AlfalfaGEDB | MS.gene49486

M. sativa cultivar XinJiangDaYe / MS.gene49486

KEGG: K20185
GO: GO:0005575 GO:0031082 GO:0031083 GO:0032991
PFAM: PF06320.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49486.t1	XP_003615993.2	93.4	106	7	0	1	106	1	106	8.00E-46	193

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49486.t1	O22929	55.2	96	43	0	11	106	26	121	4.0e-22	105.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49486.t1	G7KFM0	93.4	106	7	0	1	106	1	106	5.7e-46	193.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene49486	0.811728	6.06E-51	-1.69E-46
MS.gene049159	MS.gene49486	0.854145	1.50E-61	-1.69E-46
MS.gene04915	MS.gene49486	0.805512	1.29E-49	-1.69E-46
MS.gene04917	MS.gene49486	0.802048	6.75E-49	-1.69E-46
MS.gene049230	MS.gene49486	0.818909	1.53E-52	-1.69E-46
MS.gene049378	MS.gene49486	0.845105	5.00E-59	-1.69E-46
MS.gene049382	MS.gene49486	0.830995	2.14E-55	-1.69E-46
MS.gene049501	MS.gene49486	0.811815	5.80E-51	-1.69E-46
MS.gene049647	MS.gene49486	0.828479	8.79E-55	-1.69E-46
MS.gene049653	MS.gene49486	0.800663	1.30E-48	-1.69E-46
MS.gene049756	MS.gene49486	0.846407	2.21E-59	-1.69E-46
MS.gene04984	MS.gene49486	0.835087	2.06E-56	-1.69E-46
MS.gene049919	MS.gene49486	0.809171	2.16E-50	-1.69E-46
MS.gene050226	MS.gene49486	0.863816	1.90E-64	-1.69E-46
MS.gene050227	MS.gene49486	0.861175	1.23E-63	-1.69E-46
MS.gene050228	MS.gene49486	0.865093	7.58E-65	-1.69E-46
MS.gene050277	MS.gene49486	0.803616	3.20E-49	-1.69E-46
MS.gene050303	MS.gene49486	0.818377	2.02E-52	-1.69E-46
MS.gene050317	MS.gene49486	0.813539	2.43E-51	-1.69E-46
MS.gene050438	MS.gene49486	0.806957	6.39E-50	-1.69E-46
MS.gene050442	MS.gene49486	0.835225	1.90E-56	-1.69E-46
MS.gene050623	MS.gene49486	-0.842723	2.17E-58	-1.69E-46
MS.gene050629	MS.gene49486	-0.812464	4.19E-51	-1.69E-46
MS.gene050642	MS.gene49486	0.805944	1.05E-49	-1.69E-46
MS.gene050680	MS.gene49486	0.811576	6.53E-51	-1.69E-46
MS.gene050719	MS.gene49486	0.864126	1.52E-64	-1.69E-46
MS.gene050721	MS.gene49486	0.864217	1.43E-64	-1.69E-46
MS.gene050724	MS.gene49486	0.85871	6.84E-63	-1.69E-46
MS.gene050762	MS.gene49486	0.844382	7.83E-59	-1.69E-46
MS.gene050763	MS.gene49486	0.845378	4.22E-59	-1.69E-46
MS.gene050780	MS.gene49486	0.859897	3.01E-63	-1.69E-46
MS.gene050807	MS.gene49486	0.818707	1.70E-52	-1.69E-46
MS.gene050815	MS.gene49486	0.836347	9.87E-57	-1.69E-46
MS.gene050824	MS.gene49486	0.812844	3.45E-51	-1.69E-46
MS.gene050826	MS.gene49486	0.808767	2.63E-50	-1.69E-46
MS.gene050844	MS.gene49486	0.830161	3.43E-55	-1.69E-46
MS.gene050987	MS.gene49486	0.802859	4.59E-49	-1.69E-46
MS.gene051102	MS.gene49486	0.80124	9.89E-49	-1.69E-46
MS.gene051180	MS.gene49486	0.801222	9.97E-49	-1.69E-46
MS.gene051210	MS.gene49486	0.85536	6.65E-62	-1.69E-46
MS.gene051243	MS.gene49486	0.823234	1.55E-53	-1.69E-46
MS.gene051245	MS.gene49486	0.83984	1.25E-57	-1.69E-46
MS.gene051328	MS.gene49486	0.806046	9.95E-50	-1.69E-46
MS.gene051349	MS.gene49486	0.828735	7.62E-55	-1.69E-46
MS.gene051452	MS.gene49486	0.835221	1.90E-56	-1.69E-46
MS.gene051535	MS.gene49486	0.810578	1.08E-50	-1.69E-46
MS.gene051676	MS.gene49486	0.803481	3.42E-49	-1.69E-46
MS.gene051757	MS.gene49486	0.85694	2.29E-62	-1.69E-46
MS.gene051790	MS.gene49486	0.80788	4.07E-50	-1.69E-46
MS.gene051935	MS.gene49486	0.800337	1.51E-48	-1.69E-46
MS.gene052012	MS.gene49486	0.845839	3.16E-59	-1.69E-46
MS.gene052020	MS.gene49486	0.820212	7.73E-53	-1.69E-46
MS.gene052176	MS.gene49486	0.837741	4.35E-57	-1.69E-46
MS.gene052286	MS.gene49486	0.824351	8.46E-54	-1.69E-46
MS.gene052302	MS.gene49486	0.836285	1.02E-56	-1.69E-46
MS.gene052323	MS.gene49486	0.833167	6.23E-56	-1.69E-46
MS.gene052376	MS.gene49486	0.846824	1.70E-59	-1.69E-46
MS.gene052384	MS.gene49486	0.80996	1.46E-50	-1.69E-46
MS.gene052426	MS.gene49486	0.831783	1.37E-55	-1.69E-46
MS.gene052434	MS.gene49486	0.803006	4.28E-49	-1.69E-46
MS.gene052611	MS.gene49486	0.802696	4.96E-49	-1.69E-46
MS.gene052665	MS.gene49486	0.819868	9.26E-53	-1.69E-46
MS.gene052773	MS.gene49486	0.801662	8.10E-49	-1.69E-46
MS.gene052856	MS.gene49486	0.833437	5.34E-56	-1.69E-46
MS.gene052858	MS.gene49486	0.817181	3.77E-52	-1.69E-46
MS.gene052860	MS.gene49486	0.817241	3.65E-52	-1.69E-46
MS.gene052863	MS.gene49486	0.83784	4.10E-57	-1.69E-46
MS.gene052896	MS.gene49486	0.853244	2.72E-61	-1.69E-46
MS.gene053008	MS.gene49486	0.846809	1.72E-59	-1.69E-46
MS.gene053074	MS.gene49486	0.819344	1.22E-52	-1.69E-46
MS.gene053184	MS.gene49486	0.806554	7.78E-50	-1.69E-46
MS.gene053216	MS.gene49486	0.838598	2.61E-57	-1.69E-46
MS.gene053381	MS.gene49486	0.816298	5.94E-52	-1.69E-46
MS.gene05415	MS.gene49486	0.800706	1.27E-48	-1.69E-46
MS.gene054184	MS.gene49486	0.823082	1.68E-53	-1.69E-46
MS.gene054185	MS.gene49486	0.830471	2.88E-55	-1.69E-46
MS.gene054379	MS.gene49486	0.85527	7.06E-62	-1.69E-46
MS.gene054468	MS.gene49486	0.814238	1.71E-51	-1.69E-46
MS.gene054472	MS.gene49486	0.827186	1.80E-54	-1.69E-46
MS.gene054542	MS.gene49486	0.817795	2.74E-52	-1.69E-46
MS.gene05462	MS.gene49486	0.825963	3.52E-54	-1.69E-46
MS.gene054657	MS.gene49486	0.826471	2.66E-54	-1.69E-46
MS.gene054658	MS.gene49486	0.833736	4.49E-56	-1.69E-46
MS.gene05468	MS.gene49486	0.829923	3.92E-55	-1.69E-46
MS.gene054930	MS.gene49486	0.820477	6.72E-53	-1.69E-46
MS.gene05510	MS.gene49486	0.843152	1.67E-58	-1.69E-46
MS.gene05517	MS.gene49486	0.815864	7.43E-52	-1.69E-46
MS.gene055222	MS.gene49486	0.81213	4.95E-51	-1.69E-46
MS.gene055311	MS.gene49486	0.820957	5.21E-53	-1.69E-46
MS.gene055313	MS.gene49486	0.811317	7.44E-51	-1.69E-46
MS.gene055363	MS.gene49486	0.836441	9.34E-57	-1.69E-46
MS.gene055441	MS.gene49486	0.821608	3.69E-53	-1.69E-46
MS.gene055512	MS.gene49486	0.813513	2.46E-51	-1.69E-46
MS.gene055603	MS.gene49486	0.805274	1.45E-49	-1.69E-46
MS.gene055623	MS.gene49486	0.850916	1.25E-60	-1.69E-46
MS.gene055701	MS.gene49486	0.830187	3.38E-55	-1.69E-46
MS.gene055818	MS.gene49486	0.829919	3.93E-55	-1.69E-46
MS.gene055926	MS.gene49486	0.808586	2.88E-50	-1.69E-46
MS.gene05604	MS.gene49486	0.82797	1.17E-54	-1.69E-46
MS.gene056069	MS.gene49486	-0.823329	1.47E-53	-1.69E-46
MS.gene056400	MS.gene49486	0.807341	5.30E-50	-1.69E-46
MS.gene056432	MS.gene49486	0.801991	6.93E-49	-1.69E-46
MS.gene056506	MS.gene49486	0.838844	2.26E-57	-1.69E-46
MS.gene056552	MS.gene49486	0.845062	5.13E-59	-1.69E-46
MS.gene056589	MS.gene49486	0.806614	7.55E-50	-1.69E-46
MS.gene056631	MS.gene49486	0.802589	5.22E-49	-1.69E-46
MS.gene056760	MS.gene49486	0.862087	6.49E-64	-1.69E-46
MS.gene057026	MS.gene49486	0.818089	2.35E-52	-1.69E-46
MS.gene057055	MS.gene49486	0.812289	4.57E-51	-1.69E-46
MS.gene057057	MS.gene49486	0.832693	8.17E-56	-1.69E-46
MS.gene057060	MS.gene49486	0.805805	1.12E-49	-1.69E-46
MS.gene057140	MS.gene49486	0.846335	2.32E-59	-1.69E-46
MS.gene057234	MS.gene49486	0.802816	4.69E-49	-1.69E-46
MS.gene057360	MS.gene49486	0.830918	2.24E-55	-1.69E-46
MS.gene057394	MS.gene49486	0.814514	1.48E-51	-1.69E-46
MS.gene057395	MS.gene49486	0.800663	1.30E-48	-1.69E-46
MS.gene05761	MS.gene49486	0.805455	1.32E-49	-1.69E-46
MS.gene057693	MS.gene49486	0.829433	5.16E-55	-1.69E-46
MS.gene057750	MS.gene49486	0.808204	3.47E-50	-1.69E-46
MS.gene057754	MS.gene49486	0.824486	7.87E-54	-1.69E-46
MS.gene057869	MS.gene49486	0.8256	4.29E-54	-1.69E-46
MS.gene057915	MS.gene49486	0.81635	5.79E-52	-1.69E-46
MS.gene05795	MS.gene49486	0.802792	4.74E-49	-1.69E-46
MS.gene058055	MS.gene49486	0.800344	1.51E-48	-1.69E-46
MS.gene058084	MS.gene49486	0.82261	2.16E-53	-1.69E-46
MS.gene058146	MS.gene49486	0.832624	8.50E-56	-1.69E-46
MS.gene058168	MS.gene49486	0.827607	1.42E-54	-1.69E-46
MS.gene058182	MS.gene49486	0.846278	2.40E-59	-1.69E-46
MS.gene058238	MS.gene49486	0.815643	8.32E-52	-1.69E-46
MS.gene058399	MS.gene49486	0.848201	7.13E-60	-1.69E-46
MS.gene058595	MS.gene49486	0.826671	2.39E-54	-1.69E-46
MS.gene058636	MS.gene49486	0.808987	2.36E-50	-1.69E-46
MS.gene058906	MS.gene49486	0.860811	1.59E-63	-1.69E-46
MS.gene058979	MS.gene49486	0.808934	2.43E-50	-1.69E-46
MS.gene059124	MS.gene49486	0.818048	2.40E-52	-1.69E-46
MS.gene059128	MS.gene49486	0.818954	1.50E-52	-1.69E-46
MS.gene059161	MS.gene49486	0.802346	5.86E-49	-1.69E-46
MS.gene059249	MS.gene49486	0.831148	1.97E-55	-1.69E-46
MS.gene059317	MS.gene49486	0.810823	9.52E-51	-1.69E-46
MS.gene059335	MS.gene49486	0.801813	7.54E-49	-1.69E-46
MS.gene059713	MS.gene49486	0.854439	1.23E-61	-1.69E-46
MS.gene059725	MS.gene49486	0.828385	9.26E-55	-1.69E-46
MS.gene059789	MS.gene49486	0.838993	2.07E-57	-1.69E-46
MS.gene059830	MS.gene49486	0.845888	3.07E-59	-1.69E-46
MS.gene059842	MS.gene49486	0.802195	6.29E-49	-1.69E-46
MS.gene059898	MS.gene49486	0.808171	3.53E-50	-1.69E-46
MS.gene060084	MS.gene49486	0.825752	3.95E-54	-1.69E-46
MS.gene060296	MS.gene49486	0.804326	2.28E-49	-1.69E-46
MS.gene060297	MS.gene49486	0.817325	3.50E-52	-1.69E-46
MS.gene060310	MS.gene49486	0.813011	3.18E-51	-1.69E-46
MS.gene060322	MS.gene49486	0.847923	8.50E-60	-1.69E-46
MS.gene06052	MS.gene49486	0.814654	1.38E-51	-1.69E-46
MS.gene060572	MS.gene49486	0.827964	1.17E-54	-1.69E-46
MS.gene060586	MS.gene49486	0.822	2.99E-53	-1.69E-46
MS.gene060619	MS.gene49486	0.813116	3.01E-51	-1.69E-46
MS.gene060637	MS.gene49486	0.847192	1.35E-59	-1.69E-46
MS.gene060683	MS.gene49486	0.860721	1.69E-63	-1.69E-46
MS.gene060685	MS.gene49486	0.855214	7.33E-62	-1.69E-46
MS.gene060781	MS.gene49486	0.801196	1.01E-48	-1.69E-46
MS.gene060802	MS.gene49486	0.824541	7.63E-54	-1.69E-46
MS.gene060803	MS.gene49486	0.850036	2.20E-60	-1.69E-46
MS.gene060869	MS.gene49486	0.817521	3.16E-52	-1.69E-46
MS.gene060870	MS.gene49486	0.824241	8.98E-54	-1.69E-46
MS.gene060907	MS.gene49486	0.813155	2.95E-51	-1.69E-46
MS.gene060942	MS.gene49486	0.817964	2.51E-52	-1.69E-46
MS.gene060998	MS.gene49486	0.876584	1.24E-68	-1.69E-46
MS.gene061248	MS.gene49486	0.804637	1.96E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49486.t1	MTR_5g074880	90.826	109	10	0	1	109	1	109	2.60e-68	201

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49486.t1	AT2G30330	55.294	85	38	0	22	106	37	121	5.37e-28	100
MS.gene49486.t1	AT2G30330	55.294	85	38	0	22	106	37	121	8.26e-28	99.8

Find 15 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGATGATGAATGTGAATT+AGG	0.250869	5.1:+57744280	None:intergenic
CGCGTCTACAACTGGACGTT+TGG	0.267025	5.1:-57743591	MS.gene49486:CDS
TAGCCCTTCGCGTCTACAAC+TGG	0.355789	5.1:-57743599	MS.gene49486:CDS
AAGAAAAGCAGAGCGCGTTT+CGG	0.423070	5.1:-57744208	MS.gene49486:CDS
GCGTCTACAACTGGACGTTT+GGG	0.446986	5.1:-57743590	MS.gene49486:CDS
TTCGAGATTTGTGAAACAAA+CGG	0.473259	5.1:-57744091	MS.gene49486:CDS
TTCTCTTAACACTGCTCTCA+AGG	0.509830	5.1:-57744046	MS.gene49486:intron
ACACGCAGAATCAAAGGGAT+TGG	0.536514	5.1:-57744319	MS.gene49486:CDS
TAACACACACGCAGAATCAA+AGG	0.552685	5.1:-57744325	MS.gene49486:CDS
CTGTTAGTAGAAGCAGTGAA+TGG	0.564549	5.1:-57744183	MS.gene49486:CDS
AACACACACGCAGAATCAAA+GGG	0.575682	5.1:-57744324	MS.gene49486:CDS
TTGTGAAACAAACGGATCAG+TGG	0.597090	5.1:-57744083	MS.gene49486:CDS
ACGTCCAGTTGTAGACGCGA+AGG	0.599148	5.1:+57743595	None:intergenic
CGTCCAGTTGTAGACGCGAA+GGG	0.609212	5.1:+57743596	None:intergenic
TTAGTAGAAGCAGTGAATGG+CGG	0.707209	5.1:-57744180	MS.gene49486:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTAGTTAATAATGATA+AGG	-	chr5.1:57744111-57744130	MS.gene49486:CDS	10.0%
!!	ATTGATAAGTTAGCTTATAG+CGG	-	chr5.1:57743994-57744013	MS.gene49486:intron	25.0%
!!!	TTTGATGATGAATGTGAATT+AGG	+	chr5.1:57743648-57743667	None:intergenic	25.0%
	CTGATTGAATTTGTAGTGTT+TGG	-	chr5.1:57744056-57744075	MS.gene49486:CDS	30.0%
	TTCGAGATTTGTGAAACAAA+CGG	-	chr5.1:57743834-57743853	MS.gene49486:intron	30.0%
	AGCTCATTTGAAAGTCTGTT+TGG	+	chr5.1:57744266-57744285	None:intergenic	35.0%
!!	TATAGCGGATGAACTTATAG+AGG	-	chr5.1:57744009-57744028	MS.gene49486:intron	35.0%
!!!	TGCATCTTTCTTCGCTTTTT+CGG	+	chr5.1:57743695-57743714	None:intergenic	35.0%
	AACACACACGCAGAATCAAA+GGG	-	chr5.1:57743601-57743620	MS.gene49486:intron	40.0%
	ACTTCATCACGAGCTTATAG+CGG	+	chr5.1:57744168-57744187	None:intergenic	40.0%
	CTGTTAGTAGAAGCAGTGAA+TGG	-	chr5.1:57743742-57743761	MS.gene49486:intron	40.0%
	TAACACACACGCAGAATCAA+AGG	-	chr5.1:57743600-57743619	MS.gene49486:intron	40.0%
	TTAGTAGAAGCAGTGAATGG+CGG	-	chr5.1:57743745-57743764	MS.gene49486:intron	40.0%
	TTCTCTTAACACTGCTCTCA+AGG	-	chr5.1:57743879-57743898	MS.gene49486:intron	40.0%
	TTGTGAAACAAACGGATCAG+TGG	-	chr5.1:57743842-57743861	MS.gene49486:intron	40.0%
!!!	CTTCGCTTTTTCGGTTTGAT+TGG	+	chr5.1:57743686-57743705	None:intergenic	40.0%
	AAGAAAAGCAGAGCGCGTTT+CGG	-	chr5.1:57743717-57743736	MS.gene49486:intron	45.0%
	ACACGCAGAATCAAAGGGAT+TGG	-	chr5.1:57743606-57743625	MS.gene49486:intron	45.0%
	GCGTCTACAACTGGACGTTT+GGG	-	chr5.1:57744335-57744354	MS.gene49486:CDS	50.0%
	ACGTCCAGTTGTAGACGCGA+AGG	+	chr5.1:57744333-57744352	None:intergenic	55.0%
	CGCGTCTACAACTGGACGTT+TGG	-	chr5.1:57744334-57744353	MS.gene49486:CDS	55.0%
	CGTCCAGTTGTAGACGCGAA+GGG	+	chr5.1:57744332-57744351	None:intergenic	55.0%
	TAGCCCTTCGCGTCTACAAC+TGG	-	chr5.1:57744326-57744345	MS.gene49486:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	57743583	57744364	57743583	ID=MS.gene49486
chr5.1	mRNA	57743583	57744364	57743583	ID=MS.gene49486.t1;Parent=MS.gene49486
chr5.1	exon	57744047	57744364	57744047	ID=MS.gene49486.t1.exon1;Parent=MS.gene49486.t1
chr5.1	CDS	57744047	57744364	57744047	ID=cds.MS.gene49486.t1;Parent=MS.gene49486.t1
chr5.1	exon	57743583	57743618	57743583	ID=MS.gene49486.t1.exon2;Parent=MS.gene49486.t1
chr5.1	CDS	57743583	57743618	57743583	ID=cds.MS.gene49486.t1;Parent=MS.gene49486.t1

Gene Sequence

>MS.gene49486

ATGGAAGAACGCGATGTTAACACACACGCAGAATCAAAGGGATTGGAAGATTCATTACAAAACCTAATTCACATTCATCATCAAAAATCTCTCATTCTCACCAATCAAACCGAAAAAGCGAAGAAAGATGCAATAAGAAAAGCAGAGCGCGTTTCGGATCTGTTAGTAGAAGCAGTGAATGGCGGTGTTCAAGATTCGTTTATTTATCAGAAGAGAATTGAGCTTGAAATTCGTGCTCTTGCTGTAACTATTTCGAGATTTGTGAAACAAACGGATCAGTGGCTTAATGCTACTCATTCTCTTAACACTGCTCTCAAGGTAACAAATTGCTTTCATTTTTTGTGTATTCTTTAATTTTGTGTTTTCTTCATTATTGTGTTCAAATCTCTGCTTTGTGAAAAATAGCGAATAATTGATAAGTTAGCTTATAGCGGATGAACTTATAGAGGAGAGTTGATTAGCAAATAAGCTAGCTGATTGAATTTGTAGTGTTTGGTAAAATTAACTGTTCAACTTTCTAAAGATTCAATTTTTAGTTAATAATGATAAGGTACAAGCTGCTTGAATTAGCTTTATCAAAAACCGCTATAAGCTCGTGATGAAGTGACGATTACAAAACATGTCTGTTTTAGTTTAATGAGCTTATTAGACATAAGCTATAAGCTCAAAAACATGTCTTACCAAACAGACTTTCAAATGAGCTACTTGTTAAGTGACAAATGACTTTCAAGTTTCAACATACATAGCCCTTCGCGTCTACAACTGGACGTTTGGGAGCGTGA

Protein sequence

>MS.gene49486.t1

MEERDVNTHAESKGLEDSLQNLIHIHHQKSLILTNQTEKAKKDAIRKAERVSDLLVEAVNGGVQDSFIYQKRIELEIRALAVTISRFVKQTDQWLNATHSLNTALKPFASTTGRLGA