Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061542.t1 | XP_024635654.1 | 86.9 | 130 | 14 | 1 | 1 | 127 | 113 | 242 | 7.00E-56 | 226.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061542.t1 | Q9FJK3 | 41.5 | 65 | 38 | 0 | 1 | 65 | 113 | 177 | 2.7e-08 | 59.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061542.t1 | G7J8R0 | 86.9 | 130 | 14 | 1 | 1 | 127 | 113 | 242 | 5.1e-56 | 226.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04997 | MS.gene061542 | 0.839047 | 2.00E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061542.t1 | MTR_3g065100 | 93.077 | 130 | 6 | 1 | 1 | 127 | 113 | 242 | 1.84e-77 | 229 |
| MS.gene061542.t1 | MTR_8g036130 | 46.094 | 128 | 60 | 5 | 4 | 123 | 118 | 244 | 2.19e-20 | 83.6 |
| MS.gene061542.t1 | MTR_3g466980 | 37.984 | 129 | 53 | 4 | 1 | 122 | 113 | 221 | 4.01e-16 | 71.6 |
| MS.gene061542.t1 | MTR_4g032620 | 38.710 | 124 | 67 | 4 | 1 | 123 | 113 | 228 | 1.28e-15 | 70.5 |
| MS.gene061542.t1 | MTR_2g035590 | 53.061 | 49 | 22 | 1 | 1 | 48 | 40 | 88 | 1.04e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061542.t1 | AT5G48670 | 38.636 | 88 | 43 | 2 | 1 | 77 | 113 | 200 | 3.85e-12 | 62.0 |
Find 36 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCCATTCTCTTCAATCTT+AGG | 0.274749 | 3.2:-43855483 | None:intergenic |
| GATTTGGCCAAAACCCATTT+GGG | 0.290435 | 3.2:-43855629 | None:intergenic |
| TTTATGAACTTGATGAATAA+TGG | 0.322309 | 3.2:+43855567 | MS.gene061542:CDS |
| ATGCTTATATTGTGCATAAC+TGG | 0.326699 | 3.2:-43855318 | None:intergenic |
| CATGGTTGATTTGAATGATA+TGG | 0.333296 | 3.2:+43855338 | MS.gene061542:CDS |
| GAGTCATGGTCAACATGTTT+TGG | 0.338044 | 3.2:+43855509 | MS.gene061542:CDS |
| GGATTTGGCCAAAACCCATT+TGG | 0.342692 | 3.2:-43855630 | None:intergenic |
| TGGGGATCAGGCACCTCCTA+TGG | 0.395072 | 3.2:+43855593 | MS.gene061542:CDS |
| GAATAATGGTGGTGGGGATC+AGG | 0.411629 | 3.2:+43855581 | MS.gene061542:CDS |
| AATGGTCGTAGCACCAACTA+TGG | 0.416139 | 3.2:+43855446 | MS.gene061542:CDS |
| AGTATTTGAAAGATATCAAT+AGG | 0.431729 | 3.2:+43855376 | MS.gene061542:CDS |
| GCACCAACTATGGTGGCTAA+TGG | 0.492096 | 3.2:+43855456 | MS.gene061542:CDS |
| GGAGGGTTGAAATGTTGAGC+AGG | 0.493102 | 3.2:+43855397 | MS.gene061542:CDS |
| CTACCTAAGATTGAAGAGAA+TGG | 0.512642 | 3.2:+43855480 | MS.gene061542:CDS |
| AGGTCAAGGTCAAACTCAAA+TGG | 0.513312 | 3.2:+43855428 | MS.gene061542:CDS |
| GTTGAAATGTTGAGCAGGAA+TGG | 0.522645 | 3.2:+43855402 | MS.gene061542:CDS |
| GGGGATCAGGCACCTCCTAT+GGG | 0.527561 | 3.2:+43855594 | MS.gene061542:CDS |
| AGTCCATTAGCCACCATAGT+TGG | 0.539716 | 3.2:-43855459 | None:intergenic |
| TTTGAAAGATATCAATAGGA+GGG | 0.549226 | 3.2:+43855380 | MS.gene061542:CDS |
| AACTTGATGAATAATGGTGG+TGG | 0.552012 | 3.2:+43855573 | MS.gene061542:CDS |
| AGCAGGAATGGTCAAGGTCA+AGG | 0.553703 | 3.2:+43855414 | MS.gene061542:CDS |
| GAGAATGGACAAGGGAGTCA+TGG | 0.556512 | 3.2:+43855495 | MS.gene061542:CDS |
| GGATTGACATTTCCCATAGG+AGG | 0.571905 | 3.2:-43855606 | None:intergenic |
| ATTTGGCCAAAACCCATTTG+GGG | 0.587386 | 3.2:-43855628 | None:intergenic |
| TTTGGCCAAAACCCATTTGG+GGG | 0.595235 | 3.2:-43855627 | None:intergenic |
| ATTTGAAAGATATCAATAGG+AGG | 0.602830 | 3.2:+43855379 | MS.gene061542:CDS |
| GCATAACTGGATCAGCACTA+AGG | 0.605501 | 3.2:-43855305 | None:intergenic |
| AAGATTGAAGAGAATGGACA+AGG | 0.608283 | 3.2:+43855486 | MS.gene061542:CDS |
| ACTTGATGAATAATGGTGGT+GGG | 0.616834 | 3.2:+43855574 | MS.gene061542:CDS |
| ATGAACTTGATGAATAATGG+TGG | 0.624781 | 3.2:+43855570 | MS.gene061542:CDS |
| CTTGATGAATAATGGTGGTG+GGG | 0.636670 | 3.2:+43855575 | MS.gene061542:CDS |
| AGTTATGCACAATATAAGCA+TGG | 0.643634 | 3.2:+43855320 | MS.gene061542:CDS |
| TTGATTTGAATGATATGGCA+TGG | 0.644556 | 3.2:+43855343 | MS.gene061542:CDS |
| ATGTTGAGCAGGAATGGTCA+AGG | 0.649347 | 3.2:+43855408 | MS.gene061542:CDS |
| GGTCGTAGCACCAACTATGG+TGG | 0.652064 | 3.2:+43855449 | MS.gene061542:CDS |
| AGATTGAAGAGAATGGACAA+GGG | 0.691703 | 3.2:+43855487 | MS.gene061542:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTATTTGAAAGATATCAAT+AGG | + | chr3.2:43855376-43855395 | MS.gene061542:CDS | 20.0% |
| !! | TTTATGAACTTGATGAATAA+TGG | + | chr3.2:43855567-43855586 | MS.gene061542:CDS | 20.0% |
| ! | ATTTGAAAGATATCAATAGG+AGG | + | chr3.2:43855379-43855398 | MS.gene061542:CDS | 25.0% |
| ! | TTTGAAAGATATCAATAGGA+GGG | + | chr3.2:43855380-43855399 | MS.gene061542:CDS | 25.0% |
| AGTTATGCACAATATAAGCA+TGG | + | chr3.2:43855320-43855339 | MS.gene061542:CDS | 30.0% | |
| ATGAACTTGATGAATAATGG+TGG | + | chr3.2:43855570-43855589 | MS.gene061542:CDS | 30.0% | |
| ATGCTTATATTGTGCATAAC+TGG | - | chr3.2:43855321-43855340 | None:intergenic | 30.0% | |
| ! | TTGATTTGAATGATATGGCA+TGG | + | chr3.2:43855343-43855362 | MS.gene061542:CDS | 30.0% |
| !! | CATGGTTGATTTGAATGATA+TGG | + | chr3.2:43855338-43855357 | MS.gene061542:CDS | 30.0% |
| AACTTGATGAATAATGGTGG+TGG | + | chr3.2:43855573-43855592 | MS.gene061542:CDS | 35.0% | |
| AAGATTGAAGAGAATGGACA+AGG | + | chr3.2:43855486-43855505 | MS.gene061542:CDS | 35.0% | |
| ACTTGATGAATAATGGTGGT+GGG | + | chr3.2:43855574-43855593 | MS.gene061542:CDS | 35.0% | |
| AGATTGAAGAGAATGGACAA+GGG | + | chr3.2:43855487-43855506 | MS.gene061542:CDS | 35.0% | |
| CTACCTAAGATTGAAGAGAA+TGG | + | chr3.2:43855480-43855499 | MS.gene061542:CDS | 35.0% | |
| TGTCCATTCTCTTCAATCTT+AGG | - | chr3.2:43855486-43855505 | None:intergenic | 35.0% | |
| !! | ACATGTTTTGGACATGAACA+TGG | + | chr3.2:43855521-43855540 | MS.gene061542:CDS | 35.0% |
| AGGTCAAGGTCAAACTCAAA+TGG | + | chr3.2:43855428-43855447 | MS.gene061542:CDS | 40.0% | |
| ATTTGGCCAAAACCCATTTG+GGG | - | chr3.2:43855631-43855650 | None:intergenic | 40.0% | |
| GAAATGTCAATCCCCCAAAT+GGG | + | chr3.2:43855616-43855635 | MS.gene061542:CDS | 40.0% | |
| GATTTGGCCAAAACCCATTT+GGG | - | chr3.2:43855632-43855651 | None:intergenic | 40.0% | |
| GTTGAAATGTTGAGCAGGAA+TGG | + | chr3.2:43855402-43855421 | MS.gene061542:CDS | 40.0% | |
| ! | CTTGATGAATAATGGTGGTG+GGG | + | chr3.2:43855575-43855594 | MS.gene061542:CDS | 40.0% |
| ! | GAGTCATGGTCAACATGTTT+TGG | + | chr3.2:43855509-43855528 | MS.gene061542:CDS | 40.0% |
| AATGGTCGTAGCACCAACTA+TGG | + | chr3.2:43855446-43855465 | MS.gene061542:CDS | 45.0% | |
| AGTCCATTAGCCACCATAGT+TGG | - | chr3.2:43855462-43855481 | None:intergenic | 45.0% | |
| ATGTTGAGCAGGAATGGTCA+AGG | + | chr3.2:43855408-43855427 | MS.gene061542:CDS | 45.0% | |
| GCATAACTGGATCAGCACTA+AGG | - | chr3.2:43855308-43855327 | None:intergenic | 45.0% | |
| GGAAATGTCAATCCCCCAAA+TGG | + | chr3.2:43855615-43855634 | MS.gene061542:CDS | 45.0% | |
| GGATTGACATTTCCCATAGG+AGG | - | chr3.2:43855609-43855628 | None:intergenic | 45.0% | |
| GGATTTGGCCAAAACCCATT+TGG | - | chr3.2:43855633-43855652 | None:intergenic | 45.0% | |
| TTTGGCCAAAACCCATTTGG+GGG | - | chr3.2:43855630-43855649 | None:intergenic | 45.0% | |
| !! | TCAATCCCCCAAATGGGTTT+TGG | + | chr3.2:43855622-43855641 | MS.gene061542:CDS | 45.0% |
| AGCAGGAATGGTCAAGGTCA+AGG | + | chr3.2:43855414-43855433 | MS.gene061542:CDS | 50.0% | |
| GAGAATGGACAAGGGAGTCA+TGG | + | chr3.2:43855495-43855514 | MS.gene061542:CDS | 50.0% | |
| GCACCAACTATGGTGGCTAA+TGG | + | chr3.2:43855456-43855475 | MS.gene061542:CDS | 50.0% | |
| GGAGGGTTGAAATGTTGAGC+AGG | + | chr3.2:43855397-43855416 | MS.gene061542:CDS | 50.0% | |
| GGGGGATTGACATTTCCCAT+AGG | - | chr3.2:43855612-43855631 | None:intergenic | 50.0% | |
| !! | GAATAATGGTGGTGGGGATC+AGG | + | chr3.2:43855581-43855600 | MS.gene061542:CDS | 50.0% |
| GGTCGTAGCACCAACTATGG+TGG | + | chr3.2:43855449-43855468 | MS.gene061542:CDS | 55.0% | |
| GGGGATCAGGCACCTCCTAT+GGG | + | chr3.2:43855594-43855613 | MS.gene061542:CDS | 60.0% | |
| TGGGGATCAGGCACCTCCTA+TGG | + | chr3.2:43855593-43855612 | MS.gene061542:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 43855282 | 43855665 | 43855282 | ID=MS.gene061542 |
| chr3.2 | mRNA | 43855282 | 43855665 | 43855282 | ID=MS.gene061542.t1;Parent=MS.gene061542 |
| chr3.2 | exon | 43855282 | 43855665 | 43855282 | ID=MS.gene061542.t1.exon1;Parent=MS.gene061542.t1 |
| chr3.2 | CDS | 43855282 | 43855665 | 43855282 | ID=cds.MS.gene061542.t1;Parent=MS.gene061542.t1 |
| Gene Sequence |
| Protein sequence |
