AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061272

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061272.t1 MTR_4g122070 90.737 1112 93 3 1 1105 1 1109 0.0 2020
MS.gene061272.t1 MTR_2g450210 31.925 213 92 6 144 307 306 514 1.02e-21 102
MS.gene061272.t1 MTR_2g450210 31.925 213 92 6 144 307 306 514 1.34e-21 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061272.t1 AT3G66652 29.572 257 94 5 55 306 82 256 6.10e-22 103
MS.gene061272.t1 AT3G66652 29.572 257 94 5 55 306 82 256 6.10e-22 103
MS.gene061272.t1 AT3G66652 29.572 257 94 5 55 306 82 256 6.10e-22 103
MS.gene061272.t1 AT5G58040 25.967 362 169 13 55 329 184 533 8.09e-16 83.6
MS.gene061272.t1 AT5G58040 25.967 362 169 13 55 329 184 533 9.05e-16 83.2

Find 241 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAAGCAATTTCTTATTT+TGG 0.136303 8.3:-6513153 None:intergenic
AATCCGGTGTCTTGATTAAT+AGG 0.151869 8.3:+6510937 MS.gene061272:CDS
TGAACCGCAGATTGGTAATT+TGG 0.166594 8.3:+6513339 MS.gene061272:CDS
TTCAAGGCATTCCTCCTTTC+CGG 0.178616 8.3:+6510125 MS.gene061272:CDS
ATTCTGATTATGCGCATATT+TGG 0.187022 8.3:-6510439 None:intergenic
TCTTGACACATTAGCTAAAA+TGG 0.190473 8.3:+6513375 MS.gene061272:CDS
GATGAACGACTGCAATATTT+TGG 0.199819 8.3:+6509793 MS.gene061272:CDS
ACAAAACAGTCTTGCTATTA+TGG 0.205720 8.3:+6509853 MS.gene061272:CDS
CTTCAGAACCTTTCAGATAA+AGG 0.241730 8.3:+6513169 MS.gene061272:CDS
GGGAATATGAAGACAGATTC+AGG 0.251532 8.3:+6509932 MS.gene061272:CDS
TAGAATAATGTATCATCTTC+AGG 0.255082 8.3:-6510726 None:intergenic
TTCTGATTATGCGCATATTT+GGG 0.258920 8.3:-6510438 None:intergenic
GAATAATAAGATGTATTTAC+TGG 0.268929 8.3:-6509601 None:intergenic
GAGTTCTGACACTGGCTTAA+AGG 0.272628 8.3:+6509525 MS.gene061272:CDS
GAAGAGAGTGAATTAATTTC+AGG 0.287879 8.3:+6508036 MS.gene061272:CDS
CAATTTGATCAACCTGTATC+AGG 0.289591 8.3:+6507563 MS.gene061272:CDS
CCTGATCAATGCGCTCTTCC+TGG 0.303559 8.3:-6506744 None:intergenic
CCTCCTTTCCGGCTAAAGAT+AGG 0.305122 8.3:+6510136 MS.gene061272:CDS
CTCCAGAACTTTACCATCTT+CGG 0.306536 8.3:-6509308 None:intergenic
CCAAACAATATTATCATTGT+TGG 0.326368 8.3:-6510685 None:intergenic
AAGAAGAGAATCTCACAAAA+TGG 0.328097 8.3:+6513307 MS.gene061272:CDS
TGGTTATTGTCCAAACGTTC+AGG 0.333850 8.3:-6506724 None:intergenic
ATAACAGATTACTTCAATTT+CGG 0.334106 8.3:+6507403 MS.gene061272:CDS
TGATCACCAAGTGATAATTC+TGG 0.335553 8.3:-6509397 None:intergenic
CTTTACCATCTTCGGAATAT+CGG 0.338370 8.3:-6509300 None:intergenic
TCAAGTTCCAGTTACTTTGC+TGG 0.342297 8.3:+6507180 MS.gene061272:CDS
TTCCGAAGATGGTAAAGTTC+TGG 0.353899 8.3:+6509306 MS.gene061272:CDS
GAAGCAATACTGTTCGTCTT+TGG 0.355159 8.3:+6507444 MS.gene061272:CDS
AGATTCCTTCCACTGGATCT+AGG 0.358130 8.3:-6509733 None:intergenic
GGAAAGATGTTCCATATGAT+TGG 0.360190 8.3:+6509827 MS.gene061272:CDS
AAAAGATCCAGCAAAGTAAC+TGG 0.367382 8.3:-6507187 None:intergenic
GTGGAAGGAATCTGTCTTCC+CGG 0.374128 8.3:+6509743 MS.gene061272:CDS
AGATTCTGATGTTATTATAC+AGG 0.376619 8.3:+6507866 MS.gene061272:CDS
AAACAATCCGAAGTGCCTTC+TGG 0.376840 8.3:+6508129 MS.gene061272:CDS
TTCAGAACCTTTCAGATAAA+GGG 0.378762 8.3:+6513170 MS.gene061272:CDS
CCAACAATGATAATATTGTT+TGG 0.381761 8.3:+6510685 MS.gene061272:CDS
GAATATCGGTCCTCTTCATC+TGG 0.383681 8.3:-6509286 None:intergenic
TGTTGTTCATCCTGAACGTT+TGG 0.386120 8.3:+6506714 MS.gene061272:CDS
AATGTTTGTGAACAATATTA+TGG 0.387283 8.3:+6507080 MS.gene061272:CDS
GAGAAGCCGTGGAGGATTCC+CGG 0.388121 8.3:+6507373 MS.gene061272:CDS
CCTGAGTCTCCTCTCATATA+GGG 0.390031 8.3:+6510086 MS.gene061272:CDS
CCACGGGGTATCCACATCTA+TGG 0.390852 8.3:-6510391 None:intergenic
GATGTGGATACCCCGTGGTC+TGG 0.401754 8.3:+6510396 MS.gene061272:CDS
CTTGCATGTCTTTCGTGCAT+TGG 0.403446 8.3:-6510759 None:intergenic
ACCTGAGTCTCCTCTCATAT+AGG 0.415425 8.3:+6510085 MS.gene061272:CDS
TGTTGTTGCGGAGGAGGAGG+TGG 0.417036 8.3:-6506565 None:intergenic
ATCCGAAGTGCCTTCTGGTC+AGG 0.417510 8.3:+6508134 MS.gene061272:CDS
TAGCCCCTTAAGAAGTCATT+TGG 0.418795 8.3:+6509501 MS.gene061272:CDS
TGCAGAGAAGCGTAAAGCTT+TGG 0.420136 8.3:+6513066 MS.gene061272:CDS
TATACAGTGAATATTCAGAT+AGG 0.422164 8.3:+6509911 MS.gene061272:CDS
ATTATGGCTAATAAGTCAAT+GGG 0.422170 8.3:+6507096 MS.gene061272:CDS
ACCTGTATTGCTCTTCCCAT+TGG 0.424082 8.3:-6507786 None:intergenic
ACTCACCAAATGACTTCTTA+AGG 0.424522 8.3:-6509506 None:intergenic
CTTTCTGCCCTTTATCTGAA+AGG 0.424602 8.3:-6513177 None:intergenic
TGCCTCTGATCCTGTTGTAC+TGG 0.428982 8.3:-6509637 None:intergenic
TATTATGGCTAATAAGTCAA+TGG 0.430254 8.3:+6507095 MS.gene061272:CDS
AAATAGAGGTCCAGTACAAC+AGG 0.432030 8.3:+6509627 MS.gene061272:CDS
CCAGGAAGAGCGCATTGATC+AGG 0.432191 8.3:+6506744 MS.gene061272:CDS
TAAAGGGCAGAAAGAAGGTT+TGG 0.432450 8.3:+6513186 MS.gene061272:CDS
CTCACCAAATGACTTCTTAA+GGG 0.438527 8.3:-6509505 None:intergenic
ACTGACTCGGCAGTTGATAT+AGG 0.441153 8.3:+6513502 MS.gene061272:CDS
AACTGTGTCAGAGGTGGTTA+CGG 0.441261 8.3:+6506817 MS.gene061272:CDS
TGGGAAGAGCAATACAGGTT+GGG 0.441844 8.3:+6507790 MS.gene061272:CDS
CTCAGAATCATTGTCAGAAT+AGG 0.443847 8.3:-6509437 None:intergenic
GCCAATGGGAAGAGCAATAC+AGG 0.444425 8.3:+6507785 MS.gene061272:intron
CCAGTCATTGCCTCTGTTGG+TGG 0.451413 8.3:-6510562 None:intergenic
TGTAAACATTGATAAATTTG+AGG 0.452481 8.3:+6507351 MS.gene061272:CDS
TGTATCATTTCGCATCAGTC+TGG 0.454029 8.3:-6513020 None:intergenic
GCATGTCTTTCGTGCATTGG+AGG 0.455104 8.3:-6510756 None:intergenic
GAAAGTAAGCTCCCTGATAC+AGG 0.455319 8.3:-6507575 None:intergenic
TGTTGCGGAGGAGGAGGTGG+AGG 0.455574 8.3:-6506562 None:intergenic
GAAACTGTGATTCTGATCAA+TGG 0.455890 8.3:+6510205 MS.gene061272:CDS
GAGGAGTGGCTAGAACTTGT+AGG 0.456687 8.3:-6506543 None:intergenic
GAAAGATGTTCCATATGATT+GGG 0.457654 8.3:+6509828 MS.gene061272:CDS
GATGACGGCAGTGGCGGCGG+TGG 0.463184 8.3:+6506598 MS.gene061272:CDS
CTCCTTTCCGGCTAAAGATA+GGG 0.464638 8.3:+6510137 MS.gene061272:CDS
GAAATAGATCCTTGCATGCT+AGG 0.464757 8.3:+6510781 MS.gene061272:CDS
ATGTGGATACCCCGTGGTCT+GGG 0.466318 8.3:+6510397 MS.gene061272:CDS
GACGATACCGAGAGCGAAGA+TGG 0.468920 8.3:+6506640 MS.gene061272:CDS
GTCCAGTGTGAAACCTCATA+TGG 0.473357 8.3:-6510663 None:intergenic
ATCCTCAGAAACAACTCTTC+TGG 0.473395 8.3:-6513263 None:intergenic
AATATAATGAAGATGACGAT+TGG 0.473462 8.3:+6510028 MS.gene061272:CDS
CCTCCGTTCTCCCAGACCAC+GGG 0.480663 8.3:-6510407 None:intergenic
ATATAATGAAGATGACGATT+GGG 0.482597 8.3:+6510029 MS.gene061272:CDS
TCAGATAAAGGGCAGAAAGA+AGG 0.486125 8.3:+6513181 MS.gene061272:CDS
GACAGGAGCAGAAGTGTTGT+CGG 0.489261 8.3:-6507989 None:intergenic
TAAAACTCGCACTCATCTAC+AGG 0.489960 8.3:-6510633 None:intergenic
CTGATTCTGCAGTCAGCAAA+AGG 0.494129 8.3:+6512995 MS.gene061272:intron
TGAAATAAAGCAACAGAGGC+CGG 0.494386 8.3:+6513525 MS.gene061272:CDS
CACTGTATACTGCCTGATCC+AGG 0.495185 8.3:-6509897 None:intergenic
CATTGCCTCTGTTGGTGGCA+TGG 0.496012 8.3:-6510557 None:intergenic
ATCCGGTGTCTTGATTAATA+GGG 0.496870 8.3:+6510938 MS.gene061272:CDS
TCAAGTACGTTACATTTGAC+AGG 0.497382 8.3:-6508006 None:intergenic
AAATGTTGATGACCTGGATC+AGG 0.498206 8.3:+6509885 MS.gene061272:CDS
TACTTCGTTATTCGAACCGA+TGG 0.500813 8.3:-6513458 None:intergenic
TCTTATTATTCAAGAAATAG+AGG 0.501104 8.3:+6509613 MS.gene061272:CDS
TCATGGTGTTGTTGCGGAGG+AGG 0.501315 8.3:-6506571 None:intergenic
GCTTCTTTATTCATACCGAT+TGG 0.501483 8.3:-6513424 None:intergenic
TTCCAGCGTCTCTTTCGAAC+CGG 0.503289 8.3:-6513544 None:intergenic
TGGTGTTGTTGCGGAGGAGG+AGG 0.504083 8.3:-6506568 None:intergenic
AATAATTCTGAACCGCAGAT+TGG 0.504224 8.3:+6513331 MS.gene061272:CDS
TGAGCTTCCCTATCTTTAGC+CGG 0.505864 8.3:-6510144 None:intergenic
TTCATCTTTCGCTCAAGGAA+GGG 0.513627 8.3:+6507134 MS.gene061272:CDS
TACCCCGTGGTCTGGGAGAA+CGG 0.515959 8.3:+6510404 MS.gene061272:CDS
GATTCCTTCCACTGGATCTA+GGG 0.516557 8.3:-6509732 None:intergenic
CTATTCTGACAATGATTCTG+AGG 0.517781 8.3:+6509438 MS.gene061272:CDS
CAGGATCAGAGGCATCAAAC+TGG 0.518562 8.3:+6509646 MS.gene061272:CDS
AGTCAAGGCAGTTTCATTCA+AGG 0.518925 8.3:+6510109 MS.gene061272:CDS
TCCTCCGTTCTCCCAGACCA+CGG 0.520629 8.3:-6510408 None:intergenic
TATTGCATCTCAGCACCGTC+GGG 0.520792 8.3:-6510914 None:intergenic
CCCTATATGAGAGGAGACTC+AGG 0.520802 8.3:-6510086 None:intergenic
CTGCCAGTCATTGCCTCTGT+TGG 0.521166 8.3:-6510565 None:intergenic
GTGCTGAGATGCAATAAATC+CGG 0.521449 8.3:+6510921 MS.gene061272:CDS
TCATCTTTCGCTCAAGGAAG+GGG 0.521933 8.3:+6507135 MS.gene061272:CDS
TCACAATCGCAGAAATGACC+TGG 0.522617 8.3:+6509237 MS.gene061272:intron
AGATAGAACATCATGTTCAC+TGG 0.522652 8.3:-6508085 None:intergenic
GTTCATCTTTCGCTCAAGGA+AGG 0.523958 8.3:+6507133 MS.gene061272:CDS
AGAGCACAAGAACGATATAC+AGG 0.524508 8.3:+6510342 MS.gene061272:CDS
TTGATTATTCTTCAGCCAAT+GGG 0.526661 8.3:+6507771 MS.gene061272:intron
CCACCAACAGAGGCAATGAC+TGG 0.526705 8.3:+6510562 MS.gene061272:CDS
GATTGTTCATCTTTCGCTCA+AGG 0.528423 8.3:+6507129 MS.gene061272:CDS
GTAATCTGTTATGTCGGCAC+CGG 0.528884 8.3:-6507392 None:intergenic
TTGTCCTGCTGCTTGTGTTG+AGG 0.530125 8.3:+6506690 MS.gene061272:CDS
TTCCCTATCTTTAGCCGGAA+AGG 0.530551 8.3:-6510139 None:intergenic
GAAGGTTTGGATGTAGAAGA+AGG 0.531483 8.3:+6513199 MS.gene061272:CDS
TTGTAGTTTATGAAGACTCA+AGG 0.533121 8.3:+6507054 MS.gene061272:intron
GACAATGATGACGGCAGTGG+CGG 0.534907 8.3:+6506592 MS.gene061272:CDS
CTTGATTAATAGGGAAGGAA+AGG 0.535406 8.3:+6510947 MS.gene061272:CDS
TTATTGCATCTCAGCACCGT+CGG 0.535767 8.3:-6510915 None:intergenic
AAATAGATCCTTGCATGCTA+GGG 0.536684 8.3:+6510782 MS.gene061272:CDS
CACTGCCGTCATCATTGTCA+TGG 0.537216 8.3:-6506588 None:intergenic
AGAATAATGTATCATCTTCA+GGG 0.538295 8.3:-6510725 None:intergenic
AATTCTGGTTCACTACAGCC+TGG 0.538311 8.3:-6509382 None:intergenic
GTCCAGTACAACAGGATCAG+AGG 0.538398 8.3:+6509635 MS.gene061272:CDS
TCTTTGCACCCTAGCATGCA+AGG 0.538465 8.3:-6510790 None:intergenic
AATGATGACGGCAGTGGCGG+CGG 0.538576 8.3:+6506595 MS.gene061272:CDS
TAGCTAAAATGGAGAAACGT+AGG 0.542189 8.3:+6513386 MS.gene061272:CDS
AATTCAGAATCCAGATGAAG+AGG 0.542516 8.3:+6509276 MS.gene061272:CDS
GAAGGGGTAGACATCATGAC+AGG 0.543323 8.3:+6510364 MS.gene061272:CDS
GGGAAGAGCAATACAGGTTG+GGG 0.545158 8.3:+6507791 MS.gene061272:CDS
GGAATATGAAGACAGATTCA+GGG 0.549642 8.3:+6509933 MS.gene061272:CDS
GTTCGAAAGAGACGCTGGAA+TGG 0.549713 8.3:+6513547 MS.gene061272:CDS
GACACATGCAGTGAAGATCC+AGG 0.551473 8.3:+6509364 MS.gene061272:CDS
TTCCCTATTAATCAAGACAC+CGG 0.551477 8.3:-6510940 None:intergenic
TCGGTTTAAATGAGAACACT+TGG 0.552338 8.3:+6507422 MS.gene061272:CDS
GAGAAGCGTAAAGCTTTGGT+AGG 0.552922 8.3:+6513070 MS.gene061272:CDS
ATTCAAACCATCTTCGCTCT+CGG 0.554356 8.3:-6506647 None:intergenic
ATGGGAAGAGCAATACAGGT+TGG 0.556587 8.3:+6507789 MS.gene061272:CDS
GGAAGACAGATTCCTTCCAC+TGG 0.557364 8.3:-6509740 None:intergenic
TTCCTGACCAGAAGGCACTT+CGG 0.558677 8.3:-6508136 None:intergenic
ATTGAAGTAATCTGTTATGT+CGG 0.559276 8.3:-6507398 None:intergenic
TTTGGGCAGATCAATGTGCA+TGG 0.559726 8.3:+6507961 MS.gene061272:intron
AGCTAAAATGGAGAAACGTA+GGG 0.561211 8.3:+6513387 MS.gene061272:CDS
TAATCTGTTATGTCGGCACC+GGG 0.562693 8.3:-6507391 None:intergenic
GCGTGGACGCCTCACATCTA+CGG 0.563983 8.3:-6507838 None:intergenic
AAAGGCAAATAATGCATCAC+AGG 0.564082 8.3:+6510166 MS.gene061272:CDS
CACTTCAAAATGTGGATCGA+GGG 0.564304 8.3:+6513041 MS.gene061272:CDS
AAGTGCCTTCTGGTCAGGAA+AGG 0.564474 8.3:+6508139 MS.gene061272:CDS
TTGGGCAGATCAATGTGCAT+GGG 0.564891 8.3:+6507962 MS.gene061272:intron
TCCTCTCATATAGGGAGTCA+AGG 0.565123 8.3:+6510094 MS.gene061272:CDS
AAATGATACACTTCAAAATG+TGG 0.566953 8.3:+6513033 MS.gene061272:CDS
CGGAGGAGGAGGTGGAGGAG+TGG 0.567757 8.3:-6506557 None:intergenic
CAAGAACGATATACAGGAAG+GGG 0.569062 8.3:+6510348 MS.gene061272:CDS
ACTGATGCTGCTGAAGATGA+AGG 0.569899 8.3:+6510597 MS.gene061272:CDS
ATACAGTGAATATTCAGATA+GGG 0.571628 8.3:+6509912 MS.gene061272:CDS
GAGGACCGATATTCCGAAGA+TGG 0.571654 8.3:+6509295 MS.gene061272:CDS
TGTGAAACCTCATATGGGAG+GGG 0.574793 8.3:-6510657 None:intergenic
TCATTCCTCAAAATGTGAAG+TGG 0.575525 8.3:+6507598 MS.gene061272:CDS
TCACCAAATGACTTCTTAAG+GGG 0.576985 8.3:-6509504 None:intergenic
CATCTTTCGCTCAAGGAAGG+GGG 0.578969 8.3:+6507136 MS.gene061272:CDS
GGCCGGTTCGAAAGAGACGC+TGG 0.578971 8.3:+6513542 MS.gene061272:CDS
ACATCCTCAACACAAGCAGC+AGG 0.579788 8.3:-6506694 None:intergenic
AGATGATACATTATTCTATG+AGG 0.580067 8.3:+6510731 MS.gene061272:CDS
CATTTGGTGAGTTCTGACAC+TGG 0.580187 8.3:+6509517 MS.gene061272:CDS
TCTGATTATGCGCATATTTG+GGG 0.580429 8.3:-6510437 None:intergenic
TCCAGTGTGAAACCTCATAT+GGG 0.582401 8.3:-6510662 None:intergenic
CACAAGAACGATATACAGGA+AGG 0.586945 8.3:+6510346 MS.gene061272:CDS
TGTAGTTTATGAAGACTCAA+GGG 0.588636 8.3:+6507055 MS.gene061272:intron
TGTTCTATCTGATGAACAGT+TGG 0.590396 8.3:+6508098 MS.gene061272:CDS
TATTCTGACAATGATTCTGA+GGG 0.593340 8.3:+6509439 MS.gene061272:CDS
GTGTGAAACCTCATATGGGA+GGG 0.594584 8.3:-6510658 None:intergenic
CATGACAATGATGACGGCAG+TGG 0.594877 8.3:+6506589 MS.gene061272:CDS
AATTAACAAATCGATGACAC+CGG 0.595250 8.3:-6509762 None:intergenic
TTCTCGCTCAGCAAAAGAAG+AGG 0.597493 8.3:-6510271 None:intergenic
GCCTCACATCTACGGATGGT+TGG 0.597566 8.3:-6507830 None:intergenic
GTGAAACCTCATATGGGAGG+GGG 0.601096 8.3:-6510656 None:intergenic
AATTTGATCAACCTGTATCA+GGG 0.603259 8.3:+6507564 MS.gene061272:CDS
ACAAACCTTTCCTGACCAGA+AGG 0.610940 8.3:-6508144 None:intergenic
TGACAGGGCCTCCATAGATG+TGG 0.613366 8.3:+6510380 MS.gene061272:CDS
TTGTCATGGTGTTGTTGCGG+AGG 0.614553 8.3:-6506574 None:intergenic
TCATTGTCATGGTGTTGTTG+CGG 0.614911 8.3:-6506577 None:intergenic
CGAAGATGGTAAAGTTCTGG+AGG 0.617888 8.3:+6509309 MS.gene061272:CDS
TGACAGAAATGTTGATGACC+TGG 0.620403 8.3:+6509879 MS.gene061272:CDS
ATCTTTCGCTCAAGGAAGGG+GGG 0.621812 8.3:+6507137 MS.gene061272:CDS
CCTATCTTTAGCCGGAAAGG+AGG 0.622134 8.3:-6510136 None:intergenic
GATACAGAAACTTCGACGGA+TGG 0.622411 8.3:-6513241 None:intergenic
CTAAAGATAGGGAAGCTCAA+AGG 0.628869 8.3:+6510148 MS.gene061272:CDS
TTTGAAGTTGAACACTGACT+CGG 0.629197 8.3:+6513489 MS.gene061272:CDS
TCGGCACCGGGAATCCTCCA+CGG 0.630361 8.3:-6507379 None:intergenic
ACACTTCAAAATGTGGATCG+AGG 0.630743 8.3:+6513040 MS.gene061272:CDS
GGTGTCTTGATTAATAGGGA+AGG 0.633166 8.3:+6510942 MS.gene061272:CDS
TCTCACATCCCTAGATCCAG+TGG 0.634148 8.3:+6509724 MS.gene061272:CDS
ATAAATTTGAGGAGAAGCCG+TGG 0.635122 8.3:+6507362 MS.gene061272:CDS
ACATCCCTAGATCCAGTGGA+AGG 0.638284 8.3:+6509728 MS.gene061272:CDS
GGACGCCTCACATCTACGGA+TGG 0.638825 8.3:-6507834 None:intergenic
ACTTCGTTATTCGAACCGAT+GGG 0.638949 8.3:-6513457 None:intergenic
CAAACTGGTAGACATATGCC+CGG 0.638995 8.3:+6509661 MS.gene061272:CDS
ACAAGAACGATATACAGGAA+GGG 0.640237 8.3:+6510347 MS.gene061272:CDS
TCTGGATACAGAAACTTCGA+CGG 0.640452 8.3:-6513245 None:intergenic
GCCAACCATCCGTAGATGTG+AGG 0.640599 8.3:+6507829 MS.gene061272:CDS
GCCTTGACTCCCTATATGAG+AGG 0.642152 8.3:-6510095 None:intergenic
ATCCAGAAGAGTTGTTTCTG+AGG 0.643385 8.3:+6513261 MS.gene061272:CDS
TCTCGCTCAGCAAAAGAAGA+GGG 0.645386 8.3:-6510270 None:intergenic
CAACACCATGACAATGATGA+CGG 0.646110 8.3:+6506583 MS.gene061272:CDS
AGTGTGAAACCTCATATGGG+AGG 0.646826 8.3:-6510659 None:intergenic
ATTAACAAATCGATGACACC+GGG 0.647422 8.3:-6509761 None:intergenic
CTCCGTTCTCCCAGACCACG+GGG 0.647651 8.3:-6510406 None:intergenic
GAAGCAGTGAAGCAGCCCAT+CGG 0.655244 8.3:+6513442 MS.gene061272:CDS
AGTGGCTAGAACTTGTAGGT+CGG 0.656034 8.3:-6506539 None:intergenic
CTGTCCAAATTACCAATCTG+CGG 0.656360 8.3:-6513343 None:intergenic
TGAACGTTTGGACAATAACC+AGG 0.658846 8.3:+6506726 MS.gene061272:CDS
AGTGAACCAGAATTATCACT+TGG 0.659426 8.3:+6509391 MS.gene061272:CDS
TCCCATATGAGGTTTCACAC+TGG 0.665363 8.3:+6510661 MS.gene061272:CDS
CGGGGTATCCACATCTATGG+AGG 0.665605 8.3:-6510388 None:intergenic
CATAACCATGCCACCAACAG+AGG 0.665694 8.3:+6510552 MS.gene061272:CDS
AAATGACCTGGATCCTGATG+TGG 0.667202 8.3:+6509249 MS.gene061272:CDS
CCCGTGGTCTGGGAGAACGG+AGG 0.668646 8.3:+6510407 MS.gene061272:CDS
TAGAGATCACTCTCACCCGA+CGG 0.671020 8.3:+6510899 MS.gene061272:CDS
TTGATCAAATTGCTCCTGTG+TGG 0.673387 8.3:-6507552 None:intergenic
GTAAACACTGACCAATGCCA+AGG 0.674544 8.3:-6507213 None:intergenic
TAGGTGAAATAAAGCAACAG+AGG 0.677763 8.3:+6513521 MS.gene061272:CDS
AATACAGGTTGGGGACAGTG+TGG 0.682082 8.3:+6507800 MS.gene061272:CDS
AGTTCTGATGCACAACATTG+TGG 0.683317 8.3:+6506781 MS.gene061272:CDS
ACATCAGAATCTATACTGCG+TGG 0.694629 8.3:-6507855 None:intergenic
AGAGGCAATGACTGGCAGTG+TGG 0.695843 8.3:+6510570 MS.gene061272:CDS
AATTTGAGGAGAAGCCGTGG+AGG 0.697052 8.3:+6507365 MS.gene061272:CDS
CCATAGATGTGGATACCCCG+TGG 0.697300 8.3:+6510391 MS.gene061272:CDS
AAGGGGTAGACATCATGACA+GGG 0.710725 8.3:+6510365 MS.gene061272:CDS
AGGAGCAGAAGTGTTGTCGG+AGG 0.712271 8.3:-6507986 None:intergenic
TTATGGCTAATAAGTCAATG+GGG 0.760772 8.3:+6507097 MS.gene061272:CDS
ATATGAAGACAGATTCAGGG+AGG 0.765745 8.3:+6509936 MS.gene061272:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.3 gene 6506484 6513568 6506484 ID=MS.gene061272
chr8.3 mRNA 6506484 6513568 6506484 ID=MS.gene061272.t1;Parent=MS.gene061272
chr8.3 exon 6506484 6506861 6506484 ID=MS.gene061272.t1.exon1;Parent=MS.gene061272.t1
chr8.3 CDS 6506484 6506861 6506484 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6507060 6507223 6507060 ID=MS.gene061272.t1.exon2;Parent=MS.gene061272.t1
chr8.3 CDS 6507060 6507223 6507060 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6507339 6507465 6507339 ID=MS.gene061272.t1.exon3;Parent=MS.gene061272.t1
chr8.3 CDS 6507339 6507465 6507339 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6507548 6507619 6507548 ID=MS.gene061272.t1.exon4;Parent=MS.gene061272.t1
chr8.3 CDS 6507548 6507619 6507548 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6507786 6507887 6507786 ID=MS.gene061272.t1.exon5;Parent=MS.gene061272.t1
chr8.3 CDS 6507786 6507887 6507786 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6507970 6508160 6507970 ID=MS.gene061272.t1.exon6;Parent=MS.gene061272.t1
chr8.3 CDS 6507970 6508160 6507970 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6509249 6510968 6509249 ID=MS.gene061272.t1.exon7;Parent=MS.gene061272.t1
chr8.3 CDS 6509249 6510968 6509249 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
chr8.3 exon 6513007 6513568 6513007 ID=MS.gene061272.t1.exon8;Parent=MS.gene061272.t1
chr8.3 CDS 6513007 6513568 6513007 ID=cds.MS.gene061272.t1;Parent=MS.gene061272.t1
Gene Sequence

>MS.gene061272

ATGGAACAAGATGATTTCACCCACGATGATGATGATGATTTTGGAGAACTTTACGCCGACCTACAAGTTCTAGCCACTCCTCCACCTCCTCCTCCGCAACAACACCATGACAATGATGACGGCAGTGGCGGCGGTGGCGATTCAAACAACAACGACGACGATACCGAGAGCGAAGATGGTTTGAATATTGTTTTAAACGACGATGATTGTCCTGCTGCTTGTGTTGAGGATGTTGTTCATCCTGAACGTTTGGACAATAACCAGGAAGAGCGCATTGATCAGGAACAACAATTTGTGAGTTCTGATGCACAACATTGTGGTCAGAGTAGTAAAAACTGTGTCAGAGGTGGTTACGGTTCACAATTTTTTCGCTCTAAGGTTCGTATTCTGCTTCTTTTTCTATTTCAACTTTATGCTTTTAATTACTGGAATGGATCCTCTCGTGTGTAATTTTCAAATAGCATTTCTGTCTCAATAATTGATATTACAAGCGACATTGTGGAAGCATTTATGCTGTTGATTGAAACATGTGTTTAATGATTAATTTGTTTATTATGATTATGATTTATTTTGTAGTTTATGAAGACTCAAGGGTCAATGTTTGTGAACAATATTATGGCTAATAAGTCAATGGGGTTGACTAAAGATTGTTCATCTTTCGCTCAAGGAAGGGGGGATAGTTTTCAGAATCATAAATCAAGTTCCAGTTACTTTGCTGGATCTTTTCTTCCTTGGCATTGGTCAGTGTTTACATTCTCTCATGCTGTTCTTCATGCATATTTTTTTTATGTGTTTTATTCATTATTGGATTCAATTATGTTATCTTCTTAGCTGACTTCTTCCCCTTCCTTACAGGAAGATATATGATGTAAACATTGATAAATTTGAGGAGAAGCCGTGGAGGATTCCCGGTGCCGACATAACAGATTACTTCAATTTCGGTTTAAATGAGAACACTTGGAAGCAATACTGTTCGTCTTTGGTGAGTTCATTACTTCATTTTGTAGCAATGTGTAAAACCAGTCGAGTTGTTTGACCTTGCTTACTGGATTTGGTATTTTCAGGCATCCACACAGGAGCAATTTGATCAACCTGTATCAGGGAGCTTACTTTCTCATTCCTCAAAATGTGAAGTGGTATGCTTATGCTGATAGTAATTGCCACATTTTAATCATTTCAATTTTGATTTCTGTCAAAAAAAATCATTTCAATTTTGATAGTTTTATAATTCACTTGACACTTTCTTCTTAACTGTTGAATGCAAATTTGACATTGGGACTTTTGTGATTGATTATTCTTCAGCCAATGGGAAGAGCAATACAGGTTGGGGACAGTGTGGTTGATCGCCAACCATCCGTAGATGTGAGGCGTCCACGCAGTATAGATTCTGATGTTATTATACAGGTTTGCACTCACGCTAAGTTTGCCATGACTCTTGTGCTGTAGTTGTCATACGGTGATAGTAATCATGGGTGTCTTTGGGCAGATCAATGTGCATGGGTCCTCCGACAACACTTCTGCTCCTGTCAAATGTAACGTACTTGACTCATCTGAAGAGAGTGAATTAATTTCAGGAGTTAACATAAACAAAAGCAATTCTTCCAGTGAACATGATGTTCTATCTGATGAACAGTTGGAAGATGCTAAACAATCCGAAGTGCCTTCTGGTCAGGAAAGGTTTGTTATTTGTCTGATGATTTCAGTGATACCTTATATATGGTGTATGTGATGTATTATGACAACTCCAGTTGTAGGATGTAGACCTGCTTTCACTTAGTTAGAGCTTTTGTGTGTTTCTCCCTTGTTAGAAGTATGACCAACGGCACCAAAATAACAATGAAAACTAACATCATTAGGTCAAAGATAAGAACCCCTAAAGAGATACATAATGATTAAGAGAAAATAGCCCCTTTACAAAAATGTTCCTCACCCTCAACCTTCAATTTCCTTCCTCAGCTCCAGATCACTGAAGCGGTCTGAAGCTAATCTTCCACTGTGTCAAAAGCTCCATCATTAGGTTTCCTTAACCCGTTTTACCTCATTAGGATTAAAAAAATATTTTTTTAATAAATGAAATAGAAGAGCCACAAACCAAGTTGCTTGGGCTCCAGTGGCAAGGAGCTCCTTCCAAGGACGTACCTGGGGAATACCGGGTTCGATCCCCAGGGGGAACAACGCTTGGCCAGTGTGCATGCCTCTACGCACGAGCCGGATTAGTCGCCCACCATTGGTAGGTTGGAAACCGGTGCAAAAGCCAAAAAAAAACAAAAAAATAGAAGAGCCACAACTGTATCTAAATGAGACACGGTGGTTTCATGGCCATTTCAGAGTTCTTGCACTCGTGACACTAACACAGCTGTGTATCGTTTGTGAGCTTTACGCACAGCTGTGTCAAGATGGAGCATAGTTGTGTCTTTTGGAGATTTGAACTCAGACATCATTTTTTATTCTGCATTGGAAAATATCATATTTGATGGATATGAAATGATTTTTACTAGTCAAACTTGAGACATGAAATCAGACACTATTTTAATTTACTGATACTGATTAGTACCTTCCATTCTATGGACAATCACATCTTTTATATAGTCGCAAGCATTTCTTGTTACAAACCGATTCTTCATTGTAATTCGTTAAAGACTTCTAGGATTTTCTCCTTTTTAAATATAAATTCCTGAATAGAATTTACCGTGGAAATTGTTTGTTTGTTAGGGAGTTAAACATATAACGTAACTGATGTAGACTTTGATTTTTCACAATCGCAGAAATGACCTGGATCCTGATGTGGTTAAAATTCAGAATCCAGATGAAGAGGACCGATATTCCGAAGATGGTAAAGTTCTGGAGGAAGAAATAAAAACAGAGGGTAGAGTTTGTATTGACACATGCAGTGAAGATCCAGGCTGTAGTGAACCAGAATTATCACTTGGTGATCAAGAACTTAGTCTGACTTCCTATTCTGACAATGATTCTGAGGGAACTGAAGACAGTTTACATGTTTATAATGAGAGAAAGCATAGCCCCTTAAGAAGTCATTTGGTGAGTTCTGACACTGGCTTAAAGGAGTCTTTGCCTCTTTATGAGAAAACCTCAAGAAAAATCAGTGTCAACAGAAAACCAGTAAATACATCTTATTATTCAAGAAATAGAGGTCCAGTACAACAGGATCAGAGGCATCAAACTGGTAGACATATGCCCGGTTCTAAGCTGCAAAAACACATGGAAAATGATAATAATGTTTCTCACATCCCTAGATCCAGTGGAAGGAATCTGTCTTCCCGGTGTCATCGATTTGTTAATTACAGATCAGATGAACGACTGCAATATTTTGGAAGTCATGAAAGGAAAGATGTTCCATATGATTGGGAAACAAAACAGTCTTGCTATTATGGTACTGACAGAAATGTTGATGACCTGGATCAGGCAGTATACAGTGAATATTCAGATAGGGAATATGAAGACAGATTCAGGGAGGATAAAAATCAATATATCAGAAAAAGTGGGGATAAGAGGGAGTATTTTTTTGAGCGAAGAACTCTAATAAAATATAATGAAGATGACGATTGGGATCCTGCTAGTAGAAAACAGTATGTTGATGATGACCTGAGTCTCCTCTCATATAGGGAGTCAAGGCAGTTTCATTCAAGGCATTCCTCCTTTCCGGCTAAAGATAGGGAAGCTCAAAGGCAAATAATGCATCACAGGCGTCATTTTAAGGACAGAAACTGTGATTCTGATCAATGGTTTGATGATGAGTTTGAATTTTTAAACAAAAGTTATCGATATCCCTCTTCTTTTGCTGAGCGAGAAATGGAATCTTTGAATAATAGACATGAAGAGCAGTTTCTACAAATTGATAGAGCACAAGAACGATATACAGGAAGGGGTAGACATCATGACAGGGCCTCCATAGATGTGGATACCCCGTGGTCTGGGAGAACGGAGGATGAGTTCCCCAAATATGCGCATAATCAGAATTCATATTTGAAGTATCAGAGACAATCTTATACTGATTCAGCGAGAAATTATATGCACAGAGAAGATGATAGTTTTAGGGGACATGAGACACATAACCATGCCACCAACAGAGGCAATGACTGGCAGTGTGGTTATACTGATGCTGCTGAAGATGAAGGTTGCACAACTTCTCCTGTAGATGAGTGCGAGTTTTACCCCCTCCCATATGAGGTTTCACACTGGACCAACAATGATAATATTGTTTGGAATCATGATGACATGCACCCTGAAGATGATACATTATTCTATGAGGAACCTCCAATGCACGAAAGACATGCAAGAAATAGATCCTTGCATGCTAGGGTGCAAAGAAATGATATAAAACTACAGCAGCATCAGCTAAATTTTTCAAGGAAAGGTGGGGACATCTTTATAAAAAGAAGCTCTAAGGTTATGAGTAGAGATCACTCTCACCCGACGGTGCTGAGATGCAATAAATCCGGTGTCTTGATTAATAGGGAAGGAAAGGTAAAATTAGGGAAATTATGGTTAAAAACCTATACTGCTGTACTTTTTTATCCATATGAGCTACTCAACATCTCTTGGATTCCTCATTCAATATATATTGTACCACTGCCCGCATGCAGCTATATATTGTTCTTATCTTTACCTTCTCTATTTTTATTCTTTTAAAATAGTTAAGATTAAATGACCTTAAGTTCAGAATCTTATCCAAATTGAGGAGGATTTAAAGTTAAAAACTGAAAGCAAGTCCATGAAGTCATTATAGCATAAGTCTCGGACTTCCTTTCCAAAATAAAACATGATATTTCATGGCTTGAAGATGTCACGTATAGTCCTATTTATCATATTGTTTGGTATTAAATTCATGATTTAATATTTTTCCTTTTGTTATAGCTGATTTTTCTGCTTTTACTCAACTTAGTTTTATTCGGGGTGTACCTTGAAAAAGAGCCTTAGCGCAACAGTTGGAATTGCTGTCATGTGACTAAGAGGTTTACAGGTTTGTGGAATCAGCCTCTTGCTCATGCAAAGTAAGGCTACCTTCACTAGATCTGTAATGGGTCTTTGGTGGGATTTATGCCGCCTAAAAATTAATATATGGGCTGTGCTGGGAATCTGACATATTGTAGAATGGGTTGTGGTGATCATGATTGCTATCACCAACATGGATAGTTAGCATGGCTGATCAGGGTCTAGTTACATCCTTTGATCTTTTGTCTTGATATAGTTATGTGGCTTATGGTATGTTTTTTCTATGTAATTTTTATTTTTATATTTTAATTGATTACAGCTTTTCCACTGGACATTTTATTTTGGTTTCACATCTTTGATTGATTTTCTGAACTTCTTAACCAGTCTATACCTTGCAACAAGAGTCCACTACATAATCATGTATACAGTAAAGTCCCAAAAAATATTAAGAATAACCTTGGGTGTGTGTGTTTGTGTGACATCAAGCATGTGGTGTAGCCAAAAAAACATATGTCACTGTTTTATGTCCTTCAAATATGAACTCACATTTTCTTCCTTACTTTTCAAGTTTATATTTAAATTTATTTTATGTTTGAATGCATGGATCTGATAGGTTGTCATGTTTTATGTCCTTCAAATATCTGCACTGGTGTTCAAGTAGAAGTGTTATTTTTGTGTGTGTAGAGAGTGGACTCTTACATGTTTGGTCCAATTCATGGATCTTGTATGGTACTAAATAAGTTTCTGGCAATGAGGACAATATCTTCTTAGCCATTTGTGCTCATCTGGAAATGTTGTCTCTCCGTATGGCATTTTAAAGATATTTACACTGTGATGACTAGGTTCTAGCTATGCATTGTGTCTATTTCATGTATTTTAGTTTACTGCTAAAAATGGTTTAATGAATAATTGGTAGTTTGGGTATGTATTATTGTTAAGATTTGTTCTGTAAAGAACTTGATCATTATTTACTCAAATCAATATGTTGGAGTCCCATTTACATGATTGGAATATATTGGTCTGATATAACTGCTTCAGTGTCATTTATACATTTAGTTCTTAAACTTCTCATGATTAGATTGATTTAACAGCATTTACCTCTAAAAATGAATGACACGCATAATATATTTTCATCTTCTGTTGAGTATATGAGCCAGCATAGGTATAAGTTGCGTTGTTATTATGTTTACCCTAATTTTTATGATCAGAAATCTACTATTCTATTTTGTAGTGGGTAACTTGTAGATGTTTTCCTTGAAATAGTTTTACCTAACATGTTGTTTCTGTTTCTGTCACACTTTAATGACTTCCTGTTTCTGTCAAGCATTGTATTAGTATCTATATTTATTCATATCAGGCATTCTTACTCAGTAACTCTTTATCATACCTCATTGTTTTATTCATATCAGGGCATTCTAACTCGGTAACTCTCTTTATCATACCTCATTGTTGCACTTTCAATCATTATTTGATGCATTATAGTGCTAGTTCTAGAACAGTGGTTTTTATTTGTTAGATAATTGTGTTTTGATAATAAATTTATGAGGTACCCTTGTGACTGATTCTGCAGTCAGCAAAAGGTTCCAGACTGATGCGAAATGATACACTTCAAAATGTGGATCGAGGGATTGCAGAGAAGCGTAAAGCTTTGGTAGGTTTTGATGACTCTCGTAAGAAGGCCATCAAACTTGACGTCTCAAAATCTCAGCGTGTCGACCAAAATAAGAAATTGCTTCAGAACCTTTCAGATAAAGGGCAGAAAGAAGGTTTGGATGTAGAAGAAGGTGAGATAGTAACAGAAGAACCATCCGTCGAAGTTTCTGTATCCAGAAGAGTTGTTTCTGAGGATGCAACACTTGCTGAAAGCGTGAAGAAGAGAATCTCACAAAATGGAAATAATTCTGAACCGCAGATTGGTAATTTGGACAGCCAGAAAATTCTTGACACATTAGCTAAAATGGAGAAACGTAGGGAGCGTTTTAAGCAGCCAATCGGTATGAATAAAGAAGCAGTGAAGCAGCCCATCGGTTCGAATAACGAAGTAGTGAAGAGTTTGAAGTTGAACACTGACTCGGCAGTTGATATAGGTGAAATAAAGCAACAGAGGCCGGTTCGAAAGAGACGCTGGAATG
Protein sequence

>MS.gene061272.t1

MEQDDFTHDDDDDFGELYADLQVLATPPPPPPQQHHDNDDGSGGGGDSNNNDDDTESEDGLNIVLNDDDCPAACVEDVVHPERLDNNQEERIDQEQQFVSSDAQHCGQSSKNCVRGGYGSQFFRSKFMKTQGSMFVNNIMANKSMGLTKDCSSFAQGRGDSFQNHKSSSSYFAGSFLPWHWKIYDVNIDKFEEKPWRIPGADITDYFNFGLNENTWKQYCSSLASTQEQFDQPVSGSLLSHSSKCEVPMGRAIQVGDSVVDRQPSVDVRRPRSIDSDVIIQINVHGSSDNTSAPVKCNVLDSSEESELISGVNINKSNSSSEHDVLSDEQLEDAKQSEVPSGQERNDLDPDVVKIQNPDEEDRYSEDGKVLEEEIKTEGRVCIDTCSEDPGCSEPELSLGDQELSLTSYSDNDSEGTEDSLHVYNERKHSPLRSHLVSSDTGLKESLPLYEKTSRKISVNRKPVNTSYYSRNRGPVQQDQRHQTGRHMPGSKLQKHMENDNNVSHIPRSSGRNLSSRCHRFVNYRSDERLQYFGSHERKDVPYDWETKQSCYYGTDRNVDDLDQAVYSEYSDREYEDRFREDKNQYIRKSGDKREYFFERRTLIKYNEDDDWDPASRKQYVDDDLSLLSYRESRQFHSRHSSFPAKDREAQRQIMHHRRHFKDRNCDSDQWFDDEFEFLNKSYRYPSSFAEREMESLNNRHEEQFLQIDRAQERYTGRGRHHDRASIDVDTPWSGRTEDEFPKYAHNQNSYLKYQRQSYTDSARNYMHREDDSFRGHETHNHATNRGNDWQCGYTDAAEDEGCTTSPVDECEFYPLPYEVSHWTNNDNIVWNHDDMHPEDDTLFYEEPPMHERHARNRSLHARVQRNDIKLQQHQLNFSRKGGDIFIKRSSKVMSRDHSHPTVLRCNKSGVLINREGKSAKGSRLMRNDTLQNVDRGIAEKRKALVGFDDSRKKAIKLDVSKSQRVDQNKKLLQNLSDKGQKEGLDVEEGEIVTEEPSVEVSVSRRVVSEDATLAESVKKRISQNGNNSEPQIGNLDSQKILDTLAKMEKRRERFKQPIGMNKEAVKQPIGSNNEVVKSLKLNTDSAVDIGEIKQQRPVRKRRWN