.1:4746152-4746171 MS.gene06058:CDS 50.0%
CCGTCACATTGCAAAGGCTT+TGG + chr4.1:4749050-4749069 None:intergenic 50.0%
CCTCAAGATGAAGATGACGC+CGG - chr4.1:4745943-4745962 MS.gene06058:CDS 50.0%
CGAGTTCTCGGAAACGGATA+TGG + chr4.1:4746283-4746302 None:intergenic 50.0%
CGCATTCAGAGGATTAGGGT+GGG - chr4.1:4746157-4746176 MS.gene06058:CDS 50.0%
CGGCGTCATCTTCATCTTGA+GGG + chr4.1:4745945-4745964 None:intergenic 50.0%
CGTCATCTTCATCTTGAGGG+TGG + chr4.1:4745942-4745961 None:intergenic 50.0%
GAAATGCGGTTCTGCACGAT+TGG + chr4.1:4746855-4746874 None:intergenic 50.0%
GCATTCAGAGGATTAGGGTG+GGG - chr4.1:4746158-4746177 MS.gene06058:CDS 50.0%
GCCAAAGAAGGTCCATCATC+TGG + chr4.1:4748803-4748822 None:intergenic 50.0%
GGCTACACCTTCCTTGTAGT+TGG - chr4.1:4748244-4748263 MS.gene06058:intron 50.0%
GTGTCGTAGGGAATGGAAAG+TGG + chr4.1:4748194-4748213 None:intergenic 50.0%
GTTCTCGGAAACGGATATGG+CGG + chr4.1:4746280-4746299 None:intergenic 50.0%
TACGGCAGCGTTAGTATCGT+TGG + chr4.1:4746137-4746156 None:intergenic 50.0%
TATAGGTGAATGGTGGCACG+CGG - chr4.1:4746082-4746101 MS.gene06058:CDS 50.0%
TCAACTCTTCGCAATCGGTC+TGG - chr4.1:4748865-4748884 MS.gene06058:intron 50.0%
TGCAATGTGACGGTCTTTGC+AGG - chr4.1:4749057-4749076 MS.gene06058:intron 50.0%
TGGGTGCATTCTCGTCCATA+AGG - chr4.1:4749452-4749471 MS.gene06058:CDS 50.0%
! ACCCAAGTTGGAGCCAAAGA+AGG + chr4.1:4748815-4748834 None:intergenic 50.0%
! ATTGCATGCCTTTGGACTGG+AGG + chr4.1:4745981-4746000 None:intergenic 50.0%
! CTGCTGCAGTTTCTGGGTAT+TGG - chr4.1:4748732-4748751 MS.gene06058:intron 50.0%
! GACCTTCTTTGGCTCCAACT+TGG - chr4.1:4748810-4748829 MS.gene06058:intron 50.0%
!! GCCAGATGATGGACCTTCTT+TGG - chr4.1:4748799-4748818 MS.gene06058:intron 50.0%
!! GGTGTCACTATCATGGGAAG+TGG + chr4.1:4747255-4747274 None:intergenic 50.0%
ACGGCAATCAATACCGGCGT+TGG - chr4.1:4746966-4746985 MS.gene06058:CDS 55.0%
ACTCTAGCACCTCCTGCAAC+TGG - chr4.1:4748653-4748672 MS.gene06058:intron 55.0%
AGAGGATTAGGGTGGGGGAT+TGG - chr4.1:4746164-4746183 MS.gene06058:CDS 55.0%
ATAGGTGAATGGTGGCACGC+GGG - chr4.1:4746083-4746102 MS.gene06058:CDS 55.0%
ATGGTGGCATTCACTGGTGC+AGG - chr4.1:4749424-4749443 MS.gene06058:intron 55.0%
CCGGCGTCATCTTCATCTTG+AGG + chr4.1:4745946-4745965 None:intergenic 55.0%
GCTTTCGTCCTCCAGTCCAA+AGG - chr4.1:4745970-4745989 MS.gene06058:CDS 55.0%
GGAGACATCAATGGCGTGGT+TGG - chr4.1:4749295-4749314 MS.gene06058:CDS 55.0%
TGCCATGGCAAACCCACCTA+AGG - chr4.1:4745903-4745922 MS.gene06058:CDS 55.0%
TTGGCTCCAACTTGGGTCCT+TGG - chr4.1:4748818-4748837 MS.gene06058:intron 55.0%
! CATTGATGACAGGGTCGGGT+TGG - chr4.1:4746916-4746935 MS.gene06058:intron 55.0%
! CTCCTTAGGTGGGTTTGCCA+TGG + chr4.1:4745908-4745927 None:intergenic 55.0%
!! AATGGCGTGGTTGGTGCTGT+TGG - chr4.1:4749304-4749323 MS.gene06058:CDS 55.0%
!! ATCAATACCGGCGTTGGCGT+AGG - chr4.1:4746972-4746991 MS.gene06058:CDS 55.0%
!! CGCCGGTATTGATTGCCGTT+TGG + chr4.1:4746965-4746984 None:intergenic 55.0%
!! GAAGGCACTGAAGACCCTTG+AGG + chr4.1:4748431-4748450 None:intergenic 55.0%
!! TCAATACCGGCGTTGGCGTA+GGG - chr4.1:4746973-4746992 MS.gene06058:CDS 55.0%
ACGCTGCCGTACGCATTCAG+AGG - chr4.1:4746146-4746165 MS.gene06058:CDS 60.0%
AGACATCGGCGGCGAGTTCT+CGG + chr4.1:4746295-4746314 None:intergenic 60.0%
CTGGAGGACGAAAGCAGCTC+CGG + chr4.1:4745965-4745984 None:intergenic 60.0%
GAAACGGATATGGCGGCGAC+CGG + chr4.1:4746273-4746292 None:intergenic 60.0%
GAACTCGCCGCCGATGTCTT+AGG - chr4.1:4746296-4746315 MS.gene06058:intron 60.0%
GCGAGTCCAAGGACCCAAGT+TGG + chr4.1:4748827-4748846 None:intergenic 60.0%
GTAGGGGCAATCCTGCTTGC+TGG - chr4.1:4746990-4747009 MS.gene06058:CDS 60.0%
TAGGTGAATGGTGGCACGCG+GGG - chr4.1:4746084-4746103 MS.gene06058:CDS 60.0%
! GCTTGCTGGTCAATGCCTCC+AGG - chr4.1:4747004-4747023 MS.gene06058:CDS 60.0%
!! CAATACCGGCGTTGGCGTAG+GGG - chr4.1:4746974-4746993 MS.gene06058:CDS 60.0%
!! GGAGGCATTGACCAGCAAGC+AGG + chr4.1:4747004-4747023 None:intergenic 60.0%
CGGCGGCGAGTTCTCGGAAA+CGG + chr4.1:4746289-4746308 None:intergenic 65.0%
GATTGCCCCTACGCCAACGC+CGG + chr4.1:4746982-4747001 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 4745902 4749531 4745902 ID=MS.gene06058
chr4.1 mRNA 4745902 4749531 4745902 ID=MS.gene06058.t1;Parent=MS.gene06058
chr4.1 exon 4749450 4749531 4749450 ID=MS.gene06058.t1.exon1;Parent=MS.gene06058.t1
chr4.1 CDS 4749450 4749531 4749450 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
chr4.1 exon 4749116 4749346 4749116 ID=MS.gene06058.t1.exon2;Parent=MS.gene06058.t1
chr4.1 CDS 4749116 4749346 4749116 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
chr4.1 exon 4748408 4748505 4748408 ID=MS.gene06058.t1.exon3;Parent=MS.gene06058.t1
chr4.1 CDS 4748408 4748505 4748408 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
chr4.1 exon 4747150 4747336 4747150 ID=MS.gene06058.t1.exon4;Parent=MS.gene06058.t1
chr4.1 CDS 4747150 4747336 4747150 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
chr4.1 exon 4746926 4747056 4746926 ID=MS.gene06058.t1.exon5;Parent=MS.gene06058.t1
chr4.1 CDS 4746926 4747056 4746926 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
chr4.1 exon 4746547 4746783 4746547 ID=MS.gene06058.t1.exon6;Parent=MS.gene06058.t1
chr4.1 CDS 4746547 4746783 4746547 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
chr4.1 exon 4745902 4746291 4745902 ID=MS.gene06058.t1.exon7;Parent=MS.gene06058.t1
chr4.1 CDS 4745902 4746291 4745902 ID=cds.MS.gene06058.t1;Parent=MS.gene06058.t1
Gene Sequence

>MS.gene06058

ATGCCATGGCAAACCCACCTAAGGAGAATCAGAATCACCACCCTCAAGATGAAGATGACGCCGGAGCTGCTTTCGTCCTCCAGTCCAAAGGCATGCAATTTTTCTATATTCTTTAAATTCATCTTTAATGATTCATTAAATATCTATCTATCTATCTATCTAAAGATATGAATTGATTGATATAGGTGAATGGTGGCACGCGGGGTATCATTTAACAACGGCGATTGTAGGGCCAACGATACTAACGCTGCCGTACGCATTCAGAGGATTAGGGTGGGGGATTGGAATGTTATGCCTAACAGTTATGGGTCTTGTAACCTTCTACTCTTACTGCCTCATGTCTAAAGTTCTTGATCATTGCGAAAATGCCGGTCGCCGCCATATCCGTTTCCGAGAACTCGCCGCCGATGTCTTAGGTGATGTAATCTTTACTTTGCACGTTTGCATTGTGTTCTTTTCTAATTCTTTTTCTGGTATTTAAGATTTTCTATATTCAGGATATGCCATTTTCAAACATTAATAATTACTACTTACCTAATTAGAAAAACCGTACAAATTGAAATTAAAGTCAAACTGTTCCTCTATCAATCAATAATTGTTTTTTTAGAGGATCAATCAATAATTGTTATTTCACCATTCATTATCTGATATGCGTTAATAGTTGATTTATTCATCTACATGTTATATTAAAATAATTTCCACAATTATTTGGTAATCTAATCTCATTTAATTAATTAAAAAATGTATATGAAAATTAAAATATGCACCATATTTTATAAGATAAATAGTTTTTTTTTTCTTTCCTTTTGTTGAGTGTTTCTTCTCTCTCGTAAGGACCAATGCTTAGAATCAATGATATCCAAGTTTTATTACAAGATATGGTGGTGTGTACTGTGTACATAACACAACACAAGTGATATTTTTAATGAGTAATAAGTTAGTAACAAGGACCAATCGTGCAGAACCGCATTTCTAGATTTGTACGATATGAATTTTCTTAAATTTTGTTGGTTTCATTGATGACAGGGTCGGGTTGGATGTCCTATTTTGTAATCTTTATCCAAACGGCAATCAATACCGGCGTTGGCGTAGGGGCAATCCTGCTTGCTGGTCAATGCCTCCAGGTTAGCTTTCCAATTATTTTTAGTAATTTTACTTTACTAATATACTTTAATATTTGCTCAAAATAAAAAATTTAATATTGCACGGATTTAAATAAATAAGGGTAAAATAGGGGAAATTCTTTGTCTTTTTCTATCATTTGACATACATTTTATATAGCGTCATTCTCAAATCAAAGATACCATATACAAGAAAAATACTAGAATTTTTACCAAAAAATATAATACCCCACTTCCCATGATAGTGACACCAATGAGAGCAACTAGTTTTTTTTCTGCTTTTTTTGAGGGATTGAAAGCAACTAGTTGTTATTGTAGACACGCATGGTTTTCATTCAACATAGACGGACACATAATGATCATATTGATCATAGTGAGTGACAATCTGAAAACGGGTGATGGTTGTCGTTGCGAAGATGACAAGTTAAATTCCATCCACATTCAATACTAGTATGAAATAGGTGACCCCTATCACTATGGTGTATATGGAACTTTTCACATTTGGTCCACTTTGTTTGGACCTGTATGAATTATGATAACTCTTAGGCTCAGCTAAGTTCGTATATCTATATTCTCTCCACAAATAATGGTTCTAAAATATTGTACTATGTACCACTTGTTAGCTTGTCCATGCTAGATCAAGATAATTAAGAAAAACTGGTAATGTTATTTATTAAATTTAATAACAATTTTGTATTTAATATTTATTGTTGATTATAGACAAATATGTAAAATAAATAGGAGCATGTAAGTAAAAATAATAGTTAATGCAATTAAAATTATAAAAATGTATTCTATAAAAAGAGGCAAGAATTTTTTTCTATAGGTTCTAGATTTGAGAACACAGCAGTCATTTTTATTTTGCTTCCGATAGTGGATGTCGGATCTTTTATGGAACAGGGGAGTCCGATTTTTTTTTGTTCTTAATTAGTAATATAGGTATGTATAATGTATATACACATTTTGTAGCTAAAAAATCACTAATAAACAATGATTTAGAATGTTAAAATACTTGGCTCAAGAAAATGGCGTTCGTTTTAATATTTCAATTTGTGATTTGGTATTTTCTTTTAGATCGTGTACTCCAATATTGCACCACATGGAGCATTGAAACTGTACGACTTCATAGCAATGGTGACAGTAGTCATGATAATTTTGTCTCAGCTTCCCACTTTCCATTCCCTACGACACATCAACTTGTGTTCCCTTTTTCTCAGCTTGGGCTACACCTTCCTTGTAGTTGGTGCTTGTATTCATGCAGGTATGGACTACTAGTATATATTACAAAATTATTACCCCCTTGATTACATTTATGTTACAAATATTCATTATGCGATTAAACTTGATATTGTAGGTACATCAAAAAATGCCCCTCCAAGGGACTATTCATTGGAACCTAAGGAATCCTCAAGGGTCTTCAGTGCCTTCACTGCCATTTCCATAATTGCAGCAATTTTTGGGAATGGCATATTACCTGAGATACAAGTAACCATTTTGTCCTCTTTCTAAATTGAAATAATTACCAGACAAATATATTGCTAGATTAACCATGTCATTATGAATTAATTGTATTTACCAGACAAATATATTGCTAGATTAACCATGTCATTATGAATTAATTGTATAGGCAACTCTAGCACCTCCTGCAACTGGAAAGATGATAAAAGGCCTTACTATGTGTTATGCCGTAATTTTGGTGACATTCTACTCTGCTGCAGTTTCTGGGTATTGGGTATTTGGAAACAAGTCCAACTCCAACATTTTGAAGAGTTTGATGCCAGATGATGGACCTTCTTTGGCTCCAACTTGGGTCCTTGGACTCGCTATCGTCTTTGTTCTTCTTCAACTCTTCGCAATCGGTCTGGTAAGCTCACAAACACAAATATAAGGGATTGAATTGGTTTGAACTATGGCCGCGATTACAAACAAACTTGTAATTATACTGTAATTGTTGATATTGCGGACAAATGCGGATAAATGCAACTACAATTGTAGTTGCTATAAGTCGTAGAGGTTTCCAATTTCCAAAGCCTTTGCAATGTGACGGTCTTTGCAGGTTGCAATTTAGGACTATACTAAATAATAAGCAATTATAATTTTAGTCTTATCATATTTATAGGTTTATTCTCAAGTTGCTTATGAGATAATGGAGAAGAAATCGGCAGATGTGAAGCAAGGGATGTTTTCCAAAAGAAATCTTCTTCCTCGCCTGATTCTTCGAACACTTTACATGATTTTCTGTGGATTTCTAGCAGCTATGTTGCCGTTTTTTGGAGACATCAATGGCGTGGTTGGTGCTGTTGGTTTCATTCCTCTGGATTTTGTCCTGCCTATGATTTTATACAACAAAACACACAAGCCTCCAAAATCATCATTGACTTATTGGATCAACATTTCAATCATGGTGGCATTCACTGGTGCAGGAATCATGGGTGCATTCTCGTCCATAAGGAAATTAGTTCTTGATGCTAACCAATTTAAGCTGTTTGATAGTGAAGTTGTGGATTGA
Protein sequence

>MS.gene06058.t1

MPWQTHLRRIRITTLKMKMTPELLSSSSEWWHAGYHLTTAIVGPTILTLPYAFRGLGWGIGMLCLTVMGLVTFYSYCLMSKVLDHCENAGRRHIRFRELAADVLGSGWMSYFVIFIQTAINTGVGVGAILLAGQCLQIVYSNIAPHGALKLYDFIAMVTVVMIILSQLPTFHSLRHINLCSLFLSLGYTFLVVGACIHAGTSKNAPPRDYSLEPKESSRVFSAFTAISIIAAIFGNGILPEIQATLAPPATGKMIKGLTMCYAVILVTFYSAAVSGYWVFGNKSNSNILKSLMPDDGPSLAPTWVLGLAIVFVLLQLFAIGLVYSQVAYEIMEKKSADVKQGMFSKRNLLPRLILRTLYMIFCGFLAAMLPFFGDINGVVGAVGFIPLDFVLPMILYNKTHKPPKSSLTYWINISIMVAFTGAGIMGAFSSIRKLVLDANQFKLFDSEVVD