Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061328.t1 | XP_013455686.1 | 100 | 71 | 0 | 0 | 1 | 71 | 119 | 189 | 1.20E-28 | 135.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061328.t1 | A0A072UJ52 | 100.0 | 71 | 0 | 0 | 1 | 71 | 119 | 189 | 8.6e-29 | 135.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061100 | MS.gene061328 | 0.800389 | 1.47E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061328.t1 | MTR_4g050050 | 100.000 | 71 | 0 | 0 | 1 | 71 | 86 | 156 | 1.39e-44 | 140 |
| MS.gene061328.t1 | MTR_4g050050 | 100.000 | 71 | 0 | 0 | 1 | 71 | 119 | 189 | 1.47e-44 | 141 |
| MS.gene061328.t1 | MTR_2g024090 | 75.862 | 58 | 14 | 0 | 1 | 58 | 110 | 167 | 2.58e-25 | 92.0 |
| MS.gene061328.t1 | MTR_2g450100 | 60.345 | 58 | 23 | 0 | 1 | 58 | 118 | 175 | 1.58e-17 | 72.0 |
| MS.gene061328.t1 | MTR_7g113900 | 52.632 | 57 | 27 | 0 | 3 | 59 | 106 | 162 | 7.18e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061328.t1 | AT1G05070 | 76.812 | 69 | 16 | 0 | 1 | 69 | 110 | 178 | 1.01e-31 | 108 |
| MS.gene061328.t1 | AT2G32580 | 76.119 | 67 | 16 | 0 | 3 | 69 | 49 | 115 | 2.80e-31 | 105 |
| MS.gene061328.t1 | AT2G32580 | 76.119 | 67 | 16 | 0 | 3 | 69 | 111 | 177 | 3.12e-30 | 105 |
| MS.gene061328.t1 | AT4G04360 | 68.750 | 64 | 20 | 0 | 3 | 66 | 112 | 175 | 4.13e-25 | 91.7 |
| MS.gene061328.t1 | AT4G30996 | 63.793 | 58 | 21 | 0 | 1 | 58 | 113 | 170 | 1.37e-20 | 80.1 |
| MS.gene061328.t1 | AT2G24290 | 60.345 | 58 | 23 | 0 | 1 | 58 | 114 | 171 | 8.40e-19 | 75.5 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTCTTTCCATCCTTTC+TGG | 0.011737 | 4.2:+51709176 | None:intergenic |
| GAAGCAGACAAGTGTAATTC+TGG | 0.342626 | 4.2:-51709289 | MS.gene061328:CDS |
| TCCATCCTTTCTGGCGAGCT+CGG | 0.384489 | 4.2:+51709185 | None:intergenic |
| GAAGAAGGTAACTTCTTTGT+GGG | 0.465794 | 4.2:-51709212 | MS.gene061328:CDS |
| CGCCAGAAAGGATGGAAAGA+AGG | 0.480798 | 4.2:-51709178 | MS.gene061328:CDS |
| GGCTCTGCTTGAAGCAAAGA+AGG | 0.487452 | 4.2:-51709332 | MS.gene061328:CDS |
| TCCGAGCTCGCCAGAAAGGA+TGG | 0.499503 | 4.2:-51709186 | MS.gene061328:CDS |
| TGGAAACGTGTGAGCAGGCA+AGG | 0.502793 | 4.2:-51709264 | MS.gene061328:CDS |
| AGAAGAAGGTAACTTCTTTG+TGG | 0.508555 | 4.2:-51709213 | MS.gene061328:CDS |
| GGCAAGGGAGAAAGCCGAGT+TGG | 0.512433 | 4.2:-51709248 | MS.gene061328:CDS |
| TGGTATGGAAACGTGTGAGC+AGG | 0.518723 | 4.2:-51709269 | MS.gene061328:CDS |
| GCCAGAAAGGATGGAAAGAA+GGG | 0.533520 | 4.2:-51709177 | MS.gene061328:CDS |
| GGTCGCATCTCAGTATCAAA+AGG | 0.534079 | 4.2:-51709311 | MS.gene061328:CDS |
| GGCATTAGTAGCTCAGAAGA+AGG | 0.541518 | 4.2:-51709227 | MS.gene061328:CDS |
| CTGAGCTACTAATGCCAACT+CGG | 0.549126 | 4.2:+51709234 | None:intergenic |
| AGACAAGTGTAATTCTGGTA+TGG | 0.560534 | 4.2:-51709284 | MS.gene061328:CDS |
| GAACTCCGAGCTCGCCAGAA+AGG | 0.561229 | 4.2:-51709190 | MS.gene061328:CDS |
| AAATCTAATACTCAGTCTCA+AGG | 0.601505 | 4.2:-51709148 | MS.gene061328:CDS |
| GGAAACGTGTGAGCAGGCAA+GGG | 0.646238 | 4.2:-51709263 | MS.gene061328:CDS |
| CCAGAAAGGATGGAAAGAAG+GGG | 0.659202 | 4.2:-51709176 | MS.gene061328:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCTAATACTCAGTCTCA+AGG | - | chr4.2:51709330-51709349 | MS.gene061328:CDS | 30.0% | |
| AGAAGAAGGTAACTTCTTTG+TGG | - | chr4.2:51709265-51709284 | MS.gene061328:CDS | 35.0% | |
| AGACAAGTGTAATTCTGGTA+TGG | - | chr4.2:51709194-51709213 | MS.gene061328:CDS | 35.0% | |
| GAAGAAGGTAACTTCTTTGT+GGG | - | chr4.2:51709266-51709285 | MS.gene061328:CDS | 35.0% | |
| GAAGCAGACAAGTGTAATTC+TGG | - | chr4.2:51709189-51709208 | MS.gene061328:CDS | 40.0% | |
| CCAGAAAGGATGGAAAGAAG+GGG | - | chr4.2:51709302-51709321 | MS.gene061328:CDS | 45.0% | |
| CTGAGCTACTAATGCCAACT+CGG | + | chr4.2:51709247-51709266 | None:intergenic | 45.0% | |
| GCCAGAAAGGATGGAAAGAA+GGG | - | chr4.2:51709301-51709320 | MS.gene061328:CDS | 45.0% | |
| GGCATTAGTAGCTCAGAAGA+AGG | - | chr4.2:51709251-51709270 | MS.gene061328:CDS | 45.0% | |
| GGTCGCATCTCAGTATCAAA+AGG | - | chr4.2:51709167-51709186 | MS.gene061328:CDS | 45.0% | |
| CCCCTTCTTTCCATCCTTTC+TGG | + | chr4.2:51709305-51709324 | None:intergenic | 50.0% | |
| CGCCAGAAAGGATGGAAAGA+AGG | - | chr4.2:51709300-51709319 | MS.gene061328:CDS | 50.0% | |
| GGCTCTGCTTGAAGCAAAGA+AGG | - | chr4.2:51709146-51709165 | MS.gene061328:CDS | 50.0% | |
| TGGTATGGAAACGTGTGAGC+AGG | - | chr4.2:51709209-51709228 | MS.gene061328:CDS | 50.0% | |
| GGAAACGTGTGAGCAGGCAA+GGG | - | chr4.2:51709215-51709234 | MS.gene061328:CDS | 55.0% | |
| TGGAAACGTGTGAGCAGGCA+AGG | - | chr4.2:51709214-51709233 | MS.gene061328:CDS | 55.0% | |
| ! | TCCATCCTTTCTGGCGAGCT+CGG | + | chr4.2:51709296-51709315 | None:intergenic | 55.0% |
| GAACTCCGAGCTCGCCAGAA+AGG | - | chr4.2:51709288-51709307 | MS.gene061328:CDS | 60.0% | |
| GGCAAGGGAGAAAGCCGAGT+TGG | - | chr4.2:51709230-51709249 | MS.gene061328:CDS | 60.0% | |
| TCCGAGCTCGCCAGAAAGGA+TGG | - | chr4.2:51709292-51709311 | MS.gene061328:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 51709144 | 51709356 | 51709144 | ID=MS.gene061328 |
| chr4.2 | mRNA | 51709144 | 51709356 | 51709144 | ID=MS.gene061328.t1;Parent=MS.gene061328 |
| chr4.2 | exon | 51709144 | 51709356 | 51709144 | ID=MS.gene061328.t1.exon1;Parent=MS.gene061328.t1 |
| chr4.2 | CDS | 51709144 | 51709356 | 51709144 | ID=cds.MS.gene061328.t1;Parent=MS.gene061328.t1 |
| Gene Sequence |
| Protein sequence |
