AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060600.t1 MTR_2g076590 80.000 440 81 4 1 435 1 438 0.0 703
MS.gene060600.t1 MTR_2g076490 79.224 438 68 4 1 435 1 418 0.0 697
MS.gene060600.t1 MTR_2g076610 70.470 298 64 3 89 384 19 294 6.80e-147 420
MS.gene060600.t1 MTR_2g076650 76.000 250 44 2 33 282 2 235 5.18e-134 385
MS.gene060600.t1 MTR_1g012550 25.840 387 233 16 63 432 65 414 7.59e-22 99.0
MS.gene060600.t1 MTR_2g089440 27.078 373 217 15 93 432 80 430 8.03e-21 95.9
MS.gene060600.t1 MTR_7g092050 28.338 367 213 19 60 409 48 381 1.50e-20 94.7
MS.gene060600.t1 MTR_3g031480 26.722 363 219 14 63 402 45 383 1.47e-19 92.0
MS.gene060600.t1 MTR_1g099400 23.923 418 258 18 60 432 33 435 2.03e-19 91.7
MS.gene060600.t1 MTR_7g058530 27.513 378 230 14 60 416 48 402 5.54e-19 90.1
MS.gene060600.t1 MTR_2g089360 25.886 367 218 15 93 428 77 420 7.44e-19 89.7
MS.gene060600.t1 MTR_7g056647 26.197 355 214 15 93 418 81 416 1.65e-18 88.6
MS.gene060600.t1 MTR_3g031640 26.331 357 229 13 63 403 42 380 2.09e-18 88.2
MS.gene060600.t1 MTR_3g031580 26.253 419 261 17 34 433 18 407 4.43e-18 87.4
MS.gene060600.t1 MTR_7g056663 23.669 338 206 13 93 400 75 390 9.03e-18 86.3
MS.gene060600.t1 MTR_7g058830 26.196 397 246 17 60 433 47 419 1.50e-17 85.9
MS.gene060600.t1 MTR_0280s0040 25.397 441 221 20 51 432 38 429 1.89e-17 85.5
MS.gene060600.t1 MTR_2g011160 25.556 360 223 13 63 400 48 384 2.08e-17 85.1
MS.gene060600.t1 MTR_2g011160 25.556 360 223 13 63 400 48 384 2.14e-17 85.1
MS.gene060600.t1 MTR_3g102400 25.474 369 213 16 60 397 45 382 3.45e-17 84.7
MS.gene060600.t1 MTR_7g072460 24.929 353 225 14 93 433 80 404 4.44e-17 83.6
MS.gene060600.t1 MTR_4g098890 22.353 340 212 13 93 402 652 969 4.92e-17 84.3
MS.gene060600.t1 MTR_3g031610 25.127 394 253 14 63 435 42 414 9.12e-17 83.2
MS.gene060600.t1 MTR_2g011150 24.654 361 225 14 63 400 50 386 1.33e-16 82.8
MS.gene060600.t1 MTR_7g056680 25.526 333 205 13 93 400 75 389 1.40e-16 82.8
MS.gene060600.t1 MTR_7g056680 25.526 333 205 13 93 400 75 389 1.40e-16 82.8
MS.gene060600.t1 MTR_7g056680 25.526 333 205 13 93 400 75 389 1.48e-16 82.8
MS.gene060600.t1 MTR_7g056680 25.526 333 205 13 93 400 75 389 1.48e-16 82.8
MS.gene060600.t1 MTR_3g031470 27.424 361 226 14 63 402 44 389 1.50e-16 82.8
MS.gene060600.t1 MTR_8g465510 25.600 375 224 14 57 400 38 388 1.57e-16 82.4
MS.gene060600.t1 MTR_2g011170 25.826 333 201 12 93 400 73 384 3.76e-16 81.3
MS.gene060600.t1 MTR_2g011170 25.826 333 201 12 93 400 73 384 4.15e-16 80.9
MS.gene060600.t1 MTR_7g056450 24.074 378 240 17 52 405 47 401 6.53e-16 80.5
MS.gene060600.t1 MTR_7g056450 24.074 378 240 17 52 405 37 391 9.13e-16 80.1
MS.gene060600.t1 MTR_2g011180 25.740 338 199 15 93 400 76 391 1.23e-15 79.7
MS.gene060600.t1 MTR_2g011180 25.740 338 199 15 93 400 76 391 1.29e-15 79.7
MS.gene060600.t1 MTR_2g073250 23.587 407 240 18 52 432 52 413 1.77e-15 79.3
MS.gene060600.t1 MTR_3g031500 26.111 360 211 15 96 433 2 328 7.38e-15 77.4
MS.gene060600.t1 MTR_8g013560 26.075 372 219 16 93 433 77 423 8.81e-15 77.0
MS.gene060600.t1 MTR_3g031490 26.382 398 240 18 63 433 42 413 1.64e-14 76.3
MS.gene060600.t1 MTR_5g067250 24.633 341 211 11 91 406 77 396 2.53e-14 75.9
MS.gene060600.t1 MTR_8g013610 24.533 375 222 14 93 433 78 425 3.70e-14 75.1
MS.gene060600.t1 MTR_5g067290 40.708 113 61 3 91 197 78 190 4.25e-14 74.3
MS.gene060600.t1 MTR_3g031600 25.915 355 231 12 63 402 42 379 7.01e-14 74.3
MS.gene060600.t1 MTR_7g056623 25.740 338 204 15 93 403 55 372 8.37e-14 73.9
MS.gene060600.t1 MTR_7g056420 23.932 351 209 19 90 405 410 737 1.82e-13 73.2
MS.gene060600.t1 MTR_7g056430 23.729 354 212 19 87 405 407 737 1.99e-13 73.2
MS.gene060600.t1 MTR_3g007650 24.528 477 269 23 1 432 5 435 5.57e-13 71.6
MS.gene060600.t1 MTR_7g056640 25.225 333 207 14 93 403 103 415 6.23e-13 71.2
MS.gene060600.t1 MTR_8g030500 23.367 398 262 15 60 433 31 409 6.45e-13 71.2
MS.gene060600.t1 MTR_6g043510 25.979 281 166 12 93 360 72 323 8.05e-13 70.9
MS.gene060600.t1 MTR_3g007630 23.709 426 236 18 60 432 45 434 8.10e-13 70.9
MS.gene060600.t1 MTR_3g020330 25.000 328 206 12 93 400 79 386 8.91e-13 70.9
MS.gene060600.t1 MTR_2g080080 22.841 359 213 17 93 430 90 405 1.12e-12 70.5
MS.gene060600.t1 MTR_3g019530 25.680 331 199 15 93 400 79 385 1.26e-12 70.1
MS.gene060600.t1 MTR_8g465580 24.523 367 235 15 93 434 74 423 1.34e-12 70.1
MS.gene060600.t1 MTR_3g019530 25.680 331 199 15 93 400 79 385 1.46e-12 70.1
MS.gene060600.t1 MTR_3g019530 25.680 331 199 15 93 400 79 385 1.60e-12 70.1
MS.gene060600.t1 MTR_8g465470 23.653 334 206 14 93 400 106 416 1.65e-12 70.1
MS.gene060600.t1 MTR_3g019530 25.680 331 199 15 93 400 79 385 1.65e-12 69.7
MS.gene060600.t1 MTR_3g019530 25.680 331 199 15 93 400 79 385 1.78e-12 69.7
MS.gene060600.t1 MTR_2g080100 22.556 399 239 18 60 432 61 415 2.31e-12 69.3
MS.gene060600.t1 MTR_2g103810 35.398 113 68 2 60 172 56 163 2.57e-12 69.3
MS.gene060600.t1 MTR_8g465410 25.341 367 232 15 93 434 74 423 2.88e-12 69.3
MS.gene060600.t1 MTR_3g051420 33.987 153 85 8 93 233 81 229 7.43e-12 67.8
MS.gene060600.t1 MTR_6g043790 25.758 264 144 10 93 339 77 305 7.88e-12 67.8
MS.gene060600.t1 MTR_8g465990 23.739 337 204 14 93 400 77 389 8.39e-12 67.4
MS.gene060600.t1 MTR_8g465990 23.739 337 204 14 93 400 77 389 1.01e-11 67.4
MS.gene060600.t1 MTR_8g465990 23.739 337 204 14 93 400 77 389 1.07e-11 67.4
MS.gene060600.t1 MTR_4g114240 23.364 428 286 19 1 402 1 412 1.43e-11 67.0
MS.gene060600.t1 MTR_2g089290 40.909 88 48 3 93 177 84 170 1.48e-11 67.0
MS.gene060600.t1 MTR_2g080090 22.404 366 217 17 93 432 90 414 1.55e-11 67.0
MS.gene060600.t1 MTR_1g052880 24.356 427 225 22 46 414 29 415 2.10e-11 66.6
MS.gene060600.t1 MTR_2g011190 28.968 252 138 14 93 328 80 306 2.12e-11 66.6
MS.gene060600.t1 MTR_2g011230 23.944 355 208 18 93 409 70 400 2.50e-11 66.2
MS.gene060600.t1 MTR_8g011410 24.654 361 194 17 93 410 80 405 5.09e-11 65.5
MS.gene060600.t1 MTR_8g051540 31.937 191 110 8 69 246 68 251 6.45e-11 65.1
MS.gene060600.t1 MTR_8g011430 24.148 352 210 12 93 410 85 413 9.82e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060600.t1 AT1G78860 46.528 432 213 8 15 434 9 434 7.13e-135 395
MS.gene060600.t1 AT1G78850 45.147 443 225 7 1 434 1 434 2.74e-134 393
MS.gene060600.t1 AT1G78830 42.544 456 222 11 10 431 5 454 1.04e-115 347
MS.gene060600.t1 AT1G78820 40.870 460 232 11 6 431 1 454 4.03e-111 335
MS.gene060600.t1 AT1G16905 41.429 420 212 7 28 434 24 422 4.98e-109 328
MS.gene060600.t1 AT4G00340 27.224 371 230 14 54 407 31 378 1.69e-22 100
MS.gene060600.t1 AT4G00340 27.224 371 230 14 54 407 31 378 1.90e-22 100
MS.gene060600.t1 AT5G24080 25.250 400 237 17 60 415 49 430 1.67e-21 97.8
MS.gene060600.t1 AT4G32300 24.021 383 213 13 60 411 52 387 4.90e-21 96.7
MS.gene060600.t1 AT5G35370 25.069 363 223 17 93 432 86 422 9.74e-20 92.4
MS.gene060600.t1 AT1G61380 26.549 339 198 15 93 402 70 386 3.86e-18 87.4
MS.gene060600.t1 AT4G11900 24.490 490 244 25 14 434 16 448 6.29e-18 86.7
MS.gene060600.t1 AT4G11900 24.490 490 244 25 14 434 16 448 6.29e-18 86.7
MS.gene060600.t1 AT4G11900 24.490 490 244 25 14 434 16 448 6.68e-18 86.7
MS.gene060600.t1 AT4G11900 24.490 490 244 25 14 434 16 448 6.68e-18 86.7
MS.gene060600.t1 AT4G11900 24.590 488 242 25 14 432 16 446 6.99e-18 86.7
MS.gene060600.t1 AT4G11900 24.590 488 242 25 14 432 16 446 6.99e-18 86.7
MS.gene060600.t1 AT4G11900 24.490 490 244 25 14 434 16 448 7.90e-18 86.7
MS.gene060600.t1 AT4G11900 24.490 490 244 25 14 434 16 448 1.02e-17 86.3
MS.gene060600.t1 AT4G11900 26.131 398 208 21 93 434 12 379 2.27e-17 85.1
MS.gene060600.t1 AT4G11900 26.131 398 208 21 93 434 12 379 3.20e-17 84.7
MS.gene060600.t1 AT3G12000 26.216 370 225 16 63 405 56 404 4.68e-17 83.2
MS.gene060600.t1 AT1G11330 24.927 341 209 13 93 404 84 406 1.19e-16 82.8
MS.gene060600.t1 AT1G11330 24.927 341 209 13 93 404 84 406 1.65e-16 82.4
MS.gene060600.t1 AT1G11330 24.927 341 209 13 93 404 84 406 1.65e-16 82.4
MS.gene060600.t1 AT4G03230 25.956 366 223 14 93 432 81 424 2.51e-16 82.0
MS.gene060600.t1 AT2G19130 23.096 407 262 19 51 430 34 416 2.75e-16 82.0
MS.gene060600.t1 AT4G03230 26.230 366 222 13 93 432 81 424 3.56e-16 81.6
MS.gene060600.t1 AT4G03230 26.230 366 222 13 93 432 81 424 3.56e-16 81.6
MS.gene060600.t1 AT1G65790 27.410 332 194 15 92 400 78 385 2.42e-15 78.6
MS.gene060600.t1 AT1G65790 27.410 332 194 15 92 400 78 385 2.42e-15 78.6
MS.gene060600.t1 AT1G65790 27.410 332 194 15 92 400 78 385 2.46e-15 79.0
MS.gene060600.t1 AT5G03700 25.360 347 186 17 113 432 109 409 2.99e-15 78.2
MS.gene060600.t1 AT1G61390 24.706 340 203 15 93 402 91 407 5.10e-15 77.8
MS.gene060600.t1 AT3G51710 21.739 345 220 10 102 434 103 409 1.82e-14 75.5
MS.gene060600.t1 AT1G61370 24.047 341 205 11 93 402 74 391 2.01e-14 75.9
MS.gene060600.t1 AT2G41890 26.899 316 195 12 103 405 91 383 5.40e-14 74.7
MS.gene060600.t1 AT1G61360 25.369 339 202 14 93 402 69 385 8.12e-14 74.3
MS.gene060600.t1 AT1G61610 25.729 377 218 18 60 400 48 398 3.28e-13 72.0
MS.gene060600.t1 AT1G61610 25.464 377 219 18 60 400 48 398 3.62e-13 72.0
MS.gene060600.t1 AT1G34300 25.606 289 173 12 60 342 46 298 1.37e-12 70.5
MS.gene060600.t1 AT4G27290 22.093 430 251 18 9 400 4 387 3.75e-12 68.9
MS.gene060600.t1 AT4G27290 22.093 430 251 18 9 400 4 387 4.10e-12 68.9
MS.gene060600.t1 AT1G11300 21.829 339 209 12 93 399 77 391 4.49e-12 68.6
MS.gene060600.t1 AT1G61420 25.882 340 193 16 93 402 73 383 4.75e-12 68.6
MS.gene060600.t1 AT1G61420 25.882 340 193 16 93 402 73 383 4.75e-12 68.6
MS.gene060600.t1 AT1G61420 25.882 340 193 16 93 402 73 383 4.87e-12 68.6
MS.gene060600.t1 AT1G11300 21.829 339 209 12 93 399 77 391 5.50e-12 68.6
MS.gene060600.t1 AT1G61480 24.702 336 202 16 93 402 73 383 9.68e-12 67.8
MS.gene060600.t1 AT1G11280 23.582 335 212 13 93 402 77 392 1.37e-11 67.0
MS.gene060600.t1 AT1G11280 23.582 335 212 13 93 402 77 392 1.38e-11 67.0
MS.gene060600.t1 AT1G11280 23.582 335 212 13 93 402 77 392 1.58e-11 67.0
MS.gene060600.t1 AT1G11280 23.582 335 212 13 93 402 87 402 1.69e-11 67.0
MS.gene060600.t1 AT1G11280 23.582 335 212 13 93 402 87 402 1.88e-11 66.6
MS.gene060600.t1 AT4G03230 25.217 345 211 12 113 432 30 352 2.11e-11 66.6

Find 71 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTTGATCAATATTTAA+AGG 0.189297 2.2:-17383224 MS.gene060600:CDS
CCTATTGGAATTCATCTAAT+TGG 0.210505 2.2:-17383072 MS.gene060600:CDS
CCAGAGAGATGTGGAAAATT+TGG 0.215504 2.2:-17382776 MS.gene060600:CDS
GATCAATATTTAAAGGCTAA+TGG 0.303903 2.2:-17383217 MS.gene060600:CDS
AAAGGCAACAACACCTATAT+TGG 0.309987 2.2:+17383333 None:intergenic
CAAATAAACACTTCCAATAT+AGG 0.327942 2.2:-17383346 MS.gene060600:CDS
ATATAGGTGTTGTTGCCTTT+AGG 0.351793 2.2:-17383330 MS.gene060600:CDS
GTTAAAGTGCTATTATTTAC+CGG 0.352398 2.2:+17382926 None:intergenic
TAATATAAAGTCAACCCTTT+GGG 0.365772 2.2:+17383124 None:intergenic
GGCTTATCTTATTACTTATT+AGG 0.367191 2.2:+17382431 None:intergenic
CCAATTAGATGAATTCCAAT+AGG 0.367562 2.2:+17383072 None:intergenic
GATGAATTCCAATAGGGAAT+TGG 0.367962 2.2:+17383079 None:intergenic
GGACCATTCATAGTAGAATA+TGG 0.369527 2.2:-17383601 MS.gene060600:CDS
CACCTAGGCCAATTCCCTAT+TGG 0.373218 2.2:-17383087 MS.gene060600:CDS
ATATCTGATCCATTTGAGAT+TGG 0.374078 2.2:-17383004 MS.gene060600:CDS
TCATCCTACAGATACTCTTT+TGG 0.375052 2.2:-17383242 MS.gene060600:CDS
ATAGCACTTTAACATATCTA+AGG 0.382513 2.2:-17382916 MS.gene060600:CDS
TGTTGATGGACCTTATAGTT+TGG 0.383460 2.2:-17383155 MS.gene060600:CDS
AGAGCTAGAGTGAAGGCATT+TGG 0.389629 2.2:+17383514 None:intergenic
GTAATATAAAGTCAACCCTT+TGG 0.401606 2.2:+17383123 None:intergenic
GACCTTCAAAACTTGTTAGT+AGG 0.402178 2.2:-17383195 MS.gene060600:CDS
GCCTTTAGGTTACTCTCAAA+TGG 0.414987 2.2:-17383316 MS.gene060600:CDS
TTTGTCAACTCAGGTGAACT+TGG 0.428871 2.2:-17383622 MS.gene060600:CDS
TATCTAAGGCTAGGAATTGA+TGG 0.429940 2.2:-17382902 MS.gene060600:CDS
TTTCATTACTACAAAATTGA+AGG 0.432875 2.2:-17382647 MS.gene060600:CDS
ACCTGAGTTGACAAATTTGA+AGG 0.439119 2.2:+17383630 None:intergenic
TTGATGTGCGTGATGGTGTT+TGG 0.440720 2.2:-17382853 MS.gene060600:CDS
AAGTGCAATCCAATCTCAAA+TGG 0.453322 2.2:+17382995 None:intergenic
GCCAACACACTTACAATCCT+TGG 0.454150 2.2:+17382553 None:intergenic
TGGAGAATGGAACATTTGCT+TGG 0.471864 2.2:-17382718 MS.gene060600:CDS
CCTCCATATTCTACTATGAA+TGG 0.498115 2.2:+17383598 None:intergenic
GAACAATAATGTGAGAAAAG+AGG 0.499200 2.2:+17383702 None:intergenic
CAATTAGATGAATTCCAATA+GGG 0.502032 2.2:+17383073 None:intergenic
TTCACTCTAGCTCTTCGAAT+GGG 0.502190 2.2:-17383505 MS.gene060600:CDS
TTCAATGCTCCATTCCAAGT+TGG 0.505787 2.2:-17383553 MS.gene060600:CDS
AACACCTTGAGTTATCTTTG+TGG 0.506678 2.2:+17382522 None:intergenic
ACCATTTGAGAGTAACCTAA+AGG 0.511899 2.2:+17383315 None:intergenic
ACCTTCAAATTTGTCAACTC+AGG 0.519002 2.2:-17383631 MS.gene060600:CDS
ATGCTGATGGAAGAATTGTT+TGG 0.528512 2.2:-17383369 MS.gene060600:CDS
CTTGTGTTGGCTAATGCTGA+TGG 0.532407 2.2:-17383382 MS.gene060600:CDS
ATGATCTTAATTGATACTAA+AGG 0.538143 2.2:-17383289 MS.gene060600:CDS
TATGAAAATATTTAGTGAGC+CGG 0.539381 2.2:-17382945 MS.gene060600:CDS
TACATGAGTAAATACACTAC+TGG 0.539734 2.2:-17382614 MS.gene060600:CDS
TTCCAATAGGGAATTGGCCT+AGG 0.540750 2.2:+17383085 None:intergenic
GTTGGTTGTCCATTGGAGAA+TGG 0.552362 2.2:-17382731 MS.gene060600:CDS
TTGTAGAAACCAACTTGGAA+TGG 0.552949 2.2:+17383544 None:intergenic
TCAATGTGTTGGTTGTCCAT+TGG 0.564083 2.2:-17382738 MS.gene060600:CDS
GATTGCATGAGAAATGAAAG+TGG 0.566775 2.2:+17383669 None:intergenic
ACAAAGATAACTCAAGGTGT+TGG 0.569098 2.2:-17382520 MS.gene060600:CDS
TATTTGCTTCCCAAACCCAC+CGG 0.574015 2.2:+17383458 None:intergenic
TGGAAAATTTGGACTTTGTG+AGG 0.578223 2.2:-17382765 MS.gene060600:CDS
AAACACCATCACGCACATCA+AGG 0.588769 2.2:+17382855 None:intergenic
ACTTTAACATATCTAAGGCT+AGG 0.589291 2.2:-17382911 MS.gene060600:CDS
TATTTCCTTGATGTGCGTGA+TGG 0.592648 2.2:-17382860 MS.gene060600:CDS
CTTAATAAAACCCACATGTG+TGG 0.606669 2.2:+17382457 None:intergenic
GCAAATGTTCCATTCTCCAA+TGG 0.608211 2.2:+17382722 None:intergenic
TGTGAGGATAGTCAATGTGT+TGG 0.608846 2.2:-17382749 MS.gene060600:CDS
CTTCACTCTAGCTCTTCGAA+TGG 0.609842 2.2:-17383506 MS.gene060600:CDS
AGTTCAAAATACCACACATG+TGG 0.615945 2.2:-17382468 MS.gene060600:CDS
TAGTGTTGTAGAAACCAACT+TGG 0.616231 2.2:+17383539 None:intergenic
TGGGTTTGGGAAGCAAATAG+AGG 0.619163 2.2:-17383454 MS.gene060600:CDS
TGTTGATGGAAATCTTGTGT+TGG 0.627952 2.2:-17383395 MS.gene060600:CDS
TCTACCACAAAGATAACTCA+AGG 0.628662 2.2:-17382526 MS.gene060600:CDS
TCGAATGGGACTTCAACGAT+CGG 0.632353 2.2:-17383491 MS.gene060600:CDS
ACCAAGGATTGTAAGTGTGT+TGG 0.640719 2.2:-17382554 MS.gene060600:CDS
TGTCAATTACCAGAGAGATG+TGG 0.650824 2.2:-17382785 MS.gene060600:CDS
TCAACCAAAAGAGTATCTGT+AGG 0.653995 2.2:+17383238 None:intergenic
TTGTGCAAATAAATGTACCA+AGG 0.661724 2.2:-17382570 MS.gene060600:CDS
GTTCAAAATACCACACATGT+GGG 0.676015 2.2:-17382467 MS.gene060600:CDS
CATTCGAAGAGCTAGAGTGA+AGG 0.676387 2.2:+17383507 None:intergenic
CCATTCATAGTAGAATATGG+AGG 0.699892 2.2:-17383598 MS.gene060600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATCTTAATTGATACTAA+AGG - chr2.2:17382880-17382899 MS.gene060600:CDS 20.0%
!! TTTCATTACTACAAAATTGA+AGG - chr2.2:17383522-17383541 MS.gene060600:CDS 20.0%
!! TTTGGTTGATCAATATTTAA+AGG - chr2.2:17382945-17382964 MS.gene060600:CDS 20.0%
!!! ATACTAAAGGTAATTTTGTT+TGG - chr2.2:17382893-17382912 MS.gene060600:CDS 20.0%
! ACAAAAGTAAAAATTCACCT+AGG - chr2.2:17383067-17383086 MS.gene060600:CDS 25.0%
! ATAGCACTTTAACATATCTA+AGG - chr2.2:17383253-17383272 MS.gene060600:CDS 25.0%
! CAAATAAACACTTCCAATAT+AGG - chr2.2:17382823-17382842 MS.gene060600:CDS 25.0%
! CAATTAGATGAATTCCAATA+GGG + chr2.2:17383099-17383118 None:intergenic 25.0%
! GATCAATATTTAAAGGCTAA+TGG - chr2.2:17382952-17382971 MS.gene060600:CDS 25.0%
! TAATATAAAGTCAACCCTTT+GGG + chr2.2:17383048-17383067 None:intergenic 25.0%
! TATGAAAATATTTAGTGAGC+CGG - chr2.2:17383224-17383243 MS.gene060600:CDS 25.0%
! TCAGAAAAAGAAAATGTTGA+TGG - chr2.2:17383000-17383019 MS.gene060600:CDS 25.0%
!! AATTTGAAGGTTTCATTTTG+TGG + chr2.2:17382529-17382548 None:intergenic 25.0%
!! GTTAAAGTGCTATTATTTAC+CGG + chr2.2:17383246-17383265 None:intergenic 25.0%
!!! GAAAATGCAACTTTTTCATT+AGG - chr2.2:17382751-17382770 MS.gene060600:CDS 25.0%
CCAATTAGATGAATTCCAAT+AGG + chr2.2:17383100-17383119 None:intergenic 30.0%
CCTATTGGAATTCATCTAAT+TGG - chr2.2:17383097-17383116 MS.gene060600:CDS 30.0%
GAACAATAATGTGAGAAAAG+AGG + chr2.2:17382470-17382489 None:intergenic 30.0%
GGATCAAAAACCAAACTATA+AGG + chr2.2:17383027-17383046 None:intergenic 30.0%
GTAATATAAAGTCAACCCTT+TGG + chr2.2:17383049-17383068 None:intergenic 30.0%
TACATGAGTAAATACACTAC+TGG - chr2.2:17383555-17383574 MS.gene060600:CDS 30.0%
TTGTGCAAATAAATGTACCA+AGG - chr2.2:17383599-17383618 MS.gene060600:CDS 30.0%
! AAAGTTGCATTTTCATCAAC+TGG + chr2.2:17382745-17382764 None:intergenic 30.0%
! AAATATTTTCATACCACCAG+AGG + chr2.2:17383217-17383236 None:intergenic 30.0%
! AAGTGTAATGCTAAGCTTTT+AGG - chr2.2:17383480-17383499 MS.gene060600:CDS 30.0%
! AAGTTGCATTTTCATCAACT+GGG + chr2.2:17382744-17382763 None:intergenic 30.0%
! ACTTTAACATATCTAAGGCT+AGG - chr2.2:17383258-17383277 MS.gene060600:CDS 30.0%
! ATATCTGATCCATTTGAGAT+TGG - chr2.2:17383165-17383184 MS.gene060600:CDS 30.0%
! ATGTGAGAAAAGAGGTTTTT+AGG + chr2.2:17382462-17382481 None:intergenic 30.0%
!!! ACTTTTTCATTAGGTGTTGA+TGG - chr2.2:17382760-17382779 MS.gene060600:CDS 30.0%
!!! TTTTTTCACAATTTTGCCTC+TGG - chr2.2:17383198-17383217 MS.gene060600:CDS 30.0%
AAAGGCAACAACACCTATAT+TGG + chr2.2:17382839-17382858 None:intergenic 35.0%
AACACCTTGAGTTATCTTTG+TGG + chr2.2:17383650-17383669 None:intergenic 35.0%
AAGTGCAATCCAATCTCAAA+TGG + chr2.2:17383177-17383196 None:intergenic 35.0%
ACAAAGATAACTCAAGGTGT+TGG - chr2.2:17383649-17383668 MS.gene060600:CDS 35.0%
ACCATTTGAGAGTAACCTAA+AGG + chr2.2:17382857-17382876 None:intergenic 35.0%
ACCTTCAAATTTGTCAACTC+AGG - chr2.2:17382538-17382557 MS.gene060600:CDS 35.0%
AGTTCAAAATACCACACATG+TGG - chr2.2:17383701-17383720 MS.gene060600:CDS 35.0%
ATGCTGATGGAAGAATTGTT+TGG - chr2.2:17382800-17382819 MS.gene060600:CDS 35.0%
CCATTCATAGTAGAATATGG+AGG - chr2.2:17382571-17382590 MS.gene060600:CDS 35.0%
CCTCCATATTCTACTATGAA+TGG + chr2.2:17382574-17382593 None:intergenic 35.0%
CTTAATAAAACCCACATGTG+TGG + chr2.2:17383715-17383734 None:intergenic 35.0%
GATGAATTCCAATAGGGAAT+TGG + chr2.2:17383093-17383112 None:intergenic 35.0%
GATTGCATGAGAAATGAAAG+TGG + chr2.2:17382503-17382522 None:intergenic 35.0%
GGACCATTCATAGTAGAATA+TGG - chr2.2:17382568-17382587 MS.gene060600:CDS 35.0%
GTTCAAAATACCACACATGT+GGG - chr2.2:17383702-17383721 MS.gene060600:CDS 35.0%
TAGTGTTGTAGAAACCAACT+TGG + chr2.2:17382633-17382652 None:intergenic 35.0%
TCTACCACAAAGATAACTCA+AGG - chr2.2:17383643-17383662 MS.gene060600:CDS 35.0%
TGGAAAATTTGGACTTTGTG+AGG - chr2.2:17383404-17383423 MS.gene060600:CDS 35.0%
TTGTAGAAACCAACTTGGAA+TGG + chr2.2:17382628-17382647 None:intergenic 35.0%
! ACCTGAGTTGACAAATTTGA+AGG + chr2.2:17382542-17382561 None:intergenic 35.0%
! TATCTAAGGCTAGGAATTGA+TGG - chr2.2:17383267-17383286 MS.gene060600:CDS 35.0%
! TCATCCTACAGATACTCTTT+TGG - chr2.2:17382927-17382946 MS.gene060600:CDS 35.0%
! TGTTGATGGAAATCTTGTGT+TGG - chr2.2:17382774-17382793 MS.gene060600:CDS 35.0%
! TGTTGATGGACCTTATAGTT+TGG - chr2.2:17383014-17383033 MS.gene060600:CDS 35.0%
!! AGCCTACTAACAAGTTTTGA+AGG + chr2.2:17382979-17382998 None:intergenic 35.0%
!! ATATAGGTGTTGTTGCCTTT+AGG - chr2.2:17382839-17382858 MS.gene060600:CDS 35.0%
!! GACCTTCAAAACTTGTTAGT+AGG - chr2.2:17382974-17382993 MS.gene060600:CDS 35.0%
!! TCAACCAAAAGAGTATCTGT+AGG + chr2.2:17382934-17382953 None:intergenic 35.0%
!!! AGTTTGGTTTTTGATCCCAA+AGG - chr2.2:17383030-17383049 MS.gene060600:CDS 35.0%
!!! CACACATGTGGGTTTTATTA+AGG - chr2.2:17383713-17383732 MS.gene060600:CDS 35.0%
!!! GTTTGGTTTTTGATCCCAAA+GGG - chr2.2:17383031-17383050 MS.gene060600:CDS 35.0%
ACCAAGGATTGTAAGTGTGT+TGG - chr2.2:17383615-17383634 MS.gene060600:CDS 40.0%
CCAGAGAGATGTGGAAAATT+TGG - chr2.2:17383393-17383412 MS.gene060600:CDS 40.0%
GCAAATGTTCCATTCTCCAA+TGG + chr2.2:17383450-17383469 None:intergenic 40.0%
TATTTCCTTGATGTGCGTGA+TGG - chr2.2:17383309-17383328 MS.gene060600:CDS 40.0%
TGGAGAATGGAACATTTGCT+TGG - chr2.2:17383451-17383470 MS.gene060600:CDS 40.0%
TGTCAATTACCAGAGAGATG+TGG - chr2.2:17383384-17383403 MS.gene060600:CDS 40.0%
TGTGAGGATAGTCAATGTGT+TGG - chr2.2:17383420-17383439 MS.gene060600:CDS 40.0%
TTCAATGCTCCATTCCAAGT+TGG - chr2.2:17382616-17382635 MS.gene060600:CDS 40.0%
TTTGTCAACTCAGGTGAACT+TGG - chr2.2:17382547-17382566 MS.gene060600:CDS 40.0%
! CCAAATTTTCCACATCTCTC+TGG + chr2.2:17383396-17383415 None:intergenic 40.0%
! GCCTTTAGGTTACTCTCAAA+TGG - chr2.2:17382853-17382872 MS.gene060600:CDS 40.0%
! TTTCACAATTTTGCCTCTGG+TGG - chr2.2:17383201-17383220 MS.gene060600:CDS 40.0%
!! TCAATGTGTTGGTTGTCCAT+TGG - chr2.2:17383431-17383450 MS.gene060600:CDS 40.0%
!! TTCACTCTAGCTCTTCGAAT+GGG - chr2.2:17382664-17382683 MS.gene060600:CDS 40.0%
AAACACCATCACGCACATCA+AGG + chr2.2:17383317-17383336 None:intergenic 45.0%
CATTCGAAGAGCTAGAGTGA+AGG + chr2.2:17382665-17382684 None:intergenic 45.0%
GCCAACACACTTACAATCCT+TGG + chr2.2:17383619-17383638 None:intergenic 45.0%
GTTGGTTGTCCATTGGAGAA+TGG - chr2.2:17383438-17383457 MS.gene060600:CDS 45.0%
TATTTGCTTCCCAAACCCAC+CGG + chr2.2:17382714-17382733 None:intergenic 45.0%
TCGAATGGGACTTCAACGAT+CGG - chr2.2:17382678-17382697 MS.gene060600:CDS 45.0%
TGGGTTTGGGAAGCAAATAG+AGG - chr2.2:17382715-17382734 MS.gene060600:CDS 45.0%
! AACGATCGGAGCAGTTTTTC+CGG - chr2.2:17382692-17382711 MS.gene060600:CDS 45.0%
! TTCCAATAGGGAATTGGCCT+AGG + chr2.2:17383087-17383106 None:intergenic 45.0%
! TTGATGTGCGTGATGGTGTT+TGG - chr2.2:17383316-17383335 MS.gene060600:CDS 45.0%
!! AGAGCTAGAGTGAAGGCATT+TGG + chr2.2:17382658-17382677 None:intergenic 45.0%
!! CTTCACTCTAGCTCTTCGAA+TGG - chr2.2:17382663-17382682 MS.gene060600:CDS 45.0%
!! CTTGTGTTGGCTAATGCTGA+TGG - chr2.2:17382787-17382806 MS.gene060600:CDS 45.0%
CACCTAGGCCAATTCCCTAT+TGG - chr2.2:17383082-17383101 MS.gene060600:CDS 50.0%
! ATCGGAGCAGTTTTTCCGGT+GGG - chr2.2:17382696-17382715 MS.gene060600:CDS 50.0%
!! AGCAGTTTTTCCGGTGGGTT+TGG - chr2.2:17382701-17382720 MS.gene060600:CDS 50.0%
!! GCAGTTTTTCCGGTGGGTTT+GGG - chr2.2:17382702-17382721 MS.gene060600:CDS 50.0%
! GATCGGAGCAGTTTTTCCGG+TGG - chr2.2:17382695-17382714 MS.gene060600:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 17382442 17383749 17382442 ID=MS.gene060600
chr2.2 mRNA 17382442 17383749 17382442 ID=MS.gene060600.t1;Parent=MS.gene060600
chr2.2 exon 17382442 17383749 17382442 ID=MS.gene060600.t1.exon1;Parent=MS.gene060600.t1
chr2.2 CDS 17382442 17383749 17382442 ID=cds.MS.gene060600.t1;Parent=MS.gene060600.t1
Gene Sequence

>MS.gene060600

ATGTCTTTTGTTCTTCTCCTAAAAACCTCTTTTCTCACATTATTGTTCTTATCATTCTCCACTTTCATTTCTCATGCAATCGTACCACAAAATGAAACCTTCAAATTTGTCAACTCAGGTGAACTTGGACCATTCATAGTAGAATATGGAGGTGATTACAGAATGATAAGCATATTCAATGCTCCATTCCAAGTTGGTTTCTACAACACTACTCCAAATGCCTTCACTCTAGCTCTTCGAATGGGACTTCAACGATCGGAGCAGTTTTTCCGGTGGGTTTGGGAAGCAAATAGAGGCAACCCAGTTGATGAAAATGCAACTTTTTCATTAGGTGTTGATGGAAATCTTGTGTTGGCTAATGCTGATGGAAGAATTGTTTGGCAAATAAACACTTCCAATATAGGTGTTGTTGCCTTTAGGTTACTCTCAAATGGTAACATGATCTTAATTGATACTAAAGGTAATTTTGTTTGGCAAAGTTTTGATCATCCTACAGATACTCTTTTGGTTGATCAATATTTAAAGGCTAATGGACCTTCAAAACTTGTTAGTAGGCTTTCAGAAAAAGAAAATGTTGATGGACCTTATAGTTTGGTTTTTGATCCCAAAGGGTTGACTTTATATTACAAAAGTAAAAATTCACCTAGGCCAATTCCCTATTGGAATTCATCTAATTGGTATTCATTTGATTTTAAACAATTTACATTAGAAAAAGTCACACTCATATCTGATCCATTTGAGATTGGATTGCACTTGTTTTTTCACAATTTTGCCTCTGGTGGTATGAAAATATTTAGTGAGCCGGTAAATAATAGCACTTTAACATATCTAAGGCTAGGAATTGATGGTAACATTAAAATTCACACTTATTTCCTTGATGTGCGTGATGGTGTTTGGCAAGTGATGTACACTCTCTTTGATAGAAATTTTGATGAGAGTGAGTGTCAATTACCAGAGAGATGTGGAAAATTTGGACTTTGTGAGGATAGTCAATGTGTTGGTTGTCCATTGGAGAATGGAACATTTGCTTGGAGCAATAAGTGTAATGCTAAGCTTTTAGGAGAATGCAAAACAAGTGAGTTTCATTACTACAAAATTGAAGGAGTTGAGCATTACATGAGTAAATACACTACTGGTGATAGAGTAAGTGAAGATACTTGTGCAAATAAATGTACCAAGGATTGTAAGTGTGTTGGCTATTTCTACCACAAAGATAACTCAAGGTGTTGGATTGCTTATGATCTTCAAACTCTTACTAGAGTTCAAAATACCACACATGTGGGTTTTATTAAGGTGCCTAATAAGTAA
Protein sequence

>MS.gene060600.t1

MSFVLLLKTSFLTLLFLSFSTFISHAIVPQNETFKFVNSGELGPFIVEYGGDYRMISIFNAPFQVGFYNTTPNAFTLALRMGLQRSEQFFRWVWEANRGNPVDENATFSLGVDGNLVLANADGRIVWQINTSNIGVVAFRLLSNGNMILIDTKGNFVWQSFDHPTDTLLVDQYLKANGPSKLVSRLSEKENVDGPYSLVFDPKGLTLYYKSKNSPRPIPYWNSSNWYSFDFKQFTLEKVTLISDPFEIGLHLFFHNFASGGMKIFSEPVNNSTLTYLRLGIDGNIKIHTYFLDVRDGVWQVMYTLFDRNFDESECQLPERCGKFGLCEDSQCVGCPLENGTFAWSNKCNAKLLGECKTSEFHYYKIEGVEHYMSKYTTGDRVSEDTCANKCTKDCKCVGYFYHKDNSRCWIAYDLQTLTRVQNTTHVGFIKVPNK