Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061772.t1 | RHN40044.1 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 3.70E-100 | 374.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061772.t1 | Q7M443 | 35.0 | 274 | 151 | 9 | 14 | 280 | 2 | 255 | 6.3e-32 | 139.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061772.t1 | A0A396GKD7 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 2.7e-100 | 374.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049681 | MS.gene061772 | 0.80321 | 3.89E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene061772 | 0.831249 | 1.86E-55 | -1.69E-46 |
| MS.gene054183 | MS.gene061772 | 0.81275 | 3.62E-51 | -1.69E-46 |
| MS.gene059294 | MS.gene061772 | 0.817158 | 3.81E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061772.t1 | MTR_0002s0040 | 53.698 | 311 | 103 | 10 | 1 | 290 | 1 | 291 | 2.50e-95 | 283 |
| MS.gene061772.t1 | MTR_0263s0020 | 56.164 | 292 | 96 | 9 | 1 | 280 | 1 | 272 | 4.21e-94 | 280 |
| MS.gene061772.t1 | MTR_1457s0010 | 66.234 | 231 | 54 | 8 | 65 | 291 | 1 | 211 | 2.88e-93 | 275 |
| MS.gene061772.t1 | MTR_0002s1060 | 51.546 | 291 | 108 | 11 | 4 | 280 | 6 | 277 | 3.70e-75 | 232 |
| MS.gene061772.t1 | MTR_0062s0020 | 38.542 | 288 | 140 | 8 | 14 | 280 | 4 | 275 | 1.39e-48 | 164 |
| MS.gene061772.t1 | MTR_0062s0090 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene061772.t1 | MTR_0062s0040 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene061772.t1 | MTR_0062s0060 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene061772.t1 | MTR_0443s0040 | 39.175 | 291 | 132 | 12 | 14 | 280 | 16 | 285 | 1.26e-47 | 161 |
| MS.gene061772.t1 | MTR_0062s0170 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_0062s0110 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_0062s0130 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_2g036240 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_2g036260 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_2g036290 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_8g046730 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene061772.t1 | MTR_6g079630 | 34.182 | 275 | 156 | 8 | 12 | 280 | 32 | 287 | 3.22e-37 | 134 |
| MS.gene061772.t1 | MTR_2g096030 | 32.990 | 291 | 163 | 10 | 14 | 291 | 31 | 302 | 1.39e-33 | 124 |
| MS.gene061772.t1 | MTR_2g096040 | 33.202 | 253 | 145 | 6 | 39 | 283 | 26 | 262 | 2.08e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | - | 57512:15132-15151 | MS.gene061772:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | - | 57512:15137-15156 | MS.gene061772:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | - | 57512:15138-15157 | MS.gene061772:intergenic | 25.0% |
| !! | TTCATTTTACGAGAATACAT+TGG | + | 57512:15099-15118 | MS.gene061772:CDS | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | - | 57512:15391-15410 | MS.gene061772:intergenic | 30.0% | |
| ATTTCGTTGTAGTCAATATC+AGG | - | 57512:15156-15175 | MS.gene061772:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | - | 57512:15392-15411 | MS.gene061772:intergenic | 30.0% | |
| TATAAAAAGACTCAAGGAAG+AGG | + | 57512:15554-15573 | MS.gene061772:CDS | 30.0% | |
| TTTCGTTGTAGTCAATATCA+GGG | - | 57512:15155-15174 | MS.gene061772:intergenic | 30.0% | |
| ! | GTAGTGTTCATTAGTTTCAT+GGG | - | 57512:15610-15629 | MS.gene061772:intergenic | 30.0% |
| ! | TGTAGTGTTCATTAGTTTCA+TGG | - | 57512:15611-15630 | MS.gene061772:intergenic | 30.0% |
| !! | AATCGATTTCCATGTCATTT+TGG | + | 57512:15173-15192 | MS.gene061772:CDS | 30.0% |
| !! | ACTTTTGAAAAACTCCTAGT+AGG | + | 57512:15759-15778 | MS.gene061772:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | - | 57512:15405-15424 | MS.gene061772:intergenic | 30.0% |
| AAGATTTCTCCAACTACGTT+GGG | + | 57512:15526-15545 | MS.gene061772:CDS | 35.0% | |
| ACCACAGTGACGATATTAAT+TGG | + | 57512:15646-15665 | MS.gene061772:CDS | 35.0% | |
| ACTTACAACGAAGATGGAAA+AGG | + | 57512:15210-15229 | MS.gene061772:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | - | 57512:15205-15224 | MS.gene061772:intergenic | 35.0% | |
| CCACAGTGACGATATTAATT+GGG | + | 57512:15647-15666 | MS.gene061772:CDS | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | + | 57512:15409-15428 | MS.gene061772:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | - | 57512:15390-15409 | MS.gene061772:intergenic | 35.0% | |
| GGACGTTATAAAAAGACTCA+AGG | + | 57512:15548-15567 | MS.gene061772:CDS | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | - | 57512:15393-15412 | MS.gene061772:intergenic | 35.0% | |
| GTTGGAGAAATCTTCTTCAT+TGG | - | 57512:15520-15539 | MS.gene061772:intergenic | 35.0% | |
| ! | TGTTCATTAGTTTCATGGGA+TGG | - | 57512:15606-15625 | MS.gene061772:intergenic | 35.0% |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | - | 57512:15414-15433 | MS.gene061772:intergenic | 35.0% |
| AAAAGACTCAAGGAAGAGGT+AGG | + | 57512:15558-15577 | MS.gene061772:CDS | 40.0% | |
| AACACTACAAGCTACTCTAC+AGG | + | 57512:15622-15641 | MS.gene061772:CDS | 40.0% | |
| ACACTACAAGCTACTCTACA+GGG | + | 57512:15623-15642 | MS.gene061772:CDS | 40.0% | |
| AGATTTCTCCAACTACGTTG+GGG | + | 57512:15527-15546 | MS.gene061772:CDS | 40.0% | |
| ATCCAAGACTACACGTTTGA+TGG | + | 57512:15465-15484 | MS.gene061772:CDS | 40.0% | |
| CAGATATCAAGATCCCAATC+GGG | - | 57512:15252-15271 | MS.gene061772:intergenic | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | - | 57512:15206-15225 | MS.gene061772:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | + | 57512:15204-15223 | MS.gene061772:CDS | 40.0% | |
| CCCAATTAATATCGTCACTG+TGG | - | 57512:15650-15669 | MS.gene061772:intergenic | 40.0% | |
| CGGAAAATTCTATCCCGATT+GGG | + | 57512:15236-15255 | MS.gene061772:CDS | 40.0% | |
| GAAGATTTCTCCAACTACGT+TGG | + | 57512:15525-15544 | MS.gene061772:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | - | 57512:15082-15101 | MS.gene061772:intergenic | 40.0% | |
| GATCTATGTTAGACTCGCAA+GGG | + | 57512:15728-15747 | MS.gene061772:CDS | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | + | 57512:15252-15271 | MS.gene061772:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | - | 57512:15253-15272 | MS.gene061772:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | + | 57512:15293-15312 | MS.gene061772:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | + | 57512:15341-15360 | MS.gene061772:CDS | 40.0% | |
| TGATCTATGTTAGACTCGCA+AGG | + | 57512:15727-15746 | MS.gene061772:CDS | 40.0% | |
| TTGCGAGTCTAACATAGATC+AGG | - | 57512:15728-15747 | MS.gene061772:intergenic | 40.0% | |
| ! | AACGCAGTGGCATTTTCATT+TGG | + | 57512:15868-15887 | MS.gene061772:CDS | 40.0% |
| ! | CCAATCGGGATAGAATTTTC+CGG | - | 57512:15238-15257 | MS.gene061772:intergenic | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | - | 57512:15445-15464 | MS.gene061772:intergenic | 40.0% |
| ACAAAGTCAGTCTCGTCAGT+GGG | - | 57512:15702-15721 | MS.gene061772:intergenic | 45.0% | |
| ACGAAGACATCTAGTGGAAG+TGG | - | 57512:15819-15838 | MS.gene061772:intergenic | 45.0% | |
| AGTTTCATGGGATGGAGCAA+TGG | - | 57512:15598-15617 | MS.gene061772:intergenic | 45.0% | |
| CCGGAAAATTCTATCCCGAT+TGG | + | 57512:15235-15254 | MS.gene061772:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | - | 57512:15312-15331 | MS.gene061772:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | + | 57512:15359-15378 | MS.gene061772:CDS | 45.0% | |
| GCGTTGAACCTTCATGAGAT+CGG | - | 57512:15853-15872 | MS.gene061772:intergenic | 45.0% | |
| GGAAGAGGTAGGTATCAATG+TGG | + | 57512:15569-15588 | MS.gene061772:CDS | 45.0% | |
| GGTTAGGCTGCATGTAGAAT+TGG | - | 57512:15680-15699 | MS.gene061772:intergenic | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | + | 57512:15315-15334 | MS.gene061772:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | - | 57512:15199-15218 | MS.gene061772:intergenic | 45.0% | |
| TACAAAGTCAGTCTCGTCAG+TGG | - | 57512:15703-15722 | MS.gene061772:intergenic | 45.0% | |
| TATAACGTCCCCAACGTAGT+TGG | - | 57512:15538-15557 | MS.gene061772:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | - | 57512:15470-15489 | MS.gene061772:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | + | 57512:15216-15235 | MS.gene061772:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | + | 57512:15318-15337 | MS.gene061772:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | - | 57512:15282-15301 | MS.gene061772:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | - | 57512:15430-15449 | MS.gene061772:intergenic | 50.0% | |
| ATTACGTCCGTCACGTGGAT+CGG | - | 57512:15793-15812 | MS.gene061772:intergenic | 50.0% | |
| CAAAGTCAGTCTCGTCAGTG+GGG | - | 57512:15701-15720 | MS.gene061772:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | + | 57512:15302-15321 | MS.gene061772:CDS | 50.0% | |
| CTCATGAAGGTTCAACGCAG+TGG | + | 57512:15855-15874 | MS.gene061772:CDS | 50.0% | |
| CTCGCAACGAAGACATCTAG+TGG | - | 57512:15825-15844 | MS.gene061772:intergenic | 50.0% | |
| GAGTTGCTCCGATCTCATGA+AGG | + | 57512:15842-15861 | MS.gene061772:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | - | 57512:15313-15332 | MS.gene061772:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | + | 57512:15299-15318 | MS.gene061772:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | - | 57512:15185-15204 | MS.gene061772:intergenic | 50.0% | |
| GGAACATTACGTCCGTCACG+TGG | - | 57512:15798-15817 | MS.gene061772:intergenic | 55.0% | |
| TCAGTCTCGTCAGTGGGGTT+AGG | - | 57512:15696-15715 | MS.gene061772:intergenic | 55.0% | |
| ! | GATCGGTGCTTGCTCCTACT+AGG | - | 57512:15776-15795 | MS.gene061772:intergenic | 55.0% |
| CTGCGGTATACCACGTGTGC+AGG | + | 57512:15269-15288 | MS.gene061772:CDS | 60.0% | |
| !! | GCAAGCACCGATCCACGTGA+CGG | + | 57512:15783-15802 | MS.gene061772:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57512 | gene | 15063 | 15942 | 15063 | ID=MS.gene061772 |
| 57512 | mRNA | 15063 | 15942 | 15063 | ID=MS.gene061772.t1;Parent=MS.gene061772 |
| 57512 | exon | 15063 | 15942 | 15063 | ID=MS.gene061772.t1.exon1;Parent=MS.gene061772.t1 |
| 57512 | CDS | 15063 | 15942 | 15063 | ID=cds.MS.gene061772.t1;Parent=MS.gene061772.t1 |
| Gene Sequence |
| Protein sequence |
