Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061329.t1 | XP_013455686.1 | 100 | 71 | 0 | 0 | 1 | 71 | 119 | 189 | 1.20E-28 | 135.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061329.t1 | A0A072UJ52 | 100.0 | 71 | 0 | 0 | 1 | 71 | 119 | 189 | 8.6e-29 | 135.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene061100 | MS.gene061329 | 0.804967 | 1.68E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061329.t1 | MTR_4g050050 | 100.000 | 71 | 0 | 0 | 1 | 71 | 86 | 156 | 1.39e-44 | 140 |
| MS.gene061329.t1 | MTR_4g050050 | 100.000 | 71 | 0 | 0 | 1 | 71 | 119 | 189 | 1.47e-44 | 141 |
| MS.gene061329.t1 | MTR_2g024090 | 75.862 | 58 | 14 | 0 | 1 | 58 | 110 | 167 | 2.58e-25 | 92.0 |
| MS.gene061329.t1 | MTR_2g450100 | 60.345 | 58 | 23 | 0 | 1 | 58 | 118 | 175 | 1.58e-17 | 72.0 |
| MS.gene061329.t1 | MTR_7g113900 | 52.632 | 57 | 27 | 0 | 3 | 59 | 106 | 162 | 7.18e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061329.t1 | AT1G05070 | 76.812 | 69 | 16 | 0 | 1 | 69 | 110 | 178 | 1.01e-31 | 108 |
| MS.gene061329.t1 | AT2G32580 | 76.119 | 67 | 16 | 0 | 3 | 69 | 49 | 115 | 2.80e-31 | 105 |
| MS.gene061329.t1 | AT2G32580 | 76.119 | 67 | 16 | 0 | 3 | 69 | 111 | 177 | 3.12e-30 | 105 |
| MS.gene061329.t1 | AT4G04360 | 68.750 | 64 | 20 | 0 | 3 | 66 | 112 | 175 | 4.13e-25 | 91.7 |
| MS.gene061329.t1 | AT4G30996 | 63.793 | 58 | 21 | 0 | 1 | 58 | 113 | 170 | 1.37e-20 | 80.1 |
| MS.gene061329.t1 | AT2G24290 | 60.345 | 58 | 23 | 0 | 1 | 58 | 114 | 171 | 8.40e-19 | 75.5 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTCTTTCCATCCTTTC+TGG | 0.011737 | 4.2:+51692279 | None:intergenic |
| GAAGCAGACAAGTGTAATTC+TGG | 0.342626 | 4.2:-51692392 | MS.gene061329:CDS |
| TCCATCCTTTCTGGCGAGCT+CGG | 0.384489 | 4.2:+51692288 | None:intergenic |
| GAAGAAGGTAACTTCTTTGT+GGG | 0.465794 | 4.2:-51692315 | MS.gene061329:CDS |
| CGCCAGAAAGGATGGAAAGA+AGG | 0.480798 | 4.2:-51692281 | MS.gene061329:CDS |
| GGCTCTGCTTGAAGCAAAGA+AGG | 0.487452 | 4.2:-51692435 | MS.gene061329:CDS |
| TCCGAGCTCGCCAGAAAGGA+TGG | 0.499503 | 4.2:-51692289 | MS.gene061329:CDS |
| TGGAAACGTGTGAGCAGGCA+AGG | 0.502793 | 4.2:-51692367 | MS.gene061329:CDS |
| AGAAGAAGGTAACTTCTTTG+TGG | 0.508555 | 4.2:-51692316 | MS.gene061329:CDS |
| GGCAAGGGAGAAAGCCGAGT+TGG | 0.512433 | 4.2:-51692351 | MS.gene061329:CDS |
| TGGTATGGAAACGTGTGAGC+AGG | 0.518723 | 4.2:-51692372 | MS.gene061329:CDS |
| GCCAGAAAGGATGGAAAGAA+GGG | 0.533520 | 4.2:-51692280 | MS.gene061329:CDS |
| GGTCGCATCTCAGTATCAAA+AGG | 0.534079 | 4.2:-51692414 | MS.gene061329:CDS |
| GGCATTAGTAGCTCAGAAGA+AGG | 0.541518 | 4.2:-51692330 | MS.gene061329:CDS |
| CTGAGCTACTAATGCCAACT+CGG | 0.549126 | 4.2:+51692337 | None:intergenic |
| AGACAAGTGTAATTCTGGTA+TGG | 0.560534 | 4.2:-51692387 | MS.gene061329:CDS |
| GAACTCCGAGCTCGCCAGAA+AGG | 0.561229 | 4.2:-51692293 | MS.gene061329:CDS |
| AAATCTAATACTCAGTCTCA+AGG | 0.601505 | 4.2:-51692251 | MS.gene061329:CDS |
| GGAAACGTGTGAGCAGGCAA+GGG | 0.646238 | 4.2:-51692366 | MS.gene061329:CDS |
| CCAGAAAGGATGGAAAGAAG+GGG | 0.659202 | 4.2:-51692279 | MS.gene061329:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCTAATACTCAGTCTCA+AGG | - | chr4.2:51692433-51692452 | MS.gene061329:CDS | 30.0% | |
| AGAAGAAGGTAACTTCTTTG+TGG | - | chr4.2:51692368-51692387 | MS.gene061329:CDS | 35.0% | |
| AGACAAGTGTAATTCTGGTA+TGG | - | chr4.2:51692297-51692316 | MS.gene061329:CDS | 35.0% | |
| GAAGAAGGTAACTTCTTTGT+GGG | - | chr4.2:51692369-51692388 | MS.gene061329:CDS | 35.0% | |
| GAAGCAGACAAGTGTAATTC+TGG | - | chr4.2:51692292-51692311 | MS.gene061329:CDS | 40.0% | |
| CCAGAAAGGATGGAAAGAAG+GGG | - | chr4.2:51692405-51692424 | MS.gene061329:CDS | 45.0% | |
| CTGAGCTACTAATGCCAACT+CGG | + | chr4.2:51692350-51692369 | None:intergenic | 45.0% | |
| GCCAGAAAGGATGGAAAGAA+GGG | - | chr4.2:51692404-51692423 | MS.gene061329:CDS | 45.0% | |
| GGCATTAGTAGCTCAGAAGA+AGG | - | chr4.2:51692354-51692373 | MS.gene061329:CDS | 45.0% | |
| GGTCGCATCTCAGTATCAAA+AGG | - | chr4.2:51692270-51692289 | MS.gene061329:CDS | 45.0% | |
| CCCCTTCTTTCCATCCTTTC+TGG | + | chr4.2:51692408-51692427 | None:intergenic | 50.0% | |
| CGCCAGAAAGGATGGAAAGA+AGG | - | chr4.2:51692403-51692422 | MS.gene061329:CDS | 50.0% | |
| GGCTCTGCTTGAAGCAAAGA+AGG | - | chr4.2:51692249-51692268 | MS.gene061329:CDS | 50.0% | |
| TGGTATGGAAACGTGTGAGC+AGG | - | chr4.2:51692312-51692331 | MS.gene061329:CDS | 50.0% | |
| GGAAACGTGTGAGCAGGCAA+GGG | - | chr4.2:51692318-51692337 | MS.gene061329:CDS | 55.0% | |
| TGGAAACGTGTGAGCAGGCA+AGG | - | chr4.2:51692317-51692336 | MS.gene061329:CDS | 55.0% | |
| ! | TCCATCCTTTCTGGCGAGCT+CGG | + | chr4.2:51692399-51692418 | None:intergenic | 55.0% |
| GAACTCCGAGCTCGCCAGAA+AGG | - | chr4.2:51692391-51692410 | MS.gene061329:CDS | 60.0% | |
| GGCAAGGGAGAAAGCCGAGT+TGG | - | chr4.2:51692333-51692352 | MS.gene061329:CDS | 60.0% | |
| TCCGAGCTCGCCAGAAAGGA+TGG | - | chr4.2:51692395-51692414 | MS.gene061329:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 51692247 | 51692459 | 51692247 | ID=MS.gene061329 |
| chr4.2 | mRNA | 51692247 | 51692459 | 51692247 | ID=MS.gene061329.t1;Parent=MS.gene061329 |
| chr4.2 | exon | 51692247 | 51692459 | 51692247 | ID=MS.gene061329.t1.exon1;Parent=MS.gene061329.t1 |
| chr4.2 | CDS | 51692247 | 51692459 | 51692247 | ID=cds.MS.gene061329.t1;Parent=MS.gene061329.t1 |
| Gene Sequence |
| Protein sequence |
