AlfalfaGEDB | MS.gene061013

M. sativa cultivar XinJiangDaYe / MS.gene061013

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061013.t1	XP_024631467.1	97.1	170	5	0	13	182	129	298	4.60E-85	323.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061013.t1	Q9FNN6	60.7	178	58	4	13	182	125	298	1.3e-40	167.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061013.t1	A0A396JPI4	97.1	170	5	0	13	182	129	298	3.4e-85	323.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene061013.t1	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene061013	MS.gene071069	0.990397	1.94E-182	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061013.t1	MTR_1g033620	95.455	176	8	0	7	182	6	181	3.27e-120	340
MS.gene061013.t1	MTR_8g063600	62.644	174	53	4	13	175	113	285	4.41e-63	196
MS.gene061013.t1	MTR_4g111975	67.407	135	36	4	13	140	145	278	1.75e-49	162
MS.gene061013.t1	MTR_8g063870	45.455	187	73	4	5	173	91	266	1.66e-41	140
MS.gene061013.t1	MTR_1g111830	73.913	69	18	0	13	81	131	199	5.74e-30	110
MS.gene061013.t1	MTR_3g462790	78.788	66	14	0	14	79	155	220	1.21e-29	111
MS.gene061013.t1	MTR_5g069710	80.645	62	12	0	13	74	139	200	6.02e-29	109
MS.gene061013.t1	MTR_7g067080	76.471	68	16	0	14	81	148	215	1.46e-28	108
MS.gene061013.t1	MTR_4g015130	72.881	59	16	0	16	74	3	61	9.14e-26	94.7
MS.gene061013.t1	MTR_5g081860	69.014	71	20	1	13	81	132	202	9.17e-26	100
MS.gene061013.t1	MTR_6g092540	57.955	88	30	2	14	101	105	185	1.04e-24	97.8
MS.gene061013.t1	MTR_4g100630	61.194	67	26	0	13	79	102	168	2.67e-23	94.4
MS.gene061013.t1	MTR_8g101650	70.175	57	16	1	14	70	113	168	1.29e-22	92.0
MS.gene061013.t1	MTR_2g100930	51.020	98	40	1	13	102	116	213	2.58e-22	92.0
MS.gene061013.t1	MTR_5g027570	55.714	70	29	1	13	80	99	168	1.60e-20	85.5
MS.gene061013.t1	MTR_0036s0260	63.333	60	22	0	13	72	35	94	2.60e-20	84.3
MS.gene061013.t1	MTR_5g088010	58.209	67	24	2	14	77	68	133	2.83e-20	85.1
MS.gene061013.t1	MTR_5g088060	58.209	67	24	2	14	77	280	345	2.28e-19	84.7
MS.gene061013.t1	MTR_5g488160	58.209	67	24	2	14	77	280	345	2.28e-19	84.7
MS.gene061013.t1	MTR_5g037080	71.667	60	17	0	13	72	109	168	1.65e-18	81.3
MS.gene061013.t1	MTR_2g090305	61.667	60	23	0	13	72	35	94	2.18e-18	79.0
MS.gene061013.t1	MTR_4g107230	82.051	39	7	0	14	52	92	130	7.61e-16	73.9
MS.gene061013.t1	MTR_3g104370	57.627	59	22	2	15	70	100	158	9.06e-16	72.8
MS.gene061013.t1	MTR_5g027550	52.113	71	31	1	13	80	84	154	1.66e-15	72.0
MS.gene061013.t1	MTR_1g048660	46.875	64	34	0	14	77	20	83	6.08e-14	64.7
MS.gene061013.t1	MTR_1g083180	50.746	67	29	2	13	76	68	133	4.09e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061013.t1	AT5G08520	66.304	184	38	5	13	182	125	298	3.62e-69	212
MS.gene061013.t1	AT1G49010	56.618	136	54	2	13	143	141	276	2.24e-40	139
MS.gene061013.t1	AT5G23650	39.024	205	79	3	13	171	127	331	7.86e-34	122
MS.gene061013.t1	AT5G04760	77.419	62	14	0	13	74	106	167	1.78e-29	108
MS.gene061013.t1	AT5G58900	80.328	61	12	0	14	74	149	209	3.40e-29	109
MS.gene061013.t1	AT2G38090	78.689	61	13	0	14	74	149	209	1.27e-27	105
MS.gene061013.t1	AT5G01200	74.576	59	15	0	16	74	156	214	2.66e-25	99.0
MS.gene061013.t1	AT5G05790	69.014	71	20	1	13	81	138	208	1.63e-24	97.1
MS.gene061013.t1	AT5G05790	69.014	71	20	1	13	81	138	208	1.63e-24	97.1
MS.gene061013.t1	AT3G11280	74.194	62	16	0	13	74	134	195	6.80e-24	95.1
MS.gene061013.t1	AT3G11280	74.194	62	16	0	13	74	134	195	6.80e-24	95.1
MS.gene061013.t1	AT5G61620	57.647	85	26	2	13	87	115	199	2.92e-23	94.4
MS.gene061013.t1	AT5G47390	63.636	66	24	0	13	78	103	168	3.03e-23	95.1
MS.gene061013.t1	AT5G56840	53.659	82	37	1	13	93	98	179	1.78e-22	90.5
MS.gene061013.t1	AT3G10580	59.420	69	26	1	13	81	102	168	7.58e-20	84.0
MS.gene061013.t1	AT3G10580	59.420	69	26	1	13	81	102	168	1.27e-19	84.0
MS.gene061013.t1	AT1G70000	75.862	58	14	0	14	71	106	163	1.83e-18	80.5
MS.gene061013.t1	AT1G70000	75.862	58	14	0	14	71	106	163	1.83e-18	80.5
MS.gene061013.t1	AT4G09450	61.017	59	23	0	13	71	98	156	4.82e-18	78.2
MS.gene061013.t1	AT3G16350	80.488	41	8	0	13	53	144	184	9.16e-17	77.0
MS.gene061013.t1	AT1G19000	51.485	101	43	2	14	109	110	209	1.78e-16	75.5
MS.gene061013.t1	AT1G19000	51.485	101	43	2	14	109	110	209	1.78e-16	75.5
MS.gene061013.t1	AT1G74840	77.500	40	9	0	13	52	103	142	3.53e-15	71.2
MS.gene061013.t1	AT1G74840	77.500	40	9	0	13	52	103	142	5.28e-15	71.2
MS.gene061013.t1	AT3G10590	58.621	58	23	1	14	71	119	175	1.78e-12	63.5

Find 0 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTCATATTTTGCTTCAAAT+TGG	+	6873:15500-15519	MS.gene061013:CDS	25.0%
!!	TTCAACAGGTTATTTCTTCT+GGG	+	6873:15523-15542	MS.gene061013:CDS	30.0%
	GACATTACAAGCGTTAACAA+CGG	+	6873:15694-15713	MS.gene061013:CDS	35.0%
!	TTGCTTCAAATTGGTTCAAC+AGG	+	6873:15509-15528	MS.gene061013:CDS	35.0%
!!	GTTCAACAGGTTATTTCTTC+TGG	+	6873:15522-15541	MS.gene061013:CDS	35.0%
	ACTCGATGAACAAAGACAGA+AGG	+	6873:15656-15675	MS.gene061013:CDS	40.0%
	GATACTAAAGGTCCTCCTAT+CGG	-	6873:15865-15884	MS.gene061013:intergenic	40.0%
!	CAGAAGTATGATGAGGCATA+GGG	-	6873:16005-16024	MS.gene061013:intergenic	40.0%
!	TAGCTACTTGTGTTGGTGTT+CGG	-	6873:15606-15625	MS.gene061013:intergenic	40.0%
	AGATGAACCGTTCGTTTGAC+CGG	-	6873:15749-15768	MS.gene061013:intergenic	45.0%
	ATACCATAAGCCATGTGTGG+AGG	-	6873:15919-15938	MS.gene061013:intergenic	45.0%
	ATCATACTTCTGCTCCTCAC+AGG	+	6873:16013-16032	MS.gene061013:CDS	45.0%
	CGAATACCATAAGCCATGTG+TGG	-	6873:15922-15941	MS.gene061013:intergenic	45.0%
	GAGGCATAGGGTATGTCATA+GGG	-	6873:15993-16012	MS.gene061013:intergenic	45.0%
	GGACCTTTAGTATCTGCTGT+TGG	+	6873:15871-15890	MS.gene061013:CDS	45.0%
	GGTTCATCTGGAAATTCTGC+TGG	+	6873:15760-15779	MS.gene061013:CDS	45.0%
	GTGCCAACAGCAGATACTAA+AGG	-	6873:15877-15896	MS.gene061013:intergenic	45.0%
	TGAGGCATAGGGTATGTCAT+AGG	-	6873:15994-16013	MS.gene061013:intergenic	45.0%
!	AGCTACTTGTGTTGGTGTTC+GGG	-	6873:15605-15624	MS.gene061013:intergenic	45.0%
!	CTATCGGTTGTCCAATGGTA+GGG	-	6873:15849-15868	MS.gene061013:intergenic	45.0%
!	GCAGAAGTATGATGAGGCAT+AGG	-	6873:16006-16025	MS.gene061013:intergenic	45.0%
!	GCATGACTAGCTACTTGTGT+TGG	-	6873:15613-15632	MS.gene061013:intergenic	45.0%
!	TATCGGTTGTCCAATGGTAG+GGG	-	6873:15848-15867	MS.gene061013:intergenic	45.0%
!	TGAGGAGCAGAAGTATGATG+AGG	-	6873:16012-16031	MS.gene061013:intergenic	45.0%
	AAGCTGTTCCATCGACAACC+GGG	+	6873:15797-15816	MS.gene061013:CDS	50.0%
	ACAAGTACGGAAAAGGCGAC+TGG	+	6873:15551-15570	MS.gene061013:CDS	50.0%
	ACCATTGGACAACCGATAGG+AGG	+	6873:15850-15869	MS.gene061013:CDS	50.0%
	AGGAGCGGAAAGATTCACAG+GGG	-	6873:15899-15918	MS.gene061013:intergenic	50.0%
	ATAAGCCATGTGTGGAGGAG+CGG	-	6873:15914-15933	MS.gene061013:intergenic	50.0%
	ATGTATGCTGCCCCTACCAT+TGG	+	6873:15835-15854	MS.gene061013:CDS	50.0%
	CAAGCTGTTCCATCGACAAC+CGG	+	6873:15796-15815	MS.gene061013:CDS	50.0%
	CCTACCATTGGACAACCGAT+AGG	+	6873:15847-15866	MS.gene061013:CDS	50.0%
	GAGGAGCGGAAAGATTCACA+GGG	-	6873:15900-15919	MS.gene061013:intergenic	50.0%
	GGAGATGTTTCAACGCCTCA+AGG	+	6873:15715-15734	MS.gene061013:CDS	50.0%
	GGTCAAACGAACGGTTCATC+TGG	+	6873:15748-15767	MS.gene061013:CDS	50.0%
	GTCATAGGGACCATGTTCAC+TGG	-	6873:15979-15998	MS.gene061013:intergenic	50.0%
!	CCTATCGGTTGTCCAATGGT+AGG	-	6873:15850-15869	MS.gene061013:intergenic	50.0%
!	TCCTCCTATCGGTTGTCCAA+TGG	-	6873:15854-15873	MS.gene061013:intergenic	50.0%
!!	CAGGTTATTTCTTCTGGGCC+TGG	+	6873:15528-15547	MS.gene061013:CDS	50.0%
	ACCGTTCGTTTGACCGGTGA+TGG	-	6873:15743-15762	MS.gene061013:intergenic	55.0%
	AGCTGTTCCATCGACAACCG+GGG	+	6873:15798-15817	MS.gene061013:CDS	55.0%
	CCCATCACCGGTCAAACGAA+CGG	+	6873:15739-15758	MS.gene061013:CDS	55.0%
	CCGTTCGTTTGACCGGTGAT+GGG	-	6873:15742-15761	MS.gene061013:intergenic	55.0%
	CTTCTGGGCCTGGACAAGTA+CGG	+	6873:15538-15557	MS.gene061013:CDS	55.0%
	GACACAACTGTACCGGGAAC+TGG	-	6873:15949-15968	MS.gene061013:intergenic	55.0%
	GACCATGTTCACTGGTGCAC+CGG	-	6873:15971-15990	MS.gene061013:intergenic	55.0%
	GCAGCATACATTCCTGCACC+TGG	-	6873:15826-15845	MS.gene061013:intergenic	55.0%
	GCTCCTCCACACATGGCTTA+TGG	+	6873:15913-15932	MS.gene061013:CDS	55.0%
	GGAGGAGCGGAAAGATTCAC+AGG	-	6873:15901-15920	MS.gene061013:intergenic	55.0%
	GGCCTGGACAAGTACGGAAA+AGG	+	6873:15544-15563	MS.gene061013:CDS	55.0%
	GGTATTCGAGCACCAGTTCC+CGG	+	6873:15934-15953	MS.gene061013:CDS	55.0%
	GTTCCCGGTACAGTTGTGTC+CGG	+	6873:15949-15968	MS.gene061013:CDS	55.0%
	TCTTTCCGCTCCTCCACACA+TGG	+	6873:15906-15925	MS.gene061013:CDS	55.0%
	TGCACCGGACACAACTGTAC+CGG	-	6873:15956-15975	MS.gene061013:intergenic	55.0%
!	CGCCTTTTCCGTACTTGTCC+AGG	-	6873:15549-15568	MS.gene061013:intergenic	55.0%
	ACGCCTCAAGGACCCATCAC+CGG	+	6873:15727-15746	MS.gene061013:CDS	60.0%
	CATTCCTGCACCTGGTACCC+CGG	-	6873:15818-15837	MS.gene061013:intergenic	60.0%
	GCACCGGACACAACTGTACC+GGG	-	6873:15955-15974	MS.gene061013:intergenic	60.0%
	GTCCGGTGCACCAGTGAACA+TGG	+	6873:15966-15985	MS.gene061013:CDS	60.0%
!!	TGACCGGTGATGGGTCCTTG+AGG	-	6873:15733-15752	MS.gene061013:intergenic	60.0%
	CCTGGTACCCCGGTTGTCGA+TGG	-	6873:15808-15827	MS.gene061013:intergenic	65.0%
!	ACAACCGGGGTACCAGGTGC+AGG	+	6873:15811-15830	MS.gene061013:CDS	65.0%
!	CCATCGACAACCGGGGTACC+AGG	+	6873:15805-15824	MS.gene061013:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
6873	gene	15490	16038	15490	ID=MS.gene061013
6873	mRNA	15490	16038	15490	ID=MS.gene061013.t1;Parent=MS.gene061013
6873	exon	15490	16038	15490	ID=MS.gene061013.t1.exon1;Parent=MS.gene061013.t1
6873	CDS	15490	16038	15490	ID=cds.MS.gene061013.t1;Parent=MS.gene061013.t1

Gene Sequence

>MS.gene061013

ATGAAATGGTTCTCATATTTTGCTTCAAATTGGTTCAACAGGTTATTTCTTCTGGGCCTGGACAAGTACGGAAAAGGCGACTGGCGAAGCATTTCAAGAAACTTTGTTGTAACCCGAACACCAACACAAGTAGCTAGTCATGCACAAAAGTACTTCATTCGACTGAACTCGATGAACAAAGACAGAAGGCGATCGAGCATACATGACATTACAAGCGTTAACAACGGAGATGTTTCAACGCCTCAAGGACCCATCACCGGTCAAACGAACGGTTCATCTGGAAATTCTGCTGGCAAATCAGTCAAACAAGCTGTTCCATCGACAACCGGGGTACCAGGTGCAGGAATGTATGCTGCCCCTACCATTGGACAACCGATAGGAGGACCTTTAGTATCTGCTGTTGGCACCCCTGTGAATCTTTCCGCTCCTCCACACATGGCTTATGGTATTCGAGCACCAGTTCCCGGTACAGTTGTGTCCGGTGCACCAGTGAACATGGTCCCTATGACATACCCTATGCCTCATCATACTTCTGCTCCTCACAGGTAA

Protein sequence

>MS.gene061013.t1

MKWFSYFASNWFNRLFLLGLDKYGKGDWRSISRNFVVTRTPTQVASHAQKYFIRLNSMNKDRRRSSIHDITSVNNGDVSTPQGPITGQTNGSSGNSAGKSVKQAVPSTTGVPGAGMYAAPTIGQPIGGPLVSAVGTPVNLSAPPHMAYGIRAPVPGTVVSGAPVNMVPMTYPMPHHTSAPHR