Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061743.t1 | XP_013457933.1 | 97.8 | 324 | 7 | 0 | 1 | 324 | 1 | 324 | 2.30E-167 | 598.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061743.t1 | A0A072UQ71 | 97.8 | 324 | 7 | 0 | 1 | 324 | 1 | 324 | 1.6e-167 | 598.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050649 | MS.gene061743 | 0.82847 | 8.83E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061743.t1 | MTR_4g109020 | 97.840 | 324 | 7 | 0 | 1 | 324 | 1 | 324 | 0.0 | 631 |
| MS.gene061743.t1 | MTR_3g037110 | 69.394 | 330 | 89 | 3 | 1 | 324 | 1 | 324 | 1.36e-153 | 433 |
| MS.gene061743.t1 | MTR_4g082350 | 28.082 | 292 | 198 | 9 | 6 | 294 | 49 | 331 | 4.53e-28 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061743.t1 | AT5G61340 | 46.687 | 332 | 157 | 8 | 1 | 323 | 5 | 325 | 6.26e-81 | 249 |
| MS.gene061743.t1 | AT5G61340 | 46.687 | 332 | 157 | 8 | 1 | 323 | 38 | 358 | 2.09e-80 | 249 |
| MS.gene061743.t1 | AT1G69430 | 29.667 | 300 | 183 | 10 | 6 | 294 | 45 | 327 | 2.71e-24 | 101 |
| MS.gene061743.t1 | AT1G26650 | 27.526 | 287 | 196 | 8 | 6 | 289 | 29 | 306 | 8.02e-24 | 100 |
Find 55 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAAATTTATAACCGTTTA+AGG | 0.015328 | 8.1:-16435610 | MS.gene061743:CDS |
| TTTCAAGAAGTGGACAATTA+TGG | 0.284116 | 8.1:-16434849 | MS.gene061743:CDS |
| GAGTTTAAGTGCTTGAATTA+TGG | 0.313893 | 8.1:+16435451 | None:intergenic |
| TAAAGCTTGTATCTTGATAA+GGG | 0.315641 | 8.1:-16435123 | MS.gene061743:CDS |
| TTAAAGCTTGTATCTTGATA+AGG | 0.341052 | 8.1:-16435124 | MS.gene061743:CDS |
| AAGTGAAATAATGATAGTTT+TGG | 0.354262 | 8.1:+16435717 | None:intergenic |
| CAAGAAACAGTTGCAGATAT+AGG | 0.364089 | 8.1:+16435358 | None:intergenic |
| CTCCTCTTCATGTCAGCGAC+CGG | 0.364905 | 8.1:-16435248 | MS.gene061743:CDS |
| AATATGTCACTTGTTCTATC+TGG | 0.371364 | 8.1:-16435179 | MS.gene061743:CDS |
| GCAGCCAAGGCAATATTAAC+AGG | 0.394585 | 8.1:+16435068 | None:intergenic |
| GGTTATAAATTTGAGGCAAA+AGG | 0.402196 | 8.1:+16435618 | None:intergenic |
| TCCTCTTCATGTCAGCGACC+GGG | 0.410312 | 8.1:-16435247 | MS.gene061743:CDS |
| TGTTTCACTTCAGAATTGTA+AGG | 0.417611 | 8.1:-16435034 | MS.gene061743:CDS |
| GTAAGGGAATATCACATTGT+TGG | 0.423368 | 8.1:-16435017 | MS.gene061743:CDS |
| AATGTTGAACAATAGAGAAT+AGG | 0.432290 | 8.1:+16435559 | None:intergenic |
| CTTAAACGGTTATAAATTTG+AGG | 0.435128 | 8.1:+16435611 | None:intergenic |
| ATCCCCTCTAAAAGCACAAA+AGG | 0.440963 | 8.1:+16434984 | None:intergenic |
| ACTTCCTGTTAATATTGCCT+TGG | 0.454147 | 8.1:-16435072 | MS.gene061743:CDS |
| AGAAGGACAGTTAGTGTGAA+AGG | 0.454408 | 8.1:+16435488 | None:intergenic |
| GAGAATGAAGGTGGCAAAGA+TGG | 0.476193 | 8.1:+16435416 | None:intergenic |
| TTCAGCTTTGTTGAAACACT+TGG | 0.481261 | 8.1:-16435290 | MS.gene061743:CDS |
| GACATGAAGAGGAGGAAACT+AGG | 0.482045 | 8.1:+16435257 | None:intergenic |
| GTCACTTGTTCTATCTGGTA+TGG | 0.482263 | 8.1:-16435174 | MS.gene061743:CDS |
| GTTATAAATTTGAGGCAAAA+GGG | 0.486260 | 8.1:+16435619 | None:intergenic |
| AGGAGAATCATGCATTGGTC+AGG | 0.492954 | 8.1:-16434883 | MS.gene061743:CDS |
| ATGAGTATCGAAGCAGAGAA+AGG | 0.504345 | 8.1:+16435671 | None:intergenic |
| ATTTGAGGCAAAAGGGATGA+AGG | 0.511390 | 8.1:+16435626 | None:intergenic |
| CCTCTTCATGTCAGCGACCG+GGG | 0.524558 | 8.1:-16435246 | MS.gene061743:CDS |
| GGAATATCACATTGTTGGAA+AGG | 0.524960 | 8.1:-16435012 | MS.gene061743:CDS |
| GTGGACAATTATGGTTACAT+TGG | 0.525805 | 8.1:-16434840 | MS.gene061743:CDS |
| CAGATATAGGTGTGGAAGAG+TGG | 0.530195 | 8.1:+16435371 | None:intergenic |
| CAAGGCTTCAATAGCAGCCA+AGG | 0.531315 | 8.1:+16435055 | None:intergenic |
| GAAGGACAGTTAGTGTGAAA+GGG | 0.531755 | 8.1:+16435489 | None:intergenic |
| GGAAGGACATGGTGGATACT+CGG | 0.533045 | 8.1:-16435153 | MS.gene061743:CDS |
| GTTCAACATTCTTAACCTCA+AGG | 0.536846 | 8.1:-16435546 | MS.gene061743:CDS |
| CCCCGGTCGCTGACATGAAG+AGG | 0.552022 | 8.1:+16435246 | None:intergenic |
| GTTTCACTTCAGAATTGTAA+GGG | 0.558550 | 8.1:-16435033 | MS.gene061743:CDS |
| GCTATTATCGAAGAGAATGA+AGG | 0.569442 | 8.1:+16435404 | None:intergenic |
| ATTGCATATCACAAGTGCAT+TGG | 0.569840 | 8.1:+16435199 | None:intergenic |
| CTTGTTCTATCTGGTATGGA+AGG | 0.576428 | 8.1:-16435170 | MS.gene061743:CDS |
| AGAATCATGCATTGGTCAGG+AGG | 0.582085 | 8.1:-16434880 | MS.gene061743:CDS |
| GATAAGGGGAAAGACATCAA+TGG | 0.595318 | 8.1:-16435108 | MS.gene061743:CDS |
| TTGAACAATAGAGAATAGGA+AGG | 0.596055 | 8.1:+16435563 | None:intergenic |
| AAGACAGGAGAATCATGCAT+TGG | 0.601882 | 8.1:-16434888 | MS.gene061743:CDS |
| AAAGCTTGTATCTTGATAAG+GGG | 0.603714 | 8.1:-16435122 | MS.gene061743:CDS |
| TACAATTCTGAAGTGAAACA+AGG | 0.613545 | 8.1:+16435037 | None:intergenic |
| ATCACATTGTTGGAAAGGCA+AGG | 0.615036 | 8.1:-16435007 | MS.gene061743:CDS |
| GGCAAAAGGGATGAAGGAGA+AGG | 0.617930 | 8.1:+16435632 | None:intergenic |
| AACAGTTGCAGATATAGGTG+TGG | 0.621021 | 8.1:+16435363 | None:intergenic |
| AGCAACTCACAATTGTGTCA+AGG | 0.625192 | 8.1:+16434928 | None:intergenic |
| TGATTGTTTGAGAAACCTTG+AGG | 0.628286 | 8.1:+16435531 | None:intergenic |
| CGGTCGCTGACATGAAGAGG+AGG | 0.647162 | 8.1:+16435249 | None:intergenic |
| CTATCTGGTATGGAAGGACA+TGG | 0.647711 | 8.1:-16435164 | MS.gene061743:CDS |
| ATTATCGAAGAGAATGAAGG+TGG | 0.661031 | 8.1:+16435407 | None:intergenic |
| TCTGGTATGGAAGGACATGG+TGG | 0.680756 | 8.1:-16435161 | MS.gene061743:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTTTTTGTATTATGTTTT+TGG | - | chr8.1:16435261-16435280 | MS.gene061743:CDS | 15.0% |
| !!! | AAGTGAAATAATGATAGTTT+TGG | + | chr8.1:16434862-16434881 | None:intergenic | 20.0% |
| ! | AATGTTGAACAATAGAGAAT+AGG | + | chr8.1:16435020-16435039 | None:intergenic | 25.0% |
| ! | CTCAAATTTATAACCGTTTA+AGG | - | chr8.1:16434966-16434985 | MS.gene061743:CDS | 25.0% |
| ! | CTTAAACGGTTATAAATTTG+AGG | + | chr8.1:16434968-16434987 | None:intergenic | 25.0% |
| ! | TAAAGCTTGTATCTTGATAA+GGG | - | chr8.1:16435453-16435472 | MS.gene061743:CDS | 25.0% |
| ! | TTAAAGCTTGTATCTTGATA+AGG | - | chr8.1:16435452-16435471 | MS.gene061743:CDS | 25.0% |
| !! | GTTATAAATTTGAGGCAAAA+GGG | + | chr8.1:16434960-16434979 | None:intergenic | 25.0% |
| AAAGCTTGTATCTTGATAAG+GGG | - | chr8.1:16435454-16435473 | MS.gene061743:CDS | 30.0% | |
| CATCAAAAAGAGTCCTTAAA+CGG | + | chr8.1:16434982-16435001 | None:intergenic | 30.0% | |
| GAGTTTAAGTGCTTGAATTA+TGG | + | chr8.1:16435128-16435147 | None:intergenic | 30.0% | |
| GTTTCACTTCAGAATTGTAA+GGG | - | chr8.1:16435543-16435562 | MS.gene061743:CDS | 30.0% | |
| TGTTTCACTTCAGAATTGTA+AGG | - | chr8.1:16435542-16435561 | MS.gene061743:CDS | 30.0% | |
| TTGAACAATAGAGAATAGGA+AGG | + | chr8.1:16435016-16435035 | None:intergenic | 30.0% | |
| TTTCAAGAAGTGGACAATTA+TGG | - | chr8.1:16435727-16435746 | MS.gene061743:CDS | 30.0% | |
| ! | AATATGTCACTTGTTCTATC+TGG | - | chr8.1:16435397-16435416 | MS.gene061743:CDS | 30.0% |
| ! | GGTTATAAATTTGAGGCAAA+AGG | + | chr8.1:16434961-16434980 | None:intergenic | 30.0% |
| ! | TTTTACAAAAGCTTCAAGAC+AGG | - | chr8.1:16435673-16435692 | MS.gene061743:CDS | 30.0% |
| !! | TACAATTCTGAAGTGAAACA+AGG | + | chr8.1:16435542-16435561 | None:intergenic | 30.0% |
| ACTTCCTGTTAATATTGCCT+TGG | - | chr8.1:16435504-16435523 | MS.gene061743:CDS | 35.0% | |
| ATTATCGAAGAGAATGAAGG+TGG | + | chr8.1:16435172-16435191 | None:intergenic | 35.0% | |
| ATTGCATATCACAAGTGCAT+TGG | + | chr8.1:16435380-16435399 | None:intergenic | 35.0% | |
| CAAGAAACAGTTGCAGATAT+AGG | + | chr8.1:16435221-16435240 | None:intergenic | 35.0% | |
| GCTATTATCGAAGAGAATGA+AGG | + | chr8.1:16435175-16435194 | None:intergenic | 35.0% | |
| GGAATATCACATTGTTGGAA+AGG | - | chr8.1:16435564-16435583 | MS.gene061743:CDS | 35.0% | |
| GTAAGGGAATATCACATTGT+TGG | - | chr8.1:16435559-16435578 | MS.gene061743:CDS | 35.0% | |
| GTGGACAATTATGGTTACAT+TGG | - | chr8.1:16435736-16435755 | MS.gene061743:CDS | 35.0% | |
| GTTCAACATTCTTAACCTCA+AGG | - | chr8.1:16435030-16435049 | MS.gene061743:CDS | 35.0% | |
| TGATTGTTTGAGAAACCTTG+AGG | + | chr8.1:16435048-16435067 | None:intergenic | 35.0% | |
| !! | GGACAAACATTTTCAAGAAG+TGG | - | chr8.1:16435717-16435736 | MS.gene061743:CDS | 35.0% |
| !! | TTCAGCTTTGTTGAAACACT+TGG | - | chr8.1:16435286-16435305 | MS.gene061743:CDS | 35.0% |
| AACAGTTGCAGATATAGGTG+TGG | + | chr8.1:16435216-16435235 | None:intergenic | 40.0% | |
| AAGACAGGAGAATCATGCAT+TGG | - | chr8.1:16435688-16435707 | MS.gene061743:CDS | 40.0% | |
| AGAAGGACAGTTAGTGTGAA+AGG | + | chr8.1:16435091-16435110 | None:intergenic | 40.0% | |
| AGCAACTCACAATTGTGTCA+AGG | + | chr8.1:16435651-16435670 | None:intergenic | 40.0% | |
| ATCACATTGTTGGAAAGGCA+AGG | - | chr8.1:16435569-16435588 | MS.gene061743:CDS | 40.0% | |
| CTTGTTCTATCTGGTATGGA+AGG | - | chr8.1:16435406-16435425 | MS.gene061743:CDS | 40.0% | |
| GAAGGACAGTTAGTGTGAAA+GGG | + | chr8.1:16435090-16435109 | None:intergenic | 40.0% | |
| GATAAGGGGAAAGACATCAA+TGG | - | chr8.1:16435468-16435487 | MS.gene061743:CDS | 40.0% | |
| GTCACTTGTTCTATCTGGTA+TGG | - | chr8.1:16435402-16435421 | MS.gene061743:CDS | 40.0% | |
| ! | AAGGCCTTTTGTGCTTTTAG+AGG | - | chr8.1:16435588-16435607 | MS.gene061743:CDS | 40.0% |
| ! | AGGCCTTTTGTGCTTTTAGA+GGG | - | chr8.1:16435589-16435608 | MS.gene061743:CDS | 40.0% |
| ! | ATCCCCTCTAAAAGCACAAA+AGG | + | chr8.1:16435595-16435614 | None:intergenic | 40.0% |
| ! | ATTTGAGGCAAAAGGGATGA+AGG | + | chr8.1:16434953-16434972 | None:intergenic | 40.0% |
| ! | TGGATGCTTTTGCAATCAGA+AGG | + | chr8.1:16435108-16435127 | None:intergenic | 40.0% |
| !! | ATGAGTATCGAAGCAGAGAA+AGG | + | chr8.1:16434908-16434927 | None:intergenic | 40.0% |
| CACAGAAAAAAGAACTGCCC+CGG | + | chr8.1:16435350-16435369 | None:intergenic | 45.0% | |
| CAGATATAGGTGTGGAAGAG+TGG | + | chr8.1:16435208-16435227 | None:intergenic | 45.0% | |
| CTATCTGGTATGGAAGGACA+TGG | - | chr8.1:16435412-16435431 | MS.gene061743:CDS | 45.0% | |
| GACATGAAGAGGAGGAAACT+AGG | + | chr8.1:16435322-16435341 | None:intergenic | 45.0% | |
| GAGAATGAAGGTGGCAAAGA+TGG | + | chr8.1:16435163-16435182 | None:intergenic | 45.0% | |
| GCAGCCAAGGCAATATTAAC+AGG | + | chr8.1:16435511-16435530 | None:intergenic | 45.0% | |
| ! | AGAATCATGCATTGGTCAGG+AGG | - | chr8.1:16435696-16435715 | MS.gene061743:CDS | 45.0% |
| ! | AGGACTCTTTTTGATGCTGC+AGG | - | chr8.1:16434986-16435005 | MS.gene061743:CDS | 45.0% |
| ! | AGGAGAATCATGCATTGGTC+AGG | - | chr8.1:16435693-16435712 | MS.gene061743:CDS | 45.0% |
| !! | GGCCTTTTGTGCTTTTAGAG+GGG | - | chr8.1:16435590-16435609 | MS.gene061743:CDS | 45.0% |
| CAAGGCTTCAATAGCAGCCA+AGG | + | chr8.1:16435524-16435543 | None:intergenic | 50.0% | |
| GGCAAAAGGGATGAAGGAGA+AGG | + | chr8.1:16434947-16434966 | None:intergenic | 50.0% | |
| TCTGGTATGGAAGGACATGG+TGG | - | chr8.1:16435415-16435434 | MS.gene061743:CDS | 50.0% | |
| !! | GGAAGGACATGGTGGATACT+CGG | - | chr8.1:16435423-16435442 | MS.gene061743:CDS | 50.0% |
| CTCCTCTTCATGTCAGCGAC+CGG | - | chr8.1:16435328-16435347 | MS.gene061743:CDS | 55.0% | |
| TCCTCTTCATGTCAGCGACC+GGG | - | chr8.1:16435329-16435348 | MS.gene061743:CDS | 55.0% | |
| CCTCTTCATGTCAGCGACCG+GGG | - | chr8.1:16435330-16435349 | MS.gene061743:CDS | 60.0% | |
| CGGTCGCTGACATGAAGAGG+AGG | + | chr8.1:16435330-16435349 | None:intergenic | 60.0% | |
| CCCCGGTCGCTGACATGAAG+AGG | + | chr8.1:16435333-16435352 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 16434812 | 16435786 | 16434812 | ID=MS.gene061743 |
| chr8.1 | mRNA | 16434812 | 16435786 | 16434812 | ID=MS.gene061743.t1;Parent=MS.gene061743 |
| chr8.1 | exon | 16434812 | 16435786 | 16434812 | ID=MS.gene061743.t1.exon1;Parent=MS.gene061743.t1 |
| chr8.1 | CDS | 16434812 | 16435786 | 16434812 | ID=cds.MS.gene061743.t1;Parent=MS.gene061743.t1 |
| Gene Sequence |
| Protein sequence |
