Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061926.t1 | XP_004493910.1 | 90.9 | 405 | 35 | 2 | 1 | 403 | 36 | 440 | 6.10E-186 | 660.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061926.t1 | F4JY11 | 72.2 | 403 | 112 | 0 | 1 | 403 | 38 | 440 | 2.3e-162 | 573.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061926.t1 | A0A1S2XSL9 | 90.9 | 405 | 35 | 2 | 1 | 403 | 36 | 440 | 4.4e-186 | 660.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene25783 | MS.gene061926 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061926.t1 | MTR_7g100360 | 98.280 | 407 | 3 | 1 | 1 | 403 | 38 | 444 | 0.0 | 813 |
| MS.gene061926.t1 | MTR_1g067370 | 85.360 | 403 | 56 | 2 | 1 | 403 | 40 | 439 | 0.0 | 642 |
| MS.gene061926.t1 | MTR_3g080830 | 52.956 | 406 | 182 | 5 | 1 | 403 | 38 | 437 | 1.60e-146 | 423 |
| MS.gene061926.t1 | MTR_1g025450 | 59.333 | 150 | 59 | 1 | 1 | 150 | 315 | 462 | 5.39e-36 | 139 |
| MS.gene061926.t1 | MTR_1g061930 | 35.537 | 121 | 75 | 3 | 285 | 402 | 1 | 121 | 5.84e-19 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061926.t1 | AT5G18520 | 80.397 | 403 | 79 | 0 | 1 | 403 | 38 | 440 | 0.0 | 672 |
| MS.gene061926.t1 | AT3G09570 | 77.419 | 403 | 91 | 0 | 1 | 403 | 37 | 439 | 0.0 | 631 |
| MS.gene061926.t1 | AT5G02630 | 64.706 | 391 | 131 | 3 | 1 | 389 | 36 | 421 | 0.0 | 512 |
| MS.gene061926.t1 | AT5G42090 | 52.346 | 405 | 189 | 3 | 1 | 403 | 37 | 439 | 1.02e-149 | 431 |
Find 0 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTATATTGTTGTTATT+GGG | - | 51431:33580-33599 | MS.gene061926:CDS | 15.0% |
| !!! | ACTTAACTTTCTTTGTATTT+TGG | - | 51431:33058-33077 | MS.gene061926:CDS | 20.0% |
| !!! | CAGTTTTATATTGTTGTTAT+TGG | - | 51431:33579-33598 | MS.gene061926:CDS | 20.0% |
| !!! | TTTACTGTTATTGTTTTGAT+CGG | - | 51431:33270-33289 | MS.gene061926:CDS | 20.0% |
| AGAGGATAAGCATTATGTTA+AGG | - | 51431:33179-33198 | MS.gene061926:CDS | 30.0% | |
| ATCTAATAGACCAAACGATA+GGG | + | 51431:33491-33510 | None:intergenic | 30.0% | |
| ATGATCCTCTTCGAAAAATT+CGG | - | 51431:32613-32632 | MS.gene061926:CDS | 30.0% | |
| CAAAGAAAGTTAAGTAAGCA+AGG | + | 51431:33053-33072 | None:intergenic | 30.0% | |
| GTAACCCAATCTTTGATAAA+AGG | + | 51431:33414-33433 | None:intergenic | 30.0% | |
| TGAATCGAGATTGAAAATCT+CGG | + | 51431:32965-32984 | None:intergenic | 30.0% | |
| TTATCAAAGATTGGGTTACT+TGG | - | 51431:33415-33434 | MS.gene061926:CDS | 30.0% | |
| TTGGTCTATTAGATCACTTA+GGG | - | 51431:33497-33516 | MS.gene061926:CDS | 30.0% | |
| TTTGGTCTATTAGATCACTT+AGG | - | 51431:33496-33515 | MS.gene061926:CDS | 30.0% | |
| ! | ATTTTCAATCTCGATTCAGA+CGG | - | 51431:32967-32986 | MS.gene061926:CDS | 30.0% |
| ! | CTATTATTTTCCCTATCGTT+TGG | - | 51431:33478-33497 | MS.gene061926:CDS | 30.0% |
| ! | TGCTGCTTATAAGTATCAAT+GGG | - | 51431:33647-33666 | MS.gene061926:CDS | 30.0% |
| ! | TTGCTGCTTATAAGTATCAA+TGG | - | 51431:33646-33665 | MS.gene061926:CDS | 30.0% |
| ! | TTTTCCCTATCTTGCAAAAA+CGG | + | 51431:33318-33337 | None:intergenic | 30.0% |
| AAATTAGCCAAAACTTGCAG+AGG | + | 51431:33363-33382 | None:intergenic | 35.0% | |
| AAGAAGAGACTGTATTCGTT+AGG | + | 51431:32898-32917 | None:intergenic | 35.0% | |
| AAGAAGAGGAAAAAGAGAGA+AGG | + | 51431:33030-33049 | None:intergenic | 35.0% | |
| AAGCATTATGTTAAGGTCAC+TGG | - | 51431:33186-33205 | MS.gene061926:CDS | 35.0% | |
| AGAAGAGACTGTATTCGTTA+GGG | + | 51431:32897-32916 | None:intergenic | 35.0% | |
| AGAAGAGGAAAAAGAGAGAA+GGG | + | 51431:33029-33048 | None:intergenic | 35.0% | |
| GATCTAATAGACCAAACGAT+AGG | + | 51431:33492-33511 | None:intergenic | 35.0% | |
| GTTCTATATGTTTAGACCTG+TGG | - | 51431:33719-33738 | MS.gene061926:CDS | 35.0% | |
| TGAGTACTTTGTTCTTGATG+AGG | - | 51431:33749-33768 | MS.gene061926:CDS | 35.0% | |
| TTAAGTAAGCAAGGAAGAAG+AGG | + | 51431:33044-33063 | None:intergenic | 35.0% | |
| ! | AATTTAGCTTCCGTTGTGAT+TGG | - | 51431:33378-33397 | MS.gene061926:CDS | 35.0% |
| ! | GGGTCCTTTTATCAAAGATT+GGG | - | 51431:33407-33426 | MS.gene061926:CDS | 35.0% |
| !! | AAGTACTCATTTTTCTCCAC+AGG | + | 51431:33738-33757 | None:intergenic | 35.0% |
| !! | GAAACCGTTTTTGCAAGATA+GGG | - | 51431:33311-33330 | MS.gene061926:CDS | 35.0% |
| !! | TGAAACCGAATTTTTCGAAG+AGG | + | 51431:32621-32640 | None:intergenic | 35.0% |
| !! | TGAAACCGTTTTTGCAAGAT+AGG | - | 51431:33310-33329 | MS.gene061926:CDS | 35.0% |
| !!! | GTTATTGTTTTGATCGGTAC+TGG | - | 51431:33276-33295 | MS.gene061926:CDS | 35.0% |
| AAACAGATGGAAAAGCTTCG+AGG | - | 51431:33529-33548 | MS.gene061926:CDS | 40.0% | |
| AAATTCGGTTTCACTCCCAA+AGG | - | 51431:32628-32647 | MS.gene061926:CDS | 40.0% | |
| AACAATACATCCCATCCATG+AGG | + | 51431:33216-33235 | None:intergenic | 40.0% | |
| AACATGGAAACAGAGGTTTC+AGG | + | 51431:32934-32953 | None:intergenic | 40.0% | |
| AGATTGGGTTACTTGGAATC+AGG | - | 51431:33422-33441 | MS.gene061926:CDS | 40.0% | |
| AGGGAAACATCGAAAACAGA+TGG | - | 51431:33516-33535 | MS.gene061926:CDS | 40.0% | |
| CAGCCATAAGAAGATGAATG+CGG | + | 51431:33119-33138 | None:intergenic | 40.0% | |
| GAAGAGACTGTATTCGTTAG+GGG | + | 51431:32896-32915 | None:intergenic | 40.0% | |
| GCGGTGAATAGAACGTTTAT+TGG | + | 51431:33100-33119 | None:intergenic | 40.0% | |
| GGCTGTATTGCTTATCATGA+AGG | - | 51431:33134-33153 | MS.gene061926:CDS | 40.0% | |
| GTACTTTGTTCTTGATGAGG+AGG | - | 51431:33752-33771 | MS.gene061926:CDS | 40.0% | |
| TCACCGCATTCATCTTCTTA+TGG | - | 51431:33113-33132 | MS.gene061926:CDS | 40.0% | |
| ! | CGGGTCCTTTTATCAAAGAT+TGG | - | 51431:33406-33425 | MS.gene061926:CDS | 40.0% |
| ! | TGTGATTCCTCTGCAAGTTT+TGG | - | 51431:33353-33372 | MS.gene061926:CDS | 40.0% |
| !!! | TTGTTTTGATCGGTACTGGT+TGG | - | 51431:33280-33299 | MS.gene061926:CDS | 40.0% |
| AAGCAGCTCAAATTGCTCTC+AGG | - | 51431:33781-33800 | MS.gene061926:CDS | 45.0% | |
| AGCAGCTCAAATTGCTCTCA+GGG | - | 51431:33782-33801 | MS.gene061926:CDS | 45.0% | |
| GAATCTTATCTGTGCTGCAG+AGG | - | 51431:33161-33180 | MS.gene061926:CDS | 45.0% | |
| GGGAAATGTAGTGAGAATCG+AGG | + | 51431:32804-32823 | None:intergenic | 45.0% | |
| GTATTCGTTAGGGGTAGTGA+CGG | + | 51431:32887-32906 | None:intergenic | 45.0% | |
| TATTCGTTAGGGGTAGTGAC+GGG | + | 51431:32886-32905 | None:intergenic | 45.0% | |
| TCTAAAGACTATCTCTCCGC+TGG | - | 51431:32991-33010 | MS.gene061926:CDS | 45.0% | |
| TGTTCTTGATGAGGAGGAAG+AGG | - | 51431:33758-33777 | MS.gene061926:CDS | 45.0% | |
| TTCCGTTGTGATTGGTGAGA+CGG | - | 51431:33386-33405 | MS.gene061926:CDS | 45.0% | |
| !! | AAGGTCACTGGTTTACCTCA+TGG | - | 51431:33198-33217 | MS.gene061926:CDS | 45.0% |
| !! | CACTGGTTTACCTCATGGAT+GGG | - | 51431:33203-33222 | MS.gene061926:CDS | 45.0% |
| !! | TCACTGGTTTACCTCATGGA+TGG | - | 51431:33202-33221 | MS.gene061926:CDS | 45.0% |
| !!! | GGTTTTGTTGGATCTCGATG+AGG | + | 51431:32768-32787 | None:intergenic | 45.0% |
| AATCGAGGACGCAGAAGGAA+GGG | + | 51431:32789-32808 | None:intergenic | 50.0% | |
| ACCCGTCTCACCAATCACAA+CGG | + | 51431:33391-33410 | None:intergenic | 50.0% | |
| AGGGAGTTGAGTTTGTCCAG+CGG | + | 51431:33010-33029 | None:intergenic | 50.0% | |
| ATTCGTTAGGGGTAGTGACG+GGG | + | 51431:32885-32904 | None:intergenic | 50.0% | |
| CTCGATGAGGACTTGGATGA+GGG | + | 51431:32755-32774 | None:intergenic | 50.0% | |
| GCGAACGAACATGGAAACAG+AGG | + | 51431:32941-32960 | None:intergenic | 50.0% | |
| GGATGAGGGTTTCTTCGTTG+AGG | + | 51431:32741-32760 | None:intergenic | 50.0% | |
| GGTCACGGAAAGTGAAGAGA+CGG | + | 51431:32825-32844 | None:intergenic | 50.0% | |
| GTCACGGAAAGTGAAGAGAC+GGG | + | 51431:32824-32843 | None:intergenic | 50.0% | |
| GTTGGATCTCGATGAGGACT+TGG | + | 51431:32762-32781 | None:intergenic | 50.0% | |
| TCCGTTGTGATTGGTGAGAC+GGG | - | 51431:33387-33406 | MS.gene061926:CDS | 50.0% | |
| TCTCGATGAGGACTTGGATG+AGG | + | 51431:32756-32775 | None:intergenic | 50.0% | |
| TGAGGAGGAAGAAACCGAGA+CGG | + | 51431:32723-32742 | None:intergenic | 50.0% | |
| TGAGGGTTTCTTCGTTGAGG+AGG | + | 51431:32738-32757 | None:intergenic | 50.0% | |
| ! | AATCTCGGTGCGAACGAACA+TGG | + | 51431:32950-32969 | None:intergenic | 50.0% |
| !! | CGCAGAAGGAAGGGTTTTGT+TGG | + | 51431:32780-32799 | None:intergenic | 50.0% |
| AAACCGAGACGGGAGGATTC+GGG | + | 51431:32712-32731 | None:intergenic | 55.0% | |
| AGAGCGGTTGAAGGAAGCTG+AGG | + | 51431:32860-32879 | None:intergenic | 55.0% | |
| GAAACCGAGACGGGAGGATT+CGG | + | 51431:32713-32732 | None:intergenic | 55.0% | |
| GAATCGAGGACGCAGAAGGA+AGG | + | 51431:32790-32809 | None:intergenic | 55.0% | |
| GAGCGGTTGAAGGAAGCTGA+GGG | + | 51431:32859-32878 | None:intergenic | 55.0% | |
| GAGGAGGAAGAAACCGAGAC+GGG | + | 51431:32722-32741 | None:intergenic | 55.0% | |
| GTGAGAATCGAGGACGCAGA+AGG | + | 51431:32794-32813 | None:intergenic | 55.0% | |
| TTGAAGGAAGCTGAGGGAGG+AGG | + | 51431:32853-32872 | None:intergenic | 55.0% | |
| ! | AGTGACGGAGACAGAGGAGA+CGG | + | 51431:32668-32687 | None:intergenic | 55.0% |
| ! | GAGATGGTGACGTGGCCTTT+GGG | + | 51431:32646-32665 | None:intergenic | 55.0% |
| AAGCTGAGGGAGGAGGAGAG+AGG | + | 51431:32846-32865 | None:intergenic | 60.0% | |
| AGGGAGGAGGAGAGAGGTCA+CGG | + | 51431:32840-32859 | None:intergenic | 60.0% | |
| CAACCCGAATCCTCCCGTCT+CGG | - | 51431:32706-32725 | MS.gene061926:CDS | 60.0% | |
| CGGTTGAAGGAAGCTGAGGG+AGG | + | 51431:32856-32875 | None:intergenic | 60.0% | |
| GACGGGGTAAGAGCGGTTGA+AGG | + | 51431:32869-32888 | None:intergenic | 60.0% | |
| GAGGAAGAAACCGAGACGGG+AGG | + | 51431:32719-32738 | None:intergenic | 60.0% | |
| GGAGACAGAGGAGACGGAGA+TGG | + | 51431:32662-32681 | None:intergenic | 60.0% | |
| GGAGGAGGAGAGAGGAGTGA+CGG | + | 51431:32683-32702 | None:intergenic | 60.0% | |
| ! | AGGAGACGGAGATGGTGACG+TGG | + | 51431:32654-32673 | None:intergenic | 60.0% |
| ! | GAGACGGGAGGATTCGGGTT+GGG | + | 51431:32707-32726 | None:intergenic | 60.0% |
| ! | GAGAGGAGTGACGGAGACAG+AGG | + | 51431:32674-32693 | None:intergenic | 60.0% |
| ! | GGAGATGGTGACGTGGCCTT+TGG | + | 51431:32647-32666 | None:intergenic | 60.0% |
| ! | GGGTAGTGACGGGGTAAGAG+CGG | + | 51431:32876-32895 | None:intergenic | 60.0% |
| CGAGACGGGAGGATTCGGGT+TGG | + | 51431:32708-32727 | None:intergenic | 65.0% | |
| GGTTGGGAGGAGGAGGAGAG+AGG | + | 51431:32691-32710 | None:intergenic | 65.0% | |
| !! | ACGGGAGGATTCGGGTTGGG+AGG | + | 51431:32704-32723 | None:intergenic | 65.0% |
| !! | GGAGGATTCGGGTTGGGAGG+AGG | + | 51431:32701-32720 | None:intergenic | 65.0% |
| !! | GGATTCGGGTTGGGAGGAGG+AGG | + | 51431:32698-32717 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51431 | gene | 32613 | 33824 | 32613 | ID=MS.gene061926 |
| 51431 | mRNA | 32613 | 33824 | 32613 | ID=MS.gene061926.t1;Parent=MS.gene061926 |
| 51431 | exon | 32613 | 33824 | 32613 | ID=MS.gene061926.t1.exon1;Parent=MS.gene061926.t1 |
| 51431 | CDS | 32613 | 33824 | 32613 | ID=cds.MS.gene061926.t1;Parent=MS.gene061926.t1 |
| Gene Sequence |
| Protein sequence |
