Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061786.t1 | XP_003618301.1 | 92.4 | 172 | 13 | 0 | 1 | 172 | 1 | 172 | 2.10E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061786.t1 | G7KIS9 | 92.4 | 172 | 13 | 0 | 1 | 172 | 1 | 172 | 1.5e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene061786 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene058010 | MS.gene061786 | 0.819205 | 1.31E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061786.t1 | MTR_6g007360 | 91.908 | 173 | 14 | 0 | 1 | 173 | 1 | 173 | 3.31e-119 | 338 |
| MS.gene061786.t1 | MTR_1g115165 | 91.908 | 173 | 14 | 0 | 1 | 173 | 1 | 173 | 6.11e-119 | 337 |
| MS.gene061786.t1 | MTR_4g078180 | 92.169 | 166 | 13 | 0 | 1 | 166 | 1 | 166 | 6.56e-115 | 324 |
| MS.gene061786.t1 | MTR_7g446320 | 86.093 | 151 | 21 | 0 | 23 | 173 | 9 | 159 | 3.02e-94 | 274 |
| MS.gene061786.t1 | MTR_5g094775 | 91.150 | 113 | 10 | 0 | 47 | 159 | 2 | 114 | 7.24e-72 | 218 |
| MS.gene061786.t1 | MTR_3g022250 | 76.984 | 126 | 12 | 1 | 1 | 126 | 1 | 109 | 2.45e-65 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGAAATTTCACCTTCAT+TGG | 0.215806 | 4.3:-10164792 | MS.gene061786:CDS |
| ATGGCCATGATTAGAGAATT+TGG | 0.218589 | 4.3:-10164956 | MS.gene061786:CDS |
| CCTTCCTTCTCCATCGTTGA+TGG | 0.281713 | 4.3:+10164820 | None:intergenic |
| TATAAACTATCACAATTTAC+TGG | 0.291836 | 4.3:-10165151 | MS.gene061786:CDS |
| TCTGTCATCTTCCACAGTTT+TGG | 0.311646 | 4.3:+10164872 | None:intergenic |
| GCAGTGGGCTTTCACGAGTA+TGG | 0.337039 | 4.3:-10165053 | MS.gene061786:CDS |
| AATGACTTCCAGTCAGCATC+TGG | 0.351903 | 4.3:+10164983 | None:intergenic |
| AGTCAGCATCTGGGTTCTCT+TGG | 0.369174 | 4.3:+10164993 | None:intergenic |
| AGACGGAGATGGATGGAATA+TGG | 0.373466 | 4.3:-10165183 | None:intergenic |
| AGTCGCAAGGATAATAGAAT+TGG | 0.376598 | 4.3:-10164725 | MS.gene061786:CDS |
| TTGATGGTCTTCTCTAATAC+TGG | 0.390106 | 4.3:+10164836 | None:intergenic |
| GTCGCAAGGATAATAGAATT+GGG | 0.427469 | 4.3:-10164724 | MS.gene061786:CDS |
| AATTTCACCTTCATTGGAAA+AGG | 0.429481 | 4.3:-10164786 | MS.gene061786:CDS |
| GGAAGAGTATTGAACGAGAA+AGG | 0.472123 | 4.3:-10164701 | MS.gene061786:CDS |
| AAAGGCTGTTGATGCAGAAA+TGG | 0.484427 | 4.3:-10164768 | MS.gene061786:CDS |
| TGCTCCAAATTCTCTAATCA+TGG | 0.486981 | 4.3:+10164952 | None:intergenic |
| AAGAGAACCCAGATGCTGAC+TGG | 0.487075 | 4.3:-10164991 | MS.gene061786:CDS |
| TAGAGAATTTGGAGCACAGA+TGG | 0.487834 | 4.3:-10164945 | MS.gene061786:CDS |
| GAAGAGTATTGAACGAGAAA+GGG | 0.541967 | 4.3:-10164700 | MS.gene061786:CDS |
| ATCCTGCTGGATGGATCACA+AGG | 0.542589 | 4.3:-10165123 | MS.gene061786:CDS |
| GGGACAGATGAAGAAACAAA+AGG | 0.548268 | 4.3:-10164680 | MS.gene061786:CDS |
| GCACTCAACAGATAAACAAA+TGG | 0.550205 | 4.3:-10164921 | MS.gene061786:CDS |
| GCCCTTGTGATCCATCCAGC+AGG | 0.555500 | 4.3:+10165121 | None:intergenic |
| CTGGGACAGATCCTGCTGGA+TGG | 0.566751 | 4.3:-10165132 | MS.gene061786:CDS |
| CTCATGTGATAAGTTGCAGT+GGG | 0.571745 | 4.3:-10165068 | MS.gene061786:CDS |
| AAGACCATCAACGATGGAGA+AGG | 0.574882 | 4.3:-10164824 | MS.gene061786:CDS |
| TTATCGGAGACGGAGATGGA+TGG | 0.590795 | 4.3:-10165190 | None:intergenic |
| ATGACTTCCAGTCAGCATCT+GGG | 0.605808 | 4.3:+10164984 | None:intergenic |
| TCGCAAGGATAATAGAATTG+GGG | 0.613970 | 4.3:-10164723 | MS.gene061786:CDS |
| TTTACTGGGACAGATCCTGC+TGG | 0.616160 | 4.3:-10165136 | MS.gene061786:CDS |
| AGGATGAAGAAGCGTTGTTG+TGG | 0.617881 | 4.3:-10165030 | MS.gene061786:CDS |
| ATAAACTATCACAATTTACT+GGG | 0.624745 | 4.3:-10165150 | MS.gene061786:CDS |
| CGCAAGGATAATAGAATTGG+GGG | 0.628383 | 4.3:-10164722 | MS.gene061786:CDS |
| ACTCATGTGATAAGTTGCAG+TGG | 0.639256 | 4.3:-10165069 | MS.gene061786:CDS |
| GTGGGCTTTCACGAGTATGG+AGG | 0.656094 | 4.3:-10165050 | MS.gene061786:CDS |
| TGAAACAAAGAAGAGTCGCA+AGG | 0.658704 | 4.3:-10164738 | MS.gene061786:CDS |
| CCATCAACGATGGAGAAGGA+AGG | 0.677714 | 4.3:-10164820 | MS.gene061786:CDS |
| AAGTATCAGAACCAAAACTG+TGG | 0.682008 | 4.3:-10164883 | MS.gene061786:CDS |
| TTAGAGAAGACCATCAACGA+TGG | 0.682516 | 4.3:-10164830 | MS.gene061786:CDS |
| TCCTGCTGGATGGATCACAA+GGG | 0.711736 | 4.3:-10165122 | MS.gene061786:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAACTATCACAATTTACT+GGG | - | chr4.3:10164653-10164672 | MS.gene061786:CDS | 20.0% |
| !! | TATAAACTATCACAATTTAC+TGG | - | chr4.3:10164652-10164671 | MS.gene061786:CDS | 20.0% |
| AATTTCACCTTCATTGGAAA+AGG | - | chr4.3:10165017-10165036 | MS.gene061786:CDS | 30.0% | |
| AAGTATCAGAACCAAAACTG+TGG | - | chr4.3:10164920-10164939 | MS.gene061786:CDS | 35.0% | |
| AGTCGCAAGGATAATAGAAT+TGG | - | chr4.3:10165078-10165097 | MS.gene061786:CDS | 35.0% | |
| ATGGCCATGATTAGAGAATT+TGG | - | chr4.3:10164847-10164866 | MS.gene061786:CDS | 35.0% | |
| CAGTGAAATTTCACCTTCAT+TGG | - | chr4.3:10165011-10165030 | MS.gene061786:CDS | 35.0% | |
| GCACTCAACAGATAAACAAA+TGG | - | chr4.3:10164882-10164901 | MS.gene061786:CDS | 35.0% | |
| GTCGCAAGGATAATAGAATT+GGG | - | chr4.3:10165079-10165098 | MS.gene061786:CDS | 35.0% | |
| TCGCAAGGATAATAGAATTG+GGG | - | chr4.3:10165080-10165099 | MS.gene061786:CDS | 35.0% | |
| TGCTCCAAATTCTCTAATCA+TGG | + | chr4.3:10164854-10164873 | None:intergenic | 35.0% | |
| ! | TTGATGGTCTTCTCTAATAC+TGG | + | chr4.3:10164970-10164989 | None:intergenic | 35.0% |
| !! | GAAGAGTATTGAACGAGAAA+GGG | - | chr4.3:10165103-10165122 | MS.gene061786:CDS | 35.0% |
| !! | TGACTGGAAGTCATTTTCTA+TGG | - | chr4.3:10164828-10164847 | MS.gene061786:CDS | 35.0% |
| !!! | CGTTGTTGTGGTTTTATTCT+TGG | - | chr4.3:10164785-10164804 | MS.gene061786:CDS | 35.0% |
| AAAGGCTGTTGATGCAGAAA+TGG | - | chr4.3:10165035-10165054 | MS.gene061786:CDS | 40.0% | |
| ACTCATGTGATAAGTTGCAG+TGG | - | chr4.3:10164734-10164753 | MS.gene061786:CDS | 40.0% | |
| CGCAAGGATAATAGAATTGG+GGG | - | chr4.3:10165081-10165100 | MS.gene061786:CDS | 40.0% | |
| CTCATGTGATAAGTTGCAGT+GGG | - | chr4.3:10164735-10164754 | MS.gene061786:CDS | 40.0% | |
| GGGACAGATGAAGAAACAAA+AGG | - | chr4.3:10165123-10165142 | MS.gene061786:CDS | 40.0% | |
| TGAAACAAAGAAGAGTCGCA+AGG | - | chr4.3:10165065-10165084 | MS.gene061786:CDS | 40.0% | |
| TTAGAGAAGACCATCAACGA+TGG | - | chr4.3:10164973-10164992 | MS.gene061786:CDS | 40.0% | |
| ! | TAGAGAATTTGGAGCACAGA+TGG | - | chr4.3:10164858-10164877 | MS.gene061786:CDS | 40.0% |
| ! | TCAACAGCCTTTTCCAATGA+AGG | + | chr4.3:10165027-10165046 | None:intergenic | 40.0% |
| ! | TCTGTCATCTTCCACAGTTT+TGG | + | chr4.3:10164934-10164953 | None:intergenic | 40.0% |
| !! | GGAAGAGTATTGAACGAGAA+AGG | - | chr4.3:10165102-10165121 | MS.gene061786:CDS | 40.0% |
| AAGACCATCAACGATGGAGA+AGG | - | chr4.3:10164979-10164998 | MS.gene061786:CDS | 45.0% | |
| AATGACTTCCAGTCAGCATC+TGG | + | chr4.3:10164823-10164842 | None:intergenic | 45.0% | |
| ATGACTTCCAGTCAGCATCT+GGG | + | chr4.3:10164822-10164841 | None:intergenic | 45.0% | |
| !! | AGGATGAAGAAGCGTTGTTG+TGG | - | chr4.3:10164773-10164792 | MS.gene061786:CDS | 45.0% |
| AAGAGAACCCAGATGCTGAC+TGG | - | chr4.3:10164812-10164831 | MS.gene061786:CDS | 50.0% | |
| AGTCAGCATCTGGGTTCTCT+TGG | + | chr4.3:10164813-10164832 | None:intergenic | 50.0% | |
| ATCCTGCTGGATGGATCACA+AGG | - | chr4.3:10164680-10164699 | MS.gene061786:CDS | 50.0% | |
| CCATCAACGATGGAGAAGGA+AGG | - | chr4.3:10164983-10165002 | MS.gene061786:CDS | 50.0% | |
| CCTTCCTTCTCCATCGTTGA+TGG | + | chr4.3:10164986-10165005 | None:intergenic | 50.0% | |
| TCCTGCTGGATGGATCACAA+GGG | - | chr4.3:10164681-10164700 | MS.gene061786:CDS | 50.0% | |
| TTTACTGGGACAGATCCTGC+TGG | - | chr4.3:10164667-10164686 | MS.gene061786:CDS | 50.0% | |
| GTGGGCTTTCACGAGTATGG+AGG | - | chr4.3:10164753-10164772 | MS.gene061786:CDS | 55.0% | |
| ! | GCAGTGGGCTTTCACGAGTA+TGG | - | chr4.3:10164750-10164769 | MS.gene061786:CDS | 55.0% |
| CTGGGACAGATCCTGCTGGA+TGG | - | chr4.3:10164671-10164690 | MS.gene061786:CDS | 60.0% | |
| GCCCTTGTGATCCATCCAGC+AGG | + | chr4.3:10164685-10164704 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 10164628 | 10165197 | 10164628 | ID=MS.gene061786 |
| chr4.3 | mRNA | 10164628 | 10165197 | 10164628 | ID=MS.gene061786.t1;Parent=MS.gene061786 |
| chr4.3 | exon | 10164628 | 10165197 | 10164628 | ID=MS.gene061786.t1.exon1;Parent=MS.gene061786.t1 |
| chr4.3 | CDS | 10164628 | 10165197 | 10164628 | ID=cds.MS.gene061786.t1;Parent=MS.gene061786.t1 |
| Gene Sequence |
| Protein sequence |
