Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061883.t1 | XP_003625486.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061883.t1 | Q2HT99 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.0e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050584 | MS.gene061883 | 0.822672 | 2.09E-53 | -1.69E-46 |
| MS.gene059006 | MS.gene061883 | 0.801089 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061883.t1 | MTR_7g099670 | 98.077 | 104 | 2 | 0 | 1 | 104 | 1 | 104 | 3.16e-60 | 179 |
| MS.gene061883.t1 | MTR_1g069085 | 68.000 | 50 | 14 | 2 | 1 | 49 | 1 | 49 | 5.67e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGGTGGACTTGGTGGTTT+GGG | 0.209445 | 7.4:-10611350 | MS.gene061883:CDS |
| ACATTTGCTGGTGGTTATTC+AGG | 0.231052 | 7.4:-10611446 | MS.gene061883:CDS |
| GGAGGACTTGGTGGGGTTGG+TGG | 0.235350 | 7.4:-10611329 | MS.gene061883:CDS |
| CCTAGGTGGACTTGGTGGTT+TGG | 0.244833 | 7.4:-10611351 | MS.gene061883:CDS |
| CTTGGTGGTTTGGGAGGACT+TGG | 0.272357 | 7.4:-10611341 | MS.gene061883:CDS |
| GGACTTGGTGGGGTTGGTGG+TGG | 0.293034 | 7.4:-10611326 | MS.gene061883:CDS |
| GGTGGAGCTGGTGGTGGTGT+TGG | 0.293326 | 7.4:-10611308 | MS.gene061883:CDS |
| GGTGGAATCGGTGGTGATTT+AGG | 0.312961 | 7.4:-10611386 | MS.gene061883:CDS |
| GGTGGTTTGGGAGGACTTGG+TGG | 0.314590 | 7.4:-10611338 | MS.gene061883:CDS |
| TTGGGAGGACTTGGTGGGGT+TGG | 0.314725 | 7.4:-10611332 | MS.gene061883:CDS |
| GGTGGGGTTGGTGGTGGAGC+TGG | 0.316689 | 7.4:-10611320 | MS.gene061883:CDS |
| GTTGGTGGTGGAGCTGGTGG+TGG | 0.321018 | 7.4:-10611314 | MS.gene061883:CDS |
| GGAAACAATGGACTTCCTTT+TGG | 0.322482 | 7.4:-10611419 | MS.gene061883:CDS |
| TTAGGTGGTGGTGGTGGCCT+AGG | 0.323708 | 7.4:-10611368 | MS.gene061883:CDS |
| GGTGGTGGCCTAGGTGGACT+TGG | 0.331166 | 7.4:-10611359 | MS.gene061883:CDS |
| GGTGGTGTTGGAGGTGGTGT+TGG | 0.331290 | 7.4:-10611296 | MS.gene061883:CDS |
| GGGGTTGGTGGTGGAGCTGG+TGG | 0.333006 | 7.4:-10611317 | MS.gene061883:CDS |
| GCTGGTGGTTATTCAGGAAT+AGG | 0.341133 | 7.4:-10611440 | MS.gene061883:CDS |
| GTTGGAGGTGGTGTTGGAAC+TGG | 0.344227 | 7.4:-10611290 | MS.gene061883:CDS |
| CAAATGTCATGAAGTTCTTC+TGG | 0.354882 | 7.4:+10611462 | None:intergenic |
| GCTGGTGGTGGTGTTGGAGG+TGG | 0.373109 | 7.4:-10611302 | MS.gene061883:CDS |
| AAGAACTTCATGACATTTGC+TGG | 0.375624 | 7.4:-10611458 | MS.gene061883:CDS |
| GGAGCTGGTGGTGGTGTTGG+AGG | 0.379910 | 7.4:-10611305 | MS.gene061883:CDS |
| GGTGGTGTTGGAACTGGTGT+TGG | 0.385912 | 7.4:-10611284 | MS.gene061883:CDS |
| GGTGGAATTGGAGGTGGAAT+CGG | 0.386910 | 7.4:-10611398 | MS.gene061883:CDS |
| GTGGTTTGGGAGGACTTGGT+GGG | 0.431797 | 7.4:-10611337 | MS.gene061883:CDS |
| TGGTTTGGGAGGACTTGGTG+GGG | 0.448017 | 7.4:-10611336 | MS.gene061883:CDS |
| GGTGTTGGAACTGGTGTTGG+TGG | 0.458137 | 7.4:-10611281 | MS.gene061883:CDS |
| AAAGTGGTGTATGTTGTTGT+TGG | 0.463389 | 7.4:-10611549 | MS.gene061883:CDS |
| GGTGGCCTAGGTGGACTTGG+TGG | 0.480801 | 7.4:-10611356 | MS.gene061883:CDS |
| GGTGGACTTGGTGGTTTGGG+AGG | 0.508576 | 7.4:-10611347 | MS.gene061883:CDS |
| GTTGGTTCTTGCTCTTGTTG+TGG | 0.516173 | 7.4:-10611531 | MS.gene061883:CDS |
| GGTGGTGGTGGTGGCCTAGG+TGG | 0.516203 | 7.4:-10611365 | MS.gene061883:CDS |
| ACCAACTGATGCTGGTTTGA+AGG | 0.518003 | 7.4:-10611486 | MS.gene061883:CDS |
| GGTGATTTAGGTGGTGGTGG+TGG | 0.527759 | 7.4:-10611374 | MS.gene061883:CDS |
| ATCGGTGGTGATTTAGGTGG+TGG | 0.527781 | 7.4:-10611380 | MS.gene061883:CDS |
| CCACCTCCAATTCCACCAAA+AGG | 0.542402 | 7.4:+10611404 | None:intergenic |
| GGAATTGGAGGTGGAATCGG+TGG | 0.552447 | 7.4:-10611395 | MS.gene061883:CDS |
| GGTGGTGATTTAGGTGGTGG+TGG | 0.559090 | 7.4:-10611377 | MS.gene061883:CDS |
| GAATACTGATCTTAAGGGAA+AGG | 0.564229 | 7.4:+10611251 | None:intergenic |
| AGAAATGTACCAACTGATGC+TGG | 0.575990 | 7.4:-10611494 | MS.gene061883:CDS |
| TATTCAGGAATAGGAAACAA+TGG | 0.605549 | 7.4:-10611431 | MS.gene061883:CDS |
| GGAATCGGTGGTGATTTAGG+TGG | 0.612801 | 7.4:-10611383 | MS.gene061883:CDS |
| GAGTGAGAGATATGGCAAAG+TGG | 0.614546 | 7.4:-10611565 | None:intergenic |
| AACTTCATGACATTTGCTGG+TGG | 0.621619 | 7.4:-10611455 | MS.gene061883:CDS |
| TCCTTCAAACCAGCATCAGT+TGG | 0.622405 | 7.4:+10611485 | None:intergenic |
| CCAAACCACCAAGTCCACCT+AGG | 0.637542 | 7.4:+10611351 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCAGGAATAGGAAACAA+TGG | - | chr7.4:10611385-10611404 | MS.gene061883:CDS | 30.0% | |
| AAAGTGGTGTATGTTGTTGT+TGG | - | chr7.4:10611267-10611286 | MS.gene061883:CDS | 35.0% | |
| AAGAACTTCATGACATTTGC+TGG | - | chr7.4:10611358-10611377 | MS.gene061883:CDS | 35.0% | |
| CAAATGTCATGAAGTTCTTC+TGG | + | chr7.4:10611357-10611376 | None:intergenic | 35.0% | |
| AGAAATGTACCAACTGATGC+TGG | - | chr7.4:10611322-10611341 | MS.gene061883:CDS | 40.0% | |
| ! | AACTTCATGACATTTGCTGG+TGG | - | chr7.4:10611361-10611380 | MS.gene061883:CDS | 40.0% |
| ! | ACATTTGCTGGTGGTTATTC+AGG | - | chr7.4:10611370-10611389 | MS.gene061883:CDS | 40.0% |
| !! | AACAATGGACTTCCTTTTGG+TGG | - | chr7.4:10611400-10611419 | MS.gene061883:CDS | 40.0% |
| !! | GGAAACAATGGACTTCCTTT+TGG | - | chr7.4:10611397-10611416 | MS.gene061883:CDS | 40.0% |
| GCTGGTGGTTATTCAGGAAT+AGG | - | chr7.4:10611376-10611395 | MS.gene061883:CDS | 45.0% | |
| TCCTTCAAACCAGCATCAGT+TGG | + | chr7.4:10611334-10611353 | None:intergenic | 45.0% | |
| ! | CTTCCTTTTGGTGGAATTGG+AGG | - | chr7.4:10611409-10611428 | MS.gene061883:CDS | 45.0% |
| ! | GGACTTCCTTTTGGTGGAAT+TGG | - | chr7.4:10611406-10611425 | MS.gene061883:CDS | 45.0% |
| ! | GTTGGTTCTTGCTCTTGTTG+TGG | - | chr7.4:10611285-10611304 | MS.gene061883:CDS | 45.0% |
| !! | ACCAACTGATGCTGGTTTGA+AGG | - | chr7.4:10611330-10611349 | MS.gene061883:CDS | 45.0% |
| ATCGGTGGTGATTTAGGTGG+TGG | - | chr7.4:10611436-10611455 | MS.gene061883:CDS | 50.0% | |
| CCACCTCCAATTCCACCAAA+AGG | + | chr7.4:10611415-10611434 | None:intergenic | 50.0% | |
| GGAATCGGTGGTGATTTAGG+TGG | - | chr7.4:10611433-10611452 | MS.gene061883:CDS | 50.0% | |
| GGTGGAATCGGTGGTGATTT+AGG | - | chr7.4:10611430-10611449 | MS.gene061883:CDS | 50.0% | |
| GGTGGAATTGGAGGTGGAAT+CGG | - | chr7.4:10611418-10611437 | MS.gene061883:CDS | 50.0% | |
| ! | CCTTTTGGTGGAATTGGAGG+TGG | - | chr7.4:10611412-10611431 | MS.gene061883:CDS | 50.0% |
| !! | CTAGGTGGACTTGGTGGTTT+GGG | - | chr7.4:10611466-10611485 | MS.gene061883:CDS | 50.0% |
| CCAAACCACCAAGTCCACCT+AGG | + | chr7.4:10611468-10611487 | None:intergenic | 55.0% | |
| GGAATTGGAGGTGGAATCGG+TGG | - | chr7.4:10611421-10611440 | MS.gene061883:CDS | 55.0% | |
| ! | CTTGGTGGTTTGGGAGGACT+TGG | - | chr7.4:10611475-10611494 | MS.gene061883:CDS | 55.0% |
| ! | GGTGGTGATTTAGGTGGTGG+TGG | - | chr7.4:10611439-10611458 | MS.gene061883:CDS | 55.0% |
| ! | GGTGGTGTTGGAACTGGTGT+TGG | - | chr7.4:10611532-10611551 | MS.gene061883:CDS | 55.0% |
| ! | GGTGTTGGAACTGGTGTTGG+TGG | - | chr7.4:10611535-10611554 | MS.gene061883:CDS | 55.0% |
| ! | GTGGTTTGGGAGGACTTGGT+GGG | - | chr7.4:10611479-10611498 | MS.gene061883:CDS | 55.0% |
| ! | GTTGGAGGTGGTGTTGGAAC+TGG | - | chr7.4:10611526-10611545 | MS.gene061883:CDS | 55.0% |
| !! | CCTAGGTGGACTTGGTGGTT+TGG | - | chr7.4:10611465-10611484 | MS.gene061883:CDS | 55.0% |
| !! | GGTGATTTAGGTGGTGGTGG+TGG | - | chr7.4:10611442-10611461 | MS.gene061883:CDS | 55.0% |
| !! | TGGTTTGGGAGGACTTGGTG+GGG | - | chr7.4:10611480-10611499 | MS.gene061883:CDS | 55.0% |
| ! | GGTGGTGTTGGAGGTGGTGT+TGG | - | chr7.4:10611520-10611539 | MS.gene061883:CDS | 60.0% |
| ! | GGTGGTTTGGGAGGACTTGG+TGG | - | chr7.4:10611478-10611497 | MS.gene061883:CDS | 60.0% |
| !! | GGTGGACTTGGTGGTTTGGG+AGG | - | chr7.4:10611469-10611488 | MS.gene061883:CDS | 60.0% |
| !! | TTAGGTGGTGGTGGTGGCCT+AGG | - | chr7.4:10611448-10611467 | MS.gene061883:CDS | 60.0% |
| !! | TTGGGAGGACTTGGTGGGGT+TGG | - | chr7.4:10611484-10611503 | MS.gene061883:CDS | 60.0% |
| ! | GGTGGCCTAGGTGGACTTGG+TGG | - | chr7.4:10611460-10611479 | MS.gene061883:CDS | 65.0% |
| ! | GGTGGTGGCCTAGGTGGACT+TGG | - | chr7.4:10611457-10611476 | MS.gene061883:CDS | 65.0% |
| !! | GCTGGTGGTGGTGTTGGAGG+TGG | - | chr7.4:10611514-10611533 | MS.gene061883:CDS | 65.0% |
| !! | GGACTTGGTGGGGTTGGTGG+TGG | - | chr7.4:10611490-10611509 | MS.gene061883:CDS | 65.0% |
| !! | GGAGCTGGTGGTGGTGTTGG+AGG | - | chr7.4:10611511-10611530 | MS.gene061883:CDS | 65.0% |
| !! | GGAGGACTTGGTGGGGTTGG+TGG | - | chr7.4:10611487-10611506 | MS.gene061883:CDS | 65.0% |
| !! | GGTGGAGCTGGTGGTGGTGT+TGG | - | chr7.4:10611508-10611527 | MS.gene061883:CDS | 65.0% |
| !! | GTTGGTGGTGGAGCTGGTGG+TGG | - | chr7.4:10611502-10611521 | MS.gene061883:CDS | 65.0% |
| !! | GGGGTTGGTGGTGGAGCTGG+TGG | - | chr7.4:10611499-10611518 | MS.gene061883:CDS | 70.0% |
| !! | GGTGGGGTTGGTGGTGGAGC+TGG | - | chr7.4:10611496-10611515 | MS.gene061883:CDS | 70.0% |
| !! | GGTGGTGGTGGTGGCCTAGG+TGG | - | chr7.4:10611451-10611470 | MS.gene061883:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10611262 | 10611576 | 10611262 | ID=MS.gene061883 |
| chr7.4 | mRNA | 10611262 | 10611576 | 10611262 | ID=MS.gene061883.t1;Parent=MS.gene061883 |
| chr7.4 | exon | 10611262 | 10611576 | 10611262 | ID=MS.gene061883.t1.exon1;Parent=MS.gene061883.t1 |
| chr7.4 | CDS | 10611262 | 10611576 | 10611262 | ID=cds.MS.gene061883.t1;Parent=MS.gene061883.t1 |
| Gene Sequence |
| Protein sequence |
