AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059006


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059006.t1 MTR_1g069085 94.643 56 3 0 1 56 1 56 3.45e-26 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGATGCTGGTGGTGGACT+TGG 0.309365 1.2:+51819774 MS.gene059006:CDS
CTCCCCGGACTTGGCGGTGC+TGG 0.314858 1.2:+51819852 MS.gene059006:CDS
CTCCCCGGACTTGGCGGTGC+TGG 0.314858 1.2:+51819879 MS.gene059006:CDS
GGTAACAATGGAATGCCTTT+TGG 0.333736 1.2:+51819729 MS.gene059006:CDS
TTTGGTGGTGCTGGTGATGC+TGG 0.337285 1.2:+51819762 MS.gene059006:CDS
CTTGGCGGTGCTGGTGGACT+CGG 0.338493 1.2:+51819888 MS.gene059006:CDS
TCCACCAGCACCGCCAAGTC+CGG 0.356337 1.2:-51819856 None:intergenic
TCCACCAGCACCGCCAAGTC+CGG 0.368793 1.2:-51819883 None:intergenic
GGTGTAGGCTTTGGTGGTGC+TGG 0.371956 1.2:+51819753 MS.gene059006:CDS
GGTGGTGGACTCCCCGGACT+TGG 0.373489 1.2:+51819843 MS.gene059006:CDS
GCTGGTGGACTCGGAGGACT+TGG 0.376726 1.2:+51819897 MS.gene059006:CDS
GCTGGTGGACTCCCCGGACT+TGG 0.385564 1.2:+51819870 MS.gene059006:CDS
GGTGGCGGTGCTGGTGGACT+CGG 0.386691 1.2:+51819804 MS.gene059006:CDS
CTCGGAGGACTTGGTGGTAC+TGG 0.399755 1.2:+51819906 MS.gene059006:CDS
GGTGCTGGTGATGCTGGTGG+TGG 0.408529 1.2:+51819768 MS.gene059006:CDS
GCTGGTGGACTCGGAGGAAT+CGG 0.409280 1.2:+51819813 MS.gene059006:CDS
GGAATCGGTGGTGGTGGTGG+TGG 0.410946 1.2:+51819828 MS.gene059006:CDS
GGTGGTGGACTTGGAGGACT+AGG 0.420340 1.2:+51819783 MS.gene059006:CDS
CCACCAGCACCGCCAAGTCC+GGG 0.427720 1.2:-51819855 None:intergenic
CCACCAGCACCGCCAAGTCC+GGG 0.427720 1.2:-51819882 None:intergenic
GGAGGAATCGGTGGTGGTGG+TGG 0.434584 1.2:+51819825 MS.gene059006:CDS
CTCGGAGGAATCGGTGGTGG+TGG 0.446365 1.2:+51819822 MS.gene059006:CDS
GGCGGTGCTGGTGGACTCCC+CGG 0.447008 1.2:+51819864 MS.gene059006:CDS
GGTGGTGGTGGTGGACTCCC+CGG 0.450226 1.2:+51819837 MS.gene059006:CDS
GGAGGACTAGGTGGCGGTGC+TGG 0.453242 1.2:+51819795 MS.gene059006:CDS
CCCGGACTTGGCGGTGCTGG+TGG 0.458285 1.2:+51819855 MS.gene059006:CDS
CCCGGACTTGGCGGTGCTGG+TGG 0.458285 1.2:+51819882 MS.gene059006:CDS
TTTGGAGGTGTAGGCTTTGG+TGG 0.458483 1.2:+51819747 MS.gene059006:CDS
AATGTCTAAGTGGTGCATTT+TGG 0.458799 1.2:+51819593 None:intergenic
GATGCTGGTGGTGGACTTGG+AGG 0.463937 1.2:+51819777 MS.gene059006:CDS
GGACTCGGAGGAATCGGTGG+TGG 0.495212 1.2:+51819819 MS.gene059006:CDS
AAGAATTACATGACTGGAGC+TGG 0.500445 1.2:+51819690 MS.gene059006:CDS
GGACTAGGTGGCGGTGCTGG+TGG 0.509217 1.2:+51819798 MS.gene059006:CDS
GGACTTGGTGGTACTGGTGG+TGG 0.520354 1.2:+51819912 MS.gene059006:CDS
GGTGGACTCCCCGGACTTGG+CGG 0.520746 1.2:+51819873 MS.gene059006:CDS
TGCTGGAGAAGATGGTTTGA+AGG 0.527688 1.2:+51819662 MS.gene059006:CDS
TTCAAACCATCTTCTCCAGC+AGG 0.528729 1.2:-51819660 None:intergenic
TAACTTGACACTCAAGGTTG+AGG 0.530118 1.2:-51819942 None:intergenic
GGTGGACTCCCCGGACTTGG+CGG 0.531283 1.2:+51819846 MS.gene059006:CDS
CCAAAGCCTACACCTCCAAA+AGG 0.544740 1.2:-51819744 None:intergenic
GATCAGAAGAATTACATGAC+TGG 0.553149 1.2:+51819684 MS.gene059006:CDS
GGTGGTGCTGGTGATGCTGG+TGG 0.553318 1.2:+51819765 MS.gene059006:CDS
AATGCAAGAAATGTGCCTGC+TGG 0.560154 1.2:+51819645 MS.gene059006:CDS
GGTGGACTCGGAGGACTTGG+TGG 0.562731 1.2:+51819900 MS.gene059006:CDS
AATGTGCCTGCTGGAGAAGA+TGG 0.564431 1.2:+51819654 MS.gene059006:CDS
GGTGGACTCGGAGGAATCGG+TGG 0.565750 1.2:+51819816 MS.gene059006:CDS
CACCAGCACCGCCAAGTCCG+GGG 0.580869 1.2:-51819854 None:intergenic
CACCAGCACCGCCAAGTCCG+GGG 0.580869 1.2:-51819881 None:intergenic
GGCGGTGCTGGTGGACTCGG+AGG 0.589973 1.2:+51819807 MS.gene059006:CDS
GGAGGACTTGGTGGTACTGG+TGG 0.611733 1.2:+51819909 MS.gene059006:CDS
GGCGGTGCTGGTGGACTCGG+AGG 0.621136 1.2:+51819891 MS.gene059006:CDS
GGTGGACTTGGAGGACTAGG+TGG 0.622805 1.2:+51819786 MS.gene059006:CDS
GGACTTGGAGGACTAGGTGG+CGG 0.646664 1.2:+51819789 MS.gene059006:CDS
TGAAGAGAGAAATGTCTAAG+TGG 0.655500 1.2:+51819583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GATCAGAAGAATTACATGAC+TGG + chr1.2:51819684-51819703 MS.gene059006:CDS 35.0%
!!! TTTTCAGGCATTGGTAACAA+TGG + chr1.2:51819717-51819736 MS.gene059006:CDS 35.0%
AAGAATTACATGACTGGAGC+TGG + chr1.2:51819690-51819709 MS.gene059006:CDS 40.0%
! AACAATGGAATGCCTTTTGG+AGG + chr1.2:51819732-51819751 MS.gene059006:CDS 40.0%
! GGTAACAATGGAATGCCTTT+TGG + chr1.2:51819729-51819748 MS.gene059006:CDS 40.0%
!!! GTGGTGCATTTTGGTTGTTT+TGG + chr1.2:51819602-51819621 MS.gene059006:CDS 40.0%
AATGCAAGAAATGTGCCTGC+TGG + chr1.2:51819645-51819664 MS.gene059006:CDS 45.0%
TGCTGGAGAAGATGGTTTGA+AGG + chr1.2:51819662-51819681 MS.gene059006:CDS 45.0%
TTCAAACCATCTTCTCCAGC+AGG - chr1.2:51819663-51819682 None:intergenic 45.0%
!!! ACTGGAGCTGGTGTTTTTTC+AGG + chr1.2:51819702-51819721 MS.gene059006:CDS 45.0%
!!! GCTGGTGTTTTTTCAGGCAT+TGG + chr1.2:51819708-51819727 MS.gene059006:CDS 45.0%
AATGTGCCTGCTGGAGAAGA+TGG + chr1.2:51819654-51819673 MS.gene059006:CDS 50.0%
CCAAAGCCTACACCTCCAAA+AGG - chr1.2:51819747-51819766 None:intergenic 50.0%
! TTTGGAGGTGTAGGCTTTGG+TGG + chr1.2:51819747-51819766 MS.gene059006:CDS 50.0%
!! CCTTTTGGAGGTGTAGGCTT+TGG + chr1.2:51819744-51819763 MS.gene059006:CDS 50.0%
!! GGAATGCCTTTTGGAGGTGT+AGG + chr1.2:51819738-51819757 MS.gene059006:CDS 50.0%
!!! GGTTGTTTTGGCTCTTGCTG+TGG + chr1.2:51819614-51819633 MS.gene059006:CDS 50.0%
!! TTTGGTGGTGCTGGTGATGC+TGG + chr1.2:51819762-51819781 MS.gene059006:CDS 55.0%
GGACTTGGAGGACTAGGTGG+CGG + chr1.2:51819789-51819808 MS.gene059006:CDS 60.0%
! GGTGGACTTGGAGGACTAGG+TGG + chr1.2:51819786-51819805 MS.gene059006:CDS 60.0%
!! CTCGGAGGACTTGGTGGTAC+TGG + chr1.2:51819906-51819925 MS.gene059006:CDS 60.0%
!! GATGCTGGTGGTGGACTTGG+AGG + chr1.2:51819777-51819796 MS.gene059006:CDS 60.0%
!! GCTGGTGGACTCGGAGGAAT+CGG + chr1.2:51819813-51819832 MS.gene059006:CDS 60.0%
!! GGACTTGGTGGTACTGGTGG+TGG + chr1.2:51819912-51819931 MS.gene059006:CDS 60.0%
!! GGAGGACTTGGTGGTACTGG+TGG + chr1.2:51819909-51819928 MS.gene059006:CDS 60.0%
!! GGTGATGCTGGTGGTGGACT+TGG + chr1.2:51819774-51819793 MS.gene059006:CDS 60.0%
!! GGTGGTGGACTTGGAGGACT+AGG + chr1.2:51819783-51819802 MS.gene059006:CDS 60.0%
!! GGTGTAGGCTTTGGTGGTGC+TGG + chr1.2:51819753-51819772 MS.gene059006:CDS 60.0%
GGACTCGGAGGAATCGGTGG+TGG + chr1.2:51819819-51819838 MS.gene059006:CDS 65.0%
TCCACCAGCACCGCCAAGTC+CGG - chr1.2:51819859-51819878 None:intergenic 65.0%
! CTCGGAGGAATCGGTGGTGG+TGG + chr1.2:51819822-51819841 MS.gene059006:CDS 65.0%
! GGTGGACTCGGAGGAATCGG+TGG + chr1.2:51819816-51819835 MS.gene059006:CDS 65.0%
! GGTGGACTCGGAGGACTTGG+TGG + chr1.2:51819900-51819919 MS.gene059006:CDS 65.0%
!! CTTGGCGGTGCTGGTGGACT+CGG + chr1.2:51819861-51819880 MS.gene059006:CDS 65.0%
!! GCTGGTGGACTCGGAGGACT+TGG + chr1.2:51819897-51819916 MS.gene059006:CDS 65.0%
!! GGAATCGGTGGTGGTGGTGG+TGG + chr1.2:51819828-51819847 MS.gene059006:CDS 65.0%
!! GGAGGAATCGGTGGTGGTGG+TGG + chr1.2:51819825-51819844 MS.gene059006:CDS 65.0%
!! GGTGCTGGTGATGCTGGTGG+TGG + chr1.2:51819768-51819787 MS.gene059006:CDS 65.0%
!! GGTGGTGCTGGTGATGCTGG+TGG + chr1.2:51819765-51819784 MS.gene059006:CDS 65.0%
CACCAGCACCGCCAAGTCCG+GGG - chr1.2:51819857-51819876 None:intergenic 70.0%
CCACCAGCACCGCCAAGTCC+GGG - chr1.2:51819858-51819877 None:intergenic 70.0%
! GGACTAGGTGGCGGTGCTGG+TGG + chr1.2:51819798-51819817 MS.gene059006:CDS 70.0%
! GGAGGACTAGGTGGCGGTGC+TGG + chr1.2:51819795-51819814 MS.gene059006:CDS 70.0%
! GGTGGACTCCCCGGACTTGG+CGG + chr1.2:51819846-51819865 MS.gene059006:CDS 70.0%
!! GCTGGTGGACTCCCCGGACT+TGG + chr1.2:51819870-51819889 MS.gene059006:CDS 70.0%
!! GGTGGCGGTGCTGGTGGACT+CGG + chr1.2:51819804-51819823 MS.gene059006:CDS 70.0%
!! GGTGGTGGACTCCCCGGACT+TGG + chr1.2:51819843-51819862 MS.gene059006:CDS 70.0%
!! GGTGGTGGTGGTGGACTCCC+CGG + chr1.2:51819837-51819856 MS.gene059006:CDS 70.0%
CTCCCCGGACTTGGCGGTGC+TGG + chr1.2:51819852-51819871 MS.gene059006:CDS 75.0%
! CCCGGACTTGGCGGTGCTGG+TGG + chr1.2:51819855-51819874 MS.gene059006:CDS 75.0%
!! GGCGGTGCTGGTGGACTCCC+CGG + chr1.2:51819864-51819883 MS.gene059006:CDS 75.0%
!! GGCGGTGCTGGTGGACTCGG+AGG + chr1.2:51819807-51819826 MS.gene059006:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 51819594 51819953 51819594 ID=MS.gene059006
chr1.2 mRNA 51819594 51819953 51819594 ID=MS.gene059006.t1;Parent=MS.gene059006
chr1.2 exon 51819594 51819953 51819594 ID=MS.gene059006.t1.exon1;Parent=MS.gene059006.t1
chr1.2 CDS 51819594 51819953 51819594 ID=cds.MS.gene059006.t1;Parent=MS.gene059006.t1
Gene Sequence

>MS.gene059006

ATGTCTAAGTGGTGCATTTTGGTTGTTTTGGCTCTTGCTGTGGTTGCAACAAATGCAAGAAATGTGCCTGCTGGAGAAGATGGTTTGAAGGATCAGAAGAATTACATGACTGGAGCTGGTGTTTTTTCAGGCATTGGTAACAATGGAATGCCTTTTGGAGGTGTAGGCTTTGGTGGTGCTGGTGATGCTGGTGGTGGACTTGGAGGACTAGGTGGCGGTGCTGGTGGACTCGGAGGAATCGGTGGTGGTGGTGGTGGACTCCCCGGACTTGGCGGTGCTGGTGGACTCCCCGGACTTGGCGGTGCTGGTGGACTCGGAGGACTTGGTGGTACTGGTGGTGGTGTCTTTCCTCAACCTTGA

Protein sequence

>MS.gene059006.t1

MSKWCILVVLALAVVATNARNVPAGEDGLKDQKNYMTGAGVFSGIGNNGMPFGGVGFGGAGDAGGGLGGLGGGAGGLGGIGGGGGGLPGLGGAGGLPGLGGAGGLGGLGGTGGGVFPQP