Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061248.t1 | XP_003609812.1 | 94.4 | 177 | 6 | 1 | 1 | 177 | 1 | 173 | 2.20E-84 | 321.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061248.t1 | F4HWQ8 | 47.5 | 141 | 74 | 0 | 32 | 172 | 21 | 161 | 5.0e-32 | 139.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061248.t1 | G7JP02 | 94.4 | 177 | 6 | 1 | 1 | 177 | 1 | 173 | 1.6e-84 | 321.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061248 | MS.gene49486 | 0.804637 | 1.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061248.t1 | MTR_4g122750 | 94.350 | 177 | 6 | 1 | 1 | 177 | 1 | 173 | 1.45e-119 | 335 |
| MS.gene061248.t1 | MTR_4g122760 | 72.222 | 180 | 43 | 3 | 1 | 177 | 1 | 176 | 3.43e-85 | 248 |
| MS.gene061248.t1 | MTR_4g122890 | 54.190 | 179 | 76 | 4 | 1 | 177 | 1 | 175 | 1.04e-60 | 186 |
| MS.gene061248.t1 | MTR_4g122800 | 52.198 | 182 | 82 | 1 | 1 | 177 | 1 | 182 | 1.71e-59 | 183 |
| MS.gene061248.t1 | MTR_4g007280 | 52.778 | 108 | 49 | 2 | 72 | 177 | 1 | 108 | 1.94e-28 | 102 |
| MS.gene061248.t1 | MTR_4g122780 | 41.722 | 151 | 72 | 3 | 43 | 177 | 8 | 158 | 1.36e-25 | 96.7 |
| MS.gene061248.t1 | MTR_2g033630 | 36.257 | 171 | 97 | 4 | 19 | 177 | 6 | 176 | 7.76e-22 | 87.4 |
| MS.gene061248.t1 | MTR_4g122740 | 35.443 | 158 | 97 | 3 | 25 | 177 | 20 | 177 | 1.50e-19 | 81.6 |
| MS.gene061248.t1 | MTR_4g122660 | 28.729 | 181 | 117 | 5 | 3 | 177 | 2 | 176 | 6.85e-18 | 77.4 |
| MS.gene061248.t1 | MTR_4g122720 | 31.293 | 147 | 97 | 2 | 35 | 177 | 39 | 185 | 5.46e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061248.t1 | AT1G47960 | 47.945 | 146 | 76 | 0 | 32 | 177 | 21 | 166 | 1.02e-46 | 150 |
| MS.gene061248.t1 | AT1G47960 | 47.183 | 142 | 75 | 0 | 32 | 173 | 21 | 162 | 1.11e-43 | 144 |
| MS.gene061248.t1 | AT3G17152 | 30.857 | 175 | 119 | 1 | 4 | 176 | 1 | 175 | 8.88e-18 | 76.6 |
| MS.gene061248.t1 | AT3G17150 | 31.469 | 143 | 96 | 1 | 37 | 177 | 39 | 181 | 2.25e-15 | 70.5 |
| MS.gene061248.t1 | AT3G17140 | 37.895 | 95 | 57 | 2 | 84 | 177 | 19 | 112 | 9.59e-15 | 67.4 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATGCAATTTGCATAATT+TGG | 0.146977 | 8.3:-6026723 | None:intergenic |
| GCACCATCTTCTGCAAATTT+AGG | 0.160091 | 8.3:-6026972 | None:intergenic |
| ATCAAATTAGCATTGGATTT+TGG | 0.186303 | 8.3:-6026678 | None:intergenic |
| TTGAGAGCTGCAACAGATTT+TGG | 0.208499 | 8.3:-6026939 | None:intergenic |
| CAAAATCAATCAGTTGATTA+AGG | 0.217105 | 8.3:+6026845 | MS.gene061248:CDS |
| ACATCTGCATCAGAGCTTCT+TGG | 0.293970 | 8.3:-6026762 | None:intergenic |
| TGGTAGATACAAAGCAATTT+TGG | 0.313317 | 8.3:+6026905 | MS.gene061248:CDS |
| AATGCTAATGCTAATGCTAA+TGG | 0.350974 | 8.3:-6026606 | None:intergenic |
| GTTTAAGGCAGTGTTTGCTT+TGG | 0.395895 | 8.3:-6026823 | None:intergenic |
| AAAATCAATCAGTTGATTAA+GGG | 0.419264 | 8.3:+6026846 | MS.gene061248:CDS |
| AGCTCTGATGCAGATGTAAC+AGG | 0.425806 | 8.3:+6026768 | MS.gene061248:CDS |
| ACTTGTGAGAATGGCTTCAA+TGG | 0.435357 | 8.3:+6027020 | MS.gene061248:CDS |
| AGCTTCTTGGATCAGATTTG+AGG | 0.439581 | 8.3:-6026749 | None:intergenic |
| GAAGCCACTACTTGTGAGAA+TGG | 0.448649 | 8.3:+6027011 | MS.gene061248:CDS |
| TTGCTGTATCAAATTAGCAT+TGG | 0.473617 | 8.3:-6026685 | None:intergenic |
| TAAGGGGAGCCATGATCAAA+AGG | 0.485227 | 8.3:+6026863 | MS.gene061248:CDS |
| CTTGTGAGAATGGCTTCAAT+GGG | 0.531153 | 8.3:+6027021 | MS.gene061248:CDS |
| GATCCTAAATTTGCAGAAGA+TGG | 0.542535 | 8.3:+6026969 | MS.gene061248:CDS |
| GAAGCTTTGAATTCATGTGC+TGG | 0.550109 | 8.3:+6026885 | MS.gene061248:CDS |
| AACAGGTCTAGCACTAATCA+TGG | 0.552410 | 8.3:+6026785 | MS.gene061248:CDS |
| GAAGCCATTCTCACAAGTAG+TGG | 0.571548 | 8.3:-6027015 | None:intergenic |
| AAATCAATCAGTTGATTAAG+GGG | 0.571558 | 8.3:+6026847 | MS.gene061248:CDS |
| TCTGTTGCAGCTCTCAAGCA+AGG | 0.585277 | 8.3:+6026945 | MS.gene061248:CDS |
| GCAATATTCTCATCAGAGAG+TGG | 0.649907 | 8.3:-6027050 | None:intergenic |
| AGGTCTAGCACTAATCATGG+TGG | 0.723735 | 8.3:+6026788 | MS.gene061248:CDS |
| TGATGTAGCAGTCATAACAG+CGG | 0.751169 | 8.3:+6027079 | MS.gene061248:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCAATCAGTTGATTAA+GGG | + | chr8.3:6026846-6026865 | MS.gene061248:CDS | 20.0% |
| ! | AAATCAATCAGTTGATTAAG+GGG | + | chr8.3:6026847-6026866 | MS.gene061248:CDS | 25.0% |
| ! | CAAAATCAATCAGTTGATTA+AGG | + | chr8.3:6026845-6026864 | MS.gene061248:CDS | 25.0% |
| ! | TGAATGCAATTTGCATAATT+TGG | - | chr8.3:6026726-6026745 | None:intergenic | 25.0% |
| !!! | ATCAAATTAGCATTGGATTT+TGG | - | chr8.3:6026681-6026700 | None:intergenic | 25.0% |
| !!! | CAACTGATTGATTTTGTTTA+AGG | - | chr8.3:6026841-6026860 | None:intergenic | 25.0% |
| AATGCTAATGCTAATGCTAA+TGG | - | chr8.3:6026609-6026628 | None:intergenic | 30.0% | |
| TTGCTGTATCAAATTAGCAT+TGG | - | chr8.3:6026688-6026707 | None:intergenic | 30.0% | |
| ! | TGGTAGATACAAAGCAATTT+TGG | + | chr8.3:6026905-6026924 | MS.gene061248:CDS | 30.0% |
| !! | AATTAGCATTGGATTTTGGA+TGG | - | chr8.3:6026677-6026696 | None:intergenic | 30.0% |
| GATCCTAAATTTGCAGAAGA+TGG | + | chr8.3:6026969-6026988 | MS.gene061248:CDS | 35.0% | |
| ! | TCAAAGCTTCCTTTTGATCA+TGG | - | chr8.3:6026875-6026894 | None:intergenic | 35.0% |
| AACAGGTCTAGCACTAATCA+TGG | + | chr8.3:6026785-6026804 | MS.gene061248:CDS | 40.0% | |
| ACTTGTGAGAATGGCTTCAA+TGG | + | chr8.3:6027020-6027039 | MS.gene061248:CDS | 40.0% | |
| AGCTTCTTGGATCAGATTTG+AGG | - | chr8.3:6026752-6026771 | None:intergenic | 40.0% | |
| CTTGTGAGAATGGCTTCAAT+GGG | + | chr8.3:6027021-6027040 | MS.gene061248:CDS | 40.0% | |
| GCAATATTCTCATCAGAGAG+TGG | - | chr8.3:6027053-6027072 | None:intergenic | 40.0% | |
| GCACCATCTTCTGCAAATTT+AGG | - | chr8.3:6026975-6026994 | None:intergenic | 40.0% | |
| TGATGTAGCAGTCATAACAG+CGG | + | chr8.3:6027079-6027098 | MS.gene061248:CDS | 40.0% | |
| ! | GTTTAAGGCAGTGTTTGCTT+TGG | - | chr8.3:6026826-6026845 | None:intergenic | 40.0% |
| ! | TTGAGAGCTGCAACAGATTT+TGG | - | chr8.3:6026942-6026961 | None:intergenic | 40.0% |
| !! | GAAGCTTTGAATTCATGTGC+TGG | + | chr8.3:6026885-6026904 | MS.gene061248:CDS | 40.0% |
| ACATCTGCATCAGAGCTTCT+TGG | - | chr8.3:6026765-6026784 | None:intergenic | 45.0% | |
| AGGTCTAGCACTAATCATGG+TGG | + | chr8.3:6026788-6026807 | MS.gene061248:CDS | 45.0% | |
| GAAGCCACTACTTGTGAGAA+TGG | + | chr8.3:6027011-6027030 | MS.gene061248:CDS | 45.0% | |
| GAAGCCATTCTCACAAGTAG+TGG | - | chr8.3:6027018-6027037 | None:intergenic | 45.0% | |
| TAAGGGGAGCCATGATCAAA+AGG | + | chr8.3:6026863-6026882 | MS.gene061248:CDS | 45.0% | |
| ! | AGCTCTGATGCAGATGTAAC+AGG | + | chr8.3:6026768-6026787 | MS.gene061248:CDS | 45.0% |
| TCTGTTGCAGCTCTCAAGCA+AGG | + | chr8.3:6026945-6026964 | MS.gene061248:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 6026591 | 6027124 | 6026591 | ID=MS.gene061248 |
| chr8.3 | mRNA | 6026591 | 6027124 | 6026591 | ID=MS.gene061248.t1;Parent=MS.gene061248 |
| chr8.3 | exon | 6026591 | 6027124 | 6026591 | ID=MS.gene061248.t1.exon1;Parent=MS.gene061248.t1 |
| chr8.3 | CDS | 6026591 | 6027124 | 6026591 | ID=cds.MS.gene061248.t1;Parent=MS.gene061248.t1 |
| Gene Sequence |
| Protein sequence |
